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Michal Wen Sheue Ong, Rashim Salota, Tracy Reeman, Lapsley Marta, Lydia Jones
The most commonly used techniques to measure vitamin D are automated immunoassays which are known to be affected by interferences, especially from immunoglobulins present in the patient's serum. We present a case of a patient with myeloma in whom interference with the vitamin D assay was identified. An eighty-three year old female, known to have IgG myeloma was found to have a high concentration of 25-OH vitamin D on a routine test without any signs of vitamin D toxicity. She was not taking vitamin D supplements or any other multivitamin preparation and had minimal sun exposure...
January 1, 2017: Annals of Clinical Biochemistry
Tapan Kumar Singh Nayak, Neeraja P Alamuru-Yellapragada, Kishore V L Parsa
Post translational modifications, ubiquitination and its reversal by deubiquitination play an important role in regulating innate immune system. USP12 is a poorly studied deubiquitinase reported to regulate T-cell receptor signaling however the functional role of USP12 in macrophages, the principal architects of inflammation, is unknown. Thus, in this study we probed the involvement of USP12 in macrophage mediated inflammatory responses using bacterial endotoxin, LPS, as the model system. Here, we observed that the expression of USP12 was altered in time dependent manner in LPS stimulated RAW 264...
January 4, 2017: Biochemical and Biophysical Research Communications
Alon Eisen, Marc P Bonaca, Petr Jarolim, Benjamin M Scirica, Harvey D White, Michal Tendera, Mikael Dellborg, Jose C Nicolau, Joao Morais, Keith A A Fox, Erin A Bohula, Sabina A Murphy, Eugene Braunwald, David A Morrow
BACKGROUND: Cardiac troponin I, measured with a high-sensitivity assay (hs-TnI), is well-established for risk prediction in acute coronary syndromes. However, its prognostic role in stable atherosclerotic disease, particularly for future myocardial infarction (MI), is less well defined. METHODS: We measured hs-TnI (Abbott ARCHITECT) in 15833 patients with prior MI, ischemic stroke, or peripheral arterial disease from the placebo-controlled Thrombin Receptor Antagonist in Secondary Prevention of Atherothrombotic Ischemic Events (TRA 2°P)-Thrombolysis in Myocardial Infarction (TIMI) 50 trial of the platelet inhibitor vorapaxar, excluding patients with recent MI (<30 days)...
January 2017: Clinical Chemistry
Mridula Madiyal, Siddharth Sagar, Shashidhar Vishwanath, Barnini Banerjee, Vandana Kalwaje Eshwara, Kiran Chawla
INTRODUCTION: Antibodies to Hepatitis B surface Antigen (Anti-HBs) levels are measured as markers for immune response to vaccination and in decision making for post-exposure prophylaxis against Hepatitis-B. Several immunoassay formats are used to measure Anti-HBs, thus carrying the possibility of variation in measured levels between different assays. This study compares the performance of Chemiluminescence Immunoassay (CLIA) against Enzyme-linked Immunosorbent Assay (ELISA) in measuring Anti-HBs titer by looking into concordance between the two test reports...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
Prabhjot Singh, Surinder K Brar, Manish Bajaj, Nikesh Narang, Venus S Mithu, Om P Katare, Nishima Wangoo, Rohit K Sharma
In the pursuit for design of novel bio inspired materials, aromatic α-amino acids (phenylalanine, tyrosine, tryptophan and histidine) have been investigated for the generation of well-ordered self-assembled architects such as fibrils, rods, ribbons and twisted nanosheets in varying solvent systems. These nano/micro scaled architects were thoroughly characterized using FE-SEM, confocal microscopy, optical microscopy, (1)H NMR, FTIR, XRD and TGA. These self-assembled architects were histologically stained with Congo red and thioflavin T dyes for investigation of amyloid morphology which revealed that the deposited state of ordered assemblies exhibit specific characteristic of amyloid deposits...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
M Mayer-Pinto, E L Johnston, A B Bugnot, T M Glasby, L Airoldi, A Mitchell, K A Dafforn
Urbanisation in terrestrial systems has driven architects, planners, ecologists and engineers to collaborate on the design and creation of more sustainable structures. Examples include the development of 'green infrastructure' and the introduction of wildlife corridors that mitigate urban stressors and provide positive ecological outcomes. In contrast, efforts to minimise the impacts of urban developments in marine environments have been far more restricted in their extent and scope, and have often overlooked the ecological role of the built environment as potential habitat...
December 23, 2016: Journal of Environmental Management
Steven De Keukeleire, Stefanie Desmet, Katrien Lagrou, Julie Oosterlynck, Manon Verhulst, Jessica Van Besien, Veroniek Saegeman, Marijke Reynders
The performance of Elecsys Syphilis was compared to Architect Syphilis TP and Reformulated Architect Syphilis TP. The overall sensitivity and specificity were 98.4% and 99.5%, 97.7% and 97.1%, and 99.2% and 99.7% respectively. The assays are comparable and considered adequate for syphilis screening.
December 9, 2016: Diagnostic Microbiology and Infectious Disease
Ergenekon Karagoz, Mehmet Burak Selek, Alpaslan Tanoglu, Mustafa Hatipoglu, Asim Ulçay, Vedat Turhan
Quantitative hepatitis B surface antigen (HBsAg) is a valuable tool in hepatitis B virus (HBV) disease diagnosis and management for evaluating the effectiveness of antiviral therapy. The aim of the current research was to compare the performances of the Elecsys HBsAg II and Abbott Architect HBsAg assays in chronic hepatitis B (CHB) patients. Between May 2014 and December 2014, 72 CHBs were tested using Abbott Architect HBsAg QT and Roche Elecsys HBsAg II assay. After transformation to log (10) IU/mL, the results of the two assays were compared using the interclass correlation test, the Pearson correlation test and Bland-Altman analyses...
1, 2016: Le Infezioni in Medicina
A Aguinaga, A Navascués, I Polo, C Ezpeleta
OBJECTIVE: The aim of the study is to compare two confirmatory tests for HIV-1/2 infection. METHODS: A prospective study was carried out between 01/01/2015 and 12/31/2015. Serum samples with repeatedly positive results in the Antibody-Antigen-HIV-1/2 (Architect, Abbott) screening assay were included. The serum samples corresponding to new diagnosed cases were selected and were used to compare the two confirmatory assays: Geenius™ HIV-1/2 (Bio-Rad) and INNO-LIA™ HIV-1/2 score line-immunoassay (Innogene-tics®)...
December 23, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
Yolanda Keys, Susan R Silverman, Jennie Evans
The purpose of this study was to collect the perceptions of design professionals and clinicians regarding design process success strategies and elements of interprofessional engagement and communication during healthcare design and construction projects. Additional objectives were to gather best practices to maximize clinician engagement and provide tools and techniques to improve interdisciplinary collaboration for future projects. Strategies are needed to enhance the design and construction process and create interactions that benefit not only the project but the individuals working to see its completion...
January 1, 2016: HERD
Catherine R Wasser, Joachim Herz
Over half a century ago, D.S. Falconer first reported a mouse with a reeling gate (1). Four-decades later, the Reln gene was isolated and identified as the cause of the reeler phenotype (reviewed in (2)). Initial studies found that loss of Reelin, a large, secreted glycoprotein encoded by the Reln gene, results in abnormal neuronal layering throughout several regions of the brain (reviewed in (2-4)). In the years since, the known functions of Reelin signaling in the brain have expanded to include multiple post-developmental neuromodulatory roles, revealing an ever-increasing body of evidence to suggest that Reelin signaling is a critical player in the modulation of synaptic function (reviewed in (5))...
December 19, 2016: Journal of Biological Chemistry
Noboru Shinkai, Shigeru Kusumoto, Shuko Murakami, Shintaro Ogawa, Masaki Ri, Tekeshi Matsui, Akihiro Tamori, Hidenori Toyoda, Takashi Ishida, Shinsuke Iida, Yasuhito Tanaka
BACKGROUND & AIMS: Occult hepatitis B virus (HBV) infection should be evaluated before systemic chemotherapy to prevent HBV reactivation-related hepatitis. We investigated HBV reactivation using high sensitivity HB surface antigen (HBsAg) chemiluminescent enzyme immunoassay (HBsAg-HQ) and ultra-high sensitive HBsAg assay employing a semi-automated immune complex transfer chemiluminescence enzyme technique (ICT-CLEIA). METHODS: Of 120 HBV-resolved patients with hematological malignancy receiving systemic chemotherapy from 2012 to 2015 in our hospital, 13 patients had HBV DNA reactivation (in 12/13 patients HBV DNA became quantifiable) according to HBV DNA monitoring...
December 19, 2016: Liver International: Official Journal of the International Association for the Study of the Liver
Stijn Raven, Jeannine Hautvast, Jim van Steenbergen, Reinier Akkermans, Cas Weykamp, Francis Smits, Christian Hoebe, Ann Vossen
BACKGROUND: Post-vaccination testing after hepatitis B vaccination is indispensable to evaluate long-term immunological protection. Using a threshold level of antibodies against hepatitis B surface antigen (anti-HBs) to define serological protection, implies reproducible and valid measurements of different diagnostic assays. OBJECTIVES: In this study we assess the performance of currently used anti-HBs assays. STUDY DESIGN: In 2013, 45 laboratories participated in an external quality assessment program using pooled anti-HBs serum samples around the cutoff values 10IU/l and 100IU/l...
December 7, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Ninad T Trifale, Eric A Nauman, Kazuaki Yazawa
Controlling the unit-cell topology of microlattice structures can enable the customization of effective anisotropic material properties. A wide range of properties can be obtained by varying connectivity within the unit cell, which then can be further used to optimize structures specific to applications. A methodology for a systematic generation of microlattice structures is presented that focuses on controlling discrete topology instead of average porosity (as is done in conventional porous media). An algorithm is developed to create valid lattice structures without redundancies from a given set of template nodes...
December 28, 2016: ACS Applied Materials & Interfaces
Praveen K Chinthakindi, Tricia Naicker, Niranjan Thota, Thavendran Govender, Hendrik G Kruger, Per I Arvidsson
Synthesis and evaluation of structural analogues and isosteres represent a cornerstone methodology among the practitioners of medicinal- and agrochemistry. The sulfonamide functional group represents one of the most important amide isosteres in contemporary drug design, with some 500 compounds that has overcome both the pharmacological and regulatory hurdles that precede studies in humans. We note that mono aza-analogues of sulfonamides, i.e. sulfonimidamides, are rapidly gaining popularity as a novel functional group among molecular architects involved in the design of biologically active compounds for both pharmaceutical- and agrochemical applications...
December 13, 2016: Angewandte Chemie
C F Béguin
To study the noteworthy nest building behavior of the nymph of the Brazilian Rainforest cicada Guyalna chlorogena (Walker) during the last year of its underground life, we monitored a large number of edifices, consisting of a vertical well (up to 1 m deep) with a turret (20 to 40 cm tall) on top, and we also performed experiments. We have shown that the buildings are occupied by a single nymph, male or female, which increases the height of its turret each night by about 3 cm, during a short active growing phase...
December 12, 2016: Neotropical Entomology
Colleen K McIlvennan, Daniel D Matlock
No abstract text is available yet for this article.
December 9, 2016: Journal of Cardiac Failure
Xue Qing David Wang, Josée Dostie
There are many ways in which cells may not adequately behave or respond to their environment, and the molecular mechanisms leading to these defects are as diverse as they are many. In this review, we report on how spatial chromatin organization contributes to the proper expression of genes, relating how CTCF-one of its main architects-contributes to gene regulation. We also touch on the emerging role of long noncoding RNAs in shaping chromatin organization and activity. The HOX gene clusters have been used as paradigm in the study of various biological pathways, and the overview we provide gives emphasis to what research on these loci has revealed about chromatin architecture and its regulation in the control of gene expression...
December 8, 2016: Current Opinion in Genetics & Development
Michael Wilhelmsen, Ib J Christensen, Louise Rasmussen, Lars N Jørgensen, Mogens R Madsen, Jesper Vilandt, Thore Hillig, Michael Klaerke, Knud T Nielsen, Søren Laurberg, Nils Brünner, Susan Gawel, Xiaoqing Yang, Gerard Davis, Anne Meike Heijboer, Frans Martens, Hans J Nielsen
Serological biomarkers may be an option for early detection of colorectal cancer (CRC). The present study assessed 8 cancer-associated protein biomarkers in plasma from subjects undergoing first time ever colonoscopy due to symptoms attributable to colorectal neoplasia. Plasma AFP, CA19-9, CEA, hs-CRP, CyFra21-1, Ferritin, Galectin-3, and TIMP-1 were determined in EDTA-plasma using the Abbott ARCHITECT® automated immunoassay platform. Primary endpoints were detection of: 1: CRC and high risk adenoma and 2: CRC...
December 9, 2016: International Journal of Cancer. Journal International du Cancer
Yu-Cheng Chen, Tzu-Ping Lin, Chien-Ting Lin
Motivated by the increasing thermal load in urban environment, this work established Urban Climatic map (UCmap) focusing on thermal environment issues based on urban development factors, e.g., land cover and building characteristics, representing thermal load of human body and ventilation path in the urban structures. In the established process of UCmap in this work, Tainan city, which is a highly developed city in southern Taiwan, is selected as the research area. A 50-m resolution grid is used to capture urban development factors and the climate data based on 1 year of mobile and fix-point measurements, from which the thermal load and the wind environment map are constructed...
December 3, 2016: International Journal of Biometeorology
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