keyword
MENU ▼
Read by QxMD icon Read
search

J774a.1

keyword
https://www.readbyqxmd.com/read/28928657/colocynth-extracts-prevent-epithelial-to-mesenchymal-transition-and-stemness-of-breast-cancer-cells
#1
Kaushik Chowdhury, Ankit Sharma, Suresh Kumar, Gyanesh K Gunjan, Alo Nag, Chandi C Mandal
Modern treatment strategies provide better overall survival in cancer patients, primarily by controlling tumor growth. However, off-target and systemic toxicity, tumor recurrence, and resistance to therapy are still inadvertent hurdles in current treatment regimens. Similarly, metastasis is another deadly threat to patients suffering from cancer. This has created an urgent demand to come up with new drugs having anti-metastatic potential and minimum side effects. Thus, this study was aimed at exploring the anti-proliferative and anti-metastatic potential of colocynth medicinal plant...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28822264/trimethylamine-n-oxide-as-a-risk-factor-for-atherosclerosis-induces-stress-in-j774a-1-murine-macrophages
#2
Abbas Mohammadi, Zakaria Vahabzadeh, Soran Jamalzadeh, Tahereh Khalili
PURPOSE: Trimethylamine N-oxide (TMAO) is a biomarker for kidney problems. It has also been introduced as a risk factor for atherosclerosis. The classic risk factors for atherosclerosis trigger cellular and humeral immunoreaction in macrophages through induction of heat shock protein expressions and increased levels of GRP94 and HSP70 are associated with increased atherosclerosis risk. The present study evaluated the possible effect(s) of TMAO on the expression of GRP94 and HSP70 at protein levels...
August 16, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28791964/effect-of-copper-on-the-up-regulation-down-regulation-of-genes-cytotoxicity-and-ion-dissolution-for-mesoporous-bioactive-glasses
#3
Gurbinder Kaur, N Sriranganathan, S G Waldrop, Purnima Sharma, B N Chudasama
In the present study, copper-based (25 - x)CaO - xCuO -10P2O5 - 5B2O3 - 60SiO2 (x = 2.5, 5, 7.5, 10 mol%) mesoporous bioactive glasses (MBGs) were synthesized using the sol-gel technique with cetyltrimethyl ammonium bromide as the structure-directing agent. The live-dead cell count and cytocompatibility of MBGs for J774A.1 murine macrophages have also been investigated for different concentrations of MBGs. The ionic dissolution profile for Ca, P and Si has been evaluated in the simulated body fluid. The effect of copper content as well as the ionic dissolution products on the up-regulation and down-regulation of TGIF-2, HDAC-4, Smurf-1, mir-30c and mir-130a genes for the murine model are investigated using reverse transcription-polymerase chain reaction...
August 9, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28791016/doxycycline-attenuates-leptospira-induced-il-1%C3%AE-by-suppressing-nlrp3-inflammasome-priming
#4
Wenlong Zhang, Xufeng Xie, Dianjun Wu, Xuemin Jin, Runxia Liu, Xiaoyu Hu, Yunhe Fu, Zhuang Ding, Naisheng Zhang, Yongguo Cao
Doxycycline (Dox), a semisynthetic antibiotic, has been reported to exert multiple immunomodulatory effects. Treatment with Dox has a satisfactory curative effect against leptospirosis. In addition to its antibacterial action, we supposed that Dox also modulated immune response in controlling leptospira infection. Using J774A.1 mouse macrophages, the effects of Dox on protein and mRNA levels of IL-1β and TNF-α were investigated after infection with live or sonicated Leptospira interrogans serovar Lai strain Lai (56601)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28770171/ul36-rescues-apoptosis-inhibition-and-in-vivo-replication-of-a-chimeric-mcmv-lacking-the-m36-gene
#5
M Zeeshan Chaudhry, Bahram Kasmapour, Carlos Plaza-Sirvent, Milica Bajagic, Rosaely Casalegno Garduño, Lisa Borkner, Tihana Lenac Roviš, Andrea Scrima, Stipan Jonjic, Ingo Schmitz, Luka Cicin-Sain
Apoptosis is an important defense mechanism mounted by the immune system to control virus replication. Hence, cytomegaloviruses (CMV) evolved and acquired numerous anti-apoptotic genes. The product of the human CMV (HCMV) UL36 gene, pUL36 (also known as vICA), binds to pro-caspase-8, thus inhibiting death-receptor apoptosis and enabling viral replication in differentiated THP-1 cells. In vivo studies of the function of HCMV genes are severely limited due to the strict host specificity of cytomegaloviruses, but CMV orthologues that co-evolved with other species allow the experimental study of CMV biology in vivo...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28766178/differential-regulation-of-il-1%C3%AE-and-il-6-release-in-murine-macrophages
#6
Seishiro Hirano, Quan Zhou, Akiko Furuyama, Sanae Kanno
Asbestos and silica (exogenous danger) and adenosine triphosphate (ATP, endogenous danger-signaling molecule) synergistically increase IL-1β release from endotoxin-primed macrophage, which is mediated by NOD-like receptor protein 3 (NLRP3) inflammasome. However, the conversion of pro-IL-1β to its active form seems to depend on the macrophage cell types. In the present study, bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1β and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1β from pro-IL-1β...
August 1, 2017: Inflammation
https://www.readbyqxmd.com/read/28737923/cosilencing-intestinal-transglutaminase-2-and-interleukin-15-using-gelatin-based-nanoparticles-in-an-in-vitro-model-of-celiac-disease
#7
Husain Attarwala, Valerie Clausen, Prasoon Chaturvedi, Mansoor M Amiji
In this study, we have developed a type B gelatin nanoparticle based siRNA delivery system for silencing of intestinal transglutaminase-2 (TG2) and interleukin-15 (IL-15) genes in cultured human intestinal epithelial cells (Caco-2) and murine alveolar macrophage cells (J774A.1). Small interfering RNA (siRNA) targeting the TG2 or IL-15 gene was encapsulated within gelatin nanoparticles using ethanol-water solvent displacement method. Size, charge, and morphology of gelatin nanoparticles were evaluated using a Zetasizer instrument and transmission electron microscopy...
August 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28716750/hexadecylphosphocholine-miltefosine-stabilized-chitosan-modified-ampholipospheres-as-prototype-co-delivery-vehicle-for-enhanced-killing-of-l-donovani
#8
Priyanka Tripathi, Anil Kumar Jaiswal, Anuradha Dube, Prabhat Ranjan Mishra
Lipid nanoparticles are stable, biodegradable and biocompatible carriers offering excellent therapeutic efficacy. Here, a novel effort has been made to develop Miltefosine (HePC- hexadecylphosphocholine) stabilized chitosan anchored nanostructured lipid carriers (NLC) of Amphotericin B (AmB) as co-delivery vehicle to enhance killing of L. donovani. The entrapment efficiency of AmB was achieved upto 85.3% for HePC-AmB-CNLCs with mean particle size of 150.8±8.4nm, and zeta potential value of +28.2±1.1mV, respectively...
July 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28669709/a-mechanistic-insight-into-curcumin-modulation-of-the-il-1%C3%AE-secretion-and-nlrp3-s-glutathionylation-induced-by-needle-like-cationic-cellulose-nanocrystals-in-myeloid-cells
#9
Andrew Guglielmo, Adham Sabra, Mostafa Elbery, Milena M Cerveira, Fernanda Ghenov, Rajesh Sunasee, Karina Ckless
Recently we have demonstrated that needle-like cationic cellulose nanocrystals (CNC-AEMA2) evoke immunological responses through NLRP3 inflammasome/IL-1β inflammatory pathway. In this study we demonstrated that curcumin, a naturally occurring polyphenolic compound isolated from Curcuma longa (Zingiberaceae), was able to suppress, at least in part, this immunological response, as observed by diminished IL-1β secretion in CNC-AEMA2-stimulated macrophages primed with LPS. Curcumin is a well-known antioxidant and anti-inflammatory natural compound and in addition to acting as "scavenger" of reactive oxygen species (ROS), it can also upregulates antioxidant enzymes...
August 25, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28666415/soluble-factors-from-biofilm-of-candida-albicans-and-staphylococcus-aureus-promote-cell-death-and-inflammatory-response
#10
Kassia de Carvalho Dias, Paula Aboud Barbugli, Fernanda de Patto, Virginia Barreto Lordello, Letícia de Aquino Penteado, Alexandra Ivo Medeiros, Carlos Eduardo Vergani
BACKGROUND: The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species Candida albicans (C. albicans) and methicillin-sensitive Staphylococcus aureus (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of C. albicans and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT)...
June 30, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28653829/development-and-characterization-of-a-hydroxyl-sulfonamide-analogue-5-chloro-n-2-4-hydroxysulfamoyl-phenyl-ethyl-2-methoxy-benzamide-as-a-novel-nlrp3-inflammasome-inhibitor-for-potential-treatment-of-multiple-sclerosis
#11
Chunqing Guo, Jacob W Fulp, Yuqi Jiang, Xia Li, Jeremy E Chojnacki, Jingde Wu, Xiang-Yang Wang, Shijun Zhang
In our efforts to develop novel small-molecule inhibitors for the NOD-like receptor family pyrin-domain-containing 3 (NLRP3) inflammasome as potential disease-modifying agents to treat neurological disorders including multiple sclerosis (MS), a hydroxyl sulfonamide analogue JC-171 has been rationally designed and biologically characterized both in vitro and in vivo. Our studies established that JC-171 dose dependently inhibited LPS/ATP-induced interleukin-1β (IL-1β) release from J774A.1 macrophages with an IC50 of 8...
July 13, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28651168/the-antioxidant-and-anti-inflammatory-properties-of-lycopene-in-mice-lungs-exposed-to-cigarette-smoke
#12
Keila Karine Duarte Campos, Glaucy Rodrigues Araújo, Thais Lourenço Martins, Ana Carla Balthar Bandeira, Guilherme de Paula Costa, André Talvani, Camila Carrião Machado Garcia, Laser Antônio Machado Oliveira, Daniela Caldeira Costa, Frank Silva Bezerra
Lycopene is a carotenoid with known antioxidant and anti-inflammatory properties. We aimed to evaluate the in vitro and in vivo effects of lycopene on reducing the redox imbalance and inflammation induced by cigarette smoke (CS). For the in vitro study, J774A.1 (macrophages) cells were incubated in the presence of 0.5, 1.0, 2.0, 4.0, 8.0, 10.0 and 25 μM of lycopene for 3, 6 and 24 h or in the presence of 0.1%, 0.25%, 0.5%, 0.625%, 1.25%, 2.25%, 5% and 10% cigarette smoke extract (CSE) for 3, 6 and 24 h to assess cell viability and measurement of intracellular reactive oxygen species (ROS)...
October 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#13
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonasaeruginosa causes lung infections in patients with cystic fibrosis (CF). The Pseudomonas quinolone signal (PQS) compound is a secreted P. aeruginosa virulence factor that contributes to the pathogenicity of P. aeruginosa We were able to detect PQS in sputum samples from CF patients infected with P. aeruginosa but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce the production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial [NHBE]) cells and macrophages (J774A...
September 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28621735/anti-inflammatory-and-anti-oxidant-potential-of-the-root-extract-and-constituents-of-doronicum-austriacum
#14
Stefania Marzocco, Simona Adesso, Mostafa Alilou, Hermann Stuppner, Stefan Schwaiger
Doronicum austriacum Jacq., Asteraceae, is a plant which is used in traditional alpine medicine. Historical sources describe the medical use of the root, but up until now only a few studies evaluated its pharmacological properties. The evaluation of the dichloromethane extract, and its major compounds for their anti-inflammatory and anti-oxidant potential was performed in macrophages J774A.1 and C6 astrocytes. Nitric oxide (NO) and reactive oxygen species (ROS) release, as well as nitrotyrosine formation, were evaluated...
June 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28608692/galactomannan-from-antrodia-cinnamomea-enhances-the-phagocytic-activity-of-macrophages
#15
Namal Perera, Feng-Ling Yang, Ching-Ming Chang, Yueh-Tung Lu, Shih-Hao Zhan, Yi-Ting Tsai, Jung-Feng Hsieh, Lan-Hui Li, Kuo-Feng Hua, Shih-Hsiung Wu
Galactomannan with an octasaccharide-repeating unit (ACP) was isolated from Taiwan medicinal mushroom Antrodia cinnamomea, and its chemical structure was determined herein. ACP significantly enhanced the phagocytosis and bactericidal activity of J774A.1 murine macrophages against Escherichia coli, with prospects for developing a new immunomodulatory compound or adjuvant in immunotherapy and vaccination.
June 13, 2017: Organic Letters
https://www.readbyqxmd.com/read/28605335/in-vitro-expression-of-toll-like-receptors-and-proinflammatory-molecules-induced-by-ergosta-7-22-dien-3-one-isolated-from-a-wild-mexican-strain-of-ganoderma-oerstedii-agaricomycetes
#16
Angel Ramos-Ligonio, Aracely López-Monteon, Maria de la Soledad Lagunes-Castro, Jorge Suarez-Medellin, Cesar Espinoza, Guillermo Mendoza, Angel Trigos
Compounds showing pharmacological activity on the immune system are of interest because of their therapeutic potential in the treatment of many diseases. However, data from primary human immune cells and in vivo studies are limited. The aim of this study was to analyze the ability to induce the expression of Toll-like receptors (TLRs) and proinflammatory molecules on cells involved in the immune system using the compound ergosta-7,22-dien-3- one, isolated from a wild Mexican strain of Ganoderma oerstedii. According to our study, ergosta-7,22-dien-3-one did not present any cytotoxic effect on HeLa or J774A...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28600163/involvement-of-tyrosine-specific-protein-kinase-and-protein-kinase-c-in-j774a-1-macrophage-functions-activated-by-tinospora-cordifolia
#17
Priti More, Kalpana Pai
BACKGROUND: Macrophages are the first line of defense and constitute important participant in the bi-directional interaction between innate and specific immunity. Macrophages are in a quiescent form and get activated when given a stimulus. In our previous studies we have reported that guduchi or LPS treatment of macrophages enhanced production of nitric oxide (NO) and increased tumoricidal activity against L929 fibroblast cells. OBJECTIVE: In the present study effect of Tinospora cordifolia commonly known as guduchi on macrophage activation and the mechanism of action i...
April 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/28582854/activation-of-macrophages-and-microglia-by-interferon-%C3%AE-and-lipopolysaccharide-increases-methylglyoxal-production-a-new-mechanism-in-the-development-of-vascular-complications-and-cognitive-decline-in-type-2-diabetes-mellitus
#18
Karthik Dhananjayan, Dhanushka Gunawardena, Nerissa Hearn, Tanja Sonntag, Chris Moran, Erika Gyengesi, Velandai Srikanth, Gerald Münch
Methylglyoxal (MGO), a dicarbonyl compound derived from glucose, is elevated in diabetes mellitus and contributes to vascular complications by crosslinking collagen and increasing arterial stiffness. It is known that MGO contributes to inflammation as it forms advanced glycation end products (AGEs), which activate macrophages via the receptor RAGE. The aim of study was to investigate whether inflammatory activation can increase MGO levels, thereby completing a vicious cycle. In order to validate this, macrophage (RAW264...
May 30, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28575082/the-role-of-reactive-oxygen-intermediates-in-the-intracellular-fate-of-leptospira-interrogans-in-the-macrophages-of-different-hosts
#19
Shijun Li, Peili Li, Lei Zhang, Weilin Hu, Ming Wang, Ying Liu, Guangpeng Tang, Dingming Wang, Bijun Zhou, Jie Yan
BACKGROUND: Pathogenic species of Leptospira cause leptospirosis, a global zoonotic disease. Our previous work showed that leptospires survive and replicate in human macrophages but are killed in murine macrophages. However, the mechanism responsible for the different intracellular fates of leptospires within the macrophages of different hosts remains unclear. RESULTS: The present study demonstrates that infection with Leptospira interrogans caused significant up-regulation of reactive oxygen species (ROS) and superoxide in J774A...
2017: PloS One
https://www.readbyqxmd.com/read/28527905/peroxisome-proliferator-activated-receptor-%C3%AE-mediated-polarization-of-macrophages-in-neospora-caninum-infection
#20
Xuexiu He, Pengtao Gong, Zhengkai Wei, Weijian Liu, Weili Wang, Jianhua Li, Zhengtao Yang, Xichen Zhang
BACKGROUND: Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear...
July 2017: Experimental Parasitology
keyword
keyword
35409
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"