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Zhenle Yang, Hongying Ji, Dantong Liu
Cyperus esculentus is unique in that it can accumulate rich oil in tuber. However, the underlying mechanism of tuber oil biosynthesis is still unclear. Our transcriptional analyses of the pathways from pyruvate production up to triacylglycerol (TAG) accumulation in tuber revealed many distinct species-specific lipid expression patterns from oil seeds and fruits, indicating that in C. esculentus tuber, (1) carbon flux from sucrose toward plastid pyruvate could be produced mostly through cytosolic glycolytic pathway, (2) acetyl-CoA synthetase might be an important contributor to acetyl-CoA formation for plastid fatty acid biosynthesis, (3) expression pattern for stearoyl-ACP desaturase was associated with high oleic acid composition, (4) it was most likely that endoplasmic reticulum-associated acyl-CoA synthetase played a significant role in the export of fatty acids between the plastid and ER, (5) lipid phosphate phosphatase (LPP)-δ was most probably related to the formation of DAG pool in Kennedy pathway, (6) diacylglyceroltransacylase 2 (DGAT2) and phospholipid:diacylglycerolacyltransferase 1 (PDAT1) might play crucial roles in tuber oil biosynthesis...
October 13, 2016: Plant & Cell Physiology
Yangyong Lv, Shuaibing Zhang, Jinshui Wang, Yuansen Hu
Wheat (Triticum aestivum L.) is an important crop worldwide. The physiological deterioration of seeds during storage and seed priming is closely associated with germination, and thus contributes to plant growth and subsequent grain yields. In this study, wheat seeds during different stages of artificial ageing (45°C; 50% relative humidity; 98%, 50%, 20%, and 1% Germination rates) and priming (hydro-priming treatment) were subjected to proteomics analysis through a proteomic approach based on the isobaric tandem mass tag labeling...
2016: PloS One
Abdulsamie Hanano, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Elizabeth Blee, Denis J Murphy
Contamination of aquatic environments with dioxins, the most toxic group of persistent organic pollutants (POPs), is a major ecological issue. Dioxins are highly lipophilic and bioaccumulate in fatty tissues of marine organisms used for seafood where they constitute a potential risk for human health. Lipid droplets (LDs) purified from date palm, Phoenix dactylifera, seeds were characterized and their capacity to extract dioxins from aquatic systems was assessed. The bioaffinity of date palm LDs toward 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins was determined...
2016: Frontiers in Plant Science
Qing Liu, Qigao Guo, Sehrish Akbar, Yao Zhi, Anna El Tahchy, Madeline Mitchell, Zhongyi Li, Pushkar Shrestha, Thomas Vanhercke, Jean-Philippe Ral, Guolu Liang, Ming-Bo Wang, Rosemary White, Philip Larkin, Surinder Singh, James Petrie
Potato tuber is a high yielding food crop known for its high levels of starch accumulation but only negligible levels of triacylglycerol (TAG). In this study we evaluated the potential for lipid production in potato tubers by simultaneously introducing three transgenes, including WRINKLED 1 (WRI1), DIACYLGLYCEROL ACYLTRANSFERASE 1 (DGAT1) and OLEOSIN under the transcriptional control of tuber-specific (patatin) and constitutive (CaMV-35S) promoters. This coordinated metabolic engineering approach resulted in over a 100- fold increase of TAG accumulation to levels up to 3...
June 16, 2016: Plant Biotechnology Journal
Chen Gao, Kevin B Vargo, Daniel A Hammer
Targeting nanoparticles for drug delivery has great potential for improving efficacy and reducing side effects from systemic toxicity. New developments in the assembly of materials afford the opportunity to expose cryptic targeting domains in tissue-specific microenvironments in which certain proteases are expressed. Here, recombinant proteins are designed to combine the responsiveness to environmental proteases with specific targeting. Materials made recombinantly allow complete control over amino acid sequence, in which each molecule is identically functionalized...
September 2016: Macromolecular Bioscience
Min Liu, Shenghui Chu, Jun Ai, Haiyan Li, Zhiyu Chen, Sisi Huang, Chao Jiang, Xiaokun Li
OBJECTIVE: To determine an efficient expression strategy in Arabidopsis thaliana and to determine whether a dimeric oleosin fusion approach could achieve keratinocyte growth factor-2 (KGF2) expression and bioactivity. RESULTS: Higher recombinant protein accumulation was observed in the two dimeric oleosin constructs than in a single-oleosin-fused protein (O::KGR2) or KGF2 control. Highest expression was in O-O::KGF2-transgenic seeds. MTT assay in FGFR2 III b-BaF3 cells revealed comparable levels of bioactivity due to O-O::KGF2 and O::KGR2, whereas O::KGF2-O had no effect on FGFR2 III b-BaF3 opithelial cell growth...
September 2016: Biotechnology Letters
Ming-Der Huang, Anthony H C Huang
Subcellular lipid droplets (LDs) in diverse plant cells and species are coated with stabilizing oleosins of at least five phylogenic lineages and perform different functions. We examined two types of inadequately studied LDs for coated oleosins and their characteristics. The epidermis but not mesophyll of leaves of vanilla (Vanilla planifolia) and most other Asparagales species contained solitary and clustered LDs (<0.5 μm), some previously studied by electron microscopy and speculated to be for cuticle formation...
July 2016: Plant Physiology
Li Zhang, Shi-Bo Wang, Qi-Gang Li, Jian Song, Yu-Qi Hao, Ling Zhou, Huan-Quan Zheng, Jim M Dunwell, Yuan-Ming Zhang
Seed oils provide a renewable source of food, biofuel and industrial raw materials that is important for humans. Although many genes and pathways for acyl-lipid metabolism have been identified, little is known about whether there is a specific mechanism for high-oil content in high-oil plants. Based on the distinct differences in seed oil content between four high-oil dicots (20~50%) and three low-oil grasses (<3%), comparative genome, transcriptome and differential expression analyses were used to investigate this mechanism...
2016: PloS One
Philip E Johnson, Rebekah L Sayers, Lee A Gethings, Anuradha Balasundaram, Justin T Marsh, James I Langridge, E N Clare Mills
Profiling allergens in complex food ingredients used in oral food challenges and immunotherapy is crucial for regulatory acceptance. Mass spectrometry based analysis employing data-independent acquisition coupled with ion mobility mass spectrometry-mass spectrometry (DIA-IM-MS) was used to investigate the allergen composition of raw peanuts and roasted peanut flour ingredients used in challenge meals. This comprehensive qualitative and quantitative analysis using label-free approaches identified and quantified 123 unique protein accessions...
June 7, 2016: Analytical Chemistry
Sweta Kumari, Lucia Joseph Memba, Anil Dahuja, T Vinutha, Supradip Saha, Archana Sachdev
Defatting soybean by sophisticated oil extraction method utilising supercritical CO2 resulted in a significant decrease in the residual phospholipids (PLs) compared with soymeal obtained by conventional cold percolation method utilising hexane as the extraction solvent. Interestingly, the levels of residual PLs showed a proportionate relationship with thiobarbituric acid (TBA) number, an indicator of lipid peroxidation responsible for off-flavour generation. Furthermore, two oleosins (18 and 24 kDa) were isolated from the oil bodies extracted from soybean seeds and positively characterised for phospholipase A2 (PLA2) activity, suggesting their plausible involvement in off-flavour generation in soymeal...
August 15, 2016: Food Chemistry
Malgorzata Teodorowicz, Rozine J Terlouw, Ad Jansen, Huub F J Savelkoul, Janneke Ruinemans-Koerts
BACKGROUND: Sesame seed is an allergen of growing importance worldwide. However, knowledge of the clinically relevant sesame allergen and its cross-reactivity with homologous allergens is limited. The aim of this study was the immunological characterization of Dutch sesame seed-allergic patients and evaluation of cross-reactivity between sesame seed, tree nut and pollen allergens using different sources of allergen extracts. METHODS: Six patients with a medical history of sesame seed allergy were included, i...
2016: International Archives of Allergy and Immunology
Buntora Pasaribu, Tse-yu Chung, Chii-Shiarng Chen, Pei-Luen Jiang, Jason T C Tzen
Three classes of integral proteins termed oleosin, caleosin and steroleosin have been identified in seed oil bodies of diverse angiosperm species. Recently, two oleosin isoforms and one caleosin were identified in megagametophyte oil bodies of pine (Pinus massoniana), a representative gymnosperm species. In this study, a putative steroleosin of approximately 41 kDa was observed in isolated oil bodies of pine megagametophytes, and its corresponding cDNA fragment was obtained by PCR cloning and further confirmed by mass spectrometric analysis...
April 2016: Plant Physiology and Biochemistry: PPB
Jianqin Huang, Tong Zhang, Qixiang Zhang, Ming Chen, Zhengjia Wang, Bingsong Zheng, Guohua Xia, Xianyou Yang, Chunying Huang, Youjun Huang
BACKGROUND: Hickory (Carya cathayensis Sarg.) accumulates more than 70% oil and 90% unsaturated fatty acids with considerably high oleic acid in its mature embryo. The concurrent global trancriptomic and lipidomic analyses provided a framework for better understanding of glycerolipid biosynthesis and metabolism in the hickory nut. RESULTS: The synthetical regulation of numerous leading lipid-related genes harmonized with the oil accumulation and fatty acid conversion in embryo development...
2016: BMC Genomics
Luping Zhao, Yeming Chen, Yajing Chen, Xiangzhen Kong, Yufei Hua
Plant seeds are used to extract oil bodies for diverse applications, but oil bodies extracted at different pH values exhibit different properties. Jicama, sunflower, peanut, castor bean, rapeseed, and sesame were selected to examine the effects of pH (6.5-11.0) on the protein components of oil bodies and the oleosin hydrolysis in pH 6.5-extracted oil bodies. In addition to oleosins, many extrinsic proteins (globulins, 2S albumin, and enzymes) were present in pH 6.5-extracted oil bodies. Globulins were mostly removed at pH 8...
June 1, 2016: Food Chemistry
Anita Thakur, Satish C Bhatla
A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20-30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20-30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes...
2015: Plant Signaling & Behavior
Laura Montesinos, Mireia Bundó, Esther Izquierdo, Sonia Campo, Esther Badosa, Michel Rossignol, Emilio Montesinos, Blanca San Segundo, María Coca
Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production and purification costs. Here, we report the efficient production of this bioactive peptide in rice bran using the rice oleosin 18 as a carrier protein. High cecropin A levels were reached in rice seeds driving the expression of the chimeric gene by the strong embryo-specific oleosin 18 own promoter, and targeting the peptide to the oil body organelle as an oleosin 18-cecropin A fusion protein...
2016: PloS One
Cory L Nykiforuk
Fundamentally, oil bodies are discrete storage organelles found in oilseeds, comprising a hydrophobic triacylglycerol core surrounded by a half-unit phospholipid membrane and an outer shell of specialized proteins known as oleosins. Oil bodies possess a number of attributes that were exploited by SemBioSys Genetics to isolate highly enriched fractions of oil bodies through liquid-liquid phase separation for a number of commercial applications. The current chapter provides a general guide for the isolation of oil bodies from Arabidopsis and/or safflower seed, from which protocols can be refined for different oilseed sources...
2016: Methods in Molecular Biology
Zunhao Yan, Luping Zhao, Xiangzhen Kong, Yufei Hua, Yeming Chen
In unheated soymilk, extrinsic proteins are bound to intact oil bodies coated by one monolayer of phospholipids and oil body intrinsic oleosins. In this study, effects of heating (70-100°C; 0-30min) on particle size and bound proteins of oil bodies were examined in suspension (oil bodies from unheated soymilk into deionized water) and soymilk. Mass ratio of extrinsic proteins/oleosins of oil bodies in unheated suspension and soymilk was respectively 1.1 and 2.5. By heating, extrinsic proteins released from oil bodies with different rates, and Z-average size of oil bodies increased in the beginning; afterwards, residual extrinsic proteins (extrinsic proteins/oleosins: 0...
March 1, 2016: Food Chemistry
Jian Huang, Jing Yang, Lili Guan, Shanyong Yi, Linna Du, Haishan Tian, Yongxin Guo, Feng Zhai, Zhen Lu, Haiyan Li, Xiaokun Li, Chao Jiang
Fibroblast growth factor 10 (FGF10) is a member of the FGF superfamily. It exhibits diverse biological functions, and is extensively used for fundamental research and clinical applications involving hair growth, tissue repair, and burn wounds. Oil bodies, obtained from oil seeds, have been exploited for a variety of biotechnology applications. The use of oil bodies reduces purification steps and costs associated with the production of heterogonous proteins. Here, recombinant human FGF10 (rhFGF10) was expressed in safflower (Carthamus tinctorius L...
September 15, 2015: Protein Expression and Purification
J Lacomba Montoro, J Doménech Witek, F Pineda de la Losa, V Jover Cerdà
No abstract text is available yet for this article.
2015: Journal of Investigational Allergology & Clinical Immunology
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