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Hsp70 protein expression

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https://www.readbyqxmd.com/read/27908666/molecular-cloning-and-expression-analysis-of-five-heat-shock-protein-70-hsp70-family-members-in-lateolabrax-maculatus-with-vibrio-harveyi-infection
#1
Ying-Li Han, Cong-Cong Hou, Chen Du, Jun-Quan Zhu
Heat shock proteins 70 (HSP70s) are molecular chaperones that aid in protection against environmental stress. In this study, we cloned and characterized five members of the HSP70 family (designated as HSPa1a, HSC70-1, HSC70-2, HSPa4 and HSPa14) from Lateolabrax maculatus using rapid amplification cDNA ends (RACE). Multiple sequence alignment and structural analysis revealed that all members of the HSP70 family had a conserved domain architecture, with some distinguishing features unique to each HSP70. Quantitative real-time (qPCR) analysis revealed that all members of the HSP70 family were ubiquitously and differentially expressed in all major types of tissues, including testicular tissue...
November 28, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27906059/a-mitochondrial-hsp70-hspa9b-is-linked-to-miltefosine-resistance-and-stress-response-in-leishmania-donovani
#2
P Vacchina, B Norris-Mullins, E S Carlson, M A Morales
BACKGROUND: Protozoan parasites of the genus Leishmania are responsible for leishmaniasis, a neglected tropical disease affecting millions worldwide. Visceral leishmaniasis (VL), caused by Leishmania donovani, is the most severe form of leishmaniasis with high rates of mortality if left untreated. Current treatments include pentavalent antimonials and amphotericin B. However, high toxicity and emergence of resistance hinder the success of these options. Miltefosine (HePC) is the first oral treatment available for leishmaniasis...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27903966/pde5-inhibitors-enhance-the-lethality-of-pemetrexed-through-inhibition-of-multiple-chaperone-proteins-and-via-the-actions-of-cyclic-gmp-and-nitric-oxide
#3
Laurence Booth, Jane L Roberts, Andrew Poklepovic, Sarah Gordon, Paul Dent
Phosphodiesterase 5 (PDE5) inhibitors prevent the breakdown of cGMP that results in prolonged protein kinase G activation and the generation of nitric oxide. PDE5 inhibitors enhanced the anti-NSCLC cell effects of the NSCLC therapeutic pemetrexed. [Pemetrexed + sildenafil] activated an eIF2α - ATF4 - CHOP - Beclin1 pathway causing formation of toxic autophagosomes; activated a protective IRE1 - XBP-1 - chaperone induction pathway; and activated a toxic eIF2α - CHOP - DR4 / DR5 / CD95 induction pathway. [Pemetrexed + sildenafil] reduced the expression of c-FLIP-s, MCL-1 and BCL-XL that was blocked in a cell-type -dependent fashion by either over-expression of HSP90 / GRP78 / HSP70 / HSP27 or by blockade of eIF2α-CHOP signaling...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27893783/role-of-pten-in-oxidative-stress-and-dna-damage-in-the-liver-of-whole-body-pten-haplodeficient-mice
#4
Ezgi Eyluel Bankoglu, Oliver Tschopp, Johannes Schmitt, Philipp Burkard, Daniel Jahn, Andreas Geier, Helga Stopper
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in early T2DM and excessive insulin can cause elevated reactive oxygen species (ROS) production and genomic instability. ROS are important for various cellular functions in signaling and host defense. However, elevated ROS formation is thought to be involved in cancer induction...
2016: PloS One
https://www.readbyqxmd.com/read/27890585/cadmium-in%C3%A2-vivo-exposure-alters-stress-response-and-endocrine-related-genes-in-the-freshwater-snail-physa-acuta-new-biomarker-genes-in-a-new-model-organism
#5
Pedro Martínez-Paz, Mónica Morales, Paloma Sánchez-Argüello, Gloria Morcillo, José Luis Martínez-Guitarte
The freshwater snail Physa acuta is a sensitive organism to xenobiotics that is appropriate for toxicity testing. Cadmium (Cd) is a heavy metal with known toxic effects on several organisms, which include endocrine disruption and activation of the cellular stress responses. There is scarce genomic information on P. acuta; hence, in this work, we identify several genes related to the hormonal system, the stress response and the detoxification system to evaluate the effects of Cd. The transcriptional activity of the endocrine-related genes oestrogen receptor (ER), oestrogen-related receptor (ERR), and retinoid X receptor (RXR), the heat shock proteins genes hsp70 and hsp90 and a metallothionein (MT) gene was analysed in P...
November 24, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27890044/effect-of-dietary-manganese-on-antioxidant-status-and-expressions-of-heat-shock-proteins-and-factors-in-tissues-of-laying-broiler-breeders-under-normal-and-high-environmental-temperatures
#6
Yong-Wen Zhu, Lin Lu, Wen-Xiang Li, Li-Yang Zhang, Cheng Ji, Xi Lin, Hsiao-Ching Liu, Jack Odle, Xu-Gang Luo
To investigate the effect of Mn on antioxidant status and on the expressions of heat shock proteins/factors in tissues of laying broiler breeders subjected to heat challenge, we used a completely randomised design (n 6) with a factorial arrangement of 2 environmental temperatures (normal, 21±1°C, and high, 32±1°C)×3 dietary Mn treatments (a Mn-unsupplemented basal diet (CON), or a basal diet supplemented with 120 mg Mn/kg diet, either as inorganic Mn sulphate (iMn) or as organic Mn proteinate (oMn)). There were no interactions (P>0·10) between environmental temperature and dietary Mn in any of the measured indices...
November 28, 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/27888131/hsp70-and-hsp90-are-involved-in-shrimp-penaeus-vannamei-tolerance-to-ahpnd-causing-strain-of-vibrio-parahaemolyticus-after-non-lethal-heat-shock
#7
Wisarut Junprung, Premruethai Supungul, Anchalee Tassanakajon
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in farmed penaeid shrimp throughout Asia. Previous studies reported that a non-lethal heat shock (NLHS) could enhance disease tolerance in aquatic animals. Here, we investigate whether the NLHS could enhance the survival of shrimp Peneaus vannamei upon challenge with an AHPND-causing strain of V. Parahaemolyticus (VPAHPND). Two NLHS conditions, acute and chronic NLHSs, were used...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27879259/unexpected-role-for-p19ink4d-in-post-transcriptional-regulation-of-gata1-and-modulation-of-human-terminal-erythropoiesis
#8
Xu Han, Jieying Zhang, Yuanliang Peng, Minyuan Peng, Xiao Chen, Huiyong Chen, Jianhui Song, Xiao Hu, Mao Ye, Jianglin Li, Vijay G Sankaran, Christopher D Hillyer, Narla Mohandas, Xiuli An, Jing Liu
Terminal erythroid differentiation is tightly coordinated with cell cycle exit, which is regulated by cyclins, cyclin-dependent kinases and cyclin-dependent kinase inhibitors (CDKI), yet their roles in erythropoiesis remain to be fully defined. We show here that p19(INK4d), a member of CDKI family, is abundantly expressed in erythroblasts and that p19(INK4d) knockdown delayed erythroid differentiation, inhibited cell growth, led to increased apoptosis and generation of abnormally nucleated late stage erythroblasts...
November 22, 2016: Blood
https://www.readbyqxmd.com/read/27872596/different-relationship-between-hsp70-mrna-and-hsp70-levels-in-the-heat-shock-response-of-two-salmonids-with-dissimilar-temperature-preference
#9
Mario Lewis, Miriam Götting, Katja Anttila, Mirella Kanerva, Jenni M Prokkola, Eila Seppänen, Irma Kolari, Mikko Nikinmaa
The heat shock response (HSR) refers to the rapid production of heat shock proteins (hsps) in response to a sudden increase in temperature. Its regulation by heat shock factors is a good example of how gene expression is transcriptionally regulated by environmental stresses. In contrast, little is known about post-transcriptional regulation of the response. The heat shock response is often used to characterize the temperature tolerance of species with the rationale that whenever the response sets on, a species is approaching its lethal temperature...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27872197/plasma-exosomes-are-enriched-in-hsp70-and-modulated-by-stress-and-cortisol-in-rainbow-trout
#10
Erin Faught, Lynsi Henrickson, Mathilakath M Vijayan
Exosomes are endosomally-derived vesicles that are secreted from cells and contain a suite of molecules, including proteins and nucleic acids. Recent studies suggest the possibility that exosomes in circulation may be affecting recipient target cell function, but the modes of action are unclear. Here we tested the hypothesis that exosomes are in circulation in fish plasma and that these vesicles are enriched with heat shock protein 70 (Hsp70). Exosomes were isolated from rainbow trout (Oncorhynchus mykiss) plasma using differential centrifugation and their presence confirmed by transmission electron microscopy and the exosomal marker acetylcholinesterase...
November 21, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27864821/effect-of-alterations-in-apoptotic-pathway-on-development-of-metabolic-syndrome-in-patients-with-psoriasis-vulgaris
#11
S Korkmaz, H Korkmaz
OBJECTIVES: The purpose of this study is to evaluate the role of changes in gene expression of apoptosis activators (BAX, cytocrome-c [cyt-c], and caspase-3) and apoptosis inhibitors (BCL2, survivin, cyclin D1 [CCND1], SOD, catalase 3 [CAT], glutathione synthetase [GS], heat shock protein [HSP]27, HSP60, HSP70, and HSP90) on the development of metabolic syndrome (MetS) in patients with psoriasis vulgarins. METHODS: Fifty patients with psoriasis were enrolled in the study, 25 of whom had MetS...
November 18, 2016: British Journal of Dermatology
https://www.readbyqxmd.com/read/27862401/identification-and-changes-in-the-seasonal-concentrations-of-heat-shock-proteins-in-roe-deer-capreolus-capreolus-epididymides
#12
A M Majewska, W Kordan, M Koziorowska-Gilun, P Wysocki
Heat shock proteins (HSPs) act as molecular chaperones with important regulatory functions. HSPs are considered to be essential factors in animal reproduction. In view of seasonal variations in the secretory activity of the reproductive tract of mature roe deer (Capreolus capreolus), the aims of this study were to identify HSPs in the epididymides and compare the expression of the identified proteins in three periods of the reproductive season. Two-dimensional polyacrylamide gel electrophoresis revealed the highest number of polypeptides in homogenates of epididymal tissues and in caput, corpus and cauda epididymal fluids throughout the reproductive season...
November 13, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27861646/effect-of-geranylgeranylacetone-on-the-protection-of-retinal-ganglion-cells-in-a-mouse-model-of-normal-tension-glaucoma
#13
Zhenyu Dong, Yasuhiro Shinmei, Yoko Dong, Saori Inafuku, Junichi Fukuhara, Ryo Ando, Nobuyoshi Kitaichi, Atsuhiro Kanda, Kohichi Tanaka, Kousuke Noda, Takayuki Harada, Shinki Chin, Susumu Ishida
Glaucoma is characterized by axonal degeneration of retinal ganglion cells (RGCs) and apoptotic death of their cell bodies, and lowering intraocular pressure is associated with an attenuation of progressive optic nerve damage. Nevertheless, intraocular pressure (IOP) reduction alone was not enough to inhibit the progression of disease, which suggests the contribution of other factors to the glaucoma pathogenesis. In this study, we investigated the cytoprotective effect of geranylgeranylacetone (GGA) on RGCs degeneration using a normal tension glaucoma (NTG) mouse model, which lacks glutamate/aspartate transporter (GLAST) and demonstrates spontaneous RGC and optic nerve degeneration without elevated intraocular pressure (IOP)...
October 2016: Heliyon
https://www.readbyqxmd.com/read/27852853/coat-protein-regulation-by-ck2-cpip-hsp70-and-chip-is-required-for-potato-virus-a-replication-and-coat-protein-accumulation
#14
Andres Lõhmus, Anders Hafrén, Kristiina Mäkinen
: We demonstrate here that coat protein (CP) phosphorylation by protein kinase CK2 and a chaperone system formed by two heat-shock proteins, CP-interacting protein (CPIP) and HSP70, are both essential for Potato virus A (PVA; genus Potyvirus) replication and that all of these host proteins have the capacity to contribute to the level of PVA CP accumulation. An E3 ubiquitin ligase called carboxyl terminus Hsc70-interacting protein (CHIP), which may participate in the CPIP-HSP70-mediated CP degradation, is also needed for robust PVA gene expression...
November 16, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27847173/enhanced-protective-efficacy-against-tuberculosis-provided-by-a-recombinant-urease-deficient-bcg-expressing-heat-shock-protein-70-major-membrane-protein-ii-having-pest-sequence
#15
Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Tetsu Mukai, Satoshi Mitarai, Saburo Yamamoto, Masahiko Makino
Enhancement of the T cell-stimulating ability of Mycobacterium bovis BCG (BCG) is necessary to develop an effective tuberculosis vaccine. For this purpose, we introduced the PEST-HSP70-major membrane protein-II (MMPII)-PEST fusion gene into ureC-gene depleted recombinant (r) BCG to produce BCG-PEST. The PEST sequence is involved in the proteasomal processing of antigens. BCG-PEST secreted the PEST-HSP70-MMPII-PEST fusion protein and more efficiently activated human monocyte-derived dendritic cells (DCs) in terms of phenotypic changes and cytokine productions than an empty-vector-introduced BCG or HSP70-MMPII gene-introduced ureC gene-depleted BCG (BCG-DHTM)...
December 7, 2016: Vaccine
https://www.readbyqxmd.com/read/27846716/-mechanisms-of-protein-homeostasis-regulation-in-cancer-development
#16
F Trčka, P Müller, B Vojtěšek
BACKGROUND: The proteome of eukaryotic cells represents a complex system. Its components are exposed to various intrinsic and extrinsic stresses. Therefore, the function of the cellular proteome is dependent on the existence of compensatory mechanisms balancing the inner protein homeostasis - proteostasis. These mechanisms involve the network of molecular chaperones and transcriptional program regulating their expression. The process of cancerogenesis is accompanied by significant changes in the intracellular milieu of cancer cells - temperature, pH, availability of nutrients...
2016: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
https://www.readbyqxmd.com/read/27845306/effects-of-aspartame-on-hsp70-bcl-2-and-bax-expression-in-immune-organs-of-wistar-albino-rats
#17
Arbind Kumar Choudhary, Rathinasamy Sheela Devi
Aspartame, a "first generation sweetener", is widely used in a variety of foods, beverages, and medicine. The FDA has determined the acceptable daily intake (ADI) value of aspartame to be 50 mg/kg·day, while the JECFA (Joint FAO/WHO Expert Committee on Food Additives) has set this value at 40 mg/kg of body weight/day. Safety issues have been raised about aspartame due to its metabolites, specifically toxicity from methanol and/or its systemic metabolites formaldehyde and formic acid. The immune system is now recognized as a target organ for many xenobiotics, such as drugs and chemicals, which are able to trigger unwanted apoptosis or to alter the regulation of apoptosis...
September 2016: Journal of Biomedical Research
https://www.readbyqxmd.com/read/27842496/proteomics-analysis-reveals-novel-host-molecular-mechanisms-associated-with-thermotherapy-of-ca-liberibacter-asiaticus-infected-citrus-plants
#18
Chika C Nwugo, Melissa S Doud, Yong-Ping Duan, Hong Lin
BACKGROUND: Citrus Huanglongbing (HLB), which is linked to the bacterial pathogen 'Ca. Liberibacter asiaticus' (Las), is the most devastating disease of citrus plants, and longer-term control measures via breeding or genetic engineering have been unwieldy because all cultivated citrus species are susceptible to the disease. However, the degree of susceptibility varies among citrus species, which has prompted efforts to identify potential Las resistance/tolerance-related genes in citrus plants for application in breeding or genetic engineering programs...
November 14, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27836720/effect-of-dietary-poly-%C3%AE-hydroxybutyrate-phb-on-growth-performance-intestinal-health-status-and-body-composition-of-pacific-white-shrimp-litopenaeus-vannamei-boone-1931
#19
Yafei Duan, Yue Zhang, Hongbiao Dong, Xiaoting Zheng, Yun Wang, Hua Li, Qingsong Liu, Jiasong Zhang
In the present study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on the growth performance, intestinal digestive and immune function, intestinal short-chain fatty acids (SCFA) content and body composition of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) was evaluated. The shrimp was fed for 35 days with four different diets: 0%, 1%, 3% and 5% PHB supplemented feed. The results indicated that supplementation of PHB significantly increased the growth performance of the shrimp, and the feed conversion rate (FCR) in 3%PHB treatment group was significantly lower than the control (P < 0...
November 8, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27831465/dynamic-control-of-hsf1-during-heat-shock-by-a-chaperone-switch-and-phosphorylation
#20
Xu Zheng, Joanna Krakowiak, Nikit Patel, Ali Beyzavi, Jideofor Ezike, Ahmad S Khalil, David Pincus
Heat shock factor (Hsf1) regulates the expression of molecular chaperones to maintain protein homeostasis. Despite its central role in stress resistance, disease and aging, the mechanisms that control Hsf1 activity remain unresolved. Here we show that in budding yeast, Hsf1 basally associates with the chaperone Hsp70 and this association is transiently disrupted by heat shock, providing the first evidence that a chaperone repressor directly regulates Hsf1 activity. We develop and experimentally validate a mathematical model of Hsf1 activation by heat shock in which unfolded proteins compete with Hsf1 for binding to Hsp70...
November 10, 2016: ELife
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