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Nora Bloise, Loredana Petecchia, Gabriele Ceccarelli, Lorenzo Fassina, Cesare Usai, Federico Bertoglio, Martina Balli, Massimo Vassalli, Maria Gabriella Cusella De Angelis, Paola Gavazzo, Marcello Imbriani, Livia Visai
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated...
2018: PloS One
Hui-Yi Hsiao, Chin-Yu Yang, Jia-Wei Liu, Eric M Brey, Ming-Huei Cheng
Periosteal osteogenic capacity can be exploited to enhance bone formation in the fields of tissue engineering and regenerative medicine. Despite this importance, there have been no studies examining the composition, structure and osteogenic capacity of periostea from different bone sources. In this study, structure and osteogenic factor content were compared among periostea from calvarial, rib, femoral and tibial bone, in which the native bones of these four regions were harvested and subjected to histological analysis...
June 14, 2018: Tissue Engineering. Part A
Tianxiang Geng, Xi Chen, Mengxue Zheng, Haochen Yu, Shuai Zhang, Shouxuan Sun, Haohui Guo, Qunhua Jin
Aseptic loosening and menopause‑induced osteoporosis are caused by an imbalance between bone formation and osteolysis. With an aging population, the probability of simultaneous occurrence of such conditions in an elderly individual is increasing. Strontium ranelate (SR) is an anti‑osteoporosis drug that promotes bone formation and inhibits osteolysis. The present study compared the effects of SR with those of the traditional anti‑osteoporosis drug alendronate (ALN) using an ovariectomized mouse model of osteolysis...
June 5, 2018: Molecular Medicine Reports
Renata Suellen Galvão da Silva-Costa, Andressa Eveline de Lima Ribeiro, Isauremi Vieira de Assunção, Raimundo Fernandes de Araújo Júnior, Aurigena Antunes de Araújo, Gerlane Coelho Bernardo Guerra, Boniek Castillo Dutra Borges
OBJECTIVES: To study the intensity of inflammatory infiltrate and production of interleukin-1β (ll-1β), tumor necrosis factor-β (TNF-β), fibroblast growth factor-2 (FGF-2), glutathione peroxidase (GPX), and osteocalcin in response to in-office tooth bleaching in rats. MATERIAL AND METHODS: Twenty male Wistar rats were randomized into four groups (n=5) according to the received treatment (tooth bleaching or no treatment - control) and the period of euthanasia after treatment (24 h or 10 days)...
June 11, 2018: Journal of Applied Oral Science: Revista FOB
Jaqueline Suemi Hassumi, Gabriel Mulinari-Santos, André Luis da Silva Fabris, Ricardo Garcia Mureb Jacob, Alaíde Gonçalves, Ana Cláudia Rossi, Alexandre Rodrigues Freire, Leonardo Pérez Faverani, Roberta Okamoto
INTRODUCTION: Alveolar bone healing after upper incisor extraction in rats is a classical model of preclinical studies. The underlying morphometric, cellular and molecular mechanism, however, remains imprecise in a unique study. OBJECTIVES: The aim of this study was therefore to characterize the alveolar bone healing after upper incisor extraction in rats by micro computed tomographic (Micro-CT), immunohistochemical and real-time polymerase chain reaction (RT-PCR) analysis...
June 11, 2018: Journal of Applied Oral Science: Revista FOB
Maria B Asparuhova, Jordi Caballé-Serrano, Daniel Buser, Vivianne Chappuis
Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral (DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells...
June 12, 2018: International Journal of Oral Science
Shuangshuang Wang, Siwang Hu, Jian Wang, Yahui Liu, Ruochi Zhao, Maoqing Tong, Hanbin Cui, Nan Wu, Xiaomin Chen
BACKGROUND: Arterial calcification is associated with cardiovascular disease as a complication of advanced atherosclerosis and is a significant contributor to cardiovascular morbidity and mortality. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important role in arterial calcification and is characterized by cellular necrosis, inflammation, and lipoprotein and phospholipid complexes, especially in atherosclerotic calcification. The conditioned medium from bone marrow-derived mesenchymal stem cells (MSC-CM) is well known as a rich source of autologous cytokines and is universally used for tissue regeneration in current clinical medicine...
June 13, 2018: Stem Cell Research & Therapy
Firuzan Firat Ozer, Selcuk Dagdelen, Tomris Erbas
The objective of this study was to investigate the effect of hyperprolactinemia and high levels of insulin-like growth factor-I (IGF-I) on bone resorption and their relation with receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) in patients with prolactinoma and acromegaly. Thirty-one patients with acromegaly, 28 patients with prolactinoma, and 33 healthy individuals were included in the study. Serum concentrations of RANKL, OPG, bone alkaline phosphatase (bone ALP), osteocalcin (OC), C-terminal telopeptide of type 1 collagen (CTX), procollagen type 1 N-terminal propeptide (P1NP) and urine deoxypyridinoline (DPD) levels were detected and bone mineral density (BMD) was measured...
June 12, 2018: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
Hao Geng, Jiajun Qiu, Hongqin Zhu, Xuanyong Liu
Nitrogen doped graphene quantum dots (N-GQDs) were synthesized to explore and extend their potential applications in biomedical field. The hemocompatibility and cytotoxity of the obtained N-GQDs were primarily assessed at concentrations ranging from 10 to 100 μg/ml. From the results, it was found that the proliferation of rat Bone Mesenchymal Stem Cells (rBMSCs) was depressed to a certain extent after incubating with the high concentration (100 μg/ml) of N-GQDs. The nanoscale size and superior dispersibility endow N-GQDs with good cell permeability...
June 11, 2018: Journal of Materials Science. Materials in Medicine
Stuti Gupta, Shivjot Chhina, Sachit Anand Arora
The objective of the study was to conduct a systematic review of the literature so as to evaluate and summarize the diagnostic and prognostic potential of GCF. Included studies were systematically analyzed based on PRISMA (Preferred Reporting Items For Systematic Reviews and Meta Analyses) and studies were identified based on the-PICO (Glossary of evidence based terms 2007): 1)Patients with chronic periodontitis.2)Intervention- NSPT (Non-SurgicalPeriodontal therapy); NSPT + Chemotherapeutics.3)Comparison between treated v/s non treated sites...
May 2018: Journal of Oral Biology and Craniofacial Research
Hua Han, ZhengQing Li, Na Qu, Si Chen, PeiLiang Dong
Sambucus williamsii Hance has been used in fractures for thousands of years, but research on its active components, such as morroniside, until now had not been carried out. In this study, morroniside was taken as the leading compound, and fourteen derivatives were synthesized. The promotion of osteoblast proliferation effect of the derivatives was evaluated on MC3T3-E1 cells. Five derivatives ( 2 , 3 , 4 , 5 , and 14 ) showed a good proliferation effect on MC3T3-E1 cells, and their promoted expression effects on OC (Osteocalcin) and ALP (Alkaline phosphatase) in MC3T3-E1 cells were measured...
June 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Igor A Khlusov, Yuri Dekhtyar, Yurii P Sharkeev, Vladimir F Pichugin, Marina Y Khlusova, Nataliya Polyaka, Fedor Tyulkin, Viktorija Vendinya, Elena V Legostaeva, Larisa S Litvinova, Valeria V Shupletsova, Olga G Khaziakhmatova, Kristina A Yurova, Konstantin A Prosolov
Mesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior...
June 9, 2018: Materials
Amir Aviram, Biana Shtaif, Galia Gat-Yablonski, Yariv Yogev
OBJECTIVE: To evaluate the relationship between adipocytokines and glycemic control. STUDY DESIGN: Prospective observational trial of gestations with gestational diabetes mellitus (GDM). Fasting glucose (FG), insulin, adiponectin, Leptin, chemerin, Retinol Binding Protein 4 (RBP-4), osteocalcin and resistin were measured. HomeOstasis model assessment of insulin resistance (HOMA-IR) and QUantitative insulin sensitivity ChecK Index (QUICKI) were calculated. Women who required medications for glycemic control were compared to women using nutritional therapy only...
June 10, 2018: Journal of Maternal-fetal & Neonatal Medicine
İsmail Onur Sahin, Ceren Gokmenoglu, Cankat Kara
OBJECTIVE: The purpose of this study was to assess the effect of concentrated growth factor (CGF) on osteoblast cell proliferation and differentiation. METHODS: SaOS-2 osteoblast-like cells were cultured on titanium discs. The disc surfaces in the test group were treated with CGF serum, and those in the control group were left untreated. Cell counts, cell proliferation, and osteocalcin (OCN) levels were evaluated on days 1 and 3, and the alkaline phosphatase (ALP) activity was assessed on days 3, 7, and 14...
June 7, 2018: Journal of Stomatology, Oral and Maxillofacial Surgery
Young Hee Nam, Colleen M Brensinger, Warren B Bilker, Charles E Leonard, Xu Han, Sean Hennessy
Prior research suggests that warfarin, when given concomitantly with some sulfonylureas, may increase the risk of serious hypoglycemia. However, the clinical significance remains unclear. We examined rate ratios (RRs) for the association between serious hypoglycemia and concomitant use of warfarin with either sulfonylureas or metformin using a self-controlled case series design and US Medicaid claims (supplemented with Medicare claims) from 1999-2011. Across all risk windows combined, warfarin was associated with an elevated rate of serious hypoglycemia when given concomitantly with glimepiride (RR:1...
June 9, 2018: Clinical Pharmacology and Therapeutics
Shuli Fan, Xiang Gao, Peng Chen, Xu Li
Myricetin has been reported to promote osteogenic differentiation and inhibit osteoclastogenesis. The aim of this study was to investigate the potential effects of myricetin on glucocorticoid-induced osteoporosis (GIOP) both in vivo and in vitro. Male Sprague-Dawley (SD) rats were given dexamethasone (DEX, 0.1 mg/kg, s.c.) once daily and myricetin (1 mg/kg or 2.5 mg/kg, i.p.) once every other day for a total of five weeks. Body weight was recorded once a week. Bone mineral density (BMD), the activities or levels of bone turnover markers, and histological changes were assessed...
June 5, 2018: Life Sciences
Janusz Popko, Michał Karpiński, Sylwia Chojnowska, Katarzyna Maresz, Robert Milewski, Vladimir Badmaev, Leon J Schurgers
OBJECTIVE: In the past decades, an increased interest in the roles of vitamin D and K has become evident, in particular in relation to bone health and prevention of bone fractures. The aim of the current study was to evaluate vitamin D and K status in children with low-energy fractures and in children without fractures. METHODS: The study group of 20 children (14 boys, 6 girls) aged 5 to 15 years old, with radiologically confirmed low-energy fractures was compared with the control group of 19 healthy children (9 boys, 10 girls), aged 7 to 17 years old, without fractures...
June 6, 2018: Nutrients
Verónica Ávila-Rubio, Beatriz García-Fontana, Cristina Novo-Rodríguez, Jesús Cantero-Hinojosa, Rebeca Reyes-García, Manuel Muñoz-Torres
BACKGROUND: Postmenopausal osteoporosis (PMO) is associated with other comorbidities such as impaired glucose homeostasis and cardiovascular disease. Vitamin D insufficiency is highly prevalent and may be a common link between these disorders. However, the relationship between circulating 25-hydroxyvitamin D [25(OH)D] and insulin resistance in women with PMO has not been well evaluated. The aim of this study was to assess the relationship between circulating levels of 25(OH)D and parameters of glucose homeostasis in a cohort of women with PMO to establish a serum concentration threshold of 25(OH)D for improved glycemic parameters...
June 6, 2018: Journal of Women's Health
Han-Bing Song, Ying Jiang, Jia-Xing Liu, Ge-Qiang Wang, Da-Peng Zhang, Yi-Chang Jiang, Shu-Jun Ren, Hong-Peng Liu, Xue-Yan Jiang
A capsule of Qili Jiegu, a traditional Chinese medicine with numerous biological activities, may exert a protective eff ;ect against postmenopausal bone loss. However, it remains unclear whether Qili Jiegu-containing serum regulates the osteogenic diff ;erentiation of bone marrow stromal cells (BMSCs) in vitro. In this study, BMSCs were treated with medium and Qili Jiegu-containing serum over a 14-day period. We found that Qili Jiegu-containing serum promoted the BMSC proliferation and alkaline phosphatase (ALP) activities, as well as stimulated the expression of osteogenic markers and Wnt/β-catenin pathway-related genes, i...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Mohammed Al-Jarsha, Vladimíra Moulisová, Aldo Leal-Egaña, Andrew Connell, Kurt B Naudi, Ashraf F Ayoub, Matthew J Dalby, Manuel Salmerón-Sánchez
The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level...
May 14, 2018: ACS Biomaterials Science & Engineering
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