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Maldi-tof identification

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https://www.readbyqxmd.com/read/28318230/label-free-discovery-array-platform-for-the-characterization-of-glycan-binding-proteins-and-glycoproteins
#1
Christopher John Gray, Antonio Sánchez-Ruiz, Ivana Šardzíková, Yassir A Ahmed, Rebecca Louise Miller, Juana E Reyes Martinez, Edward Pallister, Kun Huang, Peter Both, Mirja Hartmann, Hannah N Roberts, Robert Šardzik, Santanu Mandal, Jeremy E Turnbull, Claire E Eyers, Sabine L Flitsch
The identification of carbohydrate-protein interactions is central to our understanding of the roles of cell-surface carbohydrates (the glycocalyx), fundamental for cell-recognition events. Therefore there is a need for fast high-throughput biochemical tools to capture the complexity of these biological interactions. Here we describe a rapid method for qualitative label-free detection of carbohydrate-protein interactions on arrays of simple synthetic glycans, more com-plex natural glycosaminoglycans (GAG) and lectins/carbohydrate binding proteins using MALDI-ToF mass spectrometry...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28306235/gcib-tof-sims-high-resolution-imaging-of-cardiolipin-speciation-in-the-brain-identification-of-molecular-losses-after-traumatic-injury
#2
Hua Tian, Louis J Sparvero, Andrew A Amoscato, Anna Bloom, Hulya Bayir, Valerian E Kagan, Nicholas Winograd
Gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) has shown the full potential of mapping intact lipids in biological systems with better than 10 µm lateral resolution. This study investigated further the capability of GCIB-SIMS in imaging high-mass signals from intact cardiolipin (CL) and gangliosides in normal brain and the effect of a controlled cortical impact model (CCI) of traumatic brain injury (TBI) on their distribution. A combination of enzymatic and chemical treatments was employed to suppress the signals from the most abundant phospholipids (phosphatidylcholine (PC) and phosphatidylethanolamine (PE)) and enhance the signals from the low abundance CLs and gangliosides to allow their GCIB-SIMS detection at 8 and 16 µm spatial resolution...
March 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28303824/matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-rapid-detection-of-carbapenamase-activity-in-acinetobacter-baumannii-isolates
#3
Noha Abouseada, May Raouf, Eman El-Attar, Pacinte Moez
INTRODUCTION: Carbapenamase-producing Acinetobacter baumannii are an increasing threat in hospitals and Intensive Care Units. Accurate and rapid detection of carbapenamase producers has a great impact on patient improvement and aids in implementation of infection control measures. AIM: In this study, we describe the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI TOF MS) to identify carbapenamase-producing A. baumannii isolates in up to 3 h...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303121/identification-of-lama-glama-as-reservoirs-for-acinetobacter-lwoffii
#4
Martín M Ledesma, Ailén M Díaz, Claudia Barberis, Carlos Vay, Marcela A Manghi, Juliana Leoni, Marisa S Castro, Alejandro Ferrari
South American Camelids have an increasing relevance in local economies, worldwide. These animals are bred for their meat, fur and as companion and therapy animals. Thus, their sanitary status should be well-established. According to the OIE (World Organization for Animal Health), respiratory infections mainly produced by Pasteurella spp. have been reported for camelids. It has been stated that this microorganism causes a mild disease, although many authors report it is an important cause of mortality among alpacas...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28290329/yeasts-from-canastra-cheese-production-process-isolation-and-evaluation-of-their-potential-for-cheese-whey-fermentation
#5
Rafaela Pereira Andrade, Carolina Naves Melo, Zlatina Genisheva, Rosane Freitas Schwan, Whasley Ferreira Duarte
Canastra cheese is a cheese with geographical indication recognized by the Brazilian National Institute of Industrial Protection under number IG201002. It is produced in seven municipalities in the state of Minas Gerais in a region called Serra da Canastra. In this work, samples of milk, "pingo" (natural starter), whey and Canastra cheese were collected on a farm in Medeiros-MG/Brazil to evaluate the yeast microbiota and select yeasts for whey fermentation to produce ethanol and volatile aromatic compounds of relevance in the production of cheese...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28285496/maldi-tof-mass-spectrometry-as-a-useful-tool-for-identification-of-enterococcus-spp-from-wild-birds-and-differentiation-of-closely-related-species
#6
Dagmara Stępień-Pyśniak, Tomasz Hauschild, Paweł Różański, Agnieszka Marek
The aim of this study was to explore the accuracy and feasibility of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in identifying bacteria from environmental sources, as compared to rpoA gene sequencing, and to evaluate the occurrence of bacteria of the genus Enterococcus in wild birds. In addition, a phyloproteomic analysis of certain Enterococcus species with spectral relationships was performed. The enterococci were isolated from 25 species of wild birds in central Europe (Poland)...
March 13, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28284599/the-oral-microbiota-of-domestic-cats-harbors-a-wide-variety-of-staphylococcus-species-with-zoonotic-potential
#7
Ciro César Rossi, Ingrid da Silva Dias, Igor Mansur Muniz, Walter Lilenbaum, Marcia Giambiagi-deMarval
This study aimed to characterize the species, antimicrobial resistance and dispersion of CRISPR systems in staphylococci isolated from the oropharynx of domestic cats in Brazil. Staphylococcus strains (n=75) were identified by MALDI-TOF and sequencing of rpoB and tuf genes. Antimicrobial susceptibility was assessed by disk diffusion method and PCR to investigate the presence of antimicrobial-resistance genes usually present in mobile genetic elements (plasmids), in addition to plasmid extraction. CRISPR - genetic arrangements that give the bacteria the ability to resist the entry of exogenous DNA - were investigated by the presence of the essential protein Cas1 gene...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28283012/-evaluation-of-mascia-brunelli-rapid-antigen-test-in-the-diagnosis-of-group-a-streptococcal-pharyngitis
#8
Ayşe Barış, Nur Anlıaçık, Mehmet Emin Bulut, Rıdvan Deniz, Elif Yücel, Elif Aktaş
Pharyngitis in most cases is due to viral microorganisms however drug therapy without the detection of etiological agent leads to unnecessary use of antibiotics. On the other hand, when the etiologic agent is group A beta-hemolytic streptococci (GAS) it is important to identify the etiologic agent rapidly which will guide the treatment with appropriate antibiotics. The use of highly sensitive rapid tests will contribute significantly to early diagnosis and appropriate therapy. The aim of this study is to evaluate the efficacy of Mascia Brunelli rapid antigen test for the detection of GAS in throat swab samples...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#9
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28274205/rapid-detection-of-carbapenemase-producing-klebsiella-pneumoniae-strains-derived-from-blood-cultures-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#10
Christina Sakarikou, Marco Ciotti, Camilla Dolfa, Silvia Angeletti, Cartesio Favalli
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE), particularly carbapenemase-producing Klebsiella pneumoniae isolates, are important causative agents of nosocomial infections associated with significant mortality rates mostly in critical wards. The rapid detection and typing of these strains is critical either for surveillance purposes and to prevent outbreaks and optimize antibiotic therapy. In this study, the MALDI-TOF MS method was used to detect rapidly these isolates from blood cultures (BCs) and to obtain proteomic profiles enable to discriminate between carbapenemase-producing and non-carbapenemase-producing strains...
March 8, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28273807/simplifying-the-preparation-of-pollen-grains-for-maldi-tof-ms-classification
#11
Franziska Lauer, Stephan Seifert, Janina Kneipp, Steffen M Weidner
Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a well-implemented analytical technique for the investigation of complex biological samples. In MS, the sample preparation strategy is decisive for the success of the measurements. Here, sample preparation processes and target materials for the investigation of different pollen grains are compared. A reduced and optimized sample preparation process prior to MALDI-TOF measurement is presented using conductive carbon tape as target...
March 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28273492/enhanced-ovalbumin-stability-at-oil-water-interface-by-phosphorylation-and-identification-of-phosphorylation-site-using-maldi-tof-mass-spectrometry
#12
Zhouyi Xiong, Meihu Ma
To improve the interfacial properties, a phosphorylation modification of OVA was performed through dry-heating at three different pH values (5.0, 7.0 and 9.0) in the presence of sodium tripolyphosphate. X-ray photoelectron and Raman spectroscopies confirmed that phosphate groups were successfully grafted onto the ovalbumin backbone through covalent interaction to form OP bond. Additionally, 23, 21 and 18 phosphorylation sites were identified in the OVA that had been phosphorylated at pH 5.0, 7.0 and 9.0 (P-OVA5, P-OVA7 and P-OVA9) respectively by MALDI-TOF mass spectroscopy...
February 21, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28273152/pre-and-post-weaning-scours-in-southeastern-australia-a-survey-of-22-commercial-pig-herds-and-characterisation-of-escherichia-coli-isolates
#13
Lechelle K Van Breda, Om P Dhungyel, Andrew N Ginn, Jonathan R Iredell, Michael P Ward
Diarrhoeal diseases in piglets caused by Escherichia coli are responsible for substantial losses each year in the Australian pig industry. A cross-sectional survey was conducted (September 2013-May 2014) across 22 commercial pig herds located in southeastern Australia: NSW (n = 9); VIC (n = 10); and SA (n = 3), to estimate the prevalence of E. coli associated diarrhoea in pre- and post-weaned piglets and to identify key risk factors associated with E. coli disease. A questionnaire on management and husbandry practices was included...
2017: PloS One
https://www.readbyqxmd.com/read/28267570/identification-of-microorganisms-grown-on-chromogenic-media-by-maldi-tof-ms
#14
Petra Lüthje, Arthur B Pranada, Duncan Carruthers-Lay, Marc Desjardins, Olivier Gaillot, David Wareham, Holly Ciesielczuk, Volkan Özenci
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS...
March 4, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28264610/corynebacterium-species-causing-breast-abscesses-among-patients-attending-a-tertiary-care-hospital-in-chennai-south-india
#15
Indira Poojary, Ann Kurian, Jayalekshmi V A, Debora Devapriya J, Thirunarayan M A
BACKGROUND: Corynebacterium species other than Corynebacterium diphtheriae were mostly considered contaminants in the past, but there are reports of their association with wide variety of human infections lately. In this study, we look into Corynebacterium species isolated from breast abscess patients and assess their antimicrobial susceptibility pattern and treatment outcomes. MATERIAL AND METHODS: Pus samples from suspected breast abscess cases were examined from October 2014 to September 2015...
March 6, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28262370/species-identification-of-streptococcus-bovis-group-isolates-causing-bacteremia-a-comparison-of-two-maldi-tof-ms-systems
#16
Charlotte N Agergaard, Elisa Knudsen, Rimtas Dargis, Xiaohui C Nielsen, Jens J Christensen, Ulrik S Justesen
This study compared two MALDI-TOF MS systems (Biotyper and VITEK MS) on clinical Streptococcus bovis group isolates (n=66). The VITEK MS gave fewer misidentifications and a higher rate of correct identifications than the Biotyper. Only the identification of S. lutetiensis by the VITEK MS was reliable. Additional optimization of the available system databases is needed.
February 20, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28261610/cervical-cancer-cell-line-secretome-highlights-the-roles-of-transforming-growth-factor-beta-induced-protein-ig-h3-peroxiredoxin-2-and-nrf2-on-cervical-carcinogenesis
#17
Georgia Kontostathi, Jerome Zoidakis, Manousos Makridakis, Vasiliki Lygirou, George Mermelekas, Theofilos Papadopoulos, Konstantinos Vougas, Alexios Vlamis-Gardikas, Peter Drakakis, Dimitrios Loutradis, Antonia Vlahou, Nicholas P Anagnou, Kalliopi I Pappa
Cancer cells acquire unique secretome compositions that contribute to tumor development and metastasis. The aim of our study was to elucidate the biological processes involved in cervical cancer, by performing a proteomic analysis of the secretome from the following informative cervical cell lines: SiHa (HPV16+), HeLa (HPV18+), C33A (HPV-), and HCK1T (normal). Proteins were analyzed by 2D gel electrophoresis coupled to MALDI-TOF-MS. Enrichment of secreted proteins with characteristic profiles for each cell line was followed by the identification of differentially expressed proteins...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28257640/risk-factors-for-candida-parapsilosis-bloodstream-infection-in-a-neonatal-intensive-care-unit-a-case-control-study
#18
Carmine Garzillo, Maria Bagattini, Lidija Bogdanović, Anna Di Popolo, Vita Dora Iula, Maria Rosaria Catania, Francesco Raimondi, Maria Triassi, Raffaele Zarrilli
BACKGROUND: Candida parapsilosis is increasingly responsible for invasive candidiasis in neonates. This study investigates phenotypic and genotypic features of C. parapsilosis microbial isolates and underlying clinical conditions associated with acquisition of C. parapsilosis in a neonatal intensive care unit (NICU) in Italy. METHODS: Identification of C. parapsilosis was performed by VITEK® 2 and MALDI TOF and confirmed by analysis of internal transcribed spacer ribosomal DNA sequences...
January 19, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28257074/microcystin-lr%C3%A2-detected%C3%A2-in%C3%A2-a%C3%A2-low%C3%A2-molecular%C3%A2-weight%C3%A2-fraction%C3%A2-from%C3%A2-a%C3%A2-crude%C3%A2-extract%C3%A2-of%C3%A2-zoanthus%C3%A2-sociatus
#19
Dany Domínguez-Pérez, Armando Alexei Rodríguez, Hugo Osorio, Joana Azevedo, Olga Castañeda, Vítor Vasconcelos, Agostinho Antunes
Cnidarian constitutes a great source of bioactive compounds. However, research involving peptides from organisms belonging to the order Zoanthidea has received very little attention, contrasting to the numerous studies of the order Actiniaria, from which hundreds of toxic peptides and proteins have been reported. In this work, we performed a mass spectrometry analysis of a low molecular weight (LMW) fraction previously reported as lethal to mice. The low molecular weight (LMW) fraction was obtained by gel filtration of a Zoanthus sociatus (order Zoanthidea) crude extract with a Sephadex G-50, and then analyzed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry (MS) in positive ion reflector mode from m/z 700 to m/z 4000...
March 1, 2017: Toxins
https://www.readbyqxmd.com/read/28254444/comparison-of-growth-on-mannitol-salt-agar-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-vitek-%C3%A2-2-with-partial-sequencing-of-16s-rrna-gene-for-identification-of-coagulase-negative-staphylococci
#20
Funmilola A Ayeni, Camilla Andersen, Niels Nørskov-Lauritsen
Mannitol salt agar (MSA) is often used in resources' limited laboratories for identification of S. aureus however, coagulase-negative staphylococci (CoNS) grows and ferments mannitol on MSA. 171 strains of CoNS which have been previously misidentified as S. aureus due to growth on MSA were collected from different locations in Nigeria and two methods for identification of CoNS were compared i.e. ViTEK 2 and MALDI-TOF MS with partial 16S rRNA gene sequencing as gold standard. Partial tuf gene sequencing was used for contradicting identification...
February 28, 2017: Microbial Pathogenesis
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