keyword
MENU ▼
Read by QxMD icon Read
search

Microbial adherence

keyword
https://www.readbyqxmd.com/read/28301290/production-of-a-halotolerant-biofilm-from-green-coffee-beans-immobilized-on-loofah-fiber-luffa-cylindrica-and-its-effect-on-phenanthrene-degradation-in-seawater
#1
Sonia Acosta-Rubí, Araceli Tomasini Campocosio, María Del Carmen Montes-Horcasitas, Liliana Quintanar-Vera, Fernando Esparza-García, Refugio Rodríguez-Vázquez
A biofilm developed from low quality green coffee beans was tested for its capacity to degrade the polynuclear aromatic hydrocarbon (PAH), phenanthrene (Phe), in seawater. Microorganisms were immobilized on two types of Luffa cylindrica (with three and four placental cavities), and the effects of moisture content (20, 30 and 40% of water holding capacity) and particle size (<0.42 mm, 0.42-0.86 mm and 0.86-2.0 mm) of green coffee beans on microbial activity were considered. Biofilm growth determined by respirometry showed a highest microbial activity at a moisture content of 40% and particle size of 0...
March 16, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28295372/surface-charge-modification-decreases-pseudomonas-aeruginosa-adherence-in-vitro-and-bacterial-persistence-in-an-in-vivo-implant-model
#2
W Katherine Kao, Patricia M Gagnon, Joseph P Vogel, Richard A Chole
OBJECTIVE: Chronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. These infections are thought to be due to the establishment of microbial biofilms on implant surfaces. To address this issue, we hypothesized that surface charge modification may inhibit the formation of Pseudomonas aeruginosa biofilms on implant surfaces in vitro and in vivo. STUDY DESIGN: We evaluated the effect of surface charge modification on bacterial biofilm formation by assessing the effect of the surface charge on bacterial adhesion in vitro and bacterial persistence in vivo...
March 14, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28246035/functional-gene-profiling-through-metarnaseq-approach-reveals-diet-dependent-variation-in-rumen-microbiota-of-buffalo-bubalus-bubalis
#3
Ankit T Hinsu, Nidhi R Parmar, Neelam M Nathani, Ramesh J Pandit, Anand B Patel, Amrutlal K Patel, Chaitanya G Joshi
Recent advances in next generation sequencing technology have enabled analysis of complex microbial community from genome to transcriptome level. In the present study, metatranscriptomic approach was applied to elucidate functionally active bacteria and their biological processes in rumen of buffalo (Bubalus bubalis) adapted to different dietary treatments. Buffaloes were adapted to a diet containing 50:50, 75:25 and 100:0 forage to concentrate ratio, each for 6 weeks, before ruminal content sample collection...
February 27, 2017: Anaerobe
https://www.readbyqxmd.com/read/28240279/specialized-activities-and-expression-differences-for-clostridium-thermocellum-biofilm-and-planktonic-cells
#4
Alexandru Dumitrache, Dawn M Klingeman, Jace Natzke, Miguel Rodriguez, Richard J Giannone, Robert L Hettich, Brian H Davison, Steven D Brown
Clostridium (Ruminiclostridium) thermocellum is a model organism for its ability to deconstruct plant biomass and convert the cellulose into ethanol. The bacterium forms biofilms adherent to lignocellulosic feedstocks in a continuous cell-monolayer in order to efficiently break down and uptake cellulose hydrolysates. We developed a novel bioreactor design to generate separate sessile and planktonic cell populations for omics studies. Sessile cells had significantly greater expression of genes involved in catabolism of carbohydrates by glycolysis and pyruvate fermentation, ATP generation by proton gradient, the anabolism of proteins and lipids and cellular functions critical for cell division consistent with substrate replete conditions...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229593/development-of-laser-structured-liquid-infused-titanium-with-strong-biofilm-repellent-properties
#5
Katharina Doll, Elena Fadeeva, Joern Schaeske, Tobias Ehmke, Andreas Winkel, Alexander Heisterkamp, Boris N Chichkov, Meike Stiesch, Nico S Stumpp
Medical implants are commonly used in modern medicine but still harbor the risk of microbial infections caused by bacterial biofilms. As their retrospective treatment is difficult, there is a need for biomedical materials, which inhibit bacterial colonization from the start without using antibacterial agents, as these can promote resistance development. The promising concept of slippery liquid-infused porous surfaces (SLIPS) possesses enormous potential for this purpose. In the present study, this principle was applied to titanium, a common material in implantology, and its biofilm-repellent properties were demonstrated...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28221885/antibacterial-and-antiadhesive-activities-of-extracts-from-edible-plants-against-soft-drink-spoilage-by-asaia-spp
#6
Hubert Antolak, Agata Czyzowska, Dorota Kregiel
This study was conducted to investigate the antibacterial and antiadhesive activities of ethanol extracts from five edible plant parts: cinnamon bark ( Cinnamomum zeylanicum ), licorice root ( Glycyrrhiza radix ), nettle leaves ( Urtica dioica ), green tea leaves ( Camellia sinensis ), and elderberry flowers ( Sambucus nigra ). The chemical constituents of the extracts were identified using high-performance liquid chromatography and liquid chromatography plus mass spectrometry. Six strains of Asaia lannensis and Asaia bogorensis bacteria isolated from spoiled commercial fruit-flavored noncarbonated mineral water were used...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28207869/impact-of-wall-shear-stress-on-initial-bacterial-adhesion-in-rotating-annular-reactor
#7
Thibaut Saur, Emilie Morin, Frédéric Habouzit, Nicolas Bernet, Renaud Escudié
The objective of this study was to investigate the bacterial adhesion under different wall shear stresses in turbulent flow and using a diverse bacterial consortium. A better understanding of the mechanisms governing microbial adhesion can be useful in diverse domains such as industrial processes, medical fields or environmental biotechnologies. The impact of wall shear stress-four values ranging from 0.09 to 7.3 Pa on polypropylene (PP) and polyvinyl chloride (PVC)-was carried out in rotating annular reactors to evaluate the adhesion in terms of morphological and microbiological structures...
2017: PloS One
https://www.readbyqxmd.com/read/28193634/purified-streptococcus-pneumoniae-endopeptidase-o-pepo-enhances-particle-uptake-by-macrophages-in-a-tlr2-and-mir-155-dependent-manner
#8
Hua Yao, Hong Zhang, Kai Lan, Hong Wang, Yufeng Su, Dagen Li, Zhixin Song, Fang Cui, Yibing Yin, Xuemei Zhang
Insights into the host-microbial virulence factors interaction, especially the immune-signaling mechanisms, could provide novel prevention and treatment options for pneumococcal diseases. Streptococcus pneumoniae endopeptidase O (PepO) is a newly discovered and ubiquitously expressed pneumococcal virulence protein. A PepO-mutant strain showed impaired adherence to and invasion of host cells compared with their isogenic wild-type strains. It is still unknown whether PepO is involved in host defense response against pneumococcal infection...
February 13, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28179509/iga-coated-e-coli-enriched-in-crohn-s-disease-spondyloarthritis-promote-th17-dependent-inflammation
#9
Monica Viladomiu, Charles Kivolowitz, Ahmed Abdulhamid, Belgin Dogan, Daniel Victorio, Jim G Castellanos, Viola Woo, Fei Teng, Nhan L Tran, Andrew Sczesnak, Christina Chai, Myunghoo Kim, Gretchen E Diehl, Nadim J Ajami, Joseph F Petrosino, Xi K Zhou, Sergio Schwartzman, Lisa A Mandl, Meira Abramowitz, Vinita Jacob, Brian Bosworth, Adam Steinlauf, Ellen J Scherl, Hsin-Jung Joyce Wu, Kenneth W Simpson, Randy S Longman
Peripheral spondyloarthritis (SpA) is a common extraintestinal manifestation in patients with active inflammatory bowel disease (IBD) characterized by inflammatory enthesitis, dactylitis, or synovitis of nonaxial joints. However, a mechanistic understanding of the link between intestinal inflammation and SpA has yet to emerge. We evaluated and functionally characterized the fecal microbiome of IBD patients with or without peripheral SpA. Coupling the sorting of immunoglobulin A (IgA)-coated microbiota with 16S ribosomal RNA-based analysis (IgA-seq) revealed a selective enrichment in IgA-coated Escherichia coli in patients with Crohn's disease-associated SpA (CD-SpA) compared to CD alone...
February 8, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28178315/composition-and-pathogenic-potential-of-a-microbial-bioremediation-product-used-for-crude-oil-degradation
#10
Azam F Tayabali, Gordon Coleman, Jennifer Crosthwait, Kathy C Nguyen, Yan Zhang, Philip Shwed
A microbial bioremediation product (MBP) used for large-scale oil degradation was investigated for microbial constituents and possible pathogenicity. Aerobic growth on various media yielded >108 colonies mL-1. Full-length 16S rDNA sequencing and fatty acid profiling from morphologically distinct colonies revealed ≥13 distinct genera. Full-length 16S rDNA library sequencing, by either Sanger or long-read PacBio technology, suggested that up to 21% of the MBP was composed of Arcobacter. Other high abundance microbial constituents (>6%) included the genera Proteus, Enterococcus, Dysgonomonas and several genera in the order Bacteroidales...
2017: PloS One
https://www.readbyqxmd.com/read/28143978/the-type-iva-pilus-machinery-is-recruited-to-sites-of-future-cell-division
#11
Tyson Carter, Ryan N C Buensuceso, Stephanie Tammam, Ryan P Lamers, Hanjeong Harvey, P Lynne Howell, Lori L Burrows
Type IVa pili (T4aP) are ubiquitous microbial appendages used for adherence, twitching motility, DNA uptake, and electron transfer. Many of these functions depend on dynamic assembly and disassembly of the pilus by a megadalton-sized, cell envelope-spanning protein complex located at the poles of rod-shaped bacteria. How the T4aP assembly complex becomes integrated into the cell envelope in the absence of dedicated peptidoglycan (PG) hydrolases is unknown. After ruling out the potential involvement of housekeeping PG hydrolases in the installation of the T4aP machinery in Pseudomonas aeruginosa, we discovered that key components of inner (PilMNOP) and outer (PilQ) membrane subcomplexes are recruited to future sites of cell division...
January 31, 2017: MBio
https://www.readbyqxmd.com/read/28112955/fate-of-eight-different-polymers-under-uncontrolled-composting-conditions-relationships-between-deterioration-biofilm-formation-and-the-material-surface-properties
#12
Anne Mercier, Kevin Gravouil, Willy Aucher, Sandra Brosset-Vincent, Linette Kadri, Jenny Colas, Didier Bouchon, Thierry Ferreira
With the ever-increasing volume of polymer wastes and their associated detrimental impacts on the environment, the plastic life cycle has drawn increasing attention. Here, eight commercial polymers selected from biodegradable to environmentally persistent materials, all formulated under a credit card format, were incubated in an outdoor compost to evaluate their fate over time and to profile the microbial communities colonizing their surfaces. After 450 days in compost, the samples were all colonized by multispecies biofilms, these latest displaying different amounts of adhered microbial biomass and significantly distinct bacterial and fungal community compositions depending on the substrate...
February 8, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28097056/rhea-a-transparent-and-modular-r-pipeline-for-microbial-profiling-based-on-16s-rrna-gene-amplicons
#13
Ilias Lagkouvardos, Sandra Fischer, Neeraj Kumar, Thomas Clavel
The importance of 16S rRNA gene amplicon profiles for understanding the influence of microbes in a variety of environments coupled with the steep reduction in sequencing costs led to a surge of microbial sequencing projects. The expanding crowd of scientists and clinicians wanting to make use of sequencing datasets can choose among a range of multipurpose software platforms, the use of which can be intimidating for non-expert users. Among available pipeline options for high-throughput 16S rRNA gene analysis, the R programming language and software environment for statistical computing stands out for its power and increased flexibility, and the possibility to adhere to most recent best practices and to adjust to individual project needs...
2017: PeerJ
https://www.readbyqxmd.com/read/28076690/effect-of-intermittent-aeration-strategies-on-treatment-performance-and-microbial-community-of-an-ifas-reactor-treating-municipal-waste-water
#14
Nitin Kumar Singh, Akansha Bhatia, Absar Ahmad Kazmi
This study investigated the effect of various intermittent aeration (IA) cycles on organics and nutrient removal, and microbial communities in an integrated fixed-film activated sludge (IFAS) reactor treating municipal waste water. Average effluent biological oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids, total nitrogen (TN) and total phosphorus (TP) values were noted as 20, 50, 30, 12 and 1.5 mgL(-1), respectively, in continuous aeration mode. A total of four operational conditions (run 1, continuous aeration; run 2, 150/30 min aeration on/off time; run 3, 120/60 min aeration on/off time and run 4, 90/60 min aeration on/off time) were investigated in IFAS reactor assessment...
January 31, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28029051/adherence-to-a-mediterranean-diet-influences-the-fecal-metabolic-profile-of-microbial-derived-phenolics-in-a-spanish-cohort-of-middle-age-and-older-people
#15
Isabel Gutiérrez-Díaz, Tania Fernández-Navarro, Nuria Salazar, Begoña Bartolomé, M Victoria Moreno-Arribas, Enrique Juan de Andres-Galiana, Juan Luis Fernández-Martínez, Clara G de Los Reyes-Gavilán, Miguel Gueimonde, Sonia González
Despite the evidence regarding the influence of certain polyphenol food sources on the metabolic profile in feces, the association between the different phenolics provided by the diet and the fecal phenolic profile has not been elucidated. In this study, the composition of phenolic metabolites in fecal solutions was analyzed by UPLC-ESI-MS/MS in 74 volunteers. This fecal phenolic profile showed a high interindividual variation of the different compounds analyzed, phenylacetic and phenylpropionic acids being the major classes of phenolic metabolites excreted in feces...
January 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28011597/exploring-the-methanogen-and-bacterial-communities-of-rumen-environments-solid-adherent-fluid-and-epimural
#16
Thijs De Mulder, Karen Goossens, Nico Peiren, Leen Vandaele, Annelies Haegeman, Caroline De Tender, Tom Ruttink, Tom Van de Wiele, Sam De Campeneere
The rumen microbiome occupies a central role in animal health and productivity. A better understanding of the rumen ecosystem is essential to increase productivity or decrease methane production. Samples were collected from the three main rumen environments: the solid adherent fraction, the liquid fraction and the epithelium. For the liquid and solid fraction, two alternative sample processing protocols were compared, resulting in a total of five sample types: crude solids (S), the eluted solid adherent fraction (Ad), free-living species in the crude rumen liquid (CRL), strained liquid samples (Lq) and epimural scrapings (Ep)...
December 22, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27997146/silver-sink-effect-of-humic-acid-on-bacterial-surface-colonization-in-the-presence-of-silver-ions-and-nanoparticles
#17
Alex J Bertuccio, Robert D Tilton
Silver nanoparticles (AgNPs) released from consumer products may enter the environment and possibly harm microbial communities. Prior research showed that surface-adherent AgNPs inhibit bacterial surface colonization, a precursor to biofilm formation, only when planktonic bacterial inoculum concentrations are less than a threshold level ( Wirth and co-workers, J. Colloid Interface Sci. 2016 , 467 , 17 - 27 ). This inoculum effect is due to a decrease in free silver ion concentration associated with sublethal binding to bacteria...
January 9, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27996032/targeting-bacterial-adherence-inhibits-multidrug-resistant-pseudomonas-aeruginosa-infection-following-burn-injury
#18
Ryan M Huebinger, Daniel H Stones, Marcela de Souza Santos, Deborah L Carlson, Juquan Song, Diana Pereira Vaz, Emma Keen, Steven E Wolf, Kim Orth, Anne Marie Krachler
Classical antimicrobial drugs target proliferation and therefore place microbes under extreme selective pressure to evolve resistance. Alternative drugs that target bacterial virulence without impacting survival directly offer an attractive solution to this problem, but to date few such molecules have been discovered. We previously discovered a widespread group of bacterial adhesins, termed Multivalent Adhesion Molecules (MAMs) that are essential for initial binding of bacteria to host tissues and virulence...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27983723/viruses-and-the-origin-of-microbiome-selection-and-immunity
#19
Steven D Quistad, Juris A Grasis, Jeremy J Barr, Forest L Rohwer
The last common metazoan ancestor (LCMA) emerged over half a billion years ago. These complex metazoans provided newly available niche space for viruses and microbes. Modern day contemporaries, such as cnidarians, suggest that the LCMA consisted of two cell layers: a basal endoderm and a mucus-secreting ectoderm, which formed a surface mucus layer (SML). Here we propose a model for the origin of metazoan immunity based on external and internal microbial selection mechanisms. In this model, the SML concentrated bacteria and their associated viruses (phage) through physical dynamics (that is, the slower flow fields near a diffusive boundary layer), which selected for mucin-binding capabilities...
April 2017: ISME Journal
https://www.readbyqxmd.com/read/27981504/isolation-identification-and-screening-of-potential-probiotic-bacteria-in-milk-from-south-african-saanen-goats
#20
Goitsemang Makete, Olayinka A Aiyegoro, Mapitsi S Thantsha
This study aimed to evaluate lactic acid bacteria isolates from Saanen goats' milk for probiotic attributes, thereby determining their potential as direct-fed microbials for goats. Isolates were identified using API 50CH system, 16S rDNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. All 17 isolates obtained were identified as Lactobacillus plantarum except one identified as Pediococcus acidilactici. Four isolates identified as L. plantarum (Accession numbers KJ026587...
December 15, 2016: Probiotics and Antimicrobial Proteins
keyword
keyword
35769
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"