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https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#1
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28528086/cellulosic-ethanol-status-and-innovation
#2
REVIEW
Lee R Lynd, Xiaoyu Liang, Mary J Biddy, Andrew Allee, Hao Cai, Thomas Foust, Michael E Himmel, Mark S Laser, Michael Wang, Charles E Wyman
Although the purchase price of cellulosic feedstocks is competitive with petroleum on an energy basis, the cost of lignocellulose conversion to ethanol using today's technology is high. Cost reductions can be pursued via either in-paradigm or new-paradigm innovation. As an example of new-paradigm innovation, consolidated bioprocessing using thermophilic bacteria combined with milling during fermentation (cotreatment) is analyzed. Acknowledging the nascent state of this approach, our analysis indicates potential for radically improved cost competitiveness and feasibility at smaller scale compared to current technology, arising from (a) R&D-driven advances (consolidated bioprocessing with cotreatment in lieu of thermochemical pretreatment and added fungal cellulase), and (b) configurational changes (fuel pellet coproduction instead of electricity, gas boiler(s) in lieu of a solid fuel boiler)...
May 18, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28527996/enzymatic-hydrolysis-of-pretreated-alfa-fibers-stipa-tenacissima-using-%C3%AE-d-glucosidase-and-xylanase-of-talaromyces-thermophilus-from-solid-state-fermentation
#3
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Kamel Walha, Hajer Hassairi, Ali Gargouri, Hafedh Belghith
This work aims at realizing an optimal hydrolysis of pretreated Alfa fibers (Stipa tenacissima) through the use of enzymes produced from Talaromyces thermophilus AX4, namely β-D-glucosidase and xylanase, by a solid state fermentation process of an agro-industrial waste (wheat bran supplemented with lactose). The carbon source was firstly selected and the optimal values of three other parameters were determined: substrate loading (10g), moisture content (85%) and production time (10 days); which led to an optimized enzymatic juice...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#4
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28523288/extracellular-xylanopectinolytic-enzymes-by-bacillus-subtilis-adi1-from-efb-s-compost
#5
Muhammad Hariadi Nawawi, Rosfarizan Mohamad, Paridah Md Tahir, Wan Zuhainis Saad
Microbial xylanase and pectinase are two extremely valuable enzymes, which have captivated much attention. This can be seen from the increased demand for these enzymes by many industrial sectors. This study investigates the isolation and screening of extracellular xylanopectinolytic enzymes-producing bacteria in a submerged fermentation (SmF). Samples are collected from the compost of empty fruit bunch (EFB) at Biocompost Pilot Plant, located at Biorefinery Plant, Universiti Putra Malaysia. From the experiment, out of 20 isolates, 11 isolates show xylanase or/and pectinase activity, and only one isolate (EFB-11) shows the concurrent activities of xylanase and pectinase...
2017: International Scholarly Research Notices
https://www.readbyqxmd.com/read/28523076/metaproteomics-guided-selection-of-targeted-enzymes-for-bioprospecting-of-mixed-microbial-communities
#6
Jutta Speda, Bengt-Harald Jonsson, Uno Carlsson, Martin Karlsson
BACKGROUND: Hitherto, the main goal of metaproteomic analyses has been to characterize the functional role of particular microorganisms in the microbial ecology of various microbial communities. Recently, it has been suggested that metaproteomics could be used for bioprospecting microbial communities to query for the most active enzymes to improve the selection process of industrially relevant enzymes. In the present study, to reduce the complexity of metaproteomic samples for targeted bioprospecting of novel enzymes, a microbial community capable of producing cellulases was maintained on a chemically defined medium in an enzyme suppressed metabolic steady state...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28522200/biological-disintegration-of-microalgae-for-biomethane-recovery-prediction-of-biodegradability-and-computation-of-energy-balance
#7
S Kavitha, R Yukesh Kannah, J Rajesh Banu, S Kaliappan, M Johnson
The present study investigates the synergistic effect of combined bacterial disintegration on mixed microalgal biomass for energy efficient biomethane generation. The rate of microalgal biomass lysis, enhanced biodegradability, and methane generation were used as indices to assess efficiency of the disintegration. A maximal dissolvable organics release and algal biomass lysis rate of about 1100, 950 and 800mg/L and 26, 23 and 18% was achieved in PA+C (protease, amylase+cellulase secreting bacteria), C (cellulase alone) and PA (protease, amylase) microalgal disintegration...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28512464/insights-into-the-mechanism-of-proliferation-on-the-special-microbes-mediated-by-phenolic-acids-in-the-radix-pseudostellariae-rhizosphere-under-continuous-monoculture-regimes
#8
Hongmiao Wu, Junjian Xu, Juanying Wang, Xianjin Qin, Linkun Wu, Zhicheng Li, Sheng Lin, Weiwei Lin, Quan Zhu, Muhammad U Khan, Wenxiong Lin
As potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium Kosakonia sacchari and beneficial bacterium Bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of R. pseudostellariae in the monoculture system...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28511694/ethephon-induced-oxidative-stress-in-the-olive-leaf-abscission-zone-enables-development-of-a-selective-abscission-compound
#9
S Goldental-Cohen, C Burstein, I Biton, S Ben Sasson, A Sadeh, Y Many, A Doron-Faigenboim, H Zemach, Y Mugira, D Schneider, R Birger, S Meir, S Philosoph-Hadas, V Irihomovitch, S Lavee, B Avidan, G Ben-Ari
BACKGROUND: Table olives (Olea europaea L.), despite their widespread production, are still harvested manually. The low efficiency of manual harvesting and the rising costs of labor have reduced the profitability of this crop. A selective abscission treatment, inducing abscission of fruits but not leaves, is crucial for the adoption of mechanical harvesting of table olives. In the present work we studied the anatomical and molecular differences between the three abscission zones (AZs) of olive fruits and leaves...
May 16, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28504183/enzymic-degradation-of-hydroxyethyl-cellulose-and-analysis-of-the-substitution-pattern-along-the-polysaccharide-chain
#10
Yongfu Diao, Mingwei Song, Yulin Zhang, Lin-Ying Shi, Yusan Lv, Rong Ran
The enzymatic degradation behavior of hydroxyethyl cellulose (HEC) samples with different molar substitutions (MS) values was investigated. The changes in the molecular structure of HEC treated with enzymatic approach in comparison to the native HEC were studied through nuclear magnetic resonance (NMR), fourier transform infrared spectra (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques and kinetics of degradation was studied by viscometry. The cleavage of HEC chains could be observed from FTIR and kinetics results...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28503798/neutral-and-alkaline-cellulases-production-engineering-and-applications
#11
REVIEW
Ines Ben Hmad, Ali Gargouri
Neutral and alkaline cellulases from microorganisms constitute a major group of the industrial enzymes and find applications in various industries. Screening is the important ways to get novel cellulases. Most fungal cellulases have acidic pH optima, except some fungi like Humicola insolens species. However, new applications require the use of neutral and alkaline cellulases in food, brewery and wine, animal feed, textile and laundry, pulp and paper industries, agriculture as well in scientific research purposes...
May 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28503710/characterization-of-a-new-multifunctional-beta-glucosidase-from-musca-domestica
#12
Shu Zhang, Jian Huang, Rong Hu, Guo Guo, Xiaoli Shang, Jianwei Wu
OBJECTIVE: To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS: A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked to pPICZαA and expressed in P. pastoris with a yield of 500 mg l(-1). The enzyme has the maximum activity with 27.6 U mg(-1) towards cellulose...
May 13, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28502854/characterization-of-a-bi-functional-cellulase-produced-by-a-gut-bacterial-resident-of-rosaceae-branch-borer-beetle-osphranteria-coerulescens-coleoptera-cerambycidae
#13
Atousa Hatefi, Ali Makhdoumi, Ahmad Asoodeh, Omid Mirshamsi
A cellulolytic bacterium was obtained from the digestive tract of Osphranteria coerulescens. The breakdown of woody and cellulosic substances by this insect may be relative in part to its symbiont bacteria. Under optimal cultural conditions the novel isolate produced 5.35U/ml cellulase after 72h. The enzyme was purified to 36 fold with a 0.59% yield and showed a specific activity of 9.0U/mg. It presented its maximum activity at 60°C and pH 5, while it was stable in a wide range of temperature from 20 to 60°C and pH from 5 to 10...
May 11, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28501684/ultrasonic-hyperactivation-of-cellulase-immobilized-on-magnetic-nanoparticles
#14
Mayur Ramrao Ladole, Jayesh Sevantilal Mevada, Aniruddha Bhalchandra Pandit
In the present work, effect of low power, low frequency ultrasound on cellulase immobilized magnetic nanoparticles (cellulase@MNPs) was studied. To gain maximum activity recovery in cellulase@MNPs various parameters viz. ratio of MNPs:cellulase, concentration of glutaraldehyde and cross-linking time were optimized. The influence of ultrasonic power on cellulase@MNPs was studied. Under ultrasonic conditions at 24kHz, 6W power, and 6min of incubation time there was almost 3.6 fold increased in the catalytic activity of immobilized cellulase over the control...
April 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28497120/analysis-of-light-and-carbon-specific-transcriptomes-implicates-a-class-of-g-protein-coupled-receptors-in-cellulose-sensing
#15
Eva Stappler, Christoph Dattenböck, Doris Tisch, Monika Schmoll
In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation levels of the transcriptome of T. reesei: light regulation and carbon source-dependent control. We show that the carbon source (cellulose, lactose, sophorose, glucose, or glycerol) is the major source of variation, with light having a modulating effect on transcript regulation...
May 2017: MSphere
https://www.readbyqxmd.com/read/28495621/a-reusable-multipurpose-magnetic-nanobiocatalyst-for-industrial-applications
#16
Mohammad Perwez, Razi Ahmad, Meryam Sardar
A multipurpose magnetic nanobiocatalyst is developed by conjugating Pectinex 3XL (a commercial enzyme containing pectinase, xylanase and cellulase activities) on 3-aminopropyl triethoxysilane activated magnetic nanoparticles. The nanobiocatalyst retained 87% of pectinase, 69% of xylanase and 58% of cellulase activity after conjugation on modified nanoparticles as compared to their soluble counterparts. Thermal stability data at 70°C showed increase in enzyme stability after conjugation to nanoparticles and the kinetic parameters (Km and Vmax) remain unaltered after immobilization...
May 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28489493/characterization-of-cellulolytic-enzyme-system-of-schizophyllum-commune-mutant-and-evaluation-of-its-efficiency-on-biomass-hydrolysis
#17
Warasirin Sornlake, Phatcharamon Rattanaphanjak, Verawat Champreda, Lily Eurwilaichitr, Suthathip Kittisenachai, Sittiruk Roytrakul, Tatsuya Fujii, Hiroyuki Inoue
Schizophyllum commune is a basidiomycete equipped with an efficient cellulolytic enzyme system capable of growth on decaying woods. In this study, production of lignocellulose-degrading enzymes from S. commune mutant G-135 (SC-Cel) on various cellulosic substrates was examined. The highest cellulase activities including CMCase, FPase, and β-glucosidase were obtained on Avicel-PH101 while a wider range of enzymes attacking non-cellulosic polysaccharides and lignin were found when grown on alkaline-pretreated biomass...
May 10, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28488758/engineered-humicola-insolens-cutinase-for-efficient-cellulose-acetate-deacetylation
#18
Abhijit N Shirke, Glenn L Butterfoss, Rakhi Saikia, Aditya Basu, Leonardo de Maria, Allan Svendsen, Richard A Gross
Cutinases comprise a family of esterases with broad hydrolytic activity for chain and pendant ester groups. This work aimed to identify and improve an efficient cutinase for cellulose acetate (CA) deacetylation. The development of a mild method for CA fiber surface deacetylation will result in improved surface hydrophilicity and reactivity while, when combined with cellulases, a route to the full recycling of CA to acetate and glucose. In this study, the comparative CA deacetylation activity of 4 homologous wild-type (wt) fungal cutinases from Aspergillus oryzae (AoC), Thiellavia terrestris (TtC), Fusarium solani (FsC), and Humicola insolens (HiC) was determined by analysis of CA deacetylation kinetics...
May 10, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28486197/on-site-cellulase-production-and-efficient-saccharification-of-corn-stover-employing-cbh2-overexpressing-trichoderma-reesei-with-novel-induction-system
#19
Yonghao Li, Xiaoyue Zhang, Liang Xiong, Muhammad Aamer Mehmood, Xinqing Zhao, Fengwu Bai
Although on-site cellulase production offers cost-effective saccharification of lignocellulosic biomass, low enzyme titer is still a barrier for achieving robustness. In the present study, a strain of T. reesei was developed for enhanced production of cellulase via overexpression of Cellobiohydrolase II. Furthermore, optimum enzyme production was achieved using a novel inducer mixture containing synthesized glucose-sophorose (MGD) and alkali pre-treated corn stover (APCS). Within 60h, a remarkably higher cellulase productivity and activity were achieved in the fed-batch fermentation using the optimized ratio of MGD and APCS in the inducer mixture, compared to those reported using cellulosic biomass as the sole inducer...
April 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28483534/comparative-secretomic-analysis-of-lignocellulose-degradation-by-lentinula-edodes-grown-on-microcrystalline-cellulose-lignosulfonate-and-glucose
#20
Yingli Cai, Yuhua Gong, Wei Liu, Yue Hu, Lianfu Chen, Lianlian Yan, Yan Zhou, Yinbing Bian
Lentinula edodes has the potential to degrade woody and nonwoody lignocellulosic biomass. However, the mechanism of lignocellulose degradation by L. edodes is unclear. The aim of this work is to explore the profiling of soluble secreted proteins involved in lignocellulose degradation in L. edodes. For that, we compared the secretomes of L. edodes grown on microcrystalline cellulose, cellulose with lignosulfonate and glucose. Based on nanoliquid chromatography coupled with tandem mass spectrometry of whole-protein hydrolysate, 230 proteins were identified...
May 5, 2017: Journal of Proteomics
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