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https://www.readbyqxmd.com/read/29895334/ex-vivo-evaluation-of-intravitreal-mesenchymal-stromal-cell-viability-using-bioluminescence-imaging
#1
Carolina Assis P Vilela, Lucas Eduardo B Souza, Rubens C Siqueira, Rodrigo T Calado, Dimas T Covas, Jayter S Paula
BACKGROUND: Bone marrow-derived mesenchymal stromal cell (MSC) therapy is a promising treatment for several degenerative ocular diseases; however, no reproducible method of monitoring these cells into the eye has been established. The aim of this study was to describe successful bioluminescence imaging (BLI) to detect viable luciferase-expressing MSC in the eye. METHODS: Human donor MSC in culture were transduced with 50 μl luciferase lentiviral vector (three viral particles/cell) prior to intraocular injection...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29879524/course-of-serological-tests-in-treated-subjects-with-chronic-trypanosoma-cruzi-infection-a-systematic-review-and-meta-analysis-of-individual-participant-data
#2
REVIEW
Yanina Sguassero, Karen N Roberts, Guillermina B Harvey, Daniel Comandé, Agustín Ciapponi, Cristina B Cuesta, Emmaría Danesi, Camila Aguiar, Ana L Andrade, Ana Mde Castro, Marta de Lana, Josep M Escribà, Diana L Fabbro, Cloé D Fernandes, Wendell Sf Meira, María Flores-Chávez, Alejandro M Hasslocher-Moreno, Yves Jackson, Carlos D Lacunza, Girley F Machado-de-Assis, Marisel Maldonado, María M Monje-Rumi, Israel Molina, Catalina Muñoz-San Martín, Laura Murcia, Cleudson Nery de Castro, Celeste An Silveira, Olga Sánchez Negrette, Manuel Segovia, Aldo Solari, Mário Steindel, Mirtha L Streiger, Ninfa Vera de Bilbao, Inés Zulantay, Sergio Sosa-Estani
OBJECTIVE: To determine the course of serological tests in subjects with chronic T. cruzi infection treated with antitrypanosomal drugs. METHODS: We conducted a systematic review and meta-analysis using individual participant data. Survival analysis and Cox proportional hazards regression model with a random effect to adjust for covariates were applied. The protocol was registered at www.crd.york.ac.uk/PROSPERO (CRD42012002162). RESULTS: We included 27 studies (1296 subjects) conducted in eight countries...
June 4, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29797194/erythrocyte-acetylcholinesterase-as-biomarker-of-pesticide-exposure-new-and-forgotten-insights
#3
Caio R D Assis, Amanda G Linhares, Mariana P Cabrera, Vagne M Oliveira, Kaline C C Silva, Marina Marcuschi, Elba V M Maciel Carvalho, Ranilson S Bezerra, Luiz B Carvalho
Acetylcholinesterase (AChE) acts on the hydrolysis of acetylcholine, rapidly removing this neurotransmitter at cholinergic synapses and neuromuscular junctions as well as in neuronal growth and differentiation, modulation of cell adhesion ("electrotactins") and aryl-acylamidase activity (AAA). This enzyme is also found in erythrocyte, as 160 kDa dimer that anchors to the plasma membrane via glycophosphatidylinositol. The function of this enzyme in erythrocytes has not yet been elucidated; however, it is suspected to participate in cell-to-cell interactions...
May 24, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29780820/coadministration-of-nematophagous-fungi-for-biological-control-over-nematodes-in-bovine-in-the-south-eastern-brazil
#4
Fábio Dias Luns, Rafaela Carolina Lopes Assis, Laryssa Pinheiro Costa Silva, Carolina Magri Ferraz, Fábio Ribeiro Braga, Jackson Victor de Araújo
This study compared the coadministration among the three nematode predatory fungi, Duddingtonia flagrans , Monacrosporium thaumasium , and Arthrobotrys robusta , in the biological control of cattle gastrointestinal nematodiasis in comparison with the use of the fungus D. flagrans alone. Five groups consisting of eight Girolando heifers were kept in paddocks of Brachiaria decumbens for six months. Each heifer received 1 g/10 kg of pellets containing the fungi (0.2 g of fungus/10 kg b.w.). Group 1 (G1) received pellets with D...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29766518/pls-ipls-ga-pls-models-for-soluble-solids-content-ph-and-acidity-determination-in-intact-dovyalis-fruit-using-near-infrared-spectroscopy
#5
Mateus Wagner Dantas de Assis, Deborah Oliveira De Fusco, Rosangela Câmara Costa, Kássio Michell Gomes de Lima, Luis Carlos Cunha Júnior, Gustavo Henrique de Almeida Teixeira
BACKGROUND: Dovyalis species Dovyalis abyssinica Warb. and Dovyalis hebecarpa Warb. were introduced in Brazil, but the fruit quality of these species are not appropriate for fresh consumption due to the high titratable acidity (TA) and low soluble solids content (SSC). With the selection of new D. abyssinica clones with lower acidity and the hybridization of these two dovyalis specie (D. abyssinica and D. hebecarpa) the fruit quality improved and the better physical-chemical characteristics make them more suitable for fresh consumption...
May 16, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29765499/effect-of-heat-stress-on-sperm-dna-protamine-assessment-in-ram-spermatozoa-and-testicle
#6
Thais Rose Dos Santos Hamilton, Adriano Felipe Perez Siqueira, Letícia Signori de Castro, Camilla Mota Mendes, Juliana de Carvalho Delgado, Patrícia Monken de Assis, Leonardo Pereira Mesquita, Paulo César Maiorka, Marcílio Nichi, Marcelo Demarchi Goissis, José Antonio Visintin, Mayra Elena Ortiz D Ávila Assumpção
Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29730142/implementation-of-tailored-interventions-in-a-statewide-programme-to-reduce-central-line-associated-blood-stream-infections
#7
Denise B Assis, Geraldine Madalosso, Maria Clara Padoveze, Renata D Lobo, Maura S Oliveira, Ícaro Boszczowski, Julio M Singer, Anna S Levin
BACKGROUND: There is a scarcity of studies exploring implementation strategies to central line-associated bloodstream infections (CLABSI) in low or middle income countries. AIM: to implement tailored interventions to reduce CLABSI rates in adult intensive care units (ICU). METHODS: The implementation strategy of the State Health Department was performed in São Paulo State, Brazil over two cycles. Cycle 1 (56 hospitals) was exploratory and Cycle 2 (77 hospitals) was designed to confirm the hypothesis generated by the first cycle, with 3 phases each (pre-intervention, intervention, post-intervention)...
May 3, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29719477/ichthyological-collection-of-the-museu-oceanogr%C3%A3-fico-d-carlos-i
#8
Ana Serra Silva, Maria Pitta Groz, Paula Leandro, Carlos A Assis, Rui Figueira
The collection of the Museu Oceanográfico D. Carlos I is a historical specimen, instrument, and document collection that has been housed at the Aquário Vasco da Gama since 1935. The collection is largely the result of several scientific campaigns conducted by Dom Carlos de Bragança between 1896 and 1907. Specifically, the ichthyological collection consists of 675 surviving catalogue records of specimens caught, acquired or offered to D. Carlos I between 1892 to 1907, and includes the type specimen for Odontaspis nasutus Bragança, 1904 (junior synonym of Mitsukurina owstoni Jordan, 1898), along with several specimens of deep sea species...
2018: ZooKeys
https://www.readbyqxmd.com/read/29704090/association-between-bovine-digital-dermatitis-and-annual-periods-of-lower-pluviometric-precipitation-in-central-brazil
#9
D C Silva, P J B Queiroz, D B S Caetano, B M Assis, R E Rabelo, L A F Silva
We aimed to follow the epidemiologic evolution of bovine digital dermatitis (BDD) and other podal diseases grouped in a dairy farm in Central Brazil between the years 2010 and 2016. This study was carried out in a farm in Jataí, Goiás, Central Brazil, where the prevalence data of bovine digital dermatitis (BDD) and other podal diseases, as well as the composition, history, and management of the herd, was collected. We analyzed the collected data into the two annual pluviometric precipitation values in Central Brazil: rainy season and dry season...
April 27, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29684922/saliva-versus-plasma-therapeutic-drug-monitoring-of-pregabalin-in-jordanian-patients
#10
Nasir Idkaidek, Salim Hamadi, Manal El-Assi, Ahmad Al-Shalalfeh, Ahmad Al-Ghazawi
The objective is using saliva instead of plasma for pregabalin therapeutic drug monitoring (TDM) since saliva reflects the free non-protein bound drug concentration, simple and noninvasive sampling, cheaper and does not require the expertise of drawing blood. Forty four patients participated in this study, two samples of saliva and another two of blood were taken from each patient; first sample of both saliva and blood is the trough sample and was taken just before the first dose of the day and second sample is the peak sample and was taken 1 h after taking the first dose of the day...
April 23, 2018: Drug Research
https://www.readbyqxmd.com/read/29665644/biodistribution-of-free-and-encapsulated-99m-tc-fluconazole-in-an-infection-model-induced-by-candida-albicans
#11
Danielle Nogueira de Assis, Raquel Silva Araújo, Leonardo Lima Fuscaldi, Simone Odília Antunes Fernandes, Vanessa Carla Furtado Mosqueira, Valbert Nascimento Cardoso
BACKGROUND: Candida spp is an etiologic agent of fungal infections in hospitals and resistance to treatment with antifungals has been extensively reported. Thus, it is very important to develop formulations that increase effectiveness with low toxicity. In this sense, nanocarriers have been investigated, once they modify drug biodistribution profile. Thus, this study aimed to evaluate the biodistribution of free and encapsulated 99m Tc-fluconazole into nanocapsules (NCs) in an experimental immunosuppressed murine model of Candida albicans infection...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29659165/improving-academic-performance-of-sport-and-exercise-science-undergraduate-students-in-gross-anatomy-using-a-near-peer-teaching-program
#12
Ricardo Borges Viana, Mário Hebling Campos, Douglas de Assis Teles Santos, Isabela Cristina Maioni Xavier, Rodrigo Luiz Vancini, Marília Santos Andrade, Claudio Andre Barbosa de Lira
Peer and near-peer teaching programs are common in medical undergraduate courses. However, there are no studies that have investigated the effectiveness of a near-peer teaching program on the academic performance of undergraduate students pursuing sport and exercise science coursework. This study was conducted to analyze the effectiveness of such a program for students who participated in a course on the functional anatomy of the locomotor apparatus. A total of 39 student participants were divided into two groups: students in one group voluntarily attended at least one session of a near-peer teaching program, and students in the other group attended no sessions...
April 16, 2018: Anatomical Sciences Education
https://www.readbyqxmd.com/read/29621603/type-c-botulism-in-domestic-chickens-dogs-and-black-pencilled-marmoset-callithrix-penicillata-in-minas-gerais-brazil
#13
Rodrigo Otávio Silveira Silva, Ronaldo Alves Martins, Ronnie Antunes Assis, Carlos Augusto Oliveira Junior, Francisco Carlos Faria Lobato
Botulism is a well-known intoxication that affects humans and animals. The disease is endemic in cattle in Brazil and recently emerged as an important disease in commercial laying hens and broiler chickens in Europe. Dogs and other animal species can also be affected. Although antitoxins are commonly administered to humans diagnosed with botulism, in animals this is rarely the case and the treatment of botulism is still based only on support therapy. In the present work, we report an outbreak of type C botulism in Brazil that simultaneously affected domestic chickens, dogs and a black-pencilled marmoset (Callithrix penicillata)...
April 3, 2018: Anaerobe
https://www.readbyqxmd.com/read/29569995/phosphorylation-and-interactions-associated-with-the-control-of-the-leishmania-poly-a-binding-protein-1-pabp1-function-during-translation-initiation
#14
Osvaldo P de Melo Neto, Tamara D C da Costa Lima, Kleison C Merlo, Tatiany P Romão, Pollyanna O Rocha, Ludmila A Assis, Larissa M Nascimento, Camila C Xavier, Antonio M Rezende, Christian R S Reis, Barbara Papadopoulou
The Poly-A Binding Protein (PABP) is a conserved eukaryotic polypeptide involved in many aspects of mRNA metabolism. During translation initiation, PABP interacts with the translation initiation complex eIF4F and enhances the translation of polyadenylated mRNAs. Schematically, most PABPs can be divided into an N-terminal RNA-binding region, a non-conserved linker segment and the C-terminal MLLE domain. In pathogenic Leishmania protozoans, three PABP homologues have been identified, with the first one (PABP1) targeted by phosphorylation and shown to co-immunoprecipitate with an eIF4F-like complex (EIF4E4/EIF4G3) implicated in translation initiation...
March 23, 2018: RNA Biology
https://www.readbyqxmd.com/read/29521677/biomechanical-behavior-of-tooth-implant-supported-prostheses-with-different-implant-connections-a-nonlinear-finite-element-analysis
#15
Gustavo Assis de Paula, Guilherme Carvalho Silva, Ênio Lacerda Vilaça, Tulimar Machado Cornacchia, Cláudia Silami de Magalhães, Allyson Nogueira Moreira
PURPOSE: Biomechanical behavior of tooth-implant-supported prostheses (TISPs) with external and internal implants was compared. MATERIALS AND METHODS: Two 3-D models of TISP were designed by varying the implant: external (Model EH) and internal hexagons (Model IH). After loading, von Mises stresses were obtained in implants, abutments, and screws. Principal maximum (σmax) and minimum (σmin) stresses were analyzed in periodontal ligament (PL), alveolar bone, and periimplant bone...
March 7, 2018: Implant Dentistry
https://www.readbyqxmd.com/read/29514597/dealing-with-missing-standard-deviation-and-mean-values-in-meta-analysis-of-continuous-outcomes-a-systematic-review
#16
Christopher J Weir, Isabella Butcher, Valentina Assi, Stephanie C Lewis, Gordon D Murray, Peter Langhorne, Marian C Brady
BACKGROUND: Rigorous, informative meta-analyses rely on availability of appropriate summary statistics or individual participant data. For continuous outcomes, especially those with naturally skewed distributions, summary information on the mean or variability often goes unreported. While full reporting of original trial data is the ideal, we sought to identify methods for handling unreported mean or variability summary statistics in meta-analysis. METHODS: We undertook two systematic literature reviews to identify methodological approaches used to deal with missing mean or variability summary statistics...
March 7, 2018: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/29512479/evaluation-of-malaria-urban-risk-using-an-immuno-epidemiological-biomarker-of-human-exposure-to-anopheles-bites
#17
Dipomin F Traoré, André B Sagna, Akré M Adja, Dounin D Zoh, Kouassi N Lingué, Issa Coulibaly, Bertin N'Cho Tchiekoi, Serge B Assi, Anne Poinsignon, Mamadou Dagnogo, Franck Remoue
Urban malaria is an underestimated serious health concern in African countries. This study aimed to evaluate the risk of malaria transmission in an urban area by evaluating the level of human exposure to Anopheles bites using an Anopheles salivary biomarker ( gambiae Salivary Gland Protein-6 peptide 1 [gSG6-P1] peptide). Two multidisciplinary cross-sectional studies were undertaken in five sites of Bouaké city (three urban districts and two surrounding villages, used as control; Côte d'Ivoire) during the rainy season and the dry season...
May 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29497304/insulin-treatment-reverses-the-increase-in-atrogin-1-expression-in-atrophied-skeletal-muscles-of-diabetic-rats-with-acute-joint-inflammation
#18
Clara Maria Pinheiro-Dardis, Vânia Ortega Gutierres, Renata Pires Assis, Sabrina Messa Peviani, Gabriel Borges Delfino, João Luiz Quagliotti Durigan, Tania de Fátima Salvini, Amanda Martins Baviera, Iguatemy Lourenço Brunetti
Background: The aim of this study was to evaluate the changes in biomarkers of skeletal muscle proteolysis (atrogin-1, muscle RING finger-1 protein [MuRF-1]) and inflammation (nuclear factor kappa-B) in skeletal muscles of rats under two catabolic conditions, diabetes mellitus (DM) and acute joint inflammation, and the effects of insulin therapy. Materials and methods: Male Wistar rats were divided into groups without diabetes - normal (N), saline (NS), or ι-carrageenan (NCa) injection into the tibiotarsal joint - and groups with diabetes - diabetes (D), plus insulin (DI), saline (DS), or ι-carrageenan (DCa) injection into the tibiotarsal joint, or ι-carrageenan injection and treatment with insulin (DCaI)...
2018: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/29485675/postpartum-bonding-difficulties-and-adult-attachment-styles-the-mediating-role-of-postpartum-depression-and-childbirth-related-ptsd
#19
Ilana S Hairston, Jonathan E Handelzalts, Chen Assis, Michal Kovo
Despite decades of research demonstrating the role of adult attachment styles and early mother-infant bonding in parenting behaviors and maternal mental health, these constructs have seldom been studied together. The present study aimed to investigate the relationship between attachment styles and specific bonding difficulties of mothers. In addition, as postpartum depression and childbirth-related posttraumatic stress symptoms have been associated with both constructs, we explored their possible mediation effect...
March 2018: Infant Mental Health Journal
https://www.readbyqxmd.com/read/29466761/nkp46-receptor-mediated-interferon-%C3%AE-production-by-natural-killer-cells-increases-fibronectin-1-to-alter-tumor-architecture-and-control-metastasis
#20
Ariella Glasner, Assi Levi, Jonatan Enk, Batya Isaacson, Sergey Viukov, Shari Orlanski, Alon Scope, Tzahi Neuman, Claes D Enk, Jacob H Hanna, Veronika Sexl, Stipan Jonjic, Barbara Seliger, Laurence Zitvogel, Ofer Mandelboim
No abstract text is available yet for this article.
February 20, 2018: Immunity
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