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https://www.readbyqxmd.com/read/28428823/combining-manipulation-of-transcription-factors-and-overexpression-of-the-target-genes-to-enhance-lignocellulolytic-enzyme-production-in-penicillium-oxalicum
#1
Liwei Gao, Zhonghai Li, Chengqiang Xia, Yinbo Qu, Meng Liu, Piao Yang, Lele Yu, Xin Song
BACKGROUND: Lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. The production of these enzymes is transcriptionally regulated by multiple transcription factors. We previously engineered Penicillium oxalicum for improved cellulase production via manipulation of three genes in the cellulase expression regulatory network. However, the potential of combinational engineering of multiple regulators and their targets at protein abundance and activity levels has not been fully explored...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28427395/protein-antigen-of-bird-related-hypersensitivity-pneumonitis-in-pigeon-serum-and-dropping
#2
Tsuyoshi Shirai, Haruhiko Furusawa, Asuka Furukawa, Yuki Ishige, Keisuke Uchida, Yasunari Miyazaki, Yoshinobu Eishi, Naohiko Inase
BACKGROUND: Avian antigen is a common cause of hypersensitivity pneumonitis (HP). Inhalation challenge with pigeon serum and pigeon dropping extract (PDE) elicits a hypersensitivity reaction in patients with bird-related hypersensitivity pneumonitis (BRHP), but the antigenic components in these materials have yet to be fully elucidated. METHOD: Pigeon serum, pigeon intestine homogenates, and PDE were immunoblotted with serum samples from 8 patients with BRHP, 2 patients with summer-type HP, 2 patients with humidifier lung, and 3 healthy volunteers...
April 20, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28427096/pde-2015-camp-signaling-protein-kinase-a-pka-and-phosphodiesterases-pdes-how-genetics-changed-the-way-we-look-at-one-of-the-most-studied-signaling-pathways
#3
Constantine A Stratakis, Fahrettin Kelestimur, Jérôme Bertherat
No abstract text is available yet for this article.
April 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28426333/existence-and-uniqueness-for-a-coupled-pde-model-for-motor-induced-microtubule-organization
#4
Thomas Hillen, Diana White, Gerda de Vries, Adriana Dawes
Microtubules (MTs) are protein filaments that provide structure to the cytoskeleton of cells and a platform for the movement of intracellular substances. The spatial organization of MTs is crucial for a cell's form and function. MTs interact with a class of proteins called motor proteins that can transport and position individual filaments, thus contributing to overall organization. In this paper, we study the mathematical properties of a coupled partial differential equation (PDE) model, introduced by White et al...
April 20, 2017: Journal of Biological Dynamics
https://www.readbyqxmd.com/read/28424739/rapid-screening-of-potential-phosphodiesterase-inhibitors-from-the-roots-of-ilex-pubescens-hook-et-arn-using-a-combination-of-ultrafiltration-and-lc-ms
#5
Zichen Liu, Zongtao Lin, Shizhong Chen, Lingjun Wang, Shaoxiang Xian
The cyclic nucleotide phosphodiesterase (PDE) plays an important role in regulating the levels of second messenger molecules cAMP and cGMP. Various PDE inhibitors have been successfully developed into drugs for targeted diseases. In addition, PDE inhibitors can also be found in different foods and natural medicines. In this study, ultrafiltration liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry (ultrafiltration LC-DAD-ESI-IT-TOF-MS) was applied to screen PDE inhibitors from the roots of Ilex pubescens Hook...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28421158/cytotoxicity-of-zardaverine-in-embryonal-rhabdomyosarcoma-from-a-costello-syndrome-patient
#6
Donna M Cartledge, Katherine M Robbins, Katherine M Drake, Rachel Sternberg, Deborah L Stabley, Karen W Gripp, E Anders Kolb, Katia Sol-Church, Andrew D Napper
Costello syndrome (CS) patients suffer from a very high 10% incidence of embryonal rhabdomyosarcoma (ERMS). As tools to discover targeted therapeutic leads, we used a CS patient-derived ERMS cell line (CS242 ERMS) harboring a homozygous p.G12A mutation in HRAS, and a control cell line derived from the same patient comprising non-malignant CS242 fibroblasts with a heterozygous p.G12A HRAS mutation. A library of 2,000 compounds with known pharmacological activities was screened for their effect on CS242 ERMS cell viability...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28415898/-31-phosphorus-magnetic-resonance-spectroscopy-of-the-liver-for-evaluating-inflammation-and-fibrosis-in-autoimmune-hepatitis
#7
Lauri Puustinen, Antti Hakkarainen, Reetta Kivisaari, Sonja Boyd, Urpo Nieminen, Martti Färkkilä, Nina Lundbom, Perttu Arkkila
BACKGROUND: Liver biopsy is the gold standard in evaluating inflammation and fibrosis in autoimmune hepatitis. AIMS: In search of non-invasive follow-up tools in autoimmune hepatitis, we evaluated (31)phosphorus magnetic resonance spectroscopy ((31)P MRS). METHODS: Twelve consecutive AIH patients (mean age 42.8 years, 10 women) underwent liver biopsy, routine laboratory liver function tests, which were compared to findings in (31)P MRS and transient elastography (TE)...
April 17, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28414190/engineered-polymeric-microspheres-obtained-by-multi-step-method-as-potential-systems-for-transarterial-embolization-and-intraoperative-imaging-of-hcc-preliminary-evaluation
#8
Elena Piera Porcu, Andrea Salis, Giovanna Rassu, Marcello Maestri, Jacopo Galafassi, Giovanna Bruni, Paolo Giunchedi, Elisabetta Gavini
The aim of this study was the development of novel fluorescent microspheres as embolic agent for transarterial embolization (TAE) of advanced stages of hepatocellular carcinoma (HCC). TAE is a minimally invasive procedure that induces tumour regression blocking the blood flow by injection of microparticles. The microspheres currently used in clinical application cannot be visualized in vivo. Surgeon could exploit the intraoperative detection of embolic agents during resection of the malignant mass. Biocompatible indocyanine green (ICG)-loaded microspheres (CAB-CS-ICG) were prepared using a multi-step method...
April 13, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28414142/doxofylline-does-not-increase-formoterol-induced-camp-nor-mkp-1-expression-in-asm-cells-resulting-in-lack-of-anti-inflammatory-effect
#9
Brijeshkumar S Patel, Michael J Kugel, Gina Baehring, Alaina J Ammit
The xanthine doxofylline has been examined in clinical trials and shown to have efficacy and greater tolerability than theophylline in asthma and chronic obstructive pulmonary disease. The 'novofylline' doxofylline has demonstrated bronchodilatory and anti-inflammatory actions in in vivo and ex vivo experimental models of respiratory disease. However, there are limited studies in vitro. We address this herein and examine whether doxofylline has anti-inflammatory impact on primary cultures of airway smooth muscle (ASM) cells...
April 13, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28406920/experimental-and-mathematical-analysis-of-camp-nanodomains
#10
Christian Lohse, Andreas Bock, Isabella Maiellaro, Annette Hannawacker, Lothar R Schad, Martin J Lohse, Wolfgang R Bauer
In their role as second messengers, cyclic nucleotides such as cAMP have a variety of intracellular effects. These complex tasks demand a highly organized orchestration of spatially and temporally confined cAMP action which should be best achieved by compartmentalization of the latter. A great body of evidence suggests that cAMP compartments may be established and maintained by cAMP degrading enzymes, e.g. phosphodiesterases (PDEs). However, the molecular and biophysical details of how PDEs can orchestrate cAMP gradients are entirely unclear...
2017: PloS One
https://www.readbyqxmd.com/read/28406621/discovery-of-selective-phosphodiesterase-1-inhibitors-with-memory-enhancing-properties
#11
Brian Dyck, Bryan Branstetter, Tawfik Gharbaoui, Andrew R Hudson, J Guy Breitenbucher, Laurent Gomez, Iriny Botrous, Tami Marrone, Richard Barido, Charles K Allerston, E Peder Cedervall, Rui Xu, Vandana Sridhar, Ryan Barker, Kathleen Aertgeerts, Kara Schmelzer, David Neul, Dong Lee, Mark Eben Massari, Carsten B Andersen, Kristen Sebring, Xianbo Zhou, Robert Petroski, James Limberis, Martin Augustin, Lawrence E Chun, Thomas E Edwards, Marco Peters, Ali Tabatabaei
A series of potent thienotriazolopyrimidinone-based PDE1 inhibitors was discovered. X-ray crystal structures of example compounds from this series in complex with the catalytic domain of PDE1B and PDE10A were determined, allowing optimization of PDE1B potency and PDE selectivity. Reduction of hERG affinity led to greater than a 3000-fold selectivity for PDE1B over hERG. 6-(4-Methoxybenzyl)-9-((tetrahydro-2H-pyran-4-yl)methyl)-8,9,10,11-tetrahydropyrido[4',3':4,5]thieno[3,2-e][1,2,4]triazolo[1,5-c]pyrimidin-5(6H)-one was identified as an orally bioavailable and brain penetrating PDE1B enzyme inhibitor with potent memory-enhancing effects in a rat model of object recognition memory...
April 13, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28405996/ebstein-s-anomaly-with-paradoxical-embolism-to-lower-limb
#12
Gouranga Santra, Shinjan Patra
Ebstein's anomaly (EA) is a rare congenital heart disease. Paradoxical embolism (PDE) may occur in EA due to associated atrial septal defect (ASD). An 18 year old male with EA presented with left lower limb ischemia due to PDE to the limb. No features of cerebral embolism was present. PDE is a potential complication of EA. High level of awareness is needed for early diagnosis of PDE in EA to save a limb in case of embolism to the extremity.
December 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28405794/a-model-for-the-spread-of-an-invasive-weed-tradescantia-fluminensis
#13
Alexandra B Hogan, Mary R Myerscough
Tradescantia fluminensis is an invasive weed and a serious threat to native forests in eastern Australia and New Zealand. Current methods of eradication are often ineffective, so understanding the growth mechanisms of Tradescantia is important in formulating better control strategies. We present a partial differential equation (PDE) model for Tradescantia growth and spatial proliferation that accounts for Tradescantia's particular creeping and branching morphology, and the impact of self-shading on plant growth...
April 12, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28405029/protein-kinase-enzymes-in-the-human-vagina-relation-to-key-mediators-of-the-cyclic-amp-and-cyclic-gmp-pathways
#14
S Ückert, J Sonnenberg, J E Sonnenberg, W Kauffels, K Albrecht, M A Kuczyk, P Hedlund
Aside from phosphodiesterase (PDE) isoenzymes, protein kinases (cAK=cyclic AMP-binding protein kinase, cGK=cyclic GMP-binding protein kinase) have also been identified as important receptors for cyclic nucleotides. A significance of protein kinases in the control of the function of the male and female reproductive tract has been suggested; however, up until today, only a few approaches have addressed these enzymes in female genital tissues. The present study aimed to investigate by means of biochemical and immunohistochemical methods the expression of cAK and cGK...
April 13, 2017: International Journal of Impotence Research
https://www.readbyqxmd.com/read/28404873/sulfhydration-associated-phosphodiesterase-5a-dimerization-mediates-vasorelaxant-effect-of-hydrogen-sulfide
#15
Yan Sun, Yaqian Huang, Wen Yu, Siyao Chen, Qiuyu Yao, Chunyu Zhang, Dingfang Bu, Chaoshu Tang, Junbao Du, Hongfang Jin
The study was designed to examine if the vasorelaxant effect of hydrogen sulfide was mediated by sulfhydration-associated phosphodiesterase (PDE) 5A dimerization. The thoracic aorta of rat was separated and the vasorelaxant effects were examined with in vitro vascular perfusion experiments. The dimerization and sulfhydration of PDE 5A and soluble guanylatecyclase (sGC) were measured. PDE 5A and protein kinase G (PKG) activities were tested. Intracellular cGMP content was detected by enzyme-linked immunosorbent assay (ELISA)...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28396589/sphingosine-kinase-1-interacting-protein-is-a-novel-regulator-of-glucose-stimulated-insulin-secretion
#16
Yu Wang, Shin-Ichi Harashima, Yanyan Liu, Ryota Usui, Nobuya Inagaki
Glucose-stimulated insulin secretion (GSIS) is essential in keeping blood glucose levels within normal range. GSIS is impaired in type 2 diabetes, and its recovery is crucial in treatment of the disease. We find here that sphingosine kinase 1-interacting protein (SKIP, also called Sphkap) is highly expressed in pancreatic β-cells but not in α-cells. Intraperitoneal glucose tolerance test showed that plasma glucose levels were decreased and insulin levels were increased in SKIP(-/-) mice compared to SKIP(+/+) mice, but exendin-4-enhanced insulin secretion was masked...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396013/specific-sirt1-activator-mediated-improvement-in-glucose-homeostasis-requires-sirt1-independent-activation-of-ampk
#17
Sung-Jun Park, Faiyaz Ahmad, Jee-Hyun Um, Alexandra L Brown, Xihui Xu, Hyeog Kang, Hengming Ke, Xuesong Feng, James Ryall, Andrew Philp, Simon Schenk, Myung K Kim, Vittorio Sartorelli, Jay H Chung
The specific Sirt1 activator SRT1720 increases mitochondrial function in skeletal muscle, presumably by activating Sirt1. However, Sirt1 gain of function does not increase mitochondrial function, which raises a question about the central role of Sirt1 in SRT1720 action. Moreover, it is believed that the metabolic effects of SRT1720 occur independently of AMP-activated protein kinase (AMPK), an important metabolic regulator that increases mitochondrial function. Here, we show that SRT1720 activates AMPK in a Sirt1-independent manner and SRT1720 activates AMPK by inhibiting a cAMP degrading phosphodiesterase (PDE) in a competitive manner...
March 14, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28394532/-phosphodiesterase-type-5-inhibitors-in-the-treatment-of-erectile-dysfunction-past-present-and-future
#18
S I Gamidov, R I Ovchinnikov, A Yu Popova, S Kh Izhbaev
This review article describes the differences in efficacy and side effects between available phosphodiesterase type 5 (PDE-5) inhibitors used for treating erectile dysfunction. The most studied PDE-5 inhibitor is sildenafil.
April 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/28389413/influence-of-cell-confluence-on-the-camp-signalling-pathway-in-vascular-smooth-muscle-cells
#19
M Belacel-Ouari, L Zhang, F Hubert, R Assaly, R Gerbier, R Jockers, F Dauphin, P Lechêne, R Fischmeister, B Manoury, V Leblais
The influence of cell confluence on the β-adrenoceptor (β-AR)/cAMP/phosphodiesterase (PDE) pathway was investigated in cultured rat aortic smooth muscle cells (RASMCs). Cells were plated either at low density (LD: 3·10(3)cells/cm(2)) or high density (HD: 3·10(4)cells/cm(2)) corresponding to non-confluent or confluent cells, respectively, on the day of experiment. β-AR-stimulated cAMP was monitored in real-time using the fluorescence resonance energy transfer (FRET)-based cAMP sensor, Epac2-camps. A brief application (15s) of the β-AR agonist isoprenaline (Iso) induced a typical transient FRET signal, reflecting cAMP production followed by its rapid degradation...
April 4, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28382232/resveratrol-enhances-the-inotropic-effect-but-inhibits-the-proarrhythmic-effect-of-sympathomimetic-agents-in-rat-myocardium
#20
Jesús Hernandez-Cascales
BACKGROUND: Resveratrol is a cardioprotective agent with known antiarrhythmic effects that has recently been shown to inhibit phosphodiesterase (PDE) enzyme activity. Thus, it is possible that resveratrol increases the inotropic effect of sympathomimetic agents, as PDE inhibitors do but, unlike other PDE inhibitors, its effect may not be accompanied by proarrhythmia due to its antiarrhythmic action. This work is aimed to test this hypothesis. METHODS: This is an "in vitro" concentration-response relationship study...
2017: PeerJ
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