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transglutaminase

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https://www.readbyqxmd.com/read/29222550/tissue-transglutaminase-elevates-intraocular-pressure-in-mice
#1
Urmimala Raychaudhuri, J Cameron Millar, Abbot F Clark
Purpose: Tissue transglutaminase (TGM2) is elevated in glaucomatous trabecular meshwork (TM) tissues. We investigated whether increased expression of TGM2 increases extracellular matrix crosslinking in the TM, thereby increasing aqueous humor outflow resistance and elevating intraocular pressure (IOP) in mouse eyes. Methods: GTM3, primary human GTM 125-05, and cultured mouse TM cells were transduced with adenovirus serotype 5 expressing human transglutaminase 2 (Ad5...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29210921/celiac-disease-by-the-age-of-13-years-is-not-associated-with-probiotics-administration-in-infancy
#2
Emma Merike Savilahti, Jorma Ilonen, Anna Kaarina Kukkonen, Erkki Savilahti, Mikael Kuitunen
Probiotics are theoretically promising in primary prevention of celiac disease (CD), but research evidence on the topic is scarce. We used the data and material of a clinical double-blind randomized placebo-controlled trial on primary allergy prevention (n = 1223) to investigate in an exploratory study whether administration of a mix of pro- and prebiotics during late pregnancy and first 6 months of life was associated with prevalence of CD during 13-year follow-up. Children who fulfilled diagnostic criteria for CD (n = 11) and subjects with a serum sample available for analyzing CD-antibodies (n = 867) were included...
November 28, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29208472/role-of-transglutaminase-2-in-a1-adenosine-receptor-and-%C3%AE-2-adrenoceptor-mediated-pharmacological-pre-and-post-conditioning-against-hypoxia-reoxygenation-induced-cell-death-in-h9c2-cells
#3
Falguni S Vyas, Carl P Nelson, John M Dickenson
Pharmacologically-induced pre- and post-conditioning represent attractive therapeutic strategies to reduce ischaemia/reperfusion injury during cardiac surgery and following myocardial infarction. We have previously reported that transglutaminase 2 (TG2) activity is modulated by the A1 adenosine receptor and β2-adrenoceptor in H9c2 cardiomyoblasts. The primary aim of this study was to determine the role of TG2 in A1 adenosine receptor and β2-adrenoceptor-induced pharmacological pre- and post-conditioning in the H9c2 cells...
December 2, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29205566/transglutaminase-type-2-plays-a-key-role-in-the-pathogenesis-of-mycobacterium-tuberculosis-infection
#4
I Palucci, I Matic, L Falasca, M Minerva, G Maulucci, M De Spirito, E Petruccioli, D Goletti, F Rossin, M Piacentini, G Delogu
BACKGROUND: Mycobacterium tuberculosis (MTB), the aetiological agent of tuberculosis (TB), is capable of interfering with the phagosome maturation pathway, by inhibiting phagosome-lysosome fusion and the autophagic process to ensure survival and replication in macrophages. Thus, it has been proposed that the modulation of autophagy may represent a therapeutic approach to reduce MTB viability by enhancing its clearance. OBJECTIVE: The aim of this study was to investigate whether transglutaminase type 2 (TG2) is involved in the pathogenesis of MTB...
December 4, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/29204051/intestinal-epithelium-intraepithelial-lymphocytes-and-the-gut-microbiota-key-players-in-the-pathogenesis-of-celiac-disease
#5
REVIEW
Bożena Cukrowska, Agnieszka Sowińska, Joanna Beata Bierła, Elżbieta Czarnowska, Anna Rybak, Urszula Grzybowska-Chlebowczyk
Celiac disease (CD) is a chronic immune-mediated disorder triggered by the ingestion of gluten in genetically predisposed individuals. Before activating the immune system, gluten peptides are transferred by the epithelial barrier to the mucosal lamina propria, where they are deamidated by intestinal tissue transglutaminase 2. As a result, they strongly bind to human leucocyte antigens (HLAs), especially HLA-DQ2 and HLA-DQ8, expressed on antigen-presenting cells. This induces an inflammatory response, which results in small bowel enteropathy...
November 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29198166/improvement-of-the-activity-and-thermostability-of-microbial-transglutaminase-by-multiple-site-mutagenesis
#6
Dongdong Mu, Jiaojiao Lu, Chang Shu, Haowen Li, Xingjiang Li, Jing Cai, Shuizhong Luo, Peizhou Yang, Shaotong Jiang, Zhi Zheng
Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase was performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min).
December 4, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29197584/depletion-of-transglutaminase-2-in-neurons-alters-expression-of-extracellular-matrix-and-signal-transduction-genes-and-compromises-cell-viability
#7
Laura Yunes-Medina, Alex Paciorkowski, Yan Nuzbrokh, Gail V W Johnson
The protein transglutaminase 2 (TG2) has been implicated as a modulator of neuronal viability. TG2's role in mediating cell survival processes has been suggested to involve its ability to alter transcriptional events. The goal of this study was to examine the role of TG2 in neuronal survival and to begin to delineate the pathways it regulates. We show that depletion of TG2 significantly compromises the viability of neurons in the absence of any stressors. RNA sequencing revealed that depletion of TG2 dysregulated the expression of 86 genes with 59 of these being upregulated...
November 29, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29187283/new-insights-into-transglutaminase-2-and-links-to-neurodegenerative-diseases
#8
Boram Min, Kwang Chul Chung
Formation of toxic protein aggregates is a common feature and mainly contributes to the pathogenesis of neurodegenerative diseases (NDDs), which include amyotrophic lateral sclerosis (ALS), Alzheimer's, Parkinson's, Huntington's, and prion diseases. The transglutaminase 2 (TG2) gene encodes a multifunctional enzyme, displaying four types of activity, such as transamidation, GTPase, protein disulfide isomerase, and protein kinase activities. Many studies demonstrated that the calcium-dependent transamidation activity of TG2 affected the formation of insoluble and toxic amyloid aggregates that mainly consisted of NDD-related proteins...
November 29, 2017: BMB Reports
https://www.readbyqxmd.com/read/29182792/ex-vivo-culture-of-duodenal-biopsies-from-patients-with-dermatitis-herpetiformis-indicates-that-transglutaminase-3-antibody-production-occurs-in-the-gut
#9
Minna Hietikko, Kaisa Hervonen, Tuire Ilus, Teea Salmi, Heini Huhtala, Kaija Laurila, Tiina Rauhavirta, Timo Reunala, Katri Kaukinen, Katri Lindfors
Coeliac disease and dermatitis herpetiformis (DH) are characterized by autoantibodies targeting transglutaminase (TG)2 and TG3, respectively. Previous studies show that TG2 antibodies are produced in the gut and can be assessed in organ culture of small-intestinal biopsies from patients with coeliac disease. Thus far, no studies have investigated TG3 antibodies in organ culture of biopsies from patients with DH, or exploited the method in DH. The aim of this study was to investigate TG3 and TG2 antibody responses in serum and small-intestinal biopsies from patients with DH with active disease, and from those in remission...
November 28, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/29179065/reaction-profiling-of-a-set-of-acrylamide-based-human-tissue-transglutaminase-inhibitors
#10
Mahmood H Jasim, Daniel L Rathbone
The major function of the enzyme human tissue transglutaminase (TG2) is the crosslinking of proteins via a transamidation between the γ-carboxamide of a glutamine and the ε-amino group of a lysine. Overexpression of TG2 can lead to undesirable outcomes and has been linked to conditions such as fibrosis, celiac disease and neurodegenerative diseases. Accordingly, TG2 is a tempting drug target. The most effective TG2 inhibitors to date are small-molecule peptidomimetics featuring electrophilic warheads that irreversibly modify the active site catalytic cysteine (CYS277)...
November 22, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29178246/effects-of-the-antagomirs-15b-and-200b-on-the-altered-healing-pattern-of-diabetic-mice
#11
Gabriele Pizzino, Natasha Irrera, Federica Galfo, Giovanni Pallio, Federica Mannino, Angelica D'amore, Enrica Pellegrino, Antonio Ieni, Giuseppina T Russo, Marco Calapai, Domenica Altavilla, Francesco Squadrito, Alessandra Bitto
BACKGROUND AND PURPOSE: Diabetic subjects with non-healing ulcers have a reduced expression of vascular endothelial growth factor (VEGF). Genetically diabetic mice have an altered expression pattern of VEGF and its receptor, VEGFR-2. In diabetic wounds, miR15b and miR200b which respectively inhibit VEGF and VEGF-R2 mRNAs are up-regulated, further affecting the impaired angiogenesis. We investigated whether anti-miRs directed toward miR15b and miR200b could improve wound repair in genetically diabetic mice...
November 26, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29175523/improved-mechanical-properties-and-thermal-stability-of-collagen-fiber-based-film-bycrosslinking-with-casein-keratin-or-spi-effect-of-crosslinking-process-and-concentrations-of-proteins
#12
Xiaomeng Wu, Anjun Liu, Wenhang Wang, Ran Ye
This study utilized three different thermo-stable proteins of casein, keratin and soy protein isolate (SPI) to improve the thermal stabilities and mechanical properties of collagen fiber films using transglutaminase (TGase) crosslinking. The crosslinking greatly enhanced the thermal- stability of collagen fiber films, especially that of the collagen fiber crosslinking with 50% casein composite films, judged from thermogravimetric analysis (TGA). Furthermore, the TGase treatment improved the mechanical properties of the collagen fiber films interms of tensile strength (TS) and elongation at break (EAB)...
November 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29170410/endothelial-cell-surface-tissue-transglutaminase-inhibits-neutrophil-adhesion-by-binding-and-releasing-nitric-oxide
#13
Thung-S Lai, Robert A Lindberg, Hua-Lin Zhou, Zishan A Haroon, Mark W Dewhirst, Alfred Hausladen, Y-L Juang, Jonathan S Stamler, Charles S Greenberg
Nitric oxide (NO) produced by endothelial cells in response to cytokines displays anti-inflammatory activity by preventing the adherence, migration and activation of neutrophils. The molecular mechanism by which NO operates at the blood-endothelium interface to exert anti-inflammatory properties is largely unknown. Here we show that on endothelial surfaces, NO is associated with the sulfhydryl-rich protein tissue transglutaminase (TG2), thereby endowing the membrane surfaces with anti-inflammatory properties...
November 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29169946/preparation-and-characterization-of-chitosan-collagen-peptide-oxidized-konjac-glucomannan-hydrogel
#14
Liangling Liu, Huigao Wen, Ziqie Rao, Chen Zhu, Meng Liu, Lian Min, Lihong Fan, Shengxiang Tao
In this paper, the microbial transglutaminase (MTGase) was used as a catalyst to graft the collagen peptide (COP) molecules on the amino group of chitosan to obtain water-soluble chitosan-collagen peptide (CS-COP) derivatives. The preparation of composite hydrogel was via the Schiff-base reaction between the amino of CS-COP and the aldehyde of oxidized konjac glucomannan (OKGM). The hydrogels were characterized by various techniques including Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM)...
November 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29168862/inhibition-of-advanced-glycation-endproducts-during-fish-sausage-preparation-by-transglutaminase-and-chitosan-oligosaccharides-induced-enzymatic-glycosylation
#15
Jing Wang, Long Zou, Fangzhou Yuan, Liangtao Lv, Shenglan Tian, Zhenxing Li, Hong Lin
Advanced glycation endproducts (AGEs) are the harmful products of non-enzymatic reactions in foods formed during the heating process. In order to reduce the content of AGEs in foods, the inhibitory effect of different proportions of transglutaminase (TGase) and chitosan oligosaccharides (COS) on the AGEs of seabream fish meat sausage was studied. The results indicated that the TGase/COS ratio of 1 : 1 could inhibit the formation of AGEs, showing especially a decreased N(ε)-carboxymethyl-lysine (CML) content and a twice higher inhibition rate (36...
November 23, 2017: Food & Function
https://www.readbyqxmd.com/read/29168584/hemolysis-and-iga-antibodies-against-tissue-transglutaminase-when-are-antibody-test-results-no-longer-reliable
#16
Johannes Wolf, Norman Haendel, Johannes Remmler, Carl Elias Kutzner, Thorsten Kaiser, Thomas Mothes
BACKGROUND: Antibodies against tissue transglutaminase (TTG) of isotype IgA (IgA-aTTG) represent reliable diagnostic markers to confirm or exclude celiac disease (CD). Hemolysis (HL) is an important pre-analytical factor. HL can be quantified as HL index (HI) correlating with the concentration of free hemoglobin. TTG is abundant in erythrocytes and released upon HL. In immunoassays, the released TTG may interfere with binding of IgA-aTTG to the coated TTG. METHODS: We selected 17 HL-free sera from children with biopsy-confirmed CD: 7 with low-positive (1-5 multiples of upper limit of normal [×ULN]), 5 with intermediate (5-10 × ULN) and 5 with high IgA-aTTG (10-15 × ULN)...
November 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29162372/genetics-environment-and-asthma-associated-with-celiac-disease-in-the-extended-family-of-an-affected-child
#17
R Sigala-Robles, S V Aguayo-Patrón, A M Calderón de la Barca
INTRODUCTION: Celiac disease (CD) is an autoimmune enteropathy associated with gluten ingestion. In extended families of celiac patients that live in close proximity of one another, shared genetic and environmental factors can predispose them to CD. AIM: The aim of this study was to provide evidence about the genetic and environmental factors involved in the development of CD in the extended family of a pediatric patient. METHODS: The medical history, environmental conditions, and participant weight, height, and peripheral blood samples were evaluated...
November 18, 2017: Revista de Gastroenterología de México
https://www.readbyqxmd.com/read/29147086/edible-packaging-film-derived-from-mechanically-deboned-chicken-meat-proteins-effect-of-transglutaminase-on-physicochemical-properties
#18
Damla Yayli, Sadettin Turhan, Furkan Turker Saricaoglu
In this study, effect of transglutaminase (TGase) addition on physical, water barrier, optical and mechanical properties of mechanically deboned chicken meat protein (MDCM-P) films was investigated. When TGase was added to the films, the thickness increased, but the solubility decreased. Films treated with TGase exhibited higher water vapor permeability than control film (p<0.05). When TGase concentration increased, the L* values of films decreased, but a* and b* values increased. All films showed very good barrier properties against UV light...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29135820/avoiding-small-intestinal-biopsies-for-diagnosis-of-celiac-disease-in-children-a-reliable-strategy-for-all-patients
#19
Magdalena Araya, Julia Díaz, Amaya Oyarzun, Yalda Lucero, Teresa Alarcon, Mónica González, Paulina Canales, Liliana Fierro, Francisco Pérez-Bravo
BACKGROUND: Current reports applying ESPGHAN exception criteria (EEC) to diagnose celiac disease (CD) without duodenal biopsies indicate that a high percentage of patients with CD may be identified when applied correctly in specialized settings. However, application of the EEC in "daily life conditions" at the different levels of medical services is not clear. METHODS: EEC was applied to 130 pediatric patients evaluated for CD at five public hospitals in Santiago, Chile, during 2010-2015...
November 10, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29132967/unexplained-infertility-and-undiagnosed-celiac-disease-study-of-a-multiethnic-canadian-population
#20
Beth Gunn, Kellie E Murphy, Ellen M Greenblatt
OBJECTIVE: The aims of this study were to examine the prevalence of Celiac disease (CD) in Canadian women with unexplained infertility versus women with an identifiable cause of infertility and to assess the sensitivity of the point-of-care Biocard Celiac Test Kit versus standard serum serologic testing. METHODS: In this prospective cohort study, women aged 18 to 44 who were evaluated for infertility between February 2010 and May 2012 at a tertiary academic care fertility clinic in Toronto, ON, were invited to participate...
November 10, 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
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