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Yan Yan, Wen Ye, Qinkai Chen, Liu Yang, Li Zhang, Yu Liu, Xiaochen Zhou, Gongxian Wang
OBJECTIVE: To analyze and preliminarily validate the lncRNA expression profiles in the stenosis tissue of arteriovenous fistula (AVF). METHODS: A total of 54 uremic patients administered in the department of nephrology in the First Affiliated Hospital of Nanchang University between February 2017 and March 2017 were included in the study and subsequently categorized as experimental group, which included 12 patients with confirmed diagnosis of AVF, and control group which included 42 patients with no vascular stenosis...
April 12, 2018: Gene
Yan Zhang, Xiaoling Cheng, Hua Liang, Zhenzhen Jin
Homeobox (HOX) transcript antisense RNA (HOTAIR) is a long intergenic non-coding RNA (lncRNA) that has been reported to be highly upregulated in several types of cancers. However, the role of HOTAIR in human cervical cancer is still unclear. We therefore investigated the expression and probable function of HOTAIR in cervical cancer cells. The expression of HOTAIR was examined in (HeLa, CaSki, ME-180, HT-3) and Human Cervical Epithelial Cells (HCerEpiC) by qRT-PCR. Transfection of si-NC, si-HOTAIR or si-STAT3 was carried out with the help of Lipofectamine 2000...
March 20, 2018: Chemico-biological Interactions
Dan Yu, Wei-Min Wang, Fei-Fei Wu, Ping Ma, Ling Sun, Ding-Ming Wan, Yan-Fang Liu, Xin-Sheng Xie, Chong Wang, Hui Sun
OBJECTIVE: To investigate the expression of long-chain non-coding RNA RP11-87C12.5 in acute lymphocytic leukemia and its clinical significance. METHODS: LncRNA RP11-87C12.5 expression was detected by RT-PCR in bone marrow samples from 17 control group, 33 newly diagnosed ALL patients and 26 complete remission ALL patients after chemotherapy, at the same time the clinical data were collected and the clinical significance of IncRNA RP11-87C12.5 expression was analyzed...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yisheng Zhang, Hangyu Wu, Fuyan Wang, Mei Ye, Hong Zhu, Shizhong Bu
OBJECTIVE: To monitor the expression of long non-coding RNAs (lncRNAs) and changes in metabolic profiles among patients with gestational diabetes mellitus (GDM). METHODS: A retrospective case-control study was conducted using data for women treated at a hospital in China between October 8, 2014, and April 15, 2015. Patients diagnosed with GDM using an oral glucose tolerance test at 24-28 weeks of pregnancy (n=50) were included. The control group comprised pregnant women without GDM (n=47)...
February 2018: International Journal of Gynaecology and Obstetrics
Da-Ming Zhang, Zhao-Yu Lin, Zhao-Hui Yang, You-Yuan Wang, Di Wan, Jiang-Long Zhong, Pei-Lin Zhuang, Zhi-Quan Huang, Bin Zhou, Wei-Liang Chen
H19 is involved in tumor metastasis and associated with tumor progression. Enhancer of zest homolog 2 (EZH2) is overexpressed in multiple cancer types and correlates with tumor proliferation, epithelial-mesenchymal transition, and poor prognosis. However, the interaction between H19 and EZH2 to promote tongue squamous cell carcinoma (TSCC) progression remains largely uncharacterized. Insitu hybridization and quantitative reverse-transcription PCR (qRT-PCR) were performed to measure H19 expression in primary TSCC and adjacent normal tissues and cell lines...
2017: American Journal of Translational Research
Wenjie Sun, Yuhua Huang, Tieying Yin, Jingjing Wang, Ruolin Du, Juhui Qiu, Yuan Zhang, Yazhou Wang, Jinju Chen, Guixue Wang
Anticancer drugs are commonly used as inhibitors of vascular smooth muscle cell (VSMC) proliferation in clinical treatments. This study aims to investigate how elemene affects the proliferation of VSMCs, the restenosis, and the reendothelialization after implanting the elemene-coated stents. VSMCs derived from rat aorta were used to test the cell proliferation, cell cycle, migration, apoptosis, cytoskeletal protein F-actin, intracellular Ca(2+), IncRNA chip and gene expression of PCNA, P53, and Cx43 when cultured with elemene...
May 30, 2017: Biomaterials Science
Min Shi, Xiao-Yu Zhang, Heguo Yu, Shi-Hao Xiang, Ling Xu, Jue Wei, Qiong Wu, Rongrong Jia, Yu-Gang Wang, Xiao-Jie Lu
Hepatocellular Carcinoma (HCC) is one of the most fatal cancers, whose incidence and death rates are still rising. Here, we report the identification of long non-coding RNAs (IncRNAs) that associated with HCC progression and metabolism based on the systematically analysis of large scale RNA-seq data from HCC patients. We identified seven lncRNAs with high confidence which were highly related with prognostic of HCC. Of note, three of them had quite different expression patterns between the control samples and the patients, and their critical roles in cancer progression were validated...
July 4, 2017: Oncotarget
Chih-Kuan Chen, Chun-Ping Yu, Sung-Chou Li, Siao-Man Wu, Mei-Yeh Jade Lu, Yi-Hua Chen, Di-Rong Chen, Chen Siang Ng, Chau-Ti Ting, Wen-Hsiung Li
BACKGROUND: Long non-coding RNAs (lncRNAs) are important in various biological processes, but very few studies on lncRNA have been conducted in birds. To identify IncRNAs expressed during feather development, we analyzed single-stranded RNA-seq (ssRNA-seq) data from the anterior and posterior dorsal regions during zebra finch (Taeniopygia guttata) embryonic development. Using published transcriptomic data, we further analyzed the evolutionary conservation of IncRNAs in birds and amniotes...
January 31, 2017: BMC Genomics
Guang-Qing Huang, Zun-Ping Ke, Hai-Bo Hu, Biao Gu
Lung squamous cell carcinoma(LSCC) is the most common and aggressive lung tumor with poor clinical outcome. Previously studies showed that deregulation of long noncoding RNAs (lncRNAs) were involved in LSCC. We intended to figure out the role of lncRNAs in the regulation process of cancer-related genes and pathways they are involved. Data of 552 samples, including 501 cancer samples and 51 normal ones, were extracted from The Cancer Genome Atlas (TCGA). Differentially expressed lncRNAs (DEIs) were screened out (FDR<0...
February 2017: Cancer Biology & Therapy
Jie Wang, Bhaskar Roy
OBJECTIVE: To characterize transcriptome-wide lincRNAs of Hela-S3 cell line by analyzing RNA sequencing data to provide a foundation for further functional verification and clinical application of cervical carcinoma development. RESULTS: Single-cell RNA sequencing data of 37 Hela-S3 cells were analysed. On average, 511 lincRNAs were expressed in each cell. Comparing the expression difference of the lincRNAs and protein-coding genes, we found that lincRNAs expression displayed more cell specificity than that of protein-coding genes (t-test, P<2...
March 2017: Biotechnology Letters
(no author information available yet)
No abstract text is available yet for this article.
November 12, 2016: Chinese Journal of Tuberculosis and Respiratory Diseases
Li Wang, Zhenhong Chen, Li An, Yajuan Wang, Zhijian Zhang, Yinghua Guo, Changting Liu
BACKGROUND/AIMS: Long non-coding RNAs (lncRNAs) play an important role in tumorigenesis. However, the role of lncRNA expression in human Non-small cell lung cancer (NSCLC) biology, prognosis and molecular classification remains unknown. METHODS: We established the IncRNA profile in NSCLC by re-annotation of microarrays from the Gene expression omnibus database. Quantitative real-time PCR was used to determine expression of LINC00342. RESULTS: 6066 differentially expressed IncRNAs were identified and we found a novel IncRNA, LINC00342 was significantly up-regulated in NSCLC tissues compared with normal tissues...
2016: Cellular Physiology and Biochemistry
O I Kit, E Yu Kirichenko, Yu G Kirichenko, I A Novikova, O N Selyutina, S Yu Filippova
The last decade is characterized by development of such technologies as RNA-sequencing and biochips which resulted in discovery of new perspective biomarkersfor personalized diagnostic of cancer. Among them, the long non-coding RNA (IncRNA) are of special interest because according the recent studies they are positioned as important regulators of gene expression on epigenetic, transcription and post-transcription levels. The review considers the role of long non-coding RNA in cancerogenesis. The corresponding of their application in diagnostic is evaluated...
January 2016: Klinicheskaia Laboratornaia Diagnostika
Dan Li, Lei Song, Zhongmei Wen, Xiaoping Li, Jing Jie, Yan Wang, Liping Peng
Long noncoding RNAs (IncRNAs), involved in cancer biology, contributing to essential cancer cell functions such as proliferation, invasion, and metastasis, have received increasing attention recently. Human Zinc ribbon domain containing 1 (ZNRD1) has been confirmed to be involved in carcinogenesis and development of multiple cancers. ZNRD1-AS1, a lncRNA in the upstream region of ZNRD1 which could down-regulate the expression of ZNRD1, has been identified as a possible component in carcinogenesis. The underlying relations of ZNRD1-AS1 with lung cancer development and metastasis remain obscure...
June 14, 2016: Oncotarget
Yingying Shi, Huilin Tu, Xiong Chen, Yingying Zhang, Liujun Chen, Zhongchun Liu, Jiqun Sheng, Song Han, Jun Yin, Biwen Peng, Xiaohua He, Wanhong Liu
Coxsackievirus A16 (CVA16) is one of major pathogens of hand, foot and mouth disease (HFMD) in children. Long non-coding RNAs (IncRNAs) have been implicated in various biological processes, but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of IncRNAs of normal and CVA16 infected rhabdomyosarcoma (RD) cells using RNA-Seq to investigate the functional relevance of IncRNAs. We showed that a total of 760 IncRNAs were upregulated and 1210 IncRNAs were downregulated...
April 2016: Virologica Sinica
M Buchholz
The development of malignant tumors from healthy tissues is associated with profound changes in expression profiles of a large number of mRNAs, miRNAs and IncRNAs. These changes on the one hand permit insights into the biology of individual tumors; on the other hand, tumor-derived RNAs can also be detected in circulating blood and serve as specific markers for differential diagnosis and patient prognosis.
January 2016: Die Pharmazie
O Rossbach, A Bindereif
Non-coding RNAs (ncRNAs) in eukaryotes have recently developed to a very active research area in RNA biology, opening up new strategies for diagnosis and therapies of human disease. Here we introduce and describe the most important classes of eukaryotic ncRNAs: microRNAs (miRNAs), long non-coding RNAs (IncRNAs), and circular RNAs (circRNAs). We further discuss new RNA-based diagnostic and therapeutic concepts.
January 2016: Die Pharmazie
Susanne Voelter-Mahlknecht
Cardiovascular diseases (CVD) increasingly burden societies with vast financial and health care problems. Therefore, the importance of improving preventive and therapeutic measures against cardiovascular diseases is continually growing. To accomplish such improvements, research must focus particularly on understanding the underlying mechanisms of such diseases, as in the field of epigenetics, and pay more attention to strengthening primary prevention. To date, preliminary research has found a connection between DNA methylation, histone modifications, RNA-based mechanisms and the development of CVD like atherosclerosis, cardiac hypertrophy, myocardial infarction, and heart failure...
2016: Clinical Epigenetics
Yi Zhu, Ri-kao Yu, A-fin Ji, Xiao-lin Yao, Jia-jie Fang, Xiao-dong Jin
OBJECTIVE: To investigate the expression of long non-coding RNA-HOTAIR in prostate cancer cells and its effects on the growth and metastasis of the cells. METHODS: Using quantitative reverse-transcription PCR (qRT-PCR), we determined the relative expression of HOTAIR in the normal human prostate epithelial cell line RWPE-I and prostate cancer cell lines PC-3 and DU145. We detected the effects of HOTAIR on the cell cycle and invasiveness of prostate cancer cells by RNA interference, flow cytometry, and Transwell mitration assay...
September 2015: Zhonghua Nan Ke Xue, National Journal of Andrology
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