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Clara Valero, Hippolyte Amaveda, Mario Mora, Jose Manuel García-Aznar
Collagen hydrogels are widely used for in-vitro experiments and tissue engineering applications. Their use has been extended due to their biocompatibility with cells and their capacity to mimic biological tissues; nevertheless their mechanical properties are not always optimal for these purposes. Hydrogels are formed by a network of polymer filaments embedded on an aqueous substrate and their mechanical properties are mainly defined by the filament network architecture and the individual filament properties...
2018: PloS One
Robert Wodtke, Christoph Hauser, Gloria Ruiz-Gomez, Elisabeth Jäckel, David Bauer, Martin Lohse, Alan Wong, Johanna Pufe, Friedrich-Alexander Ludwig, Steffen Fischer, Sandra Hauser, Dieter Greif, Maria Teresa Pisabarro, Jens Pietzsch, Markus Pietsch, Reik Löser
Transglutaminase 2 (TGase 2)-catalysed transamidation represents an important posttranslational mechanism for protein modification with implications in physiological and pathophysiological conditions including fibrotic and neoplastic processes. Consequently, this enzyme is considered a promising target for the diagnosis and therapy of these diseases. In this study, we report on the synthesis and kinetic characterisation of Nε -acryloyllysine piperazides as irreversible inhibitors of TGase 2. Systematic structural modifications on 54 new compounds were performed with a major focus on fluorine-bearing substituents due to the potential of such compounds to serve as radiotracer candidates for positron emission tomography...
April 17, 2018: Journal of Medicinal Chemistry
Huifang Sun, Mari T Kaartinen
Osteoclasts, bone resorbing cells, derive from monocyte/macrophage cell lineage. Increased osteoclast activity is responsible for bone destruction in diseases such as osteoporosis, periodontitis and rheumatoid arthritis. Transglutaminases (TGs), protein crosslinking enzymes, were recently found involved in osteoclastogenesis in vivo, however their mechanisms of action have remained unknown. In this study, we have investigated the role of TG activity in osteoclastogenesis in vitro using four TG inhibitors, NC9, Z006, T101, and monodansyl cadaverine...
April 16, 2018: Journal of Cellular Physiology
Lucia Padalino, Isabella D'Antuono, Miriana Durante, Amalia Conte, Angela Cardinali, Vito Linsalata, Giovanni Mita, Antonio F Logrieco, Matteo Alessandro Del Nobile
BACKGROUND: During recent years food industries generally produce a large volume of wastes both solid and liquid, representing a disposal and potential environmental pollution problem. OBJECTIVE: The goal of the study was to optimize, from both sensory and nutritional points of view, the formulation of durum wheat spaghetti enriched with an olive oil industrial by-product, indicated as olive paste. METHODS: Three consecutive steps were carried out...
April 16, 2018: Antioxidants (Basel, Switzerland)
Liguo Qiu, Xiang Shi, Simeng Yu, Qian Han, Xiaoping Diao, Hailong Zhou
Ammonia stress can inhibit the survival and growth, and even cause mortality of shrimp. In this study, ammonia-metabolizing enzyme activities and gene expression were compared between two strains of L. vannamei under different ammonia-N ([Formula: see text]) concentrations (3.4, 13.8, and 24.6 mg/L). The results showed that elevated ammonia concentrations mainly increased glutamine synthetase (GSase) activities while inhibiting transglutaminase (TGase) activities in the muscle of both strains. Thus, we concluded that L...
2018: Frontiers in Physiology
Barbara Spolaore, Giacomo Forzato, Angelo Fontana
Microbial transglutaminase (TGase) has been successfully used to produce site-specific protein conjugates derivatized at the level of glutamine (Gln) or lysine (Lys) residues with diverse applications. Here, we study the drug human interferon β-1a (IFN) as a substrate of TGase. The derivatization reaction was performed using carbobenzoxy-L-glutaminyl-glycine to modify Lys residues and dansylcadaverine for Gln residues. The 166 amino acids polypeptide chain of IFN β-1a contains 11 Lys and 11 Gln residues potential sites of TGase derivatization...
April 7, 2018: Amino Acids
Takashi Kojima, Cem Simsek, Ayako Igarashi, Kazue Aoki, Kazunari Higa, Takahiko Shimizu, Murat Dogru, Kazuo Tsubota, Jun Shimazaki
Purpose: The superoxide dismutase-1 knockout (Sod1-/-) mouse is an age-related dry eye mouse model. We evaluated the role of 2% rebamipide ophthalmic solution on the conjunctiva and ocular surface alterations in Sod1-/- mice. Methods: Rebamipide eye drops (2%) were instilled in six 50-week-old male Sod1-/- mice and six C57BL/6 strain wild-type (WT) male mice four times a day for 2 weeks. Aqueous tear secretion quantity and tear film breakup time measurements as well as vital stainings were performed...
March 1, 2018: Investigative Ophthalmology & Visual Science
Eui Man Jeong, Chun Zi Jin, Ji Hyun Jang, Zai Hao Zhao, Chun Li Jin, Jin Hang Lee, Ki Baek Lee, Sung Joon Kim, In-Gyu Kim, Yin Hua Zhang
The myocardium in hypertensive heart exhibits decreased fatty acid utilization and contractile dysfunction, leading to cardiac failure. However, the causal relationship between metabolic remodeling and cardiomyocyte contractility remains unestablished. Transglutaminase 2 (TG2) has been known to promote ATP production through the regulation of mitochondrial function. In this study, we investigated the involvement of TG2 in cardiomyocyte contraction under fatty acid supplementation. Using TG2 inhibitor and TG2-deficient mice, we demonstrated that fatty acid supplementation activated TG2 and increased ATP level and contractility of cardiac myocyte from the normal heart...
April 6, 2018: Experimental & Molecular Medicine
Yueqi An, Juan You, Shanbai Xiong, Tao Yin
To enhance the application of transglutaminase (TGase) in processing and preserving of frozen surimi, the mechanism that enhances surimi gelation induced by TGase after short-term freezing was investigated. Gel properties and cross-linking extent of surimi gels increased when surimi was frozen at -18 °C for 5-7 days. However when storage time exceeded 10 days, textural properties and water-holding capacity decreased significantly. Moreover, the difference of breaking force and hardness between surimi gels with and without TGase reached the maximum after 3-5 days of frozen storage...
August 15, 2018: Food Chemistry
Ankit Chhoda, Deepanshu Jain, Mradul Kumar Daga, Vineeta Batra
We report a rare case of secondary renal amyloidosis in a patient with probable celiac disease presenting as nephrotic syndrome. A 30-year-old man with chronic diarrhea presented with progressive anasarca for 2 years. On further evaluation, he had hypoalbuminemia, significant nephrotic-range proteinuria, and elevated levels of anti-tissue transglutaminase. Renal biopsy suggested deposition of amorphous Congo red-positive material in the glomerular mesangium, positive for amyloid A amyloidosis. Endoscopic duodenal biopsy suggested blunting of the villous architecture and chronic inflammation of the lamina propria...
2018: ACG Case Reports Journal
William L Willis, Linan Wang, Takuma Tsuzuki Wada, Mark Gardner, Omar Abdouni, Jeffrey Hampton, Giancarlo Valiente, Nicholas Young, Stacy Ardoin, Sudha Agarwal, Michael A Freitas, Lai-Chu Wu, Wael N Jarjour
High-mobility group box 1 (HMGB1) is a chromatin-associated protein that in response to stress or injury, translocates from the nucleus to the extracellular milieu where it functions as an alarmin. HMGB1's function is in part determined by the complexes (HMGB1c) it forms with other molecules. However, structural modifications in the HMGB1 polypeptide that may regulate HMGB1c formation have not been previously described. In this report, we observed high-molecular weight, denaturing-resistant HMGB1c in the plasma and peripheral blood mononuclear cells of individuals with systemic lupus erythematosus (SLE) and to a much lesser extent in healthy subjects...
April 4, 2018: Journal of Biological Chemistry
Amelia Chen, Indra Tanidjaja, Srinivasan Damodaran
Whey proteins (WPI) were polymerized with transglutaminase (TGase) before and after partially hydrolyzing the protein with thermolysin to produce protein nanoparticles/polymers. Electrophoresis and atomic force microscopy (AFM) were used to determine the size and structural characteristics of the polymers. The foaming and emulsifying properties of these nanoparticles were studied. The polymerized WPI (WPI-TG) produced more stable foams than the repolymerized WPI hydrolysate (WPIH-TG). In contrast, WPIH-TG produced better emulsions with better storage stability than WPI-TG emulsions...
August 1, 2018: Food Chemistry
D Santi Swarupini, Abani K Bhuyan
Arabidopsis thaliana Phloem Protein 16-1 (AtPP16-1) is a 156-residue intrinsically disordered nucleic acid binding protein which is putatively involved in long-distance systemic transport of RNA to budding regions in plants. Dimerization or oligomerization of the protein at pH higher than about 4.1 leaves no apolar surface exposed for interaction with the dye 8-Anilinonaphthalene-1-sulfonate (ANS). The most stable monomeric state is found near pH 4 where the structure of the protein is determined to have three short β-strands and a single α-helix...
March 26, 2018: Biophysical Chemistry
Asad Abbas, Tabassum Shahab, Rana K Sherwani, Seema Alam
Background A gluten-free diet (GFD) is the standard of care in the management of patients with celiac disease, but clinical and histological recovery are often delayed. In newly diagnosed patients, strict compliance to GFD is difficult to achieve; this is especially true in developing countries where gluten-free food is often difficult to obtain. Steroids, when used alone, can be effective in inducing recovery in patients with celiac disease. We performed a randomized controlled trial to study the effect of a short course of prednisolone combined with a GFD on the recovery of celiac disease...
January 28, 2018: Curēus
Michael J Lentze, Salvatore Auricchio, Samy Cadranel, Peter J Milla, Deirdre Kelly, Alexander S McNeish, Jean Rey, Jacques Schmitz, Birgitta Strandvik, Jan Taminiau, Jarmo Visakorpi
Thirty-six founding members from Europe were present in 1968, when the European Society of Paediatric Gastroenterology (ESGA) had its first meeting in Paris. The aim was to create a forum for presentations and discussions of research activities in paediatric gastroenterology in Europe. At the second meeting of ESGA 1969 in Interlaken, an important landmark was set for all gastroenterologists in the world. In this conference, the first ever criteria for "the Diagnosis of Coeliac Disease" (CD) were established...
April 2018: Journal of Pediatric Gastroenterology and Nutrition
Ajay Tijore, Jean-Marc Behr, Scott Alexander Irvine, Vrushali Baisane, Subbu Venkatraman
Three dimensional (3D) bioprinting has been proposed as a method for fabricating tissue engineered small diameter vascular prostheses. This technique not only involves constructing the structural features to obtain a desired pattern but the morphology of the pattern may also be used to influence the behavior of seeded cells. Herein, we 3D bioprinted a gelatin hydrogel microchannel construct to promote and preserve the contractile phenotype of vascular smooth muscle cells (vSMCs), which is crucial for vasoresponsiveness...
March 28, 2018: Biomedical Microdevices
Paulo C Maffía, Diego Guerrieri, Ximena Villalonga, Fiorella Caro, Sonia Gómez, Nancy Tateosian, Betiana P Bogado, Mercedes L Sánchez, Nella Ambrosi, Eduardo Chuluyan
Secretory Leukocyte Proteinase Inhibitor (SLPI) is an antiinflammatory peptide that blocks the activity of serine proteases, primarily the neutrophil elastase. In an attempt to direct the activity of SLPI on inflamed sites, a chimera consisting of the transglutaminase II substrate domain of trappin 2 (cementoin), and the mature SLPI protein was constructed. Cell attachment and biological activity were compared between SLPI and this chimera. By using whole cell ELISA, fluorescence microscopy and flow cytometry assays we observed that the cementoin-SLPI fusion protein (FP) but not SLPI attached to a human lung epithelial cell line and monocytes...
March 28, 2018: Scientific Reports
Elham Mohammad Zadeh, Sean F O'Keefe, Young-Teck Kim, Jin-Hun Cho
The effects of transglutaminase on soy protein isolate (SPI) film forming solution and films were investigated by rheological behavior and physicochemical properties based on different manufacturing conditions (enzyme treatments, enzyme incubation times, and protein denaturation temperatures). Enzymatic crosslinking reaction and changes in molecular weight distribution were confirmed by viscosity measurement and SDS-PAGE, respectively, compared to 2 controls: the nonenzyme treated and the deactivated enzyme treated...
March 25, 2018: Journal of Food Science
Michael J Selig, B N Dar, Arkaye Kierulf, Raheleh Ravanfar, Syed S H Rizvi, Alireza Abbaspourrad
Treatment of whey protein isolate (WPI; 1 to 25% w/w) in heated κ-carrageenan (KC; 2% w/w) slurries with protease and/or transglutaminase modulated the properties of the hydrogels formed after cooling. Observation of peak compression stress and strain at gel rupture showed WPI incorporation at 1, 5 and 10% (w/w) significantly reduced the strength and deformability of 2% (w/w) KC gels. Treatment of WPI solutions in KC slurries with Alcalase 2.4L was shown by both SDS-Page and size exclusion HPLC to reduce protein/peptide molecular weight distributions below 10 kDa, with large portions below 1 kDa...
March 26, 2018: Food & Function
Ted B Taylor, John J Zone
No abstract text is available yet for this article.
March 20, 2018: Journal of Investigative Dermatology
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