keyword
MENU ▼
Read by QxMD icon Read
search

Hsp70 protein expression

keyword
https://www.readbyqxmd.com/read/29346421/the-absence-of-specific-yeast-heat-shock-proteins-leads-to-abnormal-aggregation-and-compromised-autophagic-clearance-of-mutant-huntingtin-proteins
#1
Ryan Higgins, Marie-Helene Kabbaj, Alexa Hatcher, Yanchang Wang
The functionality of a protein depends on its correct folding, but newly synthesized proteins are susceptible to aberrant folding and aggregation. Heat shock proteins (HSPs) function as molecular chaperones that aid in protein folding and the degradation of misfolded proteins. Trinucleotide (CAG) repeat expansion in the Huntingtin gene (HTT) results in the expression of misfolded Huntingtin protein (Htt), which contributes to the development of Huntington's disease. We previously found that the degradation of mutated Htt with polyQ expansion (Htt103QP) depends on both ubiquitin proteasome system and autophagy...
2018: PloS One
https://www.readbyqxmd.com/read/29345335/heat-shock-protein-70-modulates-neural-progenitor-cells-dynamics-in-human-neuroblastoma-sh-sy5y-cells-exposed-to-high-glucose-content
#2
Leila Salimi, Reza Rahbarghazi, Vahab Jafarian, Çıgır Biray Avci, Bakiye Goker Bagca, Neslihan Pinar Ozates, Majid Khaksar, Alireza Nourazarian
BACKGROUND: In the current experiment, detrimental effects of high glucose condition were investigated on human neuroblastoma cells. METHODS: Human neuroblastoma cell line SH-SY5Y were exposed to 5, 40 and 70 mM glucose over a period of 72 h. Survival rate and the proliferation of cells were analyzed by MTT and BrdU incorporation assays. Apoptosis was studied by the assays of flow cytometry and PCR array. In order to investigate the trans-differentiation capacity of the cell into mature neurons, we used immunofluorescence imaging to follow NeuN protein level...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29325453/age-related-lung-tissue-remodeling-due-to-the-local-distribution-of-mmp-2-timp-2-tgf-%C3%AE-and-hsp70
#3
Z Vitenberga, M Pilmane
Lung tissue remodeling requires complex interactions of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), transforming growth factor (TGF) family and heat shock protein 70 (Hsp70). We evaluated the appearance and distribution of MMP-2, TIMP-2, TGF-β1 and Hsp70 in lung tissue using immunohistochemistry. Stained structures were graded semiquantitatively. Overall, more MMP-2, TIMP-2, TGF-β1 and Hsp70 were observed in bronchial cartilage, bronchial and alveola repithelium, and among alveolar macrophages...
January 12, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29323151/downregulation-of-heat-shock-protein-70-impairs-osteogenic-and-chondrogenic-differentiation-in-human-mesenchymal-stem-cells
#4
Chenghai Li, Kristifor Sunderic, Steven B Nicoll, Sihong Wang
Human mesenchymal stem cells (hMSCs) show promise for bone and cartilage regeneration. Our previous studies demonstrated that hMSCs with periodic mild heating had enhanced osteogenic and chondrogenic differentiation with significantly upregulated heat shock protein 70 (HSP70). However, the role of HSP70 in adult tissue regeneration is not well studied. Here, we revealed an essential regulatory mechanism of HSP70 in osteogenesis and chondrogenesis using adult hMSCs stably transfected with specific shRNAs to knockdown HSP70...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29307045/a-comparison-of-cell-survival-and-heat-shock-protein-expression-after-radiation-in-normal-dermal-fibroblasts-microvascular-endothelial-cells-and-different-head-and-neck-squamous-carcinoma-cell-lines
#5
Dominique Muschter, Fabian Geyer, Richard Bauer, Tobias Ettl, Stephan Schreml, Frank Haubner
OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) shows increased radioresistance due to the manipulation of homeostatic mechanisms like the heat shock response. This study intended to comparatively analyze effects of ionizing radiation on different HNSCC cell lines (PCI) and normal human dermal fibroblasts (NHFs) and human dermal microvascular endothelial cells (HDMECs) to uncover differences in radiation coping strategies. MATERIALS AND METHODS: Proliferation (BrdU assay), apoptosis (caspase 3/7) and intracellular protein expression of heat shock protein (HSP)-70, and phosphorylated and total HSP27, determined by enzyme-linked immunosorbent assay (ELISA), were analyzed after exposure to increasing doses of ionizing radiation (2, 6, and 12 Gray, Gy)...
January 6, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29304094/positive-associations-between-upregulated-levels-of-stress-induced-phosphoprotein-1-and-matrix-metalloproteinase-9-in-endometriosis-adenomyosis
#6
Hsin-Shih Wang, Chia-Lung Tsai, Pi-Yueh Chang, Angel Chao, Ren-Chin Wu, Shun-Hua Chen, Chin-Jung Wang, Chih-Feng Yen, Yun-Shien Lee, Tzu-Hao Wang
Stress-induced phosphoprotein-1 (STIP1), an adaptor protein that coordinates the functions of HSP70 and HSP90 in protein folding, has been implicated in the development of human gynecologic malignancies. This case-control study investigates STIP1 serum levels and tissue expression in relation to endometriosis/adenomyosis in Taiwanese population. Female patients with surgically confirmed endometriosis/adenomyosis were compared with women free of endometriosis/adenomyosis. Serum STIP1 levels were measured using an enzyme-linked immunosorbent assay and surgical tissues were analyzed by immunohistochemistry...
2018: PloS One
https://www.readbyqxmd.com/read/29303960/lhcsr-expression-under-hsp70-rbcs2-promoter-as-a-strategy-to-increase-productivity-in-microalgae
#7
Federico Perozeni, Giulio Rocco Stella, Matteo Ballottari
Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high thermal dissipation of the light absorbed by the outer layers of the cultures exposed to high light caused by the activation of a photoprotective mechanism called non-photochemical quenching (NPQ). In the model organism for green algae Chlamydomonas reinhardtii, NPQ is triggered by pigment binding proteins called light-harvesting-complexes-stress-related (LHCSRs), which are over-accumulated in high light...
January 5, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29301687/heat-shock-response-and-shape-regulation-during-newt-tail-regeneration
#8
Elena Radugina, Eleonora Grigoryan
Regenerating newt tail has recently been found to react to hypergravity in a stable and reproducible way - by curving downwards. Such morphogenetic effect of non-specific physical factor applied to a complex structure of an adult animal is a rare phenomenon with unknown molecular basis. For the first steps of unraveling this basis we've chosen heat shock proteins (HSPs) as promising candidates. Morphometrical analysis of tail regeneration was performed in aquarium (control), on substrate (relative hypergravity) and in aquarium under weekly application of heat shock...
January 2018: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29301599/experimental-study-on-the-protection-of-agrimony-extracts-from-different-extracting-methods-against-cerebral-ischemia-reperfusion-injury
#9
Hui-Yuan Zhu, Yu-Long Bie, Jiang Wang, Jing Gao, Bing-Yue Yang, Hai-Tong Wan
Objective To study the protective effect of agrimony extracts from different extracting methods on cerebral ischemia-reperfusion injury in rats, in order to optimize the extraction scheme of agrimony. Methods Male rats were randomly assigned into seven groups: 1. Sham-operated group, 2. Untreated MCAO group (MCAO), 3. Petroleum ether extract of Agrimonia pilosa treated MCAO group (PEA), 4. Ethyl acetate extract of Agrimonia pilosa treated MCAO group (EAEA), 5. Ethanol extract of Agrimonia pilosa treated MCAO group (EEA), 6...
December 30, 2017: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/29300467/disrupted-hydrogen-bond-network-and-impaired-atpase-activity-in-an-hsc70-cysteine-mutant
#10
John P O'Donnell, Heather M Marsh, Holger Sondermann, Carolyn Sevier
The ATPase domain of the 70-kD heat shock protein (Hsp70) family members shows a high degree of sequence, structural, and functional homology across species. A broadly conserved residue within the Hsp70 ATPase domain that captured our attention is an unpaired cysteine, positioned proximal to the site of nucleotide binding. Prior studies of several Hsp70 family members show this cysteine is not required for Hsp70 ATPase activity, yet select amino acid replacements of the cysteine can dramatically alter ATP hydrolysis...
January 4, 2018: Biochemistry
https://www.readbyqxmd.com/read/29298131/a-systematic-review-of-p53-regulation-of-oxidative-stress-in-skeletal-muscle
#11
Kaitlyn Beyfuss, David A Hood
BACKGROUND: p53 is a tumor suppressor protein involved in regulating a wide array of signaling pathways. The role of p53 in the cell is determined by the type of imposed oxidative stress, its intensity and duration. The last decade of research has unravelled a dual nature in the function of p53 in mediating the oxidative stress burden. However, this is dependent on the specific properties of the applied stress and thus requires further analysis. METHODS: A systematic review was performed following an electronic search of Pubmed, Google Scholar, and ScienceDirect databases...
January 3, 2018: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/29296843/the-severe-phenotype-of-diamond-blackfan-anemia-is-modulated-by-heat-shock-protein-70
#12
Marc Gastou, Sarah Rio, Michaël Dussiot, Narjesse Karboul, Hélène Moniz, Thierry Leblanc, Margaux Sevin, Patrick Gonin, Jérome Larghéro, Carmen Garrido, Anupama Narla, Narla Mohandas, William Vainchenker, Olivier Hermine, Eric Solary, Lydie Da Costa
Diamond-Blackfan anemia (DBA) is a rare congenital bone marrow failure syndrome that exhibits an erythroid-specific phenotype. In at least 70% of cases, DBA is related to a haploinsufficient germ line mutation in a ribosomal protein (RP) gene. Additional cases have been associated with mutations in GATA1. We have previously established that the RPL11+/Mut phenotype is more severe than RPS19+/Mut phenotype because of delayed erythroid differentiation and increased apoptosis of RPL11+/Mut erythroid progenitors...
October 10, 2017: Blood Advances
https://www.readbyqxmd.com/read/29290964/tetratricopeptide-repeat-domain-3-overexpression-tends-to-form-aggregates-and-inhibit-ubiquitination-and-degradation-of-dna-polymerase-%C3%AE
#13
Yueqing Gong, Xiaolan Wang, Xuan Shang, Sheng Ping Xiao, Wanjie Li, Yu Shang, Fei Dou
Tetratricopeptide repeat (TPR) domain 3 (TTC3) is a protein that contains canonical RING finger and TPR motifs. It is encoded by the TTC3 gene located in the Down syndrome critical region (DSCR). In this study, we used a yeast two-hybrid assay to identify several proteins that physically interact with TTC3, including heat shock proteins and DNA polymerase γ (POLG). When TTC3 was overexpressed in mammalian cells, the ubiquitination of POLG was inhibited and its degradation slowed. High TTC3 protein expression led to the development of intracellular TTC3 aggregates, which also contained POLG...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29289712/proteome-changes-of-beef-in-nellore-cattle-with-different-genotypes-for-tenderness
#14
Alessandra F Rosa, Cristina T Moncau, Mirele D Poleti, Leydiana D Fonseca, Julio C C Balieiro, Saulo L E Silva, Joanir P Eler
In the present study, 155 Nellore cattle were genotyped for the CAPN4751 and UOGCAST polymorphisms and phenotyped for shear force (SF) at 1, 7 and 14days aging. The effects of different genotypic combinations were evaluated on the Longissimus muscle proteomic profile using 2DE and mass spectrometry. A significant association was found between genotypes for UOGCAST and CAPN4751 and meat tenderness. The CC genotype for both markers was favorable for lesser shear force than TT. A total of 40 spots showed significant differential expression profiles (P<0...
December 13, 2017: Meat Science
https://www.readbyqxmd.com/read/29288301/arsenic-and-or-copper-caused-inflammatory-response-via-activation-of-inducible-nitric-oxide-synthase-pathway-and-triggered-heat-shock-protein-responses-in-testis-tissues-of-chicken
#15
Yizhi Shao, Hongjing Zhao, Yu Wang, Juanjuan Liu, Jinglun Li, Hongliang Chai, Mingwei Xing
The aim of this study is to investigate the effects of arsenic (As) and copper (Cu) on the inflammatory response, and the protective roles of heat shock proteins (Hsps) in chicken testes. Seventy-two 1-day-old male Hy-line chickens were treated with 30 mg/kg feed of arsenic trioxide (As2O3) and/or 300 mg/kg feed of copper sulfate (CuSO4) for 4, 8, and 12 weeks. The histological changes, inducible nitric oxide synthase (iNOS) activity, and the expressions of Hsps and inflammatory cytokines were detected. The results showed that slight histology changes were obvious in the testis tissue exposure to treatment groups...
December 29, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29285257/sensitization-of-lung-cancer-cells-by-altered-dimerization-of-hsp27
#16
Byeol Choi, Seul-Ki Choi, You Na Park, Soo-Yeon Kwak, Hwa Jeong Lee, Youngjoo Kwon, Younghwa Na, Yun-Sil Lee
Heat shock protein 27 (HSP27, HSPB1) induces resistance to anticancer drugs in various cancer types, including non-small cell lung cancer (NSCLC). Therefore, pharmacological inhibition of HSP27 in NSCLC may be a good strategy for anticancer therapy. Unlike other HSPs such as HSP90 and HSP70, small molecule approaches for neutralization of HSP27 are not well established because of the absence of an ATP binding domain. Previously, small molecules with altered cross linking activity of HSP27, were identified to inhibit building a large oligomer led to sensitization in combination with radiation and chemotherapeutic drugs...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29285052/suppression-of-alzheimer-s-disease-related-phenotypes-by-the-heat-shock-protein-70-inducer-geranylgeranylacetone-in-app-ps1-transgenic-mice-via-the-erk-p38-mapk-signaling-pathway
#17
Yuan Sun, Jiang-Rong Zhang, Shuyan Chen
HSP70 overexpression has a remedying effect in neurodegenerative diseases. In Alzheimer's disease (AD), the suppressive effects of HSP70 overexpression on AD-related phenotypes and the underlying mechanisms are unknown. In the current study, the effect of geranylgeranylacetone (GGA), a non-toxic inducer of heat shock protein (HSP)-70 expression, on cognitive function and other pathological phenotypes were evaluated in APP/PS1 mice. It was observed that all doses of orally administered GGA (200, 400, and 800 mg/kg/day) improved cognitive deficit (P<0...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29284147/effects-of-dietary-supplementation-of-four-strains-of-lactic-acid-bacteria-on-growth-immune-related-response-and-genes-expression-of-the-juvenile-sea-cucumber-apostichopus-japonicus-selenka
#18
Chao Li, Yichao Ren, Senhao Jiang, Shun Zhou, Jinshan Zhao, Renjie Wang, Yongmei Li
A feeding experiment was conducted to evaluate the effects of four strains of lactic acid bacteria (LAB) [i.e. Lactobacillus plantarum LL11 (LP), Weissella confuse LS13 (WC), Lactococcus lactis LH8 (LL) and Enterococcus faecalis LC3 (ES)] isolated from marine fish on growth, immune response and expression levels of immune-related gens in body wall of juvenile sea cucumber Apostichopus japonicus. As a result, sea cucumber had better growth performance fed supplementation of LP and ES than the control group (P < ...
December 25, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29281627/an-essential-regulatory-function-of-the-dnak-chaperone-dictates-the-decision-between-proliferation-and-maintenance-in-caulobacter-crescentus
#19
Frederic D Schramm, Kristina Heinrich, Marietta Thüring, Jörg Bernhardt, Kristina Jonas
Hsp70 chaperones are well known for their important functions in maintaining protein homeostasis during thermal stress conditions. In many bacteria the Hsp70 homolog DnaK is also required for growth in the absence of stress. The molecular reasons underlying Hsp70 essentiality remain in most cases unclear. Here, we demonstrate that DnaK is essential in the α-proteobacterium Caulobacter crescentus due to its regulatory function in gene expression. Using a suppressor screen we identified mutations that allow growth in the absence of DnaK...
December 27, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29277604/tolerance-to-hypometabolism-and-arousal-induced-by-hibernation-in-the-apple-snail-pomacea-canaliculata-caenogastropoda-ampullariidae
#20
Maximiliano Giraud-Billoud, Alfredo Castro-Vazquez, Alejandra D Campoy-Diaz, Pablo M Giuffrida, Israel A Vega
Pomacea canaliculata may serve as a model organism for comparative studies of oxidative damage and antioxidant defenses in active, hibernating and arousing snails. Oxidative damage (as TBARS), free radical scavenging capacity (as ABTS+ oxidation), uric acid (UA) and glutathione (GSH) concentrations, activities of superoxide dismutase (SOD) and catalase (CAT), and the protein expression levels of heat shock proteins (Hsp70, Hsc70, Hsp90) were studied in digestive gland, kidney and foot. Tissue TBARS of hibernating snails (45days) was higher than active snails...
December 19, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
keyword
keyword
35407
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"