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J774a.1

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https://www.readbyqxmd.com/read/28527905/peroxisome-proliferator-activated-receptor-%C3%AE-mediated-polarization-of-macrophages-in-neospora-caninum-infection
#1
Xuexiu He, Pengtao Gong, Zhengkai Wei, Weijian Liu, Weili Wang, Jianhua Li, Zhengtao Yang, Xichen Zhang
BACKGROUND: Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear...
May 17, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28431321/atherogenicity-of-amino-acids-in-the-lipid-laden-macrophage-model-system-in-vitro-and-in-atherosclerotic-mice-a-key-role-for-triglyceride-metabolism
#2
Oren Rom, Claudia Grajeda-Iglesias, Mahmoud Najjar, Niroz Abu-Saleh, Nina Volkova, Dalit Esther Dar, Tony Hayek, Michael Aviram
Atherosclerosis-related research has focused mainly on the effects of lipids on macrophage foam cell formation and atherogenesis, whereas the role of amino acids (AAs) was understudied. The current study aimed to identify anti- or pro-atherogenic AA in the macrophage model system and to elucidate the underlying metabolic and molecular mechanisms. J774A.1 cultured macrophages were treated with increasing concentrations of each 1 of the 20 AAs. Macrophage atherogenicity was assessed in terms of cellular toxicity, generation of reactive oxygen species (ROS) and cellular cholesterol or triglyceride content...
April 6, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28420125/bioavailable-citrus-sinensis-extract-polyphenolic-composition-and-biological-activity
#3
Giacomo Pepe, Francesco Pagano, Simona Adesso, Eduardo Sommella, Carmine Ostacolo, Michele Manfra, Marcello Chieppa, Marina Sala, Mariateresa Russo, Stefania Marzocco, Pietro Campiglia
Citrus plants contain large amounts of flavonoids with beneficial effects on human health. In the present study, the antioxidant and anti-inflammatory potential of bioavailable polyphenols from Citrus sinensis was evaluated in vitro and ex vivo, using the murine macrophages cell line J774A.1 and primary peritoneal macrophages. Following simulated gastro-intestinal digestion, the in vitro bioavailability of Citrus sinensis polyphenolic extract was assessed using the human cell line Caco-2 grown as monolayers on a transwell membrane...
April 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28419526/soluble-egg-antigen-activates-m2-macrophages-via-the-stat6-and-pi3k-pathways-and-schistosoma-japonicum-alternatively-activates-macrophage-polarization-to-improve-the-survival-rate-of-septic-mice
#4
Hao Tang, Yan-Bing Liang, Zhi-Bin Chen, Lin-Lin Du, Li-Jin Zeng, Jing-Guo Wu, Wen Yang, Hua-Ping Liang, Zhong-Fu Ma
Sepsis is one of the most challenging health problems worldwide. Our previous study showed that chronic schistosoma japonica (SJ) infection might increase serum anti-inflammatory factors to play a protective role, thus improving the survival rate of septic mice. Further research revealed that SJ infection promoted J774A.1 macrophage differentiation into M2 macrophages; suppressed LPS-induced activation of M1 macrophages; up-regulated CD163, IL-10, and TGF-β1 expression; inhibited TNF-α and iNOS expression; and blocked the effect of LPS-promoted TNF-α and iNOS expression...
April 17, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28398240/anti-inflammatory-antioxidant-and-crystallographic-studies-of-n-palmitoyl-ethanol-amine-pea-derivatives
#5
Carmela Saturnino, Ada Popolo, Anna Ramunno, Simona Adesso, Michela Pecoraro, Maria Rosaria Plutino, Silvia Rizzato, Alberto Albinati, Stefania Marzocco, Marina Sala, Domenico Iacopetta, Maria Stefania Sinicropi
N-Palmitoyl-ethanolamine (PEA) is an anti-inflammatory component of egg yolk that is usually employed for the prevention of respiratory apparatus virus infection and then frequently used for its efficient anti-inflammatory and analgesic effects in experimental models of visceral, neuropathic, and inflammatory diseases. Nevertheless, data of its use in animal or human therapy are still scarce and further studies are needed. Herein, we report the biological evaluation of a small library of N-palmitoyl-ethanolamine analogues or derivatives, characterized by a protected acid function (either as palmitoyl amides or hexadecyl esters), useful to decrease their hydrolysis rate in vitro and prolong their biological activity...
April 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28394618/potential-eye-drop-based-on-a-calix-4-arene-nanoassembly-for-curcumin-delivery-enhanced-drug-solubility-stability-and-anti-inflammatory-effect
#6
Giuseppe Granata, Irene Paterniti, Corrada Geraci, Francesca Cunsolo, Emanuela Esposito, Marika Cordaro, Anna Rita Blanco, Salvatore Cuzzocrea, Grazia M L Consoli
Curcumin is an Indian spice with a wide spectrum of biological and pharmacological activities but poor aqueous solubility, rapid degradation, and low bioavailability that affect medical benefits. To overcome these limits in ophthalmic application, curcumin was entrapped in a polycationic calix[4]arene-based nanoaggregate by a simple and reproducible method. The calix[4]arene-curcumin supramolecular assembly (Calix-Cur) appeared as a clear colloidal solution consisting in micellar nanoaggregates with size, polydispersity index, surface potential, and drug loading percentage meeting the requirements for an ocular drug delivery system...
April 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28368124/development-of-surface-variable-polymeric-nanoparticles-for-drug-delivery-to-tumors
#7
Ning Han, Liang Pang, Jun Xu, Hyesun Hyun, Jinho Park, Yoon Yeo
To develop nanoparticle drug carriers that interact with cells specifically in the mildly acidic tumor microenvironment, we produced polymeric nanoparticles modified with amidated TAT peptide via a simple surface modification method. Two types of core poly(lactic-co-glycolic acid) nanoparticles (NL and NP) were prepared with a phospholipid shell as an optional feature and covered with polydopamine that enabled the conjugation of TAT peptide on the surface. Subsequent treatment with acid anhydrides such as cis-aconitic anhydride (CA) and succinic anhydride (SA) converted amines of lysine residues in TAT peptide to β-carboxylic amides, introducing carboxylic groups that undergo pH-dependent protonation and deprotonation...
April 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28365379/phosphotransferase-systems-in-enterococcus-faecalis-og1rf-enhance-anti-stress-capacity-in%C3%A2-vitro-and-in%C3%A2-vivo
#8
Zhen Peng, Matthias A Ehrmann, Anna Waldhuber, Christine Niemeyer, Thomas Miethke, Julia-Stefanie Frick, Tao Xiong, Rudi F Vogel
Phosphotransferase systems are common and essential in bacteria, which are in charge of sugar transportation and phosphorylation. However, phosphotransferase systems were found in recent years to be associated with environmental stress factors. This study investigated the role of the mannose/fructose/sorbose phosphotransferase systems in Enterococcus faecalis OG1RF in adaption to harsh environments by construction of pts mutants. More than one mannose/fructose/sorbose phosphotransferase system was found in E...
March 29, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28361953/cell-to-cell-transfer-of-saa1-protein-in-a-cell-culture-model-of-systemic-aa-amyloidosis
#9
Stephanie Claus, Ioana Puscalau-Girtu, Paul Walther, Tatiana Syrovets, Thomas Simmet, Christian Haupt, Marcus Fändrich
Systemic AA amyloidosis arises from the misfolding of serum amyloid A1 (SAA1) protein and the deposition of AA amyloid fibrils at multiple sites within the body. Previous research already established that mononuclear phagocytes are crucial for the formation of the deposits in vivo and exposure of cultures of such cells to SAA1 protein induces the formation of amyloid deposits within the culture dish. In this study we show that both non-fibrillar and fibrillar SAA1 protein can be readily transferred between cultured J774A...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300196/recombinant-mycobacterium-smegmatis-with-a-pmyong2-vector-expressing-human-immunodeficiency-virus-type-i-gag-can-induce-enhanced-virus-specific-immune-responses
#10
Byoung-Jun Kim, Jeong-Ryeol Gong, Ga-Na Kim, Bo-Ram Kim, So-Young Lee, Yoon-Hoh Kook, Bum-Joon Kim
Recently, we have developed a novel Mycobacterium-Escherichia coli shuttle vector system using pMyong2, which can provide an enhanced expression of heterologous genes in recombinant Mycobacterium smegmatis (rSmeg). To investigate the usefulness of rSmeg using pMyong2 in vaccine application, we vaccinated M. smegmatis with pMyong2 system expressing Human Immunodeficiency Virus Type I (HIV-1) Gag p24 antigen (rSmeg-pMyong2-p24) into mice and examined its cellular and humoral immune responses against HIV gag protein...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28275134/the-complement-anaphylatoxins-c5a-and-c3a-suppress-ifn-%C3%AE-production-in-response-to-listeria-monocytogenes-by-inhibition-of-the-cyclic-dinucleotide-activated-cytosolic-surveillance-pathway
#11
Stacey L Mueller-Ortiz, Daniel G Calame, Nancy Shenoi, Yi-Dong Li, Rick A Wetsel
Listeria monocytogenes is an intracellular Gram-positive bacterium that induces expression of type I IFNs (IFN-α/IFN-β) during infection. These cytokines are detrimental to the host during infection by priming leukocytes to undergo L. monocytogenes-mediated apoptosis. Our previous studies showed that C5aR1(-/-) and C3aR(-/-) mice are highly susceptible to L. monocytogenes infection as a result of increased IFN-β-mediated apoptosis of major leukocyte cell populations, including CD4(+) and CD8(+) T cells. However, the mechanisms by which C3a and C5a modulate IFN-β expression during L...
April 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28268194/a-bioluminescent-caspase-1-activity-assay-rapidly-monitors-inflammasome-activation-in-cells
#12
Martha O'Brien, Danielle Moehring, Raúl Muñoz-Planillo, Gabriel Núñez, Justin Callaway, Jenny Ting, Mike Scurria, Tim Ugo, Laurent Bernad, James Cali, Dan Lazar
Inflammasomes are protein complexes induced by diverse inflammatory stimuli that activate caspase-1, resulting in the processing and release of cytokines, IL-1β and IL-18, and pyroptosis, an immunogenic form of cell death. To provide a homogeneous method for detecting caspase-1 activity, we developed a bioluminescent, plate-based assay that combines a substrate, Z-WEHD-aminoluciferin, with a thermostable luciferase in an optimized lytic reagent added directly to cultured cells. Assay specificity for caspase-1 is conferred by inclusion of a proteasome inhibitor in the lytic reagent and by use of a caspase-1 inhibitor to confirm activity...
March 6, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28237876/passive-transfer-of-interferon-%C3%AE-over-expressing-macrophages-enhances-resistance-of-scid-mice-to-mycobacterium-tuberculosis-infection
#13
Rajamouli Pasula, William J Martin, Banu Rekha Kesavalu, Maher Y Abdalla, Bradley E Britigan
Infection with Mycobacterium tuberculosis (M.tb) is associated with increased deaths worldwide. Alveolar macrophages (AMs) play a critical role in host defense against infection with this pathogen. In this work we tested the hypothesis that passive transfer of normal AMs, IFN-γ activated AMs, or macrophages transduced to over-express IFN-γ into the lungs of immunosuppressed SCID mice, where resident macrophages are present but not functional, would enhance alveolar immunity and increase clearance of pulmonary M...
February 23, 2017: Cytokine
https://www.readbyqxmd.com/read/28208020/graphene-oxide-induced-perturbation-to-plasma-membrane-and-cytoskeletal-meshwork-sensitize-cancer-cells-to-chemotherapeutic-agents
#14
Jianqiang Zhu, Ming Xu, Ming Gao, Zhihong Zhang, Yong Xu, Tian Xia, Sijin Liu
The outstanding physicochemical properties endow graphene materials (e.g., graphene oxide, GO) with beneficial potentials in diverse biomedical fields such as bioimaging, drug delivery, and biomolecular detection. GO recently emerged as a chemosensitizer; however, the detailed molecular basis underlying GO-conducted sensitization and corresponding biological effects are still elusive. Based on our recent findings that GO treatment at sublethal concentrations could impair the general cellular priming state, including disorders of plasma membrane and cytoskeleton construction, we aimed here to explore the mechanism of GO as a sensitizer to make cancer cells more susceptible to chemotherapeutic agents...
March 28, 2017: ACS Nano
https://www.readbyqxmd.com/read/28167209/efficacy-of-protease-inhibitor-from-marine-streptomyces-sp-vitbvk2-against-leishmania-donovani-an-in-vitro-study
#15
Veena Sreedharan, K V Bhaskara Rao
In the present study the leishmanicidal effect of potential protease inhibitor producing marine actinobacterial isolate has been investigated against Leishmania donovani, the causative agent of visceral leishmaniasis. Among 89 marine actinobacteria isolated from a salt pan in Kanyakumari, only one isolate (BVK2) showed 97% of protease inhibition activity against trypsin. Moderate to high protease inhibitor activity was shown by isolate BVK2 on proteinase (30%) and chymotrypsin (85%). In optimization study for protease inhibitor production glucose as carbon source and casein as nitrogen source showed the best activity...
March 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28142331/differential-cytotoxic-and-inflammatory-potency-of-amorphous-silicon-dioxide-nanoparticles-of-similar-size-in-multiple-cell-lines
#16
Dalibor Breznan, Dharani D Das, Julie S O'Brien, Christine MacKinnon-Roy, Surendra Nimesh, Ngoc Q Vuong, Stéphane Bernatchez, Nimal DeSilva, Myriam Hill, Prem Kumarathasan, Renaud Vincent
The likelihood of environmental and health impacts of silicon dioxide nanoparticles (SiNPs) has risen, due to their increased use in products and applications. The biological potency of a set of similarly-sized amorphous SiNPs was investigated in a variety of cells to examine the influence of physico-chemical and biological factors on their toxicity. Cellular LDH and ATP, BrdU incorporation, resazurin reduction and cytokine release were measured in human epithelial A549, human THP-1 and mouse J774A.1 macrophage cells exposed for 24 h to suspensions of 5-15, 10-20 and 12 nm SiNPs and reference particles...
March 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28122883/retinol-binding-protein-dependent-cholesterol-uptake-regulates-macrophage-foam-cell-formation-and-promotes-atherosclerosis
#17
Yan Liu, Yuan Zhong, Hongen Chen, Duan Wang, Min Wang, Jing-Song Ou, Min Xia
BACKGROUND: Retinol-binding protein 4 (RBP4) is an adipokine that plays decisive roles in glucose metabolism and insulin sensitivity. Elevated circulating RBP4 levels were reported to be associated with increased risk for cardiovascular disease, but the precise role of RBP4 in atherosclerotic diseases and its mechanisms of action remain elusive. METHODS: Serum RBP4 levels of 1683 participants from South China were evaluated and the occurrence of major adverse cardiovascular events was followed up for 5 years...
April 4, 2017: Circulation
https://www.readbyqxmd.com/read/28062849/interplay-between-a-cytosolic-and-a-cell-surface-carbonic-anhydrase-in-ph-homeostasis-and-acid-tolerance-of-leishmania
#18
Dhiman Sankar Pal, Mazharul Abbasi, Dipon Kumar Mondal, Binitha Anu Varghese, Ritama Paul, Shalini Singh, Rupak Datta
Leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. How Leishmania cells maintain near-neutral intracellular pH and proliferate in such proton-rich milieu remains poorly understood. We report here that for thriving in acidic condition Leishmania major relies on a cytosolic and a cell surface carbonic anhydrase viz. LmCA1 and LmCA2. Upon exposure to acidic medium, intracellular pH of the LmCA1(+/-), LmCA2(+/-) and LmCA1(+/-): LmCA2(+/-) mutant strains dropped by varying extents that led to cell cycle delay, growth retardation and morphological abnormalities...
January 6, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28049022/albumin-coated-nanocrystals-for-carrier-free-delivery-of-paclitaxel
#19
Joonyoung Park, Bo Sun, Yoon Yeo
Nanoparticles are used to deliver anticancer drugs to solid tumors. However, clinical development of nanoparticles is challenging because of their limitations in physicochemical properties, such as low drug loading efficiency and poor circulation stability. Low drug loading not only causes technical difficulty in administration but also increases the amount of co-delivered carrier materials, imposing biological burdens to patients. Poor circulation stability causes loss of pharmacokinetics benefits of nanoparticles...
December 31, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28002746/frustrated-phagocytic-spreading-of-j774a-1-macrophages-ends-in-myosin-ii-dependent-contraction
#20
Daniel T Kovari, Wenbin Wei, Patrick Chang, Jan-Simon Toro, Ruth Fogg Beach, Dwight Chambers, Karen Porter, Doyeon Koo, Jennifer E Curtis
Conventional studies of dynamic phagocytic behavior have been limited in terms of spatial and temporal resolution due to the inherent three-dimensionality and small features of phagocytosis. To overcome these issues, we use a series of frustrated phagocytosis assays to quantitatively characterize phagocytic spreading dynamics. Our investigation reveals that frustrated phagocytic spreading occurs in phases and is punctuated by a distinct period of contraction. The spreading duration and peak contact areas are independent of the surface opsonin density, although the opsonin density does affect the likelihood that a cell will spread...
December 20, 2016: Biophysical Journal
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