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https://www.readbyqxmd.com/read/29785067/-in-silico-physicochemical-characterization-and-topology-analysis-of-respiratory-burst-oxidase-homolog-rboh-proteins-from-arabidopsis-and-rice
#1
Gurpreet Kaur, Pratap Kumar Pati
NADPH oxidase (NOX) is a key enzyme involved in the production of apoplastic superoxide (O2-), a type of reactive oxygen species (ROS). Plant Noxes are the homologs of mammalian NADPH oxidase's catalytic subunit and are documented as respiratory burst oxidase homologs (Rbohs). A number of studies have reported their diverse functions in combating various stresses and in plant growth and development. In the present study, a total of 19 Rboh proteins (10 from Arabidopsis thaliana and 9 from Oryza sativa Japonica) were analyzed...
2018: Bioinformation
https://www.readbyqxmd.com/read/29784365/rapid-detection-of-four-mycotoxins-in-corn-using-a-microfluidics-and-microarray-based-immunoassay-system
#2
Yiqi Chen, Xiangrui Meng, Yunzeng Zhu, Minjie Shen, Ying Lu, Jing Cheng, Youchun Xu
Mycotoxins threaten human health seriously because they usually exist in food, fodder and commodities. In this study, a rapid and sensitive immunoassay system for commonly encountered mycotoxins was established based on microfluidics and protein microarrays. Four mycotoxins (T-2 toxin, aflatoxin B1, ochratoxin A, and zearalenone) can be automatically detected in a custom-made microdevice within 30 min under the assistance of a prototype of the instrument with a fluid control system and an imaging system. Once the microdevices are fabricated, they are small-sized and user-friendly...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783231/allergenic-weed-pollen-forecast-under-the-mathematical-modeling-method-implementation-in-ukraine
#3
Irina I Motruk, Michael Yu Antomonov, Victoria V Rodinkova, Olena E Aleksandrova, Oleh V Yermishev
OBJECTIVE: Introduction: Allergies are the most common reason of the chronic diseases in developed countries and represent an important medical, social and economic issue, the relevance of which is growing both in these countries and in Ukraine. The most famous of these allergens group is the pollen of ambrosia and pollen of poaceae, which are ubiquitously distributed in the subtropical and temperate climate. The aim: The objective of our study was to develop the mathematical models, which will be able to indicate the probability of the pollen circulation, and thus these models can simplify the forecast of symptoms risk and improve the prophylaxis of pollinosis...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29783197/quantification-of-21-antihypertensive-drugs-in-serum-using-uhplc-ms-ms
#4
Per Ole M Gundersen, Arne Helland, Olav Spigset, Solfrid Hegstad
BACKGROUND: Poor drug adherence in hypertensive patients can lead to treatment failure and increased cardiovascular morbidity, as well as increased costs to society. An analytical method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MSMS) was developed and validated for use in routine therapeutic drug monitoring (TDM). The method includes 21 antihypertensive drugs or active metabolites from the groups beta blockers (n=5), calcium antagonists (n=5), angiotensin II receptor antagonists (n=4), angiotensin converting enzyme (ACE) inhibitors (n=3) and diuretics (n = 3), in addition to one α1-selective alpha blocker...
April 28, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29783068/reliability-of-the-star-excursion-balance-test-and-two-new-similar-protocols-to-measure-trunk-postural-control
#5
Diego López-Plaza, Casto Juan-Recio, David Barbado, Iñaki Ruiz-Pérez, Francisco J Vera-Garcia
BACKGROUND: Although the Star Excursion Balance test (SEBT) has shown a good intra-session reliability the inter-session reliability of this test has not been deeply studied. Furthermore, there is an evident high influence of the lower-limbs in the performance of the SEBT so even if it has been used to measure core stability it is possibly not the most suitable measurement. OBJECTIVE: The aims of this study were: i) to assess the absolute and relative between-session reliability of the SEBT and two novel variations of this test to assess trunk postural control while sitting, i...
May 18, 2018: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/29782961/baclofen-induced-encephalopathy-in-overdose-modeling-of-the-electroencephalographic-effect-concentration-relationships-and-contribution-of-tolerance-in-the-rat
#6
Magali Chartier, Isabelle Malissin, Salma Tannous, Laurence Labat, Patricia Risède, Bruno Mégarbane, Lucie Chevillard
Baclofen, a γ-amino-butyric acid type-B receptor agonist with exponentially increased use at high-dose to facilitate abstinence in chronic alcoholics, is responsible for increasing poisonings. Baclofen overdose may induce severe encephalopathy and electroencephalographic (EEG) abnormalities. Whether prior prolonged baclofen treatment may influence the severity of baclofen-induced encephalopathy in overdose has not been established. We designed a rat study to characterize baclofen-induced encephalopathy, correlate its severity with plasma concentrations and investigate the contribution of tolerance...
May 18, 2018: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/29782657/phantom-clinical-and-texture-indices-evaluation-and-optimization-of-a-penalized-likelihood-image-reconstruction-method-q-clear-on-a-bgo-pet-ct-scanner
#7
Gabriel Reynés-Llompart, Cristina Gámez-Cenzano, José Luís Vercher-Conejero, Aida Sabaté-Llobera, Nahúm Calvo-Malvar, Josep M Martí-Climent
INTRODUCTION: The aim of this study was to evaluate the behavior of a penalized-likelihood image reconstruction method (Q.Clear) under different count statistics and lesion-to-background ratios (LBR) on a BGO scanner, in order to obtain an optimum penalization factor (β value) to study and optimize for different acquisition protocols and clinical goals. METHODS: Both phantom and patient images were evaluated. Data from an image quality phantom were acquired using different Lesion-to-Background ratios and acquisition times...
May 21, 2018: Medical Physics
https://www.readbyqxmd.com/read/29781934/absolute-reliability-and-concurrent-validity-of-the-stryd-system-for-the-assessment-of-running-stride-kinematics-at-different-velocities
#8
Felipe García-Pinillos, Luis E Roche-Seruendo, Noel Marcén-Cinca, Luis A Marco-Contreras, Pedro A Latorre-Román
García-Pinillos, F, Roche-Seruendo, LE, Marcen-Cinca, N, Marco-Contreras, LA, and Latorre-Román, PA. Absolute reliability and concurrent validity of the Stryd system for the assessment of running stride kinematics at different velocities. J Strength Cond Res XX(X): 000-000, 2018-This study aimed to determine the absolute reliability and to evaluate the concurrent validity of the Stryd system for measuring spatiotemporal variables during running at different velocities (8-20 km·h) by comparing data with another widely used device (the OptoGait system)...
May 17, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29781926/repeatability-and-intersession-reproducibility-of-pentacam-corneal-thickness-maps-in-fuchs-dystrophy-and-endothelial-keratoplasty
#9
David Mingo-Botín, Francisco Arnalich-Montiel, Ana Couceiro de Juan, Sara Ortiz-Toquero
PURPOSE: To assess intrasession repeatability and intersession reproducibility of Pentacam corneal thickness maps in patients with Fuchs endothelial corneal dystrophy (FECD) before and after endothelial transplantation. METHODS: In this observational diagnostic evaluation, 20 healthy subjects along with 81 consecutive patients were examined. Patients were classified into 4 groups: FECD without corneal edema, FECD with corneal edema, Descemet stripping automated endothelial keratoplasty, and Descemet membrane endothelial keratoplasty...
May 16, 2018: Cornea
https://www.readbyqxmd.com/read/29781788/a-review-of-the-reliability-of-biomechanical-variables-produced-during-the-isometric-mid-thigh-pull-and-isometric-squat-and-the-reporting-of-normative-data
#10
Claire J Brady, Andrew J Harrison, Thomas M Comyns
The use of isometric strength testing, particularly the isometric mid-thigh pull (IMTP) has increased dramatically over the last decade. The IMTP and isometric squat (ISqT) provide one aspect of performance monitoring with variables such as peak force and rate of force development being derived from the force-time curve. The reliability of some of these variables is conflicting in the literature, and the reporting of the reliability is not standardised across the research. The majority of research only reports intraclass correlation coefficients with very few studies reporting coefficient of variation and 90% confidence intervals...
May 21, 2018: Sports Biomechanics
https://www.readbyqxmd.com/read/29780979/entropy-connects-water-structure-and-dynamics-in-protein-hydration-layer
#11
Jayangika N Dahanayake, Katie R Mitchell-Koch
The enzyme Candida Antarctica lipase B (CALB) serves here as a model for understanding connections among hydration layer dynamics, solvation shell structure, and protein surface structure. The structure and dynamics of water molecules in the hydration layer were characterized for regions of the CALB surface, divided around each α-helix, β-sheet, and loop structure. Heterogeneous hydration dynamics were observed around the surface of the enzyme, in line with spectroscopic observations of other proteins. Regional differences in the structure of the biomolecular hydration layer were found to be concomitant with variations in dynamics...
May 21, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29780823/variability-in-ultrasound-backscatter-induced-by-trabecular-microstructure-deterioration-in-cancellous-bone
#12
Xingxing Chou, Feng Xu, Ying Li, Chengcheng Liu, Dean Ta, Lawrence H Le
To determine the relationship between the ultrasonic backscatter parameters and trabecular microstructural variations in cancellous bone, three erosion procedures were performed to simulate various changes in the cancellous bone microstructure. The finite difference time domain (FDTD) method was used to simulate the backscatter signal in cancellous bone. Ultrasonic backscatter properties were derived as functions of the porosity when the ultrasound incident directions were perpendicular and parallel to the major trabeculae direction (MTD), respectively...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29780626/malignant-solitary-pulmonary-nodules-assessment-of-mass-growth-rate-and-doubling-time-at-follow-up-ct
#13
Jingxu Li, Tingting Xia, Xinguan Yang, Xiao Dong, Jiamin Liang, Nanshan Zhong, Yubao Guan
Background: The differentiation of benign and malignant solitary pulmonary nodules (SPNs), especially subsolid nodules, is still challenging because of the small size, slow growth, and atypical imaging characteristics of these nodules. We aimed to determine the significance of mass growth rate (MGR) and mass doubling time (MDT) at follow-up CT of malignant SPNs. Methods: This retrospective study included 167 patients (169 SPNs, diameter 8-30 mm). Among the 169 SPNs, 114 malignant SPNs were classified into three types: pure ground-glass nodules (pGGNs), part-solid nodules (pSNs), and solid nodules (SNs)...
April 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29780236/pharmacokinetic-comparison-of-a-fixed-dose-combination-versus-concomitant-administration-of-fimasartan-amlodipine-and-rosuvastatin-using-partial-replicated-design-in-healthy-adult-subjects
#14
Minkyung Oh, Jong-Lyul Ghim, Sung-Eun Park, Eun-Young Kim, Jae-Gook Shin
Objective: The aim of this study was to compare the pharmacokinetics (PK) and safety profiles of a fixed-dose combination (FDC) formulation of fimasartan, amlodipine, and rosuvastatin with the co-administration of the two products by using a replicated crossover study design in healthy male subjects. Results: This was an open-label, randomized, three-sequence, three-period replicated crossover study in healthy male subjects. The replicated crossover design was done because of high coefficient of variation of PK parameter for fimasartan, that is, >30%...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29780047/-evaluation-of-a-2d-diode-array-corresponding-to-flattening-filter-free-x-ray-beams
#15
Tomoko Kimura, Jun-Ichi Fukunaga, Taka-Aki Hirose, Ryota Hirayama
Recently, a medical linear accelerator with a flattening filter free (FFF) mode has led to the use of FFF X-ray beams at clinical sites. The usefulness of FFF X-ray beams in high-precision radiation therapy has been reported. Therefore, the quality assurance and quality control for FFF X-ray beams have become necessary. In this study, the characteristics of the detectors of a newly developed 2-D diode array (MapCHECK2, Sun Nuclear Corporation) for FFF X-ray beams, i.e., dose reproducibility, dose rate dependence, dose linearity, and output factor, were evaluated...
2018: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/29777522/impact-of-insulin-degludec-in-hospitalized-patients-with-and-without-type-2-diabetes-requiring-parenteral-enteral-nutrition-an-observational-study
#16
Giuseppe Fatati, Agnese Di Donato, Ilenia Grandone, Pina Menicocci, Eva Mirri, Giuseppe Prosperini, Marco Scardapane, Maria Chiara Rossi, Mariangela Palazzi
INTRODUCTION: Hyperglycemia in inpatients is a major problem, especially when nutritional support is required. This study aims to assess the impact of treatment with insulin degludec (IDeg) on mean blood glucose (BG) and glycemic variability in noncritical hospitalized patients with and without type 2 diabetes (T2DM) receiving enteral and/or parenteral nutrition (EN, PN). METHODS: Mean BG and glycemic variability from admission up to 7 days of hospitalization were evaluated in consecutive cases with and without T2DM...
May 17, 2018: Advances in Therapy
https://www.readbyqxmd.com/read/29777477/cardiac-and-gait-rhythms-in-healthy-younger-and-older-adults-during-treadmill-walking-tasks
#17
Matthew W Wittstein, Joseph M Starobin, Randy J Schmitz, Sandra J Shulz, Francis Jay Haran, Christopher K Rhea
BACKGROUND: Aging and pathology result in changes in the dynamics of several physiological subsystems. Often, these changes are concurrent, altering the dynamics between subsystems. Cardiac and gait rhythms are one example in which patterns change during physical activity. AIMS: The purpose of this research is to simultaneously monitor changes in cardiac and gait rhythms when participants complete various treadmill walking tasks-normal speed, fast speed, and while synchronizing steps with a blinking metronome...
May 17, 2018: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/29777238/impact-of-body-mass-index-and-body-weight-variabilities-on-mortality-a-nationwide-cohort-study
#18
Ga Eun Nam, Kyung Hwan Cho, Kyungdo Han, Byoungduck Han, Sung Jung Cho, Yong Kyun Roh, Seon Mee Kim, Youn Seon Choi, Do Hoon Kim, Yang Hyun Kim, Yong Gyu Park
BACKGROUND/OBJECTIVES: There is limited information regarding the impact of body mass index (BMI) and body weight (BWt) variabilities on mortality. This study aimed to investigate the association between BMI, BWt variabilities and subsequent mortality in the Korean population. SUBJECTS/METHODS: This study used a representative sample cohort enrolled in the national health examination program conducted by the Korean National Health Insurance Service, and 125,391 individuals were included and followed up until 2013 (mean follow-up period = 84 months)...
May 17, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29776385/generation-of-ventilation-perfusion-ratio-map-in-surgical-patients-by-dual-energy-ct-after-xenon-inhalation-and-intravenous-contrast-media
#19
Kohei Aoki, Yotaro Izumi, Wataru Watanabe, Yuji Shimizu, Hisato Osada, Norinari Honda, Toshihide Itoh, Mitsuo Nakayama
BACKGROUND: While many studies have evaluated the change in lung volume before and after lung resection and correlated this with pulmonary function test results, there is very little evidence on the changes in ventilation perfusion ratio (V/Q) before versus after lung resection. In the present pilot study, we evaluated if V/Q mapping can be constructed using dual energy CT images. METHODS: Thirty-one lung cancer patients planned for pulmonary resection were included in this study...
May 18, 2018: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/29774894/value-of-diffusion-weighted-imaging-for-monitoring-tissue-change-during-magnetic-resonance-guided-high-intensity-focused-ultrasound-therapy-in-bone-applications-an-ex-vivo-study
#20
Sharon L Giles, Jessica M Winfield, David J Collins, Ian Rivens, John Civale, Gail R Ter Haar, Nandita M deSouza
Background: Magnetic resonance (MR)-guided high-intensity focused ultrasound (HIFU) can palliate metastatic bone pain by periosteal neurolysis. We investigated the value of diffusion-weighted imaging (DWI) for monitoring soft tissue changes adjacent to bone during MR-guided HIFU. We evaluated the repeatability of the apparent diffusion coefficient (ADC) measurement, the temporal evolution of ADC change after sonication, and its relationship with thermal parameters. Methods: Ex-vivo experiments in lamb legs ( n = 8) were performed on a Sonalleve MR-guided HIFU system...
2018: European radiology experimental
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