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Maldi-tof identification

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https://www.readbyqxmd.com/read/29778469/communications-of-staphylococcus-aureus-and-non-aureus-staphylococcus-species-from-bovine-intramammary-infections-and-teat-apex-colonization
#1
Yasser S Mahmmod, Ilka Christine Klaas, Line Svennesen, Karl Pedersen, Hanne Ingmer
The role of non-aureus staphylococci (NAS) in the risk of acquisition of intramammary infections with Staphylococcus aureus is vague and still under debate. The objectives of this study were to (1) investigate the distribution patterns of NAS species from milk and teat skin in dairy herds with automatic milking systems, and (2) examine if the isolated NAS influences the expression of S. aureus virulence factors controlled by the accessory gene regulator (agr) quorum sensing system. In 8 herds, 14 to 20 cows with elevated somatic cell count were randomly selected for teat skin swabbing and aseptic quarter foremilk samples from right hind and left front quarters...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29777688/proteomic-changes-during-adult-stage-in-pre-optic-hypothalamus-hippocampus-and-pituitary-regions-of-female-rat-brain-following-neonatal-exposure-to-estradiol-17%C3%AE
#2
Vijayakumar Govindaraj, Radhika Nagamangalam Shridharan, Addicam Jagannadha Rao
Although neonatal exposure to estrogen or estrogenic compounds results in irreversible changes in the brain function and reproductive abnormalities during adulthood but the underlying mechanisms are still largely unknown. The present study has attempted to compare the protein profiles of sexually dimorphic brain regions of adult female rats which were exposed to estradiol- 17β during neonatal period. The total proteins extracted from pre-optic area (POA), hypothalamus, hippocampus and pituitary of control and neonatally E2 treated female rats was subjected to 2D-SDS-PAGE and differentially expressed proteins were identification by MALDI TOF/TOF-MS...
May 16, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29777151/integrated-proteomics-genomics-metabolomics-approaches-reveal-oxalic-acid-as-pathogenicity-factor-in-tilletia-indica-inciting-karnal-bunt-disease-of-wheat
#3
Vishakha Pandey, Manoj Singh, Dinesh Pandey, Anil Kumar
Tilletia indica incites Karnal bunt (KB) disease in wheat. To date, no KB resistant wheat cultivar could be developed due to non-availability of potential biomarkers related to pathogenicity/virulence for screening of resistant wheat genotypes. The present study was carried out to compare the proteomes of T. indica highly (TiK) and low (TiP) virulent isolates. Twenty one protein spots consistently observed as up-regulated/differential in the TiK proteome were selected for identification by MALDI-TOF/TOF. Identified sequences showed homology with fungal proteins playing essential role in plant infection and pathogen survival, including stress response, adhesion, fungal penetration, invasion, colonization, degradation of host cell wall, signal transduction pathway...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29776363/rapid-detection-of-carbapenemase-activity-of-enterobacteriaceae-isolated-from-positive-blood-cultures-by-maldi-tof-ms
#4
Jiajia Yu, Jingxian Liu, Yuanrui Li, Jing Yu, Weinan Zhu, Ying Liu, Lisong Shen
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians...
May 18, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29774958/effect-of-trapping-method-on-species-identification-of-phlebotomine-sandflies-by-maldi-tof-ms-protein-profiling
#5
P Halada, K Hlavackova, J Risueño, E Berriatua, P Volf, V Dvorak
Sandflies (Diptera: Psychodidae) (Newstead, 1911) are blood-feeding insects that transmit human pathogens including Leishmania (Trypanosomatida: Trypanosomatidae) parasites, causative agents of the leishmaniases. To elucidate Leishmania transmission cycles, conclusive identification of vector species is essential. Molecular approaches including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein profiling have recently emerged to complement morphological identification...
May 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29770892/identification-of-n-terminally-truncated-derivatives-of-insulin-analogs-formed-in-pharmaceutical-formulations
#6
Joanna Zielińska, Jacek Stadnik, Anna Bierczyńska-Krzysik, Dorota Stadnik
PURPOSE: Isolation and identification of unknown impurities of recombinant insulin lispro (produced at IBA) formed during accelerated stability testing of pharmaceutical solutions. For comparative purposes also commercially available formulations of recombinant human insulin (Humulin S®; Lilly), recombinant insulin lispro (Humalog®; Lilly), recombinant insulin aspart (NovoRapid® Penfill®; Novo Nordisk), recombinant insulin detemir (Levemir®; Novo Nordisk) and recombinant insulin glargine (Lantus®; Sanofi-Aventis) were analyzed...
May 16, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29765361/biodiversity-of-environmental-leptospira-improving-identification-and-revisiting-the-diagnosis
#7
Roman Thibeaux, Dominique Girault, Emilie Bierque, Marie-Estelle Soupé-Gilbert, Anna Rettinger, Anthony Douyère, Michael Meyer, Gregorio Iraola, Mathieu Picardeau, Cyrille Goarant
Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira . However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29764754/detection-of-methicillin-resistant-coagulase-negative-staphylococci-harboring-the-class-a-mec-complex-by-maldi-tof-mass-spectrometry
#8
Dominik Schuster, Michaele Josten, Kathrin Janssen, Isabel Bodenstein, Cathrin Albert, Anna Schallenberg, Mike Gajdiss, Esther Sib, Christiane Szekat, Katja Kehl, Marijo Parčina, Gunnar T R Hischebeth, Gabriele Bierbaum
The aim of this study was to test the identification of methicillin resistance in coagulase-negative staphylococci by routine matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). SCCmec cassettes of type II, III and VIII encode a small peptide called PSM-mec in the vicinity of mecA. It is visible at m/z 2415 during MALDI-TOF MS of whole cells of Staphylococcus aureus. In view of the fact that psm-mec has been identified in methicillin-resistant coagulase-negative staphylococci, we evaluated a collection of clinical coagulase-negative staphylococci, that contained 77...
May 8, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29762871/fast-identification-of-dermatophytes-by-maldi-tof-ms-using-direct-transfer-of-fungal-cells-on-ground-steel-target-plates
#9
Keith C da Cunha, Arnaud Riat, Anne-Cecile Normand, Philipp P Bosshard, Margarete T G de Almeida, Renaud Piarroux, Jacques Schrenzel, Lionel Fontao
BACKGROUND: Dermatophytes cause human infections limited to keratinized tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. OBJECTIVES: We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. METHODS: We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/ testing a panel of mass spectrum produced with strains genotypically identified and, 2/ comparing MALDI-TOF/MS identification to morphology-based methods...
May 15, 2018: Mycoses
https://www.readbyqxmd.com/read/29762699/cultivable-bacteria-from-pectinatella-magnifica-and-the-surrounding-water-in-south-bohemia-indicate-potential-new-gammaproteobacterial-betaproteobacterial-and-firmicutes-taxa
#10
Hana Salmonová, Jirí Killer, Vera Bunešová, Martina Geigerová, Eva Vlková
Pectinatella magnifica is a freshwater bryozoan, which has become a subject of scientific interest because of its invasive expansion worldwide. To obtain a comprehensive overview of its influence on environments, information on associated bacteria is needed. In this study, cultivable bacteria associated with P. magnifica were investigated. In total, 253 isolates were selected for preliminary identification by MALDI-TOF mass spectrometry and clustered based on REP-PCR profiles. Among these, 169 strains were selected and identified using 16S rRNA gene comparative analyses...
May 14, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29762695/development-and-validation-of-an-extended-database-for-yeast-identification-by-maldi-tof-ms-in-argentina
#11
Constanza Giselle Taverna, Mariana Mazza, Nadia Soledad Bueno, Christian Alvarez, Susana Amigot, Mariana Andreani, Natalia Azula, Rubén Barrios, Norma Fernández, Barbara Fox, Liliana Guelfand, Ivana Maldonado, Omar Alejandro Murisengo, Silvia Relloso, Matias Vivot, Graciela Davel
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microorganisms in clinical laboratories because it is rapid, relatively simple to use, accurate, and can be used for a wide number of microorganisms. Several studies have demonstrated the utility of this technique in the identification of yeasts; however, its performance is usually improved by the extension of the database. Here we developed an in-house database of 143 strains belonging to 42 yeast species in the MALDI Biotyper platform, and we validated the extended database with 388 regional strains and 15 reference strains belonging to 55 yeast species...
May 11, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29755726/-trueperella-pyogenes-and-brucella-abortus-coinfection-in-a-dog-and-a-cat-on-a-dairy-farm-in-egypt-with-recurrent-cases-of-mastitis-and-abortion
#12
Gamal Wareth, Mohamed El-Diasty, Falk Melzer, Jayaseelan Murugaiyan, Amir Abdulmawjood, Lisa D Sprague, Heinrich Neubauer
Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus . Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based on cpn 60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation of Trueperella pyogenes on the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt...
2018: Veterinary Medicine International
https://www.readbyqxmd.com/read/29752627/maldi-tof-ms-supplementary-database-for-species-identification-employing-the-yeast-diversity-encountered-on-southern-brazil-grapes
#13
Bruna Carla Agustini, Gildo Almeida da Silva, Tania Maria Bordin Bonfim
The study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil...
May 11, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29750765/ideal-and-actual-impact-of-rapid-diagnostic-testing-and-antibiotic-stewardship-on-antibiotic-prescribing-and-clinical-outcomes-in-children-with-positive-blood-cultures
#14
Caroline H Reuter, Hannah L Palac, Larry K Kociolek, Xiaotian T Zheng, Yusuf Y Chao, Rupal Patel, Sameer J Patel
BACKGROUND: Implementing matrix assisted laser desorption ionization time of flight (MALDI-TOF) and multiplex PCR (mPCR) has been associated with decreased mortality and hospital length of stay in adults, but the impact in pediatrics is less understood. METHODS: This pre-post quasi-experimental study compared antibiotic prescribing for positive blood cultures in patients ≤21 years collected in 2012 (pre-intervention) and in 2015 (after MALDI-TOF/mPCR). Time to effective and optimal antimicrobial therapy were evaluated using Cox proportional hazards regression...
May 10, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29749232/towards-spectral-library-free-maldi-tof-ms-bacterial-identification
#15
Ding Cheng, Liang Qiao, Péter Horvatovich
Bacterial identification is of great importance in clinical diagnosis, environmental monitoring and food safety control. Among various strategies, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has drawn significant interests, and has been clinically used. Nevertheless, current bioinformatics solutions use spectral libraries for the identification of bacterial strains. Spectral library generation requires acquisition of MALDI-TOF spectra from monoculture bacterial colonies, which is time-consuming and not possible for many species and strains...
May 11, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29744278/proteomic-identification-of-marker-proteins-and-its-application-to-authenticate-ophiocordyceps-sinensis
#16
Xinxin Tong, Yixuan Wang, Zhengyao Xue, Lu Chen, Yi Qiu, Jing Cao, Cheng Peng, Jinlin Guo
Ophi o cordyceps sinensis ( O. sinensis ) is a highly valuable fungus because of its nutritious and medicinal properties. The objective of this study was to identify protein markers using a proteomics approach followed by the development of an immunoassay to authenticate O. sinensis . Four authentic O. sinensis samples collected from four production regions and four counterfeit samples were examined individually. Overall 22 characteristic proteins of O. sinensis were identified by two-dimensional electrophoresis (2-DE) coupled with the matrix-assisted laser desorption/ionization-time-of-light mass spectrometry (MALDI-TOF/MS)...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29739986/the-influence-of-different-ph-on-the-electrophoretic-behaviour-of-saccharomyces-cerevisiae-modified-by-calcium-ions
#17
Agnieszka Rogowska, Paweł Pomastowski, Michał Złoch, Viorica Railean-Plugaru, Anna Król, Katarzyna Rafińska, Małgorzata Szultka-Młyńska, Bogusław Buszewski
The effect of a different pH on Saccharomyces cerevisiae cells modified with calcium ions was investigated by the capillary zone electrophoresis technique. For the identification of the wild strain of S. cerevisiae, the ribosomal nucleic acid sequencing and internal transcribed spacer sequencing as well as spectrometric approach were applied. The potentiometric titration and Fourier transform infrared spectroscopy have shown the occurrence of active functional groups such as carboxyl, amine/hydroxyl, phosphate/hydrogen phosphate groups on the surface of native yeast cells...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29739926/maldi-tof-ms-for-identification-of-tsukamurella-species-tsukamurella-tyrosinosolvens-as-the-predominant-species-associated-with-ocular-infections
#18
Jade L L Teng, Ying Tang, Samson S Y Wong, Jordan Y H Fong, Zhe Zhao, Chun-Pong Wong, Jonathan H K Chen, Antonio H Y Ngan, Alan K L Wu, Kitty S C Fung, Tak-Lun Que, Susanna K P Lau, Patrick C Y Woo
Although Tsukamurella infections have been increasingly reported in Europe, Asia, America, and Africa, indicating that diseases caused by this group of bacteria are emerging in a global scale, species identification within this genus is difficult in most clinical microbiology laboratories. Recently, we showed that groEL gene sequencing is useful for identification of all existing Tsukamurella species. Nevertheless, PCR sequencing is still considered expensive, time-consuming, and technically demanding, and therefore is yet to be incorporated as a routine identification method in clinical laboratories...
May 9, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29738604/five-year-profile-of-candidemia-at-an-indian-trauma-center-high-rates-of-candida-auris-blood-stream-infections
#19
Purva Mathur, Fahmi Hasan, Pradeep Kumar Singh, Rajesh Malhotra, Kamini Walia, Anuradha Chowdhary
BACKGROUND: Candidemia is a potentially fatal infection with varied distribution of Candida species and their antifungal susceptibility profiles. The recent emergence of Candida auris in invasive candidiasis is a cause for concern. This study describes the profile of candidemia at an Indian tertiary care hospital and reports the emergence of Candida auris. METHODS: All patients diagnosed as having candidemia between 2012 to 2017 were studied. The isolates were identified using conventional methods, VITEK 2 and by the MALDI-TOF MS...
May 8, 2018: Mycoses
https://www.readbyqxmd.com/read/29732635/new-approach-for-fast-identification-of-cyclitols-by-maldi-tof-mass-spectrometry
#20
Hossam Al-Suod, Paweł Pomastowski, Magdalena Ligor, Viorica Railean-Plugaru, Bogusław Buszewski
INTRODUCTION: Alfalfa (Medicago sativa L.) is the subject of many studies due to its numerous chemical constituents and beneficial properties. Among these constituents are cyclitols, which have attracted attention due to the variety of biological properties they have. OBJECTIVE: A rapid and sensitive analytical procedure based on matrix-assisted laser desorption ionisation technique with time-of-flight and mass spectrometry (MALDI-TOF-MS) analysis was used for the first time for the identification of three cyclitols from different parts of alfalfa...
May 6, 2018: Phytochemical Analysis: PCA
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