keyword
MENU ▼
Read by QxMD icon Read
search

haemonchus contortus

keyword
https://www.readbyqxmd.com/read/28316426/genotyping-of-benzimidazole-resistant-and-susceptible-isolates-of-haemonchus-contortus-from-sheep-by-allele-specific-pcr
#1
Karthik Mohanraj, Subhra Subhadra, Aravindan Kalyanasundaram, Manikkavasagan Ilangopathy, Muthusamy Raman
Extensive and indiscriminate use of the benzimidazole class of drugs has led to the onset of anthelmintic resistance. In tropical countries like India, Haemonchus contortus is the most pathogenic parasite infecting sheep and goats. The widespread presence of resistant helminths (especially H. contortus) threatens the livestock farming. The use of various drugs has led to single nucleotide polymorphism that causes specific amino acid substitutions in β-tubulin protein of H. contortus to confer resistance. This emphasizes the need for a survey on the present status of resistance in India...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28288755/anthelmintic-activity-of-plant-extracts-from-brazilian-savanna
#2
Andreia F Oliveira, Livio M Costa Junior, Aldilene S Lima, Carolina R Silva, Maria N S Ribeiro, José W C Mesquista, Cláudia Q Rocha, Marcelo M P Tangerina, Wagner Vilegas
Helminth infections represent a serious problem for the production of small ruminants that is currently aggravated by resistance to anthelmintic products and has induced a search for control alternatives, such as natural products. In this study, extracts of Turnera ulmifolia L. (leaves and roots), Parkia platycephala Benth. (leaves and seeds) and Dimorphandra gardneriana Tul. (leaves and bark), which have been cited in ethnoveterinary studies and selected naturally by goats in the cerrado (Brazilian savanna), were tested in vitro against Haemonchus contortus...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28262394/the-in-vitro-anthelmintic-properties-of-browse-plant-species-against-haemonchus-contortus-is-determined-by-the-polyphenol-content-and-composition
#3
G Mengistu, H Hoste, M Karonen, J-P Salminen, W H Hendriks, W F Pellikaan
The aims of the present study were to (a) evaluate the anthelmintic activity of 10 East African browse plant extracts, (b) examine their role in inhibition of Haemonchus contortus larval exsheathment, (c) establish relationship between inhibition of larval exsheathment and browse plant extract polyphenol composition. Acetone/water (70/30%) extracts of air dried leaves of Acacia etbaica, Cadaba farinosa, Capparis tomentosa, Dichrostachys cinerea, Dodonaea angustifolia, Euclea racemosa, Maerua angolensis, Maytenus senegalensis, Rhus natalensis and Senna singueana were used...
December 31, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/28259774/exploration-of-interaction-zones-of-%C3%AE-tubulin-colchicine-binding-domain-of-helminths-and-binding-mechanism-of-anthelmintics
#4
Prabodh Ranjan, Sivakumar Prasanth Kumar, Vijayakrishna Kari, Prakash Chandra Jha
Numerous studies postulated the possible modes of anthelmintic activity by targeting alternate or extended regions of colchicine binding domain of helminth β-tubulin. We present three interaction zones (zones vide -1 to -3) in the colchicine binding domain of Haemonchus contortus (a helminth) β-tubulin homology model and developed zone-wise structure-based pharmacophore models coupled with molecular docking technique to unveil the binding hypotheses. The resulted ten structure-based hypotheses were then refined to essential three point pharmacophore features that captured recurring and crucial non-covalent receptor contacts and proposed three characteristics necessary for optimal zone-2 binding: a conserved pair of H bond acceptor (HBA to form H bond with Asn226 residue) and an aliphatic moiety of molecule separated by 3...
February 24, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28250429/exploring-the-microbial-community-microflora-associated-with-ovine-haemonchus-contortus-macroflora-field-strains
#5
Saeed El-Ashram, Xun Suo
High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the microbial community associated with the different stages of the life-cycle of ovine Haemonchus contortus field strains using two distinct amplification primer sets (targeting V3-V4, and V5-V7). Scanning electron microscope and polymerase chain reaction coupled with Illumina MiSeq platform were employed to confirm the absence of any parasite surface contamination by intact bacteria or their DNA products...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28246451/gastrointestinal-parasitism-of-goats-in-hilly-region-of-meghalaya-india
#6
Meena Das, R Laha, A Goswami, A Goswami
AIM: The aim of this study was to determine the prevalence of gastrointestinal (GI) parasitic infections in goats of hilly region of Meghalaya. MATERIALS AND METHODS: A total of 834 fecal samples of goats were screened for 1 year (2014-2015) using flotation techniques. RESULTS: The overall prevalence of GI parasitic infections in goats was 28.65%. Season-wise highest infections were recorded during rainy season (34.92%) followed by cool (26...
January 2017: Veterinary World
https://www.readbyqxmd.com/read/28242041/a-novel-snapback-primer-probe-assay-for-the-detection-and-discrimination-of-sympatric-haemonchus-species-using-dna-melting-analysis
#7
Rudolf Pichler, Katja Silbermayr, Kathiravan Periasamy
Different sympatric species of Haemonchus parasites infecting ruminants and camels can be distinguished morphologically, but involves tedious microscopic examinations, measurements and several other limitations. Information on internal transcribed spacer-2 (ITS-2) sequence provides confirmatory differentiation of sympatric Haemonchus species. The present study introduces a novel, snapback primer probe based, real time PCR assay for the differentiation of three sympatric Haemonchus species, H. contortus (Hco), H...
February 20, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28239871/the-effect-of-ovine-peripheral-blood-mononuclear-cells-on-haemonchus-contortus-larval-morbidity-in-vitro
#8
Elizabeth A Shepherd, Javier J Garza, Scott P Greiner, Scott A Bowdridge
The objective of this study was to determine the effects of peripheral blood mononuclear cells (PBMC), derived from parasite-resistant St. Croix (STC) hair sheep and parasite-susceptible Suffolk (SUF) sheep, on Haemonchus contortus L3 stage larval death in vitro, with or without autologous serum. Larval morbidity was quantified by measuring larval ATP concentration following incubation with PBMC. Larvae exposed to either STC- or SUF-derived PBMC had lower ATP than live larvae (0.12 μM ATP and 0.16 μM ATP versus 0...
February 26, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28238472/modulation-of-haemonchus-contortus-infection-by-depletion-of-%C3%AE-%C3%AE-t-cells-in-parasite-resistant-canaria-hair-breed-sheep
#9
Julia N Hernández, Els Meeusen, Michael Stear, Francisco Rodríguez, David Piedrafita, Jorge F González
Canaria Hair Breed (CHB) sheep display resistance against the adult stage of the nematode, Haemonchus contortus. Previous studies have suggested significant correlations between γδ(+) T lymphocytes and fecundity of female adult worms, suggesting a novel role in immune modulation by these cells. The largest proportion of γδ(+) T lymphocytes in sheep are the subpopulation of γδ(+)/WC1(+) T cells. The aim of this study was to evaluate the effect of γδ(+)⁄WC1(+) T cell depletion via infusion of anti-γδ/WC1 monoclonal antibody (mAb) on the subsequent immune response of CHB sheep infected with H...
February 21, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28146155/predatory-activity-of-butlerius-nematodes-and-nematophagous-fungi-against-haemonchus-contortus-infective-larvae
#10
Manoel Eduardo da Silva, Miguel Angel Mercado Uriostegui, Jair Millán-Orozco, Pedro Mendoza de Gives, Enrique Liébano Hernández, Fabio Ribeiro Braga, Jackson Victor de Araújo
The purpose of this study was to evaluate the predatory activity of the nematode Butlerius spp. and fungal isolates of Duddingtonia flagrans, Clonostachys rosea, Arthrobotrys musiformis and Trichoderma esau against H. contortus infective larvae (L3) in grass pots. Forty-eight plastic gardening pots containing 140 g of sterile soil were used. Panicum spp. grass seeds (200 mg) were sown into each pot and individually watered with 10 mL of tap water. Twelve days after seeding, the pots were randomly divided into 6 groups (n=8)...
January 26, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28144765/control-of-haemonchus-contortus-in-sheep-using-basidiocarps-of-agaricus-blazei-murril
#11
Thallyta Maria Vieira, Leydiana Duarte Fonseca, Gabriela Almeida Bastos, Viviane de Oliveira Vasconcelos, Maria Luíza França Silva, Franciellen Morais-Costa, Adriano Vinícius de Paiva Ferreira, Neide Judith Faria de Oliveira, Eduardo Robson Duarte
OBJECTIVE: This study evaluated the effects in vitro and in vivo of Agaricus blazei against Haemonchus contortus in sheep. METHODS: The in vitro efficacy of aqueous extract on egg hatching inhibition (EHI) was investigated and after 72 h incubation with varying concentrations the effects on, blastomeres, embryonated eggs, and first stage larvae (L1) were evaluated. Larval development inhibition (LDI) for dry powder and the aqueous extract were evaluated in fecal cultures of sheep infected with H...
January 31, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28127366/antigenic-cross-reactivity-among-haemonchus-contortus-oesophagostomum-columbianum-and-trichuris-ovis-of-goat
#12
Ruma Jas, Joydeb Ghosh, Kinsuk DAS
BACKGROUND: Cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. Mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition. METHODS: The present study was undertaken to detect antigenic cross-reactivity among Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis of goats by SDS-PAGE and western blot analysis using hyperimmune sera (HIS) rose in rabbit separately against the antigens of the three nematode species...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28098507/development-and-evaluation-of-a-loop-mediated-isothermal-amplification-lamp-assay-for-the-detection-of-haemonchus-contortus-in-goat-fecal-samples
#13
Xin Yang, Mingwei Qi, Zongze Zhang, Chong Gao, Chunqun Wang, Weiqiang Lei, Li Tan, Junlong Zhao, Rui Fang, Min Hu
Haemonchus contortus is one of the most significant strongylid nematodes infecting small ruminants and causes great economic losses to the livestock industry worldwide. Accurate diagnosis of H. contortus is crucial to control the infection. Traditional microscopic examinations are the most common methods for the diagnosis of H. contortus, but they are time-consuming and inaccurate. Molecular methods based on PCR are more accurate, but need expensive machines usually only used in the laboratory. Loop-mediated isothermal amplification (LAMP) is a rapid, simple, specific and sensitive method that has been widely used to detect viruses, bacteria and parasites...
January 18, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28096603/gastrointestinal-nematode-larvae-in-the-grazing-land-of-cattle-in-guwahati-assam
#14
Meena Das, D K Deka, S Islam, P C Sarmah, K Bhattacharjee
AIM: To know the prevalence of gastrointestinal nematode larvae (L3) in the grazing land of cattle in Guwahati, Kamrup district, Assam. MATERIALS AND METHODS: Pastures were collected and examined for the presence of nematode larvae (L3) from six localities of Guwahati at monthly interval from August 2012 to July 2013. The counted larvae were then expressed as per kg dry matter of herbage (L3/kg DM). RESULTS: Examination of pastures revealed presence of nematode larvae (L3) in pastures throughout the year which varied from 4...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096462/unique-contributions-of-an-arginine-side-chain-to-ligand-recognition-in-a-glutamate-gated-chloride-channel
#15
Timothy Lynagh, Vitaly V Komnatnyy, Stephan A Pless
Glutamate recognition by neurotransmitter receptors often relies on Arg residues in the binding site, leading to the assumption that charge-charge interactions underlie ligand recognition. However, assessing the precise chemical contribution of Arg side chains to protein function and pharmacology has proven to be exceedingly difficult in such large and complex proteins. Using the in vivo nonsense suppression approach, we report the first successful incorporation of the isosteric, titratable Arg analog, canavanine, into a neurotransmitter receptor in a living cell, utilizing a glutamate-gated chloride channel from the nematode Haemonchus contortus Our data unveil a surprisingly small contribution of charge at a conserved arginine side chain previously suggested to form a salt bridge with the ligand, glutamate...
March 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28095895/genome-wide-snp-analysis-using-2b-rad-sequencing-identifies-the-candidate-genes-putatively-associated-with-resistance-to-ivermectin-in-haemonchus-contortus
#16
Xiaoping Luo, Xiaona Shi, Chunxiu Yuan, Min Ai, Cheng Ge, Min Hu, Xingang Feng, Xiaoye Yang
BACKGROUND: The excessive and uncontrolled use of anthelmintics, e.g. ivermectin (IVM) for the treatment of livestock parasites has led to widespread resistance in gastrointestinal nematodes, such as Haemonchus contortus. There is an urgent need for better management of drug-use in nematode control and development of novel anthelmintics. Discovery and identification of anthelmintic resistance-associate molecules/markers can provide a basis for rational anthelmintics-use and development of novel drugs...
January 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28091356/resistance-to-gastrointestinal-parasite-infection-in-djallonk%C3%A3-sheep
#17
A Traoré, D R Notter, A Soudre, A Kaboré, I Álvarez, I Fernández, M Sanou, M Shamshuddin, K Periasamy, H H Tamboura, F Goyache
Gastrointestinal parasitism places serious constraints on small ruminant production. The situation has been exacerbated by development of drug resistance in many parasite populations, leading to interest in identification of animals with genetically mediated resistance or tolerance to nematode infections. This study assessed the response to natural infection with gastrointestinal nematodes (GIN) in Djallonké sheep during the rainy season in the Sudan-Guinea Savannah region of Burkina Faso. Haemonchus contortus is the most prevalent GIN at this site and time...
January 16, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28069083/pcr-primers-for-straightforward-differentiation-of-haemonchus-contortus-haemonchus-placei-and-their-hybrids
#18
M R V Amarante, M C Santos, C C Bassetto, A F T Amarante
Haemonchus contortus and Haemonchus placei are among the major parasites of small ruminants and cattle. Although infection with these nematodes is host-specific, with H. placei predominating in cattle and H. contortus in sheep, cross-infections are observed in areas where both parasites are sympatric, and hybrid offspring can occur. Therefore, a fast and precise method is required for differentiating the parasites. Identification based on spicule morphometry is the most common technique for differentiating Haemonchus species...
January 10, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28063162/serum-mediated-haemonchus-contortus-larval-aggregation-differs-by-larval-stage-and-is-enhanced-by-complement
#19
Javier J Garza, Scott P Greiner, Scott A Bowdridge
The objectives of this study were to measure Haemonchus contortus larval aggregation by complement/antibody complexes, determine effect of breed resistance and infection status, and determine the effect of larval maturation on larval aggregation in vitro. Larval binding assays were performed on H. contortus L3, exsheathed L3 and L4 incubated with serum from either parasite naïve or H. contortus primed St. Croix (resistant) and Suffolk (susceptible) lambs. No differences in L3 aggregation were observed between serum from either breed or infection status...
January 7, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28032300/genotypic-and-phenotypic-evaluation-for-benzimidazole-resistance-or-susceptibility-in-haemonchus-contortus-isolates
#20
Waleed M Arafa, Patricia J Holman, Thomas M Craig
Haemonchus contortus isolates were evaluated for benzimidazole (BZ) resistance or susceptibility by allele-specific PCR based on β-tubulin isotype 1 gene polymorphisms at the F167Y, E198A, and F200Y sites. Two isolates, one presumed susceptible from wild pronghorn antelope (PH) and one known to be resistant from goats (VM), were also assayed phenotypically for BZ resistance or susceptibility in the larval development assay (Drenchrite®). The BZ EC50 was 0.198 μM (intermediate between susceptible and weak resistant) for PH with critical well 5 (intermediate between susceptible and weak resistant) and 1...
February 2017: Parasitology Research
keyword
keyword
35211
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"