keyword
MENU ▼
Read by QxMD icon Read
search

respiratory virus infection

keyword
https://www.readbyqxmd.com/read/29223788/a-multiplex-real-time-rt-pcr-for-simultaneous-detection-of-four-most-common-avian-respiratory-viruses
#1
Nacira Laamiri, Rim Aouini, Boutheina Marnissi, Abdeljelil Ghram, Issam Hmila
A one-step multiplex real-time reverse transcription-PCR (rRT-PCR) assay was developed for simultaneous detection and quantification of four avian respiratory viruses: avian influenza virus (AIV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and infectious laryngotracheitis virus (ILTV). In comparison with the singleplex rRT-PCR, the specificity, the sensitivity and the reproducibility of the new assay were evaluated and validated using 70 clinical samples. The optimal cutoff point, the corresponding limit of quantification (LoQ) and the limit of detection (LoD) were statistical established based on receiver operating characteristic (ROC) curve analysis...
December 7, 2017: Virology
https://www.readbyqxmd.com/read/29222615/knockdown-expression-of-il-10r%C3%AE-gene-inhibits-prrsv-replication-and-elevates-immune-responses-in-pbmcs-of-tibetan-pig-in-vitro
#2
Yihui Chen, Ye Wang, Kai Zeng, Yun-Feng Lei, Xiao-Hui Chen, San-Cheng Ying, Xue-Bin Lv, Zezhou Wang, Rong Gao
Increase of interleukin-10 (IL-10) induced by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection has been intensely studied to inhibit the anti-viral responses of host pigs. Blockade of expression of IL-10 receptor (IL-10R) by RNA interference (RNAi) may relieve the immunosuppression caused by excessive IL-10 in PRRSV infection. The recombinant short hairpin expressing plasmid targeted to pig IL-10Rα was transfected into peripheral blood mononuclear cells of Tibetan pig (Tp-PBMCs) prior to PRRSV inoculation, then the replication of PRRSV and immune responses in Tp-PBMCs were evaluated...
December 8, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29222500/long-term-outcomes-in-survivors-of-epidemic-influenza-a-h7n9-virus-infection
#3
Jiajia Chen, Jie Wu, Shaorui Hao, Meifang Yang, Xiaoqing Lu, Xiaoxiao Chen, Lanjuan Li
Patients who survive influenza A (H7N9) virus infection are at risk of physical and psychological complications of lung injury and multi-organ dysfunction. However, there were no prospectively individualized assessments of physiological, functional and quality-of-life measures after hospital discharge. The current study aims to assess the main determinants of functional disability of these patients during the follow-up. Fifty-six influenza A (H7N9) survivors were investigated during the 2-year after discharge from the hospital...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29220699/hiv-1-tat-protein-induces-dna-damage-in-human-peripheral-blood-b-lymphocytes-via-mitochondrial-ros-production
#4
Rawan El-Amine, Diego Germini, Vlada V Zakharova, Tatyana Tsfasman, Eugene V Sheval, Ruy A N Louzada, Corinne Dupuy, Chrystèle Bilhou-Nabera, Aline Hamade, Fadia Najjar, Eric Oksenhendler, Marс Lipinski, Boris V Chernyak, Yegor S Vassetzky
Human immunodeficiency virus (HIV) infection is associated with B-cell malignancies in patients though HIV-1 is not able to infect B-cells. The rate of B-cell lymphomas in HIV-infected individuals remains high even under the combined antiretroviral therapy (cART) that reconstitutes the immune function. Thus, the contribution of HIV-1 to B-cell oncogenesis remains enigmatic. HIV-1 induces oxidative stress and DNA damage in infected cells via multiple mechanisms, including viral Tat protein. We have detected elevated levels of reactive oxygen species (ROS) and DNA damage in B-cells of HIV-infected individuals...
December 5, 2017: Redox Biology
https://www.readbyqxmd.com/read/29217968/investigation-of-markers-of-allergic-sensitization-and-viral-infections-in-children-with-allergy-and-asthma
#5
Rūta Dubakienė, Vilija Rubinaitė, Malvina Petronytė, Indrė Dalgėdienė, Odilija Rudzevičienė, Dalia Dubakaitė, Palmira Rudalevičienė, Aurelija Žvirblienė
Background: Allergic diseases are the most prevalent chronic diseases in the developed countries. It is believed that early allergic sensitization and respiratory viral infections play an important role in the development of allergic diseases and asthma. Methods: The current study investigated the correlation between asthma, allergy, and various markers - allergen-specific IgE, IgG4 and IgA, ECP, IgM, and IgG antibodies against respiratory viruses hRSV and hPIV1-4 - in blood serum samples from 80 children (mean age 5...
2017: Acta Medica Lituanica
https://www.readbyqxmd.com/read/29217660/chimeric-pneumoviridae-fusion-proteins-as-immunogens-to-induce-cross-neutralizing-antibody-responses
#6
Eduardo Olmedillas, Olga Cano, Isidoro Martínez, Daniel Luque, María C Terrón, Jason S McLellan, José A Melero, Vicente Más
Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV), two members of the Pneumoviridae family, account for the majority of severe lower respiratory tract infections worldwide in very young children. They are also a frequent cause of morbidity and mortality in the elderly and immunocompromised adults. High levels of neutralizing antibodies, mostly directed against the viral fusion (F) glycoprotein, correlate with protection against either hRSV or hMPV However, no cross-neutralization is observed in polyclonal antibody responses raised after virus infection or immunization with purified F proteins...
December 7, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/29215596/rsv-infection-in-human-macrophages-promotes-cxcl10-ip-10-expression-during-bacterial-co-infection
#7
Daniela Machado, Jonathan Hoffmann, Marie Moroso, Manuel Rosa-Calatrava, Hubert Endtz, Olivier Terrier, Glaucia Paranhos-Baccalà
Respiratory syncytial virus (RSV), a major etiologic agent of acute lower respiratory infection constitutes the most important cause of death in young children worldwide. Viral/bacterial mixed infections are related to severity of respiratory inflammatory diseases, but the underlying mechanisms remain poorly understood. We have previously investigated the intracellular mechanisms that mediate the immune response in the context of influenza virus/Streptococcus pneumoniae (Sp) co-infection using a model of human monocyte-derived macrophages (MDMs)...
December 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29215570/host-cell-restriction-factors-that-limit-influenza-a-infection
#8
REVIEW
Fernando Villalón-Letelier, Andrew G Brooks, Philippa M Saunders, Sarah L Londrigan, Patrick C Reading
Viral infection of different cell types induces a unique spectrum of host defence genes, including interferon-stimulated genes (ISGs) and genes encoding other proteins with antiviral potential. Although hundreds of ISGs have been described, the vast majority have not been functionally characterised. Cellular proteins with putative antiviral activity (hereafter referred to as "restriction factors") can target various steps in the virus life-cycle. In the context of influenza virus infection, restriction factors have been described that target virus entry, genomic replication, translation and virus release...
December 7, 2017: Viruses
https://www.readbyqxmd.com/read/29214503/epidemiology-and-clinical-characteristics-of-human-coronaviruses-oc43-229e-nl63-and-hku1-a-study-of-hospitalized-children-with-acute-respiratory-tract-infection-in-guangzhou-china
#9
Zhi-Qi Zeng, De-Hui Chen, Wei-Ping Tan, Shu-Yan Qiu, Duo Xu, Huan-Xi Liang, Mei-Xin Chen, Xiao Li, Zheng-Shi Lin, Wen-Kuan Liu, Rong Zhou
Human coronaviruses (HCoV) OC43, 229E, NL63, and HKU1 are common respiratory viruses which cause various respiratory diseases, including pneumonia. There is a paucity of evidence on the epidemiology and clinical manifestations of these four HCoV strains worldwide. We collected 11,399 throat swabs from hospitalized children with acute respiratory tract infection from July 2009 to June 2016 in Guangzhou, China. These were tested for four strains of HCoV infection using real-time polymerase chain reaction (PCR)...
December 6, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29214495/comparison-of-anti-influenza-virus-activity-and-pharmacokinetics-of-oseltamivir-free-base-and-oseltamivir-phosphate
#10
Jin Soo Shin, Keun Bon Ku, Yejin Jang, Yi-Seul Yoon, Daeho Shin, Oh Seung Kwon, Yun Young Go, Seong Soon Kim, Myoung Ae Bae, Meehyein Kim
Influenza viruses are major human respiratory pathogens that cause high morbidity and mortality worldwide. Currently, prophylactic vaccines and therapeutic antiviral agents are used to prevent and control influenza virus infection. Oseltamivir free base (OSV-FB), a modified generic antiviral drug of Tamiflu (oseltamivir phosphate, OSV-P), was launched in the Republic of Korea last year. Here, we examine the bioequivalence of these two compounds by assessing their antiviral efficacy in infected cells and in a mouse model...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29213258/the-complexity-of-antibody-responses-elicited-against-the-respiratory-syncytial-virus-glycoproteins-in-hospitalized-children-younger-than-2-years
#11
Alfonsina Trento, Rosa Rodríguez-Fernández, María I González-Sánchez, Felipe González-Martínez, Vicente Mas, Mónica Vázquez, Concepción Palomo, José A Melero
The influence of age and maternal antibodies on the antibody responses to human respiratory syncytial virus (hRSV) glycoproteins in very young children has been a matter of controversy. Both, immaturity of the immune system at very early age and suppression of the host immune response by high level of maternal antibodies have been claimed to limit the host antibody response to virus infection and to jeopardize the use of hRSV vaccines under development in that age group. Hence, the antibody responses to the two major hRSV glycoproteins (F and G) were evaluated in children younger than 2 years, hospitalized with laboratory confirmed hRSV bronchiolitis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29212934/influenza-a-virus-reassortment-is-limited-by-anatomical-compartmentalization-following-co-infection-via-distinct-routes
#12
Mathilde Richard, Sander Herfst, Hui Tao, Nathan T Jacobs, Anice C Lowen
Exchange of gene segments through reassortment is a major feature of influenza A virus evolution and frequently contributes to the emergence of novel epidemic, pandemic and zoonotic strains. It has long been evident that viral diversification through reassortment is constrained by genetic incompatibility between divergent parental viruses. In contrast, the role of virus-extrinsic factors in determining the likelihood of reassortment has remained unclear. To evaluate the impact of such factors in the absence of confounding effects of segment mismatch, we previously reported an approach in which reassortment between wild-type (wt) and genetically tagged variant (var) viruses of the same strain is measured...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29212926/syrian-hamster-as-an-animal-model-for-the-study-of-human-influenza-virus-infection
#13
Kiyoko Iwatsuki-Horimoto, Noriko Nakajima, Yurie Ichiko, Yuko Sakai-Tagawa, Takeshi Noda, Hideki Hasegawa, Yoshihiro Kawaoka
Ferrets and mice are frequently used as animal models for influenza research. However, ferrets are demanding in terms of housing space and handling, whereas mice are not naturally susceptible to infection with human influenza A or B viruses. Therefore, prior adaptation of human viruses is required for their use in mice. In addition, there are no mouse-adapted variants of the recent H3N2 viruses, because these viruses do not replicate well in mice. In this study, we investigated the susceptibility of Syrian hamsters to influenza viruses with a view to using them as an alternative animal model to mice...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29212531/etiology-of-respiratory-tract-infections-in-the-community-and-clinic-in-ilorin-nigeria
#14
Olatunji Kolawole, Michael Oguntoye, Tina Dam, Rumi Chunara
OBJECTIVE: Recognizing increasing interest in community disease surveillance globally, the goal of this study was to investigate whether respiratory viruses circulating in the community may be represented through clinical (hospital) surveillance in Nigeria. RESULTS: Children were selected via convenience sampling from communities and a tertiary care center (n = 91) during spring 2017 in Ilorin, Nigeria. Nasal swabs were collected and tested using polymerase chain reaction...
December 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29212450/the-impact-of-virus-infections-on-pneumonia-mortality-is-complex-in-adults-a-prospective-multicentre-observational-study
#15
Naoko Katsurada, Motoi Suzuki, Masahiro Aoshima, Makito Yaegashi, Tomoko Ishifuji, Norichika Asoh, Naohisa Hamashige, Masahiko Abe, Koya Ariyoshi, Konosuke Morimoto
BACKGROUND: Various viruses are known to be associated with pneumonia. However, the impact of viral infections on adult pneumonia mortality remains unclear. This study aimed to clarify the effect of virus infection on pneumonia mortality among adults stratified by virus type and patient comorbidities. METHODS: This multicentre prospective study enrolled pneumonia patients aged ≥15 years from September 2011 to August 2014. Sputum samples were tested by in-house multiplex polymerase chain reaction assays to identify 13 respiratory viruses...
December 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29212291/fecal-respiratory-virus-in-acute-viral-respiratory-infection-and-nasopharyngeal-diarrheal-virus-in-acute-viral-gastroenteritis-clinical-impact-of-ectopic-viruses-is-questionable
#16
Oh Joo Kweon, Yong Kwan Lim, Hye Ryoun Kim, Tae-Hyoung Kim, Mi-Kyung Lee
Our aim was to determine the detection rate of respiratory viruses (RVs) in feces of patients with acute viral respiratory infection (AVRI) and the detection rate of diarrheal viruses (DVs) in nasopharyngeal samples from patients with acute viral gastroenteritis. The relationships between the presence of fecal RVs or nasopharyngeal DVs and their impacts on the clinical severity were also investigated. Total 144 fecal specimens collected from AVRI patients and 95 nasopharyngeal specimens collected from acute viral gastroenteritis patients...
December 8, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29210203/the-effects-of-the-attributable-fraction-and-the-duration-of-symptoms-on-burden-estimates-of-influenza-associated-respiratory-illnesses-in-a-high-hiv-prevalence-setting-south-africa-2013-2015
#17
Stefano Tempia, Sibongile Walaza, Jocelyn Moyes, Adam L Cohen, Claire von Mollendorf, Meredith L McMorrow, Sarona Mhlanga, Florette K Treurnicht, Marietjie Venter, Marthi Pretorius, Orienka Hellferscee, Nicole Wolter, Anne von Gottberg, Arthemon Nguweneza, Johanna M McAnerney, Halima Dawood, Ebrahim Variava, Shabir A Madhi, Cheryl Cohen
BACKGROUND: The attributable fraction of influenza virus detection to illness (INF-AF) and the duration of symptoms as a surveillance inclusion criterion could potentially have substantial effects on influenza disease burden estimates. METHODS: We estimated rates of influenza-associated influenza-like illness (ILI) and severe acute (SARI-10) or chronic (SCRI-10) respiratory illness (using a symptom duration cut-off of ≤10 days) among HIV-infected and HIV-uninfected patients attending 3 hospitals and 2 affiliated clinics in South Africa during 2013-2015...
December 6, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29209590/risk-factors-for-community-acquired-pneumonia-among-adults-in-kenya-a-case-control-study
#18
Esther Muthumbi, Brett S Lowe, Cyprian Muyodi, Esther Getambu, Fergus Gleeson, J Anthony G Scott
Background: Pneumonia is a leading cause of morbidity and mortality among adults worldwide; however, the risk factors for community-acquired pneumonia in Africa are not well characterized. Methods: The authors recruited 281 cases of community-acquired pneumonia and 1202 hospital controls among patients aged ≥15 years who attended Kilifi District Hospital/Coast Provincial General Hospital in Kenya between 1994 and 6. Cases were admissions with an acute illness with ≥2 respiratory signs and evidence of consolidation on a chest radiograph...
2017: Pneumonia
https://www.readbyqxmd.com/read/29209121/fatal-gastrointestinal-histoplasmosis-15-years-after-orthotopic-liver-transplantation
#19
Nikita Agrawal, David Ej Jones, Jessica K Dyson, Tim Hoare, Sharon A Melmore, Stephanie Needham, Nick P Thompson
We report a case of ileo-colonic Histoplasmosis without apparent respiratory involvement in a patient who had previously undergone an orthotopic liver transplant (OLT) for primary biliary cholangitis 15 years earlier. The recipient lived in the United Kingdom, a non-endemic region for Histoplasmosis. However, she had previously lived in rural southern Africa prior to her OLT. The patient presented with iron deficiency anaemia, diarrhoea, abdominal pain and progressive weight loss. She reported no previous foreign travel, however, it later became known that following her OLT she had been on holiday to rural southern Africa...
November 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29208560/multiplex-pcr-system-for-the-rapid-diagnosis-of-respiratory-virus-infection-a-systematic-review-and-meta-analysis
#20
REVIEW
Huai-Shiuan Huang, Chi-Lin Tsai, Jeffrey Chang, Tzu-Chun Hsu, Samuel Lin, Chien-Chang Lee
OBJECTIVES: We aim to provide a summary of evidence for the diagnostic accuracies of three multiplex PCR systems (mPCRs): BioFire Filmarray RP (Filmarray), Nanosphere Verigene RV+ test (Verigene) and Hologic Gen-Probe Prodesse assays on the detection of viral respiratory infections. METHODS: A comprehensive search up to July 1st, 2017 was conducted on Medline and Embase for studies that utilized Filmarray, Verigene and Prodesse for diagnosis of viral respiratory infections...
December 2, 2017: Clinical Microbiology and Infection
keyword
keyword
35467
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"