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Maldi-tof identification

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https://www.readbyqxmd.com/read/28811116/identification-of-nocardia-streptomyces-and-tsukamurella-using-maldi-tof-ms-with-the-bruker-biotyper
#1
Melanie L Yarbrough, William Lainhart, Carey-Ann D Burnham
Nocardia species are the most commonly isolated aerobic actinomycetes from human clinical specimens. Our objective was to assess the identification of clinically relevant actinomycetes using the Bruker Biotyper MALDI-TOF system, including comparison of extraction methods, Biotyper library versions, score cutoffs, and media. Banked Streptomyces (n=10), Tsukamurella (n=2), and Nocardia isolates (n=60) were cultured and extracted using three methods: mycobacterial extraction, ethanol formic acid extraction, or direct on-target extraction...
July 1, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28802703/identification-and-characterization-of-clostridium-botulinum-group-iii-field-strains-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#2
Luca Bano, Ilenia Drigo, Elena Tonon, Simone Pascoletti, Cinzia Puiatti, Fabrizio Anniballi, Bruna Auricchio, Florigio Lista, Cesare Montecucco, Fabrizio Agnoletti
Animal botulism is primarily due to botulinum neurotoxin (BoNT) types C, D or their chimeric variants C/D or D/C, produced by Clostridium botulinum group III, which appears to include the genetically indistinguishable Clostridium haemolyticum and Clostridium novyi. In the present study, we used matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI TOF MS) to identify and characterize 81 BoNT-producing Clostridia isolated in 47 episodes of animal botulism. The instrument's default database, containing no entries for Clostridium botulinum, permitted reliable identification of 26 strains at the genus level...
August 9, 2017: Anaerobe
https://www.readbyqxmd.com/read/28802585/genomics-of-the-new-species-kingella-negevensis-diagnostic-issues-identification-of-a-locus-encoding-a-rtx-toxin
#3
Onya Opota, Sacha Laurent, Trestan Pillonel, Marie Léger, Sabrina Trachsel, Guy Prod'hom, Katia Jaton, Gilbert Greub
Kingella kingae, producing the cytotoxic RTX protein, is a causative agent of serious infections in humans such as bacteremia, endocarditis and osteoarticular infection, especially in young children. Recently, Kingella negevensis, a related species, has been isolated from the oral cavity of healthy children. In this study, we report the isolation of K. negevensis strain eburonensis, initially misidentified as K. kingae with MALDI-TOF MS, from a vaginal specimen of a patient suffering of vaginosis. The genome sequencing and analysis of this strain together with comparative genomics of the Kingella genus revealed that K...
August 9, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/28801691/a-new-data-processing-routine-facilitating-the-identification-of-surface-adhered-proteins-from-bacterial-conditioning-films-via-qcm-d-maldi-tof-ms
#4
Siegfried Hohmann, Anke Neidig, Boris Kühl, Frank Kirschhöfer, Jörg Overhage, Gerald Brenner-Weiß
Conditioning films are an important factor in the initiation and development of microbial biofilms, which are the leading cause of chronic infections associated with medical devices. Here, we analyzed the protein content of conditioning films formed after exposure to supernatants of cultures of the human pathogen Pseudomonas aeruginosa PAO1. Adhesion of substances from the supernatant was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) sensor chips modified with the commonly used implant material titanium dioxide (TiO2)...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801089/clinical-and-microbiological-characteristics-of-patients-with-bacteremia-caused-by-campylobacter-species-with-an-emphasis-on-the-subspecies-of-c-fetus
#5
Yen-Hung Liu, Wataru Yamazaki, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, Po-Ren Hsueh
OBJECTIVES: This study was intended to investigate the clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species. METHODS: From April 1998 to May 2014, 56 adults with bacteremia caused by Campylobacter species were evaluated. These Campylobacter species isolates were confirmed to the species level using 16S rRNA gene sequencing (all isolates) and multiplex PCR analysis (for C. fetus only). The performance of identification for Campylobacter species by the Bruker Biotyper MALDI-TOF MS was evaluated...
July 25, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28797803/a-multi-center-ring-trial-for-the-identification-of-anaerobic-bacteria-using-maldi-tof-ms
#6
A C M Veloo, H Jean-Pierre, U S Justesen, T Morris, E Urban, I Wybo, H N Shah, A W Friedrich, T Morris, H N Shah, H Jean-Pierre, U S Justesen, E Nagy, E Urban, M Kostrzewa, A Veloo, A W Friedrich
Inter-laboratory reproducibility of Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of anaerobic bacteria has not been shown before. Therefore, ten anonymized anaerobic strains were sent to seven participating laboratories, an initiative of the European Network for the Rapid Identification of Anaerobes (ENRIA). On arrival the strains were cultured and identified using MALDI-TOF MS. The spectra derived were compared with two different Biotyper MALDI-TOF MS databases, the db5627 and the db6903...
August 7, 2017: Anaerobe
https://www.readbyqxmd.com/read/28796186/maldi-tof-ms-for-the-identification-of-cultivable-organic-degrading-bacteria-in-contaminated-groundwater-near-unconventional-natural-gas-extraction-sites
#7
Inês C Santos, Misty S Martin, Doug D Carlton, Catarina L Amorim, Paula M L Castro, Zacariah L Hildenbrand, Kevin A Schug
Groundwater quality and quantity is of extreme importance as it is a source of drinking water in the United States. One major concern has emerged due to the possible contamination of groundwater from unconventional oil and natural gas extraction activities. Recent studies have been performed to understand if these activities are causing groundwater contamination, particularly with respect to exogenous hydrocarbons and volatile organic compounds. The impact of contaminants on microbial ecology is an area to be explored as alternatives for water treatment are necessary...
August 10, 2017: Microorganisms
https://www.readbyqxmd.com/read/28792758/profiling-of-microbial-colonies-for-high-throughput-engineering-of-multi-step-enzymatic-reactions-via-optically-guided-maldi-ms
#8
Tong Si, Bin Li, Troy J Comi, Yuwei Wu, Pingfan Hu, Yuying Wu, Yuhao Min, Douglas A Mitchell, Huimin Zhao, Jonathan V Sweedler
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry (MS) imaging has been used for rapid phenotyping of enzymatic activities, but is mainly limited to single-step conversions. Herein we report a label-free method for high-throughput engineering of multi-step biochemical reactions based on optically guided MALDI-ToF MS analysis of bacterial colonies. The bacterial cells provide containment of multiple enzymes and access to substrates and cofactors via metabolism. Automated MALDI-ToF MS acquisition from randomly distributed colonies simplifies procedures to prepare strain libraries without liquid handling...
August 9, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28782649/comparison-of-methods-available-for-the-identification-of-mycobacterium-chimaera
#9
Emmanuel Lecorche, Sophie Haenn, Faiza Mougari, Sylvain Kumanski, Nicolas Veziris, Hanaa Benmansour, Laurent Raskine, Laurent Moulin, Emmanuelle Cambau
OBJECTIVES: Mycobacterium chimaera is a recently described nontuberculous mycobacterium belonging to the M. avium complex (MAC). Because this species is implicated in a worldwide outbreak due to contaminated heater-cooler unit water tanks during open-heart surgery, it becomes mandatory for clinical microbiology laboratories to be able to differentiate M. chimaera from the other MAC species, especially M. intracellulare. Such identification was so far restricted to specialized laboratories, since it required the analysis of several gene sequences...
August 4, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28780272/current-challenges-in-the-accurate-identification-of-streptococcus-pneumoniae-and-its-serogroups-serotypes-in-the-vaccine-era
#10
REVIEW
Rosemol Varghese, Ranjith Jayaraman, Balaji Veeraraghavan
Streptococcus pneumoniae is a major cause of pneumonia, meningitis and other invasive diseases resulting in high mortality and morbidity among children under the age of five. Inaccurate identification of S. pneumoniae masks the exact estimation of disease burden and could delay treatment options. This is the common problem most frequently faced in developing countries due to several reasons that include poor infrastructure, insensitive operational procedures and lack of expertise. Inconsistent methods for phenotypic detection often delay the early identification and confirmation of S...
August 2, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28776045/whole-genome-sequencing-of-human-clinical-klebsiella-pneumoniae-isolates-reveals-misidentification-and-misunderstandings-of-klebsiella-pneumoniae-klebsiella-variicola-and-klebsiella-quasipneumoniae
#11
S Wesley Long, Sarah E Linson, Matthew Ojeda Saavedra, Concepcion Cantu, James J Davis, Thomas Brettin, Randall J Olsen
Klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. The emergence of highly drug-resistant strains is particularly concerning. There has been a recognition and division of Klebsiella pneumoniae into three distinct phylogenetic groups: Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae. K. variicola and K. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans than K. pneumoniae does...
July 2017: MSphere
https://www.readbyqxmd.com/read/28769078/assessment-of-a-novel-bile-solubility-test-and-maldi-tof-for-the-differentiation-of-streptococcus-pneumoniae-from-other-mitis-group-streptococci
#12
Hans-Christian Slotved, Richard R Facklam, Kurt Fuursted
This study assesses a novel bile solubility test and MALDI-TOF for the differentiation of Streptococcus pneumoniae from other mitis group streptococci, including differentiation of S. pneumoniae from Streptococcus pseudopneumoniae. Eighty-four species verified mitis group isolates were subjected to our bile solubility test (which measures and calculates the differences of absorbance in the test tube containing 10% sodium deoxycholate versus a blank control tube, after incubation for 10 minutes at 36 °C using a spectrophotometer) and MALDI-TOF MS (both the standard result output and by visual spectra evaluation)...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28768561/field-application-of-maldi-tof-ms-on-mosquito-larvae-identification
#13
Amira Nebbak, Sekou Koumare, Alexandra C Willcox, Jean-Michel Berenger, Didier Raoult, Lionel Almeras, Philippe Parola
In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an efficient tool for arthropod identification. Its application for field monitoring of adult mosquitoes was demonstrated, but identification of larvae has been limited to laboratory-reared specimens. Study aim was to test the success of MALDI-TOF MS in correctly identifying mosquito larvae collected in the field. Collections were performed at 13 breeding sites in urban areas of Marseille, a city in the South of France...
August 3, 2017: Parasitology
https://www.readbyqxmd.com/read/28768559/maldi-tof-ms-protein-profiling-for-the-rapid-identification-of-chagas-disease-triatomine-vectors-and-application-to-the-triatomine-fauna-of-french-guiana
#14
Maureen Laroche, Jean-Michel Bérenger, Gladys Gazelle, Denis Blanchet, Didier Raoult, Philippe Parola
Triatomines are haematophagous insects involved in the transmission of Trypanosoma cruzi, the aetiological agent of Chagas disease. The vector competence of these arthropods can be highly variable, depending on the species. A precise identification is therefore crucial for the epidemiological surveillance of T. cruzi and the determination of at-risk human populations. To circumvent the difficulties of morphological identification and the lack of comprehensiveness of the GenBank database, we hereby propose an alternative method for triatomine identification...
August 3, 2017: Parasitology
https://www.readbyqxmd.com/read/28765873/classification-of-cancer-cell-lines-using-matrix-assisted-laser-desorption-ionization-time%C3%A2-of%C3%A2-flight-mass-spectrometry-and-statistical-analysis
#15
Vlad Serafim, Ajit Shah, Maria Puiu, Nicoleta Andreescu, Dorina Coricovac, Alexander Nosyrev, Demetrios A Spandidos, Aristides M Tsatsakis, Cristina Dehelean, Iulia Pinzaru
Over the past decade, matrix-assisted laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) has been established as a valuable platform for microbial identification, and it is also frequently applied in biology and clinical studies to identify new markers expressed in pathological conditions. The aim of the present study was to assess the potential of using this approach for the classification of cancer cell lines as a quantifiable method for the proteomic profiling of cellular organelles...
July 27, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28764161/providencia-rettgeri-an-emerging-nosocomial-uropathogen-in-an-indwelling-urinary-catheterised-patient
#16
Siddharth Sagar, Nagalakshmi Narasimhaswamy, Jessica D'Souza
Providencia rettgeri (P. rettgeri) is an ubiquitous organism but is seldom associated with human disease. We report the isolation of P. rettgeri from the urine sample of a 39-year-old male patient on prolonged Foley's catheterisation following a severe head injury. Identification of this organism was done by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF) based systems. P. rettgeri is an emerging pathogen among long term catheterised patients. It reflects its ability to form biofilm on the surface of the indwelling catheter as well as the inherent urease producing property of the pathogen in question as a possible mechanism of pathogenesis...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28763943/sugarcane-cells-as-origin-of-acid-beverage-floc-in-cane-sugar
#17
Roberta Bergamin Lima, Magda Andreia Tessmer, Beatriz Appezzato da Gloria, Fabio Neves Dos Santos, Marcos Nogueira Eberlin, Claudio Lima de Aguiar
Brazil stands out as the largest producer of crystal sugar in the world, exporting much of its production to the soft drinks industry. However, the chemical composition of sugar may contain numerous compounds that promote the formation of acid beverage flocs (ABF), reducing product acceptance. This study aimed to identify the chemical composition of ABF using different analytical techniques. We could observe the ABF are formed by several chemical classes. Regarding the histochemical analysis, we observed the presence of cellular sugarcane tissues, which are not fully removed in sugarcane processing...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28761915/data-for-proteome-analysis-of-bacillus-lehensis-g1-in-starch-containing-medium
#18
How Lie Ling, Zaidah Rahmat, Abdul Munir Abdul Murad, Nor Muhammad Mahadi, Rosli Md Illias
Bacillus lehensis G1 is a cyclodextrin glucanotransferase (CGTase) producer, which can degrade starch into cyclodextrin. Here, we present the proteomics data of B. lehensis cultured in starch-containing medium, which is related to the article "Proteome-based identification of signal peptides for improved secretion of recombinant cyclomaltodextrin glucanotransferase in Escherichia coli" (Ling et. al, in press). This dataset was generated to better understand the secretion of proteins involved in starch utilization for bacterial sustained growth...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28757016/characterization-of-corynebacterium-diphtheriae-c-confusum-and-c-amycolatum-isolated-from-sows-with-genitourinary-infection
#19
André P Poor, Luisa Z Moreno, Carlos E C Matajira, Beatriz M Parra, Vasco T M Gomes, Ana Paula S Silva, Mauricio C Dutra, Ana Paula G Christ, Mikaela R F Barbosa, Maria Inês Z Sato, Andrea M Moreno
Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28754475/detection-of-enterotoxin-and-protease-genes-among-hungarian-clinical-bacteroides-fragilis-isolates
#20
Károly Péter Sárvári, József Sóki, Miklós Iván, Cecília Miszti, Krisztina Latkóczy, Szilvia Zsóka Melegh, Edit Urbán
Bacteroides fragilis as a commensal bacterium is a member of the human intestinal flora, but as an opportunistic pathogen it can cause serious infections as well. Some of them, harbouring an enterotoxin gene (bft), may cause diarrhoea mainly in young children. Recently it has been shown that a member of C11 proteases called fragipain (fpn) can activate the enterotoxin, while C10 protease (bfp) is suspected of playing an important role in the invasiveness of the B. fragilis isolates. The objective of this study was to investigate the prevalence and distribution of the bft isotypes in 200 Hungarian B...
July 25, 2017: Anaerobe
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