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https://www.readbyqxmd.com/read/28214872/higher-numbers-of-t-bet-tumor-infiltrating-lymphocytes-associate-with-better-survival-in-human-epithelial-ovarian-cancer
#1
Yun Xu, Lujun Chen, Bin Xu, Yuqi Xiong, Min Yang, Xiaohui Rui, Liangrong Shi, Changping Wu, Jingting Jiang, Binfeng Lu
BACKGROUND/AIMS: T-bet, a member of the T-box family of transcription factors, is a key marker of type I immune response within the tumor microenvironment, and has been previously reported by us to serve as an important prognostic indicator for human gastric cancer patients and a potential biomarker for immunotherapy. In the present study, we aimed to assess the clinical significance and prognostic value of T-bet+ tumor-infiltrating lymphocytes in human epithelial ovarian cancer. METHODS: The immunohistochemistry was used to analyze the infiltration density of T-bet+ lymphoid cells in human epithelial ovarian cancer tissues, and the flow cytometry analysis was used to further analyze the presence of T-bet+ tumor-infiltrating lymphocytes subgroups in cancer tissues...
January 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214357/nesting-habits-influence-population-genetic-structure-of-a-bee-living-in-anthropogenic-disturbance
#2
J L Vickruck, M H Richards
While most organisms are negatively affected by anthropogenic disturbance, a few species thrive in landscapes altered by humans. Typically, native bees are negatively impacted by anthropogenic environmental change, including habitat alteration and climate change. Here we investigate the population structure of the eastern carpenter bee Xylocopa virginica, a generalist pollinator with a broad geographic range spanning eastern North America. Eastern carpenter bees now nest almost exclusively in human made wooden structures, linking their geographic distribution and population structure to human activities and disturbance...
February 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28214275/detection-of-pneumocystis-jirovecii-by-nested-pcr-in-hiv-negative-patients-with-pulmonary-disease
#3
Cristina Rodrigues Santos, Ângela M de Assis, Edson A Luz, Luzia Lyra, Ivan F Toro, José Claudio C Seabra, Dira H Daldin, Tathiane U Marcalto, Marcos T Galasso, Ronaldo F Macedo, Angélica Z Schreiber, Francisco H Aoki
BACKGROUND: Nested PCR can be used to determine the status of Pneumocystis jirovecii infection in other lung diseases. AIMS: This study sought to detect a target DNA fragment (mitochondrial large subunit rRNA or mtL SUrRNA) of P. jirovecii in patients with lung disease who underwent bronchoscopy with collection of bronchoalveolar lavage (BAL). METHODS: The results from toluidine blue staining were compared with those obtained using molecular methods that included an "in house" DNA extraction procedure, PCR and nested PCR...
February 14, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28213870/molecular-characterization-of-bovine-leukemia-virus-from-moldovan-dairy-cattle
#4
Aneta Pluta, Marzena Rola-Łuszczak, Piotr Kubiś, Svetlana Balov, Roman Moskalik, Bhudipa Choudhury, Jacek Kuźmak
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cattle from three farms in three regions of Moldova were screened by ELISA and nested PCR. Of these cattle, 14 which were PCR positive, and these were molecularly characterized based on the nucleotide sequence of the env gene and the deduced amino acid sequence of the encoded gp51 protein...
February 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28213475/wheat-landrace-genome-diversity
#5
Luzie U Wingen, Claire West, Michelle Leverington-Waite, Sarah Collier, Simon Orford, Richard Goram, Cai-Yun Yang, Julie King, Alexandra M Allen, Amanda Burridge, Keith J Edwards, Simon Griffiths
Understanding the genomic complexity of bread wheat (Triticum aestivum L.) is a cornerstone in the quest to unravel the processes of domestication and the following adaptation of domesticated wheat to a wide variety of environments across the globe. Additionally, it is of importance for future improvement of the crop, particularly in the light of climate change. Focussing on the adaptation after domestication, a nested association mapping (NAM) panel of 60 segregating bi-parental populations were developed mainly involving landrace accessions from the core set of the Watkins hexaploid wheat collection optimized for genetic diversity (WINGEN et al...
February 17, 2017: Genetics
https://www.readbyqxmd.com/read/28213300/experiences-of-stigma-and-discrimination-faced-by-family-caregivers-of-people-with-schizophrenia-in-india
#6
Mirja Koschorke, R Padmavati, Shuba Kumar, Alex Cohen, Helen A Weiss, Sudipto Chatterjee, Jesina Pereira, Smita Naik, Sujit John, Hamid Dabholkar, Madhumitha Balaji, Animish Chavan, Mathew Varghese, R Thara, Vikram Patel, Graham Thornicroft
Stigma associated with schizophrenia significantly affects family caregivers, yet few studies have examined the nature and determinants of family stigma and its relationship to their knowledge about the condition. This paper describes the experiences and determinants of stigma reported by the primary caregivers of people living with schizophrenia (PLS) in India. The study used mixed methods and was nested in a randomised controlled trial of community care for people with schizophrenia. Between November 2009 and October 2010, data on caregiver stigma and functional outcomes were collected from a sample of 282 PLS-caregiver dyads...
February 1, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28212874/multilocus-phylogeny-of-alligator-lizards-elgaria-anguidae-testing-mtdna-introgression-as-the-source-of-discordant-molecular-phylogenetic-hypotheses
#7
Dean H Leavitt, Angela B Marion, Bradford D Hollingsworth, Tod W Reeder
The increased availability of nuclear DNA sequence data has led to a better appreciation of the role and frequency of introgressive hybridization and subsequent mitochondrial capture in misleading phylogenetic hypotheses based on mtDNA sequence data alone. Relationships among members of the alligator lizard genus Elgaria have been addressed with morphology, allozyme and mtDNA sequence data with discordant results. In this study, we use seven nuclear loci (total of 5.9 kb) and ∼3 kb of mtDNA to infer the phylogenetic relationships among Elgaria species and test whether the discordance among previous phylogenetic hypotheses is due to introgression and mtDNA capture...
February 14, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28212672/cytokeratin7-and-cytokeratin19-expression-in-high-grade-cervical-intraepithelial-neoplasm-and-squamous-cell-carcinoma-and-their-possible-association-in-cervical-carcinogenesis
#8
Hojung Lee, Hyekyung Lee, Yong Kyun Cho
BACKGROUND: High risk human papillomavirus (HR HPV) infects cells at the squamocolumnar junction (SCJ) of the cervix, causing cancer. Cytokeratin (CK)7 is an SCJ marker, and stains cervical neoplasia. CK19 is a binding partner of CK7 and expressed in cervical cancer. Despite this possible association between CK7/CK19 and cervical cancer, not much is known about the mechanism of CK7/CK19 involvement in HR HPV-mediated cervical carcinogenesis. METHODS: We analyzed the expression pattern of CK7, CK19, and p16 by using immunohistochemistry and HPV infection by in situ hybridization in 25 cases of high grade cervical intraepithelial neoplasia (CIN3) and in 30 cases of squamous cell carcinoma (SCC)...
February 17, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/28212298/prevalence-and-clinical-impact-of-human-pegivirus-1-infection-in-hiv-1-infected-individuals-in-yunnan-china
#9
Zhijiang Miao, Li Gao, Yindi Song, Ming Yang, Mi Zhang, Jincheng Lou, Yue Zhao, Xicheng Wang, Yue Feng, Xingqi Dong, Xueshan Xia
Human Pegivirus-1 (HPgV-1) may have a beneficial impact on disease progression in human immunodeficiency virus-1 (HIV-1) infection. However, analysis of the genotypic diversity of HPgV-1 and its relevance to the progression of HIV-1 disease remains limited. A total of 1062 HIV-1-infected individuals were recruited in all sixteen prefectures of Yunnan province, China. The reverse transcription nested polymerase chain reaction (RT-nPCR), phylogenetic analyses, and clinical data analyses were used to detect HPgV-1 infection, determine genotype, and analyze HPgV-1 genotype impact on HIV-1 disease progression...
February 15, 2017: Viruses
https://www.readbyqxmd.com/read/28209552/detection-of-giardia-duodenalis-assemblage-e-infections-at-the-tibetan-plateau-area-yaks-are-suitable-hosts
#10
Wang Guang-Hua, Wang Ge-Ping, Li Xiu-Ping, Ma Li-Qing, Gabriele Karanis, Eleni Christodoulou-Vafeiadou, Panagiotis Karanis
The prevalence of Giardia duodenalis (G. duodenalis) assemblages in yaks is poorly known. The present study examined 297 fecal samples from weaned yak, 4 to 7 months of age, from 3 different farms, in Tibetan Plateau Area (TPA) of the Qinghai Province in Western China. The prevalence of infection was determined by light and immunofluorescence microscopy, and nested-PCR. PCR was performed for the small subunit ribosomal RNA (SSU) amplified 16 positive for G. duodenalis products. The prevalence of Giardia species was 5...
February 13, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28208132/an-outbreak-of-acute-hepatitis-caused-by-genotype-ib-hepatitis-a-viruses-contaminating-the-water-supply-in-thailand
#11
Kriangsak Ruchusatsawat, Jongkonnee Wongpiyabovorn, Chonthicha Kawidam, Laddawan Thiemsing, Somchai Sangkitporn, Sayaka Yoshizaki, Masashi Tatsumi, Naokazu Takeda, Koji Ishii
BACKGROUND: In 2000, an outbreak of acute hepatitis A was reported in a province adjacent to Bangkok, Thailand. AIMS: To investigate the cause of the 2000 hepatitis A outbreaks in Thailand using molecular epidemiological analysis. METHODS: Serum and stool specimens were collected from patients who were clinically diagnosed with acute viral hepatitis. Water samples from drinking water and deep-drilled wells were also collected. These specimens were subjected to polymerase chain reaction (PCR) amplification and sequencing of the VP1/2A region of the hepatitis A virus (HAV) genome...
February 17, 2017: Intervirology
https://www.readbyqxmd.com/read/28208127/prevalence-of-occult-hepatitis-c-virus-infection-among-blood-donors-in-jiangsu-china
#12
Hong Lin, Xian Chen, Shaowen Zhu, Ping Mao, Shanshan Zhu, Yanchun Liu, Chengyin Huang, Jun Sun, Jin Zhu
OBJECTIVE: In 2010, only 1 donor blood sample was found to be anti-hepatitis C virus (HCV) negative and HCV RNA positive, as detected by nucleic acid testing. In occult HCV infection (OCI), HCV RNA is found in peripheral blood mononuclear cells (PBMCs). We investigated the prevalence of OCI among blood donors. METHODS: We collected 513 samples from 334 eligible and 179 deferred donors, including 55 anti-HCV-positive, 113 alanine aminotransferase (ALT)-elevated, and 11 hepatitis B virus surface antigen (HBsAg)-positive blood donors...
February 17, 2017: Intervirology
https://www.readbyqxmd.com/read/28208013/sex-differences-in-autism-like-behavioral-phenotypes-and-postsynaptic-receptors-expression-in-the-prefrontal-cortex-of-tert-transgenic-mice
#13
Ki Chan Kim, Kyu Suk Cho, Sung Min Yang, Edson Luck Gonzales, Schley Valencia, Pyeong Hwa Eun, Chang Soon Choi, Darine Froy Mabunga, Ji-Woon Kim, Judy Kyoungju Noh, Hee Jin Kim, Se Jin Jeon, Seol-Heui Han, Geon Ho Bahn, Chan Young Shin
Autism spectrum disorder (ASD) remains unexplained and untreated despite the high attention of research in recent years. Aside from its various characteristics is the baffling male preponderance over the female population. Using a validated animal model of ASD which is the telomerase reverse transcriptase overexpressing mice (TERT-tg), we conducted ASD-related behavioral assessments and protein expression experiments to mark the difference between male and females of this animal model. After statistically analyzing the results, we found significant effects of TERT overexpression in sociability, social novelty preference, anxiety, nest building, and electroseizure threshold in the males but not their female littermates...
February 17, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28207957/phylogenetic-relatedness-explains-highly-interconnected-and-nested-symbiotic-networks-of-woody-plants-and-arbuscular-mycorrhizal-fungi-in-a-chinese-subtropical-forest
#14
Liang Chen, Yong Zheng, Cheng Gao, Xiang-Cheng Mi, Ke-Ping Ma, Tesfaye Wubet, Liang-Dong Guo
Elucidating symbiotic relationships between arbuscular mycorrhizal fungi (AMF) and plants contributes to a better understanding of their reciprocally dependent co-existence and community assembly. However, the main drivers of plant and AMF community assembly remain unclear. In this study, we examined AMF communities from 166 root samples of 17 woody plant species from 10 quadrats in a Chinese subtropical forest using 454 pyrosequencing of 18S rRNA gene to describe symbiotic AMF-plant association. Our results show the woody plant-AMF networks to be highly interconnected and nested, but in anti-modular and anti-specialized manners...
February 16, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28207749/determinants-of-first-trimester-attendance-at-antenatal-care-clinics-in-the-amazon-region-of-peru-a-case-control-study
#15
Nora Moore, Brittany Blouin, Hugo Razuri, Martin Casapia, Theresa W Gyorkos
OBJECTIVE: To identify determinants which influence the timing of the first antenatal care (ANC) visit in pregnant women. DESIGN: Retrospective matched nested case-control study. SETTING: Two health centres, Belén and 6 de Octubre, in the Peruvian Amazon. POPULATION: All pregnant women who had attended ANC during the years 2010, 2011, and 2012. METHODS: All cases (819 women initiating ANC in their first trimester) were selected from ANC registries from 2010 to 2012...
2017: PloS One
https://www.readbyqxmd.com/read/28206898/fluoride-varnish-applications-in-preschoolers-and-dental-fluorosis-in-permanent-incisors-results-of-a-nested-cohort-study-within-a-clinical-trial
#16
Ana Paula Pires Dos Santos, Marcella Cristina Bordallo Malta, Mirian de Waele Souchois de Marsillac, Branca Heloisa de Oliveira
PURPOSE: To compare the prevalence and severity of fluorosis in the permanent maxillary incisors of children who had participated in a two-year randomized placebo-controlled clinical trial on fluoride varnish application in the primary dentition and to assess children's esthetic perception of their teeth. METHODS: Parents of 200 one- to four-year-old children who had received biannual applications of fluoride or placebo varnish were contacted four years after the end of the trial...
October 15, 2016: Pediatric Dentistry
https://www.readbyqxmd.com/read/28205612/dna-analysis-of-a-30-000-year-old-urocitellus-glacialis-from-northeastern-siberia-reveals-phylogenetic-relationships-between-ancient-and-present-day-arctic-ground-squirrels
#17
Marina Faerman, Gila Kahila Bar-Gal, Elisabetta Boaretto, Gennady G Boeskorov, Nikolai E Dokuchaev, Oleg A Ermakov, Fedor N Golenishchev, Stanislav V Gubin, Eugenia Mintz, Evgeniy Simonov, Vadim L Surin, Sergei V Titov, Oksana G Zanina, Nikolai A Formozov
In contrast to the abundant fossil record of arctic ground squirrels, Urocitellus parryii, from eastern Beringia, only a limited number of fossils is known from its western part. In 1946, unnamed GULAG prisoners discovered a nest with three mummified carcasses of arctic ground squirrels in the permafrost sediments of the El'ga river, Yakutia, Russia, that were later attributed to a new species, Citellus (Urocitellus) glacialis Vinogr. To verify this assignment and to explore phylogenetic relationships between ancient and present-day arctic ground squirrels, we performed (14)C dating and ancient DNA analyses of one of the El'ga mummies and four contemporaneous fossils from Duvanny Yar, northeastern Yakutia...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205047/pre-diagnosis-insulin-like-growth-factor-i-and-risk-of-epithelial-invasive-ovarian-cancer-by-histological-subtypes-a-collaborative-re-analysis-from-the-ovarian-cancer-cohort-consortium
#18
Jennifer Ose, Helena Schock, Elizabeth M Poole, Matti Lehtinen, Kala Visvanathan, Kathy Helzlsouer, Julie E Buring, I-Min Lee, Anne Tjønneland, Marie-Christine Boutron-Ruault, Antonia Trichopoulou, Amalia Mattiello, N Charlotte Onland-Moret, Elisabete Weiderpass, María-José Sánchez, Annika Idahl, Ruth C Travis, Sabina Rinaldi, Melissa A Merritt, Nicolas Wentzensen, Shelley S Tworoger, Rudolf Kaaks, Renée T Fortner
PURPOSE: Biologic evidence suggests that the Insulin-like growth factor (IGF)-family may be involved in the etiology of epithelial invasive ovarian cancer (EOC). However, prospective studies investigating the role of IGF-I in ovarian carcinogenesis have yielded conflicting results. METHODS: We pooled and harmonized data from 6 case-control studies nested within the Ovarian Cancer Cohort Consortium to investigate the association between pre-diagnosis IGF-I concentrations and subsequent risk of EOC...
February 16, 2017: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/28202810/how-numbers-of-nesting-sea-turtles-can-be-overestimated-by-nearly-a-factor-of-two
#19
Nicole Esteban, Jeanne A Mortimer, Graeme C Hays
Estimating the absolute number of individuals in populations and their fecundity is central to understanding the ecosystem role of species and their population dynamics as well as allowing informed conservation management for endangered species. Estimates of abundance and fecundity are often difficult to obtain for rare or cryptic species. Yet, in addition, here we show for a charismatic group, sea turtles, that are neither cryptic nor rare and whose nesting is easy to observe, that the traditional approach of direct observations of nesting has likely led to a gross overestimation of the number of individuals in populations and underestimation of their fecundity...
February 22, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28202653/argentine-ants-linepithema-humile-use-adaptable-transportation-networks-to-track-changes-in-resource-quality
#20
Tanya Latty, Michael J Holmes, James C Makinson, Madeleine Beekman
Transportation networks play a crucial role in human and animal societies. For a transportation network to be efficient, it must have adequate capacity to meet traffic demand. Network design becomes increasingly difficult in situations where traffic demand can change unexpectedly. In humans, network design is often constrained by path dependency because it is difficult to move a road once it is built. A similar issue theoretically faces pheromone-trail-laying social insects; once a trail has been laid, positive feedback makes re-routing difficult because new trails cannot compete with continually reinforced pre-existing trails...
February 15, 2017: Journal of Experimental Biology
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