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https://www.readbyqxmd.com/read/28107425/using-an-inducible-promoter-of-a-gene-encoding-penicillium-verruculosum-glucoamylase-for-production-of-enzyme-preparations-with-enhanced-cellulase-performance
#1
Alexander G Bulakhov, Pavel V Volkov, Aleksandra M Rozhkova, Alexander V Gusakov, Vitaly A Nemashkalov, Aidar D Satrutdinov, Arkady P Sinitsyn
BACKGROUND: Penicillium verruculosum is an efficient producer of highly active cellulase multienzyme system. One of the approaches for enhancing cellulase performance in hydrolysis of cellulosic substrates is to enrich the reaction system with β -glucosidase and/or accessory enzymes, such as lytic polysaccharide monooxygenases (LPMO) displaying a synergism with cellulases. RESULTS: Genes bglI, encoding β-glucosidase from Aspergillus niger (AnBGL), and eglIV, encoding LPMO (formerly endoglucanase IV) from Trichoderma reesei (TrLPMO), were cloned and expressed by P...
2017: PloS One
https://www.readbyqxmd.com/read/28107376/deletion-of-ph-regulator-pac-3-affects-cellulase-and-xylanase-activity-during-sugarcane-bagasse-degradation-by-neurospora-crassa
#2
Amanda Cristina Campos Antoniêto, Wellington Ramos Pedersoli, Lílian Dos Santos Castro, Rodrigo da Silva Santos, Aline Helena da Silva Cruz, Karoline Maria Vieira Nogueira, Rafael Silva-Rocha, Antonio Rossi, Roberto Nascimento Silva
Microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. The filamentous fungus Neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. The production of cellulases and hemicellulases is known to be affected by the environmental pH; however, the regulatory mechanisms of this process are still poorly understood. In this study, we investigated the role of the pH regulator PAC-3 in N. crassa during their growth on sugarcane bagasse at different pH conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28106512/endoglucanase-and-xylanase-production-by-bacillus-sp-ar03-in-co-culture-glycosyl-hydrolases-by-bacillus-sp-ar03-in-co-culture
#3
Johan S Hero, José H Pisa, Nora I Perotti, Cintia M Romero, María A Martínez
The behaviour of three isolates retrieved from different cellulolytic consortia, Bacillus sp. AR03, Paenibacillus sp. AR247 and Achromobacter sp. AR476-2, were examined individually and as co-cultures in order evaluate their ability to produce extracellular cellulases and xylanases. Utilizing a peptone-based medium supplemented with carboxymethyl cellulose (CMC), an increase estimated of 1.30 and 1.50 times was obtained by the co-culture containing the strains AR03 and AR247, with respect to enzyme titles registered by their individual cultivation...
January 20, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28104900/extremozymes-a-potential-source-for-industrial-applications
#4
REVIEW
Kelly Dumorné, David Camacho Córdova, Marcia Astorga-Eló, Prabhaharan Renganathan
Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, possess extraordinary properties are salt allowance thermostability and cold adaptivity. Extremozymes are very resistant in extreme conditions due to their great solidity and they propose new opportunities for biocatalysis and biotransformations, also for the development of the economy and new line of research through their application...
January 20, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28101807/direct-bioethanol-production-from-wheat-straw-using-xylose-glucose-co-fermentation-by-co-culture-of-two-recombinant-yeasts
#5
Yuanyuan Zhang, Caiyun Wang, Lulu Wang, Ruoxin Yang, Peilei Hou, Junhong Liu
To achieve a cost-effective bioconversion of lignocellulosic materials, a novel xylose/glucose co-fermentation process by co-culture of cellulose-utilizing recombinant Saccharomyces cerevisiae (S. cerevisiae) and xylan-utilizing recombinant Pichia pastoris (P. pastoris) was developed, in which ethanol was produced directly from wheat straw without additional hydrolytic enzymes. Recombinant S. cerevisiae coexpressing three types of cellulase and recombinant P. pastoris coexpressing two types of xylanase were constructed, respectively...
January 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28101462/identification-and-molecular-characterization-of-genes-coding-pharmaceutically-important-enzymes-from-halo-thermo-tolerant-bacillus
#6
Azam Safary, Rezvan Moniri, Maryam Hamzeh-Mivehroud, Siavoush Dastmalchi
Purpose: Robust pharmaceutical and industrial enzymes from extremophile microorganisms are main source of enzymes with tremendous stability under harsh conditions which make them potential tools for commercial and biotechnological applications. Methods: The genome of a Gram-positive halo-thermotolerant Bacillus sp. SL1, new isolate from Saline Lake, was investigated for the presence of genes coding for potentially pharmaceutical enzymes. We determined gene sequences for the enzymes laccase (CotA), l-asparaginase (ansA3, ansA1), glutamate-specific endopeptidase (blaSE), l-arabinose isomerase (araA2), endo-1,4-β mannosidase (gmuG), glutaminase (glsA), pectate lyase (pelA), cellulase (bglC1), aldehyde dehydrogenase (ycbD) and allantoinases (pucH) in the genome of Bacillus sp...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#7
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28096492/identification-of-glutaminyl-cyclase-genes-involved-in-pyroglutamate-modification-of-fungal-lignocellulolytic-enzymes
#8
Vincent W Wu, Craig M Dana, Anthony T Iavarone, Douglas S Clark, N Louise Glass
: The breakdown of plant biomass to simple sugars is essential for the production of second-generation biofuels and high-value bioproducts. Currently, enzymes produced from filamentous fungi are used for deconstructing plant cell wall polysaccharides into fermentable sugars for biorefinery applications. A post-translational N-terminal pyroglutamate modification observed in some of these enzymes occurs when N-terminal glutamine or glutamate is cyclized to form a five-membered ring. This modification has been shown to confer resistance to thermal denaturation for CBH-1 and EG-1 cellulases...
January 17, 2017: MBio
https://www.readbyqxmd.com/read/28092734/combined-utilization-of-nutrients-and-sugar-derived-from-wheat-bran-for-d-lactate-fermentation-by-sporolactobacillus-inulinus-ybs1-5
#9
Jiahuang Li, Junfei Sun, Bin Wu, Bingfang He
To decrease d-Lactate production cost, wheat bran, a low-cost waste of milling industry, was selected as the sole feedstock. First, the nutrients were recovered from wheat bran by acid protease hydrolysis. Then, cellulosic hydrolysates were prepared from protease-treated samples after acid pretreatment and enzymatic saccharification. The combined use of nutrients and hydrolysates as nitrogen and carbon sources for fermentation by S. inulinus YB1-5 resulted in d-Lactate levels of 99.5g/L, with an average production efficiency of 1...
December 30, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#10
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28087533/polysaccharide-degradation-capability-of-actinomycetales-soil-isolates-from-a-semi-arid-grassland-of-the-colorado-plateau
#11
Chris M Yeager, La Verne Gallegos-Graves, John Dunbar, Cedar N Hesse, Hajnalka Daligault, Cheryl R Kuske
: Among the bacteria, members of the Actinomycetales are considered quintessential degraders of complex polysaccharides in soils. However, studies examining complex polysaccharide degradation by Actinomycetales (other than Streptomyces spp.) in soils are limited. Here, we examine the lignocellulolytic and chitinolytic potential of 112 Actinomycetales strains, encompassing 13 families, isolated from a semi-arid grassland of the Colorado Plateau, Utah, USA. Members of the Streptomycetaceae, Pseudonocardiaceae, Micromonosporaceae and Promicromonosporaceae exhibited robust activity against carboxymethylcellulose, xylan, chitin and pectin substrates (except for low/no pectinase activity by the Micromonosporaceae)...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28081524/effects-of-acidified-aqueous-glycerol-and-glycerol-carbonate-pretreatment-of-rice-husk-on-the-enzymatic-digestibility-structural-characteristics-and-bioethanol-production
#12
Majid Ebrahimi, Oliver B Villaflores, Emma E Ordono, Alvin R Caparanga
Rice husk as an abundant biomass was used in this study, and it contained 30.1% glucan and 13.5% xylan, 22.4% lignin. The pretreated rice husk with glycerol carbonate and acidified aqueous glycerol (10% water) at 90°C and 130°C for 60min had the maximum yield of glucan digestibility which was 78.2% and 69.7% respectively, using cellulase for 72h. The simultaneous saccharification and fermentation was conducted anaerobically at 37°C with Saccharomyces cerevisiae, 5% w/v glucan and 10FPU/g glucan of cellulase...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28078651/fed-batch-enzymatic-saccharification-of-high-solids-pretreated-lignocellulose-for-obtaining-high-titers-and-high-yields-of-glucose
#13
Young Hoon Jung, Hyun Min Park, Dong Hyun Kim, Jungwoo Yang, Kyoung Heon Kim
To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132...
January 11, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28078400/characterization-of-four-endophytic-fungi-as-potential-consolidated-bioprocessing-hosts-for-conversion-of-lignocellulose-into-advanced-biofuels
#14
Weihua Wu, Ryan W Davis, Mary Bao Tran-Gyamfi, Alan Kuo, Kurt LaButti, Sirma Mihaltcheva, Hope Hundley, Mansi Chovatia, Erika Lindquist, Kerrie Barry, Igor V Grigoriev, Bernard Henrissat, John M Gladden
Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called "mycodiesel," while growing on lignocellulosic plant and agricultural residues. The fact that endophytes are plant symbionts suggests that some may be able to produce lignocellulolytic enzymes, making them capable of both deconstructing lignocellulose and converting it into mycodiesel, two properties that indicate that these strains may be useful consolidated bioprocessing (CBP) hosts for the biofuel production...
January 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28074108/cellulolytic-robolector-towards-an-automated-high-throughput-screening-platform-for-recombinant-cellulase-expression
#15
Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur, Jochen Büchs
BACKGROUND: Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28071716/lytic-polysaccharide-monooxygenases-disrupt-the-cellulose-fibers-structure
#16
Ana Villares, Céline Moreau, Chloé Bennati-Granier, Sona Garajova, Loïc Foucat, Xavier Falourd, Bodo Saake, Jean-Guy Berrin, Bernard Cathala
Lytic polysaccharide monooxygenases (LPMOs) are a class of powerful oxidative enzymes that breakdown recalcitrant polysaccharides such as cellulose. Here we investigate the action of LPMOs on cellulose fibers. After enzymatic treatment and dispersion, LPMO-treated fibers show intense fibrillation. Cellulose structure modifications visualized at different scales indicate that LPMO creates nicking points that trigger the disintegration of the cellulose fibrillar structure with rupture of chains and release of elementary nanofibrils...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071001/purified-isolation-of-vacuoles-from-sedum-alfredii-leaf-derived-protoplasts
#17
Xiao-Yu Gao, Xing-Cheng Liao, Ruo-Lai Wu, Ting Liu, Hai-Xing Wang, Ling-Li Lu
This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macerozyme at 50 °C for 5 min significantly accelerated the cell wall degradation. For the most optimal conditions for mesophyll protoplast isolation, the mixture of fresh leaves and cell lysates was followed by a 2-h-long vibration. The protoplast lysate for vacuole isolation was diluted, and 0.675 mmol/L was identified as the most appropriate 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonic acid (CHAPS) level, in which S...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28069197/low-strength-ultrasonication-positively-affects-methanogenic-granules-toward-higher-ad-performance-hydrolytic-enzyme-excretions
#18
Si-Kyung Cho, Yeo-Myeong Yun, Seung Gu Shin
In our previous studies, enhanced methane (CH4) production using low-strength ultrasonication was achieved and the results were evidenced by physico-chemical and molecular biological approaches. As a final continuation study, the effects of low-strength ultrasonication on the activities of hydrolytic enzymes (amylase, cellulase, and protease) were investigated on methanogenic granules given that hydrolysis regulates the whole anaerobic digestion (AD) process. Up to 213% enhanced hydrolytic enzyme activities were observed, and they seem to be highly related to the enhanced CH4 production...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28068666/mitigation-of-membrane-biofouling-in-mbr-using-a-cellulolytic-bacterium-undibacterium-sp-dm-1-isolated-from-activated-sludge
#19
Chang Hyun Nahm, Seonki Lee, Sang Hyun Lee, Kibaek Lee, Jaewoo Lee, Hyeokpil Kwon, Kwang-Ho Choo, Jung-Kee Lee, Jae Young Jang, Chung-Hak Lee, Pyung-Kyu Park
Biofilm formation on membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium, Undibacterium sp. DM-1, was isolated from a lab-scale MBR for wastewater treatment...
January 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28068591/feasibility-of-reusing-the-black-liquor-for-enzymatic-hydrolysis-and-ethanol-fermentation
#20
Wen Wang, Xiaoyan Chen, Xuesong Tan, Qiong Wang, Yunyun Liu, Minchao He, Qiang Yu, Wei Qi, Yu Luo, Xinshu Zhuang, Zhenhong Yuan
The black liquor (BL) generated in the alkaline pretreatment process is usually thought as the environmental pollutant. This study found that the pure alkaline lignin hardly inhibited the enzymatic hydrolysis of cellulose (EHC), which led to the investigation on the feasibility of reusing BL as the buffer via pH adjustment for the subsequent enzymatic hydrolysis and fermentation. The pH value of BL was adjusted from 13.23 to 4.80 with acetic acid, and the alkaline lignin was partially precipitated. It deposited on the surface of cellulose and negatively influenced the EHC via blocking the access of cellulase to cellulose and adsorbing cellulase...
December 23, 2016: Bioresource Technology
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