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https://www.readbyqxmd.com/read/28226242/p-falciparum-ligand-binding-to-erythrocytes-induce-alterations-in-deformability-essential-for-invasion
#1
Xavier Sisquella, Thomas Nebl, Jennifer K Thompson, Lachlan Whitehead, Brian M Malpede, Nichole D Salinas, Kelly Rogers, Niraj H Tolia, Andrea Fleig, Joseph O'Neill, Wai-Hong Tham, F David Horgen, Alan F Cowman
The most lethal form of malaria in humans is caused by Plasmodium falciparum. These parasites invade erythrocytes, a complex process involving multiple ligand-receptor interactions. The parasite makes initial contact with the erythrocyte followed by dramatic deformations linked to the function of the Erythrocyte binding antigen family and P. falciparum reticulocyte binding-like families. We show EBA-175 mediates substantial changes in deformability of erythrocytes by binding to glycophorin A and activating a phosphorylation cascade that includes erythrocyte cytoskeletal proteins resulting in changes in the viscoelastic properties of the host cell...
February 22, 2017: ELife
https://www.readbyqxmd.com/read/28222911/store-operated-ca-2-entry-is-not-required-for-fertilization-induced-ca-2-signaling-in-mouse-eggs
#2
Miranda L Bernhardt, Elizabeth Padilla-Banks, Paula Stein, Yingpei Zhang, Carmen J Williams
Repetitive oscillations in cytoplasmic Ca(2+) due to periodic Ca(2+) release from the endoplasmic reticulum (ER) drive mammalian embryo development following fertilization. Influx of extracellular Ca(2+) to support the refilling of ER stores is required for sustained Ca(2+) oscillations, but the mechanisms underlying this Ca(2+) influx are controversial. Although store-operated Ca(2+) entry (SOCE) is an appealing candidate mechanism, several groups have arrived at contradictory conclusions regarding the importance of SOCE in oocytes and eggs...
February 11, 2017: Cell Calcium
https://www.readbyqxmd.com/read/28220887/mass-spectrometric-analysis-of-trpm6-and-trpm7-phosphorylation-reveals-regulatory-mechanisms-of-the-channel-kinases
#3
Na Cai, Zhiyong Bai, Vikas Nanda, Loren W Runnels
TRPM7 and TRPM6 were the first identified bifunctional channels to contain their own kinase domains, but how these channel-kinases are regulated is poorly understood. Previous studies identified numerous phosphorylation sites on TRPM7, but very little is known about TRPM6 phosphorylation or sites on TRPM7 transphosphorylated by TRPM6. Our mass spectrometric analysis of homomeric and heteromeric TRPM7 and TRPM6 channels identified phosphorylation sites on both proteins, as well as several prominent sites on TRPM7 that are commonly modified through autophosphorylation and transphosphorylation by TRPM6...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28185128/effects-of-trpm7-mir-34a-gene-silencing-on-spatial-cognitive-function-and-hippocampal-neurogenesis-in-mice-with-type-1-diabetes-mellitus
#4
Qing-Jiu Zhang, Jie Li, Song-Yun Zhang
This study aimed to explore the effects of transient receptor potential melastatin 7 (TRPM7)/microRNA-34a (miR-34a) gene silencing on spatial cognitive function and hippocampal neurogenesis in mice with type 1 diabetes mellitus (T1DM). BALB/c mice were chosen to establish T1DM models and divided into five groups respectively: the negative control (NC), T1DM, T1DM + si-TRPM7, T1DM + si-miR-34a, and T1DM + si-TRPM7 + si-miR-34a groups. Morris water maze (MWM) test was adopted to observe behavioral alterations in mice...
February 9, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28161478/identification-of-tg100-115-as-a-new-and-potent-trpm7-kinase-inhibitor-which-suppresses-breast-cancer-cell-migration-and-invasion
#5
Chiman Song, Yeonju Bae, JinJoo Jun, Hyomin Lee, Nam Doo Kim, Kyung-Bok Lee, Wooyoung Hur, Jae-Yong Park, Taebo Sim
BACKGROUND: Transient receptor potential melastatin 7 (TRPM7) regulates breast cancer cell proliferation, migration, invasion and metastasis in its ion channel- and kinase domain-dependent manner. The pharmacological effects of TRPM7 ion channel inhibitors on breast cancer cells have been studied, but little is known about the effects of TRPM7 kinase domain inhibitors due to lack of potent TRPM7 kinase inhibitors. METHODS: Screening was performed by using TRPM7 kinase assay...
February 1, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28134400/the-influence-of-phosphate-calcium-and-magnesium-on-matrix-gla-protein-and-vascular-calcification-a-systematic-review
#6
E Houben, A Neradova, L J Schurgers, Marc Vervloet
Vitamin K-dependent matrix Gla protein (MGP) is a key inhibitor of vascular calcification (VC). MGP is synthesized by chondrocytes and vascular smooth muscle cells (VSMC) and the absence or inactivity of MGP results in excessive calcification of both growth plate and vasculature. Apart from its vitamin K dependency little is known about other factors that influence MGP metabolism. Phosphate, calcium and magnesium are involved in bone mineralization and play an important role in VC. In this review we provide a summary of the effect of phosphate, calcium, and magnesium on MGP metabolism...
November 2016: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/28130783/the-trpm7-channel-kinase-regulates-store-operated-calcium-entry
#7
Malika Faouzi, Tatiana Kilch, F David Horgen, Andrea Fleig, Reinhold Penner
The Transient receptor potential melastatin 7 (TRPM7) is a protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins through its kinase domain. In this study we present Store-Operated Calcium Entry (SOCE) as a novel target of TRPM7 kinase activity. TRPM7-deficient chicken DT40 B lymphocytes exhibit a strongly impaired SOCE compared to wild type cells due to reduced Calcium Release Activated Calcium (CRAC) currents and independent of potassium channel regulation, membrane potential changes or changes in cell-cycle distribution...
January 28, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28130109/hypotonicity-activates-a-voltage-dependent-membrane-conductance-in-n2a-neuroblastoma-cells
#8
Akiyuki Taruno, Yoshinori Marunaka
To maintain cellular and bodily homeostasis, cells respond to extracellular stimuli including osmotic stress by activating various ion channels, which have been implicated in many physiological and pathophysiological conditions. However, cellular osmosensory mechanisms remain elusive. Here, we report a novel voltage-dependent current in N2a cells activated by exposure to hypotonic stress. After a hypotonic challenge, N2a cells sequentially develop two distinct currents. The volume-regulated anion channel (VRAC) current emerges first and, after a delay, activation of a previously uncharacterized strongly outwardly rectifying current follows...
January 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28129376/modulation-of-human-cardiac-trpm7-current-by-extracellular-acidic-ph-depends-upon-extracellular-concentrations-of-divalent-cations
#9
Regina Mačianskienė, Mantė Almanaitytė, Aistė Jekabsone, Kanigula Mubagwa
TRPM7 channels participate in a variety of physiological/pathological processes. TRPM7 currents are modulated by protons but opposing effects of external pH (pHo) (potentiation vs inhibition) have been reported. TRPM7 has been less studied in human cardiomyocytes than in heart-derived non-cardiomyocyte cells. We used the whole-cell patch-clamp technique on isolated human atrial cardiomyocytes to investigate the impact of an acidic pHo on the TRPM7 current. With voltage-dependent and other ion channels inhibited, cardiomyocytes were challenged with external acidification in either the presence or the absence of extracellular divalent cations...
2017: PloS One
https://www.readbyqxmd.com/read/28123510/effects-of-prunus-mume-siebold-zucc-in-the-pacemaking-activity-of-interstitial-cells-of-cajal-in-murine-small-intestine
#10
Sang Weon Lee, Sung Jin Kim, Hyungwoo Kim, Dongki Yang, Hyun Jung Kim, Byung Joo Kim
Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28123180/magnesium-prevents-phosphate-induced-vascular-calcification-via-trpm7-and-pit-1-in-an-aortic-tissue-culture-model
#11
Tomohiro Sonou, Masaki Ohya, Mitsuru Yashiro, Asuka Masumoto, Yuri Nakashima, Teppei Ito, Toru Mima, Shigeo Negi, Hiromi Kimura-Suda, Takashi Shigematsu
Previous clinical and experimental studies have indicated that magnesium may prevent vascular calcification (VC), but mechanistic characterization has not been reported. This study investigated the influence of increasing magnesium concentrations on VC in a rat aortic tissue culture model. Aortic segments from male Sprague-Dawley rats were incubated in serum-supplemented high-phosphate medium for 10 days. The magnesium concentration in this medium was increased to demonstrate its role in preventing VC, which was assessed by imaging and spectroscopy...
January 26, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28101241/potential-role-of-melastatin-related-transient-receptor-potential-cation-channel-subfamily-m-gene-expression-in-the-pathogenesis-of-urinary-bladder-cancer
#12
Gülay Güleç Ceylan, Ebru Etem Önalan, Tuncay Kuloğlu, Gülten Aydoğ, İbrahim Keleş, Şenol Tonyali, Cavit Ceylan
Urinary bladder cancer is one of the most common malignancies of the urinary tract. Ion channels and calcium homeostasis are involved in almost all basic cellular mechanisms. The transient receptor potential cation channel subfamily M (TRPM) takes its name from the melastatin protein, which is classified as potential tumor suppressor. To the best of our knowledge, there have been no previous studies in the literature investigating the role of these ion channels in bladder cancer. The present study aimed to determine whether bladder cancer is associated with mRNA expression levels of TRPM ion channel genes, and whether there is the potential to conduct further studies to establish novel treatment modalities...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28094304/the-different-expression-of-trpm7-and-magt1-impacts-on-the-proliferation-of-colon-carcinoma-cells-sensitive-or-resistant-to-doxorubicin
#13
Alessandra Cazzaniga, Claudia Moscheni, Valentina Trapani, Federica I Wolf, Giovanna Farruggia, Azzurra Sargenti, Stefano Iotti, Jeanette A M Maier, Sara Castiglioni
The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive to doxorubicin with their resistant counterpart. We found that resistant cells are growth retarded, and show staminal and ultrastructural features profoundly different from sensitive cells. The resistant phenotype is accompanied by the upregulation of the magnesium transporter MagT1 and the downregulation of the ion channel kinase TRPM7...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091657/zinc-transport-and-the-inhibition-of-the-l-type-calcium-channel-are-two-separable-functions-of-znt-1
#14
Eden Shusterman, Ofer Beharier, Shiri Levy, Raz Zarivach, Yoram Etzion, Craig R Campbell, Il-Ha Lee, Anuwat Dinudom, David I Cook, Asher Peretz, Amos Katz, Daniel Gitler, Arie Moran
Traditionally, proteins are considered to perform a single role, be it as an enzyme, a channel, a transporter or as a structural scaffold. However, recent studies have described moonlighting proteins that perform distinct and independent functions; for example, TRPM7 is both an ion channel and a kinase. ZnT-1 is a member of the Carrier Diffusion Facilitator family that is expressed throughout the phylogenetic tree from bacteria to humans. Since its cloning in 1995, ZnT-1 is considered a major extruder of Zn(2+) based on its capability to protect cells against zinc toxicity...
January 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28061441/activation-of-trpm7-by-naltriben-enhances-migration-and-invasion-of-glioblastoma-cells
#15
Raymond Wong, Ekaterina Turlova, Zhong-Ping Feng, James T Rutka, Hong-Shuo Sun
Glioblastoma (GBM), the most common and aggressive brain tumor in the central nervous system, remains a lethal diagnosis with a median survival of < 15 months. Aberrant expression of the TRPM7 channel has been linked to GBM functions. In this study, using the human GBM cell line U87, we evaluated the TRPM7 activator naltriben on GBM viability, migration, and invasiveness. First, using the whole-cell patch-clamp technique, we showed that naltriben enhanced the endogenous TRPM7-like current in U87 cells. In addition, with Fura-2 Ca2+ imaging, we observed robust Ca2+ influx following naltriben application...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/27988371/fine-mapping-of-qt-regions-in-global-populations-refines-previously-identified-qt-loci-and-identifies-signals-unique-to-african-and-hispanic-descent-populations
#16
Christy L Avery, Christina L Wassel, Melissa A Richard, Heather M Highland, Stephanie Bien, Niha Zubair, Elsayed Z Soliman, Myriam Fornage, Suzette J Bielinski, Ran Tao, Amanda A Seyerle, Sanjiv J Shah, Donald M Lloyd-Jones, Steven Buyske, Jerome I Rotter, Wendy S Post, Stephen S Rich, Lucia A Hindorff, Janina M Jeff, Ralph V Shohet, Nona Sotoodehnia, Dan Yu Lin, Eric A Whitsel, Ulrike Peters, Christopher A Haiman, Dana C Crawford, Charles Kooperberg, Kari E North
BACKGROUND: The electrocardiographically measured QT interval (QT) is heritable and its prolongation is an established risk factor for several cardiovascular DISEASES: Yet, most QT genetic studies have been performed in European ancestral populations, possibly reducing their global relevance. OBJECTIVE: To leverage diversity and improve biologic insight, we fine-mapped 16 of the 35 previously identified QT loci (46%) in populations of African American (n=12,410) and Hispanic/Latino (n=14,837) ancestry...
December 14, 2016: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/27891609/role-of-trpm7-in-cerebral-ischaemia-and-hypoxia
#17
Hong-Shuo Sun
Transient receptor potential melastatin 7 (TRPM7) channel, a calcium-permeable non-selective divalent cation channel, is broadly expressed in various cells and tissues, including the brain. TRPM7 is thought to be coupled to the metabolic state and regulate calcium homeostasis in the cell. TRPM7 takes part in a wide range of cell biology processes that affect cell growth and proliferation, as well as in embryonic development and skeleton formation. TRPM7 plays a significant role in ischaemic and hypoxic brain injury and neuronal cell death...
November 27, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/27818786/trpm7-is-down-regulated-in-both-left-atria-and-left-ventricle-of-ischaemic-cardiomyopathy-patients-and-highly-related-to-changes-in-ventricular-function
#18
Ana Ortega, Esther Roselló-Lletí, Estefanía Tarazón, Carolina Gil-Cayuela, Francisca Lago, Jose-Ramón González-Juanatey, Luis Martinez-Dolz, Manuel Portolés, Miguel Rivera
AIMS: The kinase ion channel transient receptor potential melastatin 7 (TRPM7) is considered a modulator of cardiac fibrosis progression; nevertheless, we lack of studies analysing its role in human ischaemic cardiomyopathy (ICM). Our objective was to analyse the expression of genes encoding cardiac ion channels in human ICM, focusing on the alterations in mRNA levels of TRPM7 and its relationship with changes in the ventricular function. METHODS AND RESULTS: RNA-sequencing was carried out in 13 left ventricular (LV) samples of patients with ICM compared with a control group (n = 10)...
September 2016: ESC Heart Failure
https://www.readbyqxmd.com/read/27803760/carvacrol-alleviates-prostate-cancer-cell-proliferation-migration-and-invasion-through-regulation-of-pi3k-akt-and-mapk-signaling-pathways
#19
Yun Luo, Jie-Ying Wu, Min-Hua Lu, Zhi Shi, Ning Na, Jin-Ming Di
TRPM7 is a potential therapeutic target for treatment of prostate cancer. In this study, we investigated the effects of nonselective TRPM7 inhibitor carvacrol on cell proliferation, migration, and invasion of prostate cancer PC-3 and DU145 cells. Our results showed that carvacrol blocked TRPM7-like currents in PC-3 and DU145 cells and reduced their proliferation, migration, and invasion. Moreover, carvacrol treatment significantly decreased MMP-2, p-Akt, and p-ERK1/2 protein expression and inhibited F-actin reorganization...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27754098/os-21-03-effects-of-the-trpm7-kinase-domain-in-vascular-dysfunction-and-cardiac-fibrosis-induced-by-aldosterone-and-salt
#20
Francisco J Rios, Katie Y Hood, A Karvey, Karla B Neves, Panagiota Anyfanti, Ryszard Nosalski, Livia L Camargo Ll, Augusto C Montezano, Rhian M Touyz
OBJECTIVE: TRPM7 is cation channel with intrinsic kinase activity important for cellular Mg homeostasis. We recently showed that TRPM7-kinase plays a role in aldosterone-mediated vascular effects and inflammation. Here we explored the putative role of TRPM7-kinase in cardiac fibrosis and vascular function in aldosterone-induced hypertension in mice. DESIGN AND METHOD: Wild-type (WT) or heterozygote TRPM7-kinase domain (TRPM7+/-) mice were treated with infused aldosterone (600 μg/Kg/day) and NaCl 1% in drinking water (aldo/salt) for 4 weeks...
September 2016: Journal of Hypertension
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