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https://www.readbyqxmd.com/read/28813780/microbial-diversity-of-commercial-makgeolli-and-its-influence-on-the-organoleptic-characteristics-of-korean-rice-sourdough-jeung-pyun
#1
Jaehyung Park, Ji Sun Seo, Seul-Ah Kim, So-Yeon Shin, Jong-Hyun Park, Nam Soo Han
Sourdough is made by fermentation of dough by lactic acid bacteria (LAB) and yeast to improve bread properties like volume, flavor, and texture. A Korean traditional sourdough was made by fermenting rice flour with rice wine (makgeolli) and used to make sponge-like bread (Jeung-pyun). The aim of this study was to investigate the microbial diversity of makgeolli products and their influence on the organoleptic quality of Jeung-pyun. Three commercial makgeolli were tested for Jeung-pyun production, with each product exhibiting varied dough swelling rates and organoleptic qualities, and among them, J-product was ranked highest in texture and taste...
August 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28813445/transition-of-the-intestinal-microbiota-of-cats-with-age
#2
Hiroaki Masuoka, Kouya Shimada, Tomoyo Kiyosue-Yasuda, Masaharu Kiyosue, Yukie Oishi, Seiji Kimura, Yuji Ohashi, Tomohiko Fujisawa, Kozue Hotta, Akio Yamada, Kazuhiro Hirayama
The transition of intestinal microbiota with age has been well described in humans. However, the age-related changes in intestinal microbiota of cats have not been well studied. In the present study, we investigated the composition of intestinal microbiota of cats in 5 different age groups (pre-weanling, weanling, young, aged, senile) with a culture-based method. For lactobacilli and bifidobacteria, we also quantified with molecular-based method, real-time PCR. The results suggested that the composition of the feline intestinal microbiota changes with age, while the changes were different from those of humans and dogs...
2017: PloS One
https://www.readbyqxmd.com/read/28811215/molecular-mechanisms-underlying-protection-against-h9n2-influenza-virus-challenge-in-mice-by-recombinant-lactobacillus-plantarum-with-surface-displayed-ha2-ltb
#3
Yanlong Jiang, Guilian Yang, Qi Wang, Zhannan Wang, Wentao Yang, Wei Gu, Chunwei Shi, Jianzhong Wang, Haibin Huang, Chunfeng Wang
It has been considered that the Avian influenza virus (AIV) causes severe threats to poultry industry. In this study, we constructed a series of recombinant Lactobacillus plantarum (L. plantarum) with surface displayed hemagglutinin subunit 2 (HA2) alone or together with heat-labile toxin B subunit (LTB) from enterotoxigenic Escherichia coli. Balb/c mice were used as model to evaluate the protective effects of recombinant L. plantarum strains against H9N2 subtype challenge. The results showed that the presence of LTB significantly increased the percentages of CD3(+)CD4(+)IL-4(+), CD3(+)CD4(+)IFN-γ(+) and CD3(+)CD4(+)IL-17(+) T cells, as well as CD3(+)CD8(+)IFN-γ(+) T cells in spleen and MLNs determined by Fluorescence-Activated Cell Sorting assay...
August 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28808024/structural-and-functional-studies-of-pyruvate-carboxylase-regulation-by-cyclic-di-amp-in-lactic-acid-bacteria
#4
Philip H Choi, Thu Minh Ngoc Vu, Huong Thi Pham, Joshua J Woodward, Mark S Turner, Liang Tong
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC), a biotin-dependent enzyme with biotin carboxylase (BC) and carboxyltransferase (CT) activities. We report here structural, biochemical, and functional studies on the inhibition of Lactococcus lactis PC (LlPC) by c-di-AMP...
August 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28802013/effects-of-levan-type-fructan-on-growth-performance-nutrient-digestibility-diarrhea-scores-fecal-shedding-of-total-lactic-acid-bacteria-and-coliform-bacteria-and-fecal-gas-emission-in-weaning-pigs
#5
Xin Jian Lei, Yong Min Kim, Jae Hong Park, Dong Heon Baek, Charles Martin Nyachoti, In Ho Kim
BACKGROUND: The use of antibiotics as growth promoters in feed has been fully or partially banned in several countries. The objective of this study was to evaluate effects of levan-type fructan on growth performance, nutrient digestibility, fecal shedding of lactic acid bacteria and coliform bacteria, diarrhea scores, and fecal gas emission in weaning pigs. A total of 144 weaning pigs [(Yorkshire × Landrace) × Duroc] were randomly allocated to 4 diets: corn-soybean meal-based diets supplemented with 0, 0...
August 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28802003/recombinant-lactococcus-lactis-for-efficient-conversion-of-cellodextrins-into-l-lactic-acid
#6
Chiara Gandini, Loredana Tarraran, Denis Kalemasi, Enrica Pessione, Roberto Mazzoli
Lactic acid bacteria (LAB) are among the most interesting organisms for industrial processes with a long history of application as food starters and biocontrol agents, and an underexploited potential for biorefineries converting biomass into high-value compounds. Lactic acid (LA), their main fermentation product, is among the most requested chemicals owing to its broad range of applications. Notably, LA polymers, i.e., polylactides, have high potential as biodegradable substitutes of fossil-derived plastics...
August 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28800820/bacterial-diversity-of-the-colombian-fermented-milk-suero-coste%C3%A3-o-assessed-by-culturing-and-high-throughput-sequencing-and-dgge-analysis-of-16s-rrna-gene-amplicons
#7
Karina Edith Motato, Christian Milani, Marco Ventura, Francia Elena Valencia, Patricia Ruas-Madiedo, Susana Delgado
"Suero Costeño" (SC) is a traditional soured cream elaborated from raw milk in the Northern-Caribbean coast of Colombia. The natural microbiota that characterizes this popular Colombian fermented milk is unknown, although several culturing studies have previously been attempted. In this work, the microbiota associated with SC from three manufacturers in two regions, "Planeta Rica" (Córdoba) and "Caucasia" (Antioquia), was analysed by means of culturing methods in combination with high-throughput sequencing and DGGE analysis of 16S rRNA gene amplicons...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28799082/mathematical-model-of-interaction-between-bacteriocin-producing-lactic-acid-bacteria-and-listeria-part-2-bifurcations-and-applications
#8
Roberta Regina Delboni, Hyun Mo Yang
The big challenge for the food industry is the attending to demands for minimally processed foods, avoiding intense heat treatments and reducing the addition of chemical preservatives, but at the same time ensuring microbiological safety of these products. Lactic acid bacteria are traditionally used in the production of fermented foods. They are responsible for the production of antimicrobial compounds, such as organic acids and bacteriocins, which are protein compounds with bactericidal effect against related species and bacteria such as Listeria monocytogenes and Staphylococcus aureus...
August 10, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28792667/highly-enantioselective-production-of-chiral-secondary-alcohols-using-lactobacillus-paracasei-bd101-as-a-new-whole-cell-biocatalyst-and-evaluation-of-their-antimicrobial-effects
#9
Durmuşhan Yılmaz, Engin Şahin, Enes Dertli
Chiral secondary alcohols are valuable intermediates for many important enantiopure pharmaceuticals and biologically active molecules. In this work, we studied asymmetric reduction of aromatic ketones to produce the corresponding chiral secondary alcohols using lactic acid bacteria (LAB) as new biocatalysts. Seven LAB strains were screened for their ability to reduce acetophenones to their corresponding alcohols. Among these strains Lactobacillus paracasei BD101, was found to be the most successful at reducing the ketones to the corresponding alcohols...
August 9, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28791000/starch-flocculation-by-the-sweet-potato-sour-liquid-is-mediated-by-the-adhesion-of-lactic-acid-bacteria-to-starch
#10
Lili Zhang, Yang Yu, Xinhua Li, Xiaona Li, Huajiang Zhang, Zhen Zhang, Yunhe Xu
In the current study, we focused on the mechanism underlying starch flocculation by the sweet potato sour liquid. The traditional microbial techniques and 16S rDNA sequencing revealed that Lactobacillus was dominant flocculating microorganism in sour liquid. In total, 86 bacteria, 20 yeasts, and 10 molds were isolated from the sour liquid and only eight Lactobacillus species exhibited flocculating activity. Lactobacillus paracasei subsp. paracasei L1 strain with a high flocculating activity was isolated and identified, and the mechanism of starch flocculation was examined...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28783535/the-individual-contribution-of-starter-and-non-starter-lactic-acid-bacteria-to-the-volatile-organic-compound-composition-of-caciocavallo-palermitano-cheese
#11
Valeria Guarrasi, Ciro Sannino, Marta Moschetti, Adriana Bonanno, Antonino Di Grigoli, Luca Settanni
The contribution of two starter (Lactobacillus delbrueckii and Streptococcus thermophilus) and nine non-starter (Enterococcus casselliflavus, Enterococcus faecalis, Enterococcus durans, Enterococcus gallinarum, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Pediococcus acidilactici and Pediococcus pentosaceus) species of lactic acid bacteria (LAB) to the volatile organic compounds (VOCs) of Caciocavallo Palermitano cheese was investigated. The strains used in this study were isolated during the production/ripening of the stretched cheese and tested in a cheese-based medium (CBM)...
July 31, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28783248/coupling-metagenomics-with-cultivation-to-select-host-specific-probiotic-microorganisms-for-subtropical-aquaculture
#12
Jinming Cui, Minfeng Xiao, Mingzhu Liu, Zuowei Wang, Furong Liu, Liang Guo, Hailin Meng, Honggang Zhang, Jinfang Yang, Deng Deng, Shuangqiang Huang, Yingfei Ma, Chenli Liu
AIMS: To demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry. METHODS AND RESULTS: By using both culture-dependent and -independent methods, we have systematically investigated, for the first time, the gut microbiota of twelve subtropical aquatic animal species. We found that the diversity, abundance, and distribution of gut microorganisms of these animals were host-specific, and that lactic acid bacteria (LAB) were predominant among the indigenous probiotic microbes...
August 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28783048/exploring-the-behavior-and-metabolic-transformations-of-senps-in-exposed-lactic-acid-bacteria-effect-of-nanoparticles-coating-agent
#13
Maria Palomo-Siguero, Yolanda Madrid
The behavior and transformation of selenium nanoparticles (SeNPs) in living systems such as microorganisms is largely unknown. To address this knowledge gap, we examined the effect of three types of SeNP suspensions toward Lactobacillus delbrueckii subsp. bulgaricus LB-12 using a variety of techniques. SeNPs were synthesized using three types of coating agents (chitosan (CS-SeNPs), hydroxyethyl cellulose (HEC-SeNPs) and a non-ionic surfactant, surfynol (ethoxylated-SeNPs)). Morphologies of SeNPs were all spherical...
August 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28780720/evaluation-of-lactic-acid-bacteria-isolated-from-fermented-plant-products-for-antagonistic-activity-against-urinary-tract-pathogen-staphylococcus-saprophyticus
#14
Cheng-Chih Tsai, Tzu-Min Lai, Pei-Pei Lin, You-Miin Hsieh
Urinary tract infections (UTIs) are the most common infectious diseases in infants and the elderly; they are also the most common among nosocomial infections. The treatment of UTIs usually involves a short-term course of antibiotics. The purpose of this study was to identify the strains of lactic acid bacteria (LAB) that can inhibit the urinary tract pathogen Staphylococcus saprophyticus, as alternatives to antibiotics. In this study, we collected 370 LAB strains from fermented plant products and reference strains from the Bioresources Collection and Research Center (BCRC)...
August 5, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28780605/subcellular-membrane-fluidity-of-lactobacillus-delbrueckii-subsp-bulgaricus-under-cold-and-osmotic-stress
#15
Julie Meneghel, Stéphanie Passot, Stéphanie Cenard, Matthieu Réfrégiers, Frédéric Jamme, Fernanda Fonseca
Cryopreservation of lactic acid bacteria may lead to undesirable cell death and functionality losses. The membrane is the first target for cell injury and plays a key role in bacterial cryotolerance. This work aimed at investigating at a subcellular resolution the membrane fluidity of two populations of Lactobacillus delbrueckii subsp. bulgaricus when subjected to cold and osmotic stresses associated to freezing. Cells were cultivated at 42 °C in mild whey medium, and they were exposed to sucrose solutions of different osmolarities (300 and 1800 mOsm L(-1)) after harvest...
August 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28774859/conjugal-transfer-of-aac-6-ie-aph-2%C3%A2-ia-gene-from-native-species-and-mechanism-of-regulation-and-cross-resistance-in-enterococcus-faecalis-mcc3063-by-real-time-pcr
#16
G Jaimee, P M Halami
High level aminoglycoside resistance (HLAR) in the lactic acid bacteria (LAB) derived from food animals is detrimental. The aim of this study was to investigate the localization and conjugal transfer of aminoglycoside resistance genes, aac(6')Ie-aph(2″)Ia and aph(3')IIIa in different Enterococcus species. The cross resistance patterns in Enterococcus faecalis MCC3063 to clinically important aminoglycosides by real time PCR were also studied. Southern hybridization experiments revealed the presence of aac(6')Ie-aph(2(″))Ia and aph(3')IIIa genes conferring HLAR in high molecular weight plasmids except in Lactobacillus plantarum...
July 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28772193/effects-of-chilled-then-frozen-storage-up-to-52weeks-on-lamb-m-longissimus-lumborum-quality-and-safety-parameters
#17
Cassius E O Coombs, Benjamin W B Holman, Damian Collins, Michael A Friend, David L Hopkins
This study evaluated the effect of chilled followed by frozen storage on lamb quality and safety parameters. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24 h post-mortem, and assigned to five chilled storage periods (0, 2, 4, 6 and 8 weeks) and six subsequent frozen storage periods (0, 4, 8, 12, 24 and 52 weeks). Upon completion of each storage treatment combination, corresponding LL were sub-sectioned and analysed for colour stability (0, 1, 2 and 3 days), shear force, fluid losses (purge, thaw and cooking losses), intramuscular fat content, sarcomere length, water activity and microbial load (lactic acid bacteria, Enterobacteriaceae sp...
July 26, 2017: Meat Science
https://www.readbyqxmd.com/read/28770479/effect-of-milk-fermented-with-lactic-acid-bacteria-on-diarrheal-incidence-growth-performance-and-microbiological-and-blood-profiles-of-newborn-dairy-calves
#18
N C Maldonado, J Chiaraviglio, E Bru, L De Chazal, V Santos, M E F Nader-Macías
The effect of the administration of milk fermented with lactic acid bacteria to calves was evaluated. The strains included were: Lactobacillus murinus CRL1695, Lact. mucosae CRL1696, Lact. johnsonii CRL1693, and Lact. salivarius CRL1702, which were selected for their beneficial and functional properties and isolated from healthy calves in the northwestern region of Argentina. The trial was conducted on a dairy farm located in Tucumán (Holando-Argentino calves). A randomized controlled trial was performed in which 56 new-born animals were divided into two groups: the treated group (T) received the fermented milk for 60 days and the control group (C) only milk...
August 2, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28769917/identification-and-functional-validation-of-autolysis-associated-genes-in-lactobacillus-bulgaricus-atcc-baa-365
#19
Xiaoyang Pang, Shuwen Zhang, Jing Lu, Lu Liu, Changlu Ma, Yang Yang, Panpan Ti, Weihua Gao, Jiaping Lv
Lactic acid bacteria (LAB) are important organisms in food production. Indeed, LAB autolysis is very critical in dairy processing. For example, it influences the development of cheese flavor by releasing intracellular enzymes, and controls cell growth in yogurts and probiotic products. Two component systems (TCS) constitute essential environmental sensors and effectors of signal transduction in most bacteria. In the present work, mutants of one TCS (LBUL_RS00115/LBUL_RS00110) were generated to assess the relationship between TCS and cell autolysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28769914/isolation-characterization-and-selection-of-molds-associated-to-fermented-black-table-olives
#20
Simona L Bavaro, Antonia Susca, Jens C Frisvad, Maria Tufariello, Agathi Chytiri, Giancarlo Perrone, Giovanni Mita, Antonio F Logrieco, Gianluca Bleve
Table olives are one of the most important fermented food in the Mediterranean countries. Apart from lactic acid bacteria and yeasts that mainly conduct the olive fermentation, molds can develop on the brine surface, and can have either deleterious or useful effects on this process. From the food safety point of view, occurring molds could also produce mycotoxins, so, it is important to monitor and control them. In this respect, identification of molds associated to two Italian and two Greek fermented black table olives cultivars, was carried out...
2017: Frontiers in Microbiology
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