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Maldi-tof identification

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https://www.readbyqxmd.com/read/28929961/-national-antimicrobial-resistance-surveillance-system-namrss-external-quality-assessment-studies-2011-2016
#1
Serap Süzük Yıldız, Hüsniye Şimşek, Nilay Çöplü, Zeynep Gülay
Establishment of sustainable and evidence-based surveillance systems are recommended for prevention of microbial resistance by the World Health Organization (WHO). As a necessity of these surveillance systems, participants are recommended to implement an external quality assessment (EQA) program. In this scope, National Antimicrobial Resistance Surveillance System (NARSS) has been established within the Public Health Institute of Turkey (PHIT) in our country since 2011. In the scope of this surveillance, NARSS EQA program has been implemented in a cycle per year and four isolates were sent to participants per cycle every year since 2011...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28923505/subcutaneous-infection-by-graphium-basitruncatum-in-a-heart-transplant-patient
#2
Analía L Fernández, Patricia O Andres, Cecilia H Veciño, Claudia B Nagel, María Teresa Mujica
Graphium basitruncatum, a synanamorph of Pseudoallescheria has been rarely reported in human infections. We report a case of subcutaneous phaeohyphomycosis caused by this fungus in a heart transplant recipient. We also describe the phenotypic, molecular methods and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) used to achieve isolate identification.
September 15, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28920176/isolation-and-identification-of-antifungal-peptides-from-bacillus-amyloliquefaciens-w10
#3
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography...
September 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28919552/accurate-identification-of-anopheles-gambiae-giles-trophic-preferences-by-maldi-tof-ms
#4
Sirama Niare, Fatalmoudou Tandina, Bernard Davoust, Ogobara Doumbo, Didier Raoult, Philippe Parola, Lionel Almeras
The determination of the trophic preferences of the Anopheles gambiae Giles (Diptera: Culicidae) is a decisive parameter for the monitoring and the prevention of malaria risk transmission. Currently, arthropod blood feeding sources are identified using immunological or molecular biology traditional techniques. Despite the effectiveness of these methods, they present several limitations, and notably, they are time-consuming and costly techniques. A recent study demonstrated that MALDI-TOF MS could be a useful tool for the identification of blood meal origins in freshly engorged mosquitoes...
September 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28917755/identification-of-trypanosomatids-by-detecting-single-nucleotide-fingerprints-using-dna-analysis-by-dynamic-chemistry-with-maldi-tof
#5
María Angélica Luque-González, Mavys Tabraue-Chávez, Bárbara López-Longarela, Rosario María Sánchez-Martín, Matilde Ortiz-González, Miguel Soriano-Rodríguez, José Antonio García-Salcedo, Salvatore Pernagallo, Juan José Díaz-Mochón
Protozoan parasites of the Trypanosomatidae family can cause devastating diseases in humans and animals, such as Human African Trypanosomiasis or Sleeping Sickness, Chagas disease and Leishmaniasis. Currently, there are molecular assays for detecting parasitic infections and their post-treatment monitoring based on nucleic acid amplification, but there are still certain limitations which limit the development of assays that can detect and discriminate between parasite infections with a single test. Here, we present the development of a novel molecular assay for the rapid identification of Trypanosomatids, integrating DNA analysis by dynamic chemistry in conjunction with Matrix-Assisted Laser Desorption Ionization - Time-of-Flight Mass Spectrometry (MALDI-ToF)...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28900246/maldi-tof-ms-based-identification-and-molecular-characterization-of-food-associated-methicillin-resistant-staphylococcus-aureus
#6
H M Manukumar, S Umesha
Food-borne methicillin resistant Staphylococcus aureus (MRSA) is involved in two-fold higher mortality rate compared to methicillin susceptible S. aureus (MSSA). Eventhough Mysuru recognized as cleanest city in the world, prevalence of food contamination is not detailed. The aim is to screen food samples from Mysuru area and to characterize MRSA strain, employing MALDI-Biotyper, multiplex PCR to distinguish between MRSA and MSSA by PCR-coupled single strand conformation polymorphism (PCR-SSCP). Of all the food-borne pathogens, S...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899453/application-of-maldi-tof-ms-systems-in-the-rapid-identification-of-campylobacter-spp-of-public-health-importance
#7
Ying-Hsin Hsieh, Yun F Wang, Hercules Moura, Nancy Miranda, Steven Simpson, Ramnath Gowrishankar, John Barr, Khalil Kerdahi, Irshad M Sulaiman
Campylobacteriosis is an infectious gastrointestinal disease caused by Campylobacter spp.In most cases, it is either underdiagnosed or underreported due to poor diagnostics and limited databases. Several DNA-based molecular diagnostic techniques, including 16S ribosomal RNA (rRNA) sequence typing, have been widely used in the species identification of Campylobacter. Nevertheless, these assays are time-consuming and require a high quality of bacterial DNA. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS is an emerging diagnostic technology that can provide the rapid identification of microorganisms by using their intact cells without extraction or purification...
September 12, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28890887/isolation-and-characterization-of-actinobacteria-from-algerian-sahara-soils-with-antimicrobial-activities
#8
Harir Mohamed, Bellahcene Miloud, Fortas Zohra, José María García-Arenzana, Antonio Veloso, Susana Rodríguez-Couto
Extreme ecosystems can be a source of untapped microorganisms to produce novel bioactive compounds of industrial interest. Consequently, in this work, 32 actinomycetes were isolated from 6 soil samples collected from Algerian Sahara in searching for untapped producers of novel antimicrobial compounds. All the isolates were further subjected to antimicrobial screening against pathogenic bacteria, yeast and fungi. The obtained results indicated that three of the isolates (named C, MS1 and 10) showed antimicrobial activities against most of the tested pathogenic microorganisms...
2017: International Journal of Molecular and Cellular Medicine
https://www.readbyqxmd.com/read/28889758/maldi-tof-ms-versus-16s-rrna-sequencing-minor-discrepancy-between-tools-in-identification-of-bacteroides-isolates
#9
Károly Péter Sárvári, József Sóki, Miklós Iván, Cecilia Miszti, Krisztina Latkóczy, Szilvia Zsóka Melegh, Edit Urbán
Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of these tests...
September 11, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28884851/-bottom-up-in-situ-proteomic-differentiation-of-human-and-non-human-haemoglobins-for-forensic-purposes-by-maldi-tof-ms-ms
#10
K P Kirkbride, S Kamanna, J Henry, N Voelcker, A Linacre
RATIONALE: The detection and identification of human blood on crime-related items are of particular relevance to many investigations because shed blood can provide evidence of violent contact between individuals. However, for any detection and identification technique, specificity is a critical performance characteristic to assess; that is, whether the technique has the capability to differentiate between human blood (which usually is of relevance to a criminal investigation) and non-human blood (which usually would not be associated with a crime but may be detected incidentally)...
September 8, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28877672/chronic-lower-extremity-wound-infection-due-to-kerstersia-gyiorum-in-a-patient-with-buerger-s-disease-a-case-report
#11
Irmak Baran, Arife Polat Düzgün, İpek Mumcuoğlu, Neriman Aksu
BACKGROUND: Kerstersia gyiorum is an extremely rare pathogen of human infection. It can cause chronic infection in patients with underlying conditions. It can easily be misdiagnosed if proper diagnostic methods are not used. CASE PRESENTATION: A 47-year-old male patient with a history of Buerger's Disease for 28 years presented to our hospital with an infected chronic wound on foot. The wound was debrided, and the specimen was sent to Microbiology laboratory. Gram staining of the specimen showed abundant polymorphonuclear leukocytes and gram-negative bacilli...
September 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28877205/identification-of-shiga-toxigenic-escherichia-coli-outbreak-isolates-by-a-novel-data-analysis-tool-after-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#12
Martin Christner, Dirk Dressler, Mark Andrian, Claudia Reule, Orlando Petrini
The fast and reliable characterization of bacterial and fungal pathogens plays an important role in infectious disease control and tracking of outbreak agents. DNA based methods are the gold standard for epidemiological investigations, but they are still comparatively expensive and time-consuming. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable and cost-effective technique now routinely used to identify clinically relevant human pathogens. It has been used for subspecies differentiation and typing, but its use for epidemiological tasks, e...
2017: PloS One
https://www.readbyqxmd.com/read/28876034/-purification-characterization-and-partial-primary-structure-analysis-of-rutin-degrading-enzyme-in-tartary-buckwheat-seeds
#13
Yuwei Zhang, Jie Li, Yong Yuan, Jijuan Gu, Peng Chen
Rutin-degrading enzymes (RDE) can degrade rutin into poorly water soluble compound, quercetin, and cause the bitter taste in tartary buckwheat. In the present study RDE from Yu 6-21 tartary buckwheat seeds was purified by ammonium sulphate precipitation, followed by hydrophobic interaction chromatography on Phenyl Sepharose CL-4B, ion exchange chromatography on CM-Cellulose and gel filtration chromatography on Sephadex G-150. Purified RDE showed single band with molecular weight of 66 kDa on SDS-PAGE. The optimum pH and temperature of RDE were 5...
May 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28866114/traditional-approaches-versus-mass-spectrometry-in-bacterial-identification-and-typing
#14
REVIEW
Angela Sloan, Gehua Wang, Keding Cheng
Biochemical methods such as metabolite testing and serotyping are traditionally used in clinical microbiology laboratories to identify and categorize microorganisms. Due to the large variety of bacteria, identifying representative metabolites is tedious, while raising high-quality antisera or antibodies unique to specific biomarkers used in serotyping is very challenging, sometimes even impossible. Although serotyping is a certified approach for differentiating bacteria such as E. coli and Salmonella at the subspecies level, the method is tedious, laborious, and not practical during an infectious disease outbreak...
October 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28859120/semi-quantitative-maldi-tof-for-antimicrobial-susceptibility-testing-in-staphylococcus-aureus
#15
Tucker Maxson, Cheryl L Taylor-Howell, Timothy D Minogue
Antibiotic resistant bacterial infections are a significant problem in the healthcare setting, in many cases requiring the rapid administration of appropriate and effective antibiotic therapy. Diagnostic assays capable of quickly and accurately determining the pathogen resistance profile are therefore crucial to initiate or modify care. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a standard method for species identification in many clinical microbiology laboratories and is well positioned to be applied towards antimicrobial susceptibility testing...
2017: PloS One
https://www.readbyqxmd.com/read/28856620/identification-and-quantification-of-n-acyl-homoserine-lactones-involved-in-bacterial-communication-by-small-scale-synthesis-of-internal-standards-and-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#16
Jan Leipert, Christian Treitz, Matthias Leippe, Andreas Tholey
N-acyl homoserine lactones (AHL) are small signal molecules involved in the quorum sensing of many gram-negative bacteria, and play an important role in biofilm formation and pathogenesis. Present analytical methods for identification and quantification of AHL require time-consuming sample preparation steps and are hampered by the lack of appropriate standards. By aiming at a fast and straightforward method for AHL analytics, we investigated the applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
August 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28856505/comparative-analysis-of-constitutive-proteome-between-resistant-and-susceptible-tomato-genotypes-regarding-to-late-blight
#17
Bruno Soares Laurindo, Renata Dias Freitas Laurindo, Patrícia Pereira Fontes, Camilo Elber Vital, Fábio Teixeira Delazari, Maria Cristina Baracat-Pereira, Derly José Henriques da Silva
Late blight is one of the most destructive diseases of the tomato, resulting in substantial economic losses. There is difficulty in controlling this disease, so the molecular characterization of tomato genotypes may help in the selection of higher resistance tomato plants against Phytophthora infestans, late blight's pathogen. The objective was to analyze the differences with regard to the constitutive proteome between the access Vegetable Germplasm Bank (BGH)-2127, resistant genotype, and Santa Clara-susceptible genotype to late blight...
August 30, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28856455/revisiting-the-taxonomy-of-the-genus-elizabethkingia-using-whole-genome-sequencing-optical-mapping-and-maldi-tof-along-with-proposal-of-three-novel-elizabethkingia-species-elizabethkingia-bruuniana-sp-nov-elizabethkingia-ursingii-sp-nov-and-elizabethkingia
#18
Ainsley C Nicholson, Christopher A Gulvik, Anne M Whitney, Ben W Humrighouse, James Graziano, Brian Emery, Melissa Bell, Vladimir Loparev, Phalasy Juieng, Jarrett Gartin, Chantal Bizet, Dominique Clermont, Alexis Criscuolo, Sylvain Brisse, John R McQuiston
The genus Elizabethkingia is genetically heterogeneous, and the phenotypic similarities between recognized species pose challenges in correct identification of clinically derived isolates. In addition to the type species Elizabethkingia meningoseptica, and more recently proposed Elizabethkingia miricola, Elizabethkingia anophelis and Elizabethkingia endophytica, four genomospecies have long been recognized. By comparing historic DNA-DNA hybridization results with whole genome sequences, optical maps, and MALDI-TOF mass spectra on a large and diverse set of strains, we propose a comprehensive taxonomic revision of this genus...
August 30, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28855303/matrix-assisted-desorption-ionization-time-of-flight-mass-spectrometry-for-the-use-with-positive-blood-cultures-methodology-performance-and-optimization
#19
Matthew L Faron, Blake W Buchan, Nathan A Ledeboer
The early initiation of effective antibiotics for septic patients is essential for patient outcome. Matrix assisted desorption ionization time of flight mass spectrometry (MADLI-TOF MS) has revolutionized clinical microbiology for isolate identification and has the possibility to impact how blood culture testing is performed. This review discusses the various methods to use MALDI-TOF MS for the identification and susceptibility testing of positive blood culture, the performance of these methods, and the outcomes involved with implementation...
August 30, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28854753/proteomic-identification-of-turkey-meleagris-gallopavo-seminal-plasma-proteins
#20
M Slowinska, J Nynca, G J Arnold, T Fröhlich, J Jankowski, K Kozlowski, A Mostek, A Ciereszko
SDS-PAGE combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 2-dimensional electrophoresis (2DE) combined with matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (MALDI TOF/TOF) were applied to characterize the turkey seminal plasma proteome. LC-MS/MS led to the identification of 175 proteins, which were classified according to their function and to corresponding biochemical pathways. Using 2DE and MALDI TOF/TOF, 34 different turkey seminal plasma proteins could be identified, of which 20 were found in more than one spot, indicating different proteoforms of these proteins...
September 1, 2017: Poultry Science
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