keyword
https://read.qxmd.com/read/38457376/modulation-of-nucleotide-metabolism-by-picornaviruses
#1
JOURNAL ARTICLE
Lonneke V Nouwen, Martijn Breeuwsma, Esther A Zaal, Chris H A van de Lest, Inge Buitendijk, Marleen Zwaagstra, Pascal Balić, Dmitri V Filippov, Celia R Berkers, Frank J M van Kuppeveld
Viruses actively reprogram the metabolism of the host to ensure the availability of sufficient building blocks for virus replication and spreading. However, relatively little is known about how picornaviruses-a large family of small, non-enveloped positive-strand RNA viruses-modulate cellular metabolism for their own benefit. Here, we studied the modulation of host metabolism by coxsackievirus B3 (CVB3), a member of the enterovirus genus, and encephalomyocarditis virus (EMCV), a member of the cardiovirus genus, using steady-state as well as 13C-glucose tracing metabolomics...
March 2024: PLoS Pathogens
https://read.qxmd.com/read/38331038/coxsackievirus-b3-hfmd-animal-models-in-syrian-hamster-and-rhesus-monkey
#2
JOURNAL ARTICLE
Suqin Duan, Wei Zhang, Yongjie Li, Yanyan Li, Yuan Zhao, Weihua Jin, Quan Liu, Mingxue Li, Wenting Sun, Lixiong Chen, Hongjie Xu, Jie Tang, Jinghan Hou, Zijun Deng, Fengmei Yang, Shaohui Ma, Zhanlong He
Coxsackievirus B3 (CVB3) is the pathogen causing hand, foot and mouth disease (HFMD), which manifests across a spectrum of clinical severity, ranging from mild to severe. However, traditional CVB3-infected mice studies have predominantly resulted in viral myocarditis and pancreatitis, failing to replicate HFMD symptoms. This has limited our understanding of CVB3 virus-host interaction. Meanwhile, the different clinical manifestations animal models could effectively accelerate the CVB3 novel therapies and vaccines development...
February 6, 2024: Virologica Sinica
https://read.qxmd.com/read/38309955/isg15-blocks-cardiac-glycolysis-and-ensures-sufficient-mitochondrial-energy-production-during-coxsackievirus-b3-infection
#3
JOURNAL ARTICLE
Clara Bredow, Fabien Thery, Eva Katrin Wirth, Sarah Ochs, Meike Kespohl, Gunnar Kleinau, Nicolas Kelm, Niclas Gimber, Jan Schmoranzer, Martin Voss, Karin Klingel, Joachim Spranger, Kostja Renko, Markus Ralser, Michael Mülleder, Arnd Heuser, Klaus-Peter Knobeloch, Patrick Scheerer, Jennifer Kirwan, Ulrike Brüning, Nikolaus Berndt, Francis Impens, Antje Beling
AIMS: Virus infection triggers inflammation and, may impose nutrient shortage to the heart. Supported by type I interferon (IFN) signaling, cardiomyocytes counteract infection by various effector processes, with the IFN-stimulated gene of 15 kDa (ISG15) system being intensively regulated and protein modification with ISG15 protecting mice Coxsackievirus B3 (CVB3) infection. The underlying molecular aspects how the ISG15 system affects the functional properties of respective protein substrates in the heart are unknown...
February 3, 2024: Cardiovascular Research
https://read.qxmd.com/read/38309307/miltefosine-reduces-coxsackievirus-b3-lethality-of-mice-with-enhanced-stat3-activation
#4
JOURNAL ARTICLE
Chun Yu Zhang, Cheng-Huei Hung, Yi-Ling Hsiao, Tung-Miao Chang, Yu-Chieh Su, Li-Chiu Wang, Shih-Min Wang, Shun-Hua Chen
Coxsackievirus B3 (CVB3), one serotype of enteroviruses, can induce fatal myocarditis and hepatitis in neonates, but both treatment and vaccine are unavailable. Few reports tested antivirals to reduce CVB3. Several antivirals were developed against other enterovirus serotypes, but these antivirals failed in clinical trials due to side effects and drug resistance. Repurposing of clinical drugs targeting cellular factors, which enhance viral replication, may be another option. Parasite and cancer studies showed that the cellular protein kinase B (Akt) decreases interferon (IFN), apoptosis, and interleukin (IL)-6-induced STAT3 responses, which suppress CVB3 replication...
February 1, 2024: Antiviral Research
https://read.qxmd.com/read/38289119/microrna-22-3p-displaces-critical-host-factors-from-the-5-utr-and-inhibits-the-translation-of-coxsackievirus-b3-rna
#5
JOURNAL ARTICLE
Priya Rani, Biju George, Sabarishree V, Somarghya Biswas, Madhurya V, Apala Pal, Raju S Rajmani, Saumitra Das
Coxsackievirus B3 (CVB3) is known to cause acute myocarditis and pancreatitis in humans. We investigated the microRNAs (miRNAs) that can potentially govern the viral life cycle by binding to the untranslated regions (UTRs) of CVB3 RNA. MicroRNA-22-3p was short-listed, as its potential binding site overlapped with the region crucial for recruiting internal ribosome entry site trans -acting factors (ITAFs) and ribosomes. We demonstrate that miR-22-3p binds CVB3 5' UTR, hinders recruitment of key ITAFs on viral mRNA, disrupts the spatial structure required for ribosome recruitment, and ultimately blocks translation...
January 30, 2024: Journal of Virology
https://read.qxmd.com/read/38279453/analysis-of-the-improvement-effect-of-astragalus-extract-on-oxidative-stress-injury-in-viral-myocarditis-through-stat3-il-6
#6
JOURNAL ARTICLE
Zhiyue Chang, Yanhui Zhang, Lu Wang, Huizhen Wang, Ling Tian, Juan Qiao
The objective of this study was to investigate the improvement effect of Astragalus (AS) extract on oxidative stress (OS) and inflammatory response of myocarditis (MYO) cells through the STAT3/IL-6 axis. For this purpose, The MYO model cells prepared by intervening cardiomyocyte HL-1 with Coxsackievirus B3 (CVB3) were divided into four groups: model group, as well as high- (H-), medium- (M-) and low-dose (L-) AS groups treated by 80, 40, and 20 μg/mL AS, respectively. Conventionally cultured cells were set as the normal group...
December 31, 2023: Cellular and Molecular Biology
https://read.qxmd.com/read/38278522/synergistic-viro-chemoimmunotherapy-in-breast-cancer-enabled-by-bioengineered-immunostimulatory-exosomes-and-dual-targeted-coxsackievirus-b3
#7
JOURNAL ARTICLE
Amirhossein Bahreyni, Yasir Mohamud, Sanaz Ashraf Nouhegar, Jingchun Zhang, Honglin Luo
Breast cancer's immunosuppressive environment hinders effective immunotherapy, but oncolytic viruses hold promise for addressing this challenge by targeting tumor cells and altering the microenvironment. Yet, neutralizing antibodies and immune clearance impede their clinical utility. This study explored microRNA-modified coxsackievirus B3 (miR-CVB3), an innovative oncolytic virus, and its potential in breast cancer treatment. It investigated miR-CVB3's impact on immune-related proteins and utilized exosomes as both protective shields and delivery carriers...
January 26, 2024: ACS Nano
https://read.qxmd.com/read/38275951/antiviral-mechanisms-of-saucerneol-from-saururus-chinensis-against-enterovirus-a71-coxsackievirus-a16-and-coxsackievirus-b3-role-of-mitochondrial-ros-and-the-sting-tkb-1-irf3-pathway
#8
JOURNAL ARTICLE
Jae-Hyoung Song, Seo-Hyeon Mun, Heejung Yang, Yong Soo Kwon, Seong-Ryeol Kim, Min-Young Song, Youngwook Ham, Hwa-Jung Choi, Won-Jin Baek, Sungchan Cho, Hyun-Jeong Ko
Enterovirus A71 (EV71), coxsackievirus A16 (CVA16), and coxsackievirus B3 (CVB3) are pathogenic members of the Picornaviridae family that cause a range of diseases, including severe central nervous system complications, myocarditis, and pancreatitis. Despite the considerable public health impact of these viruses, no approved antiviral treatments are currently available. In the present study, we confirmed the potential of saucerneol, a compound derived from Saururus chinensis , as an antiviral agent against EV71, CVA16, and CVB3...
December 21, 2023: Viruses
https://read.qxmd.com/read/38244740/exosome-co-delivery-of-a-sting-agonist-augments-immunogenicity-elicited-by-cvb3-vp1-vaccine-via-promoting-antigen-cross-presentation-of-cd8-dcs
#9
JOURNAL ARTICLE
Changwei Zhang, Qinghui Cao, Yuanyu Li, Juan Lu, Sidong Xiong, Yan Yue
The induction of a robust CD8+ T cell response is critical for the success of an antiviral vaccine. In this study, we incorporated a STING agonist (SA) 2'3'-cGAMP into a previously developed exosome-based CVB3 viral myocarditis vaccine (Exo-VP1) to enhance its ability to induce CD8+ T cell responses and immunoprotection. Our results showed that compared to free SA adjuvant, exosome-mediated co-delivery (ExoSA -VP1) significantly enhanced SA uptake by dendritic cells (DCs) and more potently stimulated DC maturation...
January 18, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38243751/folate-supports-il-25-induced-tuft-cell-expansion-following-enteroviral-infections
#10
JOURNAL ARTICLE
Ruining Lyu, Jing Wu, Yating He, Qiao You, Yajie Qian, Na Jiang, Yurong Cai, Deyan Chen, Zhiwei Wu
Intestinal tuft cells, a kind of epithelial immune cells, rapidly expand in response to pathogenic infections, which is associated with infection-induced interleukin 25 (IL-25) upregulation. However, the metabolic mechanism of IL-25-induced tuft cell expansion is largely unknown. Folate metabolism provides essential purine and methyl substrates for cell proliferation and differentiation. Thus, we aim to investigate the roles of folate metabolism playing in IL-25-induced tuft cell expansion by enteroviral infection and recombinant murine IL-25 (rmIL-25) protein-stimulated mouse models...
January 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38242503/activation-of-cgas-sting-suppresses-coxsackievirus-replication-via-interferon-dependent-signaling
#11
JOURNAL ARTICLE
Yasir Mohamud, Cathy Fu, Yiyun Michelle Fan, Yizhuo Lyanne Zhang, Jing Fei Carly Lin, Sinwoo Wendy Hwang, Zhihan Wang, Honglin Luo
Coxsackievirus B3 (CVB3) is a non-enveloped, single-stranded, positive RNA virus known for its role in provoking inflammatory diseases that affect the heart, pancreas, and brain, leading to conditions such as myocarditis, pancreatitis, and meningitis. Currently, there are no FDA-approved drugs treating CVB3 infection; therefore, identifying potential molecular targets for antiviral drug development is imperative. In this study, we examined the possibility of activating the cyclic GMP-AMP (cGAMP) synthase (cGAS)-stimulator of interferon genes (STING) pathway, a cytosolic DNA-sensing pathway that triggers a type-I interferon (IFN) response, in inhibiting CVB3 infection...
January 17, 2024: Antiviral Research
https://read.qxmd.com/read/38240287/tribbles-pseudokinase-3-promotes-enterovirus-a71-infection-via-dual-mechanisms
#12
JOURNAL ARTICLE
Huiqiang Wang, Ke Li, Boming Cui, Haiyan Yan, Shuo Wu, Kun Wang, Ge Yang, Jiandong Jiang, Yuhuan Li
Enterovirus A71 (EV-A71) is the main pathogen causing hand, foot and mouth disease (HFMD) in children and occasionally associated with neurological diseases such as aseptic meningitis, brainstem encephalitis (BE) and acute flaccid paralysis. We report here that cellular pseudokinase tribbles 3 (TRIB3) facilitates the infection of EV-A71 via dual mechanisms. In one hand, TRIB3 maintains the metabolic stability of scavenger receptor class B member 2 (SCARB2), the bona fide receptor of EV-A71, to enhance the infectious entry and spreading of the virus...
December 2024: Emerging Microbes & Infections
https://read.qxmd.com/read/38229002/camkii%C3%AE-stabilized-by-rna-n6-methyladenosine-reader-igf2bp2-boosts-coxsackievirus-b3-induced-myocardial-inflammation-via-interacting-with-tirap
#13
JOURNAL ARTICLE
Qingping Xiao, Lijuan Liu, Wei Qian, Ting Kang, Ru Ying, Jungang Nie
Calcium/calmodulin-dependent protein kinase II (CaMKII) has been demonstrated to be aberrantly activated in viral myocarditis (VMC), but the role of its subtype CaMKIIδ in VMC remains unclear.VMC mice and cardiomyocytes models were induced by Coxsackievirus B3 (CVB3) treatment. Mice that underwent sham surgery and saline-treated cardiomyocytes served as controls. Body weight, survival, left ventricular ejection fraction (LVEF), and fractional shortening (LVFS) were measured, and HE staining was performed to evaluate heart function in VMC mice model and sham control...
January 16, 2024: Journal of Cardiovascular Translational Research
https://read.qxmd.com/read/38196574/sex-differences-in-mitochondrial-gene-expression-during-viral-myocarditis
#14
Damian Di Florio, David Gorelov, Elizabeth McCabe, Danielle Beetler, Katie Shapiro, Katelyn Bruno, Isha Chekuri, Angita Jain, Emily Whelan, Gary Salomon, Sami Khatib, Natalie Bonvie-Hill, Presley Giresi, Varsini Balamurugan, Gabriel Weigel, Jessica Fliess, Ashley Darakjian, Brandy Edenfield, Christian Kocsis, Christopher McLeod, Leslie Cooper, Etienne Audet-Walsh, Michael Coronado, Jon Sin, DeLisa Fairweather
Background Myocarditis is an inflammation of the heart muscle most often caused by an immune response to viral infections. Sex differences in the immune response during myocarditis have been well described but upstream mechanisms in the heart that might influence sex differences in disease are not completely understood. Methods Male and female BALB/c wild type mice received an intraperitoneal injection of heart-passaged coxsackievirus B3 (CVB3) or vehicle control. Bulk-tissue RNA-sequencing was conducted to better understand sex differences in CVB3 myocarditis...
December 19, 2023: Research Square
https://read.qxmd.com/read/38169212/complement-components-regulates-ferroptosis-in-cvb3-viral-myocarditis-by-interatction-with-tfrc
#15
JOURNAL ARTICLE
Lu Yi, Yezhen Yang, Yanan Hu, Zhixiang Wu, Min Kong, Bojiao Zuoyuan, Xiaowei Xin, Zuocheng Yang
BACKGROUND: Dysregulated cell death machinery and an excessive inflammatory response in Coxsackievirus B3(CVB3)-infected myocarditis are hallmarks of an abnormal host response. Complement C4 and C3 are considered the central components of the classical activation pathway and often participate in the response process in the early stages of virus infection. METHODS: In our study, we constructed a mouse model of CVB3-related viral myocarditis via intraperitoneal injection of Fer-1 and detected myocarditis and ferroptosis markers in the mouse myocardium...
December 31, 2023: Free Radical Biology & Medicine
https://read.qxmd.com/read/38160539/m2-macrophage-exosome-derived-lncrna-ak083884-protects-mice-from-cvb3-induced-viral-myocarditis-through-regulating-pkm2-hif-1%C3%AE-axis-mediated-metabolic-reprogramming-of-macrophages
#16
JOURNAL ARTICLE
Yingying Zhang, Liangyu Zhu, Xueqin Li, Chang Ge, Weiya Pei, Mengying Zhang, Min Zhong, Xiaolong Zhu, Kun Lv
Viral myocarditis (VM) is a clinically common inflammatory disease. Accumulating literature has indicated that M2 macrophages protect mice from Coxsackievirus B3 (CVB3)-induced VM. However, mechanisms that underlie M2 macrophages alleviating myocardial inflammation remain largely undefined. We found that M2 macrophage-derived exosomes (M2-Exo) can effectively attenuate VM. The long non-coding RNA (lncRNA) AK083884 in M2-Exo was found to be involved in the regulation of macrophage polarization by exosome lncRNA sequencing combined with in vitro functional assays...
December 28, 2023: Redox Biology
https://read.qxmd.com/read/38127284/acetylation-of-foxo1-activates-bim-expression-involved-in-cvb3-induced-cardiomyocyte-apoptosis
#17
JOURNAL ARTICLE
Yanan Hu, Lu Yi, Yeyi Yang, Zhixiang Wu, Min Kong, Zhijuan Kang, Zuocheng Yang
Viral myocarditis (VMC) is the major reason for sudden cardiac death among both children and young adults. Of these, coxsackievirus B3 (CVB3) is the most common causative agent of myocarditis. Recently, the role of signaling pathways in the pathogenesis of VMC has been evaluated in several studies, which has provided a new perspective on identifying potential therapeutic targets for this hitherto incurable disease. In the present study, in vivo and in vitro experiments showed that CVB3 infection leads to increased Bim expression and triggers apoptosis...
December 21, 2023: Apoptosis: An International Journal on Programmed Cell Death
https://read.qxmd.com/read/38125579/antiviral-functionalization-of-cellulose-using-tannic-acid-and-tannin-rich-extracts
#18
JOURNAL ARTICLE
Marjo Haapakoski, Aleksei Emelianov, Dhanik Reshamwala, Mira Laajala, Jenni Tienaho, Petri Kilpeläinen, Jaana Liimatainen, Tuula Jyske, Mika Pettersson, Varpu Marjomäki
Due to seasonally appearing viruses and several outbreaks and present pandemic, we are surrounded by viruses in our everyday life. In order to reduce viral transmission, functionalized surfaces that inactivate viruses are in large demand. Here the endeavor was to functionalize cellulose-based materials with tannic acid (TA) and tannin-rich extracts by using different binding polymers to prevent viral infectivity of both non-enveloped coxsackievirus B3 (CVB3) and enveloped human coronavirus OC43 (HCoV-OC43)...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/38091826/cd4-t-em-cells-drive-the-progression-from-acute-myocarditis-to-dilated-cardiomyopathy-in-cvb3-induced-balb-c-mice
#19
JOURNAL ARTICLE
Yanlan Huang, Xiaojing Huang, Zhe Wei, Jingwei Dong, Jing Lu, Quan Tang, Feiyu Lu, Zhihong Cen, Weifeng Wu
Acute viral myocarditis can progress to chronic myocarditis leading to dilated cardiomyopathy (DCM). Persistent CD4+ T-cell-mediated autoimmunity triggered by infection plays a critical role in this progression. Increasing evidence demonstrates that effector memory CD4+ T (CD4+ TEM ) cells, a subset of memory CD4+ T cells, are crucial pathogenic mediators of many autoimmune diseases. However, the role of CD4+ TEM cells during the progression from acute viral myocarditis to DCM remains unknown. In this study, we observed an increase in CD4+ TEM cells both in the periphery and the heart, and memory CD4+ T cells were the predominant sources of IL-17A and IFN-γ among inflamed heart-infiltrating CD4+ T cells during the progression from acute myocarditis to chronic myocarditis and DCM in CVB3-induced BALB/c mice...
December 12, 2023: International Immunopharmacology
https://read.qxmd.com/read/37961130/a-viral-specific-cd4-t-cell-response-protects-female-mice-from-coxsackievirus-b3-infection
#20
Aryamav Pattnaik, Adeeba H Dhalech, Stephanie A Condotta, Caleb Corn, Martin J Richer, Laura M Snell, Christopher M Robinson
Biological sex plays an integral role in the immune response to various pathogens. The underlying basis for these sex differences is still not well defined. Here, we show that Coxsackievirus B3 (CVB3) induces a viral-specific CD4 + T cell response that can protect female mice from mortality. We found that CVB3 can induce expansion of CD62L lo CD4 + T cells in the mesenteric lymph node and spleen of female but not male mice as early as 5 days post-inoculation, indicative of activation. Using a recombinant CVB3 virus expressing a model CD4 + T cell epitope, we found that this response is due to viral antigen and not bystander activation...
October 27, 2023: bioRxiv
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