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https://www.readbyqxmd.com/read/29786121/genome-sequence-of-the-novel-virulent-bacteriophage-pmbt14-with-lytic-activity-against-pseudomonas-fluorescens-dsm-50090-r
#1
Sabrina Koberg, Stefanie Gieschler, Erik Brinks, Mareike Wenning, Horst Neve, Charles M A P Franz
Psychrotrophic gram-negative Pseudomonas spp. represent a serious problem in the dairy industry as they can cause spoilage of milk and dairy products. Bacteriophages have moved into focus as promising biocontrol agents for such food spoilage bacteria. The virulent Siphoviridae phage PMBT14 was isolated on a mutant variant of P. fluorescens DSM 50090 challenged with an unrelated virulent P. fluorescens DSM 50090 Podoviridae phage (i.e., mutant strain DSM 50090R ). PMBT14 has a 47,820-bp dsDNA genome with 76 predicted open reading frames (ORFs)...
May 21, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29782978/a-potent-chitinase-from-bacillus-subtilis-for-the-efficient-bioconversion-of-chitin-containing-wastes
#2
Di Wang, Anjie Li, Hongyu Han, Tian Liu, Qing Yang
Bioconversion of chitin-containing waste to N-acetyl-d-glucosamine (GlcNAc) is of economic interest. Highly efficient chitinases are thus desirable. Bacillus subtilis is a well-known microorganism for industrial protein production and biocontrol, however its chitinolytic enzyme has not been studied in detail. In this study, BsChi from B. subtilis was recombinantly expressed in Escherichia coli and characterized as a potent chitinase for degrading crystalline chitin substrates such as α-chitin, β-chitin and crab shells...
May 18, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29782023/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference-in-phloem-and-plant-sap-feeding-hemipteran-insects
#3
Saikat Kumar B Ghosh, Wayne B Hunter, Alexis L Park, Dawn E Gundersen-Rindal
Phloem and plant sap feeding insects invade the integrity of crops and fruits to retrieve nutrients, in the process damaging food crops. Hemipteran insects account for a number of economically substantial pests of plants that cause damage to crops by feeding on phloem sap. The brown marmorated stink bug (BMSB), Halyomorpha halys (Heteroptera: Pentatomidae) and the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae) are hemipteran insect pests introduced in North America, where they are an invasive agricultural pest of high-value specialty, row, and staple crops and citrus fruits, as well as a nuisance pest when they aggregate indoors...
May 4, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29780366/biological-control-of-mango-dieback-disease-caused-by-lasiodiplodia-theobromae-using-streptomycete-and-non-streptomycete-actinobacteria-in-the-united-arab-emirates
#4
Fatima H Kamil, Esam E Saeed, Khaled A El-Tarabily, Synan F AbuQamar
Dieback caused by the fungus Lasiodiplodia theobromae is an important disease on mango plantations in the United Arab Emirates (UAE). In this study, 53 actinobacterial isolates were obtained from mango rhizosphere soil in the UAE, of which 35 (66%) were classified as streptomycetes (SA) and 18 (34%) as non-streptomycetes (NSA). Among these isolates, 19 (12 SA and 7 NSA) showed antagonistic activities against L. theobromae associated with either the production of diffusible antifungal metabolites, extracellular cell-wall-degrading enzymes (CWDEs), or both...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29777829/biocontrol-of-botrytis-cinerea-and-calonectria-gracilis-by-eucalypts-growth-promoters-bacillus-spp
#5
Isabel Cristina Padula Paz, Rita de Cássia Madail Santin, Alexandre Martins Guimarães, Osmar Paulo Pereira da Rosa, Maria Carolina Quecine, Michele de Cássia Pereira E Silva, João Lúcio Azevedo, Aida Terezinha Santos Matsumura
The clonal Eucalyptus plants are commonly obtained by vegetative propagation under a protected environment. This system improves the Botrytis cinerea and Calonectria spp infection on the young eucalypts plantings, resulting gray mold and cutting rot respectively. Currently, the unique available control method is based on chemicals. As alternative, novel methods to manage plant diseases, endophytic microorganisms could be an interesting alternative. Thus, we aimed to evaluate endophytic Bacillus isolated from eucalypts as a biocontrol agent against Botrytis cinerea and Calonectria gracilis, important fungal pathogens in the greenhouse, using clonal plantlets of E...
May 16, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29770582/predator-performance-inferring-predator-switching-behaviors-based-on-nutritional-indices-in-a-coccinellid-psylla-aphid-system
#6
M Amin Jalali, M Reza Mehrnejad, Peter C Ellsworth, Fateme Ranjbar, Mahdi Ziaaddini
BACKGROUND: Knowledge on the nutritional ecology of predatory coccinellids is important to selection of efficient biocontrol agents. We examined five species common to pistachio orchards and nutritional indices when fed on the key psylla pest of the system, Agonoscena pistaciae, in contrast to an alternative prey common on herbaceous plants in orchards, Aphis gossypii. RESULTS: Feeding studies revealed that Oenopia conglobata contaminata and Adalia bipunctata may be more efficient as biocontrol agents for A...
May 16, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29770458/surfactant-effects-on-wettability-of-penicillium-frequentans-formulations-to-improve-brown-rot-biocontrol
#7
Belén Guijarro, Inmaculada Larena, Paloma Melgarejo, Antonieta De Cal
BACKGROUND: Penicillium frequentans could be approach to integrated pest management of brown rot, caused by Monilinia spp. Competition is primary mode of biocontrol activity of P. frequentans, and therefore, it must cover the entire fruit where the pathogen is acting. Our objective was to optimize P. frequentans efficacy, by maximizing fruit surface coverage and retention with the antagonist formulation by surfactant incorporation. RESULTS: Sixteen surfactants were assessed at 3-5 different concentrations and performance of disease-management of brown rot...
May 16, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29768137/organic-amendments-beneficial-microbes-and-soil-microbiota-toward-a-unified-framework-for-disease-suppression
#8
Giuliano Bonanomi, Matteo Lorito, Francesco Vinale, Sheridan L Woo
Organic amendments (OAs) and soilborne biocontrol agents or beneficial microbes (BMs) have been extensively studied and applied worldwide in most agriculturally important plant species. However, poor integration of research and technical approaches has limited the development of effective disease management practices based on the combination of these two bio-based strategies. Insights into the importance of the plant-associated microbiome for crop productivity, which can be modified or modulated by introducing OAs and/or BMs, are providing novel opportunities to achieve the goal of long-term disease control...
May 16, 2018: Annual Review of Phytopathology
https://www.readbyqxmd.com/read/29763937/bacteriophage-cocktail-for-biocontrol-of-escherichia-coli-o157-h7-stability-and-potential-allergenicity-study
#9
Karina Ramirez, Carmina Cazarez-Montoya, Hector Samuel Lopez-Moreno, Nohelia Castro-Del Campo
Escherichia coli O157:H7 has become a global public health and a food safety problem. Despite the implementation of control strategies that guarantee the safety in various products, outbreaks persist and new alternatives are necessary to reduce this pathogen along the food chain. Recently, our group isolated and characterised lytic bacteriophages against E. coli O157:H7 with potential to be used as biocontrol agents in food. To this end, phages need certain requirements to allow their manufacture and application...
2018: PloS One
https://www.readbyqxmd.com/read/29757952/novel-n4-like-bacteriophages-of-pectobacterium-atrosepticum
#10
Colin Buttimer, Hanne Hendrix, Alan Lucid, Horst Neve, Jean-Paul Noben, Charles Franz, Jim O'Mahony, Rob Lavigne, Aidan Coffey
Pectobacterium atrosepticum is an economically important phytopathogen that is responsible for potato blackleg and soft rot, and for which current control strategies are limited. In this study, stem samples of potato crops exhibiting blackleg were taken from three farms in Co. Cork, Ireland, and they were found to be infected with P. atrosepticum . Three closely related bacteriophages (phages) that are specific to this phytopathogen were isolated and characterized, namely vB_PatP_CB1, vB_PatP_CB3, and vB_PatP_CB4 (abbreviated as CB1, CB3, and CB4)...
May 14, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29756384/performance-of-metarhizium-anisopliae-treated-foam-in-combination-with-phytoseiulus-longipes-evans-on-tetranychus-evansi-baker-pritchard-acari-tetranychidae
#11
Ginette Y Azandémè Hounmalon, Nguya K Maniania, Saliou Niassy, Simon Fellous, Serge Kreiter, Emilie Delétré, Komi K Fiaboe, Thibaud Martin
BACKGROUND: Tetranychus evansi (Te) is an exotic pest of solanaceous crops in Africa. The predatory mite Phytoseiulus longipes (Pl) and the fungus Metarhizium anisopliae (Ma), are potential biocontrol agents of Te. The present study investigated efficacy of fungus-treated foam placed above or below the third Te-infested tomato leaf. The persistence of fungus-treated foam and the performance of Pl with or without fungus-treated foam were evaluated. RESULTS: The fungus-treated foam was effective when Te infestation was below the third tomato leaf as no damage was recorded on all upper tomato leaves up to 30 days post-treatment...
May 13, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29756353/effect-of-potassium-sorbate-e-202-and-the-antifungal-pgafp-protein-on-aspergillus-carbonarius-growth-and-ochratoxin-a-production-in-raisin-simulating-media
#12
Sihem Fodil, Josué Delgado, Leonardo Varvaro, Thaer Yaseen, Alicia Rodríguez
BACKGROUND: Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium fungi. The presence of OTA in raisins is mainly related to the black Aspergillus spp. contamination. This toxin poses risks to human and animal health due to their high toxicity and carcinogenicity. Therefore, new strategies to avoid the risk associated to OTA are necessary. RESULTS: In the present study, a comparison between the effects of the antifungal protein PgAFP and potassium sorbate (E-202), on the growth of Aspergillus carbonarius, biosynthetic- and stress-related gene expression and its OTA production at two water activity (aw ) levels 0...
May 13, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29755447/stomatal-closure-and-sa-ja-et-signaling-pathways-are-essential-for-bacillus-amyloliquefaciens-fzb42-to-restrict-leaf-disease-caused-by-phytophthora-nicotianae-in-nicotiana-benthamiana
#13
Liming Wu, Ziyang Huang, Xi Li, Liumin Ma, Qin Gu, Huijun Wu, Jia Liu, Rainer Borriss, Zhen Wu, Xuewen Gao
Bacillus amyloliquefaciens FZB42 is a plant growth-promoting rhizobacterium that induces resistance to a broad spectrum of pathogens. This study analyzed the mechanism by which FZB42 restricts leaf disease caused by Phytophthora nicotianae in Nicotiana benthamiana . The oomycete foliar pathogen P. nicotianae is able to reopen stomata which had been closed by the plant innate immune response to initiate penetration and infection. Here, we showed that root colonization by B. amyloliquefaciens FZB42 restricted pathogen-mediated stomatal reopening in N...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29755261/spider-assemblages-associated-with-different-crop-stages-of-irrigated-rice-agroecosystems-from-eastern-uruguay
#14
Leticia Bao, Juaquín Ginella, Mónica Cadenazzi, Enrique A Castiglioni, Sebastián Martínez, Luis Casales, María P Caraballo, Álvaro Laborda, Miguel Simo
The rice crop and associated ecosystems constitute a rich mosaic of habitats that preserve a rich biological diversity. Spiders are an abundant and successful group of natural predators that are considered efficient in the biocontrol of the major insect pests in agroecosystems. Spider diversity in different stages of the rice crop growth from eastern Uruguay was analysed. Field study was developed on six rice farms with rotation system with pasture, installed during intercropping stage as cover crop. Six rice crops distributed in three locations were sampled with pitfall and entomological vaccum suction machine...
2018: Biodiversity Data Journal
https://www.readbyqxmd.com/read/29753873/biodiversity-and-pathogenicity-of-bacteria-associated-with-the-gut-microbiota-of-beet-armyworm-spodoptera-exigua-h%C3%A3-bner-lepidoptera-noctuidae
#15
Ardahan Eski, İsmail Demir, Mustafa Güllü, Zihni Demirbağ
In order to find an effective and environmentally friendly biocontrol agent against Spodoptera exigua, we isolated and identified a total of 15 different bacterial species belonging to phyla Firmicutes, Proteobacteria and Bacteroidetes. According to the phenotypic, genotypic and phylogenetic properties, bacterial isolates were identified as Bacillus cereus (Se1), Lysinibacillus macroides (Se2), Pseudomonas geniculata (Se3), Paenibacillus tylopili (Se4), Staphylococcus succinus (Se5), Acinetobacter soli (Se6), Chryseobacterium indologenes (Se7), Bacillus toyonensis (Se8), Serratia marcescens (Se9), Paenibacillus amylolyticus (Se10), Paenibacillus xylanexedens (Se11), Enterobacter ludwigii (Se12), Bacillus thuringiensis (Se13), Bacillus thuringiensis (Se14) and Lysinibacillus fusiformis (Se15)...
May 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29752875/characterisation-of-antagonistic-yeasts-for-biocontrol-applications-on-apples-or-in-soil-by-quantitative-analyses-of-synthetic-yeast-communities
#16
Simon Gross, Liesa Kunz, Denise C Müller, Amanda Santos Kron, Florian M Freimoser
Antagonistic yeasts suppress plant pathogenic fungi by various mechanisms, but their biocontrol efficacy also depends on the ability to compete and persist in the environment. The goal of the work presented here was to quantify the composition of synthetic yeast communities in order to determine the competitiveness of different species and identify promising candidates for plant protection. For this purpose, colony counting of distinct species and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS; MALDI biotyping) were used to distinguish different yeast species and to quantify the composition of a synthetic community of six yeasts (Aureobasidium pullulans, Candida subhashii, Cyberlindnera sargentensis, Hanseniaspora sp...
May 12, 2018: Yeast
https://www.readbyqxmd.com/read/29751651/clostridium-perfringens-virulent-bacteriophage-cps2-and-its-thermostable-endolysin-lyscps2
#17
Eunsu Ha, Bokyung Son, Sangryeol Ryu
Clostridium perfringens is one of the most common causes of food-borne illness. The increasing prevalence of multidrug-resistant bacteria requires the development of alternatives to typical antimicrobial treatments. Here, we isolated and characterized a C. perfringens -specific virulent bacteriophage CPS2 from chicken feces. The CPS2 phage contains a 17,961 bp double-stranded DNA genome with 25 putative ORFs, and belongs to the Picovirinae , subfamily of Podoviridae . Bioinformatic analysis of the CPS2 genome revealed a putative endolysin, LysCPS2, which is homologous to the endolysin of Clostridium phage phiZP2 and phiCP7R...
May 11, 2018: Viruses
https://www.readbyqxmd.com/read/29750330/antagonism-against-fish-pathogens-by-cellular-components-preparations-of-bacillus-coagulans-mtcc-9872-and-it-s-in-vitro-probiotic-characterisation
#18
Sebastian J Midhun, Sahadevan Neethu, A Vysakh, E K Radhakrishnan, Mathew Jyothis
Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment will be ineffective. Here, we can use probiotic bacteria as a biocontrol agent in fish disease and it is a novel field. In this study, antimicrobial potential of the bacterium Bacillus coagulans (MTCC-9872) has been evaluated through in vitro antagonistic activity of cellular preparations/components against potent pathogens...
May 10, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29749026/molecular-identification-of-cultivable-bacteria-in-the-gut-of-adult-bactrocera-tau-walker-and-their-trapping-effect
#19
Mijuan Luo, Hehe Zhang, Yinggang Du, Atif Idrees, Longyan He, Jiahua Chen, Qing E Ji
BACKGROUND: It has been shown that some bacteria can attract their hosts. Our research aimed to identify cultivable bacterial isolates in the guts of sexually mature adult female and male Bactrocera tau and to evaluate their utility in trapping of B. tau. RESULTS: Forty-one strains of bacteria were isolated and identified from B. tau with morphological, physiological, biochemical and 16S rDNA analysis. The dominant bacterial genera shared by both females and males were Enterobacter, Providencia and Serratia...
May 11, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29743126/selective-flowers-to-attract-and-enhance-telenomus-laeviceps-hymenoptera-scelionidae-a-released-biocontrol-agent-of-mamestra-brassicae-lepidoptera-noctuidae
#20
G Barloggio, L Tamm, P Nagel, H Luka
The importance of the right food source for the survival and reproduction of certain insect species is well documented. In the case of biocontrol agents, this is even more important in order to reach a high predation or parasitation performance. The egg parasitoid Telenomus laeviceps (Förster, 1861) (Hymenoptera: Scelionidae) is a promising candidate for mass release as a biological control agent of the cabbage moth Mamestra brassicae (Linnaeus, 1758) (Lepidoptera: Noctuidae). However, adult T. laeviceps need a sugar-rich food source to increase their parasitation performance and produce a good amount of female offspring...
May 10, 2018: Bulletin of Entomological Research
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