Marie-France Dorion, Diana Casas, Irina Shlaifer, Moein Yaqubi, Peter Fleming, Nathan Karpilovsky, Carol X-Q Chen, Michael Nicouleau, Valerio E C Piscopo, Emma J MacDougall, Aeshah Alluli, Taylor M Goldsmith, Alexandria Schneider, Samuel Dorion, Nathalia Aprahamian, Adam MacDonald, Rhalena A Thomas, Roy W R Dudley, Jeffrey A Hall, Edward A Fon, Jack P Antel, Jo Anne Stratton, Thomas M Durcan, Roberta La Piana, Luke M Healy
BACKGROUND: Induced pluripotent stem cell-derived microglia (iMGL) represent an excellent tool in studying microglial function in health and disease. Yet, since differentiation and survival of iMGL are highly reliant on colony-stimulating factor 1 receptor (CSF1R) signaling, it is difficult to use iMGL to study microglial dysfunction associated with pathogenic defects in CSF1R. METHODS: Serial modifications to an existing iMGL protocol were made, including but not limited to changes in growth factor combination to drive microglial differentiation, until successful derivation of microglia-like cells from an adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) patient carrying a c...
April 5, 2024: Molecular Neurodegeneration