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Maldi-tof identification

A C M Veloo, H Jean-Pierre, U S Justesen, T Morris, E Urban, I Wybo, M Kostrzewa, A W Friedrich
Within the ENRIA project, several 'expertise laboratories' collaborated in order to optimize the identification of clinical anaerobic isolates by using a widely available platform, the Biotyper Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Main Spectral Profiles (MSPs) of well characterized anaerobic strains were added to one of the latest updates of the Biotyper database db6903; (V6 database) for common use. MSPs of anaerobic strains nominated for addition to the Biotyper database are included in this validation...
March 12, 2018: Anaerobe
Jiekai Yu, Yanqin Huang, Chen Lin, Xiaofen Li, Xuefeng Fang, Chenhan Zhong, Ying Yuan, Shu Zheng
The serum protein markers of colorectal adenoma in patients with a family history of colorectal cancer have been rarely reported. Serum samples from colorectal adenoma patients with or without a family history of colorectal cancer and healthy controls were profiled using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). The model to distinguish colorectal adenoma patients with a family history of colorectal cancer from atypical hereditary colorectal families (CRA-H) and sporadic colorectal adenoma patients without a family history of colorectal cancer (CRA-S) was established with 85...
2018: Journal of Cancer
Amy N Jacobson, Biswa P Choudhury, Michael A Fischbach
Lipopolysaccharide (LPS), a cell-associated glycolipid that makes up the outer leaflet of the outer membrane of Gram-negative bacteria, is a canonical mediator of microbe-host interactions. The most prevalent Gram-negative gut bacterial taxon, Bacteroides , makes up around 50% of the cells in a typical Western gut; these cells harbor ~300 mg of LPS, making it one of the highest-abundance molecules in the intestine. As a starting point for understanding the biological function of Bacteroides LPS, we have identified genes in Bacteroides thetaiotaomicron VPI 5482 involved in the biosynthesis of its lipid A core and glycan, generated mutants that elaborate altered forms of LPS, and used matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry to interrogate the molecular features of these variants...
March 13, 2018: MBio
Noriko Takeuchi, Shunsuke Segawa, Naruhiko Ishiwada, Misako Ohkusu, Sachio Tsuchida, Mamoru Satoh, Kazuyuki Matsushita, Fumio Nomura
Haemophilus influenzae is a major pathogenic bacteria causing invasive disease, which is classified into six capsular serotypes (a-f) and non-typeable (NT) strains. Capsular serotyping of H. influenzae is traditionally determined by serological methods and more recently by PCR methods. However, these methods are time-consuming and expensive. In the present study, matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated as an alternative method for capsular serotyping of H...
March 10, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Massimiliano Magro, Mattia Zaccarin, Giovanni Miotto, Laura Da Dalt, Davide Baratella, Piero Fariselli, Gianfranco Gabai, Fabio Vianello
Surface active maghemite nanoparticles (SAMNs) are able to recognize and bind selected proteins in complex biological systems, forming a hard protein corona. Upon a 5-min incubation in bovine whey from mastitis-affected cows, a significant enrichment of a single peptide characterized by a molecular weight at 4338 Da originated from the proteolysis of aS1 -casein was observed. Notably, among the large number of macromolecules in bovine milk, the detection of this specific peptide can hardly be accomplished by conventional analytical techniques...
March 12, 2018: Analytical and Bioanalytical Chemistry
Samantha J Lawton, Allison M Weis, Barbara A Byrne, Heather Fritz, Conor C Taff, Andrea K Townsend, Bart C Weimer, Asli Mete, Sarah Wheeler, Walter M Boyce
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1)...
March 1, 2018: Journal of Veterinary Diagnostic Investigation
Annie W T Lee, Johnson K S Lam, Ricky K W Lam, Wan H Ng, Ella N L Lee, Vicky T Y Lee, Po P Sze, Rahim Rajwani, Kitty S C Fung, Wing K To, Rodney A Lee, Dominic N C Tsang, Gilman K H Siu
Objective: This study evaluated the capability of a MALDI Biotyper system equipped with the newly introduced MBT STAR-BL module to simultaneously perform species identification and β-lactamase-mediated resistance detection in bacteremia -causing bacteria isolated from cultured isolates and patient-derived blood cultures (BCs). Methods: Two hundred retrospective cultured isolates and 153 prospective BCs containing Gram-negative rods (GNR) were collected and subjected to direct bacterial identification, followed by the measurement of β-lactamase activities against ampicillin, piperacillin, cefotaxime, ceftazidime, and meropenem using the MBT STAR-BL module...
2018: Frontiers in Microbiology
SangWook Lee, Jong Hyun Lee, Hyuck Gi Kwon, Thomas Laurell, Ok Chan Jeong, Soyoun Kim
Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate?specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol?gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Pavel Cejnar, Stepanka Kuckova, Ales Prochazka, Ludmila Karamonova, Barbora Svobodova
RATIONALE: Explorative statistical analysis of mass spectrometry data is still a time-consuming step. We analyzed critical factors for application of principal component analysis in mass spectrometry and focused on two whole spectrum based normalization techniques and their application in analysis of registered peak data and, in comparison, in full spectrum data analysis. We used this technique to identify different metabolic patterns in the bacterial culture of Cronobacter sakazakii, an important foodborne pathogen...
March 9, 2018: Rapid Communications in Mass Spectrometry: RCM
Ludmila Vilela Resende, Leticia Kleinhans Pinheiro, Maria Gabriela da Cruz Pedroso Miguel, Cíntia Lacerda Ramos, Danielle Marques Vilela, Rosane Freitas Schwan
The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemically characterized by gas and liquid chromatography (HPLC and GC-MS). Aerobic mesophilic bacteria (AMB) (35.8% of total of isolated microorganisms), lactic acid bacteria (LAB) (21.6%) and yeast (42...
March 8, 2018: World Journal of Microbiology & Biotechnology
Mazen Alssahen, Osama Sammra, Jörn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Christoph Lämmler, Mirja Raunio Saarnisto, Ellen Prenger-Berninghoff, Markus Timke, Andrè Becker, Amir Abdulmawjood
In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl. The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes...
March 2018: Veterinary Microbiology
Dan-Feng Zhang, Jin-Zhou Ye, Hong-Hou Dai, Xiang-Min Lin, Hui Li, Xuan-Xian Peng
Ethanol is an efficient disinfectant, but long-term and wide usage of ethanol leads to microbial tolerance. Bacteria with the tolerance are widely identified. However, mechanisms of the tolerance are not elucidated. To explore the mechanisms of outer membrane (OM) proteins underlying ethanol tolerance in bacteria, functional proteomic methodologies were utilized to characterize OM proteins of E. coli suddenly exposed to 3.125% ethanol. Of eleven proteins altered significantly, seven were OM proteins, in which LamB, FadL and OmpC were up-regulated, and OmpT, OmpF, Tsx and OmpA were down-regulated...
March 5, 2018: Journal of Proteomics
Yan Cao, Lei Wang, Ping Ma, Wenting Fan, Bing Gu, Shaoqing Ju
Mycobacterium species are a significant cause of morbidity and mortality worldwide. The present study was carried out to systematically evaluate the accuracy of Matrix-assisted laser desorption ionization-time of flight mass spectroscopy (MALDI-TOF MS) for the identification of clinical pathogenic mycobacteria. After a rigid selection process, 19 articles involving 2,593 mycobacteria isolates were included. The pooled result agreed with the reference method identification for 85% of the isolates to genus level, with 71% (95% CI of 69% to 72%) correct to the species level...
March 7, 2018: Scientific Reports
Viktoria I Pauker, Bryan R Thoma, Gregor Grass, Pauline Bleichert, Matthias Hanczaruk, Lothar Zöller, Sabine Zange
Discrimination of highly pathogenic bacteria, such as Bacillus anthracis , from closely related species based on molecular biological methods is challenging. We applied Matrix Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS) to a collection of B. anthracis strains and close relatives in order to significantly improve the statistical confidence of identification results for this group of bacteria. Protein mass spectra of 189 verified and diverse Bacillus strains of the Bacillus cereus sensu lato group were generated using MALDI-TOF MS and subsequently analyzed with supervised and unsupervised statistical methods, such as shrinkage discriminant analysis (SDA) and principle component analysis (PCA)...
March 7, 2018: Journal of Clinical Microbiology
Lucas Eduardo Trevisoli, Larissa Bail, Luiza Souza Rodrigues, Danieli Conte, Jussara Kasuko Palmeiro, Libera Maria Dalla-Costa
INTRODUCTION.: This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS.: A total of 134 CoNS strains were characterized using four different methods. RESULTS.: The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1...
January 2018: Revista da Sociedade Brasileira de Medicina Tropical
Hugo López-Fernández, José E Araújo, Susana Jorge, Daniel Glez-Peña, Miguel Reboiro-Jato, Hugo M Santos, Florentino Fdez-Riverola, José L Capelo
BACKGROUND AND OBJECTIVE: 2D-gel electrophoresis is widely used in combination with MALDI-TOF mass spectrometry in order to analyze the proteome of biological samples. For instance, it can be used to discover proteins that are differentially expressed between two groups (e.g. two disease conditions, case vs. control, etc.) thus obtaining a set of potential biomarkers. This procedure requires a great deal of data processing in order to prepare data for analysis or to merge and integrate data from different sources...
March 2018: Computer Methods and Programs in Biomedicine
Vishakha Pandey, Manoj Singh, Dinesh Pandey, Soma Marla, Anil Kumar
Tilletia indica is a smut fungus that incites Karnal bunt in wheat. It has been considered as quarantine pest in more than 70 countries. Despite its quarantine significance, there is meager knowledge regarding the molecular mechanisms of disease pathogenesis. Moreover, various disease management strategies have proven futile. Development of effective disease management strategy requires identification of pathogenicity/virulence factors. With this aim, the present study was conducted to compare the secretomes of T...
March 6, 2018: Proteomics
D Raszka, S Popelka, J Heřt, D Jahoda, I Landor, P Vavřík
Nocardiosis is a rare infection caused by the aerobic actinomycete of the Nocardia genus. In most cases, nocardiosis manifests as a lung infection or a bone lesion. Due to the nonspecific and mild clinical manifestations of nocardiosis, the establishment of definite diagnosis can be difficult. When antibiotic therapy is incorrectly targeted, only the symptoms of the disease are suppressed. The mainstay in the treatment of Nocardia osteomyelitis has so far been the combined surgical debridement with long-term, initially intravenous, antibiotic administration...
March 5, 2018: Folia Microbiologica
Akira Yokoyama, Hidenori Kage, Yuki Ohama, Tomoaki Sato, Naoya Miyashita, Satoshi Noguchi, Shu Okugawa, Yasuhiro Yamauchi, Kyoji Moriya, Takahide Nagase
Identification of microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely accepted. However, the significance of MALDI-TOF MS for identifying mycobacteria, particularly rare nontuberculous mycobacteria, has not been established. M. heckeshornense is one such bacteria, and distinguishing it from M. xenopi is difficult. The patient was a 40-year-old man with Behҫet's disease who had started treatment with prednisolone and azathioprine. A lung nodule in the right lower lobe was pointed out, and it increased in size 6 months later...
March 1, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Guohong Peng, Björn Meyer, Lucie Sokolova, Wenxia Liu, Sandra Bornemann, Jana Juli, Klaus Zwicker, Michael Karas, Bernd Brutschy, Hartmut Michel
The NADH:ubiquinone oxidoreductase (complex I) is the first enzyme of the respiratory chain and the entry point for most electrons. Generally, the bacterial complex I consists of 14 core subunits, homologues of which are also found in complex I of mitochondria. In complex I preparations from the hyperthermophilic bacterium Aquifex aeolicus we have identified 20 partially homologous subunits by combining MALDI-TOF and LILBID mass spectrometry methods. The subunits could be assigned to two different complex I isoforms, named NQOR1 and NQOR2...
February 28, 2018: Biochimica et Biophysica Acta
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