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J774a.1

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https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-pqs-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#1
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonas aeruginosa (PA) causes lung infection in patients with cystic fibrosis (CF). Pseudomonas Quinolone Signal (PQS) is a secreted PA virulence factor that contributes to the pathogenicity of PA. We were able to detect PQS in sputum samples from CF patients infected with PA, but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial (NHBE)) and macrophages (J774A...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28621735/anti-inflammatory-and-anti-oxidant-potential-of-the-root-extract-and-constituents-of-doronicum-austriacum
#2
Stefania Marzocco, Simona Adesso, Mostafa Alilou, Hermann Stuppner, Stefan Schwaiger
Doronicum austriacum Jacq., Asteraceae, is a plant which is used in traditional alpine medicine. Historical sources describe the medical use of the root, but up until now only a few studies evaluated its pharmacological properties. The evaluation of the dichloromethane extract, and its major compounds for their anti-inflammatory and anti-oxidant potential was performed in macrophages J774A.1 and C6 astrocytes. Nitric oxide (NO) and reactive oxygen species (ROS) release, as well as nitrotyrosine formation, were evaluated...
June 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28608692/galactomannan-from-antrodia-cinnamomea-enhances-the-phagocytic-activity-of-macrophages
#3
Namal Perera, Feng-Ling Yang, Ching-Ming Chang, Yueh-Tung Lu, Shih-Hao Zhan, Yi-Ting Tsai, Jung-Feng Hsieh, Lan-Hui Li, Kuo-Feng Hua, Shih-Hsiung Wu
Galactomannan with an octasaccharide-repeating unit (ACP) was isolated from Taiwan medicinal mushroom Antrodia cinnamomea, and its chemical structure was determined herein. ACP significantly enhanced the phagocytosis and bactericidal activity of J774A.1 murine macrophages against Escherichia coli, with prospects for developing a new immunomodulatory compound or adjuvant in immunotherapy and vaccination.
June 13, 2017: Organic Letters
https://www.readbyqxmd.com/read/28605335/in-vitro-expression-of-toll-like-receptors-and-proinflammatory-molecules-induced-by-ergosta-7-22-dien-3-one-isolated-from-a-wild-mexican-strain-of-ganoderma-oerstedii-agaricomycetes
#4
Angel Ramos-Ligonio, Aracely López-Monteon, Maria de la Soledad Lagunes-Castro, Jorge Suarez-Medellin, Cesar Espinoza, Guillermo Mendoza, Angel Trigos
Compounds showing pharmacological activity on the immune system are of interest because of their therapeutic potential in the treatment of many diseases. However, data from primary human immune cells and in vivo studies are limited. The aim of this study was to analyze the ability to induce the expression of Toll-like receptors (TLRs) and proinflammatory molecules on cells involved in the immune system using the compound ergosta-7,22-dien-3- one, isolated from a wild Mexican strain of Ganoderma oerstedii. According to our study, ergosta-7,22-dien-3-one did not present any cytotoxic effect on HeLa or J774A...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28600163/involvement-of-tyrosine-specific-protein-kinase-and-protein-kinase-c-in-j774a-1-macrophage-functions-activated-by-tinospora-cordifoliain%C3%A2-vitro
#5
Priti More, Kalpana Pai
BACKGROUND: Macrophages are the first line of defense and constitute important participant in the bi-directional interaction between innate and specific immunity. Macrophages are in a quiescent form and get activated when given a stimulus. In our previous studies we have reported that guduchi or LPS treatment of macrophages enhanced production of nitric oxide (NO) and increased tumoricidal activity against L929 fibroblast cells. OBJECTIVE: In the present study effect of Tinospora cordifolia commonly known as guduchi on macrophage activation and the mechanism of action i...
June 6, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/28582854/activation-of-macrophages-and-microglia-by-interferon-%C3%AE-and-lipopolysaccharide-increases-methylglyoxal-production-a-new-mechanism-in-the-development-of-vascular-complications-and-cognitive-decline-in-type-2-diabetes-mellitus
#6
Karthik Dhananjayan, Dhanushka Gunawardena, Nerissa Hearn, Tanja Sonntag, Chris Moran, Erika Gyengesi, Velandai Srikanth, Gerald Münch
Methylglyoxal (MGO), a dicarbonyl compound derived from glucose, is elevated in diabetes mellitus and contributes to vascular complications by crosslinking collagen and increasing arterial stiffness. It is known that MGO contributes to inflammation as it forms advanced glycation end products (AGEs), which activate macrophages via the receptor RAGE. The aim of study was to investigate whether inflammatory activation can increase MGO levels, thereby completing a vicious cycle. In order to validate this, macrophage (RAW264...
May 30, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28575082/the-role-of-reactive-oxygen-intermediates-in-the-intracellular-fate-of-leptospira-interrogans-in-the-macrophages-of-different-hosts
#7
Shijun Li, Peili Li, Lei Zhang, Weilin Hu, Ming Wang, Ying Liu, Guangpeng Tang, Dingming Wang, Bijun Zhou, Jie Yan
BACKGROUND: Pathogenic species of Leptospira cause leptospirosis, a global zoonotic disease. Our previous work showed that leptospires survive and replicate in human macrophages but are killed in murine macrophages. However, the mechanism responsible for the different intracellular fates of leptospires within the macrophages of different hosts remains unclear. RESULTS: The present study demonstrates that infection with Leptospira interrogans caused significant up-regulation of reactive oxygen species (ROS) and superoxide in J774A...
2017: PloS One
https://www.readbyqxmd.com/read/28527905/peroxisome-proliferator-activated-receptor-%C3%AE-mediated-polarization-of-macrophages-in-neospora-caninum-infection
#8
Xuexiu He, Pengtao Gong, Zhengkai Wei, Weijian Liu, Weili Wang, Jianhua Li, Zhengtao Yang, Xichen Zhang
BACKGROUND: Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear...
July 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28431321/atherogenicity-of-amino-acids-in-the-lipid-laden-macrophage-model-system-in-vitro-and-in-atherosclerotic-mice-a-key-role-for-triglyceride-metabolism
#9
Oren Rom, Claudia Grajeda-Iglesias, Mahmoud Najjar, Niroz Abu-Saleh, Nina Volkova, Dalit Esther Dar, Tony Hayek, Michael Aviram
Atherosclerosis-related research has focused mainly on the effects of lipids on macrophage foam cell formation and atherogenesis, whereas the role of amino acids (AAs) was understudied. The current study aimed to identify anti- or pro-atherogenic AA in the macrophage model system and to elucidate the underlying metabolic and molecular mechanisms. J774A.1 cultured macrophages were treated with increasing concentrations of each 1 of the 20 AAs. Macrophage atherogenicity was assessed in terms of cellular toxicity, generation of reactive oxygen species (ROS) and cellular cholesterol or triglyceride content...
April 6, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28420125/bioavailable-citrus-sinensis-extract-polyphenolic-composition-and-biological-activity
#10
Giacomo Pepe, Francesco Pagano, Simona Adesso, Eduardo Sommella, Carmine Ostacolo, Michele Manfra, Marcello Chieppa, Marina Sala, Mariateresa Russo, Stefania Marzocco, Pietro Campiglia
Citrus plants contain large amounts of flavonoids with beneficial effects on human health. In the present study, the antioxidant and anti-inflammatory potential of bioavailable polyphenols from Citrus sinensis was evaluated in vitro and ex vivo, using the murine macrophages cell line J774A.1 and primary peritoneal macrophages. Following simulated gastro-intestinal digestion, the in vitro bioavailability of Citrus sinensis polyphenolic extract was assessed using the human cell line Caco-2 grown as monolayers on a transwell membrane...
April 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28419526/soluble-egg-antigen-activates-m2-macrophages-via-the-stat6-and-pi3k-pathways-and-schistosoma-japonicum-alternatively-activates-macrophage-polarization-to-improve-the-survival-rate-of-septic-mice
#11
Hao Tang, Yan-Bing Liang, Zhi-Bin Chen, Lin-Lin Du, Li-Jin Zeng, Jing-Guo Wu, Wen Yang, Hua-Ping Liang, Zhong-Fu Ma
Sepsis is one of the most challenging health problems worldwide. Our previous study showed that chronic schistosoma japonica (SJ) infection might increase serum anti-inflammatory factors to play a protective role, thus improving the survival rate of septic mice. Further research revealed that SJ infection promoted J774A.1 macrophage differentiation into M2 macrophages; suppressed LPS-induced activation of M1 macrophages; up-regulated CD163, IL-10, and TGF-β1 expression; inhibited TNF-α and iNOS expression; and blocked the effect of LPS-promoted TNF-α and iNOS expression...
April 17, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28398240/anti-inflammatory-antioxidant-and-crystallographic-studies-of-n-palmitoyl-ethanol-amine-pea-derivatives
#12
Carmela Saturnino, Ada Popolo, Anna Ramunno, Simona Adesso, Michela Pecoraro, Maria Rosaria Plutino, Silvia Rizzato, Alberto Albinati, Stefania Marzocco, Marina Sala, Domenico Iacopetta, Maria Stefania Sinicropi
N-Palmitoyl-ethanolamine (PEA) is an anti-inflammatory component of egg yolk that is usually employed for the prevention of respiratory apparatus virus infection and then frequently used for its efficient anti-inflammatory and analgesic effects in experimental models of visceral, neuropathic, and inflammatory diseases. Nevertheless, data of its use in animal or human therapy are still scarce and further studies are needed. Herein, we report the biological evaluation of a small library of N-palmitoyl-ethanolamine analogues or derivatives, characterized by a protected acid function (either as palmitoyl amides or hexadecyl esters), useful to decrease their hydrolysis rate in vitro and prolong their biological activity...
April 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28394618/potential-eye-drop-based-on-a-calix-4-arene-nanoassembly-for-curcumin-delivery-enhanced-drug-solubility-stability-and-anti-inflammatory-effect
#13
Giuseppe Granata, Irene Paterniti, Corrada Geraci, Francesca Cunsolo, Emanuela Esposito, Marika Cordaro, Anna Rita Blanco, Salvatore Cuzzocrea, Grazia M L Consoli
Curcumin is an Indian spice with a wide spectrum of biological and pharmacological activities but poor aqueous solubility, rapid degradation, and low bioavailability that affect medical benefits. To overcome these limits in ophthalmic application, curcumin was entrapped in a polycationic calix[4]arene-based nanoaggregate by a simple and reproducible method. The calix[4]arene-curcumin supramolecular assembly (Calix-Cur) appeared as a clear colloidal solution consisting in micellar nanoaggregates with size, polydispersity index, surface potential, and drug loading percentage meeting the requirements for an ocular drug delivery system...
April 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28368124/development-of-surface-variable-polymeric-nanoparticles-for-drug-delivery-to-tumors
#14
Ning Han, Liang Pang, Jun Xu, Hyesun Hyun, Jinho Park, Yoon Yeo
To develop nanoparticle drug carriers that interact with cells specifically in the mildly acidic tumor microenvironment, we produced polymeric nanoparticles modified with amidated TAT peptide via a simple surface modification method. Two types of core poly(lactic-co-glycolic acid) nanoparticles (NL and NP) were prepared with a phospholipid shell as an optional feature and covered with polydopamine that enabled the conjugation of TAT peptide on the surface. Subsequent treatment with acid anhydrides such as cis-aconitic anhydride (CA) and succinic anhydride (SA) converted amines of lysine residues in TAT peptide to β-carboxylic amides, introducing carboxylic groups that undergo pH-dependent protonation and deprotonation...
April 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28365379/phosphotransferase-systems-in-enterococcus-faecalis-og1rf-enhance-anti-stress-capacity-in%C3%A2-vitro-and-in%C3%A2-vivo
#15
Zhen Peng, Matthias A Ehrmann, Anna Waldhuber, Christine Niemeyer, Thomas Miethke, Julia-Stefanie Frick, Tao Xiong, Rudi F Vogel
Phosphotransferase systems are common and essential in bacteria, which are in charge of sugar transportation and phosphorylation. However, phosphotransferase systems were found in recent years to be associated with environmental stress factors. This study investigated the role of the mannose/fructose/sorbose phosphotransferase systems in Enterococcus faecalis OG1RF in adaption to harsh environments by construction of pts mutants. More than one mannose/fructose/sorbose phosphotransferase system was found in E...
March 29, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28361953/cell-to-cell-transfer-of-saa1-protein-in-a-cell-culture-model-of-systemic-aa-amyloidosis
#16
Stephanie Claus, Ioana Puscalau-Girtu, Paul Walther, Tatiana Syrovets, Thomas Simmet, Christian Haupt, Marcus Fändrich
Systemic AA amyloidosis arises from the misfolding of serum amyloid A1 (SAA1) protein and the deposition of AA amyloid fibrils at multiple sites within the body. Previous research already established that mononuclear phagocytes are crucial for the formation of the deposits in vivo and exposure of cultures of such cells to SAA1 protein induces the formation of amyloid deposits within the culture dish. In this study we show that both non-fibrillar and fibrillar SAA1 protein can be readily transferred between cultured J774A...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300196/recombinant-mycobacterium-smegmatis-with-a-pmyong2-vector-expressing-human-immunodeficiency-virus-type-i-gag-can-induce-enhanced-virus-specific-immune-responses
#17
Byoung-Jun Kim, Jeong-Ryeol Gong, Ga-Na Kim, Bo-Ram Kim, So-Young Lee, Yoon-Hoh Kook, Bum-Joon Kim
Recently, we have developed a novel Mycobacterium-Escherichia coli shuttle vector system using pMyong2, which can provide an enhanced expression of heterologous genes in recombinant Mycobacterium smegmatis (rSmeg). To investigate the usefulness of rSmeg using pMyong2 in vaccine application, we vaccinated M. smegmatis with pMyong2 system expressing Human Immunodeficiency Virus Type I (HIV-1) Gag p24 antigen (rSmeg-pMyong2-p24) into mice and examined its cellular and humoral immune responses against HIV gag protein...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28275134/the-complement-anaphylatoxins-c5a-and-c3a-suppress-ifn-%C3%AE-production-in-response-to-listeria-monocytogenes-by-inhibition-of-the-cyclic-dinucleotide-activated-cytosolic-surveillance-pathway
#18
Stacey L Mueller-Ortiz, Daniel G Calame, Nancy Shenoi, Yi-Dong Li, Rick A Wetsel
Listeria monocytogenes is an intracellular Gram-positive bacterium that induces expression of type I IFNs (IFN-α/IFN-β) during infection. These cytokines are detrimental to the host during infection by priming leukocytes to undergo L. monocytogenes-mediated apoptosis. Our previous studies showed that C5aR1(-/-) and C3aR(-/-) mice are highly susceptible to L. monocytogenes infection as a result of increased IFN-β-mediated apoptosis of major leukocyte cell populations, including CD4(+) and CD8(+) T cells. However, the mechanisms by which C3a and C5a modulate IFN-β expression during L...
April 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28268194/a-bioluminescent-caspase-1-activity-assay-rapidly-monitors-inflammasome-activation-in-cells
#19
Martha O'Brien, Danielle Moehring, Raúl Muñoz-Planillo, Gabriel Núñez, Justin Callaway, Jenny Ting, Mike Scurria, Tim Ugo, Laurent Bernad, James Cali, Dan Lazar
Inflammasomes are protein complexes induced by diverse inflammatory stimuli that activate caspase-1, resulting in the processing and release of cytokines, IL-1β and IL-18, and pyroptosis, an immunogenic form of cell death. To provide a homogeneous method for detecting caspase-1 activity, we developed a bioluminescent, plate-based assay that combines a substrate, Z-WEHD-aminoluciferin, with a thermostable luciferase in an optimized lytic reagent added directly to cultured cells. Assay specificity for caspase-1 is conferred by inclusion of a proteasome inhibitor in the lytic reagent and by use of a caspase-1 inhibitor to confirm activity...
March 6, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28237876/passive-transfer-of-interferon-%C3%AE-over-expressing-macrophages-enhances-resistance-of-scid-mice-to-mycobacterium-tuberculosis-infection
#20
Rajamouli Pasula, William J Martin, Banu Rekha Kesavalu, Maher Y Abdalla, Bradley E Britigan
Infection with Mycobacterium tuberculosis (M.tb) is associated with increased deaths worldwide. Alveolar macrophages (AMs) play a critical role in host defense against infection with this pathogen. In this work we tested the hypothesis that passive transfer of normal AMs, IFN-γ activated AMs, or macrophages transduced to over-express IFN-γ into the lungs of immunosuppressed SCID mice, where resident macrophages are present but not functional, would enhance alveolar immunity and increase clearance of pulmonary M...
February 23, 2017: Cytokine
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