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Hsp70 protein expression

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https://www.readbyqxmd.com/read/28731175/heat-shock-induced-hikeshi-protects-cell-viability-via-nuclear-translocation-of-heat-shock-protein-70
#1
Toru Yanoma, Kyoichi Ogata, Takehiko Yokobori, Munenori Ide, Erito Mochiki, Yoshitaka Toyomasu, Mitsuhiro Yanai, Norimichi Kogure, Akiharu Kimura, Masaki Suzuki, Nobuhiro Nakazawa, Tuya Bai, Tetsunari Oyama, Takayuki Asao, Ken Shirabe, Hiroyuki Kuwano
Heat shock proteins (HSPs), particularly HSP70, help restore normal cellular function following damage caused by stressors. HSP expression in tumor tissues indicates cancer progression, and while the development of HSP inhibitors is progressing, these substances are not widely used to treat cancer. HIKESHI (C11orf73) does not control the intracellular movement of HSP70 at normal temperatures; however, it does regulate the function and movements of HSP70 during heat shock. In this study, we examined the intracellular movement of HSP70 during heat shock to investigate the significance of HIKESHI expression in gastric cancer (GC) and determine if HIKESHI inhibition has cytotoxic effects...
July 21, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28730731/induction-of-stress-and-immune-associated-genes-in-the-indian-meal-moth-plodia-interpunctella-against-envenomation-by-the-ectoparasitoid-bracon-hebetor
#2
Tahir Shafeeq, Zain UlAbdin, Kyeong-Yeoll Lee
Envenomation is an important process in parasitism by parasitic wasps; it suppresses the immune and development of host insects. However, the molecular mechanisms of host responses to envenomation are not yet clear. This study aimed to determine the transcription-level responses of the Indian meal moth Plodia interpunctella against envenomation of the ectoparasitoid Bracon hebetor. Quantitative real-time reverse-transcription PCR was used to determine the transcriptional changes of 13 selected genes, which are associated with development, metabolism, stress, or immunity, in the feeding and wandering fifth instar larvae over a 4-day period after envenomation...
July 21, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28730576/effects-of-boron-supplementation-on-expression-of-hsp70-in-the-spleen-of-african-ostrich
#3
Khaliq Haseeb, Jing Wang, Ke Xiao, Ke-Li Yang, Peng-Peng Sun, Xing-Tong Wu, You Luo, Hui Song, Hua-Zhen Liu, Ju-Ming Zhong, Ke-Mei Peng
Increased synthesis of heat shock protein 70 (Hsp70) occurs in prokaryotes and eukaryotes in response to physiological, environmental, and chemical exposures, thus allowing the cell survival from fatal conditions. Hsp70 cytoprotective properties may be clarified by its anti-apoptotic function. Boron has been reported to play an essential role in various organ developments and metabolisms. However, it is not known if boron is also able to modulate the Hsp70. In the present study, the actions of boron on ostrich spleen and expression level of Hsp70 were investigated...
July 20, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28730074/protection-of-retinal-ganglion-cells-against-optic-nerve-injury-by-induction-of-ischemic-preconditioning
#4
Xia Liu, Jiu-Ping Liang, Ou Sha, Song-Juan Wang, Heng-Guo Li, Eric Y P Cho
AIM: To explore if ischemic preconditioning (IPC) can enhance the survival of retinal ganglion cells (RGCs) after optic nerve axotomy. METHODS: Twenty-four hours prior to retinal ischemia 60min or axotomy, IPC was applied for ten minutes in groups of (n=72) animals. The survival of RGCs, the cellular expression of heat shock protein 27 (HSP27) and heat shock protein 70 (HSP70) and the numbers of retinal microglia in the different groups were quantified at 7 and 14d post-injury...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28725954/proteasome-stress-triggers-death-of-sh-sy5y-and-t98g-cells-via-different-cellular-mechanisms
#5
Ivana Pilchova, Katarina Klacanova, Katarina Dibdiakova, Simona Saksonova, Andrea Stefanikova, Eva Vidomanova, Lucia Lichardusova, Jozef Hatok, Peter Racay
Overload or dysfunction of ubiquitin-proteasome system (UPS) is implicated in mechanisms of neurodegeneration associated with neurodegenerative diseases, e.g. Parkinson and Alzheimer disease, and ischemia-reperfusion injury. The aim of this study was to investigate the possible association between viability of neuroblastoma SH-SY5Y and glioblastoma T98G cells treated with bortezomib, inhibitor of 26S proteasome, and accumulation of ubiquitin-conjugated proteins with respect to direct cytotoxicity of aggregates of ubiquitin-conjugated proteins...
July 19, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28717326/honey-can-repairing-damage-of-liver-tissue-due-to-protein-energy-malnutrition-through-induction-of-endogenous-stem-cells
#6
R Heru Prasetyo, Eka Pramyrtha Hestianah
AIM: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells. MATERIALS AND METHODS: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715410/cloning-of-hsp90-expression-and-localization-of-hsp70-90-in-different-tissues-including-lactating-non-lactating-yak-bos-grunniens-breast-tissue
#7
Penggang Liu, Sijiu Yu, Yan Cui, Junfeng He, Chuan Yu, Zexing Wen, Yangyang Pan, Kun Yang, Liangli Song, Xue Yang
The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting (WB), immunohistochemistry and immunofluorescence assays were utilized to analyze the expressions of HSP70/90 mRNA and protein in different tissues. Sequence analysis showed that HSP90 is a conserved molecular chaperone of female yaks...
2017: PloS One
https://www.readbyqxmd.com/read/28714602/genome-wide%C3%A2-identification-and-characterization%C3%A2-of%C3%A2-hsp-gene-superfamily-in-whitefly-bemisia-tabaci-and-expression-profiling%C3%A2-analysis%C3%A2-under-temperature-stress
#8
Xin-Ru Wang, Chao Wang, Fei-Xue Ban, Dan-Tong Zhu, Shu-Sheng Liu, Xiao-Wei Wang
Heat shock proteins (Hsp) are essential molecular chaperones that play important roles in the stress stimulation of insects. Bemisia tabaci, a phloem feeder and invasive species, can cause extensive crop damage through direct feeding and transmission of plant viruses. Here we employed comprehensive genomics approaches to identity HSP superfamily members in Middle East Asia Minor 1 whitefly genome. In total, we identified 26 Hsp genes, including 3 Hsp90, 17 Hsp70, 1 Hsp60 and 5 sHSP (small heat shock protein, sHSP) genes...
July 17, 2017: Insect Science
https://www.readbyqxmd.com/read/28714586/activation-of-autophagy-is-involved-in-the-protective-effect-of-17%C3%AE-oestradiol-on-endotoxaemia-induced-multiple-organ-dysfunction-in-ovariectomized-rats
#9
Ming-Tzeung Chung, Yen-Mei Lee, Hsin-Hsueh Shen, Pao-Yun Cheng, Yu-Chen Huang, Yu-Ju Lin, Yu-Yang Huang, Kwok-Keung Lam
Oestrogens have been reported to attenuate acute inflammation in sepsis. In this study, the effects of long-term oestrogen replacement with 17β-oestradiol (E2 ) on endotoxaemia-induced circulatory dysfunction and multiple organ dysfunction syndrome were evaluated in ovariectomized (Ovx) rats. E2 (50 μg/kg, s.c., 3 times/week) was administered for 8 weeks, followed by the induction of endotoxaemia by intravenous infusion of lipopolysaccharides (LPS; 30 mg/kg/4 hrs). Oestrogen deficiency induced by ovariectomy for 9 weeks augmented the LPS-induced damage, including endotoxic shock, myocardial contractile dysfunction, renal dysfunction and rhabdomyolysis...
July 17, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28711961/heat-shock-protein-70-nitric-oxide-effect-on-stretched-tubular-epithelial-cells-linked-to-wt-1-cytoprotection-during-neonatal-obstructive-nephropathy
#10
Luciana Mazzei, Fernando Darío Cuello-Carrión, Neil Docherty, Walter Manucha
BACKGROUND: Mechanical stress is a key pathogenic driver of apoptosis in the tubular epithelium in obstructive nephropathy. Heat shock protein 70 (Hsp70) and Wilms' tumor (WT-1) have been proposed to represent linked downstream effectors of the cytoprotective properties of NO. In the present study, we sought to evaluate whether the cytoprotective effects of L-arginine in neonatal obstructive nephropathy may be associated with NO-dependent increases in WT-1 and Hsp70 expression. METHODS: Neonatal Wistar-Kyoto rats were submitted to complete unilateral ureteral obstruction (UUO) and treated thereafter with vehicle, L-NAME or L-arginine by daily gavage for 14 days to block or augment NO levels, respectively...
July 15, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28711525/novel-hsp90-inhibitor-platycodin-d-disrupts-hsp90-cdc37-complex-and-enhances-the-anticancer-effect-of-mtor-inhibitor
#11
Ting Li, Xin Chen, Xiao-Yang Dai, Bin Wei, Qin-Jie Weng, Xiuping Chen, De-Fang Ouyang, Ru Yan, Zhang-Jian Huang, Hu-Lin Jiang, Hong Zhu, Jin-Jian Lu
Heat shock protein 90 (Hsp90) is a critically conserved molecular chaperone protein and promising therapeutic target for cancer treatment. In this study, platycodin D (PD), a saponin isolated from traditional Chinese herb Platycodonis Radix, was identified as a novel Hsp90 inhibitor. We verified that PD did not affect the ATPase activity of Hsp90. However, PD disrupted the co-chaperone interaction of Hsp90/cell division cycle protein 37 (Cdc37) and subsequently degraded multiple Hsp90 client proteins without the feedback increase of Hsp70...
July 12, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28709724/a-probiotic-bacillus-strain-containing-amorphous-poly-beta-hydroxybutyrate-phb-stimulates-the-innate-immune-response-of-penaeus-monodon-postlarvae
#12
Joseph Leopoldo Q Laranja, Edgar C Amar, Gladys L Ludevese-Pascual, Yufeng Niu, Mary Joy Geaga, Peter De Schryver, Peter Bossier
In this study, the PHB-accumulating Bacillus sp. JL47 strain (capable of accumulating 55% PHB on cell dry weight) was investigated for its effects on the immune response of giant tiger shrimp (Penaeus monodon) postlarvae (PL) before and after the Vibrio campbellii challenge. Briefly, shrimp PL were cultured and fed with Artemia nauplii enriched with Bacillus sp. JL47. Shrimp receiving the Artemia nauplii without JL47 enrichment were used as control. After 15 days of feeding, the shrimp were challenged with pathogenic V...
July 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28706419/intestinal-anti-inflammatory-activity-of-ground-cherry-physalis-angulata-l-standardized-co2-phytopharmaceutical-preparation
#13
Luiz Domingues Almeida Junior, Ana Elisa Valencise Quaglio, Celso Acácio Rodrigues de Almeida Costa, Luiz Claudio Di Stasi
AIM: To investigate the effects of Ground Cherry (Physalis angulata L.) standardized supercritical CO2 extract in trinitrobenzenesulphonic acid (TNBS) model of rat intestinal inflammation. METHODS: The animals were divided into groups that received vehicle or P. angulata extract (PACO2) orally at the doses 25, 50 and 100 mg/kg daily by 5 d before TNBS damage. Protective effects of PACO2 were assessed by macroscopic analysis, biochemical determinations of the levels of myeloperoxidase (MPO), alkaline phosphatase (ALP), glutathione and cytokines (such as INF-γ, IL-1β, IL-6, IL-10 and TNF-α), gene expression evaluation (including Hsp70, heparanase, NF-κB, mitogen-activated protein kinases (Mapk) 1, 3, 6 and 9, and the mucins genes Muc 1, 2, 3 and 4) and histopathological studies using optical, and electronic (transmission and scanning) microscopy...
June 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28704482/proteomic-identification-of-proteins-differentially-expressed-following-overexpression-of-htert-human-telomerase-reverse-transcriptase-in-cancer-cells
#14
Rishi Kumar Jaiswal, Pramod Kumar, Amod Sharma, Deepak Kumar Mishra, Pramod Kumar Yadava
Reverse transcriptase activity of telomerase adds telomeric repeat sequences at extreme ends of the newly replicated chromosome in actively dividing cells. Telomerase expression is not detected in terminally differentiated cells but is noticeable in 90% of the cancer cells. hTERT (human telomerase reverse transcriptase) expression seems to promote invasiveness of cancer cells. We here present proteomic profiles of cells overexpressing or knocked down for hTERT. This study also attempts to find out the potential interacting partners of hTERT in cancer cell lines...
2017: PloS One
https://www.readbyqxmd.com/read/28699903/molecular-chaperones-in-the-acquisition-of-cancer-cell-chemoresistance-with-mutated-tp53-and-mdm2-up-regulation
#15
Zuzanna Tracz-Gaszewska, Marta Klimczak, Przemyslaw Biecek, Marcin Herok, Marcin Kosinski, Maciej B Olszewski, Patrycja Czerwińska, Milena Wiech, Maciej Wiznerowicz, Alicja Zylicz, Maciej Zylicz, Bartosz Wawrzynow
Utilizing the TCGA PANCAN12 dataset we discovered that cancer patients with mutations in TP53 tumor suppressor and overexpression of MDM2 oncogene exhibited decreased survival post treatment. Interestingly, in the case of breast cancer patients, this phenomenon correlated with high expression level of several molecular chaperones belonging to the HSPA, DNAJB and HSPC families. To verify the hypothesis that such a genetic background may promote chaperone-mediated chemoresistance, we employed breast and lung cancer cell lines that constitutively overexpressed heat shock proteins and have shown that HSPA1A/HSP70 and DNAJB1/HSP40 facilitated the binding of mutated p53 to the TAp73α protein...
June 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28691182/hsp70-inhibitors-suppress-androgen-receptor-expression-in-lncap95-prostate-cancer-cells
#16
Kazuaki Kita, Masayuki Shiota, Masako Tanaka, Asuka Otsuka, Masaki Matsumoto, Minoru Kato, Satoshi Tamada, Hiroshi Iwao, Katsuyuki Miura, Tatsuya Nakatani, Shuhei Tomita
Androgen deprivation therapy (ADT) is initially effective for treating patients with advanced prostate cancer; however, the prostate cancer gradually becomes resistant to ADT, which is termed castration-resistant prostate cancer (CRPC). Androgen receptor splice variant 7 (AR-V7), one of the causes of CRPC, is correlated with the resistance to a new-generation AR antagonist (enzalutamide) and poor prognosis. Heat shock protein 70 (Hsp70) inhibitor is known to decrease the levels of full-length AR (AR-FL), but little is known about its effects against CRPC cells expressing AR-V7...
July 10, 2017: Cancer Science
https://www.readbyqxmd.com/read/28689711/differential-expression-of-stress-candidate-genes-for-thermal-tolerance-in-the-sea-urchin-loxechinus-albus
#17
Jonathan Vergara-Amado, Andrea X Silva, Catalina Manzi, Roberto F Nespolo, Leyla Cárdenas
Marine ectotherms inhabiting intertidal and shallow subtidal environments are continuously exposed to diurnal tidal cycles and seasonal variability in temperature. These organisms have adaptive mechanisms to maintain cellular homeostasis, irrespective of thermal environmental variation. In this study, we describe the molecular responses to thermal stress in the edible sea urchin Loxechinus albus. In particular, we determined the differential expression of a set of molecular markers that have been identified as targets of stress-related responses...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28687176/the-impact-of-a-single-bout-of-high-intensity-circuit-training-on-myokines-concentrations-and-cognitive-functions-in-women-of-different-age
#18
A Gmiat, K Micielska, M Kozłowska, D J Flis, M Smaruj, S Kujach, J Jaworska, P Lipińska, E Ziemann
The study aimed to assess effect of a single bout of high-intensity circuit training (HICT) on myokines concentration: interleukin-6 and irisin, inteleukin-10, brain-derived neurotrophic factor (BDNF), heat shock proteins (HSP27, HSP70) and cognitive functions among women participated in HICT. It also attempted evaluating whether vitamin D could have modified the effect of HICT. Fourteen healthy, non-active women participating in the experiment were assigned to a young or middle-aged group. They performed a single session HICT using body weight as a resistance, based on the ACSM recommendations...
July 5, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28686685/comparative-proteomic-analysis-of-gib2-validating-its-adaptor-function-in-cryptococcus-neoformans
#19
Gillian O Bruni, Blake Battle, Ben Kelly, Zhengguang Zhang, Ping Wang
Cryptococcus neoformans causes often-fatal fungal meningoencephalitis in immunocompromised individuals. While the exact disease mechanisms remain elusive, signal transduction pathways mediated by key elements such as G-protein α subunit Gpa1, small GTPase Ras1, and atypical Gβ-like/RACK1 protein Gib2 are known to play important roles in C. neoformans virulence. Gib2 is important for normal growth, differentiation, and pathogenicity, and it also positively regulates cAMP levels in conjunction with Gpa1. Interestingly, Gib2 displays a scaffold protein property by interacting with a wide variety of cellular proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28686674/hsf1-and-hsf3-cooperatively-regulate-the-heat-shock-response-in-lizards
#20
Ryosuke Takii, Mitsuaki Fujimoto, Yuki Matsuura, Fangxu Wu, Namiko Oshibe, Eiichi Takaki, Arpit Katiyar, Hiroshi Akashi, Takashi Makino, Masakado Kawata, Akira Nakai
Cells cope with temperature elevations, which cause protein misfolding, by expressing heat shock proteins (HSPs). This adaptive response is called the heat shock response (HSR), and it is regulated mainly by heat shock transcription factor (HSF). Among the four HSF family members in vertebrates, HSF1 is a master regulator of HSP expression during proteotoxic stress including heat shock in mammals, whereas HSF3 is required for the HSR in birds. To examine whether only one of the HSF family members possesses the potential to induce the HSR in vertebrate animals, we isolated cDNA clones encoding lizard and frog HSF genes...
2017: PloS One
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