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https://www.readbyqxmd.com/read/28340520/galangin-inhibits-human-osteosarcoma-cells-growth-by-inducing-transforming-growth-factor-%C3%AE-1-dependent-osteogenic-differentiation
#1
Chunhong Liu, Mingming Ma, Junde Zhang, Shaoliu Gui, Xiaohai Zhang, Shuangtao Xue
Osteosarcoma is the most common primary malignancy of the musculoskeletal system, and is associated with excessive proliferation and poor differentiation of osteoblasts. Currently, despite the use of traditional chemotherapy and radiotherapy, no satisfactory and effective agent has been developed to treat the disease. Herein, we found that a flavonoid natural product, galangin, could significantly attenuate human osteosarcoma cells proliferation, without causing obvious cell apoptosis. Moreover, galangin enhanced the expression of osteoblast differentiation markers (collagen type I, alkaline phosphatase, osteocalcin and osteopontin) remarkably and elevated the alkaline phosphatase activity in human osteosarcoma cells...
March 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28340021/phenamil-an-amiloride-derivative-restricts-long-bone-growth-and-alters-keeled-sternum-bone-architecture-in-growing-chickens
#2
Tara R Price, Kristin Moncada, Hector Leyva-Jimenez, Kye Won Park, Peter Tontonoz, Rosemary L Walzem
"Broiler-type" chickens are fast-grow-ing, heavy-bodied birds with high demands on bone quality. Phenamil increased mineralization in cultured murine mesenchymal stem cells. Phenamil effects were tested in 2 groups of weight and gender matched day-old broiler chickens (n = 13). Oral administration of 30 mg phenamil/kg body weight d 1 to 13 reduced growth of chicks d 5 to 14 (P = 0.002); with phenamil-treated (PT) chick body weight being 84% of vehicle-treated (VT) chicks' body weight on d 14. Tissues collected on d 15 showed that femur lengths and widths did not differ, but tibias from PT chicks were 6% shorter (P = 0...
March 3, 2017: Poultry Science
https://www.readbyqxmd.com/read/28332248/citrate-attenuates-vascular-calcification-in-chronic-renal-failure-rats
#3
Yan Ou, Zengying Liu, Shuiqin Li, Xiaojing Zhu, Yan Lin, Jin Han, Zhaoyang Duan, Lining Jia, Baosong Gui
Vascular calcification (VC) is a major contributor of cardiovascular dysfunction in chronic renal failure (CRF). Citrate binds calcium and inhibits the growth of calcium crystals. This present study intends to evaluate the effect of citrate on VC in adenine-induced CRF rats. The rats were randomly divided into five groups: the control group, the citrate control group, model group, model rats with low-dose treatment of citrate (216 mg/kg) and model rats with high-dose treatment of citrate (746 mg/kg). The rats were euthanized at 5 weeks with their blood and aorta in detection...
March 23, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28332213/hen-egg-york-phosvitin-stimulates-osteoblast-differentiation-in-the-absence-of-ascorbic-acid
#4
Qingli Liu, Chunyan Li, Fang Geng, Xi Huang, Meihu Ma
BACKGROUND: Egg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on the osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3-E1 cells by using alkaline phosphatase (ALP) activity analysis, alizarin red S staining and real-time PCR assay. RESULTS: ALP activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3-E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28332167/controlled-release-of-vanadium-from-a-composite-scaffold-stimulates-mesenchymal-stem-cell-osteochondrogenesis
#5
S D Schussler, K Uske, P Marwah, F W Kemp, J D Bogden, S S Lin, Treena Livingston Arinzeh
Large bone defects often require the use of autograft, allograft, or synthetic bone graft augmentation; however, these treatments can result in delayed osseous integration. A tissue engineering strategy would be the use of a scaffold that could promote the normal fracture healing process of endochondral ossification, where an intermediate cartilage phase is later transformed to bone. This study investigated vanadyl acetylacetonate (VAC), an insulin mimetic, combined with a fibrous composite scaffold, consisting of polycaprolactone with nanoparticles of hydroxyapatite and beta-tricalcium phosphate, as a potential bone tissue engineering scaffold...
March 22, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28331312/investigation-of-silk-fibroin-nanoparticle-decorated-poly-l-lactic-acid-composite-scaffolds-for-osteoblast-growth-and-differentiation
#6
Biao-Qi Chen, Ranjith Kumar Kankala, Ai-Zheng Chen, Ding-Zhu Yang, Xiao-Xia Cheng, Ni-Na Jiang, Kai Zhu, Shi-Bin Wang
Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(l-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28326703/-effects-of-salvianolic-acid-b-on-osteogenic-differentiation-of-human-periodontal-ligament-cells
#7
Ma Yue, Ren Aishu, Fu Gang
OBJECTIVE: This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs). METHODS: Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs...
October 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28326342/short-term-intermittent-administration-of-parathyroid-hormone-facilitates-osteogenesis-by-different-mechanisms-in-cancellous-and-cortical-bone
#8
Kenji Ogura, Tadahiro Iimura, Yuji Makino, Ayano Sugie-Oya, Aya Takakura, Ryoko Takao-Kawabata, Toshinori Ishizuya, Keiji Moriyama, Akira Yamaguchi
Intermittent administration of human parathyroid hormone (1-34)[hPTH(1-34)] induces anabolic action on the bones. To understand the mechanism underlying the early phase of hPTH(1-34)-induced anabolic action, we investigated the expression profiles of osterix and sclerostin after short-term intermittent administration of hPTH(1-34) using immunohistochemistry in adult rats. In the cancellous bone, hPTH(1-34) administration greatly increased the number of osterix-positive cells in the bone marrow on day 1, but the cells gradually decreased on days 3 and 5...
December 2016: Bone Reports
https://www.readbyqxmd.com/read/28326297/coronary-artery-calcium-score-and-bone-metabolism-a-pilot-study-in-postmenopausal-women
#9
Pooneh Salari, Abbasali Keshtkar, Shapour Shirani, Leila Mounesan
BACKGROUND: Since 1991 many studies evaluated the link between cardiovascular diseases and osteoporosis, two age-related conditions, but the main common pathologic pathway has not been determined yet. The histological similarity between arterial calcified plaque and bone matrix and involvement of similar cells and mediators provide a special field of research. Therefore in the present study, we aimed to evaluate the relationship between coronary artery calcium score (CACS) as a surrogate marker of atherosclerosis and bone mediators and parameters in postmenopausal women...
February 2017: Journal of Bone Metabolism
https://www.readbyqxmd.com/read/28325291/systemic-rala-inos-nanoparticles-a-potent-gene-therapy-for-metastatic-breast-cancer-coupled-as-a-biomarker-of-treatment
#10
Cian M McCrudden, John W McBride, Joanne McCaffrey, Ahlam A Ali, Nicholas J Dunne, Vicky L Kett, Jonathan A Coulter, Tracy Robson, Helen O McCarthy
This study aimed to determine the therapeutic benefit of a nanoparticular formulation for the delivery of inducible nitric oxide synthase (iNOS) gene therapy in a model of breast cancer metastasis. Nanoparticles comprising a cationic peptide vector, RALA, and plasmid DNA were formulated and characterized using a range of physiochemical analyses. Nanoparticles complexed using iNOS plasmids and RALA approximated 60 nm in diameter with a charge of 25 mV. A vector neutralization assay, performed to determine the immunogenicity of nanoparticles in immunocompetent C57BL/6 mice, revealed that no vector neutralization was evident...
March 17, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28324131/case-report-fast-reversal-of-severe-osteoporosis-after-correction-of-excessive-levothyroxine-treatment-and-long-term-follow-up
#11
C M Laine, K Landin-Wilhelmsen
This case report describes a 38-year-old woman, who presented with bilateral femoral stress fractures and osteoporosis after years of excessive levothyroxine treatment. Her bone health was restored rapidly and long-lasting with the reduction of levothyroxine dosage. No bone-active treatment was warranted. INTRODUCTION: Hyperthyroidism is a known risk factor for osteoporosis and fractures. Recent studies on patients with serum thyrotropin-suppressive therapy have not, however, indicated adverse effects on bone during long-term follow-up...
March 21, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28324004/bone-turnover-is-suppressed-in-insulin-resistance-independent-of-adiposity
#12
K T Tonks, C P White, J R Center, D Samocha-Bonet, J R Greenfield
Context: The contribution of insulin resistance vs. adiposity to bone mineral density (BMD), bone turnover and fractures in humans remains unclear. Objective: Evaluate BMD and bone turnover markers (BTM) in lean (n=18) and overweight/obese individuals with (n=17) and without type 2 diabetes (n=34, deemed insulin-sensitive [Obsensitive, n=15] or insulin-resistant [Obresistant, n=19] by HOMA-IR). Design: Observational study. Outcome measures: Insulin sensitivity assessed by hyperinsulinemic-euglycemic clamp; whole body BMD and fat mass (FM) by DXA; and BTM (osteocalcin [OC], procollagen type-1 propeptide [P1NP] and collagen type-1 cross-linked C-telopeptide [CTx]) fasting and during clamp hyperinsulinemia...
January 23, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323991/whole-body-vibration-mimics-the-metabolic-effects-of-exercise-in-male-leptin-receptor-deficient-mice
#13
Meghan E McGee-Lawrence, Karl H Wenger, Sudipta Misra, Catherine L Davis, Norman K Pollock, Mohammed Elsalanty, Kehong Ding, Carlos M Isales, Mark W Hamrick, Joanna R Erion, Marlena Wosiski-Kuhn, Phonepasong Arounleut, Mark P Mattson, Roy G Cutler, Jack C Yu, Alexis M Stranahan
Whole-body vibration has gained attention as a potential exercise mimetic, but direct comparisons with the metabolic effects of exercise are scarce. To determine whether whole-body vibration recapitulates the metabolic and osteogenic effects of physical activity, we exposed male wildtype (Wt) and leptin receptor deficient (db/db) mice to daily treadmill exercise or whole-body vibration for three months. Body weights were analyzed and compared with Wt and db/db mice that remained sedentary. Glucose and insulin tolerance testing revealed comparable attenuation of hyperglycemia and insulin resistance in db/db mice following treadmill exercise or whole-body vibration...
March 15, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323966/persistent-reduction-of-circulating-myeloid-calcifying-cells-in-acromegaly-relevance-to-the-bone-vascular-axis
#14
Gian Paolo Fadini, Francesca Dassie, Roberta Cappellari, Mariasara Persano, Saula Vigili de Kreutzenberg, Chiara Martini, Matteo Parolin, Angelo Avogaro, Roberto Vettor, Pietro Maffei
Context: Acromegaly is a systemic disease characterized by persistent bone pathology and excess cardiovascular mortality. Despite multiple concomitant risk factors, atherosclerosis does not seem to be accelerated in acromegaly. Objective: To compare the levels of circulating myeloid calcifying cells (MCCs), which promote ectopic calcification and inhibit angiogenesis, in individuals with or without acromegaly. Design: Cross-sectional case-control study...
March 17, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323146/hypogonadal-men-with-type-2-diabetes-mellitus-have-smaller-bone-size-and-lower-bone-turnover
#15
Georgia Colleluori, Lina Aguirre, Richard Dorin, David Robbins, Dean Blevins, Yoann Barnouin, Rui Chen, Clifford Qualls, Dennis T Villareal, Reina Armamento-Villareal
INTRODUCTION: Both hypogonadism and type 2 diabetes mellitus (T2D) are associated with increased fracture risk. Emerging data support the negative effect of low testosterone on glucose metabolism, however, there is little information on the bone health of hypogonadal men with diabetes. We evaluated the bone mineral density (BMD), bone geometry and bone turnover of hypogonadal men with T2D compared to hypogonadal men without diabetes. MATERIALS AND METHODS: Cross-sectional study, men 40-74years old, with average morning testosterone (done twice) of<300ng/dl...
March 16, 2017: Bone
https://www.readbyqxmd.com/read/28321876/effects-of-glucose-concentration-on-osteogenic-differentiation-of-type-ii-diabetes-mellitus-rat-bone-marrow-derived-mesenchymal-stromal-cells-on-a-nano-scale-modified-titanium
#16
I Yamawaki, Y Taguchi, S Komasa, A Tanaka, M Umeda
BACKGROUND AND OBJECTIVE: Diabetes mellitus (DM) is a common disease worldwide. Patients with DM have an increased risk of losing their teeth compared with other individuals. Dental implants are a standard of care for treating partial or full edentulism, and various implant surface treatments have recently been developed to increase dental implant stability. However, some studies have reported that DM reduces osseointegration and the success rate of dental implants. The purpose of this study was to determine the effects of high glucose levels for hard tissue formation on a nano-scale modified titanium surface...
March 21, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28303237/the-effect-of-biomechanical-stimulation-on-osteoblast-differentiation-of-human-jaw-periosteum-derived-stem-cells
#17
Ju-Min Lee, Min-Gu Kim, June-Ho Byun, Gyoo-Cheon Kim, Jung-Hoon Ro, Dae-Seok Hwang, Byul-Bora Choi, Geun-Chul Park, Uk-Kyu Kim
BACKGROUND: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor. METHODS: Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium...
December 2017: Maxillofacial Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28301384/the-effects-of-hierarchical-micro-nano-structured-titanium-surface-on-osteoblast-proliferation-and-differentiation-under-diabetic-conditions
#18
Huanhuan Jiang, Xiang Ma, Wenjuan Zhou, Kai Dong, Xiaohui Rausch-Fan, Shutai Liu, Shu Li
PURPOSE: The aim of the present study was to mimic the hierarchical structure of bone tissues by simple sandblasting/acid-etching and anodization to investigate the effects of such surface characteristics on proliferation and differentiation of osteoblasts in high glucose concentrations. By the way, the effects of high glucose levels on osteoblast functions were tested. METHODS: MC3T3-E1 cells cultured on sand-blasted and acid-etched (SLA) surface and nano-modified SLA (NMSLA) surface were subjected to normal serum (NS) and diabetic serum (DS), respectively...
April 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28299448/capacity-of-muscle-derived-stem-cells-and-pericytes-to-promote-tendon-graft-integration-and-ligamentization-following-anterior-cruciate-ligament-reconstruction
#19
Tomislav Ćuti, Maja Antunović, Inga Marijanović, Alan Ivković, Andreja Vukasović, Igor Matić, Marko Pećina, Damir Hudetz
PURPOSE: The aim of this study is to examine the capacity of muscle tissue preserved on hamstring tendons forming candy-stripe grafts in order to improve tendon to bone ingrowth and ligamentization. We hypothesized that muscle tissue does possess a stem cell population that could enhance the healing process of the ACL graft when preserved on the tendons. METHODS: Human samples from gracilis and semitendinosus muscles were collected during ACL surgery from ten patients and from these tissue samples human muscle-derived stem cells and tendon-derived stem cells were isolated and propagated...
March 15, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28298072/the-anti-osteoporotic-and-anti-atherogenic-effects-of-alendronate-and-simvastatin-in-ovariectomized-rats-fed-high-fat-diet-a-comparative-study-of-combination-therapy-versus-monotherapy
#20
Maha Tarek Mohamed, Sally A Abuelezz, Suzi Sobhy Atalla, Lobna Fouad Abd El Aziz, Sonia Salib Gorge
AIM: Epidemiological studies suggest a possible link between osteoporosis and cardiovascular diseases. Mevalonate pathway was pointed to as a part of this link. This study was done to investigate the effects of Alendronate (Al) and Simvastatin (Sim), both act on the mevalonate pathway, on osteoporosis, dyslipidemia and atherosclerotic changes in ovariectomized (OVX) rats fed high fat diet (HFD). MAIN METHODS: 60 female albino rats were equally divided into 5 groups: control sham, OVX-HFD untreated, OVX -HFD treated with Al (3mg/kg/d) or/and Sim (6mg/kg/d)...
March 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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