keyword
MENU ▼
Read by QxMD icon Read
search

Monocyte cell culture

keyword
https://www.readbyqxmd.com/read/29156538/combined-effects-of-simulated-microgravity-and-radiation-exposure-on-osteoclast-cell-fusion
#1
Srinivasan Shanmugarajan, Ye Zhang, Maria Moreno-Villanueva, Ryan Clanton, Larry H Rohde, Govindarajan T Ramesh, Jean D Sibonga, Honglu Wu
The loss of bone mass and alteration in bone physiology during space flight are one of the major health risks for astronauts. Although the lack of weight bearing in microgravity is considered a risk factor for bone loss and possible osteoporosis, organisms living in space are also exposed to cosmic radiation and other environmental stress factors. As such, it is still unclear as to whether and by how much radiation exposure contributes to bone loss during space travel, and whether the effects of microgravity and radiation exposure are additive or synergistic...
November 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29149667/tumor-acidosis-and-hypoxia-differently-modulate-the-inflammatory-program-measurements-in-vitro-and-in-vivo
#2
Anne Riemann, Sarah Reime, Oliver Thews
Inflammatory mediators produced by the tumor cells are of importance for immune response but also for malignant progression. The aim of the study was to analyze the expression of monocyte chemoattractant protein-1, interleukin-6 (IL-6), tumor necrosis factor-α, inducible isoform of nitric oxide synthase (iNOS), cyclooxygenase-2, and osteopontin in vitro in two different tumor cell lines under hypoxia (pO2≈1.5 mmHg) and/or acidosis (pH=6.6) for up to 24 hours since hypoxia and acidosis are common characteristics of solid tumors...
November 14, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29149648/dried-plum-alleviates-symptoms-of-inflammatory-arthritis-in-tnf-transgenic-mice
#3
Faryal Mirza, Joseph Lorenzo, Hicham Drissi, Francis Y Lee, Do Y Soung
Dried plum (DP), a rich source of polyphenols has been shown to have bone-preserving properties in both animal models of osteoporosis and postmenopausal women. We evaluated if DP alleviated the destruction of joints in transgenic mice (TG) that overexpress human tumor necrosis factor (TNF), a genetic model of rheumatoid arthritis (RA). A four-week treatment of 20% DP diet in TG slowed the onset of arthritis and reduced bone erosions in the joints compared to TG on a regular diet. This was associated with fewer tartrate-resistant acid phosphatase (TRAP) positive cells, suggesting decreased osteoclastogenesis...
October 16, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29146966/revisiting-the-role-of-interleukin-8-in-chronic-lymphocytic-leukemia
#4
Denise Risnik, Enrique Podaza, María B Almejún, Ana Colado, Esteban E Elías, Raimundo F Bezares, Horacio Fernández-Grecco, Santiago Cranco, Julio C Sánchez-Ávalos, Mercedes Borge, Romina Gamberale, Mirta Giordano
The proliferation and survival of malignant B cells in chronic lymphocytic leukemia (CLL) depend on signals from the microenvironment in lymphoid tissues. Among a plethora of soluble factors, IL-8 has been considered one of the most relevant to support CLL B cell progression in an autocrine fashion, even though the expression of IL-8 receptors, CXCR1 and CXCR2, on leukemic B cells has not been reported. Here we show that circulating CLL B cells neither express CXCR1 or CXCR2 nor they respond to exogenous IL-8 when cultured in vitro alone or in the presence of monocytes/nurse-like cells...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145829/effect-of-a-rare-genetic-variant-of-tm7sf4-gene-on-osteoclasts-of-patients-with-paget-s-disease-of-bone
#5
Emilie Laurier, Nathalie Amiable, Edith Gagnon, Jacques P Brown, Laëtitia Michou
BACKGROUND: Dendritic Cell-Specific Transmembrane Protein (DC-STAMP) is involved in osteoclastogenesis with a key role in mononucleated osteoclasts fusion. We reported in patients with Paget's disease of bone (PDB) a rare variant (rs62620995) in the TM7SF4 gene, encoding for DC-STAMP, which changes a highly conserved amino acid, possibly damaging according to in silico predictions. This study aimed at determining the functional effects of this variant on osteoclast phenotype in PDB. METHODS: Fifty ml of peripheral blood were collected in pagetic patients carrier of this variant (n = 4) or not (n = 4) and healthy controls (n = 4)...
November 16, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/29145789/helicobacter-pylori-controls-nlrp3-expression-by-regulating-hsa-mir-223-3p-and-il-10-in-cultured-and-primary-human-immune-cells
#6
Suneesh Kumar Pachathundikandi, Steffen Backert
Inflammasome-mediated production of mature IL-1β and IL-18 cytokines represents an important innate immune response against infecting pathogens. Helicobacter pylori, one of the most successful and persistent human pathogens, induces severe inflammation leading to gastritis and more serious gastric diseases. H. pylori modulates different immune responses for its survival and inflammasome signaling is manipulated by the cag pathogenicity island ( cagPAI), urease and VacA cytotoxin. Here we report that H. pylori regulates NLRP3 expression, an inflammasome forming regulator, in infected THP-1 monocytes...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29141644/lipopolysaccharide-pretreatment-increases-protease-activated-receptor-2-expression-and-monocyte-chemoattractant-protein-1-secretion-in-vascular-endothelial-cells
#7
Hung-Hsing Chao, Po-Yuan Chen, Wen-Rui Hao, Wei-Ping Chiang, Tzu-Hurng Cheng, Shih-Hurng Loh, Yuk-Man Leung, Ju-Chi Liu, Jin-Jer Chen, Li-Chin Sung
BACKGROUND: This study investigated whether lipopolysaccharide (LPS) increase protease-activated receptor-2 (PAR-2) expression and enhance the association between PAR-2 expression and chemokine production in human vascular endothelial cells (ECs). METHODS: The morphology of ECs was observed through microphotography in cultured human umbilical vein ECs (EA. hy926 cells) treated with various LPS concentrations (0, 0.25, 0.5, 1, and 2 μg/mL) for 24 h, and cell viability was assessed using the MTT assay...
November 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29141050/differential-immunomodulation-in-human-monocytes-versus-macrophages-by-filarial-cystatin
#8
Gopinath Venugopal, Marion Mueller, Susanne Hartmann, Svenja Steinfelder
Parasitic nematodes have evolved powerful immunomodulatory molecules to enable their survival in immunocompetent hosts by subverting immune responses and minimizing pathological processes. One filarial molecule known to counteract host immune responses by inducing IL-10 and regulatory macrophages in mice is filarial cystatin. During a patent filarial infection monocytes encounter microfilariae in the blood, an event that occurs in asymptomatically infected filariasis patients that are immunologically hyporeactive...
2017: PloS One
https://www.readbyqxmd.com/read/29137267/tumacrophage-macrophages-transformed-into-tumor-stem-like-cells-by-virulent-genetic-material-from-tumor-cells
#9
Yizhuang Zhang, Na Zhou, Xiuyan Yu, Xuehui Zhang, Shanxin Li, Zhen Lei, Ruobi Hu, Hui Li, Yiqing Mao, Xi Wang, Jinshu Zhang, Yuan Li, Hongyan Guo, David M Irwin, Gang Niu, Huanran Tan
Tumor-associated macrophages are regarded as tumor-enhancers as they have key roles in the subversion of adaptive immunity and in inflammatory circuits that promote tumor progression. Here, we show that cancer cells can subvert macrophages yielding cells that have gained pro-tumor functions. When macrophages isolated from mice or humans are co-cultured with dead cancer cell line cells, induced to undergo apoptosis to mimic chemotherapy, up-regulation of pro-tumor gene expression was identified. Phagocytosis of apoptotic cancer cells by macrophages resulted in their transformation into tumor stem (initiating)-like cells, as indicated by the expression of epithelial markers (e...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134442/endothelial-tnf-%C3%AE-induction-by-hsp60-secreted-from-thp-1-monocytes-exposed-to-hyperglycaemic-conditions
#10
Ryan Dennis Martinus, Julie Goldsbury
A non-resolving inflammation of the endothelium is recognised to be an important process leading to atherosclerosis. In diabetes, this process is thought to account for a significant number of cardiovascular disease-associated death and disability. However, the molecular mechanisms by which diabetes contributes to endothelial inflammation remain to be established. Whilst there is some evidence linking hyperglycaemia-induced reactive oxygen species (ROS) formation by the mitochondrial electron-transport chain to oxidative stress, cellular injury and apoptosis in the endothelium, a clear link to endothelium inflammation has not yet been established...
November 13, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29133194/effect-of-native-and-minimally-modified-low-density-lipoprotein-on-the-activation-of-monocyte-subsets
#11
Francisco Blanco-Favela, José Esteban Espinosa-Luna, Adriana Karina Chávez-Rueda, Alejandra Madrid-Miller, Luis Chávez-Sánchez
BACKGROUND: In atherosclerosis, monocytes are essential and secrete pro-inflammatory cytokines in response to modified low-density lipoprotein (LDL). Human CD14(++)CD16(-), CD14(++)CD16(+) and CD14(+)CD16(++) monocytes produce different cytokines. The objective of this research was to determine the number of monocyte subsets positives to cytokines in response to native (nLDL) and minimally modified LDL (mmLDL). METHODS: Human monocytes from healthy individuals were purified by negative selection and were stimulated with nLDL, mmLDL or LPS...
November 10, 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/29132256/mcp-1-produced-by-keratinocytes-is-associated-with-leucocyte-recruitment-during-elicitation-of-nickel-induced-occupational-allergic-contact-dermatitis
#12
Weihua Mai, Dongqing Lu, Xingwei Liu, Ling Chen
To investigate the expression profile of monocyte chemoattractant peptide-1 (MCP-1) by keratinocytes after nickel exposure and to identify its role for leucocyte migration during nickel-induced occupational allergic contact dermatitis (OACD), 26 workers diagnosed with nickel-induced OACD were enrolled. Skin biopsies from the positive nickel-challenged sites at different time points were assessed by immunohistochemistry (IHC) for MCP-1, CD68, CD45RO, and in situ hybridization (ISH) for MCP-1, using chronic periumbilical dermititis as controls...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/29128662/activation-of-innate-immunity-in-primary-human-cells-using-a-plant-virus-derived-nanoparticle-tlr7-8-agonist
#13
Damien Carignan, Sabine Herblot, Marie-Ève Laliberté-Gagné, Marilène Bolduc, Michel Duval, Pierre Savard, Denis Leclerc
Rod-shaped virus-like nanoparticles (VLNP) made of papaya mosaic virus (PapMV) coat proteins (CP) self-assembled around a single stranded RNA (ssRNA) were showed to be a TLR7 agonist. Their utilization as an immune modulator in cancer immunotherapy was showed promising. To establish a clinical relevance in human for PapMV VLNP, we showed that stimulation of human peripheral blood mononuclear cells (PBMC) with VLNP induces the secretion of interferon alpha (IFNα) and other pro-inflammatory cytokines and chemokines...
November 8, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29127410/ultrafiltration-combined-with-size-exclusion-chromatography-efficiently-isolates-extracellular-vesicles-from-cell-culture-media-for-compositional-and-functional-studies
#14
Birke J Benedikter, Freek G Bouwman, Tanja Vajen, Alexandra C A Heinzmann, Gert Grauls, Edwin C Mariman, Emiel F M Wouters, Paul H Savelkoul, Carmen Lopez-Iglesias, Rory R Koenen, Gernot G U Rohde, Frank R M Stassen
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies. Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29126435/human-amnion-cells-reverse-acute-and-chronic-pulmonary-damage-in-experimental-neonatal-lung-injury
#15
Dandan Zhu, Jean Tan, Amina S Maleken, Ruth Muljadi, Siow T Chan, Sin N Lau, Kirstin Elgass, Bryan Leaw, Joanne Mockler, Daniel Chambers, Kristen T Leeman, Carla F Kim, Euan M Wallace, Rebecca Lim
BACKGROUND: Despite advances in neonatal care, bronchopulmonary dysplasia (BPD) remains a significant contributor to infant mortality and morbidity. While human amnion epithelial cells (hAECs) have shown promise in small and large animal models of BPD, there is scarce information on long-term benefit and clinically relevant questions surrounding administration strategy remain unanswered. In assessing the therapeutic potential of hAECs, we investigated the impact of cell dosage, administration routes and timing of treatment in a pre-clinical model of BPD...
November 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29122211/citrate-dialysate-does-not-induce-oxidative-stress-or-inflammation-in-vitro-as-compared-to-acetate-dialysate
#16
Rafael Pérez-García, Rafael Ramírez Chamond, Patricia de Sequera Ortiz, Marta Albalate, Marta Puerta Carretero, Mayra Ortega, M Caridad Ruiz Caro, Roberto Alcazar Arroyo
Increased acetataemia during haemodialysis sessions has been associated with a number of abnormalities, including increased oxidative stress, pro-inflammatory cytokines and nitric oxide synthesis. However, citric acid may play an alternative role to acetate as a dialysate stabiliser given that the effect of citrate on complement and leukocyte activation is different to that of acetate. The purpose of this study was to compare the inflammatory effect in immunocompetent blood cells of acetate dialysate and citrate dialysate...
November 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
https://www.readbyqxmd.com/read/29122013/suppression-of-methylmercury-induced-mip-2-expression-by-n-acetyl-l-cysteine-in-murine-raw264-7-macrophage-cell-line
#17
Juliet David, Athira Nandakumar, Muflihatul Muniroh, Suminori Akiba, Megumi Yamamoto, Chihaya Koriyama
BACKGROUND: The aim of this study is to examine the inflammatory-cytokine expressions in the presence of non-cytotoxic dose of methylmercury (MeHg) in murine macrophages, which is suspected to play an important role in brain damage caused by MeHg exposure. We focused on murine macrophage inflammatory protein-2 (MIP-2), keratinocyte chemoattractant (KC), and monocyte chemoattractant protein-5 (MCP-5). MIP-2 and KC are murine functional homologues of human IL-8 and MCP-5 for human MCP-1...
November 9, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/29118012/effects-and-mechanisms-of-caffeine-to-improve-immunologic-and-metabolic-abnormalities-in-diet-induced-obese-rats
#18
Chih-Wei Liu, Hung-Cheng Tsai, Chia-Chang Huang, Chang-Youh Tsai, Yen-Bo Su, Ming-Wei Lin, Kuei Chuan Lee, Yun-Cheng Hsieh, Tzu-Hao Li, Shiang-Fen Huang, Ying-Ying Yang, Ming-Chih Hou, Han-Chieh Lin, Fa-Yauh Lee, Shou-Dong Lee
In obesity, there are no effective therapies for parallel immune and metabolic abnormalities including systemic/tissue insulin-resistance/inflammation, adiposity and hepatic steatosis. Caffeine has anti-inflammation, anti-hepatic steatosis and anti-insulin-resistance effects. In this study, we evaluated the effects and molecular mechanisms of 6-week of caffeine treatment (HFD-caf) on immunologic and metabolic abnormalities of high-fat diet (HFD)-induced obese rats. In comparison with HFD-V rats, in HFD-caf rats the suppressed circulating immune cell inflammatory [TNFα, MCP-1, IL-6, intercellular adhesion molecule1 (ICAM-1) and nitrite] profiles were accompanied by decreased liver, white adipose tissue (WAT) and muscle' macrophages and its intracellular cytokines levels...
November 7, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29116252/leptin-regulates-granzyme-a-pd-1-and-ctla-4-expression-in-t-cell-to-control-visceral-leishmaniasis-in-balb-c-mice
#19
Alti Dayakar, Sambamurthy Chandrasekaran, Jalaja Veronica, Vadloori Bharadwaja, Radheshyam Maurya
Visceral leishmaniasis (VL) is responsible for several deaths in malnourished children accompanied by diminished circulating leptin and impaired cell-mediated immunity. Typically, leptin deficiency is associated with the Th2 polarization that markedly coincides with the pathogenesis of VL. The aim of the present study was to unravel the prophylactic role of leptin in malnutrition-coupled VL mice. Interestingly, we observed that L. donovani infection itself reduces the serum leptin levels in malnutrition. Exogenous leptin restored severe body weight loss and parasite load in the spleen and liver of malnourished infected mice compared to controls...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115433/adiponectin-promotes-preadipocyte-differentiation-via-the-ppar%C3%AE-pathway
#20
Wenkai Yang, Chunli Yang, Jun Luo, Yutao Wei, Wenying Wang, Yingmei Zhong
According to the results of a preliminary study, it was hypothesized that the effects of adiponectin (APN) on the improvement of atherosclerosis may be associated with adipocyte differentiation and peroxisome proliferator‑activated receptor γ (PPARγ). The present study simulated the inflammatory environment of epicardial adipose tissue by stimulating mature adipocytes with lipopolysaccharide (LPS); subsequently, the differentiation of 3T3‑L1 preadipocytes was observed. 3T3‑L1 preadipocytes were infected with an adenovirus containing the human adiponectin gene apM1 (Ad‑apM1) and were co‑cultured with mature adipocytes stimulated with LPS...
October 26, 2017: Molecular Medicine Reports
keyword
keyword
35408
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"