Ann-Marie Baker, Gayathri Nageswaran, Pablo Nenclares, Tahel Ronel, Kane Smith, Christopher Kimberley, Miangela M Laclé, Shreerang Bhide, Kevin J Harrington, Alan Melcher, Manuel Rodriguez-Justo, Benny Chain, Trevor A Graham
Genomic analysis of the T-cell receptor (TCR) reveals the strength, breadth, and clonal dynamics of the adaptive immune response to pathogens or cancer. The diversity of the TCR repertoire, however, means that sequencing is technically challenging, particularly for samples with low quality, degraded nucleic acids. Here, we developed and validated FUME-TCRseq, a robust and sensitive RNA-based TCR sequencing methodology that is suitable for formalin-fixed paraffin-embedded samples and low amounts of input material...
March 14, 2024: Cancer Research