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Wound imaging

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https://www.readbyqxmd.com/read/28528048/real-time-autofluorescence-imaging-to-diagnose-lvad-driveline-infections
#1
Jack B Keenan, Taufiek Konrad Rajab, David G Armstrong, Zain Khalpey
A 64-year-old man experienced a driveline infection that was treated with serial debridements and antibiotics. When the wound clinically appeared ready for closure, a handheld fluorescence imaging device still revealed a margin of red fluorescence around the wound edges consistent with a subclinical infection. Therefore, a wider margin was made and additional specimens for wound culture were taken, which demonstrated a vancomycin-resistant enterococcal infection. The autofluorescence signals of common bacteria can be detected with a fluorescence camera in subclinical wound infections without clinical signs...
June 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28523497/maldi-msi-of-lipids-in-human-skin
#2
Philippa J Hart, Malcolm R Clench
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28522300/primary-reconstructive-method-for-tracheal-defect-from-invasion-by-differentiated-thyroid-carcinoma
#3
Atsumori Hamahata, Takeshi Beppu, Takashi Yamaki, Hiroyuki Sakurai
Differentiated thyroid carcinoma is not highly malignant, and thus surgical resection is the most common treatment even if the carcinoma has invaded the trachea. Although some cases exist in which the carcinoma invades the trachea, using the tracheal window resection method allows complete resection of the carcinoma. Yet these patients must often control a large tracheocutaneous wound until reconstructive surgery is performed because such surgery occurs secondarily. Our hospital admitted three patients for which tracheally invasive, differentiated thyroid carcinoma was surgically resected...
May 15, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28516727/non-invasive-longitudinal-monitoring-of-angiogenesis-in-a-murine-full-thickness-cutaneous-wound-healing-model-using-high-resolution-three-dimensional-ultrasound-imaging
#4
K Mukai, W Zhu, Y Nakajima, M Kobayashi, T Nakatani
BACKGROUND/PURPOSE: The aim of this study was to evaluate the longitudinal monitoring of angiogenesis in a murine full-thickness cutaneous wound healing model using high-resolution three-dimensional (3D) ultrasound imaging. METHODS: Two C57BL/6 mice were used. Two-dimensional (2D) ultrasound images with the Color Doppler mode were acquired at regular spatial intervals on day 9, 11, and 14 after wounding. 3D ultrasound images were processed by reconstructing the 2D ultrasound image sequences...
May 17, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/28515948/cerebral-air-embolism-associated-with-penetrating-lung-injury-a-case-report-and-review-of-the-literature
#5
Ayumu Yamaoka, Kei Miyata, Eichi Narimatsu, Eiji Sakawaki, Sonoko Sakawaki, Suguru Hirayama, Shuji Uemura, Naoya Yama
CASE: A 44-year-old man intentionally stabbed himself in the anterior neck and left thorax with a fruit knife. Physical examination revealed two open wounds entering the thoracic cavity in the front chest, and a stab wound entering the trachea at the neck. Two chest tubes were initially inserted for the left lung injury with open hemopneumothorax. Nevertheless, the worsening oxygenation required positive pressure ventilation (PPV) with endotracheal intubation. OUTCOME: Right hemiparesis was found during weaning from PPV...
April 2017: Acute Medicine & Surgery
https://www.readbyqxmd.com/read/28512163/retroperitoneal-laparoscopic-radical-nephrectomy-allows-continuation-of-peritoneal-dialysis-in-patients-with-end-stage-renal-disease
#6
Aziz Khambati, Mary Kate Fitzgerald, Kent T Perry, Robert B Nadler
Patient outcomes for retroperitoneal laparoscopic radical nephrectomy (RLRN) in adults on peritoneal dialysis (PD) have not been described in the literature. This is the first series on the successful application of RLRN for renal cell carcinoma (RCC) among adults with end-stage renal disease (ESRD) on PD. In our series of 5 patients, all were able to promptly resume PD, no wound complications were noted at postoperative visits, and no evidence of recurrence was identified on surveillance imaging at follow-up...
May 2017: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/28511700/a-new-easy-to-make-pectin-honey-hydrogel-enhances-wound-healing-in-rats
#7
Gessica Giusto, Cristina Vercelli, Francesco Comino, Vittorio Caramello, Massimiliano Tursi, Marco Gandini
BACKGROUND: Honey, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of the present study was to develop and evaluate a pectin-honey hydrogel (PHH) as a wound healing membrane and to compare this dressing to liquid honey. METHODS: Thirty-six adult male Sprague-Dawley rats were anesthetized and a 2 × 2 cm excisional wound was created on the dorsum...
May 16, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28508466/comparison-of-the-time-taken-for-localised-breast-surgery-pre-and-post-introduction-of-intra-operative-digital-specimen-mammography
#8
Vinicius Carraro do Nascimento, Anita G Bourke
INTRODUCTION: More than half of the patients with an impalpable malignant breast lesion have a mammographically detected and imaged-guided localisation, which can be technically challenging for the breast surgeon. Specimen imaging is used to confirm successful excision of the localised index lesion and has improved the operating list efficiency resulting in a higher number of excisions per surgical list. The aim of this study was to evaluate whether introducing IDSM (intra-operative digital specimen mammography) saved operation time for localised breast surgery...
May 16, 2017: Journal of Medical Imaging and Radiation Oncology
https://www.readbyqxmd.com/read/28505080/first-insights-into-human-fingertip-regeneration-by-echo-doppler-imaging-and-wound-microenvironment-assessment
#9
Paris Jafari, Camillo Muller, Anthony Grognuz, Lee Ann Applegate, Wassim Raffoul, Pietro G di Summa, Sébastien Durand
Fingertip response to trauma represents a fascinating example of tissue regeneration. Regeneration derives from proliferative mesenchymal cells (blastema) that subsequently differentiate into soft and skeletal tissues. Clinically, conservative treatment of the amputated fingertip under occlusive dressing can shift the response to tissue loss from a wound repair process towards regeneration. When analyzing by Immunoassay the wound exudate from occlusive dressings, the concentrations of brain-derived neurotrophic factor (BDNF) and leukemia inhibitory factor (LIF) were higher in fingertip exudates than in burn wounds (used as controls for wound repair versus regeneration)...
May 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28503467/medial-lemniscus-tract-lesion-after-high-voltage-electrical-injury-a-case-report
#10
Chul-Hyun Cho, Dong Gyu Lee
We present the case of a 33-year-old man who experienced a 10,000-V electrical shock when working with electrical wiring. He suffered third-degree burns on his scalp at the right occiput (entry wound) and on his left arm (exit would), and a second-degree burn on his left foot (exit wound). He presented with severe spasticity of both lower extremities, motor weakness with a Medical Research Council grade of 3, and sensory impairments below thoracic level 11 that included an inability to sense light touch and defects in proprioception...
April 2017: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/28497448/healing-of-spongiosus-cutaneous-fistula-with-hyperbaric-oxygen-therapy-hbot-a-case-report
#11
Pier A Della Camera, Gianmartin Cito, Simone Morselli, Francesco Sessa, Davide Facchiano, Andrea Cocci, Mauro Gacci, Sergio Serni, Marco Carini, Alessandro Natali
INTRODUCTION: We present a case of a spongiosus-cutaneous fistula in a 39-year-old man with recurrent episodes of cutaneous abscess in dorsal middle third penis (5 × 3 cm) treated with Hyperbaric Oxygen Therapy (HBOT). CASE REPORT: After emptying nodular abscess, the patient was noncompliant for further surgery. Therefore, it was suggested the association between HBOT and antibiotic therapy. HBOT is carried out in a hyperbaric room, where the internal pressure is increased (compression phase) by entering compressed air up to 283...
May 10, 2017: Urologia
https://www.readbyqxmd.com/read/28495536/neoaortoiliac-system-procedure-to-treat-infected-aortic-grafts
#12
Chris Klonaris, Nikolaos Patelis, Athanasios Katsargyris, Dimitrios Athanasiadis, Andreas Alexandrou, Theodoros Liakakos
OBJECTIVE: The aim of this case series is to report the results of our centre in the surgical treatment of infected abdominal aortic grafts using the neo-aortoiliac system (NAIS) procedure. PATIENTS AND METHODS: Four male and two female patients underwent a NAIS repair after endovascular (n=3), open (n=2) or combined surgery (n=1) in our centre. Mean age was 73±5.2 years. Graft infection was diagnosed by computed tomography angiography (CTA) and only in two cases further imaging was necessary...
May 8, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28494970/image-based-measurement-of-h2o2-reaction-diffusion-in-wounded-zebrafish-larvae
#13
Mark Jelcic, Balázs Enyedi, João B Xavier, Philipp Niethammer
Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury site. How Duox establishes long-range signaling to leukocytes is unclear. We conceived that extracellular hydrogen peroxide (H2O2) generated by Duox diffuses through the tissue to directly regulate chemotactic signaling in these cells...
May 9, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28492406/routine-computed-tomography-after-recent-operative-exploration-for-penetrating-trauma-what-injuries-do-we-miss
#14
April E Mendoza, Christopher A Wybourn, Anthony G Charles, Andre Campbell, Bruce A Cairns, Margaret M Knudson
BACKGROUND: Patients with penetrating trauma who cannot be stabilized undergo operative intervention without preoperative imaging. In such cases, post-operative imaging may reveal additional injuries not identified during the initial operative exploration. The purpose of this study is to explore the utility of post-operative CT imaging in the setting of penetrating trauma. METHODS: This was a retrospective analysis of patients with penetrating trauma treated at an urban level 1 trauma center between 2010-2015...
May 11, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28492199/an-infant-case-of-intraoral-penetrating-injury-with-a-toothbrush-causing-retropharyngeal-and-upper-mediastinal-emphysema
#15
Kei Jitsuiki, Ayako Hashimoto, Toshihiko Yoshizawa, Youichi Yanagawa
A 2-year-old male child who was running and fell with a toothbrush in his mouth suffered an injury to the inside of his right cheeks. His mother noticed that the toothbrush had impaled his mouth, and removed it. On arrival, a wound was observed on the right cheeks and palate. His general condition was good. However, computed tomography revealed emphysema from the left side of epipharynx to the upper mediastinum. This patient shows the importance of not only checking the medical history and performing a physical examination but also performing an imaging examination...
April 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28491036/pharmacological-activity-and-clinical-use-of-pdrn
#16
REVIEW
Francesco Squadrito, Alessandra Bitto, Natasha Irrera, Gabriele Pizzino, Giovanni Pallio, Letteria Minutoli, Domenica Altavilla
PDRN is a proprietary and registered drug that possesses several activities: tissue repairing, anti-ischemic, and anti-inflammatory. These therapeutic properties suggest its use in regenerative medicine and in diabetic foot ulcers. PDRN holds a mixture of deoxyribonucleotides with molecular weights ranging between 50 and 1,500 KDa, it is derived from a controlled purification and sterilization process of Oncorhynchus mykiss (Salmon Trout) or Oncorhynchus keta (Chum Salmon) sperm DNA. The procedure guarantees the absence of active protein and peptides that may cause immune reactions...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28490473/specific-imaging-of-bacterial-infection-using-6-18-f-fluoromaltotriose-a-second-generation-pet-tracer-targeting-the-maltodextrin-transporter-in-bacteria
#17
Gayatri Gowrishankar, Jonathan Hardy, Mirwais Wardak, Mohammad Namavari, Robert Reeves, Evgenios Neofytou, Ananth Srinivasan, Joseph Wu, Christopher Contag, Sanjiv Gambhir
Purpose: 6"-(18)F-fluoromaltotriose is a novel positron emission tomography (PET) tracer that can potentially be used to image and localize most bacterial infections, much like 2-deoxy-2-(18)F-fluoro-D-glucose ((18)F-FDG) has been used to image and localize many cancers. However, unlike (18)F-FDG, 6"-(18)F-fluoromaltotriose is not taken up by inflammatory lesions and appears to be specific to bacterial infections by targeting the maltodextrin transporter that is expressed in most Gram-positive and Gram-negative strains of bacteria...
May 10, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28486563/in-vitro-induction-of-netosis-comprehensive-live-imaging-comparison-and-systematic-review
#18
Tamara Hoppenbrouwers, Anouchska S A Autar, Andi R Sultan, Tsion E Abraham, Wiggert A van Cappellen, Adriaan B Houtsmuller, Willem J B van Wamel, Heleen M M van Beusekom, Johan W van Neck, Moniek P M de Maat
BACKGROUND: Multiple inducers of in vitro Neutrophil Extracellular Trap (NET) formation (NETosis) have been described. Since there is much variation in study design and results, our aim was to create a systematic review of NETosis inducers and perform a standardized in vitro study of NETosis inducers important in (cardiac) wound healing. METHODS: In vitro NETosis was studied by incubating neutrophils with PMA, living and dead bacteria (S. aureus and E. coli), LPS, (activated) platelets (supernatant), glucose and calcium ionophore Ionomycin using 3-hour periods of time-lapse confocal imaging...
2017: PloS One
https://www.readbyqxmd.com/read/28483547/changes-in-emergency-department-imaging-perspectives-from-national-patient-surveys-over-two-decades
#19
Andrew B Rosenkrantz, Tarek N Hanna, James S Babb, Richard Duszak
PURPOSE: To use patient-generated data to assess the changing role of emergency department (ED) imaging for a spectrum of clinical indications. METHODS: The Household Component Emergency Room Visits File was obtained from 1996 through 2014 for the Medical Expenditure Panel Survey, a nationally representative survey of US households. Percentage of visits associated with various imaging modalities was computed annually, stratified by respondents' self-reported primary condition during the visit...
May 5, 2017: Journal of the American College of Radiology: JACR
https://www.readbyqxmd.com/read/28482625/transepidermal-oxygen-flux-during-arterial-occlusion-using-ratiometric-luminescence-imaging
#20
M Ranieri, S Klein, A Kotrade, C Taeger, J D Dolderer, L Prantl, S Geis
BACKGROUND: A physiological oxygen transport through a circulatory and microcirculatory system is essential for execution of cellular functions. Several pathological conditions e.g. infections, ischemia, cancer, diabetes, hypertension or chronic wounds show a change of oxygen distribution and oxygen tension in cellular microenvironment. Additionally complex operative procedures in order to reconstruct tissue defects require a reliable monitoring of microcirculation. OBJECTIVE: Target of this study was to evaluate skin oxygenation during an ischemia-reperfusion experiment using transepidermal oxygen flux imaging...
May 2, 2017: Clinical Hemorheology and Microcirculation
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