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Monocyte cell culture

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https://www.readbyqxmd.com/read/28812210/increased-il-6-expression-on-thp-1-by-il-34-stimulation-up-regulated-rheumatoid-arthritis-th17-cells
#1
Bing Wang, Yawei Tang, Xiaotong Sun, Xunli Ouyang, Han Li, Jing Wei, Yan Zhang, Xia Li
IL-34 is a pleiotropic cytokine, which is a key regulator of monocytes/macrophages and might participate in the pathogenesis of RA. In this study, we aimed to explore the effect of IL-34 on the monocyte-like cell line THP-1 and the quantitative variation of Th17 cells in THP-1 and RA CD4(+)T cells coculture system. CD4(+)T cells were purified from RA PBMC using immunomagnetic beads. THP-1 were cultured with RA CD4(+)T cells. The frequency of Th17 cells was determined by FACS. Fluorscence indensity and expression of ROS were detected by FACS and cell staining, respectively...
August 15, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28810630/selective-depletion-of-cultured-macrophages-by-magnetite-nanoparticles-modified-with-gelatin
#2
Yoshihiro Komohara, Ryuta Kawauchi, Erika Makiyama, Kazuki Mikami, Hasita Horlad, Yukio Fujiwara, Tetsuya Kida, Motohiro Takeya, Takuro Niidome
Previous studies have indicated pro-tumor functions of macrophages in tumor progression in different types of malignant tumors. The detailed mechanisms of cell-cell interaction between macrophages and tumor cells have been investigated by means of in vitro co-culture experiments. The present study developed magnetite nanoparticles modified with gelatin that are specifically engulfed by macrophages and investigated methods to deplete these macrophages in co-culture experiments using a magnet. T98G glioma cell line and human monocyte-derived macrophages were mixed and co-cultured for 2 days...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28805137/differential-influences-of-fc-gamma-receptor-blocking-on-the-effects-of-certolizumab-pegol-and-infliximab-on-human-monocytes
#3
Takayuki Hoshiyama, Yu Matsueda, Toshihiro Tono, Yoshiyuki Arinuma, Tatsuo Nagai, Shunsei Hirohata
OBJECTIVES: To compare the effects of certolizumab pegol (CZP) and infliximab (IFX) on human monocytes. METHODS: Highly purified monocytes from healthy donors were cultured with CZP, IFX, control IgG1, or polyethylene glycol (PEG) at pharmacological attainable concentrations in culture medium with 10% autologous normal human serum (NHS) or with fetal bovine serum (FBS) for 24 h, after which the supernatants were replaced by fresh culture medium containing LPS...
August 14, 2017: Modern Rheumatology
https://www.readbyqxmd.com/read/28805019/cd11b-gr1-dim-tolerogenic-dendritic-cell-like-cells-are-expanded-in-interstitial-lung-disease-in-skg-mice
#4
Sho Sendo, Jun Saegusa, Takaichi Okano, Soshi Takahashi, Kengo Akashi, Akio Morinobu
OBJECTIVE: SKG mice develop interstitial lung disease (ILD) resembling rheumatoid arthritis-associated ILD (RA-ILD) in human. The aim of this study is to clarify the mechanism behind the lung pathology by analyzing the lung-infiltrating cells in ILD of SKG mice. METHODS: We assessed the severity of zymosan A (ZyA)-induced ILD in SKG mice histologically, and examined lung-infiltrating cells by flow cytometry. Total lung cells and isolated monocytic myeloid-derived suppressor cells (M-MDSCs) were cultured in vitro with GM-CSF (and IL-4)...
August 13, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28803951/monocytes-inhibit-hepatitis-c-virus-induced-trail-expression-on-cd56-bright-nk-cells
#5
Dalila Mele, Stefania Mantovani, Barbara Oliviero, Giulia Grossi, Andrea Lombardi, Mario U Mondelli, Stefania Varchetta
BACKGROUND AND AIMS: Natural killer (NK) cells play an important role in the pathogenesis of HCV infection. We have previously shown that culture-derived HCV (HCVcc) enhance tumor necrosis-factor-related apoptosis-inducing ligand (TRAIL) expression on healthy but not on HCV-infected patient-derived NK cells, which was likely dependent on accessory cells. Here we sought mechanistic insights involved in altered TRAIL upregulation in this setting. METHODS: Peripheral blood mononuclear cells (PBMC) from controls and HCV-infected patients were exposed to HCVcc...
August 10, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28802127/investigations-on-the-molecular-mode-of-action-of-the-novel-immunostimulator-zelnate-activation-of-the-cgas-sting-pathway-in-mammalian-cells
#6
Thomas Ilg
Bovine respiratory disease (BRD) is usually prevented or treated with vaccines and/or antibiotics. The use of antibiotics is, however, of concern due to the potential promotion of microbial resistance and the occurrence of residues. Recently an alternative aid in the treatment of BRD, the cationic lipid/bacterial plasmid DNA liposome-based immunomodulator ZelNate, has entered the veterinary market. In the present study, we provide data on the molecular mode of action of ZelNate. Despite the presence of numerous non-methylated CpG motifs in its plasmid DNA, ZelNate proved to be inactive on human and mouse toll-like receptor 9 (TLR9) in cell culture, in both recombinant and natural cellular receptor settings...
August 9, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28802000/pmepa1-induced-by-rankl-p38-mapk-pathway-has-a-novel-role-in-osteoclastogenesis
#7
Noboru Funakubo, Xianghe Xu, Toshio Kukita, Seiji Nakamura, Hiroshi Miyamoto, Akiko Kukita
Osteoclasts are multinucleated cells formed by fusion of preosteoclasts (POCs) derived from cells of the monocyte/macrophage lineage. We have reported a culture system that supports the formation of POCs from stroma-depleted rat bone marrow cells. Global gene expression analysis of this culture system identified genes highly expressed in POCs. Here, we have analyzed the expression and function of one of these highly expressed genes, prostate transmembrane protein androgen induced 1 (Pmepa1), a target of TGF-β and binds Nedd4 ubiquitin ligase, which plays a role in intracellular trafficking...
August 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28801363/l-selectin-promotes-the-maturation-of-dendritic-cells-via-up-regulation-the-expression-of-tlr4-in-vitro
#8
Zhishuai Ye, Jia Liu, Jie Zheng, Jianing Zhang, Rongchong Huang
The relationship between dendritic cells (DCs) and L-selectin in the progress of atherosclerosis is unclear. Here, we used L-selectin co-cultured with DCs to investigate the effect of L-selectin on the maturation of DCs in vitro Monocytes derived DCs were isolated and cultured from human peripheral blood. After being stimulated with L-selectin and/or its antagonist for 24-48 hours, the feather of cells was observed by the electron microscope. The expression of mature antigens CD1a, CD80, CD83, and CD86 were investigated by flow cytometric analysis (FACS)...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28800844/first-comparative-analysis-concerning-the-plasma-platelet-contamination-during-mnc-collection
#9
Hella Pfeiffer, Susanne Achenbach, Julian Strobel, Robert Zimmermann, Reinhold Eckstein, Erwin F Strasser
BACKGROUND AND OBJECTIVES: Monocytes can be cultured into dendritic cells with addition of autologous plasma, which is highly prone to platelet contamination due to the apheresis process. Since platelets affect the maturation process of monocytes into dendritic cells and might even lead to a diminished harvest of dendritic cells, it is very important to reduce the platelet contamination. A new collection device (Spectra Optia) was analyzed, compared to two established devices (COM.TEC, Cobe Spectra) and evaluated regarding the potential generation of source plasma...
July 13, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28799643/sebocytes-contribute-to-skin-inflammation-by-promoting-the-differentiation-of-th17-cells
#10
M Mattii, M Lovászi, N Garzorz, A Atenhan, M Quaranta, F Lauffer, A Konstantinow, M Küpper, C C Zouboulis, L Kemeny, K Eyerich, C B Schmidt-Weber, D Törőcsik, S Eyerich
BACKGROUND: The main function of sebocytes is considered to be the lipid production for moisturizing the skin. However, it became recently apparent that sebocytes release chemokines and cytokines and respond to pro-inflammatory stimuli as well as presence of bacteria. OBJECTIVES: To analyze the functional communication between human sebocytes and T cells. METHODS: Immunofluorescence stainings for CD4 and IL-17 were performed on acne sections and healthy skin...
August 11, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28794041/interferon-inducible-cd169-siglec1-attenuates-anti-hiv-1-effects-of-ifn-%C3%AE
#11
Hisashi Akiyama, Nora-Guadalupe Pina Ramirez, Gregory Gibson, Christopher Kline, Simon Watkins, Zandrea Ambrose, Suryaram Gummuluru
A hallmark of HIV-1 infection in vivo is chronic immune activation concomitant with type I interferon (IFN) production. Although type I IFN induces an antiviral state in many cell types, HIV-1 can replicate in vivo via mechanisms that have remained unclear. We have recently identified a type I IFN-inducible protein CD169 as the HIV-1 attachment factor on dendritic cells (DCs) that can mediate robust infection of CD4(+) T cells in trans. Since CD169 expression on macrophages is also induced by type I IFN, we hypothesized that type I IFN-inducible CD169 could facilitate productive HIV-1 infection in myeloid cells in cis and CD4(+) T cells in trans and thus offset antiviral effects of type I IFN...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28794002/klf4-regulation-of-ch25h-and-lxr-mitigates-atherosclerosis-susceptibility
#12
Zhao Li, Marcy Martin, Jin Zhang, Hsi-Yuan Huang, Liang Bai, Jiao Zhang, Jian Kang, Ming He, Jie Li, Mano R Maurya, Shakti Gupta, Guangjin Zhou, Panjamaporn Sangwung, Yong-Jiang Xu, Ting Lei, Hsien-Da Huang, Mohit Jain, Mukesh K Jain, Shankar Subramaniam, John Y-J Shyy
Background -Atherosclerosis is a multifaceted inflammatory disease involving cells in the vascular wall [e.g., endothelial cells (ECs)] as well as circulating and resident immunogenic cells (e.g., monocytes/macrophages). Acting as a ligand for liver X receptor (LXR), but an inhibitor of sterol regulatory element binding protein 2 (SREBP2), 25-hydroxycholesterol (25-HC) and its catalyzing enzyme cholesterol-25-hydroxylase (Ch25h) are important in regulating cellular inflammatory status and cholesterol biosynthesis in both ECs and monocytes/macrophages...
August 9, 2017: Circulation
https://www.readbyqxmd.com/read/28791439/metabolic-crosstalk-between-fatty-pancreas-and-fatty-liver-effects-on-local-inflammation-and-insulin-secretion
#13
Felicia Gerst, Robert Wagner, Gabriele Kaiser, Madhura Panse, Martin Heni, Jürgen Machann, Malte N Bongers, Tina Sartorius, Bence Sipos, Falko Fend, Christian Thiel, Silvio Nadalin, Alfred Königsrainer, Norbert Stefan, Andreas Fritsche, Hans-Ulrich Häring, Susanne Ullrich, Dorothea Siegel-Axel
AIMS/HYPOTHESIS: Obesity-linked ectopic fat accumulation is associated with the development of type 2 diabetes. Whether pancreatic and liver steatosis impairs insulin secretion is controversial. We examined the crosstalk of human pancreatic fat cells with islets and the role of diabetogenic factors, i.e. palmitate and fetuin-A, a hepatokine released from fatty liver. METHODS: Human pancreatic resections were immunohistochemically stained for insulin, glucagon, somatostatin and the macrophage/monocyte marker CD68...
August 8, 2017: Diabetologia
https://www.readbyqxmd.com/read/28791015/influence-of-irradiated-peripheral-blood-mononuclear-cells-on-both-ex-vivo-proliferation-of-human-natural-killer-cells-and-change-in-cellular-property
#14
María Delso-Vallejo, Jutta Kollet, Ulrike Koehl, Volker Huppert
Clinical studies with adoptive immunotherapy using allogeneic natural killer (NK) cells showed feasibility, but also limitation regarding the transfused absolute cell numbers. First promising results with peripheral blood mononuclear cells (PBMCs) as feeder cells to improve the final cell number need further optimization and investigation of the unknown controlling mechanism in the cross-talk to NK cells. We investigated the influence of irradiated autologous PBMCs to boost NK cell proliferation in the presence of OKT3 and IL-2...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28783866/hematologic-and-plasma-chemistry-ris-for-cultured-striped-catfish-pangasius-hypophthalmus-in-recirculating-aquaculture-systems
#15
Oscar A Galagarza, David D Kuhn, Stephen A Smith, Terry C Hrubec
BACKGROUND: Striped catfish (Pangasius hypophthalmus) is a valuable aquaculture fish species produced primarily in Southeast Asia. In the United States, it is bred as an ornamental species. Striped catfish has high productivity and great demand in numerous countries around the world, yet little is known about its normal physiology. OBJECTIVES: The objective of this study was to establish hematologic and blood chemistry RIs for healthy juvenile Striped catfish. METHODS: Blood samples were collected from 70 Striped catfish raised in recirculating aquaculture systems...
August 7, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28774105/strontium-substituted-bioceramics-particles-a-new-way-to-modulate-mcp-1-and-gro-%C3%AE-production-by-human-primary-osteoblastic-cells
#16
Julien Braux, Frédéric Velard, Christine Guillaume, Marie-Laure Jourdain, Sophie C Gangloff, Edouard Jallot, Jean-Marie Nedelec, Patrice Laquerrière, Dominique Laurent-Maquin
BACKGROUND: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines' secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles...
December 5, 2016: Materials
https://www.readbyqxmd.com/read/28771838/human-regulatory-macrophages-are-potent-in-suppression-of-the-xenoimmune-response-via-indoleamine-2-3-dioxygenase-involved-mechanism-s
#17
Fei Guo, Min Hu, Dandan Huang, Yuanfei Zhao, Benjamin Heng, Gilles Guillemin, Chai K Lim, Wayne J Hawthorne, Shounan Yi
BACKGROUND: For xenotransplantation to truly succeed, we must develop immunomodulatory strategies to suppress the xenoimmune response but by minimizing immunosuppression over the long term. Regulatory macrophages (Mreg) have been shown to suppress polyclonal T-cell proliferation in vitro and prolong allograft survival in vivo. However, the question of whether they are capable of suppressing xenoimmune responses remains unknown. This study assessed the potential of human Mreg to be used as an effective immunomodulatory method in xenotransplantation...
August 2, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28765923/bombyx-mori-hemocyte-extract-has-anti-inflammatory-effects-on-human-phorbol-myristate-acetate-differentiated-thp%C3%A2-1-cells-via-tlr4-mediated-suppression-of-the-nf-%C3%AE%C2%BAb-signaling-pathway
#18
Young Il Kim, Kwang Ho Choi, Seong Ryul Kim, Tae-Won Goo, Seung-Won Park
Hemolymph is the circulating fluid of insects and is a key component of their immune system. However, little is known concerning hemocyte identification, development, differentiation and related cellular immune responses. The present study aimed to determine whether a hemocyte extract prepared from Bombyx mori larvae had anti‑inflammatory effects; THP‑1 (a human monocytic leukemia cell line) cells that had been differentiated into macrophage‑like cells by treatment with phorbol myristate acetate (PMA) were used...
July 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28764923/association-of-rs1285933-single-nucleotide-polymorphism-in-clec5a-gene-with-dengue-severity-and-its-functional-effects
#19
Caroline Xavier-Carvalho, Renata Duarte da Silva Cezar, Naishe Matos Freire, Carla Mola, Victor Fiesta, Thiago Gomes de Toledo-Pinto, Luciana Gomes Fialho Torres, Rodrigo Feliciano do Carmo, Luydson Richardson Silva Vasconcelos, Marli Tenório Cordeiro, Paulo Baptista, Elzinandes Leal de Azeredo, Rivaldo Venâncio da Cunha, Luiz José de Souza, Antonio Guilherme Pacheco, Claire Fernandes Kubelka, Patrícia Moura, Milton Ozorio Moraes
Outbreaks of the Zika, dengue, and chikungunya viruses, especially in the Americas, pose a global threat due to their rapid spread and difficulty controlling the vector. Extreme phenotypes are often observed, from asymptomatic to severe clinical manifestations, which are well-studied in dengue. Host variations are also important contributors to disease outcomes, and many case-control studies have associated single nucleotide polymorphisms (SNPs) with severe dengue. Here, we found that the TC genotype and T-carriers for SNP rs1285933 in the C-type lectin superfamily member 5 (CLEC5A) gene was associated with severe dengue in a Northern Brazilian population (OR=2...
July 29, 2017: Human Immunology
https://www.readbyqxmd.com/read/28763032/a-chip-for-estrogen-receptor-action-detection-of-biomarkers-released-by-mcf-7-cells-through-estrogenic-and-anti-estrogenic-effects
#20
Konstanze Gier, Claudia Preininger, Ursula Sauer
The fluorescence-based multi-analyte chip platform for the analysis of estrogenic and anti-estrogenic substances is a new in vitro tool for the high throughput screening of environmental samples. In contrast to existing tools, the chip investigates the complex action of xenoestrogens in a human cell model by characterizing protein expression. It allows for the quantification of 10 proteins secreted by MCF-7 cells, representing various biological and pathological endpoints of endocrine action and distinguishing between estrogen- and anti-estrogen-dependent secretion of proteins...
August 1, 2017: Sensors
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