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https://www.readbyqxmd.com/read/29775825/binding-capacity-of-mannose-binding-lectin-mbl-is-associated-with-the-severity-of-chronic-chagas-cardiomyopathy
#1
Elisa de A N Azevedo, Silvana Barreto, Raul Emídio de Lima, Romero Henrique Teixeira, George Diniz, Wilson Oliveira, Maria da Glória A M Cavalcanti, Yara M Gomes, Patrícia M M de F Moura, Clarice N L Morais
Chagas disease (CD) is a global problem. Currently, it affects approximately 15 million individuals in Latin America. It is well know that the human immune response is related to different clinical manifestations. Mannose binding lectin (MBL) plays an important role in innate immunity, and it mediates the phagocytosis and complement-mediated destruction of pathogens. The binding capacity is enhanced by the oligomerization of MBL. In this study, we evaluated the serum concentration and the binding capacity of MBL in patients with chronic chagasic cardiomyopathy...
May 15, 2018: Parasitology International
https://www.readbyqxmd.com/read/29774533/analysis-of-type-2-diabetes-and-obesity-genetic-variants-in-mexican-pima-indians-marked-allelic-differentiation-among-amerindians-at-hla
#2
Wen-Chi Hsueh, Peter H Bennett, Julian Esparza-Romero, Rene Urquidez-Romero, Mauro E Valencia, Eric Ravussin, Robert C Williams, William C Knowler, Leslie J Baier, Leslie O Schulz, Robert L Hanson
Prevalence of diabetes and obesity in Mexican Pima Indians is low, while prevalence in US Pima Indians is high. Although lifestyle likely accounts for much of the difference, the role of genetic factors is not well explored. To examine this, we genotyped 359 single nucleotide polymorphisms, including established type 2 diabetes and obesity variants from genome-wide association studies (GWAS) and 96 random markers, in 342 Mexican Pimas. A multimarker risk score of obesity variants was associated with body mass index (BMI; β = 0...
May 17, 2018: Annals of Human Genetics
https://www.readbyqxmd.com/read/29774523/acute-and-subacute-antidiabetic-studies-of-enp-9-a-new-1-5-diarylpyrazole-derivative
#3
Eduardo Hernández-Vázquez, Sandra Young-Peralta, Litzia Cerón-Romero, Sara García-Jiménez, Samuel Estrada-Soto
OBJECTIVES: To explore the antihyperglycaemic and antidiabetic effects and to determine the acute toxicity of 5-(4-chlorophenyl)-1-(2,4-dichloro-phenyl)-4-methyl-N-(piperidin-1-yl)-1H-pyrazole-3-carboxamide (ENP-9). METHODS: The antihyperglycaemic effect of ENP-9 (50 mg/kg) was determined by oral glucose tolerance test (OGTT). Also, the acute (16, 50 and 160 mg/kg) and subacute (50 mg/kg/day for 10 days) antidiabetic effects of ENP-9 were determined. After subacute treatment, blood samples were analysed to determine glucose and lipid profiles...
May 17, 2018: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/29774098/establishment-of-primary-cell-culture-and-an-intracranial-xenograft-model-of-pediatric-ependymoma-a-prospect-for-therapy-development-and-understanding-of-tumor-biology
#4
Lorena Favaro Pavon, Tatiana Tais Sibov, Silvia Regina Caminada de Toledo, Daniela Mara de Oliveira, Francisco Romero Cabral, Jean Gabriel de Souza, Pamela Boufleur, Luciana C Marti, Jackeline Moraes Malheiros, Edgar Ferreira da Cruz, Fernando F Paiva, Suzana M F Malheiros, Manoel A de Paiva Neto, Alberto Tannús, Sérgio Mascarenhas de Oliveira, Nasjla Saba Silva, Andrea Maria Cappellano, Antonio Sérgio Petrilli, Ana Marisa Chudzinski-Tavassi, Sérgio Cavalheiro
Background: Ependymoma (EPN), the third most common pediatric brain tumor, is a central nervous system (CNS) malignancy originating from the walls of the ventricular system. Surgical resection followed by radiation therapy has been the primary treatment for most pediatric intracranial EPNs. Despite numerous studies into the prognostic value of histological classification, the extent of surgical resection and adjuvant radiotherapy, there have been relatively few studies into the molecular and cellular biology of EPNs...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29774060/metformin-prevents-nerve-growth-factor-dependent-proliferative-and-proangiogenic-effects-in-epithelial-ovarian-cancer-cells-and-endothelial-cells
#5
Maritza P Garrido, Carolina Vera, Margarita Vega, Andrew F G Quest, Carmen Romero
Background: Epithelial ovarian cancer (EOC) is characterized by exacerbated angiogenesis regulated by proangiogenic and growth factors. Nerve growth factor (NGF) is overexpressed in EOC where it promotes proliferation as well as survival and is considered a proangiogenic factor. Metformin, a drug commonly used in the treatment of diabetes, is attributed to antineoplastic effects, but the underlying mechanisms remain unknown. Given that current therapies yield modest results in EOC patients, the aim of this study was to determine the effects of metformin on NGF-enhanced proliferation of EOC cells and the angiogenic potential of endothelial cells...
2018: Therapeutic Advances in Medical Oncology
https://www.readbyqxmd.com/read/29773615/microbial-diversity-in-commercial-bee-pollen-from-europe-chile-and-mexico-based-on-16s-rrna-gene-amplicon-metagenome-sequencing
#6
Vicente D Moreno Andrade, Carlos Saldaña Gutiérrez, Rosa P Calvillo Medina, Andrés Cruz Hérnandez, Moisés A Vázquez Cruz, Alfonso Torres Ruíz, Sergio Romero Gómez, Miguel A Ramos López, Erika Álvarez-Hidalgo, Silvia B López-Gaytan, Natanahel Salvador Ramírez, George H Jones, Jose Luis Hernandez-Flores, Juan Campos-Guillén
Bee pollen is a highly nutritive natural foodstuff. Because of its use as a comestible, the association of bacteria with bee pollen is commercially and biologically important. We report here the bacterial diversity of seven bee pollen samples (five from Europe, one from Chile, and one from Mexico) based on 16S rRNA gene amplicon metagenome sequencing.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29773348/validation-of-standard-method-en-iso-11290-part-2-for-the-enumeration-of-listeria-monocytogenes-in-food
#7
Patricia Rollier, Bertrand Lombard, Laurent Guillier, Danièle François, Karol Romero, Sylvie Pierru, Laurence Bouhier, Nathalie Gnanou Besse
The reference method for the detection and enumeration of L. monocytogenes in food (Standards EN ISO 11290-1&2) have been validated by inter-laboratory studies in the frame of the Mandate M381 from European Commission to CEN. In this paper, the inter-laboratory studies led in 2013 on 5 matrices (cold-smoked salmon, milk powdered infant food formula, vegetables, environment, and cheese) to validate Standard EN ISO 11290-2 are reported. According to the results obtained, the method of the revised Standard EN ISO 11290-2 can be considered as a good method for the enumeration of L...
May 1, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29773109/recombinant-%C3%AB-defensin-126-promotes-bull-sperm-binding-to-bovine-oviductal-epithelia
#8
A Lyons, F Narciandi, E Donnellan, J Romero-Aguirregomezcorta, C O' Farrelly, P Lonergan, K G Meade, S Fair
Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells invitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126...
May 18, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29772087/environmental-change-and-predator-diversity-drive-alpha-and-beta-diversity-in-freshwater-macro-and-microorganisms
#9
Pablo Augusto P Antiqueira, Owen L Petchey, Viviane Piccin Dos Santos, Valéria Maia de Oliveira, Gustavo Quevedo Romero
Global biodiversity is eroding due to anthropogenic causes such as climate change, habitat loss, and trophic simplification of biological communities. Most studies address only isolated causes within a single group of organisms; however, biological groups of different trophic levels may respond in particular ways to different environmental impacts. Our study used natural microcosms to investigate the predicted individual and interactive effects of warming, changes in top predator diversity, and habitat size on the alpha and beta diversity of macrofauna, microfauna and bacteria...
May 17, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29771683/an-individualized-strategy-to-estimate-the-effect-of-deformable-registration-uncertainty-on-accumulated-dose-in-the-upper-abdomen
#10
Yibing Wang, Steven F Petit, Eliana Vasquez Osorio, Vikas Gupta, Alejandra Méndez Romero, Ben J M Heijmen
In the abdomen, it is challenging to assess the accuracy of deformable image registration (DIR) for individual patients, due to the lack of clear anatomical landmarks, which can hamper clinical applications that require high accuracy DIR, such as adaptive radiotherapy. In this study, we propose and evaluate a methodology for estimating the impact of uncertainties in DIR on calculated accumulated dose in the upper abdomen, in order to aid decision making in adaptive treatment approaches. 
 Sixteen liver metastasis patients treated with SBRT were evaluated...
May 17, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29771353/predictors-for-identifying-burn-sepsis-and-performance-versus-existing-criteria
#11
David M Hill, Matthew D Percy, Sai R Velamuri, Julio Lanfranco, Ivan Romero Legro, Scott E Sinclair, William L Hickerson
Sepsis is the largest cause of mortality in thermally injured patients. Traditional SIRS criteria do not aid diagnosis of sepsis in burn centers. Studies have attempted identification of the best indicators of sepsis in the thermal injured patient, but predictive variables are inconsistent across the various studies. Currently, consensus guidelines lack evidential support as to which patients will benefit most from prompt antimicrobial therapy. The purpose of our study was to evaluate novel diagnostic parameters for thermal injured patients with known sepsis and compare these parameters with existing diagnostic criteria...
May 16, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/29771134/effects-of-the-incorporation-of-cantaloupe-pulp-in-yogurt-physicochemical-phytochemical-and-rheological-properties
#12
F Kermiche, L Boulekbache-Makhlouf, M Félix, L Harkat-Madouri, H Remini, K Madani, A Romero
The therapeutic effects of cantaloupe are of great interest for the development of functional foods such as yogurt. In this study a new dairy product has been formulated by enriching natural yogurt with fruit cantaloupe (yogurt with cantaloupe puree, yogurt with dry cantaloupe and yogurt with dry cantaloupe and cantaloupe puree). Thus, composition (moisture, ash, lipids, proteins), including amino acid contents, lactic flora as well as rheological (viscoelasticity, viscosity) property of cantaloupe yogurt and natural yogurt is assessed...
January 1, 2018: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/29771104/-appropriateness-of-antimicrobial-prescriptions-in-the-emergency-department-of-a-tertiary-hospital
#13
L Yunquera-Romero, I Márquez-Gómez, A Henares-López, M J Morales-Lara, C Gallego Fernández, R Asensi-Díez
OBJECTIVE: Antibiotics are widely prescribed in the Emergency Department (ED), representing 26-62% of outpatient antibiotic prescriptions. Around 40% of antibiotic prescriptions in hospitalized patients are inappropriate or unnecessary. The aim of the study was to assess the appropriateness of antibiotic prescriptions according to local empirical antibiotic treatment guidelines, in the ED of a tertiary hospital. METHODS: Observational, retrospective study including patients attending the ED in November 2016, with an antibiotic prescription, excluding those from residents...
May 16, 2018: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29770693/vine-shoot-tannins-effect-of-post-pruning-storage-and-toasting-treatment
#14
Cristina Cebrían-Tarancon, Rosario Sanchez-Gomez, Sergio Gómez-Alonso, Isidro Hermosin-Gutierrez, Adela Mena-Morales, Esteban García-Romero, M Rosario Salinas, Amaya Zalacain
For first time vine-shoot tannin composition was carried out by means of HPLC-DAD-ESI-MS/MS. Two vine-shoot cultivars (Airén and Cencibel) with different post-pruning storage time and submitted to a toasting process, were assayed. There was no trace of gallotannins nor ellagitannins, but a high proanthocyanidins content and a mean degree of polymerization (mDP) close to 3 was characterized. The higher concentration of proanthocyanidins corresponded to Airén after 6 months post-pruning storage and at 3 months for Cencibel...
May 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29770505/poor-self-reported-sleep-quality-and-health-related-quality-of-life-in-patients-with-chronic-fatigue-syndrome-myalgic-encephalomyelitis
#15
Jesús Castro-Marrero, Maria C Zaragozá, Sergio González-Garcia, Luisa Aliste, Naia Sáez-Francàs, Odile Romero, Alex Ferré, Tomás Fernández de Sevilla, José Alegre
Non-restorative sleep is a hallmark symptom of chronic fatigue syndrome/myalgic encephalomyelitis. However, little is known about self-reported sleep disturbances in these subjects. This study aimed to assess the self-reported sleep quality and its impact on quality of life in a Spanish community-based chronic fatigue syndrome/myalgic encephalomyelitis cohort. A prospective cross-sectional cohort study was conducted in 1,455 Spanish chronic fatigue syndrome/myalgic encephalomyelitis patients. Sleep quality, fatigue, pain, functional capacity impairment, psychopathological status, anxiety/depression and health-related quality of life were assessed using validated subjective measures...
May 16, 2018: Journal of Sleep Research
https://www.readbyqxmd.com/read/29769702/fine-mapping-of-98-obesity-loci-in-mexican-children
#16
Hsin Yen Liu, Akram Alyass, Arkan Abadi, Jesus Peralta-Romero, Fernando Suarez, Jaime Gomez-Zamudio, Astride Audirac, Esteban J Parra, Miguel Cruz, David Meyre
BACKGROUND/OBJECTIVES: Mexico has one of the highest prevalence of childhood obesity in the world. Genome-wide association studies (GWAS) for obesity have identified multiple single-nucleotide polymorphisms (SNPs) in populations of European, East Asian, and African descent. The contribution of these loci to obesity in Mexican children is unclear. We assessed the transferability of 98 obesity loci in Mexican children and fine-mapped the association signals. SUBJECTS/METHODS: The study included 405 and 390 Mexican children with normal weight and obesity...
March 12, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29769519/optical-functionalization-of-human-class-a-orphan-g-protein-coupled-receptors
#17
Maurizio Morri, Inmaculada Sanchez-Romero, Alexandra-Madelaine Tichy, Stephanie Kainrath, Elliot J Gerrard, Priscila P Hirschfeld, Jan Schwarz, Harald Janovjak
G-protein-coupled receptors (GPCRs) form the largest receptor family, relay environmental stimuli to changes in cell behavior and represent prime drug targets. Many GPCRs are classified as orphan receptors because of the limited knowledge on their ligands and coupling to cellular signaling machineries. Here, we engineer a library of 63 chimeric receptors that contain the signaling domains of human orphan and understudied GPCRs functionally linked to the light-sensing domain of rhodopsin. Upon stimulation with visible light, we identify activation of canonical cell signaling pathways, including cAMP-, Ca2+ -, MAPK/ERK-, and Rho-dependent pathways, downstream of the engineered receptors...
May 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29768501/secretion-of-autoimmune-antibodies-in-the-human-subcutaneous-adipose-tissue
#18
Daniela Frasca, Alain Diaz, Maria Romero, Seth Thaller, Bonnie B Blomberg
The adipose tissue (AT) contributes to systemic and B cell intrinsic inflammation, reduced B cell responses and secretion of autoimmune antibodies. In this study we show that adipocytes in the human obese subcutaneous AT (SAT) secrete several pro-inflammatory cytokines and chemokines, which contribute to the establishment and maintenance of local and systemic inflammation, and consequent suboptimal immune responses in obese individuals, as we have previously shown. We also show that pro-inflammatory chemokines recruit immune cells expressing the corresponding receptors to the SAT, where they also contribute to local and systemic inflammation, secreting additional pro-inflammatory mediators...
2018: PloS One
https://www.readbyqxmd.com/read/29768277/the-brief-sexual-symptom-checklist-as-a-screening-tool-for-sex-related-problems-in-the-primary-care-setting-focus-on-erectile-dysfunction
#19
Javier Romero Otero, Borja García Gómez, José Medina Polo, Esther García Rojo, Eduardo García Cruz, Francois Peinado, Raquel Sopeña Sutil, Giorgio Bozzini, Alfredo Rodríguez Antolín
PURPOSE: To assess the performance of the Brief Sexual Symptom Checklist for men (BSSC-M) questionnaire in General Practitioner's (GP) consults in Spain. METHODS: Multicenter, cross-sectional study conducted in Spain among men ≥50 years, visiting a GP for any reason, and being able to answer self-administered questionnaires. Patients receiving medicines for erectile dysfunction (ED) and those with poor functional status were excluded. Sexual satisfaction was assessed by the BSSC-M, ED by the Sexual Health Inventory for Men (SHIM), and quality of life (QoL) using a 5-point Likert scale...
May 16, 2018: Urologia Internationalis
https://www.readbyqxmd.com/read/29767915/meaning-of-parenting-as-a-teenager
#20
Carolina Antolinez Figueroa, Carlos Julio Saavedra Cantor, Ana Helena Puerto Guerrero, Carol Margeth Calderón Romero
OBJECTIVES: Describe the meaning of parenting as a teenager. METHODS: Study of the qualitative approach with phenomenological approach in the fourth village of San Cristobal (Bogotá, Colombia). The information was obtained by means of semi-structed interviews, participant observation, photos, daily and focal groups field. The information analysis was performed using the phenomenological method of Amadeo Giorgi. RESULTS: The meaning of their parenthood in the adolescents revealed five units of sense: emotional ambivalence, paternity as a process of learning, changes in lifestyle, making a life project and support networks...
October 2017: Investigación y Educación en Enfermería
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