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Daniel J Kao, Kevin Franzese, Young Il Seo, Neil V Mandalaywala, Kunal Oak, Alex Moroz, Tracy Espiritu, Derek J Ho
No abstract text is available yet for this article.
September 2016: PM & R: the Journal of Injury, Function, and Rehabilitation
Derek J Ho, Daniel J Kao, Tracy Espiritu
No abstract text is available yet for this article.
September 2016: PM & R: the Journal of Injury, Function, and Rehabilitation
Tracy Espiritu McKay, Matina Balou, Daniel J Kao, Derek J Ho, Jeffrey Cohen, Eduardo D Rodriguez
No abstract text is available yet for this article.
September 2016: PM & R: the Journal of Injury, Function, and Rehabilitation
Nawreen Rahman, Lavoisier Ramos-Espiritu, Teresa A Milner, Jochen Buck, Lonny R Levin
Lysosomes, the degradative organelles of the endocytic and autophagic pathways, function at an acidic pH. Lysosomes are acidified by the proton-pumping vacuolar ATPase (V-ATPase), but the molecular processes that set the organelle's pH are not completely understood. In particular, pH-sensitive signaling enzymes that can regulate lysosomal acidification in steady-state physiological conditions have yet to be identified. Soluble adenylyl cyclase (sAC) is a widely expressed source of cAMP that serves as a physiological pH sensor in cells...
October 2016: Journal of General Physiology
Salvador J Jorgensen, A Peter Klimley, Arturo Muhlia-Melo, Steven G Morgan
Seamounts have generally been identified as locations that can promote elevated productivity, biomass and predator biodiversity. These properties attract seamount-associated fisheries where elevated harvests can be obtained relative to surrounding areas. There exists large variation in the geological and oceanographic environment among the thousands of locations that fall within the broad definition of seamount. Global seamount surveys have revealed that not all seamounts are hotspots of biodiversity, and there remains a strong need to understand the mechanisms that underlie variation in species richness observed...
2016: PeerJ
Kimberly Cornelio, Rafael Atillo Espiritu, Yasuto Todokoro, Shinya Hanashima, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga
Theonellamide A (TNM-A) is an antifungal bicyclic dodecapeptide isolated from a marine sponge Theonella sp. Previous studies have shown that TNM-A preferentially binds to 3β-hydroxysterol-containing membranes and disrupts membrane integrity. In this study, several (1)H NMR-based experiments were performed to investigate the interaction mode of TNM-A with model membranes. First, the aggregation propensities of TNM-A were examined using diffusion ordered spectroscopy; the results indicate that TNM-A tends to form oligomeric aggregates of 2-9 molecules (depending on peptide concentration) in an aqueous environment, and this aggregation potentially influences the membrane-disrupting activity of the peptide...
November 1, 2016: Bioorganic & Medicinal Chemistry
Petr Stepanov, Yafis Barlas, Tim Espiritu, Shi Che, Kenji Watanabe, Takashi Taniguchi, Dmitry Smirnov, Chun Ning Lau
The copresence of multiple Dirac bands in few-layer graphene leads to a rich phase diagram in the quantum Hall regime. Using transport measurements, we map the phase diagram of BN-encapsulated ABA-stacked trilayer graphene as a function charge density n, magnetic field B, and interlayer displacement field D, and observe transitions among states with different spin, valley, orbital, and parity polarizations. Such a rich pattern arises from crossings between Landau levels from different subbands, which reflect the evolving symmetries that are tunable in situ...
August 12, 2016: Physical Review Letters
Lavoisier Ramos-Espiritu, Silke Kleinboelting, Felipe A Navarrete, Antonio Alvau, Pablo E Visconti, Federica Valsecchi, Anatoly Starkov, Giovanni Manfredi, Hannes Buck, Carolina Adura, Jonathan H Zippin, Joop van den Heuvel, J Fraser Glickman, Clemens Steegborn, Lonny R Levin, Jochen Buck
The prototypical second messenger cAMP regulates a wide variety of physiological processes. It can simultaneously mediate diverse functions by acting locally in independently regulated microdomains. In mammalian cells, two types of adenylyl cyclase generate cAMP: G-protein-regulated transmembrane adenylyl cyclases and bicarbonate-, calcium- and ATP-regulated soluble adenylyl cyclase (sAC). Because each type of cyclase regulates distinct microdomains, methods to distinguish between them are needed to understand cAMP signaling...
October 2016: Nature Chemical Biology
Lavoisier Ramos-Espiritu, Ana Diaz, Charlee Nardin, Anthony J Saviola, Fiona Shaw, Tamar Plitt, Xia Yang, Jedd Wolchok, Edyta C Pirog, Garrett Desman, Andrea Sboner, Tuo Zhang, Jenny Xiang, Taha Merghoub, Lonny R Levin, Jochen Buck, Jonathan H Zippin
cAMP signaling pathways can both stimulate and inhibit the development of cancer; however, the sources of cAMP important for tumorigenesis remain poorly understood. Soluble adenylyl cyclase (sAC) is a non-canonical, evolutionarily conserved, nutrient- and pH-sensing source of cAMP. sAC has been implicated in the metastatic potential of certain cancers, and it is differentially localized in human cancers as compared to benign tissues. We now show that sAC expression is reduced in many human cancers. Loss of sAC increases cellular transformation in vitro and malignant progression in vivo...
June 15, 2016: Oncotarget
Luz Patricia Díaz Heredia, Alba Idaly Muñoz Sanchez
OBJECTIVE: to demonstrate the relations among vulnerability, self-transcendence and well-being in the young adult population and the effect of each of these variables on the adoption of low-risk consumption conducts. METHOD: quantitative and cross-sectional correlation study using structural equations analysis to test the relation among the variables. RESULTS: an inverse relation was evidenced between vulnerability to alcohol consumption and spiritual transcendence (β-0...
June 7, 2016: Revista Latino-americana de Enfermagem
Parashar Thapa, Chino C Cabalteja, Edwin E Philips, Michael J Espiritu, Steve Peigneur, Bea G Mille, Jan Tytgat, Theodore R Cummins, Jon-Paul Bingham
Tert-butyloxycarbonyl (t-Boc)-based native chemical ligation (NCL) techniques commonly employ hydrogen fluoride (HF) to create the thioester fragment required for the ligation process. Our research aimed to assess the replacement of HF with Trifluoromethanesulfonic acid (TFMSA). Here we examined a 33 amino acid test peptide, Huwentoxin-I (HwTx-I) as a novel candidate for our TFMSA cleavage protocol. Structurally HwTx-I has an X-Cys(16) -Cys(17) -X sequence mid-region, which makes it an ideal candidate for NCL...
September 2016: Biopolymers
Jodian A Brown, Marie V Espiritu, Joel Abraham, Ian F Thorpe
The identification of ligand-binding sites is often the first step in drug targeting and design. To date there are numerous computational tools available to predict ligand binding sites. These tools can guide or mitigate the need for experimental methods to identify binding sites, which often require significant resources and time. Here, we evaluate four ligand-binding site predictor (LBSP) tools for their ability to predict allosteric sites within the Hepatitis C Virus (HCV) polymerase. Our results show that the LISE LBSP is able to identify all three target allosteric sites within the HCV polymerase as well as a known allosteric site in the Coxsackievirus polymerase...
August 15, 2016: Virus Research
Daniella Espiritu, Daisuke Onohara, Kanika Kalra, Eric L Sarin, Muralidhar Padala
Mitral regurgitation is a common cardiac valve lesion, developing from primary lesions of the mitral valve or secondary to cardiomyopathies. Moderate or higher severity of mitral regurgitation imposes significant volume overload on the left ventricle, causing permanent structural and functional deterioration of the myocardium and heart failure. Timely correction of regurgitation is essential to preserve cardiac function, but surgical mitral valve repair is often delayed due to the risks of open heart surgery...
May 27, 2016: Annals of Biomedical Engineering
Rafael A Espiritu
Amphidinol 3 (AM3) and theonellamide A (TNM-A) are potent antifungal compounds produced by the dinoflagellate Amphidinium klebsii and the sponge Theonella spp., respectively. Both of these metabolites have been demonstrated to interact with membrane lipids ultimately resulting in a compromised bilayer integrity. In this report, the activity of AM3 and TNM-A in ternary lipid mixtures composed of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC):brain sphingomyelin:cholesterol at a mole ratio of 1:1:1 or 3:1:1 exhibiting lipid rafts coexistence is presented...
May 9, 2016: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
S Young, W R G Perry, B Leodoro, V Nosa, I Bissett, J A Windsor, A J Dare
BACKGROUND: The Pacific island nation of Vanuatu faces a number of challenges in delivering surgical care to its population. We aimed to understand and document the barriers, opportunities and required actions to improve surgical care in the country using a mixed methods analysis which incorporated the perspectives of local health stakeholders. METHODS: A baseline quantitative assessment of surgical capacity in Vanuatu was carried out using the WHO situational analysis tool...
August 2016: World Journal of Surgery
Vyas Ramanan, Kartik Trehan, Mei-Lyn Ong, Joseph M Luna, Hans-Heinrich Hoffmann, Christine Espiritu, Timothy P Sheahan, Hamsika Chandrasekar, Robert E Schwartz, Kathleen S Christine, Charles M Rice, Alexander van Oudenaarden, Sangeeta N Bhatia
Hepatitis C virus (HCV) is a positive single-stranded RNA virus of enormous global health importance, with direct-acting antiviral therapies replacing an immunostimulatory interferon-based regimen. The dynamics of HCV positive and negative-strand viral RNAs (vRNAs) under antiviral perturbations have not been studied at the single-cell level, leaving a gap in our understanding of antiviral kinetics and host-virus interactions. Here, we demonstrate quantitative imaging of HCV genomes in multiple infection models, and multiplexing of positive and negative strand vRNAs and host antiviral RNAs...
July 2016: Virology
Elena Arutyunova, Cameron C Smithers, Valentina Corradi, Adam C Espiritu, Howard S Young, D Peter Tieleman, M Joanne Lemieux
Rhomboids are ubiquitous intramembrane serine proteases involved in various signaling pathways. While the high-resolution structures of the Escherichia coli rhomboid GlpG with various inhibitors revealed an active site comprised of a serine-histidine dyad and an extensive oxyanion hole, the molecular details of rhomboid catalysis were unclear because substrates are unknown for most of the family members. Here we used the only known physiological pair of AarA rhomboid with its psTatA substrate to decipher the contribution of catalytically important residues to the reaction rate enhancement...
September 1, 2016: Biological Chemistry
Emilia Campos de Carvalho, Aline Helena Appoloni Eduardo, Adilson Romanzini, Talita Prado Simão, Cristina Mara Zamarioli, Danielle Cristina Garbuio, Tracy Heather Herdman
: The accurate identification of the nursing diagnoses and the corresponding measurement of their indicators will depend on the elements in the standardized language systems. OBJECTIVE: To analyze the correspondence between elements of four NANDA-I nursing diagnoses and outcomes suggested by the nursing outcomes classification (NOC) for the resolution of these diagnoses. METHOD: A methodological study consisting of cross-mapping was performed in the first stage between definitions, defining characteristics (DCs), and related factors (RFs) of impaired oral mucous membrane, impaired tissue integrity, spiritual distress, and delayed surgical recovery and outcome definitions and indicators to measure results of diagnoses resolution and an analysis of its relevance by experts in the second stage...
April 5, 2016: International Journal of Nursing Knowledge
Rafael Atillo Espiritu, Kimberly Cornelio, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga
Theonellamides (TNMs) are antifungal and cytotoxic bicyclic dodecapeptides derived from the marine sponge Theonella sp. These peptides specifically bind to 3β-hydroxysterols, resulting in 1,3-β-D-glucan overproduction and membrane damage in yeasts. The inclusion of cholesterol or ergosterol in phosphatidylcholine membranes significantly enhanced the membrane affinity of theonellamide A (TNM-A) because of its direct interaction with 3β-hydroxyl groups of sterols. To better understand TNM-induced membrane alterations, we investigated the effects of TNM-A on liposome morphology...
June 2016: Biochimica et Biophysica Acta
Silke Kleinboelting, Lavoisier Ramos-Espiritu, Hannes Buck, Laureen Colis, Joop van den Heuvel, J Fraser Glickman, Lonny R Levin, Jochen Buck, Clemens Steegborn
The signaling molecule cAMP regulates functions ranging from bacterial transcription to mammalian memory. In mammals, cAMP is synthesized by nine transmembrane adenylyl cyclases (ACs) and one soluble AC (sAC). Despite similarities in their catalytic domains, these ACs differ in regulation. Transmembrane ACs respond to G proteins, whereas sAC is uniquely activated by bicarbonate. Via bicarbonate regulation, sAC acts as a physiological sensor for pH/bicarbonate/CO2, and it has been implicated as a therapeutic target, e...
April 29, 2016: Journal of Biological Chemistry
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