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https://www.readbyqxmd.com/read/29124751/detailed-characterization-of-human-mycobacterium-tuberculosis-specific-hla-e-restricted-cd8-t-cells
#1
Teresa Prezzemolo, Krista E van Meijgaarden, Kees Lmc Franken, Nadia Caccamo, Francesco Dieli, Tom Hm Ottenhoff, Simone A Joosten
HLA-E presented antigens are interesting targets for vaccination given HLA-Es' essentially monomorphic nature. We have shown previously that Mycobacterium tuberculosis (Mtb) peptides are presented by HLA-E to CD8(+) effector T-cells, but the precise phenotype and functional capacity of these cells remains poorly characterized. We have developed and utilized in this study a new protocol combining HLA-E tetramer with intracellular staining for cytokines, transcription factors and cytotoxic molecules to characterize these cells in depth...
November 9, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29114247/elusive-role-of-the-cd94-nkg2c-nk-cell-receptor-in-the-response-to-cytomegalovirus-novel-experimental-observations-in-a-reporter-cell-system
#2
Aldi Pupuleku, Marcel Costa-García, Domènec Farré, Hartmut Hengel, Ana Angulo, Aura Muntasell, Miguel López-Botet
Human cytomegalovirus (HCMV) infection promotes the differentiation and persistent expansion of a mature NK cell subset, which displays high surface levels of the activating CD94/NKG2C NK cell receptor, together with additional distinctive phenotypic and functional features. The mechanisms underlying the development of adaptive NK cells remain uncertain but some observations support the involvement of a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV-infected cells. To approach this issue, the heterodimer and its adaptor (DAP12) were expressed in the human Jurkat leukemia T cell line; signaling was detected by transfection of a reporter plasmid encoding for Luciferase (Luc) under NFAT/AP1-dependent control...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29098351/the-roles-of-genetic-factors-in-kawasaki-disease-a-systematic-review-and-meta-analysis-of-genetic-association-studies
#3
REVIEW
Xiaochuan Xie, Xiaohan Shi, Meilin Liu
This systematic review and meta-analysis aimed to better elucidate the roles of genetic factors in Kawasaki disease (KD), and determine the potential genetic biomarkers of KD. The systematic literature search of PubMed, Medline, Embase, Web of Science and CNKI identified 164 eligible studies. The qualitative synthesis revealed that 62 genes may be correlated with the susceptibility to KD, and 47 genes may be associated with the incidence of coronary artery lesions (CALs) in KD. A total of 53 polymorphisms in 34 genes were investigated in further quantitative synthesis...
November 2, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/29073617/preimplantation-factor-pif-promotes-hla-g-e-f-c-expression-in-jeg-3-choriocarcinoma-cells-and-endogenous-progesterone-activity
#4
Miya Soukaina Hakam, Jose Maria Miranda-Sayago, Soren Hayrabedyan, Krassimira Todorova, Patrick Simon Spencer, Asma Jabeen, Eytan R Barnea, Nelson Fernandez
BACKGROUND/AIMS: Pregnancy success requires mandatory maternal tolerance of the semi/ allogeneic embryo involving embryo-derived signals. Expression levels of PreImplantation Factor (PIF), a novel peptide secreted by viable embryos, correlate with embryo development, and its early detection in circulation correlates with a favourable pregnancy outcome. PIF enhances endometrial receptivity to promote embryo implantation. Via the p53 pathway, it increases trophoblast invasion, improving cell survival / immune privilege...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29059634/the-prognostic-benefit-of-tumour-infiltrating-natural-killer-cells-in-endometrial-cancer-is-dependent-on-concurrent-overexpression-of-human-leucocyte-antigen-e-in-the-tumour-microenvironment
#5
M A C Versluis, S Marchal, A Plat, G H de Bock, T van Hall, M de Bruyn, H Hollema, H W Nijman
BACKGROUND: Human Leucocyte Antigen- E (HLA-E) has been reported as both a positive and negative prognostic marker in cancer. This apparent discrepancy may be due to opposing actions of HLA-E on tumour-infiltrating immune cells. Therefore, we evaluated HLA-E expression and survival in relation to the presence of intratumoural natural killer (NK) cells and cytotoxic T cells (CTLs). METHODS: Tissue microarrays (TMAs) of endometrial tumours were used for immunohistochemical staining of parameters of interest...
October 20, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/29057510/release-of-pig-leukocytes-and-reduced-human-nk-cell-recruitment-during-ex-vivo-perfusion-of-hla-e-human-cd46-double-transgenic-pig-limbs-with-human-blood
#6
Gisella Puga Yung, Anjan K Bongoni, Amandine Pradier, Natacha Madelon, Maria Papaserafeim, Riccardo Sfriso, David L Ayares, Eckhard Wolf, Nikolai Klymiuk, Andrea Bähr, Mihai A Constantinescu, Esther Voegelin, David Kiermeir, Hansjörg Jenni, Robert Rieben, Jörg D Seebach
BACKGROUND: In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E (HLA-E) on pEC. Thus, the aim of this study was to analyze NK cell responses to vascularized xenografts using an ex vivo perfusion system of pig limbs with human blood...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29023371/expression-profiles-of-ligands-for-activating-natural-killer-cell-receptors-on-hiv-infected-and-uninfected-cd4%C3%A2-%C2%BA-t-cells
#7
Alexandra Tremblay-McLean, Julie Bruneau, Bertrand Lebouché, Irene Lisovsky, Rujun Song, Nicole F Bernard
Natural Killer (NK) cell responses to HIV-infected CD4 T cells (iCD4) depend on the integration of signals received through inhibitory (iNKR) and activating NK receptors (aNKR). iCD4 activate NK cells to inhibit HIV replication. HIV infection-dependent changes in the human leukocyte antigen (HLA) ligands for iNKR on iCD4 are well documented. By contrast, less is known regarding the HIV infection related changes in ligands for aNKR on iCD4. We examined the aNKR ligand profiles HIV p24⁺ HIV iCD4s that maintained cell surface CD4 (iCD4⁺), did not maintain CD4 (iCD4(-)) and uninfected CD4 (unCD4) T cells for expression of unique long (UL)-16 binding proteins-1 (ULBP-1), ULBP-2/5/6, ULBP-3, major histocompatibility complex (MHC) class 1-related (MIC)-A, MIC-B, CD48, CD80, CD86, CD112, CD155, Intercellular adhesion molecule (ICAM)-1, ICAM-2, HLA-E, HLA-F, HLA-A2, HLA-C, and the ligands to NKp30, NKp44, NKp46, and killer immunoglobulin-like receptor 3DS1 (KIR3DS1) by flow cytometry on CD4 T cells from 17 HIV-1 seronegative donors activated and infected with HIV...
October 12, 2017: Viruses
https://www.readbyqxmd.com/read/28979268/nkg2c-nkg2a-natural-killer-cells-are-associated-with-a-lower-viral-set-point-and-may-predict-disease-progression-in-individuals-with-primary-hiv-infection
#8
Meichen Ma, Zhuo Wang, Xi Chen, Anfu Tao, Lei He, Shuai Fu, Zining Zhang, Yajing Fu, Chenxi Guo, Jing Liu, Xiaoxu Han, Junjie Xu, Zhenxing Chu, Haibo Ding, Hong Shang, Yongjun Jiang
Natural killer (NK) cells are the first line of defense against pathogens of the immune system and also play an important role in resistance against HIV. The activating receptor NKG2C and the inhibitory receptor NKG2A co-modulate the function of NK cells by recognizing the same ligand, HLA-E. However, the role of NKG2A and NKG2C on viral set point and the prediction of HIV disease progression have been rarely reported. In this study, we determined the expression of NKG2C or NKG2A on the surface of NK cells from 22 individuals with primary HIV infection (PHI) stage and 23 HIV-negative normal control (NC) subjects...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28972140/a-conserved-energetic-footprint-underpins-recognition-of-human-leukocyte-antigen-e-by-two-distinct-%C3%AE-%C3%AE-t-cell-receptors
#9
Lucy C Sullivan, Nicholas G Walpole, Carine Farenc, Gabriella Pietra, Matthew J Wenhwa Sum, Craig S Clements, Eleanor J Lee, Travis Beddoe, Michela Falco, Maria Cristina Mingari, Lorenzo Moretta, Stephanie Gras, Jamie Rossjohn, Andrew G Brooks
αβ T cell receptors (TCRs) interact with peptides bound to the polymorphic Major Histocompatibility Complex class Ia (MHC-Ia) and class II (MHC-II) molecules, as well as the essentially monomorphic MHC class Ib (MHC-Ib) molecules. While there is a large amount of information on how TCRs engage with MHC-Ia and MHC-II, our understanding of TCR-MHC-Ib interactions is very limited. Infection with cytomegalovirus (CMV) can elicit a CD8+ T cell response restricted by the human MHC-Ib molecule, Human Leukocyte Antigen (HLA)-E, and specific for an epitope from UL40 (VMAPRTLIL), which is characterized by biased TRBV14 gene usage...
September 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28946074/hla-e-regulatory-and-coding-region-variability-and-haplotypes-in-a-brazilian-population-sample
#10
Jaqueline Ramalho, Luciana C Veiga-Castelli, Eduardo A Donadi, Celso T Mendes-Junior, Erick C Castelli
The HLA-E gene is characterized by low but wide expression on different tissues. HLA-E is considered a conserved gene, being one of the least polymorphic class I HLA genes. The HLA-E molecule interacts with Natural Killer cell receptors and T lymphocytes receptors, and might activate or inhibit immune responses depending on the peptide associated with HLA-E and with which receptors HLA-E interacts to. Variable sites within the HLA-E regulatory and coding segments may influence the gene function by modifying its expression pattern or encoded molecule, thus, influencing its interaction with receptors and the peptide...
November 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28904123/unveiling-the-peptide-motifs-of-hla-c-and-hla-g-from-naturally-presented-peptides-and-generation-of-binding-prediction-matrices
#11
Moreno Di Marco, Heiko Schuster, Linus Backert, Michael Ghosh, Hans-Georg Rammensee, Stefan Stevanović
The classical HLA-C and the nonclassical HLA-E and HLA-G molecules play important roles both in the innate and adaptive immune system. Starting already during embryogenesis and continuing throughout our lives, these three Ags exert major functions in immune tolerance, defense against infections, and anticancer immune responses. Despite these important roles, identification and characterization of the peptides presented by these molecules has been lacking behind the more abundant HLA-A and HLA-B gene products...
October 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28899983/regulatory-nk-cells-mediated-between-immunosuppressive-monocytes-and-dysfunctional-t-cells-in-chronic-hbv-infection
#12
Haijun Li, Naicui Zhai, Zhongfeng Wang, Hongxiao Song, Yang Yang, An Cui, Tianyang Li, Guangyi Wang, Junqi Niu, Ian Nicholas Crispe, Lishan Su, Zhengkun Tu
BACKGROUND AND AIMS: HBV infection represents a major health problem worldwide, but the immunological mechanisms by which HBV causes chronic persistent infection remain only partly understood. Recently, cell subsets with suppressive features have been recognised among monocytes and natural killer (NK) cells. Here we examine the effects of HBV on monocytes and NK cells. METHODS: Monocytes and NK cells derived from chronic HBV-infected patients and healthy controls were purified and characterised for phenotype, gene expression and cytokines secretion by flow cytometry, quantitative real-time (qRT)-PCR, ELISA and western blotting...
September 12, 2017: Gut
https://www.readbyqxmd.com/read/28883078/human-leukocyte-antigen-e-mismatch-is-associated-with-better-hematopoietic-stem-cell-transplantation-outcome-in-acute-leukemia-patients
#13
Chrysanthi Tsamadou, Daniel Fürst, Vladan Vucinic, Donald Bunjes, Christine Neuchel, Daphne Mytilineos, Martin Gramatzki, Renate Arnold, Eva Maria Wagner, Hermann Einsele, Carlheinz Müller, Hubert Schrezenmeier, Joannis Mytilineos
The immunomodulatory role of human leukocyte antigen (HLA)-E in hematopoietic stem cell transplantation (HSCT) has not been extensively investigated. To this end, we genotyped 509 10/10 HLA unrelated transplant pairs for HLA-E, in order to study the effect of HLA-E as a natural killer (NK)-alloreactivity mediator on HSCT outcome in an acute leukemia (AL) setting. Overall survival (OS), disease free survival (DFS), relapse incidence (RI) and non-relapse mortality (NRM) were set as endpoints. Analysis of our data revealed a significant correlation between HLA-E mismatch and improved HSCT outcome, as shown by both univariate (53% vs 38%, P=0...
November 2017: Haematologica
https://www.readbyqxmd.com/read/28882620/hla-class-ia-and-ib-molecules-and-foxp3-tils-in-relation-to-the-prognosis-of-malignant-melanoma-patients
#14
Wenna Nascimento Melsted, Lasse Lindholm Johansen, Jørgen Lock-Andersen, Nille Behrendt, Jens Ole Eriksen, Michael Bzorek, Thomas Scheike, Thomas Vauvert F Hviid
HLA class Ia (HLA-ABC) and HLA class Ib (HLA-E, -F and -G) molecules and FOXP3+ tumor-infiltrating lymphocytes (TILs) are often reported as relevant factors of tumor immune regulation. We investigated their expression as prognostic factors in 200 patients with primary cutaneous melanoma (PCM). In our cohort, patients with tumors showing upregulation of HLA-ABC molecules had significantly thicker tumors (32% vs 7%, P<0.001), frequent ulceration (20% vs 6%, P=0.007) and frequent nodular melanomas (20% vs 4%, P=0...
September 4, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28874155/the-regulation-of-ovary-and-conceptus-on-the-uterine-natural-killer-cells-during-early-pregnancy
#15
REVIEW
Han Gong, Yilu Chen, Jingjie Xu, Xingxing Xie, Dainan Yu, Bei Yang, Haibin Kuang
Uterine natural killer (uNK) cells are short-lived, terminally differentiated and the most abundant lymphocytes in the uterus which play a crucial role in the spiral arteriole modification and establishment of successful pregnancy. Dysregulation of uNK cells has been linked to gestational implications such as recurrent pregnancy loss, preeclampsia and fetal growth retardation. There is evidence showing that progesterone and estrogen can regulate the recruitment, proliferation, differentiation and function of uNK cells via direct action on intracellular nuclear receptors or through intermediary cells in the uterus during early pregnancy...
September 6, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28783683/class-i-hla-haplotypes-form-two-schools-that-educate-nk-cells-in-different-ways
#16
Amir Horowitz, Zakia Djaoud, Neda Nemat-Gorgani, Jeroen Blokhuis, Hugo G Hilton, Vivien Béziat, Karl-Johan Malmberg, Paul J Norman, Lisbeth A Guethlein, Peter Parham
Natural killer (NK) cells are lymphocytes that have vital functions in innate and adaptive immunity, as well as placental reproduction. Polymorphic human leukocyte antigen (HLA) class I educates NK cells through interactions with killer cell immunoglobulin-like receptors (KIRs) and by supplying peptides that bind HLA-E to form ligands for CD94/NKG2A receptors on NK cells. HLA-B dimorphism in the leader peptide modulates this latter function: -21methionine (-21M) delivers functional peptides, but -21threonine (-21T) does not...
September 9, 2016: Science Immunology
https://www.readbyqxmd.com/read/28769934/human-leukocyte-antigen-hla-and-immune-regulation-how-do-classical-and-non-classical-hla-alleles-modulate-immune-response-to-human-immunodeficiency-virus-and-hepatitis-c-virus-infections
#17
REVIEW
Nicole B Crux, Shokrollah Elahi
The genetic factors associated with susceptibility or resistance to viral infections are likely to involve a sophisticated array of immune response. These genetic elements may modulate other biological factors that account for significant influence on the gene expression and/or protein function in the host. Among them, the role of the major histocompatibility complex in viral pathogenesis in particular human immunodeficiency virus (HIV) and hepatitis C virus (HCV), is very well documented. We, recently, added a novel insight into the field by identifying the molecular mechanism associated with the protective role of human leukocyte antigen (HLA)-B27/B57 CD8(+) T cells in the context of HIV-1 infection and why these alleles act as a double-edged sword protecting against viral infections but predisposing the host to autoimmune diseases...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28727628/replication-confirms-the-association-of-loci-in-foxe1-pde8b-capzb-and-pde10a-with-thyroid-traits-a-genetics-of-diabetes-audit-and-research-tayside-study-godarts
#18
Enrique Soto-Pedre, Moneeza K Siddiqui, Alex S Doney, Colin N A Palmer, Ewan R Pearson, Graham P Leese
OBJECTIVE: Replication of associations in genome-wide association studies is desirable to ensure that such signals are potentially clinically meaningful. This study aimed to replicate associations of selected single-nucleotide polymorphisms (SNPs) with hypothyroidism and serum thyroid-stimulating hormone (TSH) using electronic medical records (EMRs). PATIENTS AND METHODS: A cross-sectional study was carried out among patients of European Caucasian ethnicity from the Genetics of Diabetes Audit and Research Tayside recruited in Tayside (Scotland, UK)...
October 2017: Pharmacogenetics and Genomics
https://www.readbyqxmd.com/read/28711734/immunohistochemical-investigations-on-the-expression-of-programmed-cell-death-ligand-1-human-leukocyte-antigens-g-and-e-and-granzyme-b-in-intraoral-mucoepidermoid-carcinoma
#19
Carla Mosconi, Diego Antônio Costa Arantes, Andréia Souza Gonçalves, Rita de Cássia Gonçalves Alencar, José Carlos Oliveira, Tarcília Aparecida Silva, Elismauro Francisco Mendonça, Aline Carvalho Batista
OBJECTIVE: To identify the expression of nonclassical human leukocyte antigen G and E (HLA-G and -E), programmed cell death ligand-1 (PD-L1) and granzyme B (GB) in intraoral mucoepidermoid carcinomas (MECs), and to assess whether such expressions are related to metastasis, survival, staging, tumor grade and number of GB-positive cells. DESIGN: For this cross-sectional study, samples of MEC (n=30) were selected and classified as low-grade (LG), intermediate-grade (IG) or high-grade (HG), according to the WHO grading system...
July 8, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28699111/hla-class-ib-in-pregnancy-and-pregnancy-related-disorders
#20
REVIEW
Gry Persson, Wenna Nascimento Melsted, Line Lynge Nilsson, Thomas Vauvert F Hviid
The HLA class Ib genes, HLA-E, HLA-F, and HLA-G, were discovered long after the classical HLA class Ia genes. The elucidation of their functions had a modest beginning. However, their basic functions and involvement in pathophysiology and a range of diseases are now emerging. Although results from a range of studies support the functional roles for the HLA class Ib molecules in adult life, especially HLA-G and HLA-F have most intensively been, and were also primarily, studied in relation to reproduction and pregnancy...
August 2017: Immunogenetics
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