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https://www.readbyqxmd.com/read/28318182/pneumococcal-meningitis-in-cantabria-spain-in-the-pneumococcal-conjugate-vaccine-era-2001-2015
#1
Esther González-Escartín, Itziar Angulo López, Elsa Ots Ruiz, Luis Martínez-Martínez, María J Cabero Pérez
OBJECTIVE: To analyze the characteristics of pneumococcal meningitis in children ≤ 14 years old following the market introduction of pneumococcal conjugate vaccines in our community. METHODS: Retrospective study of pneumococcal meningitis cases with a two-period analysis: pre-13-valent pneumococcal conjugate vaccine (PCV13) (2001-2010) and post-PCV13 (2010-2015). Patient demographic and clinical data, and microbiological data were collected. RESULTS: Eighteen cases were diagnosed...
April 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28315724/molecular-detection-of-six-virulence-genes-in-pseudomonas-aeruginosa-isolates-detected-in-children-with-urinary-tract-infection
#2
Ali Badamchi, Hossein Masoumi, Shima Javadinia, Ramin Asgarian, Azardokht Tabatabaee
Although a vast majority of Urinary tract infections (UTIs) are caused by E. coli, epidemiological reports have indicated an increasing rate of such infections caused by some other opportunistic organisms including Pseudomonas aeruginosa. Antimicrobial susceptibility and pathogenesis mechanisms of P. aeruginosa are poorly understood. The aim of this study was to detect some virulence factor genes and antimicrobial susceptibility patterns of P. aeruginosa isolates detected in patients with UTI, in children hospital of Tehran, Tehran, Iran...
March 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28288309/novel-fluorene-based-supramolecular-sensor-for-selective-detection-of-amoxicillin-in-water-and-blood
#3
Kiramat Shah, Erum Hassan, Farid Ahmed, Itrat Anis, Muhammad Rabnawaz, Muhammad Raza Shah
Synthesis, characterization and molecular recognition properties of fluorene based supramolecular cleft 1 is reported. The cleft molecule 1 was prepared in a single-step with good yield (85% yield), by linking Fluorene with 1-ethyl piperazine. The cleft molecule 1 was carefully characterized using various spectroscopic techniques such as NMR and mass spectrometry. The supramolecular interaction of cleft 1 with amoxicillin, 6APA, aspirin, captopril, cefotaxime, ceftriaxone, cefuroxime, diclofenac, penicillin, and cephradine was evaluated by fluorescent spectroscopy...
March 10, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28287269/a-critical-review-of-analytical-methods-for-quantification-of-cefotaxime
#4
Lívia Paganini Consortti, Hérida Regina Nunes Salgado
Bacterial resistance to antibiotics is a growing phenomenon in the world. Considering the relevance of antimicrobials for population and the reduction in the registration of new antimicrobials by regulatory, proper quality control is required in order to minimize the spread of bacterial resistance and ensure the effectiveness of a treatment, as well as safety for the patient. Among the antimicrobials is cefotaxime, a drug belonging to third-generation cephalosporins, which is highly active against Gram-negative bactéria and is used to treat central nervous system infections such as meningitis and septicemia...
March 13, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/28276133/plasmid-mediated-resistance-to-cephalosporins-and-quinolones-in-escherichia-coli-from-american-crows-in-the-usa
#5
Ivana Jamborova, Monika Dolejska, Ludek Zurek, Andrea K Townsend, Anne B Clark, Julie C Ellis, Ivo Papousek, Alois Cizek, Ivan Literak
American crow (Corvus brachyrhynchos) feces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 fecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays...
March 9, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28271652/impact-of-revised-broad-spectrum-cephalosporin-clinical-and-laboratory-standards-institute-breakpoints-on-susceptibility-in-enterobacteriaceae-producing-ampc-%C3%AE-lactamase
#6
Ki Ho Park, Yong Pil Chong, Sung Han Kim, Sang Oh Lee, Mi Suk Lee, Heungsup Sung, Mi Na Kim, Yang Soo Kim, Jun Hee Woo, Sang Ho Choi
We evaluated the impact of revised Clinical and Laboratory Standards Institute (CLSI) breakpoints for broad-spectrum cephalosporins (BSCs) on the susceptibilities of 1,742 isolates of Enterobacter species, Serratia marcescens, Citrobacter freundii, and Morganella morganii. The 2011 CLSI criteria for cefotaxime and ceftazidime reduced the rates of susceptibility by 2.9% and 5.9%, respectively. The 2014 CLSI criteria for cefepime reduced the rate of susceptibility by 13.9%, and categorized 11.8% isolates as susceptible-dose dependent (SDD) for cefepime...
February 28, 2017: Infection & Chemotherapy
https://www.readbyqxmd.com/read/28266280/additive-effect-of-mcp-in-combination-with-cefotaxime-against-staphylococcus-aureus
#7
Roula M Abdel-Massih, Elias Baydoun, Salah El-Khatib, Elias Dahdouh
BACKGROUND: Pectin is a heterogeneous polysaccharide present in plants and citrus fruits. It exhibits different beneficial biological activities. Conflicting reports exist about the antimicrobial effect of pectin and its derivatives. OBJECTIVE: In this study, we investigate the antimicrobial effect of Modified Citrus Pectin (MCP) against Staphylococcus aureus, a pathogen showing increasing rates of antimicrobial resistance worldwide. METHODS: Forty-three clinical isolates of S...
March 6, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28263245/the-treatment-of-possible-severe-infection-in-infants-an-open-randomized-safety-trial-of-parenteral-benzylpenicillin-and-gentamicin-versus-ceftriaxone-in-infants-60-days-of-age-in-malawi
#8
Elizabeth M Molyneux, Queen Dube, Francis Banda, Msandeni Chiume, Isaac Singini, Macpherson Mallewa, Edward C Schwalbe, Robert S Heyderman
BACKGROUND: The World Health Organization recommends benzylpenicillin and gentamicin as antimicrobial treatment of infants with sepsis in low income settings (LICs), and ceftriaxone or cefotaxime as an alternative. In a meta-analysis from 13 LICs, Staphylococcus aureus, Klebsiella spp. and E.coli accounted for 55% of infants with sepsis. In a review of bacterial meningitis, resistance to third generation cephalosporins was >50% of all isolates, and 44% of Gram-negative isolates were gentamicin resistant...
February 28, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28256474/long-term-outbreak-of-klebsiella-pneumoniae-third-generation-cephalosporin-use-in-a-neonatal-intensive-care-unit-in-north-india
#9
Tuhina Banerjee, Amitabha Bhattacharjee, Supriya Upadhyay, Shweta Mishra, Karuna Tiwari, Shampa Anupurba, Malay Ranjan Sen, Sriparna Basu, Ashok Kumar
BACKGROUND & OBJECTIVES: The indiscriminate use of third generation cephalosporin has contributed to the emergence and widespread dissemination of extended spectrum β lactamases (ESBL) genes in Klebsiella pneumoniae. This study was undertaken to elaborate the genetic behaviour of ESBL - producing K. pneumoniae isolates in the neonatal intensive care unit (NICU) of a tertiary care hospital in north India causing successive outbreaks in context with empirical third generation cephalosporin use...
October 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28243225/prevalence-and-molecular-characteristics-of-extended-spectrum-%C3%AE-lactamase-genes-in-escherichia-coli-isolated-from-diarrheic-patients-in-china
#10
Li Bai, Lili Wang, Xiaorong Yang, Juan Wang, Xin Gan, Wei Wang, Jin Xu, Qian Chen, Ruiting Lan, Séamus Fanning, Fengqin Li
Background: The emergence and spread of antimicrobial resistance has become a major global public health concern. A component element of this is the spread of the plasmid-encoded extended-spectrum b-lactamase (ESBL) genes, conferring resistance to third-generation cephalosporins. The purpose of this study was to investigate the molecular characteristics of ESBL-encoding genes identified in Escherichia coli cultured from diarrheic patients in China from 2013 to 2014. Materials and Methods: A total of 51 E. coli were confirmed as ESBL producers by double-disk synergy testing of 912 E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28242666/adjuvant-antibiotic-activity-of-acidic-sophorolipids-with-potential-for-facilitating-wound-healing
#11
Helen L Lydon, Niki Baccile, Breedge Callaghan, Roger Marchant, Christopher A Mitchell, Ibrahim M Banat
The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (>95% purity)...
February 27, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28239081/clinical-features-of-spontaneous-bacterial-peritonitis-a-10-year-experience-from-a-single-center
#12
Ha Young Na, Jeong Han Kim, Won Hyeok Choe, So Young Kwon, Byung Chul Yoo
Background/Aims: Spontaneous bacterial peritonitis (SBP) is one of critical complications in liver cirrhosis patients with ascites. We aimed to review clinical course of SBP patients in a 10-year period from single center. Methods: This study enrolled SBP patients between 2005 and 2015. Their medical records were reviewed. The laboratory findings of serum and ascites were examined, and characteristics of isolated microorganisms in ascites were analyzed. Results: Total 51 patients were enrolled...
February 25, 2017: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
https://www.readbyqxmd.com/read/28231673/-changes-of-resistant-phenotype-and-crispr-cas-system-of-four-shigella-strains-passaged-for-90-times-without-antibiotics
#13
B Zhang, L J Hong, G C Duan, W J Liang, H Y Yang, Y L Xi
Objective: To explore the stability of resistant phenotypes and changes of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) gene system on four Shigella strains in the absence of antibiotics. Methods: Four clinical isolated Shigella strains that resistant to different antibiotics were consecutive passaged for 90 times without antibiotics. Agar dilution method was used to determine the minimum inhibitory concentration of Shigella strains. After sequence analysis with PCR, CRISPR Finder and Clustal X 2...
February 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28224769/increasing-resistance-to-extended-spectrum-cephalosporins-fluoroquinolone-and-carbapenem-in-gram-negative-bacilli-and-the-emergence-of-carbapenem-non-susceptibility-in-klebsiella-pneumoniae-analysis-of-korean-antimicrobial-resistance-monitoring-system-karms
#14
Dokyun Kim, Ji Young Ahn, Chae Hoon Lee, Sook Jin Jang, Hyukmin Lee, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee
BACKGROUND: National surveillance of antimicrobial resistance becomes more important for the control of antimicrobial resistance and determination of treatment guidelines. We analyzed Korean Antimicrobial Resistance Monitoring System (KARMS) data collected from 2013 to 2015. METHODS: Of the KARMS participants, 16 secondary or tertiary hospitals consecutively reported antimicrobial resistance rates from 2013 to 2015. Data from duplicate isolates and institutions with fewer than 20 isolates were excluded...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28210466/antimicrobial-effects-of-ferula-gummosa-boiss-gum-against-extended-spectrum-%C3%AE-lactamase-producing-acinetobacter-clinical-isolates
#15
Fatemeh Farid Afshar, Parvaneh Saffarian, Hamideh Mahmoodzadeh Hosseini, Fereshteh Sattarian, Mohsen Amin, Abbas Ali Imani Fooladi
BACKGROUND AND OBJECTIVES: Acinetobacter spp. are important causes of nosocomial infections. They possess various antibiotic resistance mechanisms including extended spectrum beta lactamases (ESBLs). The aim of this study was to determine antibiotic resistance profile of Acinetobacter clinical isolates especially among ESBL-producing strains and to investigate the antimicrobial effects of oleo-gum-resin extract and essential oil of Ferula gummosa Boiss. MATERIALS AND METHODS: 120 Acinetobacter strains were isolated from various clinical samples of hospitalized patients in Baqiyatallah hospital, Tehran during 2011-2012...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28209468/characteristics-of-nontyphoidal-salmonella-gastroenteritis-in-taiwanese-children-a-9-year-period-retrospective-medical-record-review
#16
Yeh-Ting Hung, Chorng-Jang Lay, Chun-Lung Wang, Malcolm Koo
Nontyphoidal Salmonella is among the most common causes of acute pediatric gastroenteritis in Taiwan. It is important to study antibiotic resistance patterns in nontyphoidal Salmonella isolated from children. Therefore, the objective of this study was to investigate the prevalence of serogroups and susceptibility to antimicrobial nontyphoidal Salmonella isolated from Taiwanese children with gastroenteritis. A retrospective review of the medical records of patients aged under 18 years with a diagnosis of acute gastroenteritis at a regional hospital located in southern Taiwan from August 2000 to August 2009 was conducted...
February 13, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28194090/prevalence-and-characterization-of-salmonella-among-humans-in-ghana
#17
Linda Aurelia Andoh, Shabana Ahmed, John Elmerdahl Olsen, Kwasi Obiri-Danso, Mercy Jemima Newman, Japheth Awuletey Opintan, Lisa Barco, Anders Dalsgaard
BACKGROUND: Non-typhoidal Salmonella (NTS) is a public health problem worldwide and particularly in Africa with high disease burden. This study characterized Salmonella isolates from humans in Ghana to determine serovar distribution, phage types, and antimicrobial resistance. Further, the clonal relatedness among isolates was determined. METHODS: One hundred and thirty-seven Salmonella isolates (111 clinical and 26 public toilet) were characterized using standard serotyping, phage typing, and antimicrobial susceptibility testing methods...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/28192223/emergence-of-multi-drug-resistance-among-soil-bacteria-exposing-to-insecticides
#18
Kirubakaran Rangasamy, Murugan Athiappan, Natarajan Devarajan, Javid A Parray
Impacts of pesticide exposure on the soil microbial flora and cross resistance to antibiotics have not been well documented. Development of antibiotic resistance is a common issue among soil bacteria which are exposing to pesticides continuously at sub-lethal concentration. The present study was focused to evaluate the correlation between pesticide exposures and evolution of multi drug resistance among isolates collected from soil applied with insecticides. Twenty five insecticide (Monochrotophos) degrading bacteria were isolated from contaminated agricultural soil...
February 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28181665/multidrug-resistant-bacteria-among-patients-with-ventilatorassociated-pneumonia-in-an-emergency-intensive-care-unit-egypt
#19
Magda M Azzab, Rehab H El-Sokkary, Mohamed M Tawfeek, Manar G Gebriel
Ventilator-associated pneumonia (VAP) is the most common hospital-acquired infection among mechanically ventilated patients. Our objectives were to determine the incidence of VAP, isolate multidrug-resistant bacteria, identify the most prevalent resistant strains and identify their antibiotic susceptibility pattern. The VAP rate was calculated. The isolated microbes were identified and tested for antibiotic susceptibilities. The minimum inhibitory concentrations were determined of imipenem, meropenem and ertapenem for Klebsiella isolates...
February 1, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/28181300/synthesis-and-characterization-of-cefotaxime-conjugated-gold-nanoparticles-and-their-use-to-target-drug-resistant-ctx-m-producing-bacterial-pathogens
#20
Sibhghatulla Shaikh, Syed Mohd Danish Rizvi, Shazi Shakil, Talib Hussain, Thamir M Alshammari, Waseem Ahmad, Shams Tabrez, Mohammad H Al-Qahtani, Adel M Abuzenadah
Multidrug-resistance due to 'β lactamases having the expanded spectrum' (ESBLs) in members of Enterobacteriaceae is a matter of continued clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant bacterial strains (i.e. one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of 'cefotaxime loaded gold-nanoparticles'...
February 9, 2017: Journal of Cellular Biochemistry
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