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https://www.readbyqxmd.com/read/29791877/enzyme-assisted-extraction-chemical-characteristics-and-immunostimulatory-activity-of-polysaccharides-from-korean-ginseng-panax-ginseng-meyer
#1
Young-Ran Song, Su-Kyung Sung, Mi Jang, Tae-Gyu Lim, Chang-Won Cho, Chun-Ji Han, Hee-Do Hong
In this study, enzyme-assisted extraction was used to isolate functional polysaccharides from Korean ginseng (Panax ginseng Meyer) and the physicochemical and biological properties of the extracted polysaccharides were investigated, comparing with those from traditional hot-water extraction (FGWP). In macrophages, their effects on cytokines production could be ordered as FGEP-CA ≥ FGEP-A > FGEP-C > FGWP, suggesting that FGEP-CA (combined cellulase- and α-amylase-extracted polysaccharide) is a potent immunostimulator...
May 20, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29785688/the-influence-of-nonionic-surfactant-adsorption-on-enzymatic-hydrolysis-of-oil-palm-fruit-bunch
#2
Jatuporn Parnthong, Suratsawadee Kungsanant, Sumaeth Chavadej
Nonionic surfactants have been utilized to improve the enzymatic hydrolysis of lignocellulosic materials. However, the role of surfactant adsorption affecting enzymatic hydrolysis has not been elaborated well. In this work, nonionic surfactants differing in their molecular structures, namely the polyoxyethylene sorbitan monooleate (Tween 80), the secondary alcohol ethoxylate (Tergitol 15-S-9), and the branched alcohol ethoxylate (Tergitol TMN-6), were studied for their effects on the enzymatic hydrolysis of palm fruit bunch (PFB)...
May 22, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29785208/developing-cellulolytic-yarrowia-lipolytica-as-a-platform-for-the-production-of-valuable-products-in-consolidated-bioprocessing-of-cellulose
#3
Zhong-Peng Guo, Julien Robin, Sophie Duquesne, Michael Joseph O'Donohue, Alain Marty, Florence Bordes
Background: Both industrial biotechnology and the use of cellulosic biomass as feedstock for the manufacture of various commercial goods are prominent features of the bioeconomy. In previous work, with the aim of developing a consolidated bioprocess for cellulose bioconversion, we conferred cellulolytic activity of Yarrowia lipolytica , one of the most widely studied "nonconventional" oleaginous yeast species. However, further engineering this strain often leads to the loss of previously introduced heterologous genes due to the presence of multiple LoxP sites when using Cre -recombinase to remove previously employed selection markers...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29779122/heterologous-expression-of-talaromyces-emersonii-cellobiohydrolase-cel7a-in-trichoderma-reesei-increases-the-efficiency-of-corncob-residues-saccharification
#4
Ningning Sun, Yuanchao Qian, Weiwei Wang, Yaohua Zhong, Meixue Dai
OBJECTIVE: Improve the hydrolysis efficiency of the Trichoderma reesei cellulase system by heterologously expressing cellobiohydrolase Cel7A (Te-Cel7A) from the thermophilic fungus Talaromyces emersonii. RESULTS: Te-Cel7A was expressed in T. reesei under control of the cdna1 promoter and the generated transformant QTC14 could successfully secrete Te-Cel7A into the supernatant using glucose as carbon source. The recombinant Te-Cel7A had a temperature optimum at 65 °C and an optimal pH of 5, which were similar to those from the native host...
May 19, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29775905/kinetic-modelling-of-methane-production-during-bio-electrolysis-from-anaerobic-co-digestion-of-sewage-sludge-and-food-waste
#5
Kalp Bhusan Prajapati, Rajesh Singh
In present study batch tests were performed to investigate the enhancement in methane production under bio-electrolysis anaerobic co-digestion of sewage sludge and food waste. The bio-electrolysis reactor system (B-EL) yield more methane 148.5 ml/g COD in comparison to reactor system without bio-electrolysis (B-CONT) 125.1 ml/g COD. Whereas bio-electrolysis reactor system (C-EL) Iron Scraps amended yield lesser methane (51.2 ml/g COD) in comparison to control bio-electrolysis reactor system without Iron scraps (C-CONT - 114...
May 10, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29774620/cellulose-degradation-in-gastrophysa-viridula-coleoptera-chrysomelidae-functional-characterization-of-two-cazymes-belonging-to-glycoside-hydrolase-family-45-reveals-a-novel-enzymatic-activity
#6
André Busch, Grit Kunert, Natalie Wielsch, Yannick Pauchet
Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various metazoans, including leaf beetles, have been found to encode their own cellulases, giving them the ability to degrade cellulose independently of cellulolytic symbionts. Here, we analyzed the cellulosic capacity of the leaf beetle Gastrophysa viridula, which typically feeds on Rumex plants...
May 17, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29774473/genomic-and-probiotic-characterization-of-sjp-snu-strain-of-pichia-kudriavzevii
#7
Seung-Min Hong, Hyuk-Joon Kwon, Se-Joon Park, Won-Jin Seong, Ilhwan Kim, Jae-Hong Kim
The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces...
May 17, 2018: AMB Express
https://www.readbyqxmd.com/read/29774050/one-step-process-of-hydrothermal-and-alkaline-treatment-of-wheat-straw-for-improving-the-enzymatic-saccharification
#8
Shaolong Sun, Lidan Zhang, Fang Liu, Xiaolin Fan, Run-Cang Sun
Background: To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline treatment, which led to decrease the pure economic profit for production of bioethanol. Therefore, four one-step processes based on order of hydrothermal and alkaline treatment have been developed to enhance concentration of glucose of wheat straw by enzymatic saccharification...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29773074/enhanced-cellulase-production-in-trichoderma-reesei-rut-c30-via-constitution-of-minimal-transcriptional-activators
#9
Jiajia Zhang, Guoxiu Zhang, Wei Wang, Wei Wang, Dongzhi Wei
BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2 -VP16 and DBDcre1 -VP16) were designed and expressed in T...
May 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29767056/performance-and-ruminal-parameters-of-fattening-moghani-lambs-fed-recycled-poultry-bedding
#10
Ayoub Azizi, Afrooz Sharifi, Arash Azarfar, Ali Kiani, Alireza Jolazadeh
This study investigated the effects of recycled poultry bedding (RPB) on performance and protozoa population, microbial enzyme activity and microbial protein synthesis (MPS) in rumen contents of fattening lambs. Thirty-six male Moghani lambs (31.4 ± 3.2 kg body weight) were fed iso-energetic and iso-nitrogenous diets containing 0, 70, 140 or 210 g/kg dry matter (DM) RPB in a balanced randomized design (9 lambs per treatment). Results showed that final body weight, DM intake, average daily gain and feed conversion ratio were unchanged ( P  > 0...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766241/cellulase-production-by-white-rot-basidiomycetous-fungi-solid-state-versus-submerged-cultivation
#11
REVIEW
Joseph A Bentil, Anders Thygesen, Moses Mensah, Lene Lange, Anne S Meyer
White-rot basidiomycetous (WRB) fungi are a group of wood-decaying fungi that are known to be endowed with the ability to secrete enzymes that can catalyze decomposition of a range of plant cell wall polysaccharides, including cellulose and lignin. Expression of these enzymes is induced by the substrate and the enzyme yields obtained depend on the growth of the fungi and thus the mode of cultivation. In order to exploit WRB fungi for local enzyme production for converting lignocellulosic materials in biorefinery processes, the fungi can principally be cultivated in either solid-state (SSC) or submerged cultivation (SmC) systems...
May 15, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29765817/solid-state-fermentation-of-oil-palm-frond-petiole-for-lignin-peroxidase-and-xylanase-rich-cocktail-production
#12
Mohamed Roslan Mohamad Ikubar, Musaalbakri Abdul Manan, Madihah Md Salleh, Adibah Yahya
In current practice, oil palm frond leaflets and stems are re-used for soil nutrient recycling, while the petioles are typically burned. Frond petioles have high commercialization value, attributed to high lignocellulose fiber content and abundant of juice containing free reducing sugars. Pressed petiole fiber is the subject of interest in this study for the production of lignocellulolytic enzyme. The initial characterization showed the combination of 0.125 mm frond particle size and 60% moisture content provided a surface area of 42...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29764763/direct-recovery-of-mangostins-from-garcinia-mangostana-pericarps-using-cellulase-assisted-aqueous-micellar-biphasic-system-with-recyclable-surfactant
#13
Hui-Suan Ng, Grace Yin Tze Tan, Ka-Heng Lee, Wolfgang Zimmermann, Hip Seng Yim, John Chi-Wei Lan
The α- and γ-mangostins from Garcinia mangostana pericarps (GMP) exhibit antioxidant, anti-bacterial, anti-inflammatory and anti-tumor properties. The extraction yields α- and γ-mangostins are often limited by the presence of the GMP cell walls. Therefore, the extraction and recovery of mangostins from GMP with an Aspergillus niger cellulase-assisted aqueous micellar biphasic system (CA-AMBS) was developed for enhanced yield of mangostins. Effects of the concentration of cellulase, the incubation time and the temperature of the system on the recovery of mangostins were investigated...
May 12, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29763800/potentials-of-using-mixed-culture-bacteria-incorporated-with-sodium-bicarbonate-for-hydrogen-production-from-water-hyacinth
#14
Alaa Wazeri, Mohamed Elsamadony, Sophie Le Roux, Pascal Peu, Ahmed Tawfik
The aim of this study is to assess the potentials of using mixed culture bacteria incorporated with different concentrations of NaHCO3 for hydrogen production from water hyacinth (WH). The lowest hydrogen yield (HY) of 30.4 ± 1.9 mL/gTVS , H2 content (HC) of 19.5 ± 1.5% and hydrogenase enzyme (HE) activity of 0.06 ± 0.01 mgM.Breduced /min were registered for the cultures without supplementation of NaHCO3 . The HY, HC, and HE activity were maximized at levels of 69.2 ± 4.3 mL/gTVS, 58...
May 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29760775/a-downstream-box-fusion-allows-stable-accumulation-of-a-bacterial-cellulase-in-chlamydomonas-reinhardtii-chloroplasts
#15
Lubna V Richter, Huijun Yang, Mohammad Yazdani, Maureen R Hanson, Beth A Ahner
Background: We investigated strategies to improve foreign protein accumulation in the chloroplasts of the model algae Chlamydomonas reinhardtii and tested the outcome in both standard culture conditions as well as one pertinent to algal biofuel production. The downstream box (DB) of the TetC or NPTII genes, the first 15 codons following the start codon, was N -terminally fused to the coding region of cel6A , an endoglucanase from Thermobifida fusca . We also employed a chimeric regulatory element, consisting of the 16S rRNA promoter and the atpA 5'UTR, previously reported to enhance protein expression, to regulate the expression of the TetC- cel6A gene...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29759794/effectiveness-of-cross-linked-enzyme-aggregates-of-cellulolytic-enzymes-in-hydrolyzing-wheat-straw
#16
Shuddhodana, Munishwar N Gupta, Virendra S Bisaria
Development of industrially potent cellulolytic enzymes is one of the greatest challenges faced in lignocellulosic feed-stock based bio-refining. In the current work cross-linked enzyme aggregates (CLEAs) of commercial cellulase mix were successfully prepared and their performance to be used as potential industrial enzymes in terms of stability and wheat straw hydrolysis was evaluated. The CLEAs were more stable compared to native enzymes with half-lives being 2.30-, 1.56-, 3.07- and 1.67-fold higher at 70°C for filter paper activity (FPA), endoglucanase, β-glucosidase and xylanase, respectively...
May 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29755582/rsm-based-optimization-of-nutritional-conditions-for-cellulase-mediated-saccharification-by-bacillus-cereus
#17
Fouzia Tabssum, Muhammad Irfan, Hafiz Abdullah Shakir, Javed Iqbal Qazi
Background: Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of Bacillus cereus was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimized through response surface methodology...
2018: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29754180/diversity-roles-and-biotechnological-applications-of-symbiotic-microorganisms-in-the-gut-of-termite
#18
REVIEW
Jing Zhou, Jiwei Duan, Mingkun Gao, Ying Wang, Xiaohua Wang, Kai Zhao
Termites are global pests and can cause serious damage to buildings, crops, and plantation forests. The symbiotic intestinal flora plays an important role in the digestion of cellulose and nitrogen in the life of termites. Termites and their symbiotic microbes in the gut form a synergistic system. These organism work together to digest lignocellulose to make the termites grow on nitrogen deficient food. In this paper, the diversity of symbiotic microorganisms in the gut of termites, including protozoan, spirochetes, actinomycetes, fungus and bacteria, and their role in the digestion of lignocellulose and also the biotechnological applications of these symbiotic microorganisms are discussed...
May 12, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29753933/co-immobilization-of-cellulase-and-lysozyme-on-amino-functionalized-magnetic-nanoparticles-an-activity-tunable-biocatalyst-for-extraction-of-lipids-from-microalgae
#19
Qingtai Chen, Dong Liu, Chongchong Wu, Kaisheng Yao, Zhiheng Li, Nan Shi, Fushan Wen, Ian D Gates
An activity-tunable biocatalyst for Nannochloropsis sp. cell-walls degradation was prepared by co-immobilization of cellulase and lysozyme on the surface of amino-functionalized magnetic nanoparticles (MNPs) employing glutaraldehyde. The competition between cellulase and lysozyme during immobilization was caused by the limited active sites of the MNPs. The maximum recovery of activities (cellulase: 78.9% and lysozyme: 69.6%) were achieved due to synergistic effects during dual-enzyme co-immobilization. The thermal stability in terms of half-life of the co-immobilized enzymes was three times higher than that in free form and had higher catalytic efficiency for hydrolysis of cell walls...
May 3, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29751295/the-availability-of-second-generation-feedstocks-for-the-treatment-of-acid-mine-drainage-and-to-improve-south-africa-s-bio-based-economy
#20
Dirk Karl Westensee, Karl Rumbold, Kevin G Harding, Craig M Sheridan, Lizelle D van Dyk, Geoffrey S Simate, Ferdinand Postma
South Africa has a wide range of mining activities making mineral resources important economic commodities. However, the industry is responsible for several environmental impacts; one of which is acid mine drainage (AMD). Despite several years of research, attempts to prevent AMD generation have proven to be difficult. Therefore, treatment of the resulting drainage has been common practice over the years. One of the recommended treatment methods is the use of second generation feedstocks (lignocellulosic biomass)...
May 8, 2018: Science of the Total Environment
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