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https://www.readbyqxmd.com/read/28944564/harzianone-biosynthesis-by-the-biocontrol-fungus-trichoderma
#1
Lena Barra, Jeroen Sidney Dickschat
Analysis of the volatile terpenes produced by seven fungal strains of the genus Trichoderma by use of a closed-loop stripping apparatus (CLSA) revealed a common production of harzianone, a bioactive, structurally unique diterpenoid consisting of a fused tetracyclic 4,5,6,7-membered ring system. The terpene cyclization mechanism was studied by feeding experiments using selectively 13C- and 2H-labeled synthetic mevalonolactone isotopologues, followed by analysis of the incorporation patterns by 13C-NMR spectroscopy and GC/MS...
September 25, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28943971/genome-comparison-of-pseudomonas-fluorescens-um270-with-related-fluorescent-strains-unveils-genes-involved-in-rhizosphere-competence-and-colonization
#2
Julie E Hernández-Salmerón, Gabriel Moreno-Hagelsieb, Gustavo Santoyo
Pseudomonas fluorescens UM270 is a rhizosphere-colonizing bacterium that produces multiple diffusible and volatile compounds involved in plant growth-promoting activities. Strain UM270 exhibits excellent biocontrol capacities against diverse fungal pathogens . In a previous study, the general UM270 genome characteristics were published. Here, we report a deeper analysis of its gene content and compare it to other P. fluorescens strains to unveil the genetic elements that might explain UM270's great colonizing and plant growth-promoting capabilities...
2017: Journal of Genomics
https://www.readbyqxmd.com/read/28942843/differential-antagonistic-responses-of-bacillus-pumilus-msua3-against-rhizoctonia-solani-and-fusarium-oxysporum-causing-fungal-diseases-in-fagopyrum-esculentum-moench
#3
Mohit Agarwal, Shrivardhan Dheeman, Ramesh Chand Dubey, Pradeep Kumar, Dinesh Kumar Maheshwari, Vivek K Bajpai
Chitinase and surfactin-mediated biocontrol of Rhizoctonia solani and Fusarium oxysporum causing wilt and root rot of Fagopyrum esculentum respectively has been studied in this communication. Bacillus pumilus MSUA3 as a potential bacterial strain strongly inhibited the growth of R. solani and F. oxysporum involving the chitinolytic enzymes and an antibiotic surfactin. Plant growth promoting attributes seem to be involved in plant growth promotion and yield attributes. The action of cell-free culture supernatant (CFCS) was found deleterious to F...
December 2017: Microbiological Research
https://www.readbyqxmd.com/read/28942462/fluidised-bed-spray-drying-formulations-of-candida-sake-cpa-1-by-adding-biodegradable-coatings-to-enhance-their-survival-under-stress-conditions
#4
Anna Carbó, Rosario Torres, Josep Usall, Cristina Solsona, Neus Teixidó
The biocontrol agent Candida sake CPA-1 has demonstrated to be effective against several diseases on fruit. However, for application of CPA-1 under field conditions, it was necessary to mix it with a food coating to improve survival under stress conditions, as well as adherence and distribution on fruit surfaces. The objective of this study was to obtain a more competitive formulation under field conditions to be applied independently of any product. To achieve this purpose, the drying process of CPA-1 by a fluidised-bed spray-drying system together with biodegradable coatings was optimised...
September 23, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28941889/biofilm-production-by-aureobasidium-pullulans-improves-biocontrol-against-sour-rot-in-citrus
#5
Mariana Nadjara Klein, Katia Cristina Kupper
The biocontrol efficiency of Aureobasidium pullulans strain ACBL-77 against Geotrichum citri-aurantii, causal agent of sour rot in citrus, and their interactions were evaluated. For this, were evaluated the incorporation of nutrients in optimizing the antagonistic activity of the yeast, the competition for nutrients between microorganisms, the effect of nutrients on yeast cell and biofilm production and their correlation in the biocontrol of disease, the survival of yeast in citrus fruits and the interaction between microorganisms using scanning electron microscopy...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28940009/print-to-detect-a-rapid-and-ultrasensitive-phage-based-dipstick-assay-for-foodborne-pathogens
#6
Hany Anany, Lubov Brovko, Noha K El Dougdoug, Jennifer Sohar, Heather Fenn, Nada Alasiri, Tarik Jabrane, Patrice Mangin, M Monsur Ali, Balamurali Kannan, Carlos D M Filipe, Mansel W Griffiths
Foodborne pathogens are a burden to the economy and a constant threat to public health. The ability to rapidly detect the presence of foodborne pathogens is a vital component of any strategy towards establishing a safe and secure food supply chain. Bacteriophages (phages) are viruses capable of infecting and replicating within bacteria in a strain-specific manner. The ubiquitous and selective nature of phages makes them ideal for the detection and biocontrol of bacteria. Therefore, the objective of this research was to develop and test a phage-based paper dipstick biosensor for the detection of various foodborne pathogens in food matrices...
September 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28939979/relationship-between-oviposition-virulence-gene-expression-and-parasitism-success-in-cotesia-typhae-nov-sp-parasitoid-strains
#7
R Benoist, C Chantre, C Capdevielle-Dulac, M Bodet, F Mougel, P A Calatayud, S Dupas, E Huguet, R Jeannette, J Obonyo, C Odorico, J F Silvain, B Le Ru, L Kaiser
Studying mechanisms that drive host adaptation in parasitoids is crucial for the efficient use of parasitoids in biocontrol programs. Cotesia typhae nov. sp. (Fernández-Triana) (Hymenoptera: Braconidae) is a newly described parasitoid of the Mediterranean corn borer Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae). Braconidae are known for their domesticated bracovirus, which is injected with eggs in the host larva to overcome its resistance. In this context, we compared reproductive success traits of four Kenyan strains of C...
September 22, 2017: Genetica
https://www.readbyqxmd.com/read/28939938/biological-control-of-wilt-disease-complex-on-tomato-crop-caused-by-meloidogyne-javanica-and-fusarium-oxysporum-f-sp-lycopersici-by-verticillium-leptobactrum
#8
Lobna Hajji-Hedfi, Hajer Regaieg, Asma Larayedh, Noura Chihani, Najet Horrigue-Raouani
The efficacy of Verticillium leptobactrum isolate (HR1) was evaluated in the control of root-knot nematode and Fusarium wilt fungus under laboratory and greenhouse conditions. Five concentrations of V. leptobactrum (HR1) isolate were tested for their nematicidal and fungicidal activities against Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici in vitro. Laboratory trials showed that mycelium growth inhibition of Fusarium wilt fungus was correlated to the increase of the concentration of culture filtrate...
September 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28936838/-isolation-and-identification-of-rhizobacteria-rs-3-from-panax-ginseng-and-evaluation-of-its-antagonistic-activity
#9
Tie-Lin Wang, Dong-Yue Zhao, Mei-Lan Chen, Zhong-Hua Ma, Lan-Ping Guo
A rhizobacteria strain named RS-3 exhibited inhibitory activity against all five Panax ginseng pathogens was isolated from the root of P. ginseng. This strain was identified as Bacillus amyloliquefaciens based on its morphological character and 16S rDNA sequence. Antagonistic activity experiments indicated that the strain could strongly suppress Botrytis cinerea Pers with an inhibitory rate of 54.4%, suggesting the potentialities of biocontrol agent against diseases that frequently happen on ginseng.
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28936573/evaluation-of-beauveria-bassiana-infection-in-the%C3%A2-hemolymph-serum-proteins-of-the-housefly-musca-domestica-l-diptera-muscidae
#10
Sapna Mishra, Peeyush Kumar, Anushree Malik
Beauveria bassiana plays a prominent role in biocontrol of houseflies, Musca domestica (L.). Thus, a deeper insight into immune response of M. domestica during B. bassiana infection was warranted to assist the production of more efficient mycoinsecticides. The present study investigates changes in protein profile of M. domestica hemolymph serum post B. bassiana infection using two-dimensional difference gel electrophoresis (2D-DIGE) followed by identification of selected proteins by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)...
September 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28936201/trichoderma-asperelloides-spores-downregulate-dectin1-2-and-tlr2-receptors-of-mice-macrophages-and-decrease-candida-parapsilosis-phagocytosis-independent-of-the-m1-m2-polarization
#11
Andréa G Dos Santos, Érica A Mendes, Rafael P de Oliveira, Ana M C Faria, Aurizangela O de Sousa, Carlos P Pirovani, Fernanda F de Araújo, Andréa T de Carvalho, Marliete Carvalho Costa, Daniel Assis Santos, Quimi V Montoya, Andre Rodrigues, Jane L Dos Santos
The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28931153/the-coffee-berry-borer-coleoptera-curculionidae-in-puerto-rico-distribution-infestation-and-population-per-fruit
#12
Yobana A Mariño, Victor J Vega, José M García, José C Verle Rodrigues, Noelia M García, Paul Bayman
The coffee berry borer (CBB) (Hypothenemus hampei: Ferrar) was first detected in Puerto Rico in 2007. Its distribution since then has been extensive, but not extensively documented. An island-wide survey was carried out from August to November 2014 (the coffee production season) to assess CBB distribution, infestation, and population per fruit. The CBB was well-established throughout the coffee-growing area of Puerto Rico, but was not evenly distributed. Infestation (or percentages of fruits perforated) in sites sampled ranged from 0 to 95%, and CBB number per infested fruit varied from 1 to 34 individuals...
January 1, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28920176/isolation-and-identification-of-antifungal-peptides-from-bacillus-amyloliquefaciens-w10
#13
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography...
September 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28917670/morphological-characterization-and-biocontrol-effects-of-vibrio-vulnificus-phages-against-vibriosis-in-the-shrimp-aquaculture-environment
#14
Pappu Srinivasan, Palaniappan Ramasamy
The re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. In this study, the lytic Vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. Four new V. vulnificus phages were detected from shrimp aquaculture system, named VV1, VV2, VV3 and VV4. All lytic V. vulnificus phages are the Tectiviruses of the family Tectiviridae with typical double layered elongated icosahedral head and tailless morphology...
September 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28912766/environmental-bacteriophages-of-the-emerging-enterobacterial-phytopathogen-dickeya-solani-show-genomic-conservation-and-capacity-for-horizontal-gene-transfer-between-their-bacterial-hosts
#15
Andrew Day, Jiyoon Ahn, Xinzhe Fang, George P C Salmond
Dickeya solani is an economically important phytopathogen widespread in mainland Europe that can reduce potato crop yields by 25%. There are no effective environmentally-acceptable chemical systems available for diseases caused by Dickeya. Bacteriophages have been suggested for use in biocontrol of this pathogen in the field, and limited field trials have been conducted. To date only a small number of bacteriophages capable of infecting D. solani have been isolated and characterized, and so there is a need to expand the repertoire of phages that may have potential utility in phage therapy strategies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28912334/draft-genome-sequence-of-paenibacillus-sp-xy044-a-potential-plant-growth-promoter-isolated-from-a-tea-plant
#16
Huihui Liu, Wenna Shan, Ying Zhou, Xiaomin Yu
Paenibacillus sp. XY044 is an endophytic bacterium isolated from the stem of a tea plant (Camellia sinensis cv. Maoxie). Here, we present the draft genome sequence of XY044, which includes genes encoding features related to plant growth promotion and biocontrol.
September 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28912112/molecular-basis-of-peripheral-olfactory-sensing-during-oviposition-in-the-behavior-of-the-parasitic-wasp-anastatus-japonicus
#17
Yinliang Wang, Qi Chen, Junqi Guo, Jing Li, Jiatong Wang, Ming Wen, Hanbo Zhao, Bingzhong Ren
Anastatus japonicus is a parasitic wasp and natural enemy of the litchi pest Tessaratoma papillosa, and for decades in China, A. japonicus has been mass-reared inside the eggs of Antheraea pernyi to control T. papillosa. A series of experiments was performed to explore the olfactory mechanism underlying the oviposition behavior of A. japonicus. First, a transcriptomic analysis was performed on the antennae of A. japonicus, and the resulting assemblies led to the generation of 70,473 unigenes. Subsequently, 21,368 unigenes were matched to known proteins, 48 odorant receptors (ORs) (including Orco) and 13 antennal ionotropic receptors (IRs) (including the co-receptors IR8a and IR25a) were identified and predicted to form complete open reading frames (ORFs)...
September 11, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28905488/genetically-engineering-better-fungal-biopesticides
#18
REVIEW
Brian Lovett, Raymond John St Leger
Microbial insect pathogens offer an alternative means of pest control with potential to wean our heavy reliance on chemical pesticides. Insect pathogenic fungi play an important natural role in controlling disease vectors and agricultural pests. Most commercial products employ Ascomycetes in the genera Metarhizium and Beauveria. However, their utilization has been limited by inconsistent field results due to sensitivity to abiotic stresses and naturally low virulence. Other naturally occurring biocontrol agents also face these hurdles to successful application, but the availability of complete genomes and recombinant DNA technologies have facilitated design of multiple fungal pathogens with enhanced virulence and stress resistance...
September 14, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28904409/biomedical-applications-of-polyhydroxyalkanoates
#19
REVIEW
Subhasree Ray, Vipin Chandra Kalia
Polyhydroxyalkanoates (PHA) are produced by a large number of microbes under stress conditions such as high carbon (C) availability and limitations of nutrients such as nitrogen, potassium, phosphorus, magnesium, and oxygen. Here, microbes store C as granules of PHAs-energy reservoir. PHAs have properties, which are quite similar to those of synthetic plastics. The unique properties, which make them desirable materials for biomedical applications is their biodegradability, biocompatibility, and non-toxicity...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28900417/integrated-translatome-and-proteome-approach-for-accurate-portraying-of-widespread-multifunctional-aspects-of-trichoderma
#20
REVIEW
Vivek Sharma, Richa Salwan, P N Sharma, Arvind Gulati
Genome-wide studies of transcripts expression help in systematic monitoring of genes and allow targeting of candidate genes for future research. In contrast to relatively stable genomic data, the expression of genes is dynamic and regulated both at time and space level at different level in. The variation in the rate of translation is specific for each protein. Both the inherent nature of an mRNA molecule to be translated and the external environmental stimuli can affect the efficiency of the translation process...
2017: Frontiers in Microbiology
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