keyword
MENU ▼
Read by QxMD icon Read
search

transglutaminase

keyword
https://www.readbyqxmd.com/read/28327670/global-identification-and-analysis-of-isozyme-specific-possible-substrates-crosslinked-by-transglutaminases-using-substrate-peptides-in-mouse-liver-fibrosis
#1
Hideki Tatsukawa, Yuji Tani, Risa Otsu, Haruka Nakagawa, Kiyotaka Hitomi
The transglutaminase (TG) family comprises eight isozymes that form the isopeptide bonds between glutamine and lysine residues and contribute to the fibrotic diseases via crosslinking-mediated stabilization of ECM and the activation of TGF-β in several tissues. However, despite a growing body of evidence implicating TG2 as a key enzyme in fibrosis, the causative role of TG2 and the involvement of the other isozymes have not yet been fully elucidated. Therefore, here we clarified the distributions of TG isozymes and their in situ activities and identified the isozyme-specific possible substrates for both TG1 and TG2 using their substrate peptides in mouse fibrotic liver...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28321657/novel-hemostatic-agents-based-on-gelatin-microbial-transglutaminase-mix
#2
Fang Lv, Xiaonan Cong, Wenshu Tang, Yiming Han, Yu Tang, Yongrui Liu, Liqiang Su, Mingyao Liu, Mingfei Jin, Zhengfang Yi
Hemostasis is a major challenge in surgical procedures and traumas. Conventional hemostatic methods have limited efficacy and may cause additional tissue damage. In this study, we designed a novel hemostatic agent based on the in situ gel formation of gelatin cross-linked by a novel microbial transglutaminase (mTGase), in which the amino acid sequences differed from commercial mTGases. The new hemostatic agent showed the same biochemical crosslinking chemistry as the final stages of the blood coagulation cascade while using gelatin as a "structural" protein (rather than fibrin) and a calcium-independent mTGase as the crosslinking catalyst (rather than factor XIIIa)...
March 17, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28321610/correlation-of-tissue-transglutaminase-with-modified-marsh-grading-in-celiac-disease-a-prospective-cohort-study
#3
Rakesh Jora, Vikrant Raghuvanshi, Vikas Payal, Pramod Sharma, Shree Krishan Vishnoi
OBJECTIVE: To find out correlation between serum anti-tissue transglutaminase immunoglobulin-A (tTGA) levels and Marsh grading on duodenal histopathology in Celiac disease (CD). METHODS: In a prospective cohort study, a total of 52 symptomatic patients between age group of 2-18 y were enroled. All enroled patients were subjected to upper GI endoscopy by an experienced endoscopist. Two biopsies each from the bulb (D1) and second part (D2) of the duodenum were taken and Marsh grading was performed by a single experienced pathologist...
March 21, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28320098/inhibition-of-tissue-transglutaminase-attenuates-lipopolysaccharide-induced-inflammation-in-glial-cells-through-akt-mtor-signal-pathway
#4
Yirong Ding, Ji Zhang, Rui Wang
AIM: In view of the facts that tTG protein expression level and its enzyme activity increase in AD brains of both individuals and transgenic animals and compelling evidence of the involvement of inflammation in AD pathogenesis, tTG could be involved in the inflammation responses in the brain. In the present study, we examined the effects of the irreversible and the competitive inhibitor of tTG on the condition of lipopolysaccharide-induced mimic inflammation models in glial cells. METHODS: Western blot and tTG enzyme activity assay were applied to detect tTG and isopeptide protein levels and tTG enzyme activity...
March 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28315658/transglutaminase-3-protects-against-photodamage
#5
Valentina Frezza, Alessandro Terrinoni, Consuelo Pitolli, Alessandro Mauriello, Gerry Melino, Eleonora Candi
No abstract text is available yet for this article.
March 15, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28315501/mechanical-properties-and-biocompatibility-of-in-situ-enzymatically-cross-linked-gelatin-hydrogels
#6
Nada Z Alarake, Patrick Frohberg, Thomas Groth, Markus Pietzsch
OBJECTIVES: Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues. METHODS: Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C...
March 18, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28289500/olmesartan-induced-enteropathy
#7
Abimbola Adike, Juan Corral, David Rybnicek, Daniel Sussman, Samir Shah, Eamonn Quigley
Olmesartan-induced enteropathy mimics celiac disease clinically and pathologically. As in celiac disease, the pathologic findings are villous atrophy and increased intraepithelial lymphocytes. Clinical presentation of olmesartan-induced enteropathy includes diarrhea, weight loss, and nausea. In contrast to celiac disease, tissue transglutaminase is not elevated and there is no response to a gluten-free diet. Including this entity in the differential diagnosis of sprue-like enteropathy is critical for its early diagnosis since replacing olmesartan with an alternative antihypertensive drug can simplify the diagnostic workup and provide both clinical and histologic improvement...
October 2016: Methodist DeBakey Cardiovascular Journal
https://www.readbyqxmd.com/read/28289145/irf6-promotes-keratinocyte-differentiation-in-response-to-porphyromonas-gingivalis
#8
Jennifer Huynh, Glen M Scholz, Jiamin Aw, Eric C Reynolds
We recently demonstrated that the expression of the Interferon regulatory factor 6 (IRF6) transcription factor in oral keratinocytes was stimulated by the periodontal pathogen Porphyromonas gingivalis Here, we have established that IRF6 promotes the differentiation of oral keratinocytes in response to P. gingivalis This was evidenced by the IRF6-dependent upregulation of specific markers of keratinocyte terminal differentiation (e.g. Involucrin, IVL; Keratin 13, KRT13), together with additional transcriptional regulators of keratinocyte differentiation, including Grainyhead-like 3 (GRHL3) and Ovo-like zinc finger 1 (OVOL1)...
March 13, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28284699/incorporated-glucosamine-adversely-affects-the-emulsifying-properties-of-whey-protein-isolate-polymerized-by-transglutaminase
#9
Lin Chen, Niamat Ullah, Chenyi Li, Robert M Hackman, Zhixi Li, Xinglian Xu, Guanghong Zhou, Xianchao Feng
Glucosamine (GlcN) and microbial transglutaminase (Tgase) are used separately or together to improve the emulsifying properties of whey protein isolate (WPI). However, little is known about how the emulsifying properties change when GlcN residues are incorporated into WPI cross-linked by Tgase. We used Tgase as a biocatalyst to cross-link WPI in the presence of GlcN in a liquid system for 12 h at a moderate temperature (25°C). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses indicated that protein polymerization and GlcN conjugation occurred simultaneously, phenomena also supported by the loss of free amines (9...
March 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28276604/one-step-immunochromatographic-visual-assay-for-anti-transglutaminase-detection-in-organ-culture-system-an-easy-and-prompt-method-to-simplify-the-in-vitro-diagnosis-of-celiac-disease
#10
Marco Di Tola, Mariacatia Marino, Rossella Casale, Raffaele Borghini, Antonio Tiberti, Giuseppe Donato, Umberto Occhiuzzi, Antonio Picarelli
BACKGROUND: Anti-tissue transglutaminase (anti-tTG) and endomysium antibodies (EMA) are detectable in duodenal culture media of celiac disease (CD) patients. To improve the management of this organ culture system, we evaluated the anti-tTG occurrence by immunochromatographic assay (ICA). METHODS: A total of 103 CD patients and 41 disease controls underwent duodenal biopsy for the organ culture. In culture supernatants, IgA anti-tTG were tested by both enzyme-linked immunosorbent assay (ELISA) and ICA, IgA EMA were searched by indirect immunofluorescence analysis (iIFA)...
March 9, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28274441/preparation-of-glycosylated-zein-and-retarding-effect-on-lipid-oxidation-of-ground-pork
#11
Xiao-Jie Wang, Xi-Qun Zheng, Xiao-Lan Liu, Narasimha-Kumar Kopparapu, Wan-Suo Cong, Yong-Ping Deng
The focus of the present work was to investigate the glycosylation of zein, partial properties of the glycosylated zein (GZ) and its retarding effect on lipid oxidation of ground pork. Zein was glycosylated with chitosan (MW 1500Da) by microbial transglutaminase, the reaction was verified by FT-IR. Under the optimized conditions, 97.48mg of glucosamine was covalently conjugated to 1g of zein, determined by HPLC. The solubility and the surface hydrophobicity of GZ were significantly improved. In vitro studies of GZ showed a dose-dependent scavenging activity against free radicals of DPPH, superoxide and hydroxyl radical, and the EC50 value for DPPH radical was 1...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28273770/transglutaminase-6-is-an-autoantigen-in-progressive-multiple-sclerosis-and-is-upregulated-in-reactive-astrocytes
#12
Massimiliano Cristofanilli, Daniel Gratch, Benjamin Pagano, Kelsey McDermott, Jessie Huang, Jeffrey Jian, Deneb Bates, Saud A Sadiq
BACKGROUND: Transglutaminase-6 (TGM6), a member of the transglutaminase enzyme family, is found predominantly in central nervous system (CNS) neurons under physiological conditions. It has been proposed as an autoimmune target in cerebral palsy, gluten-sensitive cerebellar ataxia, and schizophrenia. OBJECTIVE: To investigate TGM6 involvement in multiple sclerosis (MS). METHODS: Antibody levels against TGM6 (TGM6-IgG) were measured in the cerebrospinal fluid (CSF) of 62 primary progressive multiple sclerosis (PPMS), 85 secondary progressive multiple sclerosis (SPMS), and 50 relapsing-remitting multiple sclerosis (RRMS) patients and 51 controls...
December 1, 2016: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28267644/production-of-dry-cured-formed-ham-with-different-concentrations-of-microbial-transglutaminase-mass-spectrometric-analysis-and-sensory-evaluation
#13
Wolfgang Jira, Arash Sadeghi-Mehr, Dagmar A Brüggemann, Fredi Schwägele
Dry-cured formed hams were produced with different concentrations of microbial transglutaminase (TG; 0.05, 0.1, 0.2, 0.5, and 0.8% Activa PB) and glucono-delta-lactone as control. A sensory evaluation was performed during a 43-day storage to determine cohesion, cavities, and local separation of dry-cured formed ham. Rising TG concentrations resulted in a slight increase in the evaluation of all sensory parameters, whereas amounts of TG higher than 0.5% led to an only very minor improvement. Dry-cured formed ham samples were analyzed by a sensitive HPLC-MS/MS method for the detection of TG using five tryptic marker peptides...
February 24, 2017: Meat Science
https://www.readbyqxmd.com/read/28264651/interactions-of-histatin-3-and-histatin-5-with-actin
#14
Edna Blotnick, Asaf Sol, Gilad Bachrach, Andras Muhlrad
BACKGROUND: Histatins are histidine rich polypeptides produced in the parotid and submandibular gland and secreted into the saliva. Histatin-3 and -5 are the most important polycationic histatins. They possess antimicrobial activity against fungi such as Candida albicans. Histatin-5 has a higher antifungal activity than histatin-3 while histatin-3 is mostly involved in wound healing in the oral cavity. We found that these histatins, like other polycationic peptides and proteins, such as LL-37, lysozyme and histones, interact with extracellular actin...
March 6, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28262745/a-biomimetic-gelatin-based-platform-elicits-a-pro-differentiation-effect-on-podocytes-through-mechanotransduction
#15
Mufeng Hu, Evren U Azeloglu, Amit Ron, Khanh-Hoa Tran-Ba, Rhodora C Calizo, Iman Tavassoly, Smiti Bhattacharya, Gomathi Jayaraman, Yibang Chen, Vera Rabinovich, Ravi Iyengar, James C Hone, John C He, Laura J Kaufman
Using a gelatin microbial transglutaminase (gelatin-mTG) cell culture platform tuned to exhibit stiffness spanning that of healthy and diseased glomeruli, we demonstrate that kidney podocytes show marked stiffness sensitivity. Podocyte-specific markers that are critical in the formation of the renal filtration barrier are found to be regulated in association with stiffness-mediated cellular behaviors. While podocytes typically de-differentiate in culture and show diminished physiological function in nephropathies characterized by altered tissue stiffness, we show that gelatin-mTG substrates with Young's modulus near that of healthy glomeruli elicit a pro-differentiation and maturation response in podocytes better than substrates either softer or stiffer...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28258224/transglutaminase-catalyzed-incorporation-of-polyamines-masks-the-dna-binding-region-of-the-transcription-factor-relish
#16
Kouki Maki, Toshio Shibata, Shun-Ichiro Kawabata
In Drosophila, the immune deficiency (IMD) pathway-dependent signal is finally transmitted through proteolytic conversion of the nuclear factor-κB-like transcription factor Relish to the active N-terminal fragment Relish-N to induce its translocation from the cytosol into the nucleus for the expression of IMD-controlled genes. We previously demonstrated that transglutaminase (TG) suppresses the IMD pathway by polymerizing Relish-N to inhibit its nuclear translocation. On the other hand, we also demonstrated that a synthetic amine, such as monodansylcadaverine (DCA) or biotin-labeled pentylamine, ingested by flies is TG-dependently incorporated into Relish-N, causing the nuclear translocation of modified Relish-N in gut epithelial cells...
March 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28253975/assessing-the-catalytic-activity-of-transglutaminases-in-the-context-of-autophagic-responses
#17
M D'Eletto, M G Farrace, M Piacentini, F Rossin
The human transglutaminases (TGases) are a widely distributed and peculiar group of enzymes that catalyze the posttranslational modification of proteins by the formation of isopeptide bonds. Tissue or type 2 transglutaminase (TG2) represents the most ubiquitous isoform belonging to TGases family. The vast array of biochemical functions catalyzed by TG2 distinguishes it from the other members of the TGase family. In the presence of high calcium levels TG2 catalyzes a vast array of protein posttranslational modifications, including protein-protein cross-linking, incorporation of primary amines into proteins, as well as glutamine deamination...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28250436/towards-celiac-safe-foods-decreasing-the-affinity-of-transglutaminase-2-for-gliadin-by-addition-of-ascorbyl-palmitate-and-zncl2-as-detoxifiers
#18
N Engstrom, P Saenz-Méndez, J Scheers, N Scheers
Initiation of celiac disease is triggered in the gastrointestinal tract by transglutaminase 2 (TG2) assisted deamidation of gluten peptides. Deamidation is a side-reaction to transamidation and occurs if primary amines are absent. In contrast to deamidation, transamidation does not trigger an immune response. The aim of the study was to identify a suitable food additive that interacts with TG2 binding motives in gluten-derived peptides to prevent deamidation/transamidation. Homology modelling of α2-gliadin and computational screening of compounds for their binding affinity to a common TG2 binding motive (P)QLP were done by using computational approaches followed by experimental testing of TG2 activity...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28249640/maternal-use-of-dietary-supplements-during-pregnancy-is-not-associated-with-coeliac-disease-in-the-offspring-the-environmental-determinants-of-diabetes-in-the-young-teddy-study
#19
Jimin Yang, Roy N Tamura, Carin A Aronsson, Ulla M Uusitalo, Åke Lernmark, Marian Rewers, William A Hagopian, Jin-Xiong She, Jorma Toppari, Anette G Ziegler, Beena Akolkar, Jeffrey P Krischer, Jill M Norris, Suvi M Virtanen, Daniel Agardh
Perinatal exposure to nutrients and dietary components may affect the risk for coeliac disease (CD). We investigated the association between maternal use of vitamin D, n-3 fatty acids (FA) and Fe supplements during pregnancy and risk for CD autoimmunity (CDA) and CD in the offspring. Children at increased genetic risk were prospectively followed from birth in The Environmental Determinants of Diabetes in the Young (TEDDY) study. CDA was defined as having persistently positive tissue transglutaminase autoantibodies (tTGA)...
March 2, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28248968/genomic-variants-reveal-differential-evolutionary-constraints-on-human-transglutaminases-and-point-towards-unrecognized-significance-of-transglutaminase-2
#20
Kiruphagaran Thangaraju, Róbert Király, Máté A Demény, János András Mótyán, Mónika Fuxreiter, László Fésüs
Transglutaminases (TGMs) catalyze Ca2+-dependent transamidation of proteins with specified roles in blood clotting (F13a) and in cornification (TGM1, TGM3). The ubiquitous TGM2 has well described enzymatic and non-enzymatic functions but in-spite of numerous studies its physiological function in humans has not been defined. We compared data on non-synonymous single nucleotide variations (nsSNVs) and loss-of-function variants on TGM1-7 and F13a from the Exome aggregation consortium dataset, and used computational and biochemical analysis to reveal the roles of damaging nsSNVs of TGM2...
2017: PloS One
keyword
keyword
35385
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"