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Maldi-tof identification

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https://www.readbyqxmd.com/read/29148919/bacterial-flora-analysis-of-coliforms-in-sewage-river-water-and-ground-water-using-maldi-tof-mass-spectrometry
#1
Yoshihiro Suzuki, Kouki Niina, Tomonori Matsuwaki, Kei Nukazawa, Atsushi Iguchi
The aim of this study was to rapidly and effectively analyze coliforms, which are the most fundamental indicators of water quality for fecal pollution, using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Coliform bacteria were isolated from municipal sewage, river water, and groundwater. For each sample, 100 isolates were determined by MALDI-TOF MS. In addition, these same 100 isolates were also identified via 16S rRNA gene sequence analysis. Obtained MALDI-TOF MS data were compared with the 16S rRNA sequencing analysis, and the validity of MALDI-TOF MS for classification of coliform bacteria was examined...
November 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29148742/identification-of-the-peptide-pyroq-%C3%AE-casein194-209-as-a-highly-specific-and-sensitive-marker-to-differentiate-between-ultrahigh-temperature-processed-uht-milk-and-mildly-heated-milk
#2
Sevim Dalabasmaz, Jennifer Ebner, Monika Pischetsrieder
In this study, a new approach was introduced to identify marker peptides that reflect the thermal treatment of commercial milk samples and differentiate ultrahigh-temperature processed (UHT) milk from mildly heated milk. Peptide profiles of training set samples, pasteurized (n=20), extended shelf life (n=29), and UHT (n=29) milk, were recorded by MALDI-TOF-MS after StageTip micro-extraction. As marker candidates, 13 peptides were selected and their cutoff levels were defined. The quality of the cutoff levels was then tested with a blind test set...
November 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29143879/comparison-of-analytical-tools-appropriate-for-identification-of-proteinaceous-additives-in-historical-mortars
#3
Iva Krizova, Julia Schultz, Ivan Nemec, Radomir Cabala, Radovan Hynek, Stepanka Kuckova
Natural organic additives such as eggs, lard, resins, and oils have been added to mortars since ancient times, because the ancient builders knew of their positive effect on the mortar quality. The tradition of adding organic materials to mortars was commonly handed down only verbally for thousands years. However, this practice disappeared in the nineteenth century, when the usage of modern materials started. Today, one of the most recent topics in the industry of building materials is the reusing of natural organic materials and searching for the forgotten ancient recipes...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29142047/multi-center-evaluation-of-the-vitek-ms-v3-0-system-for-the-identification-of-filamentous-fungi
#4
Jenna Rychert, E Sue Slechta, Adam P Barker, Edwin Miranda, N Esther Babady, Yi-Wei Tang, Connie Gibas, Nathan Wiederhold, DeAnna Sutton, Kimberly E Hanson
Invasive fungal infections are an important cause of morbidity and mortality affecting primarily immunocompromised patients. While fungal identification to the species level is critical to providing appropriate therapy, it can be slow, laborious, and often relies on subjective morphologic criteria. The use of MALDI-TOF mass spectrometry has the potential to speed up and improve the accuracy of identification. In this multi-center study, we evaluated the accuracy of the VITEK® MS v3.0 system in identifying 1,601 clinical mold isolates as compared to identification by DNA sequence analysis and supported by morphologic and phenotypic testing...
November 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29138467/bacillus-classification-based-on-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-effects-of-culture-conditions
#5
Lin-Jie Shu, Yu-Liang Yang
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a reliable and rapid technique applied widely in the identification and classification of microbes. MALDI-TOF MS has been used to identify many endospore-forming Bacillus species; however, endospores affect the identification accuracy when using MALDI-TOF MS because they change the protein composition of samples. Since culture conditions directly influence endospore formation and Bacillus growth, in this study we clarified how culture conditions influence the classification of Bacillus species by using MALDI-TOF MS...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29135031/comparative-proteomics-illustrates-the-molecular-mechanism-of-potato-solanum-tuberosum-l-tuberization-inhibited-by-exogenous-gibberellins-in-vitro
#6
Lixiang Cheng, Yuping Wang, Yueshan Liu, Qingquan Zhang, Huihui Gao, Zhang Feng
Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs-signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA3 treatments (0, 0.01, 0.1 and 1.0 μΜ) using two-dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2-fold...
November 14, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29134938/short-term-incubation-of-positive-blood-cultures-in-brain-heart-infusion-broth-accelerates-identification-of-bacteria-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#7
Ignacio Torres, Estela Gimenez, Tania Pascual, Felipe Bueno, Dixie Huntley, Mireia Martínez, David Navarro
PURPOSE: Fast identification of bacteria directly from positive blood cultures (BCs) by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) can be achieved either using the MALDI Sepsityper kit (protein extraction method) or after a short-term pre-cultivation step on solid medium. We developed a new method that involves short-term enrichment of positive BCs in brain-heart infusion broth (BHI) prior to MALDI-TOF MS analysis. METHODOLOGY: Eighty-four BCs flagged as positive were included in this study; these were processed in parallel either directly using the MALDI Sepsityper kit or following a short-term culture either in BHI or on Columbia blood agar with 5 % sheep blood (CBA)...
November 14, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29128765/exploring-the-links-between-groundwater-quality-and-bacterial-communities-near-oil-and-gas-extraction-activities
#8
Inês C Santos, Misty S Martin, Michelle L Reyes, Doug D Carlton, Paula Stigler-Granados, Melissa A Valerio, Kristina W Whitworth, Zacariah L Hildenbrand, Kevin A Schug
Bacterial communities in groundwater are very important as they maintain a balanced biogeochemical environment. When subjected to stressful environments, for example, due to anthropogenic contamination, bacterial communities and their dynamics change. Studying the responses of the groundwater microbiome in the face of environmental changes can add to our growing knowledge of microbial ecology, which can be utilized for the development of novel bioremediation strategies. High-throughput and simpler techniques that allow the real-time study of different microbiomes and their dynamics are necessary, especially when examining larger data sets...
November 9, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29128091/non-protease-native-allergens-partially-purified-from-bodies-of-eight-domestic-mites-using-p-aminobenzamidine-ligand
#9
T Erban, R Klubal
BACKGROUND: Optimised purification steps for concentrating trace target native antigens are needed. Combining the p-aminobenzamidine ligand with protease inactivation enables partial purification of mite non-protease allergens lacking proteases. OBJECTIVE: We sought to analyse in detail proteins obtained using this method from eight species of synanthropic acaridid mites and tested IgE reactivity using pooled human sera. MATERIALS AND METHODS: Proteins affinity bound to p-aminobenzamidine as a ligand were identified by MALDI TOF/TOF...
November 8, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/29123122/identification-of-low-oxygen-tolerating-bacteria-in-prostate-secretions-of-cancer-patients-and-discussion-of-possible-aetiological-significance
#10
Roshni Bhudia, Amar Ahmad, Onyinye Akpenyi, Angela Whiley, Mark Wilks, Tim Oliver
The microaerophylic organism Propionibacterium acnes has shown consistent association with prostate cancer (PC). Studies linking circumcision with reduced PC further support anaerobes involvement as circumcision reduces anaerobe colonisation on the glans penis. A 1988 study linked anaerobes with PC but considered them as opportunists in necrotic tumour. A hypothesis that a "Helicobacter-like" process causes PC justified this pilot study. Active surveillance patients were enrolled. Post-prostate massage urine samples were screened using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) technique for bacterial identification after culture in anaerobic and aerobic conditions...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29118171/total-laboratory-automation-and-maldi-tof-improve-turnaround-times-in-the-clinical-microbiology-laboratory-a-retrospective-analysis
#11
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker MALDI-TOF and BD Kiestra Total Laboratory Automation (TLA) three and four years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TAT) for positive and negative urine cultures before and after implementation. Comparison I: TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF for organism identification...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29113712/prior-antimicrobial-therapy-duration-influences-causative-pathogens-identification-in-ventilator-associated-pneumonia
#12
Jean-François Llitjos, Marlène Amara, Ahlem Benzarti, Guillaume Lacave, Jean-Pierre Bedos, Béatrice Pangon
OBJECTIVE: To determine whether prior antimicrobial therapy, divided in recent or current antibiotic treatment, influences the identification rate and/or the type of causative pathogens in patients with suspected episodes of ventilator-acquired pneumonia. DESIGN: Monocentric retrospective study. SETTING: Intensive car unit in a universitary hospital. PATIENTS: 230 episodes of ventilator-associated pneumonia with a Clinical Pulmonary Infection Score≥6 were retrospectively evaluated...
October 19, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29113600/maldi-tof-ms-protein-profiling-for-the-rapid-identification-of-chagas-disease-triatomine-vectors-and-application-to-the-triatomine-fauna-of-french-guiana-corrigendum
#13
Maureen Laroche, Jean-Michel Bérenger, Gladys Gazelle, Denis Blanchet, Didier Raoult, Philippe Parola
No abstract text is available yet for this article.
November 8, 2017: Parasitology
https://www.readbyqxmd.com/read/29112767/fast-and-reliable-diagnosis-of-xdr-acinetobacter-baumannii-meningitis-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#14
Grazia Brunetti, Giancarlo Ceccarelli, Alessandra Giordano, Anna Sara Navazio, Pietro Vittozzi, Mario Venditti, Giammarco Raponi
Bacterial meningitis is a medical emergency needing quick and timely diagnosis. Even though meningitis caused by Acinetobacter baumannii is relatively rare, it is associated with high mortality rates especially in neurosurgery patients and represents a serious therapeutic problem due to the limited penetration of effective antibiotics into the cerebrospinal fluid. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) has been effectively used as a rapid method for microbial identification...
November 7, 2017: New Microbiologica
https://www.readbyqxmd.com/read/29112122/identification-and-antimicrobial-susceptibility-testing-of-anaerobic-bacteria-rubik-s-cube-of-clinical-microbiology
#15
REVIEW
Márió Gajdács, Gabriella Spengler, Edit Urbán
Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically...
November 7, 2017: Antibiotics
https://www.readbyqxmd.com/read/29111606/incubation-with-cu-ii-and-zn-ii-salts-enhances-maldi-tof-mass-spectra-of-amyloid-beta-and-%C3%AE-synuclein-toward-in-vivo-analysis
#16
Andrea R Kelley, Madeline E Colley, George Perry, Stephan B H Bach
Insoluble senile plaque aggregates are indicative of Alzheimer's disease (AD) pathology. A similar phenomenon occurs in Parkinson's disease (PD) with the build-up of Lewy bodies. The analysis of senile plaques, and other brain samples, from AD and PD patients by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has advantages but also presents obstacles due to the nature of the processes utilized in isolation procedures and storage. Salts, buffers, and detergents necessary in isolation of biological species may cause adducts and ion suppression that convolute the spectra obtained...
November 7, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29110928/direct-bacterial-identification-from-positive-blood-cultures-using-matrix-assisted-laser-desorption-ionization-time-of-flight-maldi-tof-mass-spectrometry-a-systematic-review-and-meta-analysis
#17
Jesús Ruiz-Aragón, Mónica Ballestero-Téllez, Belén Gutiérrez-Gutiérrez, Marina de Cueto, Jesús Rodríguez-Baño, Álvaro Pascual
INTRODUCTION: The rapid identification of bacteraemia-causing pathogens could assist clinicians in the timely prescription of targeted therapy, thereby reducing the morbidity and mortality of this infection. In recent years, numerous techniques that rapidly and directly identify positive blood cultures have been marketed, with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) being one of the most commonly used. METHODS: The aim of this systematic review and meta-analysis was to evaluate the accuracy of MALDI-TOF (Bruker(®)) for the direct identification of positive blood culture bottles...
October 27, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29108681/legionella-contamination-in-warm-water-systems-a-species-level-survey
#18
Thorsten Dilger, Holger Melzl, André Gessner
Legionellae constitute a frequent contamination of warm water systems and can lead to serious infections. Therefore, in many countries it is mandatory to monitor warm water systems for their presence. The method of examination in Germany is regulated by guideline ISO 11731 and DIN EN ISO 11731-2, and the results are reported as concentration of Legionella spp. Only limited information is available on the presence of individual species of Legionellae in the examined systems, since most investigations and research focus solely on Legionella pneumophila as the most important human pathogen...
November 3, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29105868/successful-treatment-of-disseminated-nocardiosis-caused-by-nocardia-veterana-in-a-dog
#19
S Yaemsiri, J E Sykes
A 5-year-old male castrated Lhasa Apso cross was evaluated for a 1-month history of inappetence, lethargy, gagging, and progressive right thoracic limb lameness. Synovial fluid analysis revealed nonseptic suppurative inflammation, and a diagnosis of immune-mediated polyarthritis (IMPA) was made. After 3 months of treatment with prednisone and later cyclosporine, the dog developed multiple firm cutaneous and subcutaneous masses and a focal mass within the jejunum. Cultures of blood, urine, skin lesions, and the jejunal mass identified Nocardia veterana by matrix-absorption laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and allowed for earlier identification of the organism compared to more traditional secA1 gene sequencing...
November 4, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29105571/accuracy-of-pcr-targeting-different-markers-for-staphylococcus-aureus-identification-a-comparative-study-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-as-the-gold-standard
#20
Mauro M Saraiva, Candice M De Leon, Silvana C Santos, Danilo T Stipp, Miliane M Souza, Lauro Santos Filho, Wondwossen A Gebreyes, Celso J Oliveira
Staphylococcus aureus is considered a major pathogen in veterinary and human medicine, and the emergence of multidrug-resistant strains, such as livestock-associated methicillin-resistant S. aureus, means that reliable, inexpensive, and fast methods are required to identify S. aureus obtained from animal sources. We tested the accuracy of a PCR targeting the genes femA, nuc, and coa in identifying S. aureus from animals. A total of 157 Staphylococcus spp. isolates were examined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry; 18 different Staphylococcus species were identified...
November 1, 2017: Journal of Veterinary Diagnostic Investigation
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