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https://www.readbyqxmd.com/read/28044933/should-arterial-embolization-in-recurrent-spontaneous-hemophilic-hemarthroses-refractory-to-intensive-prophylaxis-be-the-first-invasive-resort
#1
E Carlos Rodriguez-Merchan
This paper reviews the role of arterial embolization (AE) in recurrent spontaneous hemarthroses (RSH) that are refractory to intensive prophylaxis (RIP) in people with hemophilia (PWH). There are six articles in the literature on the topic. The total number of AEs performed so far is 78 in 69 patients. Four second AEs were required (4/78), and a third AE in one (1/78). No serious adverse effects have been reported except for a pseudoaneurym (1/78) of the femoral artery at the puncture site (that eventually required surgical repair) and for one patient (1/78) that had recurrence of bleeding for whom surgical exploration was required...
December 16, 2016: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/28040482/schistosoma-antigens-downregulate-cxcl9-production-by-pbmc-of-htlv-1-infected-individuals
#2
Luciane Mota Lima, Luciana Santos Cardoso, Silvane Braga Santos, Ricardo Riccio Oliveira, Sérgio Costa Oliveira, Alfredo Miranda Góes, Alex Loukas, Maria Ilma Araujo
: HTLV-1 is the causal agent of Adult T cell Leukemia/lymphoma (ATLL) and HTLV-1-associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). The immune response to HTLV-1-infection is polarized to the Th1-type, and the presence of CXCL9/CXCL10 chemokines may lead to an increase in the recruitment of pro-inflammatory molecules in spinal cord tissue, contributing to the damage observed in the development of HAM/TSP. It has been observed that in chronic helminth-infections, such as schistosomiasis, there is a deviation toward the Th2/regulatory immune response...
December 28, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27976866/highly-selective-fluorescent-probe-for-imaging-h2se-in-living-cells-and-in-vivo-based-on-the-disulfide-bond
#3
Fanpeng Kong, Yuehui Zhao, Ziye Liang, Xiaojun Liu, Xiaohong Pan, Dongrui Luan, Kehua Xu, Bo Tang
Hydrogen selenide (H2Se) is an important metabolite of dietary Se compounds and has been implicated in various pathological and physiological processes. The development of highly sensitive and selective methods for the sensing of H2Se is therefore very important. Herein, we developed a fluorescent probe (hemicyanine (Hcy)-H2Se) for detecting H2Se based on a new H2Se-specific receptor unit, 1,2-dithiane-4,5-diol. Hcy-H2Se showed high selectivity toward H2Se over thiols (RSH), hydrogen sulfide (H2S), and selenocysteine (Sec) and was further exploited for the fluorescence imaging of H2Se both in living cells and in vivo...
December 15, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27936678/copper-ii-activation-of-nitrite-nitrosation-of-nucleophiles-and-generation-of-no-by-thiols
#4
Subrata Kundu, William Y Kim, Jeffery A Bertke, Timothy H Warren
Nitrite (NO2(-)) and nitroso compounds (E-NO, E = RS, RO, and R2N) in mammalian plasma and cells serve important roles in nitric oxide (NO) dependent as well as NO independent signaling. Employing an electron deficient β-diketiminato copper(II) nitrito complex [Cl2NNF6]Cu(κ(2)-O2N)·THF, thiols mediate reduction of nitrite to NO. In contrast to NO generation upon reaction of thiols at iron nitrite species, at copper this conversion proceeds through nucleophilic attack of thiol RSH on the bound nitrite in [Cu(II)](κ(2)-O2N) that leads to S-nitrosation to give the S-nitrosothiol RSNO and copper(II) hydroxide [Cu(II)]-OH...
December 22, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27867458/renal-subcapsular-hematoma-caused-by-hydrophilic-guide-wire-during-patent-ductus-arteriosus-closure
#5
Efe Edem, Harun Kilic, Alper Karacan, Ramazan Akdemir
Hydrophilic guide wire-associated renal subcapsular hematoma (RSH) during patent ductus arteriosus (PDA) closure is an extremely rare clinical condition. Herein, we present the case of a 16-year-old adolescent who suffered accidental hydrophilic 0.035-inch guide wire-related RSH during PDA closure. This RSH was diagnosed by computerized tomography and selective renal angiography. It was successfully treated by conservative treatment.
July 2016: Heart Views: the Official Journal of the Gulf Heart Association
https://www.readbyqxmd.com/read/27817165/obesity-is-not-a-descriptive-factor-for-oxidative-stress-and-viscosity-in-follicular-fluid-of-in-vitro-fertilization-patients
#6
B Yuksel, S Kilic, N Yilmaz, T Goktas, U Keskin, A Seven, M Ulubay, S Batioglu
BACKGROUND: Obesity's impact on micro-environmental oxidative stress and follicular fluid (FF) viscosity and whether or not it has any effect on in vitro fertilization (IVF) success is a matter of debate. AIMS: In this study, our aim was to evaluate the levels of oxidative stress markers and the FF viscosity in obese and non-obese patients. METHODS: Eighty norm-responder patients undergoing IVF were prospectively grouped according to their body mass indexes (BMI)...
November 5, 2016: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/27670814/signaling-by-sulfur-containing-molecules-quantitative-aspects
#7
Willem H Koppenol, Patricia L Bounds
There is currently interest in sulfur-containing molecules that may or may not play a role in signaling. We have collected relevant thermodynamic data, namely standard Gibbs energies of formation and electrode potentials at pH 7, and used these to construct a Frost diagram. Thermodynamic data not available in the literature could be estimated with reasonable confidence. At pH 7, the electrode potential of the RSS/RSS(-) couple is +0.68 V, 0.28 V less than that of the RS, H(+)/RSH couple. S2(-) is unstable with respect to HSS(-) and S2...
September 23, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27662445/a-retrospective-study-to-reveal-factors-associated-with-postoperative-shoulder-imbalance-in-patients-with-adolescent-idiopathic-scoliosis-with-double-thoracic-curve
#8
Choon Sung Lee, Chang Ju Hwang, Eic Ju Lim, Dong-Ho Lee, Jae Hwan Cho
OBJECTIVE Postoperative shoulder imbalance (PSI) is a critical consideration after corrective surgery for a double thoracic curve (Lenke Type 2); however, the radiographic factors related to PSI remain unclear. The purpose of this study was to identify the radiographic factors related to PSI after corrective surgery for adolescent idiopathic scoliosis (AIS) in patients with a double thoracic curve. METHODS This study included 80 patients with Lenke Type 2 AIS who underwent corrective surgery. Patients were grouped according to the presence [PSI(+)] or absence [PSI(-)] of shoulder imbalance at the final follow-up examination (differences of 20, 15, and 10 mm were used)...
December 2016: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/27651980/spontaneous-rectus-sheath-hematoma-in-pregnancy-complicated-by-the-development-of-transfusion-related-acute-lung-injury-a-case-report-and-review-of-the-literature
#9
Jennifer Gibbs, Firas Bridges, Kiran Trivedi, John Vullo
BACKGROUND: Rectus sheath hematoma (RSH) represents a rare, but serious cause of abdominal pain. CASE: Here we discuss the case of a healthy multigravida female who presented at 28 weeks gestation with spontaneous RSH. Conservative management with multiple blood transfusions led to the development of transfusion related acute lung injury (TRALI) and intensive care unit admission. She was managed with noninvasive ventilatory support, gradually improved, and was weaned of ventilation...
July 2016: American Journal of Perinatology Reports
https://www.readbyqxmd.com/read/27636328/achieving-predictive-description-of-molecular-conductance-by-using-a-range-separated-hybrid-functional
#10
Atsushi Yamada, Qingguo Feng, Austin Hoskins, Kevin D Fenk, Barry D Dunietz
The conductance of molecular bridges tends to be overestimated by computational studies in comparison to measured values. While this well-established trend may be related to difficulties for achieving robust bridges, the employed computational scheme can also contribute to this tendency. In particular, caveats of the traditional functionals employed in first-principles-based calculations can lead to discrepancies reflected in exaggerated conductance. Here, we show that by employing a range-separated hybrid functional the calculated values are within the same order as the measured conductance for all four considered cases...
October 12, 2016: Nano Letters
https://www.readbyqxmd.com/read/27614419/gc-ecnici-ms-analysis-of-s-nitrosothiols-and-nitroprusside-after-treatment-with-aqueous-sulphide-s-2-and-derivatization-with-pentafluorobenzyl-bromide-evidence-of-s-transnitrosylation-and-formation-of-nitrite-and-nitrate
#11
Dimitrios Tsikas, Mario Schmidt, Erik Hanff, Anke Böhmer
A GC-MS method is reported for the quantitative analysis of S-nitrosothiols (RSNO) derived from endogenous low- and high-molecular mass thiols (RSH) including hemoglobin, cysteine, glutathione, N-acetylcysteine, and the exogenous N-acetylcysteine ethyl ester. The method is based on the conversion of RSNO to nitrite by aqueous Na2S (S(2-)). (15)N-Labelled analogs (RS(15)NO) or (15)N-labelled nitrite and nitrate were used as internal standards. The nitrite ((14)NO2(-) and (15)NO2(-)) and nitrate (O(14)NO2(-) and O(15)NO2(-) anions were derivatised by pentafluorobenzyl (PFB) bromide (PFB-Br) in aqueous acetone and their PFB derivatives were separated by gas chromatography...
September 3, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27575868/reverse-split-hand-syndrome-dissociated-intrinsic-hand-muscle-atrophy-pattern-in-hirayama-disease-brachial-monomelic-amyotrophy
#12
Ravinder-Jeet Singh, Veeramani Preethish-Kumar, Kiran Polavarapu, Seena Vengalil, Chandrajit Prasad, Atchayaram Nalini
Preferential involvement of C7, C8, T1 level anterior horn cells is a typical feature in Hirayama disease/brachial monomelic amyotrophy (BMMA). There are no clinico-electrophysiological studies to substantiate the peculiar pattern of muscle involvement. Thirty subjects, 10 in each group of BMMA, amyotrophic lateral sclerosis (ALS) and age-matched normal healthy subjects underwent detailed clinical and electrophysiological testing. Results showed that the mean age at evaluation for BMMA and ALS patients was 25...
August 30, 2016: Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
https://www.readbyqxmd.com/read/27573437/effects-of-an-extract-of-celtis-aetnensis-tornab-strobl-twigs-on-human-colon-cancer-cell-cultures
#13
Rosaria Acquaviva, Valeria Sorrenti, Rosa Santangelo, Venera Cardile, Barbara Tomasello, Giuseppe Malfa, Luca Vanella, Andrea Amodeo, Carlo Genovese, Silvana Mastrojeni, Michela Pugliese, Monica Ragusa, Claudia Di Giacomo
Cancers of the digestive tract, in particular colorectal cancer (CRC), are among those most responsive to dietary modification. Research has shown that approximately 75% of all sporadic cases of CRC are directly influenced by diet. Many natural compounds have been investigated for their potential usefulness as cancer chemopreventive agents as they have been thought to suppress carcinogenesis mainly during the initiation phase due to their radical scavenger activity. Since there is an increasing interest in the in vivo protective effects of natural compounds contained in plants against oxidative damage involved in several human diseases such as cancer, the aim of the present research was to test the effects of a Celtis aetnensis (Tornab...
October 2016: Oncology Reports
https://www.readbyqxmd.com/read/27558436/identification-and-functional-analysis-of-rela-spot-homolog-rsh-genes-in-deinococcus-radiodurans
#14
Jinhui Wang, Ye Tian, Zhengfu Zhou, Liwen Zhang, Wei Zhang, Min Lin, Ming Chen
To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to H₂O₂, heat shock, and amino acid limitation...
December 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27516759/an-essential-role-for-p-ppgpp-in-the-integration-of-stress-tolerance-peptide-signaling-and-competence-development-in-streptococcus-mutans
#15
Justin Kaspar, Jeong N Kim, Sang-Joon Ahn, Robert A Burne
The microbes that inhabit the human oral cavity are subjected to constant fluctuations in their environment. To overcome these challenges and gain a competitive advantage, oral streptococci employ numerous adaptive strategies, many of which appear to be intertwined with the development of genetic competence. Here, we demonstrate that the regulatory circuits that control development of competence in Streptococcus mutans, a primary etiological agent of human dental caries, are integrated with key stress tolerance pathways by the molecular alarmone (p)ppGpp...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27494110/a-two-step-absorber-deposition-approach-to-overcome-shunt-losses-in-thin-film-solar-cells-using-tin-sulfide-as-a-proof-of-concept-material-system
#16
Vera Steinmann, Rupak Chakraborty, Paul H Rekemeyer, Katy Hartman, Riley E Brandt, Alex Polizzotti, Chuanxi Yang, Tom Moriarty, Silvija Gradečak, Roy G Gordon, Tonio Buonassisi
As novel absorber materials are developed and screened for their photovoltaic (PV) properties, the challenge remains to reproducibly test promising candidates for high-performing PV devices. Many early-stage devices are prone to device shunting due to pinholes in the absorber layer, producing "false-negative" results. Here, we demonstrate a device engineering solution toward a robust device architecture, using a two-step absorber deposition approach. We use tin sulfide (SnS) as a test absorber material. The SnS bulk is processed at high temperature (400 °C) to stimulate grain growth, followed by a much thinner, low-temperature (200 °C) absorber deposition...
August 31, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27403640/functional-complementation-analysis-fca-a-laboratory-exercise-designed-and-implemented-to-supplement-the-teaching-of-biochemical-pathways
#17
André O Hudson, Taylor C M Harkness, Michael A Savka
Functional complementation assay (FCA) is an in vivo assay that is widely used to elucidate the function/role of genes/enzymes. This technique is very common in biochemistry, genetics and many other disciplines. A comprehensive overview of the technique to supplement the teaching of biochemical pathways pertaining to amino acids, peptidoglycan and the bacterial stringent response is reported in this manuscript. Two cDNAs from the model plant organism Arabidopsis thaliana that are involved in the metabolism of lysine (L,L-diaminopimelate aminotransferase (dapL) and tyrosine aminotransferase (tyrB) involved in the metabolism of tyrosine and phenylalanine are highlighted...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27294771/repeated-sprint-training-in-hypoxia-induced-by-voluntary-hypoventilation-in-swimming
#18
Laurent Trincat, Xavier Woorons, Grégoire P Millet
PURPOSE: Repeated-sprint training in hypoxia (RSH) has been shown as an efficient method for improving repeated sprint ability (RSA) in team-sport players but has not been investigated in swimming. We assessed whether RSH with arterial desaturation induced by voluntary hypoventilation at low lung volume (VHL) could improve RSA to a greater extent than the same training performed under normal breathing (NB) conditions. METHODS: 16 competitive swimmers completed six sessions of repeated sprints (two sets of 16x15 m with 30 s send-off) either with VHL (RSH-VHL, n=8) or with NB (RSN, n=8)...
June 13, 2016: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/27240988/-p-ppgpp-and-the-bacterial-cell-cycle
#19
Aanisa Nazir, Rajendran Harinarayanan
Genes of the Rel/Spo homolog (RSH) superfamily synthesize and/or hydrolyse the modified nucleotides pppGpp/ ppGpp (collectively referred to as (p)ppGpp) and are prevalent across diverse bacteria and in plant chloroplasts. Bacteria accumulate (p)ppGpp in response to nutrient deprivation (generically called the stringent response) and elicit appropriate adaptive responses mainly through the regulation of transcription. Although at different concentrations (p)ppGpp affect the expression of distinct set of genes, the two well-characterized responses are reduction in expression of the protein synthesis machinery and increase in the expression of genes coding for amino acid biosynthesis...
June 2016: Journal of Biosciences
https://www.readbyqxmd.com/read/27140941/repeated-sprint-training-in-hypoxia-versus-normoxia-does-not-improve-performance-a-double-blind-and-cross-over-study
#20
David Montero, Carsten Lundby
PURPOSE: Few recent studies indicate that short-term repeated sprint training in hypoxia (RSH) improves repeated sprint (RS) performance compared with identical training under normoxic conditions (RSN) in endurance-trained subjects. Herein, we sought to determine the effects of RSH against RSN on RS performance under normoxic and moderate hypoxic conditions, using a randomized, double-blind and cross-over experimental design. METHODS: Fifteen endurance-trained male subjects (age=25±4 years) performed 4 weeks of RS training (3 sessions/week) in normobaric hypoxia (RSH, FiO2=13...
May 3, 2016: International Journal of Sports Physiology and Performance
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