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https://www.readbyqxmd.com/read/29475193/site-specific-immobilization-of-endoglycosidases-for-streamlined-chemoenzymatic-glycan-remodeling-of-antibodies
#1
Tiezheng Li, Chao Li, David N Quan, William E Bentley, Lai-Xi Wang
Chemoenzymatic glycan remodeling of antibodies using an endoglycosidase and its mutant is emerging as an attractive approach for producing homogeneous antibody glycoforms. We report in this paper a site-specific covalent immobilization of the endoglycosidases (Endo-S2 and its glycosynthase mutant D184M) using a recombinant microbial transglutaminase (MTG) and evaluation of the immobilized enzymes in deglycosylation and glycosylation of a therapeutic antibody. The site-specific covalent immobilization was achieved by introduction of a Q-tag at the C-terminus of the recombinant enzymes followed by conjugation of the enzymes to a primary amine-containing solid support through MTG-catalyzed transglutamination...
February 15, 2018: Carbohydrate Research
https://www.readbyqxmd.com/read/29473148/a-subset-of-patients-with-pemphigoid-herpes-gestationis-has-serological-evidence-of-celiac-disease
#2
Hedieh Saffari, John J Zone, Marjorie Allen, Kristin M Leiferman
BACKGROUND: Pemphigoid (herpes) gestationis (PG) is an uncommon, self-limited disease with other autoimmune associations; however, celiac disease (CD) is not recognized as one. METHODS: From 71 patients' sera submitted for herpes gestationis factor (HGF) testing over a 5-year period, 12 were consistent with PG demonstrating HGF and increased IgG BP180 antibody levels; these sera were tested for IgA and IgG endomysial antibodies (EMA), epithelial basement membrane zone and cell surface antibodies by indirect immunofluorescence, and for IgA and IgG tissue transglutaminase (transglutaminase 2 or TG2) antibodies, IgA epidermal transglutaminase (transglutaminase 3 or TG3) antibodies, IgG BP230, and IgG desmoglein 1 and desmoglein 3 antibodies by enzyme-linked immunosorbent assays (ELISAs)...
February 23, 2018: International Journal of Dermatology
https://www.readbyqxmd.com/read/29470318/cesarean-section-on-the-risk-of-celiac-disease-in-the-offspring-the-teddy-study
#3
Sibylle Koletzko, Hye-Seung Lee, Andreas Beyerlein, Carin A Aronsson, Michael Hummel, Edwin Liu, Ville Simell, Kalle Kurppa, Åke Lernmark, William Hagopian, Marian Rewers, Jin-Xiong She, Olli Simell, Jorma Toppari, Anette-G Ziegler, Jeffrey Krischer, Daniel Agardh
OBJECTIVE: Cesarean section (C-section) is associated with various immune-mediated diseases in the offspring. We investigated the relationship between mode of delivery and celiac disease (CD) and CD autoimmunity (CDA) in a multinational birth cohort. METHODS: From 2004 to 2010, infants from the general population who tested positive for HLA DR3-DQ2 or DR4-DQ8 were enrolled in The Environmental Determinants for Diabetes in the Young (TEDDY) study. Children were annually screened for transglutaminase autoantibodies, if positive, they are retested after 3 to 6 months and those persistently positive defined as CDA...
March 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29464939/genome-analysis-of-two-novel-pseudomonas-strains-exhibiting-differential-hypersensitivity-reactions-on-tobacco-seedlings-reveals-differences-in-nonflagellar-t3ss-organization-and-predicted-effector-proteins
#4
Caetanie F Tchagang, Renlin Xu, Cyr Lézin Doumbou, James T Tambong
Multilocus sequence analysis (MLSA) of two new biological control strains (S1E40 and S3E12) of Pseudomonas was performed to assess their taxonomic position relative to close lineages, and comparative genomics employed to investigate whether these strains differ in key genetic features involved in hypersensitivity responses (HRs). Strain S3E12, at high concentration, incites HRs on tobacco and corn plantlets while S1E40 does not. Phylogenies based on individual genes and 16S rRNA-gyrB-rpoB-rpoD concatenated sequence data show strains S1E40 and S3E12 clustering in distinct groups...
February 21, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29462712/macrophage-involvement-affects-matrix-stiffness-related-influences-on-cell-osteogenesis-under-three-dimensional-culture-conditions
#5
Xiao-Tao He, Rui-Xin Wu, Xin-Yue Xu, Jia Wang, Yuan Yin, Fa-Ming Chen
Accumulating evidence indicates that the physicochemical properties of biomaterials exert profound influences on stem cell fate decisions. However, matrix-based regulation selected through in vitro analyses based on a given cell population do not genuinely reflect the in vivo conditions, in which multiple cell types are involved and interact dynamically. This study constitutes the first investigation of how macrophages (Mφs) in stiffness-tunable transglutaminase cross-linked gelatin (TG-gel) affect the osteogenesis of bone marrow-derived mesenchymal stem cells (BMMSCs)...
February 17, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29460500/auto-and-alloantibodies-against-factor-xiii-laboratory-diagnosis-and-clinical-consequences
#6
REVIEW
L Muszbek, K Pénzes, É Katona
Acquired FXIII deficiencies caused by autoantibodies against FXIII subunits represent rare but very severe bleeding diatheses. Alloantibodies in FXIII deficient patients also cause life-threatening bleeding complication, but they develop extremely rarely. In this review we provide an overview on the diagnosis and classification of anti-FXIII antibodies and analyze 48 patients with autoimmune FXIII deficiency and 4 additional FXIII deficient patients who developed anti-FXIII alloantibody. The patients were collected from peer-reviewed publications from which relevant data could be extracted...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29454954/preparation-and-characterization-of-c-phycocyanin-peptide-grafted-n-succinyl-chitosan-by-enzyme-method
#7
Wenyan Xu, Yao Xiao, Pengfeng Luo, Lihong Fan
C‑phycocyanin peptide (CPC) grafted N‑succinyl chitosan (NSC) was prepared via the catalysis of Microbial transglutaminase (MTGase). The single factor experiment displayed that the degree of substitution (DS) of N‑succinyl chitosan‑C‑phycocyanin peptide (NSC‑CPC) depended on the reaction time, the reaction temperature and the reaction pH value. The CS, synthesized NSC and NSC‑CPC were characterized by Fourier transform infrared spectroscopy (FT-IR). NSC‑CPC showed excellent moisture absorption and retention ability...
February 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29449533/non-alcoholic-fatty-liver-disease-severity-is-modulated-by-transglutaminase-type-2
#8
Mauro Piacentini, Andrea Baiocchini, Franca Del Nonno, Gerry Melino, Nickolai A Barlev, Federica Rossin, Manuela D'Eletto, Laura Falasca
Non-alcoholic fatty liver disease (NAFLD) is one of the most important liver diseases worldwide. Currently, no effective treatment is available, and NAFLD pathogenesis is incompletely understood. Transglutaminase type 2 (TG2) is a ubiquitous enzyme whose dysregulation is implicated in the pathogenesis of various human diseases. Here we examined the impact of TG2 on NAFLD progression using the high-fat-diet-induced model in both wild-type and TG2-deficient mice. Animals were fed with a standard chow diet or a high-fat diet (42% of the energy from fat) for 16 weeks...
February 15, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29444780/the-possible-link-between-coeliac-and-kawasaki-diseases-in-brazil-a-cross-sectional-study
#9
Alessandra Dos Santos Domingues, Nicole Selleski, Rosa Harumi Uenishi, Cristina Medeiros Ribeiro de Magalhães, Lenora Gandolfi, Claudia B Pratesi
BACKGROUND: Kawasaki disease (KD) is a self-limited acute systemic vasculitis of unknown aetiology that predominantly affects infants and young children eventually associated with immunological abnormalities. Coeliac disease (CD) is an inflammatory autoimmune disease characterised by a permanent gluten intolerance, which affects genetically susceptible individuals of any age group, and can cause intestinal and systemic symptoms. Association of CD with KD has been previously described in a single study that disclosed a surprisingly high prevalence of CD in children with a history of KD...
February 14, 2018: BMJ Open
https://www.readbyqxmd.com/read/29435875/a-prospective-study-to-evaluate-the-role-of-duodenal-bulb-biopsy-in-the-diagnosis-of-celiac-disease
#10
Bhanwar Singh Dhandhu, Gaurav Kumar Gupta, Shashank J Wanjari, Nidhi Sharma, Sandeep Nijhawan
BACKGROUND: Celiac disease (CeD) requires a biopsy from the small intestine to confirm the diagnosis. Conventionally, duodenal bulb (D1) was avoided as a biopsy site due to histological confounding factors at this site. However, sometimes, the bulb mucosa is the only affected site. The aim of the present study was to assess changes in duodenal bulb histology and compare it to distal duodenal histology and to analyze whether the addition of duodenal bulb biopsy increases the diagnostic yield of the CeD...
February 13, 2018: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://www.readbyqxmd.com/read/29431870/a-quarter-of-patients-with-type-1-diabetes-have-co-existing-non-islet-autoimmunity-the-findings-of-a-uk-population-based-family-study
#11
Aizhan Kozhakhmetova, Rebecca C Wyatt, Claire Caygill, Claire Williams, Anna E Long, Kyla Chandler, Rachel J Aitken, Janet M Wenzlau, Howard W Davidson, Kathleen M Gillespie, Alistair J K Williams
Individuals with type 1 diabetes (T1D) are at increased risk of coeliac disease (CD), autoimmune thyroiditis and autoimmune gastritis, but the absolute risks are unclear. The aim of this study was to investigate the prevalence of autoantibodies to tissue transglutaminase (TGA), thyroid peroxidase (TPOA), and gastric H+ /K+ -ATPase (ATPA) and their genetic associations in a well-characterised population-based cohort of individuals with T1D from the Bart's-Oxford family study for whom islet autoantibody prevalence data were already available...
February 12, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29430769/illuminating-structure-and-acyl-donor-sites-of-a-physiological-transglutaminase-substrate-from-streptomyces-mobaraensis
#12
Norbert E Juettner, Stefan Schmelz, Jan P Bogen, Dominic Happel, Wolf-Dieter Fessner, Felicitas Pfeifer, Hans-Lothar Fuchsbauer, Andrea Scrima
Transglutaminase from Streptomyces mobaraensis (MTG) has become a powerful tool to covalently and highly specifically link functional amines to glutamine donor sites of therapeutic proteins. However, details regarding the mechanism of substrate recognition and interaction of the enzyme with proteinaceous substrates still remain mostly elusive. We have determined the crystal structure of the Streptomyces papain inhibitory protein (SPIp ), a substrate of MTG, to study the influence of various substrate amino acids on positioning glutamine to the active site of MTG...
February 12, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29424456/characterization-of-paroxysmal-gluten-sensitive-dyskinesia-in-border-terriers-using-serological-markers
#13
M Lowrie, O A Garden, M Hadjivassiliou, D S Sanders, R Powell, L Garosi
BACKGROUND: Paroxysmal gluten-sensitive dyskinesia (PGSD) in border terriers (BTs) results from an immunologic response directed against transglutaminase (TG)2 and gliadin. Recent evidence suggests that PGSD is only one aspect of a range of possible manifestations of gluten sensitivity in the breed. HYPOTHESIS/OBJECTIVES: Gluten sensitivity in BTs is a heterogeneous disease process with a diverse clinical spectrum; to characterize the phenotype of PGSD using TG2 and gliadin autoantibodies as diagnostic markers...
February 9, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29422338/linear-%C3%AE-1%C3%A2-%C3%A2-%C3%A2-6-d-glucan-from-bifidobacterium-bifidum-bim-%C3%B0-733d-is-low-molecular-mass-biopolymer-with-unique-immunochemical-properties
#14
Elena P Kiseleva, Konstantin I Mikhailopulo, Evelina L Zdorovenko, Yuriy A Knirel, Galina I Novik
Role of microorganisms in induction of/protection from autoimmune diseases is proven though molecular mechanisms and bacterial/viral/yeast biopolymers responsible for these effects are in the research stage. Autoantobodies (AAbs) to thyroid peroxidase (anti-TPO) and thyroglobulin (anti-Tg) as well as AAbs to transglutaminase 2 (anti-TG2) and antibodies to gliadins (anti-gliadins) are serological markers of autoimmune thyroid disease and celiac disease, respectively, and players in pathogenesis of these autoimmune diseases...
December 20, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29417981/immunoexpression-of-iga-receptors-cd89-cd71-in-dermatitis-herpetiformis
#15
Justyna Gornowicz-Porowska, Agnieszka Seraszek-Jaros, Monika Bowszyc-Dmochowska, Elżbieta Kaczmarek, Marian Dmochowski
INTRODUCTION: The role of IgA receptors in dermatitis herpetiformis (DH) pathogenesis is still unknown. CD89 and CD71 may be associated with immune response during DH development. The purpose of this study was to perform semiquantitative analysis of simultaneous immunoexpression of CD89 and CD71 in DH and IgA/neutrophil-mediated non-DH dermatoses (IgAN) in relation to specific IgA autoantibodies/antibodies (tissue and epidermal transglutaminases, nonapeptides of gliadin - eTG/tTG/npG) as well neutrophil activation via the release of neutrophil elastase (NE)...
February 8, 2018: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/29393747/practical-approaches-for-overcoming-challenges-in-heightened-characterization-of-antibody-drug-conjugates-with-new-methodologies-and-ultrahigh-resolution-mass-spectrometry
#16
Olga V Friese, Jacquelynn N Smith, Paul W Brown, Jason C Rouse
Antibody-drug conjugation strategies are continuously evolving as researchers work to improve the safety and efficacy of the molecules. However, as a part of process and product development, confirmation of the resulting innovative structures requires new, specialized mass spectrometry (MS) approaches and methods, as compared to those already established for antibody-drug conjugates (ADCs) and the heightened characterization practices used for monoclonal antibodies (mAbs), in order to accurately elucidate the resulting conjugate forms, which can sometimes have labile chemical bonds and more extreme chemical properties like hydrophobic patches...
February 2, 2018: MAbs
https://www.readbyqxmd.com/read/29392873/gluten-free-diet-exclusively-during-pregnancy-alleviates-the-signs-of-diabetes-and-celiac-disease-in-nod-mouse-offspring
#17
Martin Haupt-Jorgensen, Jesper Larsen, Knud Josefsen, Tina Z Jørgensen, Julie Christine Antvorskov, Axel K Hansen, Karsten Buschard
BACKGROUND: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in non-obese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero. METHODS: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition...
February 2, 2018: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/29386353/the-candida-albicans-eno1-gene-encodes-a-transglutaminase-involved-in-growth-cell-division-morphogenesis-and-osmotic-protection
#18
Elizabeth Reyna-Beltrán, María Iranzo, Karla Grisel Calderón-González, Ricardo Móndragon-Flores, María Luisa Labra-Barrios, Salvador Mormeneo, Juan Pedro Luna-Arias
Candida albicans is an opportunistic fungus that is part of the normal microflora commonly found in the human digestive tract and the normal mucosa or skin of healthy individuals. However, in immunocompromised individuals, it becomes a serious health concern and a threat to their lives, and is ranked as the leading fungal infection in humans worldwide. As existing treatments for this infection are non-specific or under threat of developing resistance, there is a dire necessity to find new targets for designing specific drugs to defeat this fungus...
January 31, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29383769/serum-intestinal-fatty-acid-binding-protein-in-the-noninvasive-diagnosis-of-celiac-disease
#19
Irene B Oldenburger, Victorien M Wolters, Tineke Kardol-Hoefnagel, Roderick H J Houwen, Henny G Otten
Current diagnostic guidelines for celiac disease (CD) in pediatric patients require a duodenal biopsy if the IgA anti-tissue transglutaminase (tTG) is below 10x the upper limit of normal (ULN). Additional markers may enable a noninvasive diagnosis in this group. Serum intestinal-fatty acid-binding protein (I-FABP), a marker for intestinal epithelial damage, could be useful in this respect. A total of 95 children with a clinical suspicion of CD and tTG 1-10x ULN were investigated. All had a duodenal biopsy and analysis of serum I-FABP...
January 31, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29382685/proteomic-profiling-reveals-the-transglutaminase-2-externalization-pathway-in-kidneys-after-unilateral-ureteric-obstruction
#20
Giulia Furini, Nina Schroeder, Linghong Huang, David Boocock, Alessandra Scarpellini, Clare Coveney, Elisa Tonoli, Raghavendran Ramaswamy, Graham Ball, Claudia Verderio, Timothy S Johnson, Elisabetta A M Verderio
Increased export of transglutaminase-2 (TG2) by tubular epithelial cells (TECs) into the surrounding interstitium modifies the extracellular homeostatic balance, leading to fibrotic membrane expansion. Although silencing of extracellular TG2 ameliorates progressive kidney scarring in animal models of CKD, the pathway through which TG2 is secreted from TECs and contributes to disease progression has not been elucidated. In this study, we developed a global proteomic approach to identify binding partners of TG2 responsible for TG2 externalization in kidneys subjected to unilateral ureteric obstruction (UUO) using TG2 knockout kidneys as negative controls...
January 30, 2018: Journal of the American Society of Nephrology: JASN
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