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Chih-Kuan Chen, Chun-Ping Yu, Sung-Chou Li, Siao-Man Wu, Mei-Yeh Jade Lu, Yi-Hua Chen, Di-Rong Chen, Chen Siang Ng, Chau-Ti Ting, Wen-Hsiung Li
BACKGROUND: Long non-coding RNAs (lncRNAs) are important in various biological processes, but very few studies on lncRNA have been conducted in birds. To identify IncRNAs expressed during feather development, we analyzed single-stranded RNA-seq (ssRNA-seq) data from the anterior and posterior dorsal regions during zebra finch (Taeniopygia guttata) embryonic development. Using published transcriptomic data, we further analyzed the evolutionary conservation of IncRNAs in birds and amniotes...
January 31, 2017: BMC Genomics
Guang-Qing Huang, Zun-Ping Ke, Hai-Bo Hu, Biao Gu
Lung squamous cell carcinoma(LSCC) is the most common and aggressive lung tumor with poor clinical outcome. Previously studies showed that deregulation of long noncoding RNAs (lncRNAs) were involved in LSCC. We intended to figure out the role of lncRNAs in the regulation process of cancer-related genes and pathways they are involved. Data of 552 samples, including 501 cancer samples and 51 normal ones, were extracted from The Cancer Genome Atlas (TCGA). Differentially expressed lncRNAs (DEIs) were screened out (FDR<0...
February 2017: Cancer Biology & Therapy
Jie Wang, Bhaskar Roy
OBJECTIVE: To characterize transcriptome-wide lincRNAs of Hela-S3 cell line by analyzing RNA sequencing data to provide a foundation for further functional verification and clinical application of cervical carcinoma development. RESULTS: Single-cell RNA sequencing data of 37 Hela-S3 cells were analysed. On average, 511 lincRNAs were expressed in each cell. Comparing the expression difference of the lincRNAs and protein-coding genes, we found that lincRNAs expression displayed more cell specificity than that of protein-coding genes (t-test, P<2...
March 2017: Biotechnology Letters
(no author information available yet)
No abstract text is available yet for this article.
November 12, 2016: Chinese Journal of Tuberculosis and Respiratory Diseases
Li Wang, Zhenhong Chen, Li An, Yajuan Wang, Zhijian Zhang, Yinghua Guo, Changting Liu
BACKGROUND/AIMS: Long non-coding RNAs (lncRNAs) play an important role in tumorigenesis. However, the role of lncRNA expression in human Non-small cell lung cancer (NSCLC) biology, prognosis and molecular classification remains unknown. METHODS: We established the IncRNA profile in NSCLC by re-annotation of microarrays from the Gene expression omnibus database. Quantitative real-time PCR was used to determine expression of LINC00342. RESULTS: 6066 differentially expressed IncRNAs were identified and we found a novel IncRNA, LINC00342 was significantly up-regulated in NSCLC tissues compared with normal tissues...
2016: Cellular Physiology and Biochemistry
O I Kit, E Yu Kirichenko, Yu G Kirichenko, I A Novikova, O N Selyutina, S Yu Filippova
The last decade is characterized by development of such technologies as RNA-sequencing and biochips which resulted in discovery of new perspective biomarkersfor personalized diagnostic of cancer. Among them, the long non-coding RNA (IncRNA) are of special interest because according the recent studies they are positioned as important regulators of gene expression on epigenetic, transcription and post-transcription levels. The review considers the role of long non-coding RNA in cancerogenesis. The corresponding of their application in diagnostic is evaluated...
January 2016: Klinicheskaia Laboratornaia Diagnostika
Dan Li, Lei Song, Zhongmei Wen, Xiaoping Li, Jing Jie, Yan Wang, Liping Peng
Long noncoding RNAs (IncRNAs), involved in cancer biology, contributing to essential cancer cell functions such as proliferation, invasion, and metastasis, have received increasing attention recently. Human Zinc ribbon domain containing 1 (ZNRD1) has been confirmed to be involved in carcinogenesis and development of multiple cancers. ZNRD1-AS1, a lncRNA in the upstream region of ZNRD1 which could down-regulate the expression of ZNRD1, has been identified as a possible component in carcinogenesis. The underlying relations of ZNRD1-AS1 with lung cancer development and metastasis remain obscure...
June 14, 2016: Oncotarget
Yingying Shi, Huilin Tu, Xiong Chen, Yingying Zhang, Liujun Chen, Zhongchun Liu, Jiqun Sheng, Song Han, Jun Yin, Biwen Peng, Xiaohua He, Wanhong Liu
Coxsackievirus A16 (CVA16) is one of major pathogens of hand, foot and mouth disease (HFMD) in children. Long non-coding RNAs (IncRNAs) have been implicated in various biological processes, but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of IncRNAs of normal and CVA16 infected rhabdomyosarcoma (RD) cells using RNA-Seq to investigate the functional relevance of IncRNAs. We showed that a total of 760 IncRNAs were upregulated and 1210 IncRNAs were downregulated...
April 2016: Virologica Sinica
M Buchholz
The development of malignant tumors from healthy tissues is associated with profound changes in expression profiles of a large number of mRNAs, miRNAs and IncRNAs. These changes on the one hand permit insights into the biology of individual tumors; on the other hand, tumor-derived RNAs can also be detected in circulating blood and serve as specific markers for differential diagnosis and patient prognosis.
January 2016: Die Pharmazie
O Rossbach, A Bindereif
Non-coding RNAs (ncRNAs) in eukaryotes have recently developed to a very active research area in RNA biology, opening up new strategies for diagnosis and therapies of human disease. Here we introduce and describe the most important classes of eukaryotic ncRNAs: microRNAs (miRNAs), long non-coding RNAs (IncRNAs), and circular RNAs (circRNAs). We further discuss new RNA-based diagnostic and therapeutic concepts.
January 2016: Die Pharmazie
Susanne Voelter-Mahlknecht
Cardiovascular diseases (CVD) increasingly burden societies with vast financial and health care problems. Therefore, the importance of improving preventive and therapeutic measures against cardiovascular diseases is continually growing. To accomplish such improvements, research must focus particularly on understanding the underlying mechanisms of such diseases, as in the field of epigenetics, and pay more attention to strengthening primary prevention. To date, preliminary research has found a connection between DNA methylation, histone modifications, RNA-based mechanisms and the development of CVD like atherosclerosis, cardiac hypertrophy, myocardial infarction, and heart failure...
2016: Clinical Epigenetics
Yi Zhu, Ri-kao Yu, A-fin Ji, Xiao-lin Yao, Jia-jie Fang, Xiao-dong Jin
OBJECTIVE: To investigate the expression of long non-coding RNA-HOTAIR in prostate cancer cells and its effects on the growth and metastasis of the cells. METHODS: Using quantitative reverse-transcription PCR (qRT-PCR), we determined the relative expression of HOTAIR in the normal human prostate epithelial cell line RWPE-I and prostate cancer cell lines PC-3 and DU145. We detected the effects of HOTAIR on the cell cycle and invasiveness of prostate cancer cells by RNA interference, flow cytometry, and Transwell mitration assay...
September 2015: Zhonghua Nan Ke Xue, National Journal of Andrology
Yanni Lyu, Yisong Qian, Longsheng Fu
OBJECTIVE: To predict the regulating network of innate immunity signaling molecule hsa-miR-181a in stroke based on the methods of bioinformatics. METHODS: The UCSC genome browser, the human miRNA disease database (HMDD), the transcription factor-miRNA regulation database (TransmiR), the database on predicted and validated miRNA targets (miRwalk), the Genecards, the long non-coding RNA (LncRNA) disease database, the DIANA LAB-LncBase and the ConSite were employed to study the upstream transcription factor, downstream target genes and the interactive LncRNA of hsa-miR-181a and to draw the core regulating network of hsa-miR-181a...
August 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Zhenyu Ye, Ming Zhou, Bin Tian, Bin Wu, Juncheng Li
OBJECTIVE: To explore the expression and clinical significance of IncRNA-CCAT and EMT related molecule E-cadherin and N-cadherin in colorectal cancer. METHODS: The expression of IncRNA-CCAT1, E-cadherin and N-cadherin in 37 colorectal cancer tissue and para-carcinoma tissue was detected using qRT-PCR method, and the correlation of expression level with clinical and pathological features was studied. RESULTS: The expression of IncRNA-CCAT1 in tumor tissue was significantly higher than that in normal para-carcinoma tissue (P < 0...
2015: International Journal of Clinical and Experimental Medicine
Qing He, Jinyun Tan, Bo Yu, Weihao Shi, Kun Liang
Long non-coding RNAs (IncRNAs) play important roles in various biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little is known about the role of IncRNA HIF 1 alpha-antisense RNA 1 (HIF1a-AS1) in regulating the proliferation and apoptosis of vascular smooth muscle cells (VSMCs) and the expression of HIF1a-AS1 in serum of thoracoabdominal aortic aneurysm (TAAA) patients. The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining...
May 2015: Die Pharmazie
Dan-dan Yin, Er-bao Zhang, Liang-hui You, Ning Wang, Lin-tao Wang, Fei-yan Jin, Ya-nan Zhu, Li-hua Cao, Qing-xin Yuan, Wei De, Wei Tang
BACKGROUND: Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic β cell functioning both in vitro and in vivo. METHODS: qRT-PCR analyses were performed to detect the expression of lncRNA TUG1 in different tissues. RNAi, MTT, TUNEL and Annexin V-FITC assays and western blot, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on cell apoptosis and insulin secretion in vitro and in vivo...
2015: Cellular Physiology and Biochemistry
Yan-fen Zou, Li-zhou Sun
OBJECTIVE: To investigate the expression of long noncoding RNA (lncRNA) HOTAIR in pre-eclampsia (PE) placentas and its effect on trophoblast cells proliferation, invasion, and apoptosis. METHODS: The expression of HOTAIR was determined by qPCR for 23 severe PE and 23 normal pregnant women. qRT-PCR was used to detect the efficiency of overexpression and knock-down after the HTR-8/SVneo cells were transfected with HOTAIR overexpression and siRNAs targeting HOTAIR for 24-48 h, respectively...
January 2015: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
Chang-Min Lin, Yang Liu, Keng Huang, Xian-Cai Chen, Bo-Zhi Cai, Hai-Hong Li, Yan-Ping Yuan, Huan Zhang, Yu Li
Dermal papilla (DP) cells may be the source of dermal-derived signaling molecules involved in hair-follicle development and postnatal hair cycling. Early-passage DP cells can induce hair growth in vivo, but, on further culture, this ability is lost. The cellular mechanisms underlying the hair-follicle induction property of early-passage DP cells are unclear. Long noncoding RNAs (lncRNAs) are an important class of genes involved in various biological functions. They are aberrantly expressed and play roles in the regulation of the Wnt signaling pathway, a critical point in maintaining hair-induction activity...
October 24, 2014: Biochemical and Biophysical Research Communications
Qi-Rui Wang, Hao-Ning Fan, Zhi-Xin Yin, Hong-Bing Cai, Meng Shao, Jian-Xin Diao, Yuan-Liang Liu, Xue-Gang Sun, Li Tong, Qin Fan
OBJECTIVE: To investigate the effect of curcumin (Cur) on radiosensitivity of nasopharyngeal carcinoma cell CNE-2 and its mechanism. METHOD: The effect of curcumin on radiosensitivity was determined by the clone formation assay. The cell survival curve was fitted by Graph prism 6. 0. The changes in cell cycle were analyzed by flow cytometry (FCM). The differential expression of long non-coding RNA was detected by gene chip technology. Part of differentially expressed genes was verified by Real-time PCR...
February 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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