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https://www.readbyqxmd.com/read/29029285/selection-of-reference-genes-for-expression-studies-in-diaphorina-citri-hemiptera-liviidae
#1
Meire Menezes Bassan, Je Ssika Angelotti-Mendonc A, Gustavo Rodrigues Alves, Pedro Takao Yamamoto, Francisco de Assis Alves Moura O Filho
The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is considered the main vector of the bacteria associated with huanglongbing, a very serious disease that has threatened the world citrus industry. The absence of efficient control management protocols, including a lack of resistant cultivars, has led to the development of different approaches to study this pathosystem. The production of resistant genotypes relies on D. citri gene expression analyses by RT-qPCR to assess control of the vector population...
September 27, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29025032/physical-activity-mediating-factors-and-risk-of-colon-cancer-insights-into-adiposity-and-circulating-biomarkers-from-the-epic-cohort
#2
Krasimira Aleksandrova, Mazda Jenab, Michael Leitzmann, Bas Bueno-de-Mesquita, Rudolf Kaaks, Antonia Trichopoulou, Christina Bamia, Pagona Lagiou, Sabina Rinaldi, Heinz Freisling, Marion Carayol, Tobias Pischon, Dagmar Drogan, Elisabete Weiderpass, Paula Jakszyn, Kim Overvad, Christina C Dahm, Anne Tjønneland, Marie-Christine Bouton-Ruault, Tilman Kühn, Eleni Peppa, Elissavet Valanou, Carlo La Vecchia, Domenico Palli, Salvatore Panico, Carlotta Sacerdote, Claudia Agnoli, Rosario Tumino, Anne May, Jonna van Vulpen, Kristin Benjaminsen Borch, Sunday Oluwafemi Oyeyemi, J Ramón Quirós, Catalina Bonet, María-José Sánchez, Miren Dorronsoro, Carmen Navarro, Aurelio Barricarte, Bethany van Guelpen, Patrik Wennberg, Timothy J Key, Kay-Tee Khaw, Nicholas Wareham, Nada Assi, Heather A Ward, Dagfinn Aune, Elio Riboli, Heiner Boeing
Background: There is convincing evidence that high physical activity lowers the risk of colon cancer; however, the underlying biological mechanisms remain largely unknown. We aimed to determine the extent to which body fatness and biomarkers of various biologically plausible pathways account for the association between physical activity and colon cancer. Methods: We conducted a nested case-control study in a cohort of 519 978 men and women aged 25 to 70 years followed from 1992 to 2003...
September 5, 2017: International Journal of Epidemiology
https://www.readbyqxmd.com/read/29021311/acute-ischemic-stroke-with-tandem-lesions-technical-endovascular-management-and-clinical-outcomes-from-the-escape-trial
#3
Zarina Assis, Bijoy K Menon, Mayank Goyal, Andrew M Demchuk, Jai Shankar, Jeremy L Rempel, Daniel Roy, Alexander Y Poppe, Victor Yang, Cheemun Lum, Dar Dowlatshahi, John Thornton, Hana Choe, Paul A Burns, Donald F Frei, Blaise W Baxter, Michael D Hill
BACKGROUND: Tandem occlusions of the extracranial carotid and intracranial carotid or middle cerebral artery have a particularly poor prognosis without treatment. Several management strategies have been used with no clear consensus recommendations. We examined subjects with tandem occlusions enrolled in the ESCAPE trial and their outcomes. METHODS: Data are from the ESCAPE trial. Additional data were sought on interventions for each subject. RESULTS: There were 54 (17%) subjects with tandem extracranial and intracranial occlusions...
October 11, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28982933/steroidogenesis-during-postnatal-testicular-development-of-galea-spixii
#4
P R S Santos, F D Oliveira, M A M Arroyo, M F Oliveira, P Castelucci, A J Conley, A C Assis Neto
The androgen/estrogen balance is essential for normal sexual development and reproduction in mammals. Studies performed herein investigated the potential for estrogen synthesis in cells of the testes of a hystricomorph rodent, Galea spixii The study characterized the expression of the key enzymes responsible for estrogen and androgen synthesis, cytochromes P450 aromatase (P450arom), 17α-hydroxylase/17,20-lyase (P450c17) respectively, as well as the redox partner NADPH cytochrome P450 oxido-reductase (CPR) required to support electron transfer and catalysis of these P450s, by immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR) analysis, throughout postnatal sexual development...
November 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28962655/a-unique-heterologous-fibrin-sealant-hfs-as-a-candidate-biological-scaffold-for-mesenchymal-stem-cells-in-osteoporotic-rats
#5
Patrícia Rodrigues Orsi, Fernanda Cruz Landim-Alvarenga, Luis Antônio Justulin, Ramon Kaneno, Marjorie de Assis Golim, Daniela Carvalho Dos Santos, Camila Fernanda Zorzella Creste, Eunice Oba, Leandro Maia, Benedito Barraviera, Rui Seabra Ferreira
BACKGROUND: The injection of mesenchymal stem cells (MSCs) directly into the bone of osteoporotic (OP) patients for rapid recovery has been studied worldwide. Scaffolds associated with MSCs are used to maintain and avoid cell loss after application. A unique heterologous fibrin sealant (HFS) derived from snake venom was evaluated for the cytotoxicity of its main components and as a three-dimensional biological scaffold for MSCs to repair a critical femur defect in osteoporotic rats. METHODS: The cytotoxicity of HFS was assessed using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay and transmission electron microscopy...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28958178/the-national-health-services-of-brazil-and-northern-europe-universality-equity-and-integrality-time-has-come-for-the-latter
#6
Garibaldi D Gurgel, Islândia M Carvalho de Sousa, Sydia Rosana de Araujo Oliveira, Francisco de Assis da Silva Santos, Finn Diderichsen
In 1990 the national health services in the United Kingdom and Sweden started to split up in internal markets with purchasers and providers. It was also the year when Brazil started to implement a national health service (SUS) inspired by the British national health service that aimed at principles of universality, equity, and integrality. While the reform in Brazil aimed at improving equity and effectiveness, reforms in Europe aimed at improving efficiency in order to contain costs. The European reforms increased supply and utilization but never provided the large increase in efficiency that was hoped for, and inequities have increased...
January 1, 2017: International Journal of Health Services: Planning, Administration, Evaluation
https://www.readbyqxmd.com/read/28950350/a-prospective-evaluation-of-plasma-phospholipid-fatty-acids-and-breast-cancer-risk-in-the-epic-study
#7
V Chajès, N Assi, C Biessy, P Ferrari, S Rinaldi, N Slimani, G M Lenoir, L Baglietto, M His, M C Boutron-Ruault, A Trichopoulou, P Lagiou, M Katsoulis, R Kaaks, T Kühn, S Panico, V Pala, G Masala, H B Bueno-de-Mesquita, P H Peeters, C van Gils, A Hjartåker, Standahl K Olsen, Borgund R Barnung, A Barricarte, D Redondo- Sanchez, V Menéndez, P Amiano, M Wennberg, T Key, K T Khaw, M A Merritt, E Riboli, M J Gunter, I Romieu
Background: Intakes of specific fatty acids have been postulated to impact breast cancer risk but epidemiological data based on dietary questionnaires remain conflicting. Material and methods: We assessed the association between plasma phospholipid fatty acids and breast cancer risk in a case-control study nested within the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Sixty fatty acids were measured by gas chromatography in pre-diagnostic plasma phospholipids from 2,982 incident breast cancer cases matched to 2,982 controls...
September 18, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28943398/cold-sensing-trpm8-channel-participates-in-circadian-control-of-the-brown-adipose-tissue
#8
Maria Nathália Moraes, Leonardo Vinicius Monteiro de Assis, Felipe Dos Santos Henriques, Miguel Luiz Batista, Ali D Güler, Ana Maria de Lauro Castrucci
Transient receptor potential (TRP) channels are known to regulate energy metabolism, and TRPM8 has become an interesting player in this context. Here we demonstrate the role of the cold sensor TRPM8 in the regulation of clock gene and clock controlled genes in brown adipose tissue (BAT). We investigated TrpM8 temporal profile in the eyes, suprachiasmatic nucleus and BAT; only BAT showed temporal variation of TrpM8 transcripts. Eyes from mice lacking TRPM8 lost the temporal profile of Per1 in LD cycle. This alteration in the ocular circadian physiology may explain the delay in the onset of locomotor activity in response to light pulse, as compared to wild type animals (WT)...
September 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28920893/a-functional-genetic-scheme-for-seizure-forecasting-in-canine-epilepsy
#9
E Bou Assi, D K Nguyen, S Rihana, M Sawan
OBJECTIVE: The objective of this work is the development of an accurate seizure forecasting algorithm that considers brain's functional connectivity for electrode selection. METHODS: We start by proposing Kmeans-directed transfer function, an adaptive functional connectivity method intended for seizure onset zone localization in bilateral intracranial EEG recordings. Electrodes identified as seizure activity sources and sinks are then used to implement a seizure-forecasting algorithm on long-term continuous recordings in dogs with naturallyoccurring epilepsy...
September 13, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28913904/re-emergence-of-rabies-virus-maintained-by-canid-populations-in-paraguay
#10
A C F Amarilla, J C A Pompei, D B Araujo, F A Vázquez, R R Galeano, L M Delgado, G Bogado, M Colman, L Sanabria, K Iamamoto, R Garcia, D Assis, R Recalde, L F Martorelli, E Quiñones, A Cabello, M Martini, O Cosivi, E L Durigon, S R Favoretto
Paraguay has registered no human cases of rabies since 2004, and the last case in dogs, reported in 2009, was due to a variant maintained in the common vampire bat "Desmodus rotundus". In 2014, a dog was diagnosed as positive for rabies with aggression towards a boy and all required measures of control were successfully adopted. Epidemiological investigation revealed that the dog was not vaccinated and had been attacked by a crab-eating fox, "zorro" (Cerdocyon thous). The sample was diagnosed by the Official Veterinary Service of the Country and sent to the Center on Rabies Research from the University of São Paulo, Brazil, for antigenic and genetic characterization...
September 14, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28860631/evidence-for-rangewide-panmixia-despite-multiple-barriers-to-dispersal-in-a-marine-mussel
#11
Carla R Lourenço, Katy R Nicastro, Christopher D McQuaid, Rosa M Chefaoui, Jorge Assis, Mohammed Z Taleb, Gerardo I Zardi
Oceanographic features shape the distributional and genetic patterns of marine species by interrupting or promoting connections among populations. Although general patterns commonly arise, distributional ranges and genetic structure are species-specific and do not always comply with the expected trends. By applying a multimarker genetic approach combined with Lagrangian particle simulations (LPS) we tested the hypothesis that oceanographic features along northeastern Atlantic and Mediterranean shores influence dispersal potential and genetic structure of the intertidal mussel Perna perna...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28832025/corrigendum-whole-genome-analysis-of-a-schistosomiasis-transmitting-freshwater-snail
#12
Coen M Adema, LaDeana W Hillier, Catherine S Jones, Eric S Loker, Matty Knight, Patrick Minx, Guilherme Oliveira, Nithya Raghavan, Andrew Shedlock, Laurence Rodrigues do Amaral, Halime D Arican-Goktas, Juliana G Assis, Elio Hideo Baba, Olga L Baron, Christopher J Bayne, Utibe Bickham-Wright, Kyle K Biggar, Michael Blouin, Bryony C Bonning, Chris Botka, Joanna M Bridger, Katherine M Buckley, Sarah K Buddenborg, Roberta Lima Caldeira, Julia Carleton, Omar S Carvalho, Maria G Castillo, Iain W Chalmers, Mikkel Christensens, Sandra Clifton, Celine Cosseau, Christine Coustau, Richard M Cripps, Yesid Cuesta-Astroz, Scott F Cummins, Leon Di Stefano, Nathalie Dinguirard, David Duval, Scott Emrich, Cédric Feschotte, Rene Feyereisen, Peter FitzGerald, Catrina Fronick, Lucinda Fulton, Richard Galinier, Sandra G Gava, Michael Geusz, Kathrin K Geyer, Gloria I Giraldo-Calderón, Matheus de Souza Gomes, Michelle A Gordy, Benjamin Gourbal, Christoph Grunau, Patrick C Hanington, Karl F Hoffmann, Daniel Hughes, Judith Humphries, Daniel J Jackson, Liana K Jannotti-Passos, Wander de Jesus Jeremias, Susan Jobling, Bishoy Kamel, Aurélie Kapusta, Satwant Kaur, Joris M Koene, Andrea B Kohn, Dan Lawson, Scott P Lawton, Di Liang, Yanin Limpanont, Sijun Liu, Anne E Lockyer, Ty Anna L Lovato, Fernanda Ludolf, Vince Magrini, Donald P McManus, Monica Medina, Milind Misra, Guillaume Mitta, Gerald M Mkoji, Michael J Montague, Cesar Montelongo, Leonid L Moroz, Monica C Munoz-Torres, Umar Niazi, Leslie R Noble, Francislon S Oliveira, Fabiano S Pais, Anthony T Papenfuss, Rob Peace, Janeth J Pena, Emmanuel A Pila, Titouan Quelais, Brian J Raney, Jonathan P Rast, David Rollinson, Izinara C Rosse, Bronwyn Rotgans, Edwin J Routledge, Kathryn M Ryan, Larissa L S Scholte, Kenneth B Storey, Martin Swain, Jacob A Tennessen, Chad Tomlinson, Damian L Trujillo, Emanuela V Volpi, Anthony J Walker, Tianfang Wang, Ittiprasert Wannaporn, Wesley C Warren, Xiao-Jun Wu, Timothy P Yoshino, Mohammed Yusuf, Si-Ming Zhang, Min Zhao, Richard K Wilson
This corrects the article DOI: 10.1038/ncomms15451.
August 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28809857/grandmaternal-stress-during-pregnancy-and-dna-methylation-of-the-third-generation-an-epigenome-wide-association-study
#13
F Serpeloni, K Radtke, S G de Assis, F Henning, D Nätt, T Elbert
Stress during pregnancy may impact subsequent generations, which is demonstrated by an increased susceptibility to childhood and adulthood health problems in the children and grandchildren. Although the importance of the prenatal environment is well reported with regards to future physical and emotional outcomes, little is known about the molecular mechanisms that mediate the long-term consequences of early stress across generations. Recent studies have identified DNA methylation as a possible mediator of the impact of prenatal stress in the offspring...
August 15, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28805476/microencapsulate-aspergillus-niger-peptidases-from-agroindustrial-waste-wheat-bran-spray-process-evaluation-and-stability
#14
T P F Cabral, N C Bellini, K R Assis, C C C Teixeira, A D Lanchote, H Cabral, L A P de Freitas
The aim of this work was to obtain microencapsulated stable Aspergillus niger peptidases by post fermentation spray drying. The enzymatic extract was evaluated before and after spray drying microencapsulation to verify the effects of five different process parameters on the extract enzymatic activity, i.e. air flow, extract feed rate, drying temperature, homogenizing time and weight ratio of extract to encapsulation material. The optimal conditions were determined by desirability functions and experimentally confirmed...
August 14, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28791487/metformin-attenuates-the-tlr4-inflammatory-pathway-in-skeletal-muscle-of-diabetic-rats
#15
Leonardo Gomes Peixoto, Renata Roland Teixeira, Danielle Diniz Vilela, Lara Naves Barbosa, Douglas Carvalho Caixeta, Simone Ramos Deconte, Fernanda de Assis de Araújo, Robinson Sabino-Silva, Foued Salmen Espindola
AIMS: Inflammation induced by hyperglycemia triggers the toll-like receptor (TLR) pathway into cells. Our hypothesis was that metformin treatment attenuates the TLR signaling pathways triggered by inflammation in skeletal muscle of hypoinsulinemic/hyperglycemic STZ-induced rats. Thus, we examined TLR signaling under hypoinsulinemia and hyperglycemia conditions and its correlation with insulin resistance in muscle of diabetic rats treated with metformin. METHODS: Ten-day diabetic rats were submitted to 7 days of saline (D group) or metformin (500 mg/kg once per day) (D + M group)...
August 9, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/28776029/genome-wide-signatures-of-complex-introgression-and-adaptive-evolution-in-the-big-cats
#16
Henrique V Figueiró, Gang Li, Fernanda J Trindade, Juliana Assis, Fabiano Pais, Gabriel Fernandes, Sarah H D Santos, Graham M Hughes, Aleksey Komissarov, Agostinho Antunes, Cristine S Trinca, Maíra R Rodrigues, Tyler Linderoth, Ke Bi, Leandro Silveira, Fernando C C Azevedo, Daniel Kantek, Emiliano Ramalho, Ricardo A Brassaloti, Priscilla M S Villela, Adauto L V Nunes, Rodrigo H F Teixeira, Ronaldo G Morato, Damian Loska, Patricia Saragüeta, Toni Gabaldón, Emma C Teeling, Stephen J O'Brien, Rasmus Nielsen, Luiz L Coutinho, Guilherme Oliveira, William J Murphy, Eduardo Eizirik
The great cats of the genus Panthera comprise a recent radiation whose evolutionary history is poorly understood. Their rapid diversification poses challenges to resolving their phylogeny while offering opportunities to investigate the historical dynamics of adaptive divergence. We report the sequence, de novo assembly, and annotation of the jaguar (Panthera onca) genome, a novel genome sequence for the leopard (Panthera pardus), and comparative analyses encompassing all living Panthera species. Demographic reconstructions indicated that all of these species have experienced variable episodes of population decline during the Pleistocene, ultimately leading to small effective sizes in present-day genomes...
July 2017: Science Advances
https://www.readbyqxmd.com/read/28758666/maldi-ms-imaging-investigation-of-the-host-response-to-visceral-leishmaniasis
#17
C F Jaegger, F Negrão, D M Assis, K R A Belaz, C F F Angolini, A M A P Fernandes, V G Santos, A Pimentel, D R Abánades, S Giorgio, M N Eberlin, D F O Rocha
Mass spectrometry imaging (MSI) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. Leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. Herein we have employed MALDI MSI to examine peptides and low molecular weight proteins (2 to 20 kDa) differentially expressed in the liver during visceral leishmaniasis in mice models...
September 26, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28756916/effect-of-dietary-supplementation-with-olive-and-sunflower-oils-on-lipid-profile-and-liver-histology-in-rats-fed-high-cholesterol-diet
#18
Sandra Mara Pimentel Duavy, Gerson Javier Torres Salazar, Gerlânia de Oliveira Leite, Assis Ecker, Nilda Vargas Barbosa
OBJECTIVE: To compare the effects of high-monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) against the metabolic disorders elicited by a high-cholesterol diet (HC) in rats. METHODS: Using in vivo dietary manipulation, rats were fed with different diets containing 4% soybean oil (cholesterol free diet) and 1% HC containing 12% olive oil (HC + OO) enriched with MUFA and 12% sunflower oil (HC + SO) enriched with PUFA for 60 d. Serum lipid levels and hepatic steatosis were evaluated after the treatment period...
June 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28754099/antioxidant-and-vasorelaxant-activities-induced-by-northeastern-brazilian-fermented-grape-skins
#19
José George F Albuquerque, Valéria L Assis, Arthur J P O Almeida, Ionaldo J L D Basílio, Melissa N Luciano, Bruno R L A Meireles, Ângela M T M Cordeiro, Islânia G A Araújo, Robson C Veras, Thaís P Ribeiro, Isac A Medeiros
BACKGROUND: In northeastern Brazil, grape pomace has become a potential alternative byproduct because of the recover phenolic compounds from the vinification process. Comparative analyses were performed between lyophilized extract of grape skins from pomace, described as fermented (FGS), and fresh, unfermented (UGS) grape skins to show the relevant brand's composition upon the first maceration in winemaking. METHODS: The use of in vitro testing such as Folin-Ciocalteu's, DPPH free radical scavenger and HPLC methods were performed to evidence antioxidant effect and phenolic compounds...
July 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28695729/semi-integrative-voltammetry-as-an-efficient-tool-to-study-simple-electrochemical-systems-in-deep-eutectic-solvents
#20
Alan M Perdizio Sakita, Rodrigo Della Noce, Cecílio S Fugivara, Assis V Benedetti
The utilization of deep eutectic solvents (DESs) in electrochemical studies has grown in recent years due to their low cost and environmental friendliness compared to traditional ionic liquids. However, the determination of diffusion coefficient (D) and heterogeneous constant rate (k(0)) using the traditional electrochemical approach may mask some concerns because of the high viscosity and limited conductivity in these media. In this work, it is shown that the determinations of D and k(0) in glyceline DES by voltammetric studies using the Nicholson approach is problematic...
July 26, 2017: Analytical Chemistry
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