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Foxp3, treg,

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https://www.readbyqxmd.com/read/29024606/role-of-the-tslp-dc-ox40l-pathway-in-asthma-pathogenesis-and-airway-inflammation-in-mice
#1
Shuang Feng, Li Zhang, Xu-Hua Bian, Ying Luo, Guang-Hui Qin, Rui-Ming Shi
This study aims to explore the effect of the TSLP/DC/OX40L pathway in asthma pathogenesis and airway inflammation in mice. Sixty-five male BALF/c mice were divided into the control, asthma, IgG + asthma (IgG, 500 g/500 l, intratracheal injection of 50 l each time), LY294002 (OX40L inhibitor) + asthma (intratracheal injection of 2 mg/kg LY294002), and anti-TSLP + asthma (intratracheal injection of 500 ug/500 l TSLP antibody, 50 l each time) groups. ELISA was applied to measure the serum levels of IgE, ovalbumin (OVA)-sIgE, interleukin-4 (IL-4), IL-5, IL-13 and interferon-γ (IFN-γ), flow cytometry was employed to detect Treg cells and dendritic cell (DC) and lymphopoiesis...
October 12, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29023682/short-term-cytokine-stimulation-reveals-regulatory-t-cells-with-down-regulated-foxp3-expression-in-human-peripheral-blood
#2
Paula Tabares, Susanne Berr, Daniela Langenhorst, Birgit Sawitzki, Ineke Ten Berge, Hans-Peter Tony, Thomas Hünig
The identification of regulatory T cells (Treg cells) in human peripheral blood is an important tool in diagnosis, research and therapeutic intervention. As compared to lymphoid tissues, the frequencies of circulating Treg cells identified as CD4(+) CD25(+) Foxp3(+) are, however, low. We here show that many of these cells remain undetected due to transient down regulation of Foxp3, which rapidly decays in the absence of cytokine-mediated STAT5 signals. Short-term incubation of PBMCs or isolated CD4(+) T-cells, but not of lymph node cells, with IL-2, -7, or -15 more than doubles the frequency of Foxp3(+) CD25(+) among CD4(+) T-cells detectable by flow cytometry...
October 11, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29023237/corneal-tissue-from-dry-eye-donors-leads-to-enhanced-graft-rejection
#3
Takenori Inomata, Jing Hua, Takeshi Nakao, Tina Shiang, Homer Chiang, Afsaneh Amouzegar, Reza Dana
PURPOSE: To assess the effect of dry eye disease (DED) in graft donors on dendritic cell (DC) maturation, host T-cell sensitization, and corneal allograft rejection. METHODS: Corneas of control (healthy donor) and DED mice (C57BL/6) were transplanted onto fully allogeneic naive BALB/c recipients (n = 10 mice/group). Long-term allograft survival was evaluated for 8 weeks. Corneas and draining lymph nodes (dLNs) were harvested at posttransplantation day 14 (n = 5 mice/group)...
October 10, 2017: Cornea
https://www.readbyqxmd.com/read/29020928/g-allele-at-924-a%C3%A2-%C3%A2-g-position-of-foxp3-gene-promoter-as-a-risk-factor-for-tuberculosis
#4
Elham Beiranvand, Saeid Abediankenari, Soghra Khani, Hamideh Mahmoodzadeh Hosseini, Sirous Zeinali, Behnoush Beiranvand, Mehdi Goudarzi, Sima Sadat Seyedjavadi
BACKGROUND: Forkhead box protein 3 (FoxP3) is an important factor for development and function of Regulatory T cells (Treg). Studies have found an association between common gene polymorphisms in FoxP3 and some infectious diseases. The aim of this study was to evaluate possible associations between two Single nucleotide polymorphisms (SNPs) in the promoter of the FoxP3 gene to susceptibility to tuberculosis (TB) and the alteration of Foxp3 gene expression. METHODS: The pattern distribution of genotype at two position, -3279 A > C (rs3761548) and -924 A > G (rs2232365) on the promoter of FoxP3 gene was evaluated using polymerase chain reaction-single specific primer (PCR-SSP) method in 183 tuberculosis patients and 183 healthy control...
October 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29019858/epicutaneous-tolerance-induction-to-a-bystander-antigen-abrogates-colitis-and-ileitis-in-mice
#5
David Dunkin, M Cecilia Berin, Lucie Mondoulet, Steven Tobar, Garabet Yeretssian, Leticia Tordesillas, Alina Iuga, Thibaut Larcher, Virginia Guillespie, Pierre-Henri Benhamou, Jean-Frederic Colombel, Hugh A Sampson
BACKGROUND: Although inflammatory bowel disease (IBD) is a failure in maintaining tolerance to the intestinal microbiota, few studies have investigated the use of immunologic tolerance as a treatment approach for IBD. We hypothesized that induction of immune tolerance at a distal site could suppress intestinal inflammation through a process of bystander regulation. METHODS: Epicutaneous tolerance was induced by topical application of ovalbumin (OVA) using a Viaskin patch for 48 hours...
October 9, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29018440/human-gut-symbiont-roseburia-hominis-promotes-and-regulates-innate-immunity
#6
Angela M Patterson, Imke E Mulder, Anthony J Travis, Annaig Lan, Nadine Cerf-Bensussan, Valerie Gaboriau-Routhiau, Karen Garden, Elizabeth Logan, Margaret I Delday, Alistair G P Coutts, Edouard Monnais, Vanessa C Ferraria, Ryo Inoue, George Grant, Rustam I Aminov
OBJECTIVE: Roseburia hominis is a flagellated gut anaerobic bacterium belonging to the Lachnospiraceae family within the Firmicutes phylum. A significant decrease of R. hominis colonization in the gut of ulcerative colitis patients has recently been demonstrated. In this work, we have investigated the mechanisms of R. hominis-host cross talk using both murine and in vitro models. DESIGN: The complete genome sequence of R. hominis A2-183 was determined. C3H/HeN germ-free mice were mono-colonized with R...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29018225/s1p1-deletion-differentially-affects-th17-and-regulatory-t-cells
#7
Ahmet Eken, Rebekka Duhen, Akhilesh K Singh, Mallory Fry, Jane H Buckner, Mariko Kita, Estelle Bettelli, Mohamed Oukka
Sphingosine-1 phosphate receptor 1 (S1P1) is critical for the egress of T and B cells out of lymphoid organs. Although S1P1 agonist fingolimod is currently used for the treatment of multiple sclerosis (MS) little is known how S1P1 signaling regulates Th17 and Treg cell homeostasis. To study the impact of S1P1 signaling on Th17 and Treg cell biology, we specifically deleted S1P1 in Th17 and Treg cells using IL-17A (Cre) and Foxp3 (Cre) mice, respectively. Deletion of S1P1 in Th17 cells conferred resistance to experimental autoimmune encephalomyelitis (EAE)...
October 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29016688/tlr2-tlr4-activation-induces-tregs-and-suppresses-intestinal-inflammation-caused-by-fusobacterium-nucleatum-in-vivo
#8
Yin-Ping Jia, Kun Wang, Zhu-Jun Zhang, Ya-Nan Tong, Dan Han, Chun-Yu Hu, Qian Li, Yang Xiang, Xu-Hu Mao, Bin Tang
Toll-like receptors (TLRs) 2 and 4 play critical roles in intestinal inflammation caused by Fusobacterium nucleatum (F. nucleatum) infection, but the role of TLR2/TLR4 in regulation of proinflammatory cytokines remains unknown. In this study, through microarray analysis and qRT-PCR, we showed that TLR2/TLR4 are involved in the F. nucleatum-induced inflammatory signaling pathway in Caco-2 cells, C57BL/6 mice and human clinical specimens. In TLR2-/- and TLR4-/- mice, F. nucleatum infection resulted in increased colonization of the bacteria and production of the proinflammatory cytokines IL-8, IL-1β and TNF-α...
2017: PloS One
https://www.readbyqxmd.com/read/28993609/transcription-factor-foxo1-is-essential-for-il-9-induction-in-t-helper-cells
#9
Sakshi Malik, Srikanth Sadhu, Srikanth Elesela, Ramendra Pati Pandey, Amanpreet Singh Chawla, Deepak Sharma, Lipsa Panda, Deepak Rathore, Balram Ghosh, Vineet Ahuja, Amit Awasthi
Interleukin 9 (IL-9)-producing helper T (Th9) cells have a crucial function in allergic inflammation, autoimmunity, immunity to extracellular pathogens and anti-tumor immune responses. In addition to Th9, Th2, Th17 and Foxp3(+) regulatory T (Treg) cells produce IL-9. A transcription factor that is critical for IL-9 induction in Th2, Th9 and Th17 cells has not been identified. Here we show that the forkhead family transcription factor Foxo1 is required for IL-9 induction in Th9 and Th17 cells. We further show that inhibition of AKT enhances IL-9 induction in Th9 cells while it reciprocally regulates IL-9 and IL-17 in Th17 cells via Foxo1...
October 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28993346/abatacept-in-the-treatment-of-adult-dermatomyositis-and-polymyositis-a-randomised-phase-iib-treatment-delayed-start-trial
#10
Anna Tjärnlund, Quan Tang, Cecilia Wick, Maryam Dastmalchi, Herman Mann, Jana Tomasová Studýnková, Radka Chura, Nicola J Gullick, Rosaria Salerno, Johan Rönnelid, Helene Alexanderson, Eva Lindroos, Rohit Aggarwal, Patrick Gordon, Jiri Vencovsky, Ingrid E Lundberg
OBJECTIVES: To study the effects of abatacept on disease activity and on muscle biopsy features of adult patients with dermatomyositis (DM) or polymyositis (PM). METHODS: Twenty patients with DM (n=9) or PM (n=11) with refractory disease were enrolled in a randomised treatment delayed-start trial to receive either immediate active treatment with intravenous abatacept or a 3 month delayed-start. The primary endpoint was number of responders, defined by the International Myositis Assessment and Clinical Studies Group definition of improvement (DOI), after 6 months of treatment...
October 9, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28993118/th17-treg-ratio-in-serum-predicts-onset-of-postoperative-atrial-fibrillation-after-off-pump-coronary-artery-bypass-graft-surgery
#11
Yi He, Xi Chen, Xuejun Guo, Hang Yin, Nan Ma, Min Tang, Hao Liu, Ju Mei
BACKGROUND: The aim of this study is to identify whether the balance between T helper 17 (Th17) cells and CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cells could predict the postoperative atrial fibrillation (POAF) after coronary artery bypass graft surgery (CABG). METHODS: We enrolled 88 patients from Xinhua Hospital who received off-pump CABG (OPCABG) surgery. The baseline characteristics of patients were recorded. The preoperative variables C-reactive protein (CRP) level, left atrial (LA) volume, EuroSCORE I score, CHADS2 score, and CHA2DS2-VASc score were calculated at enrolment...
September 23, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28988715/self-transducible-bimodal-pdx1-foxp3-protein-lifts-insulin-secretion-and-curbs-autoimmunity-boosting-tregs-in-type-1-diabetic-mice
#12
Christina Amatya, Ilian A Radichev, Jacob Ellefson, Mark Williams, Alexei Y Savinov
Type 1 diabetes (T1D) is characterized by massive destruction of insulin-producing β cells by autoreactive T lymphocytes, arising via defective immune tolerance. Therefore, effective anti-T1D therapeutics should combine autoimmunity-preventing and insulin production-restoring properties. We constructed a cell-permeable PDX1-FOXP3-TAT fusion protein (FP) composed of two transcription factors: forkhead box P3 (FOXP3), the master regulator of differentiation and functioning of self-tolerance-promoting Tregs, and pancreatic duodenal homeobox-1 (PDX1), the crucial factor supporting β cell development and maintenance...
September 7, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28983871/human-amylin-induces-cd4-foxp3-regulatory-t-cells-in-the-protection-from-autoimmune-diabetes
#13
Xiao-Xi Zhang, Yong-Chao Qiao, Wan Li, Xia Zou, Yin-Ling Chen, Jian Shen, Qin-Yuan Liao, Qiu-Jin Zhang, Lan He, Hai-Lu Zhao
Autoimmune diabetes is a disorder of immune homeostasis that leads to targeted insulin-secreting islet β cell destruction characterized by insulitis. Human amylin (hA) is an important neuroendocrine hormone co-secreted with insulin by pancreatic β cells. Here, we report hA immune-modulatory action through inducing regulatory T cells. We ex vivo-treated human peripheral blood mononuclear cells (hPBMCs) with hA for 24 h and counted CD4+Foxp3+ regulatory T cells (Treg) using flow cytometry. Diabetic status was monitored and splenic Treg were measured in non-obese diabetic (NOD) male mice...
October 5, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28983592/amygdalin-ameliorates-the-progression-of-atherosclerosis-in-ldl-receptor%C3%A2-deficient-mice
#14
Jianzhen Lv, Wen Xiong, Tiantian Lei, Hailian Wang, Minghan Sun, Erwei Hao, Zhiping Wang, Xiaoqi Huang, Shaoping Deng, Jiagang Deng, Yi Wang
Previous studies have demonstrated that regulatory T cells (Tregs) are pivotal in the regulation of T cell‑mediated immune responses in atherosclerosis, a chronic autoimmune‑like disease. In the authors' previous studies, it was demonstrated that amygdalin ameliorated atherosclerosis by the regulation of Tregs in apolipoprotein E‑deficient (ApoE‑/‑) mice. Therefore, the aim of the present study was to investigate the therapeutic effect of amygdalin on low‑density lipoprotein (LDL) receptor deficient (LDLR‑/‑) mice, and to examine its immune regulatory function by the stimulation of Tregs...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28983404/functional-heterogeneity-of-gut-resident-regulatory-t-cells
#15
REVIEW
Maik Luu, Ulrich Steinhoff, Alexander Visekruna
Regulatory T cells (Treg cells) have a central role in the maintenance of intestinal homeostasis by restraining inappropriate immune responses in the healthy gut. Although distinct intestinal immune cell populations have been described to exhibit regulatory activity, several genetic and functional studies provided a strong evidence for a pivotal role of forkhead box P3 (Foxp3)(+)CD4(+) Treg cells in prevention of dysregulated mucosal immune reactions and development of chronic immunological disorders such as celiac disease, food allergies and inflammatory bowel disease...
September 2017: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/28983402/regulatory-t-cell-heterogeneity-and-the-cancer-immune-response
#16
REVIEW
Kirsten A Ward-Hartstonge, Roslyn A Kemp
The frequency of circulating or tumour-infiltrating regulatory T cells (Tregs) has been associated with poor patient survival in many cancers including breast, melanoma and lung. It has been hypothesised that Tregs impact the anti-tumour function of effector T cells, resulting in worse outcomes for patients. However, high infiltrates of Tregs have been associated with a positive outcome of patients in a minority of cancers including colorectal, bladder and oesophageal. In addition, many studies have shown no impact of Tregs in patient outcome...
September 2017: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/28982868/peripheral-tr1-and-foxp3-treg-as-markers-of-recurrent-malignancies-in-patients-with-hepato-biliary-pancreatic-cancers
#17
Tetsuya Ikemoto, Mitsuo Shimada, Daichi Ishikawa, Yoichiro Kawashita, Hiroki Teraoku, Masato Yoshikawa, Shinichiro Yamada, Y U Saito, Yuji Morine, Satoru Imura
BACKGROUND/AIM: Recently CD4(+)CD49b(+) LAG3(+)regulatory T (Tr1) cells are reported to be IL-10 driven, have strong regulatory activities. Thus, this study aimed to investigate whether Treg and Tr1 cells participate in immunological status against cancer. PATIENTS AND METHODS: Peripheral blood was withdrawn from patients (n=78), and healthy volunteers as controls (n=23). The peripheral blood mononuclear cells were subjected to FACScan analysis after labeling with anti-CD4, -CD25, - Foxp3, -CD49b and -LAG3 antibodies...
October 2017: Anticancer Research
https://www.readbyqxmd.com/read/28979672/engrafted-peripheral-blood-derived-mesenchymal-stem-cells-promote-locomotive-recovery-in-adult-rats-after-spinal-cord-injury
#18
Qiang Fu, Yi Liu, Xiu Liu, Qian Zhang, Long Chen, Jiachen Peng, Jun Ao, Yuwan Li, Shengmin Wang, Gongyu Song, Limei Yu, Jinwei Liu, Tao Zhang
Spinal cord injury (SCI) is a severe trauma of central nervous system (CNS). Numerous stem cells have been applied for SCI therapy. Peripheral blood-derived mesenchymal stem cells (PBMSCs) have captured researchers' attention by virtue of pluripotency and effectiveness. However, little work has been performed on whether PBMSCs play roles and what role, if any, in the lesion microenvironment. Through the investigation of the differentiation, neuroprotection and immunoloregulation of engrafted PBMSCs, we found that the expression of glial fibrillary acidic protein (GFAP) was inhibited...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28978795/dock8-regulates-fitness-and-function-of-regulatory-t-cells-through-modulation-of-il-2-signaling
#19
Akhilesh K Singh, Ahmet Eken, David Hagin, Khushbu Komal, Gauri Bhise, Azima Shaji, Tanvi Arkatkar, Shaun W Jackson, Estelle Bettelli, Troy R Torgerson, Mohamed Oukka
Foxp3+ Tregs possess potent immunosuppressive activity, which is critical for maintaining immune homeostasis and self-tolerance. Defects in Treg development or function result in inadvertent immune activation and autoimmunity. Despite recent advances in Treg biology, we still do not completely understand the molecular and cellular mechanisms governing the development and suppressive function of these cells. Here, we have demonstrated an essential role of the dedicator of cytokinesis 8 (DOCK8), guanine nucleotide exchange factors required for cytoskeleton rearrangement, cell migration, and immune cell survival in controlling Treg fitness and their function...
October 5, 2017: JCI Insight
https://www.readbyqxmd.com/read/28978474/precocious-interleukin-21-expression-in-naive-mice-identifies-a-natural-helper-cell-population-in-autoimmune-disease
#20
Elisabeth A Marnik, Xulong Wang, Thomas J Sproule, Giljun Park, Gregory J Christianson, Sarah Kate Lane-Reticker, Shweta Jain, Theodore Duffy, Hongsheng Wang, Gregory W Carter, Herbert C Morse, Derry C Roopenian
Interleukin 21 (IL-21) plays key roles in humoral immunity and autoimmune diseases. It is known to function in mature CD4(+) T follicular B cell helper (TFH) cells, but its potential involvement in early T cell ontogeny is unclear. Here, we find that a significant population of newly activated thymic and peripheral CD4(+) T cells functionally expresses IL-21 soon after birth. This naturally occurring population, termed natural (n)TH21 cells, exhibits considerable similarity to mature TFH cells. nTH21 cells originating and activated in the thymus are strictly dependent on autoimmune regulator (AIRE) and express high levels of NUR77, consistent with a bias toward self-reactivity...
October 3, 2017: Cell Reports
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