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https://www.readbyqxmd.com/read/28651380/utilization-of-acid-pre-treated-coconut-dregs-as-a-substrate-for-production-of-detergent-compatible-lipase-by-bacillus-stratosphericus
#1
Nur Bainun Mohd Zin, Busyra Mohamad Yusof, Siti Nurbaya Oslan, Helmi Wasoh, Joo Shun Tan, Arbakariya B Ariff, Murni Halim
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28651198/the-toxicity-of-cationic-surfactant-hdtma-br-desorbed-from-surfactant-modified-zeolite-towards-faecal-indicator-and-environmental-microorganisms
#2
Peter J Reeve, Howard J Fallowfield
Surfactant Modified Zeolite (SMZ) represents a versatile, cost-effective permeable reactive material, capable of treating multiple classes of contaminants. The potential for HDTMA-Br, a cationic surfactant commonly used to modify zeolite, to desorb from the zeolite surface has been identified as a potential issue for the ongoing use of SMZ in water remediation contexts. This paper investigates the toxicity of HDTMA-Br towards enteric virus surrogates, F-RNA bacteriophage MS2 and E. coli, Bacillus subtilis, and soil microflora...
June 13, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28651141/a-comparative-study-of-pilot-scale-bio-trickling-filters-with-counter-and-cross-current-flow-patterns-in-the-treatment-of-emissions-from-chemical-fibre-wastewater-treatment-plant
#3
Zhuhui Yang, Jia Liu, Jingyang Cao, Dinghe Sheng, Tingjian Cai, Jian Li
Two pilot-scale bio-trickling filters (BTFs) with counter-current and cross-current flow modes were constructed, and their performance tested, for purifying chemical fibre waste gas containing H2S, NH3 and VOCs with a maximum gas flow rate of 1008m(3)h(-1). The counter-current type of BTF presented with superior biodegradation results compared to the cross-current type: it could start up quickly, tolerated high transient shock loadings, and possessed an average contaminant removal efficiency higher than 90% with an empty bed residence time of 59s...
June 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651081/inactivation-of-pathogenic-microorganisms-in-freshwater-using-hso5-uv-a-led-and-hso5-m-n-uv-a-led-oxidation-processes
#4
Jorge Rodríguez-Chueca, Tatiana Silva, José R Fernandes, Marco S Lucas, Gianluca Li Puma, José A Peres, Ana Sampaio
Freshwater disinfection using photolytic and catalytic activation of peroxymonosulphate (PMS) through PMS/UV-A LED and PMS/M(n+)/UV-A LED [M(n+) = Fe(2+) or Co(2+)] processes was evaluated through the inactivation of three different bacteria: Escherichia coli (Gram-negative), Bacillus mycoides (sporulated Gram-positive), Staphylococcus aureus (non-sporulated Gram-positive), and the fungus Candida albicans. Photolytic and catalytic activation of PMS were effective in the total inactivation of the bacteria using 0...
June 10, 2017: Water Research
https://www.readbyqxmd.com/read/28650935/fulminant-sepsis-due-to-granulibacter-bethesdensis-in-a-4-year-old-boy-with-x-linked-chronic-granulomatous-disease
#5
Erick F Mayer, Philip Gialanella, Iona Munjal, Charlotte Cunningham-Rundles, Jasmeen Dara
Granulibacter bethesdensis is a Gram-negative bacillus described as a pathogen exclusively in patients with chronic granulomatous disease (CGD), a phagocytic disorder that impairs the ability to clear catalase-producing organisms. Granulibacter usually causes chronic and recurrent lymphadenopathies. We report the fatal case of a 4-year-old boy with CGD, who presented with sepsis after a few days of abdominal pain and diarrhea.
June 23, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28650753/lower-extremity-infections-caused-by-serratia-marcescens-a-report-of-three-cases-and-a-literature-review
#6
Luis Marin, Raymond Rowan, Ana Mantilla, Bamidele Olupona, Ann MacIntyre
Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has been cited to cause infection in immunocompromised populations. In the literature, S marcescens infections of the lower extremity have presented as granulomatous ulceration, abscess, bullous cellulitis, and necrotizing fasciitis. Herein we present a series of three cases of lower-extremity infections in which S marcescens was the sole or a contributing pathogen. We discuss the commonalities of these three cases as well as with those previously cited...
May 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/28650741/microbial-culturomics-application-for-global-health-noncontiguous-finished-genome-sequence-and-description-of-pseudomonas-massiliensis-strain-cb-1-t-sp-nov-in-brazil
#7
Lucie Bardet, Teresa Cimmino, Clémence Buffet, Caroline Michelle, Jaishriram Rathored, Fatalmoudou Tandina, Jean-Christophe Lagier, Saber Khelaifia, Jônatas Abrahão, Didier Raoult, Jean-Marc Rolain
Culturomics is a new postgenomics field that explores the microbial diversity of the human gut coupled with taxono-genomic strategy. Culturomics, and the microbiome science more generally, are anticipated to transform global health diagnostics and inform the ways in which gut microbial diversity contributes to human health and disease, and by extension, to personalized medicine. Using culturomics, we report in this study the description of strain CB1(T) ( = CSUR P1334 = DSM 29075), a new species isolated from a stool specimen from a 37-year-old Brazilian woman...
June 26, 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28650559/the-antibacterial-activity-and-modes-of-li-f-type-antimicrobial-peptides-against-bacillus-cereus-in-vitro
#8
Jinzhi Han, Shengming Zhao, Zhi Ma, Ling Gao, Hongxia Liu, Umair Muhammad, Zhaoxin Lu, Fengxia Lv, Xiaomei Bie
AIMS: LI-Fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (Gram-positive bacteria and fungi). In this study, LI-F type antimicrobial peptides (AMP-jsa9) composing of LI-F03a, LI-F03b, LI-F04a, LI-F04b, and LI-F05b were isolated from Paenibacillus polymyxa JSA-9. To better understand the antimicrobial mechanism of AMP-jsa9, the potency and action(s) of AMP-jsa9 against B. cereus were examined. METHODS AND RESULTS: Flow cytometry, confocal laser microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM) observation, as well as determination of peptidoglycan and cell wall associated protein, and other methods were used...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650512/elucidating-sequence-and-solvent-specific-design-targets-to-protect-and-stabilize-enzymes-for-biocatalysis-in-ionic-liquids
#9
K G Sprenger, J G Plaks, J L Kaar, J Pfaendtner
For many different frameworks, the structure, function, and dynamics of an enzyme is largely determined by the nature of its interactions with the surrounding host environment, thus a molecular level understanding of enzyme/host interactions is essential to the design of new processes and applications. Ionic liquid (IL) solvents are a popular class of solvents in which to study enzyme behavior, yet it is still not possible to predict how a given enzyme will behave in a given IL solvent. Furthermore, a dearth of experimental data with which to evaluate simulation force fields has prevented the full integration of experimental and computational techniques to gain a complete picture of enzyme/IL interactions...
June 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28650339/ilc2-modulated-t-cell-to-mdsc-balance-is-associated-with-bladder-cancer-recurrence
#10
Mathieu F Chevalier, Sara Trabanelli, Julien Racle, Bérengère Salomé, Valérie Cesson, Dalila Gharbi, Perrine Bohner, Sonia Domingos-Pereira, Florence Dartiguenave, Anne-Sophie Fritschi, Daniel E Speiser, Cyrill A Rentsch, David Gfeller, Patrice Jichlinski, Denise Nardelli-Haefliger, Camilla Jandus, Laurent Derré
Non-muscle-invasive bladder cancer (NMIBC) is a highly recurrent tumor despite intravesical immunotherapy instillation with the bacillus Calmette-Guérin (BCG) vaccine. In a prospective longitudinal study, we took advantage of BCG instillations, which increase local immune infiltration, to characterize immune cell populations in the urine of patients with NMIBC as a surrogate for the bladder tumor microenvironment. We observed an infiltration of neutrophils, T cells, monocytic myeloid-derived suppressor cells (M-MDSCs), and group 2 innate lymphoid cells (ILC2)...
June 26, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28649558/characterization-of-lepidopteran-specific-cry1-and-cry2-gene-harbouring-native-bacillus-thuringiensis-isolates-toxic-against-helicoverpa-armigera
#11
Showkat Ahmad Lone, Abdul Malik, Jasdeep Chatrath Padaria
Bacillus thuringiensis (Bt) based biopesticides are feasible alternatives to chemical pesticides. Here, we present the distribution of lepidopteran-specific cry1 and cry2 genes in native B. thuringiensis. Forty four out of 86 colonies were found to harbour crystals by phase contrast microscopy exhibiting a Bt index of 0.51. PCR analysis resulted in the amplification of cry1 in 24 and cry2 in 14 isolates. Twelve of the isolates showed presence of both cry1 and cry2, while 18 isolates did not show presence of either of the genes...
September 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28649508/isolation-of-shewanella-putrefaciens-in-an-elderly-man-with-subacute-intestinal-obstruction-appendicitis
#12
Arif Maqsood Ali, Muhammad Noorulamin, Shazia Arif
Shewanella is Gram-negative motile bacillus, non fermentative and facultative anaerobe. Its natural habitat is all forms of water and soil, but has also been isolated from fish, dairy products, oils, and carcasses. Often found with microflora of the marine environment. Bacterial infections with Shewanella spp. are rare. The exposure to the marine environment, sea and diary food are considered as a risk factor for Shewanella spp. infection. Clinical infections seen are otitis, soft tissue infection, bacteremia, ear infection, eye infection, infective arthritis, osteomyelitis, infective endocarditis and peritonitis...
2017: IDCases
https://www.readbyqxmd.com/read/28649412/surface-topology-affects-wetting-behavior-of-bacillus-subtilis-biofilms
#13
Moritz Werb, Carolina Falcón García, Nina C Bach, Stefan Grumbein, Stephan A Sieber, Madeleine Opitz, Oliver Lieleg
The colonization of surfaces by bacterial biofilms constitutes a huge problem in healthcare and industry. When attempting biofilm inactivation or removal, it is crucial to sufficiently wet the biofilm surface with antibacterial agents; however, certain biofilms efficiently resist wetting, and the origin of this behavior remains to date unclear. Here, we demonstrate that, depending on the growth medium used, the model bacterium Bacillus subtilis can form biofilm colonies with distinct surface properties: we find either hydrophilic or two variants of hydrophobic behavior...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28649409/bacillus-subtilis-utilizes-the-dna-damage-response-to-manage-multicellular-development
#14
Kevin Gozzi, Carly Ching, Srinand Paruthiyil, Yinjuan Zhao, Veronica Godoy-Carter, Yunrong Chai
Bacteria switch between free-living and a multicellular state, known as biofilms, in response to cellular and environmental cues. It is important to understand how these cues influence biofilm development as biofilms are not only ubiquitous in nature but are also causative agents of infectious diseases. It is often believed that any stress triggers biofilm formation as a means of bacterial protection. In this study, we propose a new mechanism for how cellular and environmental DNA damage may influence biofilm formation...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28649408/ser-thr-protein-kinase-prkc-mediated-regulation-of-groel-is-critical-for-biofilm-formation-in-bacillus-anthracis
#15
Gunjan Arora, Andaleeb Sajid, Richa Virmani, Anshika Singhal, C M Santosh Kumar, Neha Dhasmana, Tanya Khanna, Abhijit Maji, Richa Misra, Virginie Molle, Dörte Becher, Ulf Gerth, Shekhar C Mande, Yogendra Singh
PrkC is a conserved Ser/Thr protein kinase encoded in Bacillus anthracis genome. PrkC is shown to be important for B. anthracis pathogenesis, but little is known about its other functions and phosphorylated substrates. Systemic analyses indicate the compelling role of PrkC in phosphorylating multiple substrates, including the essential chaperone GroEL. Through mass spectrometry, we identified that PrkC phosphorylates GroEL on six threonine residues that are distributed in three canonical regions. Phosphorylation facilitates the oligomerization of GroEL to the physiologically active tetradecameric state and increases its affinity toward the co-chaperone GroES...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28649405/bacillus-subtilis-biofilm-development-in-the-presence-of-soil-clay-minerals-and-iron-oxides
#16
Wenting Ma, Donghai Peng, Sharon L Walker, Bin Cao, Chun-Hui Gao, Qiaoyun Huang, Peng Cai
Clay minerals and metal oxides, as important parts of the soil matrix, play crucial roles in the development of microbial communities. However, the mechanism underlying such a process, particularly on the formation of soil biofilm, remains poorly understood. Here, we investigated the effects of montmorillonite, kaolinite, and goethite on the biofilm formation of the representative soil bacteria Bacillus subtilis. The bacterial biofilm formation in goethite was found to be impaired in the initial 24 h but burst at 48 h in the liquid-air interface...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28648455/kinetic-modelling-and-meta-analysis-of-the-b-subtilis-siga-regulatory-network-during-spore-germination-and-outgrowth
#17
O Ramaniuk, M Černý, L Krásný, J Vohradsky
This study describes the meta-analysis and kinetic modelling of gene expression control by sigma factor SigA of Bacillus subtilis during germination and outgrowth based on microarray data from 14 time points. The analysis computationally models the direct interaction among SigA, SigA-controlled sigma factor genes (sigM, sigH, sigD, sigX), and their target genes. Of the >800 known genes in the SigA regulon, as extracted from databases, 311 genes were analysed, and 190 were confirmed by the kinetic model as being controlled by SigA...
June 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28648403/genetic-determinant-of-bacillus-pumilus-lipase-lethality-and-its-application-as-positive-selection-cloning-vector-in-escherichia-coli
#18
Nobalanda Betty Mabizela-Mokoena, Shonisani Wendy Limani, Ignatious Ncube, Lizelle Piater, Derek Litthauer, Mulalo Bethuel Nthangeni
Positive selection vectors carry genes that upon expression produce proteins that cause host cell deaths. Insertion of foreign DNA fragments within the ORF of the gene disrupts the lethal effect of the expressed protein. This study described the cloning of Family I.4 Bacillus pumilus lipase gene whose expressed protein is toxic and lethal to Escherichia coli JM109 (DE3) cells. The determinant of toxicity was identified through Error-prone PCR to be the nature of amino acid residue resident at position 28 of the mature lipase protein...
June 22, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28648290/milk-originated-bacillus-cereus-sensu-lato-strains-harbouring-bacillus-anthracis-like-plasmids-are-genetically-and-phenotypically-diverse
#19
Marek Bartoszewicz, Paulina Sylwia Marjańska
Bacillus cereus sensu lato is widely distributed in food products, including raw and processed milk. Plasmids often determine bacterial virulence and toxicity, but their role in the evolution of B. cereus sensu lato is only partly known. Here, we observed that nearly 8% of B. cereus sensu lato isolates were positive for pXO1-like plasmids and 12% for pXO2-like plasmids in raw and ultra-heat-treated (UHT) milk from one dairy plant. However, pXO1-like plasmids were significantly more frequent in raw milk, while pXO2-like plasmids were more frequent in processed milk...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28648288/characterization-of-bacillus-megaterium-bacillus-pumilus-and-paenibacillus-polymyxa-isolated-from-a-pinot-noir-wine-from-western-washington-state
#20
Nicolas H von Cosmos, Bruce A Watson, J K Fellman, D S Mattinson, Charles G Edwards
This report provides the first confirmed evidence of Bacillus-like bacteria present in a wine from Washington State. These bacteria were isolated from a 2013 Pinot noir wine whose aroma was sensorially described as being 'dirty' or 'pond scum.' Based on physiological traits and genetic sequencing, three bacterial isolates were identified as Bacillus megaterium (strain NHO-1), Bacillus pumilus (strain NHO-2), and Paenibacillus polymyxa (strain NHO-3). These bacteria grew in synthetic media of low pH (pH 3.5) while some survived ethanol concentrations up to 15% v/v...
October 2017: Food Microbiology
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