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Maldi-tof identification

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https://www.readbyqxmd.com/read/29331241/evaluation-of-maldi-tof-mass-spectrometry-for-the-identification-of-bacteria-growing-as-biofilms
#1
A M Gaudreau, J Labrie, C Goetz, S Dufour, M Jacques
We evaluated MALDI-TOF mass spectrometry to identify bacteria from biofilms. We compared three sample preparation procedures on biofilms grown in vitro. The extended direct transfer method was able to identify 13 isolates out of 18 (72%) at the species level and 15 out of 18 (83%) at the genus level.
January 10, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29327112/simultaneous-pre-concentration-and-separation-on-simple-paper-based-analytical-device-for-protein-analysis
#2
Ji-Cheng Niu, Ting Zhou, Li-Li Niu, Zhen-Sheng Xie, Fang Fang, Fu-Quan Yang, Zhi-Yong Wu
In this work, fast isoelectric focusing (IEF) was successfully implemented on an open paper fluidic channel for simultaneous concentration and separation of proteins from complex matrix. With this simple device, IEF can be finished in 10 min with a resolution of 0.03 pH units and concentration factor of 10, as estimated by color model proteins by smartphone-based colorimetric detection. Fast detection of albumin from human serum and glycated hemoglobin (HBA1c) from blood cell was demonstrated. In addition, off-line identification of the model proteins from the IEF fractions with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was also shown...
January 11, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29326010/genomics-of-corynebacterium-striatum-an-emerging-multi-drug-resistant-pathogen-of-immunocompromised-patients
#3
Kathleen Nudel, Xiaomin Zhao, Sankha Basu, Xiaoxi Dong, Maria Hoffmann, Michael Feldgarden, Marc Allard, Michael Klompas, Lynn Bry
OBJECTIVES: Corynebacterium striatum is an emerging multi-drug resistant (MDR) pathogen of immunocompromised and chronically ill patients. The objective of these studies was to provide a detailed genomic analysis of disease causing C. striatum and determine the genomic drivers of resistance and resistance-gene transmission. METHODS: A multi-institutional and prospective pathogen genomics program flagged seven MDR C. striatum infections occurring close in time, and specifically in immunocompromised patients with underlying respiratory diseases...
January 8, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29325875/fatal-thoracic-empyema-involving-campylobacter-rectus-a-case-report
#4
A Noël, A Verroken, L Belkhir, H Rodriguez-Villalobos
We report the case of a 69-year-old man admitted for septic shock secondary to necrotic pneumoniae complicated by thoracic empyema of fatal issue. Microbiological examination of pleural liquid revealed a mixed anaerobic flora involving Campylobacter rectus and Actinomyces meyeri. Campylobacter rectus is an infrequent anaerobic pathogen of oral origin To our knowledge, this is the first case report of fatal C. rectus - associated thoracic empyema, and only the second reported case in which identification was successfully performed by MALDI-TOF MS...
January 8, 2018: Anaerobe
https://www.readbyqxmd.com/read/29317173/identification-of-a-proteomic-biomarker-associated-with-invasive-st1-serotype-vi-group-b-streptococcus-by-maldi-tof-ms
#5
Hsiao-Chuan Lin, Jang-Jih Lu, Lee-Chung Lin, Cheng-Mao Ho, Kao-Pin Hwang, Yu-Ching Liu, Chao-Jung Chen
BACKGROUND: Group B Streptococcus (GBS) is an important invasive pathogen in neonates, pregnant women and the elderly. Serotype VI GBS, which has been rarely reported globally, has emerged as a significant pathogen in Asia. However, traditional serologic latex agglutination (LA) methods may fail to type isolates that lack of or low expression of CPS. METHODS: A total of 104 GBS strains were analyzed by MALDI-TOF MS. Multiplex PCR and multilocus sequence typing (MLST) were also performed to confirm their strains...
December 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29314679/molecular-epidemiology-of-trichophyton-quinckeanum-a-zoophilic-dermatophyte-on-the-rise
#6
Silke Uhrlaß, Wieland Schroedl, Carolin Mehlhorn, Constanze Krüger, Vit Hubka, Thomas Maier, Yvonne Gräser, Uwe Paasch, Pietro Nenoff
BACKGROUND: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. PATIENTS AND METHODS: During routine diagnostic tests conducted at the Mycology Laboratory, Mölbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1α, and 3) in some cases, MALDI-TOF mass spectrometry...
January 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/29310582/identification-of-uncommon-oral-yeasts-from-cancer-patients-by-maldi-tof-mass-spectrometry
#7
Narges Aslani, Ghasem Janbabaei, Mahdi Abastabar, Jacques F Meis, Mahasti Babaeian, Sadegh Khodavaisy, Teun Boekhout, Hamid Badali
BACKGROUND: Opportunistic infections due to Candida species occur frequently in cancer patients because of their inherent immunosuppression. The aim of the present study was to investigate the epidemiology of yeast species from the oral cavity of patients during treatment for oncological and haematological malignancies. METHODS: MALDI-TOF was performed to identify yeasts isolated from the oral cavity of 350 cancer patients. Moreover, antifungal susceptibility testing was performed in according to CLSI guidelines (M27-A3)...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29301570/maldi-tof-ms-as-a-new-tool-for-the-identification-of-dientamoeba-fragilis
#8
Adriana Calderaro, Mirko Buttrini, Sara Montecchini, Sabina Rossi, Giovanna Piccolo, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto
BACKGROUND: In this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spectrum and 14 clinical isolates to verify the reliability of the obtained results. RESULTS: While 15 peaks were found to be discriminating between the reference strain and the culture medium used, six peaks, observed in all the 14 strains tested, were considered as markers able to identify D...
January 4, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29287094/validation-of-a-novel-automatic-deposition-of-bacteria-and-yeasts-on-maldi-target-for-maldi-tof-ms-based-identification-using-maldi-colonyst-robot
#9
Katerina Chudejova, Michal Bohac, Anna Skalova, Veronika Rotova, Costas C Papagiannitsis, Jana Hanzlickova, Tamara Bergerova, Jaroslav Hrabák
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic acid and by automatic deposition using MALDI Colonyst...
2017: PloS One
https://www.readbyqxmd.com/read/29280153/methods-for-identification-and-differentiation-of-different-shewanella-spp-isolates-for-diagnostic-use
#10
V Jung-Schroers, A Jung, M Ryll, J Bauer, F Teitge, D Steinhagen
Shewanella spp. are Gram-negative, rod-shaped, motile bacteria that are widely distributed in marine and freshwater environments. The bacteria are present in the physiological microflora of fish from temperate waters and are known as fish spoilage species. From clinically healthy fish and from fish with skin ulcerations, Shewanella spp. is regularly isolated, indicating a possible role as fish pathogen. In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI-TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared...
December 27, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29279453/evaluation-of-the-filmarray-blood-culture-identification-panel-in-detecting-pathogenic-microorganisms-from-positive-blood-cultures-the-first-clinical-report-in-japan
#11
Kyoichi Saito, Shiro Endo, Makoto Katsumi, Chiyuki Ishizawa, Yuko Fujikawa, Shinya Inomata, Masahiro Toyokawa, Mitsuo Kaku
  The FilmArray (FA) is a multiplex polymerase chain reaction (PCR)-based desktop microbial detection system. The Blood Culture Identification (BCID) panel is an adaptable panel for the FA, which diagnoses sepsis or/and systemic infections by detecting 14 bacterial species, 4 bacterial genera, 1 bacterial family, 5 yeast species, and 3 antimicrobial resistance genes (mecA, KPC, and vanA/B) from positive blood cultures within 1 hour. We retrospectively evaluated the FA-BCID panel, using 54 positive blood cultures, from which 57 bacteria and 3 yeasts were identified through matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis...
December 26, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29275560/-research-progress-of-maldi-tof-ims-in-biomedicine-and-its-application-prospect-in-forensic-sciences
#12
REVIEW
G H Ren, R H Weng, Y Shi, P Huang, Z D Li, Y Shao, K F Deng, N G Liu, Y J Chen
Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS) can analysis unknown compounds in sections and obtain molecule imaging by scanning biological tissue sections, which has become a powerful tool for the research of biomarker, lipid distribution and drug metabolism, etc. This article reviews the application of this technique in protein identification, clinical application, drug discovery, lipid research and brain injury.
October 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29272955/laribacter-hongkongensis-clinical-presentation-epidemiology-and-treatment-a-review-of-the-literature-and-report-of-the-first-case-in-denmark
#13
Anne Line Engsbro, Karen Leth Nielsen, Mads Hornum, Leif Percival Andersen
BACKGROUND: Laribacter hongkongensis is an emerging pathogen related to gastroenteritis that can cause invasive and even fatal disease. The aim of this review is to describe the clinical presentation, epidemiology, treatment options and implications for the clinical microbiology laboratory. METHODS: We searched Pubmed using the term Laribacter hongkongensis with limitations human and language English, and identified 35 publications with eight reports on human cases...
December 22, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/29261659/assessment-of-maldi-tof-mass-spectrometry-for-filariae-detection-in-aedes-aegypti-mosquitoes
#14
Djamel Tahir, Lionel Almeras, Marie Varloud, Didier Raoult, Bernard Davoust, Philippe Parola
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as an accurate approach for arthropod identification. Preliminary studies have shown that the MALDI-TOF MS was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and Plasmodium parasites, respectively. The aim of the present study was to test the efficiency of MALDI-TOF MS tool in distinguishing protein profiles between uninfected mosquitoes from specimens infected by filarioid helminths...
December 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29259773/identification-of-organic-pigments-in-tattoo-inks-and-permanent-make-ups-using-maldi-tof-mass-spectrometry
#15
Markus Niederer, Urs Hauri, Lydia Kroll, Christopher Hohl
Nowadays, about 12% of the European and 20% of the US population are tattooed. Rising concerns regarding consumer safety, led to legal restrictions on tattoo inks and permanent make-up (PMU) inks. Restrictions also include bans on certain hazardous colourants. Both ink types use organic pigments for colour-giving, plus inorganic pigments for white and black and colour tones. Pigments are only sparingly soluble in common solvents and occur as suspended particles in the ink matrix. Their detection and identification therefore pose a major challenge for laboratories involved in monitoring the legal compliance of tattoo inks and PMUs...
2017: F1000Research
https://www.readbyqxmd.com/read/29248738/identification-of-immature-stages-of-phlebotomine-sand-flies-using-maldi-tof-ms-and-mapping-of-mass-spectra-during-sand-fly-life-cycle
#16
Petr Halada, Kristyna Hlavackova, Vit Dvorak, Petr Volf
The aim of the study was to evaluate the potential of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for the species identification of sand flies at different developmental stages and map changes in their protein profiles during the course of whole life cycle. Specimens of six different species from laboratory colonies at larval and pupal stages were examined using MALDI-TOF MS. The protein profiles of larvae were stable from the L2 to L4 developmental stages and clearly distinguishable at the species level...
December 14, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29240968/monitoring-of-spoilage-associated-microbiota-on-modified-atmosphere-packaged-beef-and-differentiation-of-psychrophilic-and-psychrotrophic-strains
#17
Maik Hilgarth, Jürgen Behr, Rudi F Vogel
AIMS: This study aimed to explore the discriminatory power of MALDI-TOF MS as a high-throughput method to monitor growth dynamics of meat-spoiling bacteria on modified atmosphere packaged (MAP) beef and to differentiate psychrophilic and psychrotrophic spoilage-associated strains. METHODS AND RESULTS: MAP beef steaks were incubated for 21 days at constant 4 and 10 °C. Development of headspace gas composition, pH, CFU and spoilage-associated microbial composition were monitored...
December 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29239754/characterization-of-rhodococcus-sp-a5wh-isolated-from-a-high-altitude-andean-lake-to-unravel-the-survival-strategy-under-lithium-stress
#18
Carolina Belfiore, María V Curia, María E Farías
Lithium (Li) is widely distributed in nature and has several industrial applications. The largest reserves of Li (over 85%) are in the so-called "triangle of lithium" that includes the Salar de Atacama in Chile, Salar de Uyuni in Bolivia and Salar del Hombre Muerto in Argentina. Recently, the use of microorganisms in metal recovery such as copper has increased; however, there is little information about the recovery of lithium. The strain Rhodococcus sp. A5wh used in this work was previously isolated from Laguna Azul...
November 24, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29234137/ratiometric-mass-spectrometry-for-cell-identification-and-quantitation-using-intracellular-dual-biomarkers
#19
Xiaoming Chen, Fangjie Wo, Jiang Chen, Jie Tan, Tao Wang, Xiao Liang, Jianmin Wu
This study proposed an easy-to-use method for cell identification and quantitation by ratiometric matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Two pairs of MS peaks in the molecular fingerprint of cells were selected as intracellular dual-biomarkers due to the stability and specificity of their ratio values in different types of hepatocellular cancer (HCC) cell lines. Five types of HCC cells can be thereafter differentiated based on these two pairs of intracellular peptides/proteins...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233117/elizabethkingia-miricola-as-an-opportunistic-oral-pathogen-associated-with-superinfectious-complications-in-humoral-immunodeficiency-a-case-report
#20
Przemysław Zdziarski, Mariola Paściak, Klaudia Rogala, Agnieszka Korzeniowska-Kowal, Andrzej Gamian
BACKGROUND: Elizabethkingia miricola is a rare Gram-negative bacterium found in water and clinical specimens. Typical culturing methods often misidentify Elizabethkingia spp. as Flavobacterium or Chryseobacterium. Although diagnosis is based on culturing samples taken from sterile sites, such as blood, a proper identification of this bacterium requires an expertise that goes beyond the capabilities of a typical clinical laboratory. CASE PRESENTATION: A 35-year-old woman diagnosed with common variable immunodeficiency was admitted to our center...
December 12, 2017: BMC Infectious Diseases
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