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Maldi-tof identification

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https://www.readbyqxmd.com/read/28432276/identification-of-common-immunodominant-antigens-of-eimeria-tenella-eimeria-acervulina-and-eimeria-maxima-by-immunoproteomic-analysis
#1
Lianrui Liu, Xinmei Huang, Jianhua Liu, Wenyu Li, Yihong Ji, Di Tian, Lu Tian, Xinchao Yang, Lixin Xu, Ruofeng Yan, Xiangrui Li, Xiaokai Song
Clinical chicken coccidiosis is mostly caused by simultaneous infection of several Eimeria species, and host immunity against Eimeria is species-specific. It is urgent to identify common immunodominant antigen of Eimeria for developing multivalent anticoccidial vaccines. In this study, sporozoite proteins of Eimeria tenella, Eimeria acervulina and Eimeria maxima were analyzed by two-dimensional electrophoresis (2DE). Western bot analysis was performed on the yielded 2DE gel using antisera of E. tenella E. acervulina and E...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28431114/molecular-characterization-of-cryptococcus-neoformans-isolated-from-the-environment-in-beijing-china
#2
Hongtao Dou, Huizhu Wang, Shaowei Xie, Xinxin Chen, Zhipeng Xu, Yingchun Xu
The molecular type of environmental Cryptococcus neoformans in Beijing was not clear. Our study aims to reveal the molecular characterization of C. neoformans complex from environment in Beijing, China. A total of 435 samples of pigeon droppings from 11 different homes in Beijing were collected from August to November in 2015. Pigeon droppings were inoculated onto caffeic acid cornmeal agar (CACA) to screen C. neoformans complex. Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed for species identification...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28426182/usefulness-and-accuracy-of-maldi-tof-mass-spectrometry-as-a-supplementary-tool-to-identify-mosquito-vector-species-and-to-invest-in-development-of-international-database
#3
F N Raharimalala, T M Andrianinarivomanana, A Rakotondrasoa, J M Collard, S Boyer
Arthropod-borne diseases are important causes of morbidity and mortality. The identification of vector species relies mainly on morphological features and/or molecular biology tools. The first method requires specific technical skills and may result in misidentifications, and the second method is time-consuming and expensive. The aim of the present study is to assess the usefulness and accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a supplementary tool with which to identify mosquito vector species and to invest in the creation of an international database...
April 20, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28424252/comparison-of-saramis-4-12-and-ivd-3-0-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-mycobacteria-from-solid-and-liquid-culture-media
#4
Caroline Leyer, Guillaume Gregorowicz, Faiza Mougari, Laurent Raskine, Emmanuelle Cambau, Dominique de Briel
During the last decade, many publications studied the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for mycobacterial identification. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were submitted to protein extraction and same spectra were matched against both databases...
April 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#5
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28418253/species-level-discrimination-of-psychrotrophic-pathogenic-and-spoilage-gram-negative-raw-milk-isolates-using-a-combined-maldi-tof-ms-proteomics-bioinformatics-based-approach
#6
Nuwan R Vithanage, Thomas Richard Yeager, Jeevana Bhongir, Snehal R Jadhav, Chaminda Senaka Ranadheera, Enzo A Palombo, Nivedita Datta
Identification of psychrotrophic pathogenic and spoilage Gram-negative bacteria using rapid and reliable techniques is important in commercial milk processing, as these bacteria can produce heat-resistant proteases and act as post-processing contaminants in pasteurised milk. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a proven technology for identification of bacteria in food, however may require optimisation for identification of pathogenic and spoilage bacteria in milk and dairy products...
April 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28414107/antimicrobial-susceptibility-of-bacteroides-fragilis-group-organisms-in-hong-kong-by-the-tentative-eucast-disc-diffusion-method
#7
Pak-Leung Ho, Chong-Yee Yau, Lok-Yan Ho, Eileen Ling-Yi Lai, Melissa Chun-Jiao Liu, Cindy Wing-Sze Tse, Kin-Hung Chow
This study used a recently developed EUCAST disc diffusion method to measure the susceptibility of 741 B. fragilis group isolates to six antibiotics. Isolates nonsusceptible to imipenem and metronidazole by the disc method were further investigated by E-test. Species identification was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), PCR assays and 16S rRNA sequencing. The most common species were B. fragilis (n = 424, including 81 division II and 343 division I isolates), B...
April 14, 2017: Anaerobe
https://www.readbyqxmd.com/read/28409495/antiplatelet-and-anti-proliferative-action-of-disintegrin-from-echis-multisquamatis-snake-venom
#8
Volodymyr Chernyshenko, Natalia Petruk, Darya Korolova, Ludmila Kasatkina, Olha Gornytska, Tetyana Platonova, Tamara Chernyshenko, Andriy Rebriev, Olena Dzhus, Liudmyla Garmanchuk, Eduard Lugovskoy
AIM: To purify the platelet aggregation inhibitor from Echis multisquamatis snake venom (PAIEM) and characterize its effect on platelet aggregation and HeLa cell proliferation. METHODS: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) were used for PAIEM identification. Platelet aggregation in the presence of PAIEM was studied on aggregometer Solar-AP2110. The changes of shape and granularity of platelets in the presence of PAIEM were studied on flow cytometer COULTER EPICS XL, and degranulation of platelets was estimated using spectrofluorimetry...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28408060/bacterial-epidemiology-and-antimicrobial-resistance-profiles-of-urinary-specimens-of-the-elderly
#9
F Gravey, G Loggia, A de La Blanchardière, V Cattoir
OBJECTIVE: Although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. Our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. METHODS: From October 2012 to October 2015, all urinary specimens (catheter-related or not) received at the laboratory of microbiology of the university hospital of Caen (France) were retrospectively studied...
April 10, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28406696/proteomics-approach-for-identification-of-iga-nephropathy-related-biomarkers-in-urine
#10
P Prikryl, L Vojtova, D Maixnerova, M Vokurka, M Neprasova, T Zima, V Tesar
Proteinuria is often used as a surrogate marker in monitoring and predicting outcome in patients with chronic kidney diseases, but it is non-specific. IgAN belongs to the most common primary glomerulonephritis worldwide with serious prognosis. The main aim of this work was to assess differences in urine proteins in patients with IgA nephropathy and to identify abnormal proteins as potential biomarkers of IgA nephropathy or the renal disease. In our pilot project, we selected 20 patients and compared them with 20 healthy volunteers...
April 12, 2017: Physiological Research
https://www.readbyqxmd.com/read/28404947/identification-of-urine-biomarkers-associated-with-lung-adenocarcinoma
#11
Weiwei Wang, Shanshan Wang, Man Zhang
Lung adenocarcinoma (LAC) progression is accompanied by changes in protein levels that may be reflected in body fluids, such as urine. Urine collected from LAC patients (n=34) and healthy controls (n=36) was analyzed via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange magnetic beads. The results revealed 76 urinary polypeptides significantly different between LAC patients and normal controls (P<0.05). Twenty-two of these peptides were up-regulated and 54 were down-regulated...
March 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28403184/low-prevalence-of-human-enteropathogenic-yersinia-spp-in-brown-rats-rattus-norvegicus-in-flanders
#12
Lieze Oscar Rouffaer, Kristof Baert, Anne-Marie Van den Abeele, Ivo Cox, Gerty Vanantwerpen, Lieven De Zutter, Diederik Strubbe, Katleen Vranckx, Luc Lens, Freddy Haesebrouck, Michel Delmée, Frank Pasmans, An Martel
Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method...
2017: PloS One
https://www.readbyqxmd.com/read/28399425/direct-identification-from-positive-blood-broth-culture-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#13
Maria Goreth Barberino, Marcio Silva Oliveira, Ana Carolina Palmeiras Arraes, Luís Cláudio Correia, Ana Verena Mendes
Bloodstream infections (BSIs) are among the most concerning bacterial infections. They are one of the leading causes of morbidity and mortality, and occur in 30-70% of critical care patients. The prompt identification of the causative microorganism can help choosing the appropriate antimicrobial therapy that will lead to better clinical outcomes. Blood culture is one of the most relevant tests for microbiological diagnosis of bacterial infections. The introduction of the MALDI-TOF microbiological diagnosis significantly decreased the time of identifying microorganisms...
April 8, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#14
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza Hernandez, Halil Er, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397470/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-early-identification-of-septic-patients
#15
Ali O Sekercioglu, Yesim Cekin, Dilara Ogunc, Gozde Ongut, Betil O Baysan, Dilek Colak, Filiz Gunseren, Levent Donmez
BACKGROUND: Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful technique for the rapid identification of bacteria from growing colonies in routine cultures. In this study, we evaluated the feasibility of a 5-hour incubation on solid medium after sub-cultivation of positive blood culture broth without any preparation steps in order to speed up the identification of bacteria. METHODS: In addition to standard laboratory protocols, a Columbia agar plate with 5% sheep blood was inoculated with 1 drop from the blood culture broth...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397166/characterization-of-igg-glycosylation-in-rheumatoid-arthritis-patients-by-maldi-tof-ms-n-and-capillary-electrophoresis
#16
Chuncui Huang, Yaming Liu, Hongmei Wu, Dehui Sun, Yan Li
An analytical method based on the combination of multistage mass spectrometry (MS(n)) and capillary electrophoresis (CE) was developed for the analysis of immunoglobulin G (IgG) glycosylation in rheumatoid arthritis (RA) patients. It has been recently suggested that IgG glycosylation defect may be involved in RA immunopathogenesis. Complete characterization of glycans, including both qualitative and quantitative analysis, requires a combination of different techniques, and accurate, robust, sensitive, and high-throughput methodologies are important for analysis of clinical samples...
April 10, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28393464/exo-and-surface-proteomes-of-the-probiotic-bacterium-lactobacillus-acidophilus-ncfm
#17
Hasan Ufuk Celebioglu, Birte Svensson
Lactobacillus acidophilus NCFM is a well-known probiotic bacterium extensively studied for its beneficial health effects. Exoproteome (proteins exported into culture medium) and surface proteome (proteins attached to S-layer) of this probiotic were identified by using 2DE followed by MALDI TOF MS to find proteins potentially involved in bacteria-host interactions. The exo- and surface proteomes included 43 and 39 different proteins from 72 and 49 successfully identified spots, respectively. Twenty-two proteins were shared between the two proteomes; both contained the major surface layer protein that participates in host interaction as well as several well-known and putative moonlighting proteins...
April 9, 2017: Proteomics
https://www.readbyqxmd.com/read/28385661/intact-cell-maldi-tof-mass-spectrometry-on-single-bovine-oocyte-and-follicular-cells-combined-with-top-down-proteomics-a-novel-approach-to-characterise-markers-of-oocyte-maturation
#18
Valérie Labas, Ana-Paula Teixeira-Gomes, Laura Bouguereau, Audrey Gargaros, Lucie Spina, Aurélie Marestaing, Svetlana Uzbekova
Intact cell MALDI-TOF mass spectrometry (ICM-MS) was adapted to bovine follicular cells from individual ovarian follicles to obtain the protein/peptide signatures (<17kDa) of single oocytes, cumulus cells (CC) and granulosa cells (GC), which shared a total of 439 peaks. By comparing the ICM-MS profiles of single oocytes with CC before and after in vitro maturation (IVM), 71 different peaks were characterised, and their relative abundance was found to vary depending on the stage of oocyte meiotic maturation...
April 3, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28385567/the-first-case-report-of-infective-endocarditis-caused-by-gemella-taiwanensis
#19
Mayu Hikone, Naoya Sakamoto, Masayuki Ota, Takuya Washino, Ken-Ichiro Kobayashi, Sentaro Iwabuchi, Haruko Kazama, Akiko Kounosu, Kumiko Negishi, Yusuke Ainoda, Shigekazu Iguchi, Atsushi Yoshida, Ken Kikuchi, Kenji Ohnishi
Gemella is a facultative anaerobic Gram-positive coccus and a rare cause of infective endocarditis (IE). Gram staining may eventually misidentify the organism, which tends to easily decolorize and manifest as either Gram-negative or Gram-variable. Commercial biochemical tests are often used to identify Gemella, but the methods they employ sometimes lack accuracy. A 52-year-old woman was diagnosed with Gemella taiwanensis IE after initial identification of the pathogen as Gemella haemolysans using biochemical tests combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
April 3, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28384252/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-identification-of-burkholderia-pseudomallei-from-asia-and-australia-and-differentiation-between-burkholderia-species
#20
Vichaya Suttisunhakul, Apinya Pumpuang, Peeraya Ekchariyawat, Vanaporn Wuthiekanun, Mindy G Elrod, Paul Turner, Bart J Currie, Rattanaphone Phetsouvanh, David A B Dance, Direk Limmathurotsakul, Sharon J Peacock, Narisara Chantratita
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates...
2017: PloS One
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