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https://www.readbyqxmd.com/read/28208020/graphene-oxide-induced-perturbation-to-plasma-membrane-and-cytoskeletal-meshwork-sensitize-cancer-cells-to-chemotherapeutic-agents
#1
Jianqiang Zhu, Ming Xu, Ming Gao, Zhihong Zhang, Yong Xu, Tian Xia, Sijin Liu
The outstanding physicochemical properties endow graphene materials (e.g. graphene oxide, GO) with beneficial potentials in diverse biomedical fields such as bioimaging, drug delivery and biomolecular detection. GO recently emerges to act as chemosensitizer; however, the detailed molecular bases underlying GO-conducted sensitization and according biological effects are still elusive. Based on our recent findings that GO treatment at sublethal concentrations could impair the general cellular priming state including disorders of plasma membrane and cytoskeleton construction, we here aimed to explore the mechanism of GO as a sensitizer to make cancer cells more susceptible to chemotherapeutic agents...
February 16, 2017: ACS Nano
https://www.readbyqxmd.com/read/28167209/efficacy-of-protease-inhibitor-from-marine-streptomyces-sp-vitbvk2-against-leishmania-donovani-an-in-vitro-study
#2
Veena Sreedharan, K V Bhaskara Rao
In the present study the leishmanicidal effect of potential protease inhibitor producing marine actinobacterial isolate has been investigated against Leishmania donovani, the causative agent of visceral leishmaniasis. Among 89 marine actinobacteria isolated from a salt pan in Kanyakumari, only one isolate (BVK2) showed 97% of protease inhibition activity against trypsin. Moderate to high protease inhibitor activity was shown by isolate BVK2 on proteinase (30%) and chymotrypsin (85%). In optimization study for protease inhibitor production glucose as carbon source and casein as nitrogen source showed the best activity...
February 4, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28142331/differential-cytotoxic-and-inflammatory-potency-of-amorphous-silicon-dioxide-nanoparticles-of-similar-size-in-multiple-cell-lines
#3
Dalibor Breznan, Dharani D Das, Julie S O'Brien, Christine MacKinnon-Roy, Surendra Nimesh, Ngoc Q Vuong, Stéphane Bernatchez, Nimal DeSilva, Myriam Hill, Prem Kumarathasan, Renaud Vincent
The likelihood of environmental and health impacts of silicon dioxide nanoparticles (SiNPs) has risen, due to their increased use in products and applications. The biological potency of a set of similarly-sized amorphous SiNPs was investigated in a variety of cells to examine the influence of physico-chemical and biological factors on their toxicity. Cellular LDH and ATP, BrdU incorporation, resazurin reduction and cytokine release were measured in human epithelial A549, human THP-1 and mouse J774A.1 macrophage cells exposed for 24 h to suspensions of 5-15, 10-20 and 12 nm SiNPs and reference particles...
February 1, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28122883/retinol-binding-protein-dependent-cholesterol-uptake-regulates-macrophage-foam-cell-formation-and-promotes-atherosclerosis
#4
Yan Liu, Yuan Zhong, Hongen Chen, Duan Wang, Min Wang, Jing-Song Ou, Min Xia
BACKGROUND: -Retinol binding protein 4 (RBP4) is an adipokine that plays decisive roles in glucose metabolism and insulin sensitivity. Elevated circulating RBP4 levels were reported to be associated with increased risk for cardiovascular disease, but the precise role of RBP4 in atherosclerotic diseases and its mechanisms of action remain elusive. METHODS: -Serum RBP4 levels of 1683 participants from South China were evaluated and the occurrence of major adverse cardiovascular events was followed up for 5 years...
January 25, 2017: Circulation
https://www.readbyqxmd.com/read/28062849/interplay-between-a-cytosolic-and-a-cell-surface-carbonic-anhydrase-in-ph-homeostasis-and-acid-tolerance-of-leishmania
#5
Dhiman Sankar Pal, Mazharul Abbasi, Dipon Kumar Mondal, Binitha Anu Varghese, Ritama Paul, Shalini Singh, Rupak Datta
Leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. How Leishmania cells maintain near-neutral intracellular pH and proliferate in such proton-rich milieu remains poorly understood. We report here that for thriving in acidic condition Leishmania major relies on a cytosolic and a cell surface carbonic anhydrase viz. LmCA1 and LmCA2. Upon exposure to acidic medium, intracellular pH of the LmCA1(+/-), LmCA2(+/-) and LmCA1(+/-): LmCA2(+/-) mutant strains dropped by varying extents that led to cell cycle delay, growth retardation and morphological abnormalities...
January 6, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28049022/albumin-coated-nanocrystals-for-carrier-free-delivery-of-paclitaxel
#6
Joonyoung Park, Bo Sun, Yoon Yeo
Nanoparticles are used to deliver anticancer drugs to solid tumors. However, clinical development of nanoparticles is challenging because of their limitations in physicochemical properties, such as low drug loading efficiency and poor circulation stability. Low drug loading not only causes technical difficulty in administration but also increases the amount of co-delivered carrier materials, imposing biological burdens to patients. Poor circulation stability causes loss of pharmacokinetics benefits of nanoparticles...
December 31, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28002746/frustrated-phagocytic-spreading-of-j774a-1-macrophages-ends-in-myosin-ii-dependent-contraction
#7
Daniel T Kovari, Wenbin Wei, Patrick Chang, Jan-Simon Toro, Ruth Fogg Beach, Dwight Chambers, Karen Porter, Doyeon Koo, Jennifer E Curtis
Conventional studies of dynamic phagocytic behavior have been limited in terms of spatial and temporal resolution due to the inherent three-dimensionality and small features of phagocytosis. To overcome these issues, we use a series of frustrated phagocytosis assays to quantitatively characterize phagocytic spreading dynamics. Our investigation reveals that frustrated phagocytic spreading occurs in phases and is punctuated by a distinct period of contraction. The spreading duration and peak contact areas are independent of the surface opsonin density, although the opsonin density does affect the likelihood that a cell will spread...
December 20, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27997605/role-of-myeloperoxidase-oxidants-in-the-modulation-of-cellular-lysosomal-enzyme-function-a-contributing-factor-to-macrophage-dysfunction-in-atherosclerosis
#8
Fahd O Ismael, Tessa J Barrett, Diba Sheipouri, Bronwyn E Brown, Michael J Davies, Clare L Hawkins
Low-density lipoprotein (LDL) is the major source of lipid within atherosclerotic lesions. Myeloperoxidase (MPO) is present in lesions and forms the reactive oxidants hypochlorous acid (HOCl) and hypothiocyanous acid (HOSCN). These oxidants modify LDL and have been strongly linked with the development of atherosclerosis. In this study, we examined the effect of HOCl, HOSCN and LDL pre-treated with these oxidants on the function of lysosomal enzymes responsible for protein catabolism and lipid hydrolysis in murine macrophage-like J774A...
2016: PloS One
https://www.readbyqxmd.com/read/27938439/the-antileishmanial-activity-of-xanthohumol-is-mediated-by-mitochondrial-inhibition
#9
Lianet Monzote, Alexandra Lackova, Katrin Staniek, Silvia Steinbauer, Gerald Pichler, Walter Jäger, Lars Gille
Xanthohumol (Xan) is a natural constituent of human nutrition. Little is known about its actions on leishmanial parasites and their mitochondria as putative target. Therefore, we determined the antileishmanial activity of Xan and resveratrol (Res, as alternative compound with antileishmanial activity) with respect to mitochondria in Leishmania amazonensis promastigotes/amastigotes (LaP/LaA) in comparison with their activity in peritoneal macrophages from mouse (PMM) and macrophage cell line J774A.1 (J774). Mechanistic studies were conducted in Leishmania tarentolae promastigotes (LtP) and mitochondrial fractions isolated from LtP...
December 12, 2016: Parasitology
https://www.readbyqxmd.com/read/27906031/contrasting-biological-potency-of-particulate-matter-collected-at-sites-impacted-by-distinct-industrial-sources
#10
Errol M Thomson, Dalibor Breznan, Subramanian Karthikeyan, Christine MacKinnon-Roy, Ngoc Q Vuong, Ewa Dabek-Zlotorzynska, Valbona Celo, Jean-Pierre Charland, Prem Kumarathasan, Jeffrey R Brook, Renaud Vincent
BACKGROUND: Industrial sources contribute a significant proportion of anthropogenic particulate matter (PM) emissions, producing particles of varying composition that may differentially impact health. This study investigated the in vitro toxicity of ambient PM collected near industrial sites in relation to particle size and composition. METHODS: Size-fractionated particles (ultrafine, PM0.1-2.5, PM2.5-10, PM>10) were collected in the vicinity of steel, copper, aluminium, and petrochemical industrial sites...
December 1, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27876275/development-of-leishmania-donovani-stably-expressing-dsred-for-flow-cytometry-based-drug-screening-using-chalcone-thiazolyl-hydrazone-as-a-new-antileishmanial-target
#11
Anil Kumar Jaiswal, K Bhaskara Rao, Pragati Kushwaha, Keerti Rawat, Ram K Modukuri, Prashant Khare, Sumit Joshi, Shikha Mishra, Ambak Rai, Koneni V Sashidhara, Anuradha Dube
Green fluorescent protein produces significant fluorescence and is extremely stable, however its excitation maximum is close to the ultraviolet range and thus can damage living cells. Hence, Leishmania donovani stably expressing DsRed were developed and their suitability for flow cytometry-based antileishmanial screening was assessed by evaluating the efficacies of standard drugs as well as newly synthesised chalcone thiazolyl-hydrazone compounds. The DsRed gene was successfully integrated at the 18S rRNA locus of L...
December 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27876107/sodium-butyrate-protects-mice-from-the-development-of-the-early-signs-of-non-alcoholic-fatty-liver-disease-role-of-melatonin-and-lipid-peroxidation
#12
Cheng Jun Jin, Anna Janina Engstler, Cathrin Sellmann, Doreen Ziegenhardt, Marianne Landmann, Giridhar Kanuri, Hakima Lounis, Markus Schröder, Walter Vetter, Ina Bergheim
Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases worldwide with universally accepted treatments still lacking. Oral supplementation of sodium butyrate (SoB) has been suggested to attenuate liver damage of various aetiologies. Our study aimed to further delineate mechanisms involved in the SoB-dependent hepatic protection using a mouse model of fructose-induced NAFLD and in in vitro models. C57BL/6J mice were either pair-fed a fructose-enriched liquid diet ±0·6 g/kg body weight per d SoB or standard chow for 6 weeks...
November 23, 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/27856731/airway-delivery-of-interferon-%C3%AE-overexpressing-macrophages-confers-resistance-to-mycobacterium-avium-infection-in-scid-mice
#13
Rajamouli Pasula, Bradley E Britigan, Banurekha Kesavalu, Maher Y Abdalla, William J Martin
Mycobacterium avium (M. avium) causes significant pulmonary infection, especially in immunocompromised hosts. Alveolar macrophages (AMs) represent the first line of host defense against infection in the lung. Interferon gamma (IFN-γ) activation of AMs enhances in vitro killing of pathogens such as M. avium We hypothesized that airway delivery of AMs into the lungs of immunodeficient mice infected with M. avium will inhibit M. avium growth in the lung and that this macrophage function is in part IFN-γ dependent...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27812336/piperine-suppresses-pyroptosis-and-interleukin-1%C3%AE-release-upon-atp-triggering-and-bacterial-infection
#14
Yi-Dan Liang, Wen-Jing Bai, Chen-Guang Li, Li-Hui Xu, Hong-Xia Wei, Hao Pan, Xian-Hui He, Dong-Yun Ouyang
Piperine is a phytochemical present in black pepper (Piper nigrum Linn) and other related herbs, possessing a wide array of pharmacological activities including anti-inflammatory effects. Previously, we demonstrated that piperine has therapeutic effects on bacterial sepsis in mice, but the underlying mechanism has not been fully elucidated. In this study, we aimed to investigate the influences of piperine on pyroptosis in murine macrophages. The results showed that piperine dose-dependently inhibited ATP-induced pyroptosis, thereby suppressing interleukin-1β (IL-1β) or high mobility group box-1 protein (HMGB1) release in LPS-primed bone marrow-derived macrophages and J774A...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27769937/secretome-profiling-of-highly-virulent-mycobacterium-bovis-04-303-strain-reveals-higher-abundance-of-virulence-associated-proteins
#15
Fernando Vargas-Romero, Guillermo Mendoza-Hernández, Francisco Suárez-Güemes, Rogelio Hernández-Pando, Mauricio Castañón-Arreola
Mycobacterium bovis is the causative agent of tuberculosis in farms, wildlife and causes sporadic disease in humans. Despite the high similitude in genome sequence between M. bovis strains, some strains like the wild boar 04-303 isolate show a highly virulent phenotype in animal models. Comparative studies will contribute to link protein expression with the virulence phenotype. In vitro, the 04-303 strain was more phagocytized by J774A.1 macrophages in comparison with 444 strain (a cow isolate with the same genotype) and BCG...
November 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27696135/acrolein-increases-macrophage-atherogenicity-in-association-with-gut-microbiota-remodeling-in-atherosclerotic-mice-protective-role-for-the-polyphenol-rich-pomegranate-juice
#16
Oren Rom, Hila Korach-Rechtman, Tony Hayek, Yael Danin-Poleg, Haim Bar, Yechezkel Kashi, Michael Aviram
The unsaturated aldehyde acrolein is pro-atherogenic, and the polyphenol-rich pomegranate juice (PJ), known for its anti-oxidative/anti-atherogenic properties, inhibits macrophage foam cell formation, the hallmark feature of early atherosclerosis. This study aimed to investigate two unexplored areas of acrolein atherogenicity: macrophage lipid metabolism and the gut microbiota composition. The protective effects of PJ against acrolein atherogenicity were also evaluated. Atherosclerotic apolipoprotein E-deficient (apoE(-/-)) mice that were fed acrolein (3 mg/kg/day) for 1 month showed significant increases in serum and aortic cholesterol, triglycerides, and lipid peroxides...
September 30, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27620851/n-acetyl-tryptophan-glucopyranoside-natg-as-a-countermeasure-against-gamma-radiation-induced-immunosuppression-in-murine-macrophage-j774a-1-cells
#17
Poonam Malhotra, Manish Adhikari, Saurabh Mishra, Sumit Singh, Piyush Kumar, Shravan Kumar Singh, Raj Kumar
Radiation exposure to immune system induces imbalance in cytokines expression involved in Th1/Th2 homeostasis perturbations. In the present study, N-acetyl tryptophan glucoside (NATG), a bacterial secondary metabolite, was evaluated for its possible radioprotective potential to immune system using J774A.1 murine macrophages. In this study, expression of IFN-γ, TNF-α, IL-10, IL-2, IL-12, IL-13 and IL-17A cytokines was analyzed in irradiated and NATG pretreated cells using ELISA assay. Results of the study indicated that irradiated macrophages (NK-1R(+ )cells) pretreated with NATG showed higher (p < ...
2016: Free Radical Research
https://www.readbyqxmd.com/read/27544994/effect-of-15-deoxy-%C3%A2-12-14-prostaglandin-j2-on-expression-of-macrophage-migration-inhibitory-factor-in-mouse-monocyte-macrophage-cell-line-j774a-1
#18
L I Wei-Yang, Shi Yu-Meng, Liu Xin, Yang Lin, L I Li-Ying
Objective To investigate the effect of 15-Deoxy-△(12,14)-prostaglandin J2 (15 d-PGJ2) on the expression of macrophage migration inhibitory factor (MIF) and its underlying mechanism in J774A.1. Methods The murine monocyte/macrophage cell line J774A.1 were divided into six groups:lipopolysaccharide (LPS) group,incubated with 1 μg/ml LPS for 1 h;normal control group,incubated with PBS for 1 h;negative control group,incubated with 5 μmol/L 15 d-PGJ2 for 1 h;15 d-PGJ2 group,incubated with 5 μmol/L 15 d-PGJ2 for 1 h followed by 1 μg/ml LPS for 1 h;GW9662 group,incubated with 5 μmol/L 15 d-PGJ2 for 1 h following GW9662 10 μmol/L for 1 h,and then incubated with 1 μg/ml LPS for 1 h;and Vehicle group,control of GW9662,GW9662 was replaced by its solvent DMSO...
June 10, 2016: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/27520027/development-of-an-integrated-approach-for-comparison-of-in-vitro-and-in-vivo-responses-to-particulate-matter
#19
Dalibor Breznan, Subramanian Karthikeyan, Marcelle Phaneuf, Prem Kumarathasan, Sabit Cakmak, Michael S Denison, Jeffrey R Brook, Renaud Vincent
BACKGROUND: Association of particulate matter with adverse health effects has been established in epidemiological studies and animal experiments. Epidemiological studies are difficult to undertake while animal studies are impractical for high-throughput toxicity testing. The ease and rapidity of in vitro tests emphasizes their potential for use in risk assessment of chemicals and particles. We examined the association between in vitro and in vivo responses to ambient particles, to determine the potential of cell-based assays as standalone toxicity screening tools...
August 12, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27517171/supplementation-with-linoleic-acid-rich-soybean-oil-stimulates-macrophage-foam-cell-formation-via-increased-oxidative-stress-and-diacylglycerol-acyltransferase1-mediated-triglyceride-biosynthesis
#20
Oren Rom, Helana Jeries, Tony Hayek, Michael Aviram
During the last decades there has been a staggering rise in human consumption of soybean oil (SO) and its major polyunsaturated fatty acid linoleic acid (LA). The role of SO or LA in cardiovascular diseases is highly controversial, and their impact on macrophage foam cell formation, the hallmark of early atherogenesis, is unclear. To investigate the effects of high SO or LA intake on macrophage lipid metabolism and the related mechanisms of action, C57BL/6 mice were orally supplemented with increasing levels of SO-based emulsion or equivalent levels of purified LA for 1 month, followed by analyses of lipid accumulation and peroxidation in aortas, serum and in peritoneal macrophages (MPM) of the mice...
August 12, 2016: BioFactors
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