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https://www.readbyqxmd.com/read/29775724/biological-activities-and-phytocompounds-of-northwest-algeria-ajuga-iva-l-extracts-partial-identification-of-the-antibacterial-fraction
#1
Saida Medjeldi, Lamjed Bouslama, Amina Benabdalla, Rim Essid, Soumaya Haou, Salem Elkahoui
The use of synthetic food additive and the appearance of antibiotic resistance are at the basis of important human health problems. The substitution of synthetic compounds with new natural substances extracted from plants or microorganisms is therefore the ideal solution to this scourge. The objective of this work was to evaluate the phyto-constituents (polyphenols, flavonoids and condensed tannins), and to test the biological activities (antioxidant, antibacterial and antiviral) of the Ajuga iva (L) aerial part extracts...
May 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29775704/alkaline-active-cyanide-dihydratase-of-flavobacterium-indicum-mtcc-6936-growth-optimization-purification-characterization-and-in-silico-analysis
#2
Virender Kumar, Vijay Kumar, Tek Chand Bhalla
The present work explores a rare cyanide dihydratase of Flavobacterium indicum MTCC 6936 for its potential of cyanide degradation. The enzyme is purified to 12 fold with a yield of 76%. SDS and native-PAGE analysis revealed that enzyme was monomer of 40 kDa size. The enzyme works well in mesophilic range at wide array of pH. The thermostability profile of cyanide dihydratase revealed that the enzyme is quite stable at 30 °C and 35 °C with half-life of 6 h 30 min and 5 h respectively. Km and Vmax for cyanide dihydratase of F...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775652/advances-and-prospects-of-bacillus-subtilis-cellular-factories-from-rational-design-to-industrial-applications
#3
REVIEW
Yang Gu, Xianhao Xu, Yaokang Wu, Tengfei Niu, Yanfeng Liu, Jianghua Li, Guocheng Du, Long Liu
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations. Till date, B. subtilis has been identified as attractive hosts for the production of recombinant proteins and chemicals. By applying various systems and synthetic biology tools, the productivity features of B. subtilis can be thoroughly analyzed and further optimized via metabolic engineering...
May 15, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29775454/in-vivo-and-in-vitro-studies-of-cry5b-and-nicotinic-acetylcholine-receptor-agonist-anthelmintics-reveal-a-powerful-and-unique-combination-therapy-against-intestinal-nematode-parasites
#4
Yan Hu, Melanie Miller, Bo Zhang, Thanh-Thanh Nguyen, Martin K Nielsen, Raffi V Aroian
BACKGROUND: The soil-transmitted nematodes (STNs) or helminths (hookworms, whipworms, large roundworms) infect the intestines of ~1.5 billion of the poorest peoples and are leading causes of morbidity worldwide. Only one class of anthelmintic or anti-nematode drugs, the benzimidazoles, is currently used in mass drug administrations, which is a dangerous situation. New anti-nematode drugs are urgently needed. Bacillus thuringiensis crystal protein Cry5B is a powerful, promising new candidate...
May 18, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29774899/smartphone-based-rapid-quantification-of-viable-bacteria-by-single-cell-microdroplet-turbidity-imaging
#5
Xiaonan Cui, Lihui Ren, Yufei Shan, Xixian Wang, Zhenlong Yang, Chunyu Li, Jian Xu, Bo Ma
Standard plate count (SPC) has been recognized as the golden standard for the quantification of viable bacteria. However, SPC usually takes one to several days to grow individual cells into a visible colony, which greatly hampers its application in rapid bacteria enumeration. Here we present a microdroplet turbidity imaging based digital standard plate count (dSPC) method to overcome this hurdle. Instead of cultivating on agar plates, bacteria are encapsulated in monodisperse microdroplets for single-cell cultivation...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29774759/melatonin-stimulated-biosynthesis-of-anti-microbial-znonps-by-enhancing-bio-reductive-prospective-in-callus-cultures-of-catharanthus-roseus-var-alba
#6
Hafiza Rida Riaz, Syed Salman Hashmi, Tariq Khan, Christophe Hano, Nathalie Giglioli-Guivarc'h, Bilal Haider Abbasi
Melatonin as plant growth regulator induces differential effects on metabolites that are responsible for reduction, capping and stabilization of zinc oxide nanoparticles. Phytochemical analysis of callus cultures was performed and results were compared with callus cultures supplemented with other plant growth regulators (α-napthalene acetic acid, 2,4-dichlorophenoxy acetic acid and thidiazuron). Highest total phenolic and flavonoid content [42.23 mg of gallic acid equivalent (GAE) g-1 DW and 36.4 mg of (quercetin equivalent) g-1 DW, respectively] were recorded at melatonin (1...
May 18, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29772478/bacillus-thuringiensis-cry5b-protein-as-a-new-pan-hookworm-cure
#7
Yan Hu, Thanh-Thanh Nguyen, Alice C Y Lee, Joseph F Urban, Melanie M Miller, Bin Zhan, David J Koch, Jason B Noon, Ambily Abraham, Ricardo Toshio Fujiwara, Dwight D Bowman, Gary R Ostroff, Raffi V Aroian
Hookworms are intestinal nematode parasites that infect nearly half a billion people and are globally one of the most important contributors to iron-deficiency anemia. These parasites have significant impacts in developing children, pregnant women and working adults. Of all the soil-transmitted helminths or nematodes (STNs), hookworms are by far the most important, with disease burdens conservatively estimated at four million DALYs (Disability-Adjusted Life Years) and with productivity losses of up to US$139 billion annually...
May 4, 2018: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29772463/bioremediation-of-organophosphorus-pesticide-phorate-in-soil-by-microbial-consortia
#8
Monu Jariyal, Vikas Jindal, Kousik Mandal, Virash Kamal Gupta, Balwinder Singh
Microbial consortia isolated from aged phorate contaminated soil were used to degrade phorate. The consortia of three microorganisms (Brevibacterium frigoritolerans, Bacillus aerophilus and Pseudomonas fulva) could degrade phorate, and the highest phorate removal (between 97.65 and 98.31%) was found in soils inoculated with mixed cultures of all the three bacterial species. However, the mixed activity of any of two of these bacteria was lower than mixed consortia of all the three bacterial species. The highest degradation by individual mixed consortia of (B...
May 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29772382/enhanced-biodegradation-of-hydrocarbons-in-petroleum-tank-bottom-oil-sludge-and-characterization-of-biocatalysts-and-biosurfactants
#9
S Hepziba Suganthi, Shabnam Murshid, Sriswarna Sriram, K Ramani
Petroleum hydrocarbon removal from tank bottom oil sludge is a major issue due to its properties. Conventional physicochemical treatment techniques are less effective. Though the bioremediation is considered for the hydrocarbon removal from tank bottom oil sludge, the efficiency is low and time taking due to the low yield of biocatalysts and biosurfactants. The focal theme of the present investigation is to modify the process by introducing the intermittent inoculation for the enhanced biodegradation of hydrocarbons in the tank bottom oil sludge by maintaining a constant level of biocatalysts such as oxidoreductase, catalase, and lipase as well as biosurfactants...
May 14, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29771915/inactivation-of-bpsl1039-1040-atp-binding-cassette-transporter-reduces-intracellular-survival-in-macrophages-biofilm-formation-and-virulence-in-the-murine-model-of-burkholderia-pseudomallei-infection
#10
Peechanika Pinweha, Pornpan Pumirat, Jon Cuccui, Niramol Jitprasutwit, Veerachat Muangsombut, Varintip Srinon, Usa Boonyuen, Parameth Thiennimitr, Paiboon Vattanaviboon, Felipe Cia, Sam Willcocks, Gregory J Bancroft, Brendan W Wren, Sunee Korbsrisate
Burkholderia pseudomallei, a gram-negative intracellular bacillus, is the causative agent of a tropical infectious disease called melioidosis. Bacterial ATP-binding cassette (ABC) transporters import and export a variety of molecules across bacterial cell membranes. At present, their significance in B. pseudomallei pathogenesis is poorly understood. We report here characterization of the BPSL1039-1040 ABC transporter. B. pseudomallei cultured in M9 medium supplemented with nitrate, demonstrated that BPSL1039-1040 is involved in nitrate transport for B...
2018: PloS One
https://www.readbyqxmd.com/read/29771325/native-bacteria-promote-plant-growth-under-drought-stress-condition-without-impacting-the-rhizomicrobiome
#11
Elisabeth Armada, Márcio F A Leite, Almudena Medina, Rosario Azcón, Eiko E Kuramae
Inoculation of plants with beneficial plant growth-promoting bacteria (PGPB) emerges a valuable strategy for ecosystem recovery. However, drought conditions might compromise plant-microbe interactions especially in semiarid regions. This study highlights the effect of native PGPB after one-year inoculation on autochthonous shrubs growth and rhizosphere microbial community composition and activity under drought stress conditions. We inoculated three plant species of semiarid Mediterranean zones, Thymus vulgaris, Santolina chamaecyparissus and Lavandula dentata with a Bacillus thuringiensis strain IAM 12077 and, evaluated the impact on plant biomass, plant nutrient contents, arbuscular mycorrhiza fungi (AMF) colonization, soil rhizosphere microbial activity, and both the bacterial and fungal communities...
May 16, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29770716/new-pyrazinoquinazoline-alkaloids-isolated-from-a-culture-of-stenotrophomonas-maltophilia-qb-77
#12
Sheng-Yan Qian, Cai-Ling Yang, Afsar Khan, Rong-Xiang Chen, Ming-Song Wu, Li Tuo, Qian Wang, Jian-Guo Liu, Gui-Guang Cheng
Two new pyrazinoquinazoline alkaloids, epi-fiscalin D (1) and epi-fiscalin E (2), as well as three known analogues, norquinadoline A (3), quinadoline A (4), and fiscalin C (5), were isolated from ethyl acetate extract of the fermentation broth of Stentrophomonas maltophilia QB-77. The structures of new compounds were elucidated on the basis of extensive spectroscopic data analysis including UV, HRESIMS, and 1D and 2D NMR experiments. All the isolated compounds were tested for their in vitro cytotoxicity against five human cancer cell lines (SMMC-7721, MCF-7, HL-60, SW480, and A-549) and antibacterial activities against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus...
May 17, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29770713/component-composition-of-the-extracts-and-essential-oils-from-the-alhagi-canescens-growing-in-uzbekistan-and-their-antimicrobial-activity
#13
Sabir Nishanbaev, Khayrulla Bobakulov, Bakhodir Okhundedaev, Sobirdjan Sasmakov, Elvira Yusupova, Shakhnoz Azimova, Nasrulla Abdullaev
The volatile compounds of hexane, benzene extracts and essential oils (EOs) isolated by steam- and hydrodistillation methods from aerial part of Alhagi canescens were studied by GC-MS analysis. Seventeen components were found in the hexane and benzene extracts, among them palmitic acid (25.2 and 22.1%), neophytadiene (7.3 and 22.3%), cis-chrysanthenyl acetate (11.0% in benzene), cis-geranyl acetate (7.8% in benzene) were major components. The first time fifty-six volatile compounds were identified in the EOs and camphor (5...
May 17, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29770266/renal-granulomatosis-post-intravesical-bacillus-calmette-guerin-therapy-for-non-muscle-invasive-bladder-cancer
#14
Karen Tran-Harding, Rashmi T Nair, Halemane Ganesh
Intravesical Bacillus Calmette-Guerin (BCG) immunotherapy is a proven, effective treatment for intermediate- and high-risk non-muscle-invasive bladder cancer. Minor side effects are common and expected but systemic effects can occur in <5% of treated patients. We present a rare case of a 49-year-old male that presented with fever and chills after 3 weeks of intravesical BCG therapy post transurethral resection of bladder tumor. New renal lesions were present on contrast-enhanced computed tomography scan which was histologically proven to be necrotizing renal granulomatosis...
2018: Journal of Clinical Imaging Science
https://www.readbyqxmd.com/read/29769322/insights-into-the-regulatory-function-of-the-%C3%A9-subunit-from-bacterial-f-type-atp-synthases-a-comparison-of-structural-biochemical-and-biophysical-data
#15
REVIEW
Alexander Krah, Mariel Zarco-Zavala, Duncan G G McMillan
ATP synthases catalyse the formation of ATP, the most common chemical energy storage unit found in living cells. These enzymes are driven by an electrochemical ion gradient, which allows the catalytic evolution of ATP by a binding change mechanism. Most ATP synthases are capable of catalysing ATP hydrolysis to varying degrees, and to prevent wasteful ATP hydrolysis, bacteria and mitochondria have regulatory mechanisms such as ADP inhibition. Additionally, ɛ subunit inhibition has also been described in three bacterial systems, Escherichia coli , Bacillus PS3 and Caldalkalibacillus thermarum TA2...
May 2018: Open Biology
https://www.readbyqxmd.com/read/29768888/effect-of-surfactants-on-the-structure-and-adsorption-efficiency-of-hydroxyapatite-nanorods
#16
Manisha Sharma, Amit Mishra, Akansha Mehta, Diptiman Choudhury, Soumen Basu
Porous hydroxyapatite (HAp) nanorods using surfactant templating proceeded via microwave irradiation method. Study of BET surface area measurement of the HAp nanorods showed that surface area of HAp nanorods with a mixture of cat-anionic surfactants was higher (56.16 m2/g) than their individual counterpart (for anionic 52.8 m2/g and for cationic 48.8 m2/g) as well as without surfactant (19.07 m2/g) due to higher synergistic effect and low critical aggregation concentration of the mixture. Surfactant-directed synthesis of porous HAp has been explored in literature, but the relation between the pore size distribution, surface area and morphology and choice of surfactant(s) was not fully understood and hence in this work we have explored those parameters...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29768238/one-pot-green-fabrication-of-metallic-silver-nanoscale-materials-using-crescentia-cujete-l-and-assessment-of-their-bactericidal-activity
#17
Seetharaman Prabukumar, Chandrasekaran Rajkuberan, Gnanasekar Sathishkumar, Mani Illaiyaraja, Sivaperumal Sivaramakrishnan
In this study, the leaf extract of an important medicinal plant Crescentia cujete L. (CC) was employed as a green reducing agent to synthesise highly-stable C. cujete silver nanoparticles (CCAgNPs). The reduction of Ag+ to Ag0 nanoparticles was initially observed by a colour change which generates an intense surface plasmon resonance peak at 417 nm using a UV-Vis spectrophotometer. Various optimisation factors such as temperature, pH, time and the stoichiometric proportion of the reaction mixture were performed, which influence the size, dispersity and synthesis rate of CCAgNPs...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29768235/green-synthesis-of-silver-nanoparticles-with-the-arial-part-of-dorema-ammoniacum-d-extract-by-antimicrobial-analysis
#18
Fakhte Zandpour, Ali Reza Allafchian, Mohammad Reza Vahabi, Seyed Amir Hossein Jalali
Silver nanoparticles (SNPs) were synthesised by using the Arial part extract of Dorema ammoniacum D . and characterised by employing UV-visible spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction techniques. Transmission electron microscopy and field emission scanning electron microscopy were applied to investigate the morphological structure of the bio-synthesised SNPs. The antimicrobial activity of SNPs was studied against Gram positive ( Bacillus cereus and Staphylococcus aureus ) and Gram-negative ( Escherichia coli and Salmonella typhimurium ) bacteria by employing the disk diffusion agar process...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29767964/a-synthetic-polymer-affinity-ligand-for-bacillus-thuringiensis-bt-cry1ab-ac-protein-the-use-of-biomimicry-based-on-the-bt-protein-insect-receptor-binding-mechanism
#19
Mingming Liu, Rong Huang, Adam C Weisman, Xiaoyang Yu, Shih-Hui Lee, Yalu Chen, Chao Huang, Senhua Hu, Xiuhua Chen, Wenfeng Tan, Fan Liu, Hao Chen, Kenneth J Shea
We report a novel strategy for creating abiotic Bacillus thuringiensis (Bt) protein affinity ligands by biomimicry of the recognition process that takes place between Bt Cry1Ab/Ac proteins and insect receptor cadherin-like Bt-R1 proteins. Guided by this strategy, a library of synthetic polymer nanoparticles (NPs) was prepared and screened for binding to three epitopes 280FRGSAQGIEGS290, 368RRPFNIGINNQQ379 and 436FRSGFSNSSVSIIR449 located in loop α8, loop 2 and loop 3 of domain II of Bt Cry1Ab/Ac proteins. A negatively charged and hydrophilic nanoparticle (NP12) was found to have high affinity to one of the epitopes, 368RRPFNIGINNQQ379...
May 16, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29767789/effects-of-tannic-acid-extract-on-performance-and-intestinal-health-of-broiler-chickens-following-coccidiosis-vaccination-and-or-a-mixed-species-eimeria-challenge
#20
R M Tonda, J K Rubach, B S Lumpkins, G F Mathis, M J Poss
Two experiments were conducted to investigate the effects of tannic acid extract (TAE) formulations on the performance and intestinal health of male Cobb × Cobb 500 broilers exposed to coccidiosis. In the first experiment, 320 broiler chicks were randomly assigned to 5 treatments with 8 replicates. Treatments included non-medicated, uninfected (NC); non-medicated, infected (PC); salinomycin (SAL, 66 mg/kg); tannic acid (TA, 0.5 g/kg) and TAE (TAE, 0.5 g/kg). On d 14, all groups (except NC) were orally inoculated with Eimeria acervulina, E...
May 15, 2018: Poultry Science
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