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https://www.readbyqxmd.com/read/28937854/probiotics-from-isolation-to-application
#1
Parisa Shokryazdan, Mohammad Faseleh Jahromi, Juan Boo Liang, Yin Wan Ho
Probiotics have become highly recognized as supplements for humans and animals because of their beneficial effects on health and well-being. The present review aims to provide an overview of different steps through which microbial strains become applicable probiotics in food and/or feed industries. Isolation of potential probiotic strains is the first step. Lactic acid bacteria are the most frequently used microorganisms as probiotics, which can be isolated from human, animal, plant, and environment. The next steps are identification of the isolates and characterization of them based on the main selection criteria for any potential probiotic microorganism, including resistance to gastric acidity and bile salt, adherence to mucus and/or intestinal epithelial cells and cell lines, and antimicrobial and antagonism activity against potentially pathogenic microbes...
September 22, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28936203/modulation-of-gut-microbiome-composition-and-function-in-experimental-colitis-treated-with-sulfasalazine
#2
Haihui Zheng, Mingyi Chen, Yuan Li, Yuanyuan Wang, Lin Wei, Ziqiong Liao, Mengxia Wang, Fangli Ma, Qiongfeng Liao, Zhiyong Xie
Inflammatory bowel disease (IBD) results from alterations in intestinal flora and the immune system. Sulfasalazine (SASP) is a sulfa antimicrobial used to treat IBD in clinic for years. However, how SASP affects gut microbes and its potential functions remains unclear. To investigate the relationships of SASP, IBD, and gut microbiome, we used 2,4,6-trinitrobenzenesulfonic acid (TNBS) to induce experimental colitis in rats, and analyzed the microbiota in the fecal samples, which come from the control group (treated with ethanol + saline), the model group (treated with TNBS-ethanol + saline) and the SASP group (treated with TNBS-ethanol + SASP), with 16S gene sequencing and followed up a subset sample using shotgun sequencing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28935539/molecular-structure-of-cyclomaltodextrinase-derived-from-amylolytic-lactic-acid-bacterium-enterococcus-faecium-k-1-and-properties-of-recombinant-enzymes-expressed-in-escherichia-coli-and-lactobacillus-plantarum
#3
Kridsada Unban, Apinun Kanpiengjai, Saisamorn Lumyong, Thu-Ha Nguyen, Dietmar Haltrich, Chartchai Khanongnuch
Gene encoding cyclomaltodextrinase (Cdx) from amylolytic lactic acid bacterium Enterococcus faecium K-1 was cloned and nucleotide sequence was analyzed. The open-reading frame consisted of 1767bp encoding 588 deduced amino acids. Consequently, four typically conserved regions of the glycoside hydrolase family 13 were revealed; however, nine exceeding amino acids (DSYQMTDVP) were found at the 282-290 position in comparison to previously reported cyclomaltodextrinases. This difference is believed to have an influence on the substrate specificity of this enzyme...
September 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28933759/lactic-acid-bacteria-and-their-bacteriocins-classification-biosynthesis-and-applications-against-uropathogens-a-mini-review
#4
REVIEW
Mduduzi Paul Mokoena
Several lactic acid bacteria (LAB) isolates from the Lactobacillus genera have been applied in food preservation, partly due to their antimicrobial properties. Their application in the control of human pathogens holds promise provided appropriate strains are scientifically chosen and a suitable mode of delivery is utilized. Urinary tract infection (UTI) is a global problem, affecting mainly diabetic patients and women. Many uropathogens are developing resistance to commonly used antibiotics. There is a need for more research on the ability of LAB to inhibit uropathogens, with a view to apply them in clinical settings, while adhering to strict selection guidelines in the choice of candidate LAB...
July 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28929049/antifungal-effects-of-lactobacillus-species-isolated-from-local-dairy-products
#5
Sahar Karami, Mohammad Roayaei, Elnaz Zahedi, Mahmoud Bahmani, Leila Mahmoodnia, Hosna Hamzavi, Mahmoud Rafieian-Kopaei
OBJECTIVE: The Lactobacillus is a genus of lactic acid bacteria which are regularly rod-shaped, nonspore, Gram-positive, heterogeneous, and are found in a wide range of inhabitants such as dairy products, plants, and gastrointestinal tract. A variety of antimicrobial compounds and molecules such as bacteriocin are produced by these useful bacteria to inhibit the growth of pathogenic microbes in the food products. This paper aims to examine the isolation of Lactobacillus from local dairies as well as to determine their inhibition effect against a number of pathogens, such as two fungi: Penicillium notatum and Aspergillus fulvous...
April 2017: International Journal of Pharmaceutical Investigation
https://www.readbyqxmd.com/read/28928534/suitability-of-the-probiotic-lactic-acid-bacteria-strains-as-the-starter-cultures-in-unripe-cornelian-cherry-cornus-mas-l-fermentation
#6
Agata Czyżowska, Alicja Z Kucharska, Agnieszka Nowak, Anna Sokół-Łętowska, Ilona Motyl, Narcyz Piórecki
This study examines the influence of lactic acid fermentation on iridoid and polyphenolic compounds in unripe Cornus mas L. fruits. Fermentation was carried out at room temperature over 6 weeks. Eight versions of the fermentation process were conducted: with inoculation by microflora isolated from black olives, Lb. brevis 18 M, Lb. casei 0919, Lb. paracasei 920, Lb. rhamnosus 0900, Lb. rhamnosus 0908, and spontaneous fermentation with and without summer savory (Satureja hortensis). The polyphenolic compounds, iridoids and antioxidant activities were examined in the products obtained...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28928502/accelerated-fermentation-of-idli-batter-using-eleusine-coracana-and-pennisetum-glaucum
#7
Ramachandran Chelliah, Sudha Rani Ramakrishnan, Divya Premkumar, Usha Antony
The present study focuses on reduction in fermentation time of idli batter and its enrichment with calcium and iron using finger millet and pearl millet. Fermentation time decreased from 12 to 6 h with addition of finger millet and to 8 h with pearl millet. Rate of fermentation, bulk density, viscosity, microbial changes and particle morphology were assessed in the batter; nutritional quality, sensory, phytochemicals and internal characteristics of idli were investigated. The color and texture characteristics of both batter and idli were evaluated...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28927834/microbial-behavior-and-changes-in-food-constituents-during-fermentation-of-japanese-sourdoughs-with-different-rye-and-wheat-starting-materials
#8
Akihito Fujimoto, Keisuke Ito, Madoka Itou, Noriko Narushima, Takayuki Ito, Akihisa Yamamoto, Satoru Hirayama, Soichi Furukawa, Yasushi Morinaga, Takahisa Miyamoto
Sourdough is a food item made by kneading grain flour and water together and allowing fermentation through the action of lactic acid bacteria (Lactobacillales) and yeast. Typically, Japanese bakeries make sourdough with rye flour, wheat flour, malt extract, and water and allow spontaneous fermentation for 6 days. We compared the microbial behavior and food components, such as organic acids, sugars, and free amino acids, of sourdoughs made using two different rye and wheat flours during the 6-day fermentation period...
September 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28921480/the-biodiversity-of-lactobacillus-spp-from-iranian-raw-milk-motal-cheese-and-antibacterial-evaluation-based-on-bacteriocin-encoding-genes
#9
Fahimeh Azizi, Mohammad B Habibi Najafi, Mohammad R Edalatian Dovom
Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify the Lactobacillus spp. isolated from Iranian raw milk Motal cheese and to detect the presence of bacteriocin genes in the isolated Lactobacillus strains exhibiting antimicrobial activity. For this purpose, 6 Motal cheese samples from Dasht-e-Moghan region, Iran, were subjected to microbial characterization...
September 18, 2017: AMB Express
https://www.readbyqxmd.com/read/28921417/bacteriocinogenic-lab-strains-for-fermented-meat-preservation-perspectives-challenges-and-limitations
#10
Lorenzo Favaro, Svetoslav Dimitrov Todorov
Over the last decades, much research has focused on lactic acid bacteria (LAB) bacteriocins because of their potential as biopreservatives and their action against the growth of spoilage microbes. Meat and fermented meat products are prone to microbial contamination, causing health risks, as well as economic losses in the meat industry. The use of bacteriocin-producing LAB starter or protective cultures is suitable for fermented meats. However, although bacteriocins can be produced during meat processing, their levels are usually much lower than those achieved during in vitro fermentations under optimal environmental conditions...
September 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28918154/hot-topic-geographical-distribution-and-strain-diversity-of-lactobacillus-wasatchensis-isolated-from-cheese-with-unwanted-gas-formation
#11
Michele Culumber, Donald J McMahon, Fatih Ortakci, Lauren Montierth, Beatriz Villalba, Jeffery R Broadbent, Craig J Oberg
Lactobacillus wasatchensis, an obligate heterofermentative nonstarter lactic acid bacteria (NSLAB) implicated in causing gas defects in aged cheeses, was originally isolated from an aged Cheddar produced in Logan, Utah. To determine the geographical distribution of this organism, we isolated slow-growing NSLAB from cheeses collected in different regions of the United States, Australia, New Zealand, and Ireland. Seven of the cheeses showed significant gas defects and 12 did not. Nonstarter lactic acid bacteria were isolated from these cheeses on de Man, Rogosa, and Sharpe medium supplemented with ribose, a preferred substrate for Lb...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28918152/short-communication-influence-of-various-proteolytic-sources-during-fermentation-of-reconstituted-corn-grain-silages
#12
D Junges, G Morais, M H F Spoto, P S Santos, A T Adesogan, L G Nussio, J L P Daniel
The objective of this study was to determine the contribution of corn kernel enzymes, bacteria, fungi, and fermentation end-products (main acids and ethanol) to protein solubilization during fermentation of reconstituted corn grain silage. Flint corn kernels were ground (5-mm sieve), rehydrated to 32% of moisture, and treated with no additives (control), gamma irradiation (32 kGy), gamma irradiation + fermentation end-products (1% of lactic acid, 0.3% of acetic acid, and 0.7% of ethanol, as fed), and natamycin (1% as fed)...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28911652/microbiological-histological-and-biochemical-evidence-for-the-adverse-effects-of-food-azo-dyes-on-rats
#13
Khaled Elbanna, Osama Mohamed Sarhan, Manal Khider, Mohamed Elmogy, Hussein Hasan Abulreesh, Mohamed Rabie Shaaban
In this study, 120 lactic acid bacterial strains from different fermented dairy products as well as 10 bacterial intestinal isolates were evaluated for in vitro and in vivo degradation of various food azo dyes. Of these isolates, lactic acid bacteria (LAB) strains 13 and 100 and the intestinal isolates Ent2 and Eco5 exhibited 96-98% degradation of the tested food azo dyes within 5-6 hours. High performance liquid chromatography mass spectra of sunset yellow (E110) and carmoisine (E122) anaerobic degradation products by the intestinal isolates showed that they were structurally related to toxic aromatic amines...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910550/overview-of-craft-brewing-specificities-and-potentially-associated-microbiota
#14
Lindsey Rodhouse, Franck Carbonero
The brewing process differs slightly in craft breweries as compared to industrial breweries, as there are fewer control points. This arguably affects the microbiota of the final product. Beer contains several antimicrobial properties that protect it from pathogens, such as low pH, low oxygen and high carbon dioxide content, and the addition of hops. However, these hurdles have limited power controlling spoilage organisms. Contamination by these organisms can originate in the raw materials, persist in the environment, and be introduced by using flavoring ingredients later in the process...
September 14, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28910141/rapid-screening-of-natural-plant-extracts-with-calcium-diacetate-for-differential-effects-against-foodborne-pathogens-and-a-probiotic-bacterium
#15
William Colonna, Byron Brehm-Stecher, Kalidas Shetty, Anthony Pometto Iii
This study focused on advancing a rapid turbidimetric bioassay to screen antimicrobials using specific cocktails of targeted foodborne bacterial pathogens. Specifically, to show the relevance of this rapid screening tool, the antimicrobial potential of generally recognized as safe calcium diacetate (DAX) and blends with cranberry (NC) and oregano (OX) natural extracts was evaluated. Furthermore, the same extracts were evaluated against beneficial lactic acid bacteria. The targeted foodborne pathogens evaluated were Escherichia coli O157:H7, Salmonella spp...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28906433/effect-of-environmental-factors-on-intra-specific-inhibitory-activity-of-carnobacterium-maltaromaticum
#16
Peipei Zhang, Mandeep Kaur, John P Bowman, David A Ratkowsky, Mark Tamplin
Carnobacterium maltaromaticum is frequently associated with foods having extended shelf-life due to its inhibitory activity to other bacteria. The quantification of such inhibition interactions affected by various environmental factors is limited. This study investigated the effect of environmental factors relevant to vacuum-packaged beef on inhibition between two model isolates of C. maltaromaticum, D0h and D8c, specifically D8c sensitivity to D0h inhibition and D0h inhibitor production. The effects of temperature (-1, 7, 15, 25 °C), atmosphere (aerobic and anaerobic), pH (5...
September 14, 2017: Microorganisms
https://www.readbyqxmd.com/read/28905506/nutritive-value-and-fermentation-quality-of-palisadegrass-and-stylo-mixed-silages
#17
Juliana S da Silva, Karina G Ribeiro, Odilon G Pereira, Hilário C Mantovani, Paulo R Cecon, Rosana C Pereira, Janaina de L Silva
The nutritive value and fermentation quality of palisadegrass (Brachiaria brizantha cv. Xaraes) and stylo (Stylosanthes capitata × S. macrocephala cv. Campo Grande) mixed silages were evaluated. The experiment was analyzed in a factorial scheme (5 × 2) in a completely randomized design using increasing levels of stylo (0, 25, 50, 75 and 100% on a fresh matter basis) on palisadegrass silages, with and without microbial inoculants (MI). With the increased ratio of stylo in mixed silages, dry matter (DM), crude protein (CP), acid detergent fiber (ADF), and lignin content increased in silages...
September 14, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28905285/effect-of-bacteriocin-and-exopolysaccharides-isolated-from-probiotic-on-p-aeruginosa-pao1-biofilm
#18
Vivek Sharma, Kusum Harjai, Geeta Shukla
Microorganisms develop biofilms on indwelling medical devices and are associated with biofilm-related infections, resulting in substantial morbidity and mortality. Therefore, to prevent and control biofilm-associated infections, the present study was designed to assess the anti-biofilm potential of postbiotics derived from probiotic organisms against most prevalent biofilm-forming Pseudomonas aeruginosa PAO1. Eighty lactic acid bacteria isolated from eight neonatal fecal samples possessed antibacterial activity against P...
September 14, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28905106/recombinant-mouse-osteocalcin-secreted-by-lactococcus-lactis-promotes-glucagon-like-peptide-1-induction-in-stc-1-cells
#19
Fu Namai, Suguru Shigemori, Koichi Sudo, Takashi Sato, Yoshinari Yamamoto, Shireen Nigar, Tasuku Ogita, Takeshi Shimosato
An osteoblastic protein, osteocalcin (OC), exists in vivo in two forms: carboxylated OC, and uncarboxylated or low-carboxylated OC (ucOC). ucOC acts as a hormone to regulate carbon and energy metabolism. Recent studies demonstrated that ucOC exerts insulinotropic effects, mainly through the glucagon-like peptide 1 (GLP-1) pathway. GLP-1 is an insulinotropic hormone secreted by enteroendocrine L cells in the small intestine. Thus, efficient delivery of ucOC to the small intestine may be a new therapeutic option for metabolic diseases such as diabetes and obesity...
September 13, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28905008/probiotics-as-a-preventive-strategy-for-surgical-infection-in-colorectal-cancer-patients-a-systematic-review-and-meta-analysis-of-randomized-trials
#20
Priscilla Régia de Andrade Calaça, Raquel Pedrosa Bezerra, Wendell Wagner Campos Albuquerque, Ana Lúcia Figueiredo Porto, Maria Taciana Holanda Cavalcanti
BACKGROUND: Infection following abdominal surgery remains a major factor in morbidity among colorectal cancer (CRC) patients. Probiotic therapy has been suggested to improve the clinical and laboratory outcome of patients undergoing gastrointestinal surgery. The aim of this study was to investigate the efficacy of probiotic lactic acid bacteria in patients with CRC in the pre- and postoperative phases. METHODS: Systematic database searches identified 1,080 related articles...
2017: Translational Gastroenterology and Hepatology
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