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https://www.readbyqxmd.com/read/29340029/selenium-suppresses-inflammation-by-inducing-microrna-146a-in-staphylococcus-aureus-infected-mouse-mastitis-model
#1
Weijing Sun, Qi Wang, Yingfang Guo, Yifan Zhao, Xinying Wang, Zhenbiao Zhang, Ganzhen Deng, Mengyao Guo
We studied the effects of selenium (Se) on the inflammatory response in Staphylococcus aureus (S. aureus)-infected mastitis-model mice and mammary epithelial cells. In infected mice, Se elicited a dose-dependent decrease in mammary gland pathology that included inflammatory cell infiltration, disorganized acinar structure and mammary cell necrosis. Se decreased inflammation by increasing miR-146a and decreasing TLR2/6 as well as NF-κB and MAPK signaling pathways in mammary tissue from infected mice and mammary epithelial cells...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29335921/development-of-real-time-pcr-assay-for-the-detection-of-mycoplasma-bovis
#2
S Behera, R Rana, P K Gupta, D Kumar, Sonal, V Rekha, T R Arun, D Jena
Mycoplasma bovis is one of the important bovine mycoplasma involved in economically important clinical conditions like respiratory diseases, otitis media, and mastitis. The present study was undertaken with the objective of developing a SYBR Green dye-based real-time PCR assay targeting uvrC gene for the diagnosis of M. bovis. The analytical sensitivity and specificity of the assay were evaluated. The test showed 103-fold more sensitivity than conventional PCR and detected down to 100 fg level of DNA. It was found to be specific, as no cross reactivity was shown with other related bacteria and Mycoplasma species...
January 15, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29335029/estimating-the-proportion-of-clinically-diagnosed-infectious-and-non-infectious-animal-diseases-in-ganta-afeshum-woreda-eastern-tigray-zone-ethiopia
#3
Mebrahtu Tedla, Mebrahtu Gebreselassie
OBJECTIVE: This study was performed with the objective of identifying the proportion of emerging and endemic livestock diseases using cross sectional survey. RESULT: A total of 285 clinically diseased animals were presented to a veterinary clinic and diagnosed tentatively based on history, clinical sign, and simple laboratory diagnostics and from the study, actinomycosis (15.83%), mastitis (15%), tick infestation (10%), respiratory diseases (9.16%) and gastro intestinal parasitism (9...
January 15, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29333831/-mammary-hemangioma-in-an-infant-with-apparent-mastitis-case-report
#4
Cristina Rodríguez Jiménez, Concepción Míguez Navarro, Minia Campos Domínguez, Carmen Garrido Colino
Mammary pathology is infrequent during childhood. It is rare the probability of finding a breast mass in an infant. The most frequent possible diagnoses at this age are breast abscess, mastitis, breast engorgement due to maternal hormonal stimulation and hemangioma. Reaching the proper diagnosis is essential in order to apply a suitable treatment and avoid the potential disease complications. We present the case of a female infant having a mass in the right breast from birth. Initially the entity was treated as mastitis...
February 1, 2018: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/29331686/quercetin-decrease-somatic-cells-count-in-mastitis-of-dairy-cows
#5
Artur Burmańczuk, Piotr Hola, Andrzej Milczak, Tomasz Piech, Cezary Kowalski, Beata Wojciechowska, Tomasz Grabowski
Quercetin is a dietary flavonoid which has an effect on inflammation, angiogenesis and vascular inflammation. In several other flavonoids (e.g. kaempferol, astragalin, alpinetin, baicalein, indirubin), anti-inflammatory mechanism was proven by using mice mastitis model. The aim of the current study was pilot analysis of quercetin tolerability and its impact on somatic cells count (SCC) after multiple intramammary treatment on dairy cows with clinical mastitis. Based on SCC and clinical investigation, 9 dairy cows with clinical mastitis of one quarter were selected for the pilot study...
January 9, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29331471/symposium-review-novel-strategies-to-genetically-improve-mastitis-resistance-in-dairy-cattle
#6
P Martin, H W Barkema, L F Brito, S G Narayana, F Miglior
Mastitis is a disease of major economic importance to the dairy cattle sector because of the high incidence of clinical mastitis and prevalence of subclinical mastitis and, consequently, the costs associated with treatment, production losses, and reduced animal welfare. Disease-recording systems compiling data from a large number of farms are still not widely implemented around the world; thus, selection for mastitis resistance is often based on genetically correlated indicator traits such as somatic cell count (SCC), udder depth, and fore udder attachment...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331467/canadian-national-dairy-study-herd-level-milk-quality
#7
C A Bauman, H W Barkema, J Dubuc, G P Keefe, D F Kelton
The objective of this study was to estimate Canadian national milk quality parameters and estimate the bulk tank milk (BTM) prevalence of 4 mastitis pathogens, Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis, and Prototheca spp., on Canadian dairy farms. A questionnaire was sent to all Canadian dairy producers. Of the 1,062 producers who completed the questionnaire, 374 producers from across the country were visited and milking hygiene was assessed. Farm-level milk quality data for all Canadian dairy producers was collected from the provincial marketing boards and combined with the questionnaire and farm visit data...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331464/somatic-cell-count-based-selection-reduces-susceptibility-to-energy-shortage-during-early-lactation-in-a-sheep-model
#8
J Bouvier-Muller, C Allain, F Enjalbert, Y Farizon, D Portes, G Foucras, R Rupp
During the transition from late gestation to early lactation ruminants experience a negative energy balance (NEB), which is considered to increase susceptibility to mammary infections. Our previous study in 2 divergent lines of sheep selected for high and low somatic cell score (SCS) suggested an association between the response to NEB and genetic susceptibility to mastitis. Forty-eight early-lactation primiparous dairy ewes from the 2 SCS genetic lines were allocated to 2 homogeneous subgroups-an NEB group, which was energy restricted and received 60% of the energy requirements for 15 d, and a control-fed group-to obtain 4 balanced groups of 12 ewes: high-SCS positive energy balance, low-SCS positive energy balance, high-SCS NEB, and low-SCS NEB...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29330061/forsythoside-a-inhibited-s-aureus-stimulated-inflammatory-response-in-primary-bovine-mammary-epithelial-cells
#9
Jiali Zhang, Yue Zhang, Hailong Huang, Haiwei Zhang, Wei Lu, Guoqun Fu, Yunzhang Zhu
Forsythoside A (FTA), the major bioactive component extracted from Forsythiae fructus, has multiple biological properties especially anti-inflammatory property. Staphylococcus aureus (S. aureus), a Gram-positive organism, is one of most common pathogens that cause bovine mastitis. This study evaluated the anti-inflammatory effect of FTA in S. aureus-stimulated primary bovine mammary epithelial cells (bMEC). Primary bovine mammary epithelial cells were isolated from the mammary tissue of lactating cows and identified as bMEC...
January 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29325588/milk-flow-data-collected-routinely-in-an-automatic-milking-system-an-alternative-to-milking-time-testing-in-the-management-of-teat-end-condition
#10
Håvard Nørstebø, Amira Rachah, Gunnar Dalen, Odd Rønningen, Anne Cathrine Whist, Olav Reksen
BACKGROUND: Having a poor teat-end condition is associated with increased mastitis risk, hence avoiding milking machine settings that have a negative effect on teat-end condition is important for successful dairy production. Milking-time testing (MTT) can be used in the evaluation of vacuum conditions during milking, but the method is less suited for herds using automatic milking systems (AMS) and relationships with teat end condition is poorly described. This study aimed to increase knowledge on interpretation of MTT in AMS and to assess whether milk-flow data obtained routinely by an AMS can be useful for the management of teat-end health...
January 11, 2018: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29322122/granulomatous-mastitis-concurrence-with-breast-cancer
#11
Hasan Çalış, Asuman Kilitçi
Idiopathic Granulomatous Mastitis (IGM) is a rare, chronic, non-malignant and non-life-threatening breast disease. IGM may mimic carcinoma of the breast. This case report is about concurrence of chronic granulomatous mastitis with breast cancer. The important aspect of this case is that it is the 4th case where IGM and breast cancer are present concurrently.
January 2018: European Journal of Breast Health
https://www.readbyqxmd.com/read/29322116/efficiency-of-imaging-modalities-in-male-breast-disease-can-ultrasound-give-additional-information-for-assessment-of-gynecomastia-evolution
#12
Özgür Sarıca, A Nedim Kahraman, Enis Öztürk, Memik Teke
Objective: The purpose of this study is to present mammography and ultrasound findings of male breast lesions and to investigate the ability of diagnostic modalities in estimating the evolution of gynecomastia. Materials and Methods: Sixty-nine male patients who admitted to Taksim and Bakirkoy Education and Research Hospitals and underwent mammography (MG) and ultrasonography (US) imaging were retrospectively evaluated. Duration of symptoms and mammographic types of gynecomastia according to Appelbaum's classifications were evaluated, besides the sonographic findings in mammographic types of gynecomastia...
January 2018: European Journal of Breast Health
https://www.readbyqxmd.com/read/29321363/dominance-effects-estimation-of-tlr4-and-cacna2d1-genes-for-health-and-production-traits-using-logistic-regression
#13
Masoumeh Bagheri, Azadeh Zahmatkesh
Knowledge of nonadditive variance and genetic effects can be helpful in explaining the total genetic variation formost of the traits. The objective of this study was to estimate dominance effects of several single-nucleotide polymorphism (SNP) genotypes for the production traits and clinical mastitis residual (CMR), in Holstein dairy cattle in a case-control study. Records of 305 days lactation were obtained for production traits and CMR. Animals were selected based on extreme values for CMR from mixed model analyses...
December 2017: Journal of Genetics
https://www.readbyqxmd.com/read/29320762/corrected-and-republished-from-identification-of-peptidoglycan-hydrolase-constructs-with-synergistic-staphylolytic-activity-in-cow-s-milk
#14
Carolin T Verbree, Steven M Dätwyler, Susanne Meile, Fritz Eichenseher, David M Donovan, Martin J Loessner, Mathias Schmelcher
Peptidoglycan hydrolases (PGHs) have been suggested as novel therapeutics for the treatment of bovine mastitis. However, activity in the presence of cow's milk is an important requirement for drugs administered into the bovine udder. We have used a microtiter plate-based protocol to screen a library of >170 recombinant PGHs, including engineered bacteriophage endolysins, for enzymes with activity against Staphylococcus aureus in milk. Eight suitable PGH constructs were identified by this approach, and their efficacies against S...
January 1, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29319754/identification-and-antimicrobial-suceptibility-profile-of-bacteria-causing-bovine-mastitis-from-dairy-farms-in-pelotas-rio-grande-do-sul
#15
C H Freitas, J F Mendes, P V Villarreal, P R Santos, C L Gonçalves, H L Gonzales, P S Nascente
Mastitis is an inflammatory process of the udder tissue caused mainly by the bacteria Staphylococcus aureus. The indiscriminate use of antibiotics fosters conditions that favor the selection of resistant microorganisms, suppressing at the same time susceptible forms, causing a serious problem in dairy cattle. Given the importance in performing an antibiogram to select the most adequate antimicrobial therapy, the aim of this study was to identify bacteria isolated from cow's milk with mastitis, in dairy farms situated in the city of Pelotas, Rio Grande do Sul, and to determinate the susceptibility profile of these isolates against the antibiotics used to treat this illness...
January 8, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/29316704/using-longitudinal-assessment-on-extensively-managed-ewes-to-quantify-welfare-compromise-and-risks
#16
Carolina Munoz, Angus Campbell, Stuart Barber, Paul Hemsworth, Rebecca Doyle
This study examined variation in the welfare of extensively managed ewes and potential welfare risks. A total of 100 Merino ewes (aged 2-4 years) were individually identified and examined at three key stages: pregnancy, lactation and weaning. Eight animal-based welfare measures were used to assess welfare: flight distance, body condition score (BCS), fleece condition, skin lesions, tail length, dag score, lameness and mastitis. Data were analysed by ANOVA and McNemar's statistics. Overall, the average BCS of the group was in agreement with industry recommendations...
January 8, 2018: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/29316031/ultrasonographic-assessment-of-male-breast-diseases
#17
Xueyu Rong, Qiang Zhu, Wenxiu Jia, Teng Ma, Xixi Wang, Ning Guo, Hongtao Ji
Although rare and accounting for less than 1% of all breast cancers, the incidence of breast cancer in men has increased by 26% over the past few decades. Very little has been reported on the sonographic appearance of benign and malignant male breast conditions. The aim of this study was to describe the ultrasonographic features of male breast disease and the value of ultrasound in the evaluation of male breast disease. Between December 2006 and October 2014, ultrasound examinations were performed in 560 male patients presenting with enlargement of, pain in, and/or a lump in the breast...
January 8, 2018: Breast Journal
https://www.readbyqxmd.com/read/29315680/solexa-sequencing-and-custom-microrna-chip-reveal-repertoire-of-micrornas-in-mammary-gland-of-bovine-suffering-from-natural-infectious-mastitis
#18
Zhihua Ju, Qiang Jiang, Gang Liu, Xiuge Wang, Guojing Luo, Yan Zhang, Jibin Zhang, Jifeng Zhong, Jinming Huang
Identification of microRNAs (miRNAs), target genes and regulatory networks associated with innate immune and inflammatory responses and tissue damage is essential to elucidate the molecular and genetic mechanisms for resistance to mastitis. In this study, a combination of Solexa sequencing and custom miRNA chip approaches was used to profile the expression of miRNAs in bovine mammary gland at the late stage of natural infection with Staphylococcus aureus, a widespread mastitis pathogen. We found 383 loci corresponding to 277 known and 49 putative novel miRNAs, two potential mitrons and 266 differentially expressed miRNAs in the healthy and mastitic cows' mammary glands...
January 8, 2018: Animal Genetics
https://www.readbyqxmd.com/read/29315249/bacteriophage-%C3%AE-sa012-has-a-broad-host-range-against-staphylococcus-aureus-and-effective-lytic-capacity-in-a-mouse-mastitis-model
#19
Hidetomo Iwano, Yusuke Inoue, Takuji Takasago, Hironori Kobayashi, Takaaki Furusawa, Kotomi Taniguchi, Jumpei Fujiki, Hiroshi Yokota, Masaru Usui, Yasunori Tanji, Katsuro Hagiwara, Hidetoshi Higuchi, Yutaka Tamura
Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S...
January 9, 2018: Biology
https://www.readbyqxmd.com/read/29314139/validating-genome-wide-associated-signals-for-clinical-mastitis-in-german-holstein-cattle
#20
H Abdel-Shafy, R H Bortfeldt, M Reissmann, G A Brockmann
A validation study for six genomic regions previously identified by a genome-wide association study for somatic cell score was conducted with data of clinical mastitis in German Holstein cattle. Out of 10 tested SNPs, five on chromosomes 6, 13 and 19 were significantly associated with clinical mastitis (P < 0.05). Three SNPs on chromosomes 6 and 19 had the same direction of effect as those previously reported in the initial genome-wide association study for somatic cell score. The other two SNPs on chromosome 13 had opposite effects...
January 4, 2018: Animal Genetics
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