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Drug resisitance

Yu-Kai Guo, Min Shi, Yong-Liang Qin, Yong-Jun Li
OBJECTIVE: To explore the mechanism of apoptosis for resvertrol-mediated reversing the drug-resistance of AML HL-60/ADR cells. METHODS: The HL-60/ADR cells were divided into 4 groups: control group, adriamycin (ADR)-treated group, resveratrol(Res)-treated group and ADR+Res-treated group. The inhibitory rate of cell proliferation was analyzed by CCK-8 method. The auto-fluorescence intensity of intracellular ADR and the apoptotic rate of HL-60/ADR cells were detected by flow cytometry...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
Lifen Zhao, Yujia Shan, Bing Liu, Yang Li, Li Jia
Chronic myeloid leukemia (CML) is caused by the constitutively active BCR-ABL tyrosine kinase. Although great progress has been made for improvement in clinical treatment during the past decades, it is common for patients to develop chemotherapy resistance. Therefore, further exploring novel therapeutic strategies are still crucial for improving disease outcome. MicroRNAs (miRNAs) represent a novel class of genes that function as negative regulators of gene expression. Recently, miRNAs have been implicated in several cancers...
May 25, 2017: Cell Death & Disease
Lijun Qin, Hongbing Qiu, Minjie Zhang, Fenghua Zhang, Hongfang Yang, Liu Yang, Li Jia, Kaiyun Qin, Ling Jia, Xiaomeng Dou, Lili Cheng, Meixiang Sang, Chao Zhang, Baoen Shan, Zhengmao Zhang
OBJECTIVE: CD154 (CD40L) is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Being an activator of immune cells, CD40L has also been shown to directly induce apoptosis in tumor cells by multiple mechanisms. To understand the role of sCD40L in regulating the proliferation of epithelial ovarian cancer cells treated or untreated with cisplatin...
April 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hee-Won Moon, Mina Hur, Ji-Young Kim, Yeo-Min Yun
BACKGROUND: Rifampin (RIF) is the most important first-line antituberculosis drug, and resistance to this drug may result in treatment failures. We evaluated the diagnostic performances of recently introduced, molecular assays for the detection of RIF resistance. METHODS: A total of 100 isolates (50 RIF resistant and 50 RIF susceptible) were studied. Their RIF resistances were determined by conventional drug-susceptibility test. These results were compared with those of three molecular assays: Xpert MTB/RIF assay (MTB is Mycobacterium tuberculosis), Sacace MTB Real-TM resistance, and AdvanSure MDR-TB GenoBlot assay (MDR is multidrug resisitant)...
March 2015: Journal of Clinical Laboratory Analysis
Yi Shen, Yan Chu, Yan Yang, Zehua Wang
The current view of multidrug resisitance is that overexpression of membrane P-glycoprotein (P-gp) is a major causative factor. However, the controversial presence of subcellular P-gp may also participate in the drug resistance. In this study, we sought to investigate the localization and functional characterization of P-gp in mitochondria isolated from MCF-7 and doxorubicin-resistant MCF-7 (MCF-7/ADM) cells. Mitochondria were isolated and purified from the MCF-7 cell line and its resistant cells MCF-7/ADM...
May 2012: Oncology Reports
Fenggang Xiang, Wenjuan Yu, Yi Shen, Cuijiao Wu, Yuewei Wang
BACKGROUND: Neoadjuvant chemotherapy (NACT) plays an important role in systemic chemotherapy for non-small cell lung cancer (NSCLC). P-glycoprotein (P-gp), lung resistance related protein (LRP), multidrug resistance-associated protein (MRP) and glutathione S-transferase (GST-π) may be associated to drug resisitance to chemotherapy in NSCLC. The aim of this study is to investigate the expressions of P-gp, LRP, MRP and GST-π in samples from NSCLC patients before and after treatment with NACT, and their quantitative changes, so that to evaluate the influence of NACT on drug resistance to chemotherapy of NSCLC...
October 20, 2007: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Yuxia Guo, Gaihuan Zheng, Xianqing Jin, Youhua Xu, Qing Luo, Xiaomei Liu, Zhenzhen Zhao, Yong Chen
OBJECTIVE: A novel multi-drug resistance gene named as HA117 has been screened and cloned in multidrug resisitant leukemia cell lines in our previous research, but its function is still unknown. In this study, HA117 gene was investigated whether it could increase the drug resistance in chronic myelogenous myeloid leukemia cell line K562. METHODS: HA117 was cloned and adenovirus vectors were constructed with the HA117 gene (Adeasy-HA117). K562 cells were infected by Ad-HA117 to get the K562/Ad-HA117 cells with HA117 gene expression...
2009: Journal of Experimental & Clinical Cancer Research: CR
Xi Cheng, Wenxiang Jia, Baozhong Du, Wei Zeng, Mei Kang, Zairong Zhang, Yi Xie, Weiqing Yang, Hong Fan, Chuanmin Tao, Lei Zhang
A total of 50 clinical imipenem-resistant isolates of Pseudomonas aeruginosa and Acinetobacter baumannii were subjected to the ceftazidime-2- mercaptoethanol -double-disk synergy test and to the PCR assays with primers specific for bla(IMP-1). After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii...
August 2007: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
Rong Ma, Xi-Hai Chen, Qi-Fan Zhang, Lei Zhu, Li-Ping Tang, Jing Wang, Hai-Yan Cheng, Li-Na Lei
OBJECTIVE: To explore the effects of survivin antisense RNA on apoptosis and chemosensitivity to docetaxel in gastric cancer line SGC7901 cells, and its relation to mdr-1. METHODS: survivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cells by electorporation and positive clone was screened out. survivin protein and mdr-1 mRNA were determined by Western blot and RT-PCR. Apoptosis-inducing effect was examined by electron microscopy. Sensitivity to docetaxel was examined by MTT...
February 2007: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
S I Fenik, V G Solodushko, T B Kaliniak, Ia B Blium
Nicotiana plumbaginifolia callus lines with the equal resistance to cadmium have been produced under different selective conditions--either without inhibition of the phytochelatin synthesis (line Cd-R) or in the presence of the inhibitor butionine sulfoximine (line Cd-Ri). The level of phytochelatin synthesis in the line Cd-R five-fold exceeded the control value and in the line Cd-Ri it was twice as much as in the control. It was shown that in the control line mainly three cadmium-binding proteins are expressed of the molecular weihgts 41, 34 and 19 kD...
January 2007: T︠S︡itologii︠a︡ i Genetika
M Rahbar, R Gra-Agaji, S Hashemi
In a 2-year retrospective study, the database of the microbiology laboratory of the Imam Khomeini Hospital was reviewed to identify patients who had nosocomial bacteraemia between 1 May 1999 and 31 May 2001 and identify the pathogen responsible and its resisitance to antibiotics. Of 6492 patients in various wards, 593 (9.1%) had positive blood cultures; 85 of those (14.3%) had signs of potential skin contamination. Gram-positive cocci, including coagulase-negative staphylococci, Staphylococcus aureus, Streptococcus pneumoniae and other Gram-positive cocci, accounted for 42...
May 2005: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
Mei Zeng, Quan Lu
No abstract text is available yet for this article.
August 2005: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Kan Yonemori, Noriyuki Katsumata, Hajime Uno, Koji Matsumoto, Tsutomu Kouno, Shinya Tokunaga, Yasuhiro Yamanaka, Chikako Shimizu, Masashi Ando, Masahiro Takeuchi, Yasuhiro Fujiwara
BACKGROUND: Partial cross-resistance to paclitaxel and docetaxel has been demonstrated in pre-clinical studies. PATIENTS AND METHODS: We retrospectively evaluated the efficacy of weekly paclitaxel 80 mg/m(2) in 82 patients with docetaxel-resisitant metastatic breast cancer. Docetaxel resistance was classified into primary resistance, defined as progressive disease while receiving docetaxel, and secondary resistance, defined as progression after achievement of a documented clinical response to docetaxel...
February 2005: Breast Cancer Research and Treatment
B Lü, Z Fu, S Xu
OBJECTIVE: To set up a fast, sensitive and specific way to detect mycobacteria and rifampicin susceptibility to mycobacteria by using recombinant mycobacteriophages and bioluminescent method. METHODS: Firstly detected the luciferase activity in different bacteria by using mycobacteriophages, then assessed the best drug concentration of rifampicin for drug susceptibility test in luciferase reporter assay, and lastly used the above conditions to decide rifampicin susceptibilities to different mycobacteria and compare the result with L-J medium culture...
August 2000: Chinese Journal of Tuberculosis and Respiratory Diseases
H Hashimoto, R H Rownd
When Proteus mirabilis harboring the R factor NR1 is cultured in Penassay broth containing 100 mug of chloramphenicol (CM) per ml, there is an amplification in the number of copies of the r-determinants per cell. Under these conditions, R factors harboring multiple tandem sequences of r-determinants are formed. Autonomous poly-f-determinants consisting of multiple copies of r-determinants are also formed. This phenomenon has been referred to as the "transition". Transitioned cells have considerably higher levels of resistance to CM and streptomycin (SM), but not to tetracycline (TC), than do nontransitioned cells and grow more rapidly in medium containing either CM or SM...
July 1975: Journal of Bacteriology
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