keyword
MENU ▼
Read by QxMD icon Read
search

Maldi-tof identification

keyword
https://www.readbyqxmd.com/read/28722283/comparative-analysis-of-storage-conditions-and-homogenization-methods-for-tick-and-flea-species-for-identification-by-maldi-tof-ms
#1
A Nebbak, B El Hamzaoui, J-M Berenger, I Bitam, D Raoult, L Almeras, P Parola
Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months...
July 19, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28711436/a-simple-method-for-rapid-microbial-identification-from-positive-monomicrobial-blood-culture-bottles-through-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#2
Jung-Fu Lin, Mao-Cheng Ge, Tsui-Ping Liu, Shih-Cheng Chang, Jang-Jih Lu
BACKGROUND AND PURPOSE: Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. METHODS: We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis...
June 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28706514/evaluation-of-the-bruker-biotyper-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-identification-of-aspergillus-species-directly-from-growth-on-solid-agar-media
#3
Ying Li, He Wang, Yu-Pei Zhao, Ying-Chun Xu, Po-Ren Hsueh
We evaluated the accuracy of the Bruker Biotyper matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) system at identifying clinical isolates of Aspergillus species that were grown on agar media. A total of 381 non-duplicate Aspergillus isolates representing 21 different Aspergillus species identified by molecular analysis were included in this study. The Bruker Biotyper MALDI-TOF MS system was able to identify 30.2% (115/381) of the isolates to the species level (score values of ≥2...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28693412/proteomics-based-identification-of-differentially-abundant-proteins-reveals-adaptation-mechanisms-of-xanthomonas-citri-subsp-citri-during-citrus-sinensis-infection
#4
Leandro M Moreira, Márcia R Soares, Agda P Facincani, Cristiano B Ferreira, Rafael M Ferreira, Maria I T Ferro, Fábio C Gozzo, Érica B Felestrino, Renata A B Assis, Camila Carrião M Garcia, João C Setubal, Jesus A Ferro, Julio C F de Oliveira
BACKGROUND: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. RESULTS: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins...
July 11, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28692323/identifying-inhibitors-of-inflammation-a-novel-high-throughput-maldi-tof-screening-assay-for-salt-inducible-kinases-siks
#5
Rachel E Heap, Anthony G Hope, Lesley-Anne Pearson, Kathleen M S E Reyskens, Stuart P McElroy, C James Hastie, David W Porter, J Simon C Arthur, David W Gray, Matthias Trost
Matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry has become a promising alternative for high-throughput drug discovery as new instruments offer high speed, flexibility and sensitivity, and the ability to measure physiological substrates label free. Here we developed and applied high-throughput MALDI TOF mass spectrometry to identify inhibitors of the salt-inducible kinase (SIK) family, which are interesting drug targets in the field of inflammatory disease as they control production of the anti-inflammatory cytokine interleukin-10 (IL-10) in macrophages...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28688366/differentiation-of-flavobacterium-psychrophilum-from-flavobacterium-psychrophilum-like-species-by-maldi-tof-mass-spectrometry
#6
Marta Pérez-Sancho, Ana Isabel Vela, Tom Wiklund, Markus Kostrzewa, Lucas Domínguez, José Francisco Fernández-Garayzábal
Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific identification of F...
June 29, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28683725/maldi-tof-ms-analysis-in-discovery-and-identification-of-serum-proteomic-patterns-of-ovarian-cancer
#7
Agata Swiatly, Agnieszka Horala, Joanna Hajduk, Jan Matysiak, Ewa Nowak-Markwitz, Zenon J Kokot
BACKGROUND: Due to high mortality and lack of efficient screening, new tools for ovarian cancer (OC) diagnosis are urgently needed. To broaden the knowledge on the pathological processes that occur during ovarian cancer tumorigenesis, protein-peptide profiling was proposed. METHODS: Serum proteomic patterns in samples from OC patients were obtained using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Eighty nine serum samples (44 ovarian cancer and 45 healthy controls) were pretreated using solid-phase extraction method...
July 6, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28683525/rapid-identification-of-vibrio-species-isolated-from-the-southern-coastal-regions-of-korea-by-maldi-tof-mass-spectrometry-and-comparison-of-maldi-sample-preparation-methods
#8
Youngjae Cho, Eiseul Kim, Sun-Kyung Han, Seung-Min Yang, Mi-Ju Kim, Hyun-Joong Kim, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Vibrio species are generally recognized as pathogens predominant in seafood along coastal areas. The food industry has sought to develop efficient microbial detection methods. Due to the limits of conventional methods, this study aimed to establish a rapid identification method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were compared to determine the appropriate means to pre-treat Vibrio species, using 17 isolates and five reference strains...
July 7, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28681820/changing-epidemiology-of-coagulase-negative-staphylococci-in-normal-flora-of-skin
#9
Vikas Gautam, Nandini Sethuraman, Ramanpreet Kaur, Suchet Sachdev, Neelam Marwaha, Pallab Ray
Coagulase negative staphylococci (CoNS) have recently emerged as important pathogens causing nosocomial blood stream infections. To evaluate the prevalence of CoNS in cutaneous normal flora, skin swabs were collected from voluntary blood donors and processed for culture and identification using matrix assisted laser desorption ionisation-time of flight (MALDI-TOF). CoNS were isolated from 96% of blood donors, most commonly Staphylococcus hominis (86%), followed by Staphylococcus epidermidis (22%) and Staphylococcus haemolyticus (9%)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28679319/a-new-multi-analytical-approach-for-the-identification-of-synthetic-and-natural-dyes-mixtures-the-case-of-orcein-mauveine-mixture-in-a-historical-dress-of-a-sicilian-noblewoman-of-nineteenth%C3%A2-century
#10
Ilaria Serafini, Livia Lombardi, Claudia Fasolato, Manuel Sergi, Francesca Di Ottavio, Fabio Sciubba, Camilla Montesano, Marcella Guiso, Rita Costanza, Lucia Nucci, Roberta Curini, Paolo Postorino, Maurizio Bruno, Armandodoriano Bianco
In this paper, the application of a multi-analytical approach for the characterisation of synthetic and natural dyes in a historical textile is presented. The work is focused on a historical dress of a Sicilian noblewoman, dating from about 1865-1870. Firstly, SERS on fibre was performed, in order to individuate the classes of dyes employed. The SERS spectra suggested the presence of two main dyes: mauveine and orcein. In order to confirm these preliminary results, two different extraction protocols were applied...
July 5, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28668708/a-case-of-severe-empyema-with-acute-respiratory-distress-syndrome-caused-by-slackia-exigua-requiring-veno-venous-extracorporeal-membrane-oxygenation
#11
M Y Man, H P Shum, Alan Wu, R A Lee, W W Yan
Slackia exigua (S. exigua) is an obligatory anaerobic coccobacillus under the family of Coriobacteriaceae. It is a rare cause of pyogenic extraoral infections. We report a 58-year-old lady with good past health presented with fulminant community-acquired pneumonia causing acute respiratory distress syndrome caused by S. exigua requiring veno-venous extra-corporeal membrane oxygenation (VV-ECMO). Bacterial identification can be challenging and often require 16 S rRNA and MALDI-TOF MS. The patient was treated with amoxicillin-clavulanic acid according to sensitivity and made significant recovery...
June 28, 2017: Anaerobe
https://www.readbyqxmd.com/read/28668254/corrigendum-to-maldi-tof-ms-is-more-accurate-than-vitek-ii-anc-card-and-api-rapid-id-32-a-system-for-the-identification-of-clostridium-species-anaerobe-40-2016-73-75
#12
Young Jin Kim, Si Hyun Kim, Hyun-Jung Park, Hae-Geun Park, Dongchul Park, Sae Am Song, Hee Joo Lee, Dongeun Yong, Jun Yong Choi, Joong-Ki Kook, Hye Ran Kim, Jeong Hwan Shin
No abstract text is available yet for this article.
June 28, 2017: Anaerobe
https://www.readbyqxmd.com/read/28666229/structural-characterization-and-identification-of-cyclic-lipopeptides-produced-by-bacillus-methylotrophicus-dcs1-strain
#13
Nawel Jemil, Angeles Manresa, Francesc Rabanal, Hanen Ben Ayed, Noomen Hmidet, Moncef Nasri
Bacillus methylotrophicus DCS1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. The structural characterization of the isolated lipopeptides was studied by a variety of analytical techniques. The organic extract of DCS1 lipopeptides was fractionated by silica gel column chromatography (60Mesh). Fractions containing lipopeptides were collected and identified by tandem mass spectrometry MALDI-TOF-MS and MALDI-TOF MS(2)...
June 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28664520/identification-of-cyst-wall-proteins-of-the-hypotrich-ciliate-euplotes-encysticus-using-a-proteomics-approach
#14
Bangzheng Wang, Tao Niu, Muhammad Zeeshan Bhatti, Fenfen Chen, Lin Wu, Jiwu Chen
Euplotes encysticus is a species of Hypotrich ciliates, which form cyst wall by secreting the special substances on encounter of adverse environment. It has critical significance to study the component and mechanism underlying resting cyst, during resisting unfavorable conditions in dormancy induction. The present study was aimed to investigate the effects of cyst wall proteins of Euplotes encysticus by using biochemical methods. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28659318/reliable-detection-of-group-b-streptococcus-in-the-clinical-laboratory
#15
Manuel Rosa-Fraile, Barbara Spellerberg
Group B Streptococcus (GBS) is a leading cause of invasive neonatal infections and a significant pathogen in immunocompromised adults. Screening to detect GBS colonization in pregnant women determines the need for antibiotic prophylaxis in that pregnancy. Efficient determination of the GBS colonization status in pregnant woman is crucial. Methods that maximize the probability of GBS recovery are needed. The availability of technologies such as MALDI-TOF/MS, molecular techniques and chromogenic culture media including Granada-type have changed the scenario of GBS detection and identification...
June 28, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28657416/maldi-tof-mass-spectrometry-provides-high-accuracy-in-identification-of-salmonella-at-species-level-but-is-limited-to-type-or-subtype-salmonella-serovars
#16
Lin Kang, Nan Li, Ping Li, Yang Zhou, Shan Gao, Hongwei Gao, Wenwen Xin, Jinglin Wang
Salmonella can cause global foodborne illnesses in humans and many animals. The current diagnostic gold standard used for detecting Salmonella infection is microbiological culture followed by serological confirmation tests. However, these methods are complicated and time-consuming. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis offers some advantages in rapid identification, for example, simple and fast sample preparation, fast and automated measurement, and robust and reliable identification up to genus and species levels, possibly even to the strain level...
April 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/28656386/performance-of-microbial-identification-by-maldi-tof-ms-and-susceptibility-testing-by-vitek-2-from-positive-blood-cultures-after-minimal-incubation-on-solid-media
#17
S L Mitchell, K Alby
Bloodstream infections (BSIs) are a leading cause of patient morbidity and mortality. Rapid identification of organisms from BSIs is critical for initiating targeted antimicrobial therapy. Although many methods exist for rapid identification, they do not provide detailed or definitive susceptibility information. We assessed the utility of both the VITEK MS and Bruker Biotyper MALDI-TOF mass spectrometers to identify organisms from a positive blood culture bottle after only 4 h of growth on solid media compared to identification from overnight growth using the VITEK MS...
June 28, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28652785/prevalence-and-antibiotic-resistance-of-coagulase-negative-staphylococci-isolated-from-poultry-farms-in-three-regions-of-ghana
#18
Vivian Etsiapa Boamah, Christian Agyare, Hayford Odoi, Francis Adu, Stephen Yao Gbedema, Anders Dalsgaard
The use of antibiotics in animal production has been associated with the development and spread of antibiotic-resistant organisms including commensals. Coagulase-negative Staphylococcus (CoNS) species, which were until recently considered non-pathogenic, have been associated with opportunistic infections and high resistance to several antibiotics. This study sought to determine the prevalence, identity, and phenotypic resistance of coagulase-negative Staphylococcus spp. isolated from some selected poultry farms and farm workers in the Ashanti, Brong Ahafo, and Greater Accra regions of Ghana...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28646681/proteomic-analysis-of-developing-wheat-grains-infected-by-powdery-mildew-blumeria-graminis-f-sp-tritici
#19
Jie Li, Xi-Wen Yang, Yong-Chun Li, Ji-Shan Niu, De-Xian He
Blumeria graminis f.sp. tritici (Bgt) infection greatly interferes with the normal source-sink relationships and always causes tremendous loss of yield and quality in wheat. To better understand the impact of this pathogen on grain development, proteome characterization during grain development in susceptible wheat cultivar Xinong 979 infected by powdery mildew was investigated by 2-DE and tandem MALDI-TOF/TOF-MS. Identification of 111 differentially expressed protein spots representing 85 unique proteins and six expression patterns showed a chronological description of wheat grain formation...
June 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28643898/identification-of-cross-reactive-b-cell-epitopes-between-bos-d-9-0101-bos-taurus-and-gly-m-5-0101-glycine-max-by-epitope-mapping-maldi-tof-ms
#20
Ángela María Candreva, Mario Ferrer-Navarro, Silvia Bronsoms, Alejandra Quiroga, Renata Curciarello, Ana Cauerhff, Silvana Petruccelli, Guillermo Horacio Docena, Sebastián Alejandro Trejo
Exposure to cow's milk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion of milk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described between soy and milk proteins . We have previously identified several cross-reactive allergens between milk and soy that may explain this intolerance. The purpose of the present work was to identify epitopes in the purified αS1-casein and the recombinant soy allergen Gly m 5...
June 23, 2017: Proteomics
keyword
keyword
3576
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"