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Xiaoling Wang

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https://www.readbyqxmd.com/read/28339076/an-activatable-aptamer-based-fluorescence-probe-for-the-detection-of-hepg2-cells
#1
Zongqiang Lai, Juntao Tan, Ruirong Wan, Jie Tan, Zhenghua Zhang, Zixi Hu, Jieping Li, Wei Yang, Yiwei Wang, Yafeng Jiang, Jian He, Nuo Yang, Xiaoling Lu, Yongxiang Zhao
It is significant to develop a probe with sensitivity and specificity for the detection of cancer cells. The present study aimed to develop an 'activatable' aptamer-based fluorescence probe (AAFP) to detect cancer cells and frozen cancer tissue. This AAFP consisted of two fragments: aptamer TLS11a that targets HepG2 cells, and two short extending complementary DNA sequences with a 5'- and 3'-terminus that make the aptamer in hairpin structure a capable quencher to fluorophore. The ability of the AAFP to bind specifically to cancer cells was assessed using flow cytometry, fluorescence spectroscopy and fluorescence microscopy...
March 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28336946/real-time-observational-evidence-of-changing-asian-dust-morphology-with-the-mixing-of-heavy-anthropogenic-pollution
#2
Xiaole Pan, Itsushi Uno, Zhe Wang, Tomoaki Nishizawa, Nobuo Sugimoto, Shigekazu Yamamoto, Hiroshi Kobayashi, Yele Sun, Pingqing Fu, Xiao Tang, Zifa Wang
Natural mineral dust and heavy anthropogenic pollution and its complex interactions cause significant environmental problems in East Asia. Due to restrictions of observing technique, real-time morphological change in Asian dust particles owing to coating process of anthropogenic pollutants is still statistically unclear. Here, we first used a newly developed, single-particle polarization detector and quantitatively investigate the evolution of the polarization property of backscattering light reflected from dust particle as they were mixing with anthropogenic pollutants in North China...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28320554/pregnancy-and-kidney-outcomes-in-patients-with-iga-nephropathy-a-cohort-study
#3
Xiaole Su, Jicheng Lv, Youxia Liu, Jinwei Wang, Xinxin Ma, Sufang Shi, Lijun Liu, Hong Zhang
BACKGROUND: The outcomes of pregnancy in immunoglobulin A nephropathy (IgAN) are controversial. This cohort study assessed the effects of pregnancy on kidney disease progression and risk factors for adverse pregnancy outcomes in patients with IgAN. STUDY DESIGN: A cohort study. SETTING & PARTICIPANTS: Women of child-bearing age with IgAN and minimum follow-up of 1 year after biopsy from December 2003 to September 2014. PREDICTORS: Pregnancy, treated as a time-dependent variable; baseline (at time of biopsy) estimated glomerular filtration rate (eGFR), proteinuria, blood pressure, and kidney pathology (Oxford MEST classification)...
March 17, 2017: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
https://www.readbyqxmd.com/read/28319768/quantitative-evaluation-of-the-interfacial-interactions-between-a-randomly-rough-sludge-floc-and-membrane-surface-in-a-membrane-bioreactor-based-on-fractal-geometry
#4
Meijia Zhang, Xiaoling Zhou, Liguo Shen, Xiang Cai, Fangyuan Wang, Jianrong Chen, Hongjun Lin, Renjie Li, Xilin Wu, Bao-Qiang Liao
In this paper, a new method for quantification of interfacial interactions between a randomly rough particle and membrane surface was proposed. It was found that sludge flocs in a membrane bioreactor were of apparent fractal characteristics, and could be modeled by the modified two-variable Weierstrass-Mandelbrot (WM) function. By combining the surface element integration (SEI) method, differential geometry and composite Simpson's rule, the quantitation method for calculating such interfacial interactions was further developed...
March 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28300149/corrigendum-innate-positive-chemotaxis-to-pollen-from-crops-and-banker-plants-in-predaceous-biological-control-agents-towards-new-field-lures
#5
Shu Li, Xiaoling Tan, Nicolas Desneux, Giovanni Benelli, Jing Zhao, Xinhai Li, Fan Zhang, Xiwu Gao, Su Wang
No abstract text is available yet for this article.
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28271709/comparative-analysis-of-recombinant-cytochrome-p450-cyp9a61-from-cydia-pomonella-expressed-in-escherichia-coli-and-pichia-pastoris
#6
Xue-Qing Yang, Wei Wang, Xiao-Ling Tan, Xiao-Qi Wang, Hui Dong
On the basis of prior work, cytochrome P450 CYP9A61 was found to be enriched in fat bodies and during feeding stages, and transcription was induced by λ-cyhalothrin in Cydia pomonella. In this study, recombinant CYP9A61 was expressed in Escherichia coli and Pichia pastoris, and its biochemical properties were investigated. Substrate saturation curves and biochemical properties revealed that, in the presence of glycosylation, the yeast-secreted CYP9A61 exhibited a higher affinity for the substrate p-nitroanisole and was found to be more stable at certain pHs and temperatures than bacterially produced CYP9A61...
March 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28263278/international-nursing-research-on-the-correlation-between-empathy-and-china-s-big-five-personality-theory-implications-for-nursing-leaders
#7
Cong Wang, Qin Wu, Mei Feng, Qunfang Wan, Xiaoling Wu
To investigate the characteristics of nurses' empathy and explore the correlation between nurses' empathy and personality, a cross-sectional study with 250 nurses from a general hospital in China was conducted using the Chinese Big Five Personality Inventory (CBF-PI) and the Jefferson Scale of Empathy-Health Professionals (JSE-HP). The total score of the JSE-HP was 110.60 (SD = 11.71). Employment forms and child-rearing situations were the significant predictors of the JSE-HP score. Multiple hierarchical regression analysis indicated that the JSE-HP score was positively correlated with conscientiousness and agreeableness and the contribution of CBF-PI to JSE-HP scale variances was 15...
April 2017: Nursing Administration Quarterly
https://www.readbyqxmd.com/read/28258923/cancer-associated-fibroblasts-promote-irradiated-cancer-cell-recovery-through-autophagy
#8
Yongbin Wang, Guifang Gan, Bocheng Wang, Jinliang Wu, Yuan Cao, Dan Zhu, Yan Xu, Xiaona Wang, Hongxiu Han, Xiaoling Li, Ming Ye, Jiangmin Zhao, Jun Mi
Tumor relapse after radiotherapy is a significant challenge to oncologists, even after recent the advances in technologies. Here, we showed that cancer-associated fibroblasts (CAFs), a major component of cancer stromal cells, promoted irradiated cancer cell recovery and tumor relapse after radiotherapy. We provided evidence that CAFs-produced IGF1/2, CXCL12 and β-hydroxybutyrate were capable of inducing autophagy in cancer cells post-radiation and promoting cancer cell recovery from radiation-induced damage in vitro and in vivo in mice...
February 22, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28257107/micronutrient-fortified%C3%A2-milk%C3%A2-and%C3%A2-academic%C3%A2-performance%C3%A2-among%C3%A2-chinese%C3%A2-middle%C3%A2-school%C3%A2-students-%C3%A2-a%C3%A2-cluster-randomized%C3%A2-controlled%C3%A2-trial
#9
Xiaoqin Wang, Zhaozhao Hui, Xiaoling Dai, Paul D Terry, Yue Zhang, Mei Ma, Mingxu Wang, Fu Deng, Wei Gu, Shuangyan Lei, Ling Li, Mingyue Ma, Bin Zhang
Many children suffer from nutritional deficiencies that may negatively affect their academic performance. This cluster-randomized controlled trial aimed to test the effects of micronutrient-fortified milk in Chinese students. Participants received either micronutrient-fortified (n = 177) or unfortified (n = 183) milk for six months. Academic performance, motivation, and learning strategies were estimated by end-of-term tests and the Motivated Strategies for Learning Questionnaire. Blood samples were analyzed for micronutrients...
March 2, 2017: Nutrients
https://www.readbyqxmd.com/read/28255111/-sirtuin-type-1-signaling-pathway-mediates-the-effect-of-diosgenin-on-chondrocyte-metabolisms-in-osteoarthritis
#10
Jun Liu, Xiaole He, Ping Zhen, Hui Chen, Shenghu Zhou, Qi Tian, Rong Wang, Xusheng Li
To observe the effect of sirtuin type 1 (SIRT1) signaling pathway on chondrocyte metabolism and mitochondrial oxidative stress, to explore roles of SIRT1 signaling pathway and diosgenin (Dgn) in pathogenesis of osteoarthritis (OA).
 Methods: To establish C57BL/6 mouse (13.5-18.0 g) model of OA. The chondrocytes were randomly assigned into 4 groups: an OA group, a Dgn+OA group, a Dgn+Sirtinol+OA group, and a Sirtinol+OA group. Expressions of SIRT1, acetylation-regulated transcription factor 1 (Ac-FOXO1), and Bax were detected in the 4 groups by Western blot...
February 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28253664/membrane-acoustic-metamaterial-absorbers-with-magnetic-negative-stiffness
#11
Junjuan Zhao, Xianhui Li, Yueyue Wang, Wenjiang Wang, Bin Zhang, Xiaoling Gai
A membrane absorber usually requires a large back cavity to achieve low-frequency sound absorption. This paper describes the design of a membrane acoustic metamaterial absorber in which magnetic negative stiffness is employed to reduce the size of the back cavity. As a baseline for the present research, analysis of a typical membrane sound absorber based on an equivalent circuit model is presented first. Then, a theoretical model is established by introducing negative stiffness into a standard absorber. It is demonstrated that a small cavity with negative stiffness can achieve the acoustic impedance of a large cavity and that the absorption peak is shifted to lower frequencies...
February 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28252647/casc2c-as-an-unfavorable-prognosis-factor-interacts-with-mir-101-to-mediate-astrocytoma-tumorigenesis
#12
Changhong Liu, Yingnan Sun, Xiaoling She, Chaofeng Tu, Xiping Cheng, Lin Wang, Zhibin Yu, Peiyao Li, Qing Liu, Honghui Yang, Guiyuan Li, Minghua Wu
miR-101 has been suggested as a tumor suppressor, but the promoter methylation and loss of heterozygosity didn't contribute to its low expression in astrocytoma. We investigated the role of a new long non-coding RNA CASC2c binding with miR-101. High CASC2c was positively correlated with astrocytoma progression, and an unfavorable prognosis factor for patients. Knockdown CASC2c inhibited proliferation and tumorgenesis. Overexpression of CASC2c promotes the malignant characteristic of astrocytoma cells.CASC2c directly bound miR-101 and mediated pre-miR-101 processing into mature miR-101, and functions as a competitor of miR-101 target genes such as CPEB1...
March 2, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28242601/a-genetic-study-of-the-regeneration-capacity-of-embryonic-callus-from-the-maize-immature-embryo-culture
#13
Zhang Xiaoling, Long Yun, Ge Fei, Guan Zhongrong, Zhang Xiaoxiang, Wang Yanli, Shen Yaou, Pan Guangtang
We carried out a study of regeneration capacities of embryonic callus from maize immature embryo culture with 144 different inbred lines of natural groups from different countries, and found that the regeneration capacity was affected by three factors: environment, genotype and the interaction between the environment and genotype. We found that green embryonic callus rate (GCR), embryonic callus differentiating rate (CDR) and the plantlet number of embryonic callus regeneration (CPN) have significant positive correlations with each other, and they all have significant negative correlations with embryonic callus browning rate (CBR)...
February 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28241356/testosterone-treatment-and-cognitive-function-in-older-men-with-low-testosterone-and-age-associated-memory-impairment
#14
RANDOMIZED CONTROLLED TRIAL
Susan M Resnick, Alvin M Matsumoto, Alisa J Stephens-Shields, Susan S Ellenberg, Thomas M Gill, Sally A Shumaker, Debbie D Pleasants, Elizabeth Barrett-Connor, Shalender Bhasin, Jane A Cauley, David Cella, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, John T Farrar, Cora E Lewis, Mark E Molitch, Marco Pahor, Ronald S Swerdloff, Denise Cifelli, Stephen Anton, Shehzad Basaria, Susan J Diem, Christina Wang, Xiaoling Hou, Peter J Snyder
Importance: Most cognitive functions decline with age. Prior studies suggest that testosterone treatment may improve these functions. Objective: To determine if testosterone treatment compared with placebo is associated with improved verbal memory and other cognitive functions in older men with low testosterone and age-associated memory impairment (AAMI). Design, Setting, and Participants: The Testosterone Trials (TTrials) were 7 trials to assess the efficacy of testosterone treatment in older men with low testosterone levels...
February 21, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28241355/testosterone-treatment-and-coronary-artery-plaque-volume-in-older-men-with-low-testosterone
#15
RANDOMIZED CONTROLLED TRIAL
Matthew J Budoff, Susan S Ellenberg, Cora E Lewis, Emile R Mohler, Nanette K Wenger, Shalender Bhasin, Elizabeth Barrett-Connor, Ronald S Swerdloff, Alisa Stephens-Shields, Jane A Cauley, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, Thomas M Gill, Alvin M Matsumoto, Mark E Molitch, Rine Nakanishi, Negin Nezarat, Suguru Matsumoto, Xiaoling Hou, Shehzad Basaria, Susan J Diem, Christina Wang, Denise Cifelli, Peter J Snyder
Importance: Recent studies have yielded conflicting results as to whether testosterone treatment increases cardiovascular risk. Objective: To test the hypothesis that testosterone treatment of older men with low testosterone slows progression of noncalcified coronary artery plaque volume. Design, Setting, and Participants: Double-blinded, placebo-controlled trial at 9 academic medical centers in the United States. The participants were 170 of 788 men aged 65 years or older with an average of 2 serum testosterone levels lower than 275 ng/dL (82 men assigned to placebo, 88 to testosterone) and symptoms suggestive of hypogonadism who were enrolled in the Testosterone Trials between June 24, 2010, and June 9, 2014...
February 21, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28241237/association-of-testosterone-levels-with-anemia-in-older-men-a-controlled-clinical-trial
#16
Cindy N Roy, Peter J Snyder, Alisa J Stephens-Shields, Andrew S Artz, Shalender Bhasin, Harvey J Cohen, John T Farrar, Thomas M Gill, Bret Zeldow, David Cella, Elizabeth Barrett-Connor, Jane A Cauley, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, Cora E Lewis, Alvin M Matsumoto, Mark E Molitch, Marco Pahor, Ronald S Swerdloff, Denise Cifelli, Xiaoling Hou, Susan M Resnick, Jeremy D Walston, Stephen Anton, Shehzad Basaria, Susan J Diem, Christina Wang, Stanley L Schrier, Susan S Ellenberg
Importance: In one-third of older men with anemia, no recognized cause can be found. Objective: To determine if testosterone treatment of men 65 years or older with unequivocally low testosterone levels and unexplained anemia would increase their hemoglobin concentration. Design, Setting, and Participants: A double-blinded, placebo-controlled trial with treatment allocation by minimization using 788 men 65 years or older who have average testosterone levels of less than 275 ng/dL...
February 21, 2017: JAMA Internal Medicine
https://www.readbyqxmd.com/read/28241231/effect-of-testosterone-treatment-on-volumetric-bone-density-and-strength-in-older-men-with-low-testosterone-a-controlled-clinical-trial
#17
Peter J Snyder, David L Kopperdahl, Alisa J Stephens-Shields, Susan S Ellenberg, Jane A Cauley, Kristine E Ensrud, Cora E Lewis, Elizabeth Barrett-Connor, Ann V Schwartz, David C Lee, Shalender Bhasin, Glenn R Cunningham, Thomas M Gill, Alvin M Matsumoto, Ronald S Swerdloff, Shehzad Basaria, Susan J Diem, Christina Wang, Xiaoling Hou, Denise Cifelli, Darlene Dougar, Bret Zeldow, Douglas C Bauer, Tony M Keaveny
Importance: As men age, they experience decreased serum testosterone concentrations, decreased bone mineral density (BMD), and increased risk of fracture. Objective: To determine whether testosterone treatment of older men with low testosterone increases volumetric BMD (vBMD) and estimated bone strength. Design, Setting, and Participants: Placebo-controlled, double-blind trial with treatment allocation by minimization at 9 US academic medical centers of men 65 years or older with 2 testosterone concentrations averaging less than 275 ng/L participating in the Testosterone Trials from December 2011 to June 2014...
February 21, 2017: JAMA Internal Medicine
https://www.readbyqxmd.com/read/28241078/genetic-evidence-from-mitochondrial-dna-corroborates-the-origin-of-tibetan-chickens
#18
Long Zhang, Pu Zhang, Qingqing Li, Uma Gaur, Yiping Liu, Qing Zhu, Xiaoling Zhao, Yan Wang, Huadong Yin, Yaodong Hu, Aiping Liu, Diyan Li
Chicken is the most common poultry species and is important to human societies. Tibetan chicken (Gallus gallus domesticus) is a breed endemic to China that is distributed mainly on the Qinghai-Tibet Plateau. However, its origin has not been well characterized. In the present study, we sequenced partial mitochondrial DNA (mtDNA) control region of 239 and 283 samples from Tibetan and Sichuan indigenous chickens, respectively. Incorporating 1091 published sequences, we constructed the matrilineal genealogy of Tibetan chickens to further document their domestication history...
2017: PloS One
https://www.readbyqxmd.com/read/28239907/effects-of-oral-%C3%AE-lipoic-acid-administration-on-body-weight-in-overweight-or-obese-subjects-a-crossover-randomized-double-blind-placebo-controlled-trial
#19
Nong Li, Weili Yan, Xiaojuan Hu, Yongdi Huang, Fugang Wang, Weiguo Zhang, Qian Wang, Xiaoling Wang, Kehong Sun
OBJECTIVE: Alpha-lipoic acid (ALA) has shown beneficial properties on diabetes and obesity. The aim of this study was to examine the effects of oral ALA on body weight in subjects with overweight or obese. DESIGN: Single-centre, randomized, double-blind, crossover controlled study. PARTICIPANTS: A total of 166 subjects of Chinese Han ethnicity with a BMI ≥25 kg/m(2) were screened and 103 subjects fulfilled the study requirements, in terms of informed consent and participation to the study...
January 12, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28239355/modification-of-flight-and-locomotion-performances-respiratory-metabolism-and-transcriptome-expression-in-the-lady-beetle-harmonia-axyridis-through-sublethal-pesticide-exposure
#20
Da Xiao, Xiaoling Tan, Wenjuan Wang, Fan Zhang, Nicolas Desneux, Su Wang
Biological control is usually used in combination with chemical control for practical agricultural applications. Thus, the influence of insecticides on the natural predators used for biological control should be investigated for integrated pest management. The ladybird Harmonia axyridis is an effective predator on aphids and coccids. Beta-cypermethrin is a broad-spectrum insecticide used worldwide for controlling insect pests. H. axyridis is becoming increasingly threatened by this insecticide. Here, we investigated the effect of a sublethal dose of beta-cypermethrin on flight, locomotion, respiration, and detoxification system of H...
2017: Frontiers in Physiology
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