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https://www.readbyqxmd.com/read/29133045/synthesis-characterization-and-antileishmanial-activity-of-neutral-n-heterocyclic-carbenes-gold-i-complexes
#1
Chen Zhang, Sandra Bourgeade Delmas, Álvaro Fernández Álvarez, Alexis Valentin, Catherine Hemmert, Heinz Gornitzka
A series of five new mononuclear neutral gold(I) complexes containing N-heterocyclic carbenes (NHCs) was synthesized and fully characterized by spectroscopic methods. The X-ray structures of four complexes are presented. These gold(I) complexes together with four other neutral gold(I)-NHC complexes previously described were evaluated in vitro against Leishmania infantum promastigotes and axenic amastigotes. Moreover, their cytotoxicity was assessed on the murine macrophages J774A.1. Except one complex (10), eight gold(I)-NHC-Cl complexes show potent activity against the pathological relevant form of L...
October 24, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29128095/olive-oil-polyphenols-extracts-inhibit-inflammatory-markers-in-j774a-1-murine-macrophages-and-scavenge-free-radicals
#2
Marwa Abdallah, Stefania Marzocco, Simona Adesso, Mokhtar Zarrouk, Mokhtar Guerfel
Here we evaluate the olive oil antiradical and anti-inflammatory potential through its polyphenols extracts and examine the influence of olive maturity on olive oil quality properties, polyphenols composition and biological potentials. Samples have been obtained from minor Tunisian olive cultivars (Chemchali, Fouji and Zarrazi) at different maturity indices. Principal quality properties were evaluated and polyphenols analysis was carried out by Folin Ciocalteu reagent and HPLC-UV-MS. Antiradical activity was examined by DPPH and FRAP scavenging assays while J774A...
November 8, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29076425/cytotoxic-effects-and-production-of-cytokines-induced-by-the-endophytic-paenibacillus-polymyxa-rnc-d-in-vitro
#3
Débora Meira Neris, Genoveva Lourdes Flores Luna, Joice Margareth de Almeida Rodolpho, Ana Carolina Urbaczek, Ricardo de Oliveira Correia, Karina Alves Feitosa, Nadja Fernanda Gonzaga Serrano, Cristina Paiva de Sousa, Fernanda de Freitas Anibal
BACKGROUND: Prominent among all the organisms that have a potential value for the pro-duction of new medicines, are endophytes, fungi and bacteria that live inside plants without harming them. In this study, a total lyophilized extract (TLE) of Paenibacillus polymyxa RNC-D was used. The P. polymyxa lineages are known for their capacity to segregate a large number of extracellular enzymes and bioactive substances. METHODS: The TLE of Paenibacillus polymyxa RNC-D was tested in cell viability assays for cytotoxicity and cytokine production in BALB/3T3 and J774A...
October 26, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29042278/a-ribonuclease-inhibitor-resistant-dimer-of-human-pancreatic-ribonuclease-displays-specific-antitumor-activity
#4
Ayush Attery, Punyatirtha Dey, Prajna Tripathi, Janendra K Batra
Human pancreatic ribonuclease (HPR) and bovine seminal ribonuclease (BS-RNase) are members of the RNase A superfamily. HPR is monomeric, whereas BS-RNase is dimeric. BS-RNase has strong antitumor and cytotoxic activities. However, HPR lacks cytotoxic activity as it is inactivated by intracellular cytosolic ribonuclease inhibitor (RI). Earlier, an RI-resistant cytotoxic variant of HPR, termed HPR-KNE was generated which contained three residues Lys7, Asn71 and Glu111 of HPR, known to interact with RI, mutated to alanine...
October 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29033558/effect-of-surface-organic-coatings-of-cellulose-nanocrystals-on-the-viability-of-mammalian-cell-lines
#5
Ambar S Jimenez, Francesca Jaramillo, Usha D Hemraz, Yaman Boluk, Karina Ckless, Rajesh Sunasee
Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification of CNCs continues to gain significant research interest as it imparts new properties to the surface of the nanocrystals for the design of multifunctional CNCs-based materials. A small chemical surface modification can potentially lead to drastic behavioral changes of cell-material interactions thereby affecting the intended bio-application. In this work, unmodified CNCs were covalently decorated with four different organic moieties such as a diaminobutane fragment, a cyclic oligosaccharide (β-cyclodextrin), a thermoresponsive polymer (poly[N-isopropylacrylamide]), and a cationic aminomethacrylamide-based polymer using different synthetic covalent methods...
2017: Nanotechnology, Science and Applications
https://www.readbyqxmd.com/read/29031243/preparation-and-evaluation-of-rifampicin-and-co-trimoxazole-loaded-nanocarrier-against-brucella-melitensis-infection
#6
Narges Bodaghabadi, Samira Hajigholami, Ziba Vaise Malekshahi, Maliheh Entezari, Farhood Najafi, Hadi Shirzad, Majid Sadeghizadeh
Background: Brucellosis or Malta fever is a contagious infection common between human and domestic animals. Many antibiotics are used for brucellosis treatment, but they are not efficient and put heavy burden on society. Co-trimoxazole and rifampicin are two candidates for brucellosis treatment. In this study, we aimed to enhance the efficacy of these antibiotics using designed nanoparticles. Methods: Different concentrations of co-trimoxazole and rifampicin were used for loading onto a nanostructure of synthesized monomethoxy poly(ethylene glycol)-oleate (mPEG-OA)...
October 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28928657/colocynth-extracts-prevent-epithelial-to-mesenchymal-transition-and-stemness-of-breast-cancer-cells
#7
Kaushik Chowdhury, Ankit Sharma, Suresh Kumar, Gyanesh K Gunjan, Alo Nag, Chandi C Mandal
Modern treatment strategies provide better overall survival in cancer patients, primarily by controlling tumor growth. However, off-target and systemic toxicity, tumor recurrence, and resistance to therapy are still inadvertent hurdles in current treatment regimens. Similarly, metastasis is another deadly threat to patients suffering from cancer. This has created an urgent demand to come up with new drugs having anti-metastatic potential and minimum side effects. Thus, this study was aimed at exploring the anti-proliferative and anti-metastatic potential of colocynth medicinal plant...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28822264/trimethylamine-n-oxide-as-a-risk-factor-for-atherosclerosis-induces-stress-in-j774a-1-murine-macrophages
#8
Abbas Mohammadi, Zakaria Vahabzadeh, Soran Jamalzadeh, Tahereh Khalili
PURPOSE: Trimethylamine N-oxide (TMAO) is a biomarker for kidney problems. It has also been introduced as a risk factor for atherosclerosis. The classic risk factors for atherosclerosis trigger cellular and humeral immunoreaction in macrophages through induction of heat shock protein expressions and increased levels of GRP94 and HSP70 are associated with increased atherosclerosis risk. The present study evaluated the possible effect(s) of TMAO on the expression of GRP94 and HSP70 at protein levels...
August 16, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28791964/effect-of-copper-on-the-up-regulation-down-regulation-of-genes-cytotoxicity-and-ion-dissolution-for-mesoporous-bioactive-glasses
#9
Gurbinder Kaur, N Sriranganathan, S G Waldrop, Purnima Sharma, B N Chudasama
In the present study, copper-based (25 - x)CaO - xCuO -10P2O5 - 5B2O3 - 60SiO2 (x = 2.5, 5, 7.5, 10 mol%) mesoporous bioactive glasses (MBGs) were synthesized using the sol-gel technique with cetyltrimethyl ammonium bromide as the structure-directing agent. The live-dead cell count and cytocompatibility of MBGs for J774A.1 murine macrophages have also been investigated for different concentrations of MBGs. The ionic dissolution profile for Ca, P and Si has been evaluated in the simulated body fluid. The effect of copper content as well as the ionic dissolution products on the up-regulation and down-regulation of TGIF-2, HDAC-4, Smurf-1, mir-30c and mir-130a genes for the murine model are investigated using reverse transcription-polymerase chain reaction...
August 9, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28791016/doxycycline-attenuates-leptospira-induced-il-1%C3%AE-by-suppressing-nlrp3-inflammasome-priming
#10
Wenlong Zhang, Xufeng Xie, Dianjun Wu, Xuemin Jin, Runxia Liu, Xiaoyu Hu, Yunhe Fu, Zhuang Ding, Naisheng Zhang, Yongguo Cao
Doxycycline (Dox), a semisynthetic antibiotic, has been reported to exert multiple immunomodulatory effects. Treatment with Dox has a satisfactory curative effect against leptospirosis. In addition to its antibacterial action, we supposed that Dox also modulated immune response in controlling leptospira infection. Using J774A.1 mouse macrophages, the effects of Dox on protein and mRNA levels of IL-1β and TNF-α were investigated after infection with live or sonicated Leptospira interrogans serovar Lai strain Lai (56601)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28770171/ul36-rescues-apoptosis-inhibition-and-in-vivo-replication-of-a-chimeric-mcmv-lacking-the-m36-gene
#11
M Zeeshan Chaudhry, Bahram Kasmapour, Carlos Plaza-Sirvent, Milica Bajagic, Rosaely Casalegno Garduño, Lisa Borkner, Tihana Lenac Roviš, Andrea Scrima, Stipan Jonjic, Ingo Schmitz, Luka Cicin-Sain
Apoptosis is an important defense mechanism mounted by the immune system to control virus replication. Hence, cytomegaloviruses (CMV) evolved and acquired numerous anti-apoptotic genes. The product of the human CMV (HCMV) UL36 gene, pUL36 (also known as vICA), binds to pro-caspase-8, thus inhibiting death-receptor apoptosis and enabling viral replication in differentiated THP-1 cells. In vivo studies of the function of HCMV genes are severely limited due to the strict host specificity of cytomegaloviruses, but CMV orthologues that co-evolved with other species allow the experimental study of CMV biology in vivo...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28766178/differential-regulation-of-il-1%C3%AE-and-il-6-release-in-murine-macrophages
#12
Seishiro Hirano, Quan Zhou, Akiko Furuyama, Sanae Kanno
Asbestos and silica (exogenous danger) and adenosine triphosphate (ATP, endogenous danger-signaling molecule) synergistically increase IL-1β release from endotoxin-primed macrophage, which is mediated by NOD-like receptor protein 3 (NLRP3) inflammasome. However, the conversion of pro-IL-1β to its active form seems to depend on the macrophage cell types. In the present study, bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1β and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1β from pro-IL-1β...
December 2017: Inflammation
https://www.readbyqxmd.com/read/28737923/cosilencing-intestinal-transglutaminase-2-and-interleukin-15-using-gelatin-based-nanoparticles-in-an-in-vitro-model-of-celiac-disease
#13
Husain Attarwala, Valerie Clausen, Prasoon Chaturvedi, Mansoor M Amiji
In this study, we have developed a type B gelatin nanoparticle based siRNA delivery system for silencing of intestinal transglutaminase-2 (TG2) and interleukin-15 (IL-15) genes in cultured human intestinal epithelial cells (Caco-2) and murine alveolar macrophage cells (J774A.1). Small interfering RNA (siRNA) targeting the TG2 or IL-15 gene was encapsulated within gelatin nanoparticles using ethanol-water solvent displacement method. Size, charge, and morphology of gelatin nanoparticles were evaluated using a Zetasizer instrument and transmission electron microscopy...
August 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28716750/hexadecylphosphocholine-miltefosine-stabilized-chitosan-modified-ampholipospheres-as-prototype-co-delivery-vehicle-for-enhanced-killing-of-l-donovani
#14
Priyanka Tripathi, Anil Kumar Jaiswal, Anuradha Dube, Prabhat Ranjan Mishra
Lipid nanoparticles are stable, biodegradable and biocompatible carriers offering excellent therapeutic efficacy. Here, a novel effort has been made to develop Miltefosine (HePC- hexadecylphosphocholine) stabilized chitosan anchored nanostructured lipid carriers (NLC) of Amphotericin B (AmB) as co-delivery vehicle to enhance killing of L. donovani. The entrapment efficiency of AmB was achieved upto 85.3% for HePC-AmB-CNLCs with mean particle size of 150.8±8.4nm, and zeta potential value of +28.2±1.1mV, respectively...
December 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28669709/a-mechanistic-insight-into-curcumin-modulation-of-the-il-1%C3%AE-secretion-and-nlrp3-s-glutathionylation-induced-by-needle-like-cationic-cellulose-nanocrystals-in-myeloid-cells
#15
Andrew Guglielmo, Adham Sabra, Mostafa Elbery, Milena M Cerveira, Fernanda Ghenov, Rajesh Sunasee, Karina Ckless
Recently we have demonstrated that needle-like cationic cellulose nanocrystals (CNC-AEMA2) evoke immunological responses through NLRP3 inflammasome/IL-1β inflammatory pathway. In this study we demonstrated that curcumin, a naturally occurring polyphenolic compound isolated from Curcuma longa (Zingiberaceae), was able to suppress, at least in part, this immunological response, as observed by diminished IL-1β secretion in CNC-AEMA2-stimulated macrophages primed with LPS. Curcumin is a well-known antioxidant and anti-inflammatory natural compound and in addition to acting as "scavenger" of reactive oxygen species (ROS), it can also upregulates antioxidant enzymes...
August 25, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28666415/soluble-factors-from-biofilm-of-candida-albicans-and-staphylococcus-aureus-promote-cell-death-and-inflammatory-response
#16
Kassia de Carvalho Dias, Paula Aboud Barbugli, Fernanda de Patto, Virginia Barreto Lordello, Letícia de Aquino Penteado, Alexandra Ivo Medeiros, Carlos Eduardo Vergani
BACKGROUND: The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species Candida albicans (C. albicans) and methicillin-sensitive Staphylococcus aureus (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of C. albicans and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT)...
June 30, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28653829/development-and-characterization-of-a-hydroxyl-sulfonamide-analogue-5-chloro-n-2-4-hydroxysulfamoyl-phenyl-ethyl-2-methoxy-benzamide-as-a-novel-nlrp3-inflammasome-inhibitor-for-potential-treatment-of-multiple-sclerosis
#17
Chunqing Guo, Jacob W Fulp, Yuqi Jiang, Xia Li, Jeremy E Chojnacki, Jingde Wu, Xiang-Yang Wang, Shijun Zhang
In our efforts to develop novel small-molecule inhibitors for the NOD-like receptor family pyrin-domain-containing 3 (NLRP3) inflammasome as potential disease-modifying agents to treat neurological disorders including multiple sclerosis (MS), a hydroxyl sulfonamide analogue JC-171 has been rationally designed and biologically characterized both in vitro and in vivo. Our studies established that JC-171 dose dependently inhibited LPS/ATP-induced interleukin-1β (IL-1β) release from J774A.1 macrophages with an IC50 of 8...
October 18, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28651168/the-antioxidant-and-anti-inflammatory-properties-of-lycopene-in-mice-lungs-exposed-to-cigarette-smoke
#18
Keila Karine Duarte Campos, Glaucy Rodrigues Araújo, Thais Lourenço Martins, Ana Carla Balthar Bandeira, Guilherme de Paula Costa, André Talvani, Camila Carrião Machado Garcia, Laser Antônio Machado Oliveira, Daniela Caldeira Costa, Frank Silva Bezerra
Lycopene is a carotenoid with known antioxidant and anti-inflammatory properties. We aimed to evaluate the in vitro and in vivo effects of lycopene on reducing the redox imbalance and inflammation induced by cigarette smoke (CS). For the in vitro study, J774A.1 (macrophages) cells were incubated in the presence of 0.5, 1.0, 2.0, 4.0, 8.0, 10.0 and 25 μM of lycopene for 3, 6 and 24 h or in the presence of 0.1%, 0.25%, 0.5%, 0.625%, 1.25%, 2.25%, 5% and 10% cigarette smoke extract (CSE) for 3, 6 and 24 h to assess cell viability and measurement of intracellular reactive oxygen species (ROS)...
October 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#19
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonasaeruginosa causes lung infections in patients with cystic fibrosis (CF). The Pseudomonas quinolone signal (PQS) compound is a secreted P. aeruginosa virulence factor that contributes to the pathogenicity of P. aeruginosa We were able to detect PQS in sputum samples from CF patients infected with P. aeruginosa but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce the production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial [NHBE]) cells and macrophages (J774A...
September 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28621735/anti-inflammatory-and-anti-oxidant-potential-of-the-root-extract-and-constituents-of-doronicum-austriacum
#20
Stefania Marzocco, Simona Adesso, Mostafa Alilou, Hermann Stuppner, Stefan Schwaiger
Doronicum austriacum Jacq., Asteraceae, is a plant which is used in traditional alpine medicine. Historical sources describe the medical use of the root, but up until now only a few studies evaluated its pharmacological properties. The evaluation of the dichloromethane extract, and its major compounds for their anti-inflammatory and anti-oxidant potential was performed in macrophages J774A.1 and C6 astrocytes. Nitric oxide (NO) and reactive oxygen species (ROS) release, as well as nitrotyrosine formation, were evaluated...
June 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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