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https://www.readbyqxmd.com/read/28735330/expiration-date-prediction-of-biocontrol-agent-prepared-with-bacillus-subtilis-b579-using-the-accelerated-aging-method
#1
Yu Zheng, Xiaoyang Jiao, Fang Chen, Xuelian Wang, Min Wang
The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 10(11) CFU/g. It is calculated that the expiration date of the agent was about 17 months at 25°C. During this period, the available bacteria retained more than 90% of the value in the initial product. Thus, this work suggests the expiration date of biocontrol agents composed with spores could be estimated using the accelerated aging method...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28733288/bacillomycin-d-produced-by-bacillus-amyloliquefaciens-is-involved-in-the-antagonistic-interaction-with-the-plant-pathogenic-fungus-fusarium-graminearum
#2
Qin Gu, Yang Yang, Qiming Yuan, Guangming Shi, Liming Wu, Zhiying Lou, Rong Huo, Huijun Wu, Rainer Borriss, Xuewen Gao
Fusarium graminearum (teleomorph: Ascomycota, Hypocreales, Gibberella, Gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. Controlling this toxin-producing pathogen is a significant challenge. In the present study, the commercially available strain Bacillus amyloliquefaciens (Bacteria, Firmicutes, Bacillales, Bacillus) FZB42 showed strong activity against F. graminearum The lipopeptide, bacillomycin D, produced by FZB42, was proven to contribute to the antifungal activity...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28730550/disease-management-of-tomato-through-pgpb-current-trends-and-future-perspective
#3
REVIEW
Vipin Kumar Singh, Amit Kishore Singh, Ajay Kumar
Tomato is the world's second most cultivated vegetable. During cultivation or post-harvest storage, it is susceptible to more than 200 diseases caused by an array of pathogenic fungi, nematodes, bacteria, and viruses. Although wide range of chemical pesticides are currently available to manage plant diseases, continuous application of pesticides not only affect the nutritional contents of tomato but also the texture or productivity of soil. In this context, plant growth promoting bacteria (PGPB) are one of the nature friendly, safe, and effective alternatives for the management of diseases and pathogens of tomato...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28729855/desirable-traits-of-a-good-biocontrol-agent-against-verticillium-wilt
#4
REVIEW
Silke Deketelaere, Lien Tyvaert, Soraya C França, Monica Höfte
The soil-borne fungus Verticillium causes serious vascular disease in a wide variety of annual crops and woody perennials. Verticillium wilt is notoriously difficult to control by conventional methods, so there is great potential for biocontrol to manage this disease. In this study we aimed to review the research about Verticillium biocontrol to get a better understanding of characteristics that are desirable in a biocontrol agent (BCA) against Verticillium wilt. We only considered studies in which the BCAs were tested on plants...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28726267/day-length-constrains-the-time-budget-of-aphid-predators
#5
Jens Joschinski, Tim Kiess, Jochen Krauss
Phenology shifts and range expansions cause organisms to experience novel day length - temperature correlations. Depending on the temporal niche, organisms may benefit or suffer from changes in day length, thus potentially affecting phenological adaptation. We assessed the impact of day length changes on larvae of Chrysoperla carnea (Stephens) and Episyrphus balteatus (De Geer), both of which prey on aphids. Larvae of E. balteatus are night-active, whereas those of C. carnea appear to be crepuscular. We subjected both species in climate chambers to day lengths of 16 : 8 LD and, to circumvent diapause responses, 20 : 4 LD...
July 20, 2017: Insect Science
https://www.readbyqxmd.com/read/28720287/occurrence-toxicity-bioaccessibility-and-mitigation-strategies-of-beauvericin-a-minor-fusarium-mycotoxin
#6
REVIEW
C Luz, F Saladino, F B Luciano, J Mañes, G Meca
Emerging Fusarium mycotoxins include the toxic secondary metabolites fusaproliferin, enniatins, beauvericin (BEA), and moniliform. BEA is produced by some entomo- and phytopathogenic Fusarium species and occurs naturally on corn and corn-based foods and feeds infected by Fusarium spp. BEA has shown various biological activities (antibacterial, antifungal, and insecticidal) and possesses toxic activity, including the induction of apoptosis, increase cytoplasmic calcium concentration and lead to DNA fragmentation in mammalian cell lines...
July 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28713362/biocontrol-agents-increase-the-specific-rate-of-patulin-production-by-penicillium-expansum-but-decrease-the-disease-and-total-patulin-contamination-of-apples
#7
Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang, Maurice T Apaliya, Giuseppe Ianiri, Hongyin Zhang, Raffaello Castoria
Synthetic fungicides are commonly employed for the control of postharvest diseases of fruits. However, due to health concerns about the use of these chemicals, alternative control methods including biocontrol based on antagonistic yeasts are gaining in popularity. In this study, we investigated the effects of two biocontrol yeasts, Rhodotorula mucilaginosa strain 3617 and Rhodotorula kratochvilovae strain LS11, on blue mold and patulin (PAT) contamination caused by Penicillium expansum strains PY and FS7 in artificially inoculated Fuji apples stored at 20°C for 9 days...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28703431/a-matter-of-hierarchy-activation-of-orfamide-production-by-the-posttranscriptional-gac-rsm-cascade-of-pseudomonas-protegens-cha0-through-expression-upregulation-of-the-two-dedicated-transcriptional-regulators
#8
Patricio Martín Sobrero, Andrés Muzlera, Julieta Frescura, Edgardo Jofré, Claudio Valverde
In this work, we surveyed the genome of P. protegens CHA0 in order to identify novel mRNAs possibly under the control of the Gac-Rsm cascade that might, for their part, serve to elucidate as-yet-unknown functions involved in the biocontrol of plant pathogens and/or in cellular processes required for fitness in natural environments. In view of the experimental evidence from former studies on the Gac-Rsm cascade, we developed a computational screen supported by a combination of sequence, structural, and evolutionary constraints that led to a dataset of 43 potential novel mRNA targets...
July 13, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28702137/phage-therapy-assessment-of-the-efficacy-of-a-bacteriophage-isolated-in-the-treatment-of-salmonellosis-induced-by-salmonella-enteritidis-in-mice
#9
Farhad Nikkhahi, Mohammad Mehdi Soltan Dallal, Mahmood Alimohammadi, Abbas Rahimi Foroushani, Zahra Rajabi, Fatemeh Fardsanei, Seyed Mostafa Imeni, Parisa Torabi Bonab
AIM: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected. BACKGROUND: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections...
2017: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28701877/endophytic-fungi-harbored-in-panax-notoginseng-diversity-and-potential-as-biological-control-agents-against-host-plant-pathogens-of%C3%A2-root-rot-disease
#10
You-Kun Zheng, Cui-Ping Miao, Hua-Hong Chen, Fang-Fang Huang, Yu-Mei Xia, You-Wei Chen, Li-Xing Zhao
BACKGROUND: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy Panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of P. notoginseng. METHODS: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of P...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28699822/a-bioencapsulation-and-drying-method-increases-shelf-life-and-efficacy-of-metarhizium-brunneum-conidia
#11
Michael Przyklenk, Marina Vemmer, Miriam Hanitzsch, Anant Patel
This study reports the development of encapsulated and dried entomopathogenic fungus Metarhiuzm brunneum with reduced conidia content, increased conidiation, a high drying survival and enhanced shelf life. Dried beads prepared with the fillers corn starch, potato starch, carboxymethylcellulose or autoclaved baker's yeast, showed enhanced survival with increasing filler content. The maximum survival of 82% was found for beads with 20% corn starch at <0.1 water activity. While increasing starch content inhibits the conidiation, autoclaved baker´s yeast and a combination with starch enhanced the conidiation to 1...
July 12, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28696370/strategies-for-pathogen-biocontrol-using-lactic-acid-bacteria-and-their-metabolites-a-focus-on-meat-ecosystems-and-industrial-environments
#12
REVIEW
Patricia Castellano, Mariana Pérez Ibarreche, Mariana Blanco Massani, Cecilia Fontana, Graciela M Vignolo
The globalization of trade and lifestyle ensure that the factors responsible for the emergence of diseases are more present than ever. Despite biotechnology advancements, meat-based foods are still under scrutiny because of the presence of pathogens, which causes a loss of consumer confidence and consequently a fall in demand. In this context, Lactic Acid Bacteria (LAB) as GRAS organisms offer an alternative for developing pathogen-free foods, particularly avoiding Listeria monocytogenes, with minimal processing and fewer additives while maintaining the foods' sensorial characteristics...
July 11, 2017: Microorganisms
https://www.readbyqxmd.com/read/28695465/trichoderma-for-climate-resilient-agriculture
#13
REVIEW
Prem Lal Kashyap, Pallavi Rai, Alok Kumar Srivastava, Sudheer Kumar
Climate change is one of the biggest challenges of the twenty-first century for sustainable agricultural production. Several reports highlighted the need for better agricultural practices and use of eco-friendly methods for sustainable crop production under such situations. In this context, Trichoderma species could be a model fungus to sustain crop productivity. Currently, these are widely used as inoculants for biocontrol, biofertilization, and phytostimulation. They are reported to improve photosynthetic efficiency, enhance nutrient uptake and increase nitrogen use efficiency in crops...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28695387/the-role-of-leaf-volatiles-of-ludwigia-octovalvis-jacq-raven-in-the-attraction-of-altica-cyanea-weber-coleoptera-chrysomelidae
#14
Saubhik Mitra, Amarnath Karmakar, Abhishek Mukherjee, Anandamay Barik
Larvae and adults of Altica cyanea (Weber) (Coleoptera: Chrysomelidae) feed on the rice-field weed Ludwigia octovalvis (Jacq.) Raven (Onagraceae), commonly known as willow primrose, which is considered a biocontrol agent of the weed. Volatile organic compounds from undamaged plants, plants after 4, 12, and 36 h of continuous feeding by A. cyanea larvae or adult females and after mechanical damaging were identified by GC-MS and GC-FID analyses. Twenty nine compounds were identified from undamaged plants. 2Z-Penten-1-ol, geraniol, and 1-tridecanol were present in all plants damaged by larvae...
July 10, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28691813/microbiome-mediated-biotransformation-of-cane-toad-bufagenins
#15
Venkatanambi Kamalakkannan, Angela A Salim, Robert J Capon
Cane toads are an invasive pest species in which all life stages employ cardiotoxic bufagenins as a chemical defense against predators. Curiously, the bufagenin profiles of eggs and tadpoles are more complex than those of parotoid secretion, the principle mechanism of toxin delivery in adult toads. In an effort to understand this complexity, we determined that selected strains of adult toad parotoid-gland-associated Gram-positive bacteria, Bacillus spp., were capable of biotransforming secreted bufagenins, marinobufagenin (1), telocinobufagenin (2), bufalin (3), and resibufagenin (4), to hydroxylated scaffolds commonly encountered in cane toad eggs and tadpoles...
July 10, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28687515/effect-of-a-pseudomonas-fluorescens-tailocin-against-phytopathogenic-xanthomonas-observed-by-atomic-force-microscopy
#16
Maricruz Fernandez, Agustina Godino, Analía Príncipe, Gustavo M Morales, Sonia Fischer
Phage tail-like bacteriocins, called tailocins, represent a class of protein complexes produced by a multitude of bacteria. Pseudomonas fluorescens SF4c, a strain isolated from wheat rhizosphere, produces a bacteriocin similar to phage tail-like pyocins of Pseudomonas aeruginosa. This tailocin has antimicrobial activity against several phytopathogenic strains of the genus Xanthomonas and Pseudomonas. In this work, the effect of the SF4c tailocin on the phytopathogenic strain X. axonopodis pv vesicatoria Xcv Bv5-4a was analyzed through Atomic Force Microscopy (AFM)...
July 5, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28686086/a-risk-assessment-model-for-bacterial-leaf-spot-bls-of-pepper-capsicum-annuum-l-caused-by-xanthomonas-euvesicatoria-based-on-concentrations-of-macronutrients-and-micronutrients-and-micronutrient-ratios
#17
Bhabesh Dutta, David B Langston, Xuelin Luo, Jeremy Kichler, Scott Carlson, Ron Gitaitis
The phytopathogenic bacterium Xanthomonas euvesicatoria causes bacterial leaf spot (BLS) of pepper and has a worldwide distribution. BLS is difficult to control and an integrated management strategy that incorporates crop rotation, use of clean seeds and clean plants, weed control, resistant varieties, and applications of bactericides, biocontrol agents, and systemic acquired resistance (SAR) inducers is generally recommended. However, even with that arsenal of weapons, BLS can still be responsible for severe losses under favorable environmental conditions...
July 7, 2017: Phytopathology
https://www.readbyqxmd.com/read/28677248/identification-of-suitable-reference-genes-during-the-formation-of-chlamydospores-in-clonostachys-rosea-67-1
#18
Jun Zhang, Zhanbin Sun, Shidong Li, Manhong Sun
Clonostachys rosea is a potential biocontrol fungus that can produce highly resistant chlamydospores under specific conditions. To investigate the genes related to chlamydospore formation, we identified reliable reference genes for quantification of gene expression in C. rosea 67-1 during sporulation. In this study, nine reference genes, actin (ACT), elongation factor 1 (EF1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone (HIS), RNA polymerase II CTD phosphatase Fcp1 (RPP), succinate-semialdehyde dehydrogenase (SSD), TATA-binding protein (TBP), ubiquitin (UBQ), and ubiquitin-conjugating enzyme (UCE), were selected and cloned from 67-1, and their expression stability during chlamydospore formation was determined using reverse transcription quantitative PCR and assessed using the software geNorm, NormFinder and BestKeeper...
July 5, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28676112/unusual-acylation-of-chloramphenicol-in-lysobacter-enzymogenes-a-biocontrol-agent-with-intrinsic-resistance-to-multiple-antibiotics
#19
Wei Zhang, Justin Huffman, Shengying Li, Yuemao Shen, Liangcheng Du
BACKGROUND: The environmental gliding bacteria Lysobacter are emerging as a new group of biocontrol agents due to their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsically resistant to many antibiotics, but the mechanisms behind the antibiotic resistance have not been investigated. RESULTS: Previously, we have used chloramphenicol acetyltransferase gene (cat) as a selection marker in genetic manipulation of natural product biosynthetic genes in Lysobacter, because chloramphenicol is one of the two common antibiotics that Lysobacter are susceptible to...
July 4, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28672808/ecosystem-based-incorporation-of-nectar-producing-plants-for-stink-bug-parasitoids
#20
REVIEW
Glynn Tillman
Adult parasitoids of pest insects rely on floral resources for survival and reproduction, but can be food-deprived in intensively managed agricultural systems lacking these resources. Stink bugs are serious pests for crops in southwest Georgia. Provisioning nectar-producing plants for parasitoids of stink bugs potentially can enhance biocontrol of these pests. Knowledge of spatial and temporal availability and distribution of stink bugs in host plants is necessary for appropriate timing and placement of flowering plants in agroecosystems...
June 24, 2017: Insects
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