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https://www.readbyqxmd.com/read/29150953/new-algorithm-for-simulation-of-proteoglycan-loss-and-collagen-degeneration-in-the-knee-joint-data-from-the-osteoarthritis-initiative
#1
Mika E Mononen, Petri Tanska, Hanna Isaksson, Rami K Korhonen
Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic. Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network. Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow and collagen fibril network) occurring in osteoarthritis. While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability...
November 18, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29149794/love-intimacy-and-sexuality-in-residential-dementia-care-a-spousal-perspective
#2
Tineke Sm Roelofs, Katrien G Luijkx, Petri Jcm Embregts
The experiences and needs of spouses of residential care facility residents with dementia, regarding friendship, love, intimacy, and sexuality were explored. Understanding of how spouses make sense of their experiences was pursued. Semi-structured interviews were held with nine spouses of people with dementia, living in high intensive 24-hour care units within residential care facilities. The results show that friendship, love, intimacy, and sexuality were still embedded in the couples' marital lives, but all in their own way...
January 1, 2017: Dementia
https://www.readbyqxmd.com/read/29148231/employment-status-at-time-of-first-hospitalization-for-heart-failure-is-associated-with-a-higher-risk-of-death-and-rehospitalization-for-heart-failure
#3
Rasmus Rørth, Emil L Fosbøl, Ulrik M Mogensen, Kristian Kragholm, Anna-Karin Numé, Gunnar H Gislason, Pardeep S Jhund, Mark C Petrie, John J V McMurray, Christian Torp-Pedersen, Lars Køber, Søren L Kristensen
AIMS: Employment status at time of first heart failure (HF) hospitalization may be an indicator of both self-perceived and objective health status. In this study, we examined the association between employment status and the risk of all-cause mortality and recurrent HF hospitalization in a nationwide cohort of patients with HF. METHODS AND RESULTS: We identified all patients of working age (18-60 years) with a first HF hospitalization in the period 1997-2015 in Denmark, categorized according to whether or not they were part of the workforce at time of the index admission...
November 16, 2017: European Journal of Heart Failure
https://www.readbyqxmd.com/read/29148165/dinitroaniline-herbicide-resistance-in-a-multiple-resistant-lolium-rigidum-population
#4
Jinyi Chen, Qin Yu, Mechelle Owen, Heping Han, Stephen Powles
BACKGROUND: The pre-emergence dinitroaniline herbicides (such as trifluralin and pendimethalin) are vital to Australian no-till farming systems. A Lolium rigidum population collected from the Western Australian grain belt with a 12-year trifluralin use history was characterised for resistance to dinitroaniline, acetyl CoA carboxylase (ACCase)- and acetolactate synthase (ALS)-inhibiting herbicides. Target-site resistance mechanisms were investigated. RESULTS: This L...
November 16, 2017: Pest Management Science
https://www.readbyqxmd.com/read/29147538/scaling-behaviour-and-rate-determining-steps-in-filamentous-self-assembly
#5
Georg Meisl, Luke Rajah, Samuel A I Cohen, Manuela Pfammatter, Anđela Šarić, Erik Hellstrand, Alexander K Buell, Adriano Aguzzi, Sara Linse, Michele Vendruscolo, Christopher M Dobson, Tuomas P J Knowles
The formation of filaments from naturally occurring protein molecules is a process at the core of a range of functional and aberrant biological phenomena, such as the assembly of the cytoskeleton or the appearance of aggregates in Alzheimer's disease. The macroscopic behaviour associated with such processes is remarkably diverse, ranging from simple nucleated growth to highly cooperative processes with a well-defined lagtime. Thus, conventionally, different molecular mechanisms have been used to explain the self-assembly of different proteins...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29147003/real-time-wash-free-detection-of-unlabeled-pna-dna-hybridization-using-discrete-fet-sensor
#6
Matti Kaisti, Anssi Kerko, Eero Aarikka, Petri Saviranta, Zhanna Boeva, Tero Soukka, Ari Lehmusvuori
We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA backbone. Similar detection schemes have mainly concentrated on sensitivity improvement with an emphasis on new sensor structures. Our approach focuses on using an extended-gate that separates the FET and the sensing electrode yielding a simple and mass fabricable device...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146600/n-glycan-profile-and-kidney-disease-in-type-1-diabetes
#7
Mairead L Bermingham, Marco Colombo, Stuart J McGurnaghan, Luke A K Blackbourn, Frano Vučković, Maja Pučić Baković, Irena Trbojević-Akmačić, Gordan Lauc, Felix Agakov, Anna S Agakova, Caroline Hayward, Lucija Klarić, Colin N A Palmer, John R Petrie, John Chalmers, Andrew Collier, Fiona Green, Robert S Lindsay, Sandra Macrury, John A McKnight, Alan W Patrick, Sandeep Thekkepat, Olga Gornik, Paul M McKeigue, Helen M Colhoun
OBJECTIVE: Poorer glycemic control in type 1 diabetes may alter N-glycosylation patterns on circulating glycoproteins, and these alterations may be linked with diabetic kidney disease (DKD). We investigated associations between N-glycans and glycemic control and renal function in type 1 diabetes. RESEARCH DESIGN AND METHODS: Using serum samples from 818 adults who were considered to have extreme annual loss in estimated glomerular filtration rate (eGFR; i.e., slope) based on retrospective clinical records, from among 6,127 adults in the Scottish Diabetes Research Network Type 1 Bioresource Study, we measured total and IgG-specific N-glycan profiles...
November 16, 2017: Diabetes Care
https://www.readbyqxmd.com/read/29145819/recurrent-tuberculosis-in-finland-1995-2013-a-clinical-and-epidemiological-cohort-study
#8
Virve Korhonen, Hanna Soini, Tuula Vasankari, Jukka Ollgren, Pieter W Smit, Petri Ruutu
BACKGROUND: We investigated the epidemiology and prevalence of potential risk factors of tuberculosis (TB) recurrence in a population-based registry cohort of 8084 TB cases between 1995 and 2013. METHODS: An episode of recurrent TB was defined as a case re-registered in the National Infectious Disease Register at least 360 days from the date of the initial registration. A regression model was used to estimate risk factors for recurrence in the national cohort...
November 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29145765/epidemiological-and-treatment-related-factors-contribute-to-improved-outcome-of-oropharyngeal-squamous-cell-carcinoma-in-finland
#9
Lauri Jouhi, Elina Halme, Heikki Irjala, Kauko Saarilahti, Petri Koivunen, Matti Pukkila, Jaana Hagström, Caj Haglund, Paula Lindholm, Pasi Hirvikoski, Samuli Vaittinen, Anna Ellonen, Jukka Tikanto, Henry Blomster, Jussi Laranne, Reidar Grénman, Antti Mäkitie, Timo Atula
BACKGROUND: Treatment for oropharyngeal squamous cell carcinoma (OPSCC) has changed, as the proportion of human papilloma virus (HPV)-related disease has increased. We evaluated nationwide information on its management and outcome during the treatment paradigm change period. METHODS: We included all patients diagnosed and treated for OPSCC at the five Finnish university hospitals from 2000 to 2009. Patient records and pathology registries provided the clinicopathological data...
November 17, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/29145019/ordered-cylindrical-micropatterned-petri-dishes-used-as-scaffolds-for-cell-growth
#10
Van-Tien Bui, Le Thi Thuy, Joon Sig Choi, Ho-Suk Choi
Three-dimensional (3D) culture dish patterned with a microwell structure demonstrates a great application potential in biotechnology. This study reports on the easy fabrication of an ordered customizable honeycomb microwell array on the surface of polymer substrates including the commercial Petri dish to create a biological platform for cell culture. The fabrication method is based on a very simple solvent dip-coating technique and the methanol accumulation-induced phase separation in which a binary mixture of chloroform and methanol is used to induce a ternary solution and to guarantee the formation of the ordered pore array on the substrate...
November 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29143465/migratory-birds-in-southern-brazil-are-a-source-of-multiple-avian-influenza-virus-subtypes
#11
Jansen de Araujo, Maria Virgínia Petry, Thomas Fabrizio, David Walker, Tatiana Ometto, Luciano M Thomazelli, Angelo L Scherer, Patricia P Serafini, Isaac S Neto, Scott Krauss, Robert G Webster, Richard J Webby, Edison L Durigon
BACKGROUND: There is insufficient knowledge about the relation of avian influenza virus (AIV) to migratory birds in South America. Accordingly, we studied samples obtained over a 4-year period (2009-2012) from wild birds at a major wintering site in Southern Brazil. METHODS: We obtained 1212 oropharyngeal/cloacal samples from wild birds at Lagoa do Peixe National Park and screened them for influenza A virus by RT-PCR amplification of the matrix gene. Virus isolates were subjected to genomic sequencing and antigenic characterization...
November 15, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29142012/a-transcatheter-interatrial-shunt-device-for-the-treatment-of-heart-failure-with-preserved-ejection-fraction-reduce-lap-hf-i-a-phase-2-randomized-sham-controlled-trial
#12
Ted Feldman, Laura Mauri, Rami Kahwash, Sheldon Litwin, Mark J Ricciardi, Pim van der Harst, Martin Penicka, Peter S Fail, David M Kaye, Mark C Petrie, Anupam Basuray, Scott L Hummel, Rhondalyn Forde-McLean, Christopher D Nielsen, Scott Lilly, Joseph M Massaro, Daniel Burkhoff, Sanjiv J Shah
Background -In non-randomized, open-label studies, a transcatheter interatrial shunt device (IASD, Corvia Medical) was associated with lower pulmonary capillary wedge pressure (PCWP), less symptoms, and greater quality of life and exercise capacity in patients with heart failure (HF) and mid-range or preserved ejection fraction (EF ≥ 40%). We conducted the first randomized, sham-controlled trial to evaluate the IASD in HF with EF ≥ 40%. Methods -REDUCE LAP-HF I was a phase 2, randomized, parallel-group, blinded multicenter trial in patients with New York Heart Association (NYHA) class III or ambulatory class IV HF, EF ≥ 40%, exercise PCWP ≥ 25 mmHg, and PCWP-right atrial pressure gradient ≥ 5 mmHg...
November 15, 2017: Circulation
https://www.readbyqxmd.com/read/29137641/improving-e-coli-growth-performance-by-manipulating-small-rna-expression
#13
REVIEW
Alejandro Negrete, Joseph Shiloach
Efficient growth of E. coli, especially for production of recombinant proteins, has been a challenge for the biotechnological industry since the early 1970s. By employing multiple approaches, such as different media composition, various growth strategies and specific genetic manipulations, it is now possible to grow bacteria to concentrations exceeding 100 g/L and to achieve high concentrations of recombinant proteins. Although the growth conditions are carefully monitored and maintained, it is likely that during the growth process cells are exposed to periodic stress conditions, created by fluctuations in pH, dissolved oxygen, temperature, glucose, and salt concentration...
November 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29136882/growth-of-escherichia-coli-on-the-gaas-001-surface
#14
Elnaz Nazemi, Walid M Hassen, Eric H Frost, Jan J Dubowski
Detection of pathogenic bacteria and monitoring their susceptibility to antibiotics are of great importance in the fields of medicine, pharmaceutical research, as well as water and food industries. In order to develop a photonic biosensor for detection of bacteria by taking advantage of photoluminescence (PL) of GaAs-based devices, we have investigated the capture and growth of Escherichia coli K12 on bare and biofunctionalized surfaces of GaAs (001) - a material of interest for capping different semiconductor microstructures...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29133606/effects-of-bariatric-surgery-in-obese-patients-with-hypertension-the-gateway-randomized-trial-gastric-bypass-to-treat-obese-patients-with-steady-hypertension
#15
Carlos Aurelio Schiavon, Angela Cristine Bersch-Ferreira, Eliana Vieira Santucci, Juliana Dantas Oliveira, Camila Ragne Torreglosa, Priscila Torres Bueno, Julia Caldas Frayha, Renato Nakagawa Santos, Lucas Petri Damian, Patricia Malvina Noujaim, Helio Halpern, Frederico L J Monteiro, Ricardo Vitor Cohen, Carlos H Uchoa, Marcio Gonçalves de Souza, Celso Amodeo, Luiz Bortolotto, Dimas Ikeoka, Luciano F Drager, Alexandre Biasi Cavalcanti, Otavio Berwanger
BACKGROUND : Recent research efforts on bariatric surgery have focused on metabolic and diabetes mellitus resolution. Randomized trials designed to assess the impact of bariatric surgery in patients with obesity and hypertension are needed. METHODS : In this randomized, single-center, nonblinded trial, we included patients with hypertension (using ≥2 medications at maximum doses or >2 at moderate doses) and a body mass index between 30.0 and 39.9 kg/m(2) Patients were randomized to Roux-en-Y gastric bypass plus medical therapy or medical therapy alone...
November 13, 2017: Circulation
https://www.readbyqxmd.com/read/29133187/duddingtonia-flagrans-formulated-in-rice-bran-in-the-control-of-oesophagostomum-spp-intestinal-parasite-of-swine
#16
João Victor Facchini Rodrigues, Fabio Ribeiro Braga, Artur Kanadani Campos, Lorendane Millena de Carvalho, Juliana Milani Araujo, Anderson Rocha Aguiar, Carolina Magri Ferraz, Wendeo Ferreira da Silveira, Marisa Caixetta Valadão, Thais de Oliveira, Samuel Galvão de Freitas, Jackson Victor de Araújo
Three experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation...
November 10, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29132810/medium-retaining-petri-dish-insert-to-grow-and-image-cultured-cells
#17
Lech Kiedrowski, Alan Feinerman
BACKGROUND: Microscope chambers that accept glass coverslips with cultured cells are often used to monitor intracellular Ca(2+) concentration ([Ca(2+)]i) during cell superfusion. Unfortunately, the experimental maneuvers associated with the coverslip installation in these chambers (medium removal and re-application) trigger unintended [Ca(2+)]i elevations. NEW METHOD: To prevent these [Ca(2+)]i elevations, a Petri dish insert has been constructed. The insert features a superfusion-optimized well to grow cell cultures...
November 10, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29130467/-a-comparative-study-of-three-systems-for-quantifying-the-spike-and-wave-index-in-patients-with-continuous-spikes-and-waves-during-slow-sleep
#18
G Azcona, I G Gurtubay, A Mosquera, B Ibanez, K Cambra, S Aguilera-Albesa, M E Yoldi-Petri
INTRODUCTION: Continuous spikes and waves during slow sleep (CSWS) is an epileptic encephalopathy of childhood with a pattern of epileptiform discharges during sleep, which, if prolonged over time, produce severe neuropsychological impairment. Quantification of the paroxysms by means of a spike and wave index (SWI) > 85% establishes a presumptive diagnosis and allows early therapy to be started to prevent such impairment. AIMS: To compare the results of the classic method for calculating the SWI against two proposals that optimise the relation between the analysis time employed and the diagnostic sensitivity...
November 16, 2017: Revista de Neurologia
https://www.readbyqxmd.com/read/29130456/strain-coupling-of-oxygen-non-stoichiometry-in-perovskite-thin-films
#19
Andreas Herklotz, Dongkyu Lee, Er-Jia Guo, Tricia L Meyer, Jonathan R Petrie, Ho Nyung Lee
The effects of strain and oxygen vacancies on perovskite thin films have been studied in great detail over the past decades and have been treated separately from each other. While epitaxial strain has been realized as a tuning knob to tailor the functional properties of correlated oxides, oxygen vacancies are usually regarded as undesirable and detrimental. In transition metal oxides, oxygen defects strongly modify the properties and functionalities via changes in oxidation states of the transition metals. However, such coupling is not well understood in epitaxial films, but rather deemed as cumbersome or experimental artifact...
November 13, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29129191/alcohol-use-disorders-are-associated-with-increased-hiv-risk-behaviors-in-cocaine-dependent-methadone-patients
#20
Steven E Meredith, Carla J Rash, Nancy M Petry
People who inject drugs (PWID) are at increased risk of HIV infection. Although methadone maintenance therapy can help lower this risk, many methadone patients continue to engage in HIV risk behaviors, especially patients who use cocaine and alcohol. The purpose of the current study was to investigate relations between alcohol use disorders and HIV risk behavior in 239 cocaine-dependent methadone patients participating in a randomized controlled trial of a behavioral intervention to promote cocaine abstinence...
December 2017: Journal of Substance Abuse Treatment
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