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haemonchus contortus

Henry Y Gu, Neil D Marks, Alan D Winter, William Weir, Thomas Tzelos, Tom N McNeilly, Collette Britton, Eileen Devaney
microRNAs are small non-coding RNAs that are important regulators of gene expression in a range of animals, including nematodes. We have analysed a cluster of four miRNAs from the pathogenic nematode species Haemonchus contortus that are closely linked in the genome. We find that the cluster is conserved only in clade V parasitic nematodes and in some ascarids, but not in other clade III species nor in clade V free-living nematodes. Members of the cluster are present in parasite excretory-secretory products and can be detected in the abomasum and draining lymph nodes of infected sheep, indicating their release in vitro and in vivo...
November 16, 2017: PLoS Neglected Tropical Diseases
Jean-Paul Déto Ursul N'Guessan, Pierre-Olivier Delaye, Mélanie Pénichon, Claude L Charvet, Cédric Neveu, Mahama Ouattara, Cécile Enguehard-Gueiffier, Alain Gueiffier, Hassan Allouchi
We report the synthesis of a series of imidazo[1,2-a]pyridine-based molecules as anthelmintic against the livestock parasite Haemonchus contortus. The molecules were tested by using Larval Paralysis Test (LPT), in order to target ionic channels, as most of the prominent marketed anthelminthics present such mechanism of action. The most active compound (5e) displayed paralysis on H. contortus stage 3 larvae until 31.25 µM. Effect of 5e on H. contortus cholinergic receptors (L-AChR1 and 2) was characterized via electrophysiological measurement and a rare antagonist mode of action was unveiled...
November 6, 2017: Bioorganic & Medicinal Chemistry
S Cériac, C Jayles, R Arquet, D Feuillet, Y Félicité, H Archimède, J-C Bambou
BACKGROUND: Gastrointestinal nematode (GIN) remains the most important pathogenic constraint of small ruminant production worldwide. The improvement of the host immune response against GIN though breeding for improved animal resistance, vaccination and nutritional supplementation appear as very promising methods. The objective of this study was to investigate the effect of four nutritional status differing in protein and energy levels (Hay: 5.1 MJ/Kg of dry matter (DM) and 7.6% of crude protein (CP), Ban: 8...
November 9, 2017: BMC Veterinary Research
Lucas Atehmengo Ngongeh
Faecal worm egg counts (FEC) are very important indicators in assessing the intensity of helminth infections in animal hosts and inform decisions taken in parasite control programmes. FEC are however affected by many factors which include the dose level of larval challenge, species composition of the worms, worm burden, female worm fecundity and concurrent infections to name but these few. The relevance of time of collection of faecal sample for FEC is not fully known and in most cases faeces for analysis is collected whenever feasible for the researcher on sample collection days...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Omnia M Kandil, Irene S Gamil, Seham H M Hendawy, Fatma Medhat, Ola H El-Habit
Haemonchus contortus is one of the most pathogenic and economically important parasites of sheep. Different H. contortus antigens; crude somatic antigen (CSA), excretory/secretory antigen (ESA), crude larval antigen (CLA), glutathione-S-transferase antigen (GST) and recombinant protein (rhcp 26/23) were prepared and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot. The antigens were immunologically evaluated through indirect enzyme linked immunosorbent assay (ELISA) for diagnosis of haemonchosis in experimentally and naturally infected sheep...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Neil D Sargison, Elizabeth Redman, Alison A Morrison, David J Bartley, Frank Jackson, Hardeep Naghra-van Gijzel, Nancy Holroyd, Matthew Berriman, James A Cotton, John S Gilleard
Parasitic nematode species have extremely high levels of genetic diversity, presenting a number of experimental challenges for genomic and genetic work. Consequently, there is a need to develop inbred laboratory strains with reduced levels of polymorphism. The most efficient approach to inbred line development is single pair mating, but this is challenging for obligate parasites where the adult sexual reproductive stages are inside the host, and thus difficult to experimentally manipulate. This paper describes a successful approach to single pair mating of a parasitic nematode, Haemonchus contortus...
October 27, 2017: International Journal for Parasitology
Saeed El-Ashram, Ibrahim Al Nasr, Fathi Abouhajer, Maged El-Kemary, Guangping Huang, Güngör Dinçel, Rashid Mehmood, Min Hu, Xun Suo
The interactions between gastric microbiota, ovine host, and Haemonchus contortus portray the ovine gastric environment as a complex ecosystem, where all factors play a pertinent role in fine-tuning each other and in haemeostasis. We delineated the impact of early and late Haemonchus infection on abomasal and ruminal microbial community, as well as the ovine host. Twelve, parasite-naive lambs were divided into four groups, 7 days post-infection (dpi) and time-matched uninfected-control groups; 50 dpi and time-matched uninfected control groups were used for the experiment...
December 2017: Veterinary Research Communications
Anna M Pyziel, Sven Björck, Rikard Wiklund, Moa Skarin, Aleksander W Demiaszkiewicz, Johan Höglund
The history of European bison Bison bonasus Linnaeus, 1758 has been stormy since its extinction in the wild after the First World War. Due to the fact that the species was restored from just 12 founders, further expansion has suffered from low genetic variability, rendering the bison vulnerable to various pathogens due to inbreeding depression. Parasites are recognised as a key biological threat to bison population. Thus, parasitological examination including monitoring of the level of anthelmintic resistance in a herd should be a routine procedure involved in management and protection of European bison...
October 31, 2017: Parasitology Research
J Ventura-Cordero, P G González-Pech, P R Jaimez-Rodriguez, G I Ortiz-Ocampo, C A Sandoval-Castro, J F J Torres-Acosta
Previous cafeteria studies suggested that a moderate natural gastrointestinal nematode (GIN) infection did not modify the resource selection of adult Criollo goats towards tannin-rich plants compared with worm-free goats. A higher infection with Haemonchus contortus could trigger a change in the resource selection behaviour towards tannin-rich foliage. Alternatively, goats might select plant species solely to meet their nutritional requirements. A cafeteria study investigated the effect of a high artificial infection with H...
October 26, 2017: Animal: An International Journal of Animal Bioscience
Yi Yang, Xiaolu Guo, Hongli Zhang, Yan Huang, Xueqiu Chen, Aifang Du
BACKGROUND: Haemonchus contortus is a serious parasitic nematode in domestic ruminants around the world, including China. Haemonchus contortus has developed extensive resistance to commercial anthelmintics, which has produced a demand for new control methods, such as more effective drugs. Gerbils infected with H. contortus have previously been used as a model for anthelmintics selections, and the growth of H. contortus had been briefly examined. To enhance the model, this study provides an additional description of the development of H...
October 23, 2017: Parasites & Vectors
G S Castañeda-Ramírez, J F J Torres-Acosta, C A Sandoval-Castro, P G González-Pech, V P Parra-Tabla, C Mathieu
In vitro studies using plant extracts suggest a relationship between their polyphenol contents and their anthelmintic (AH) activity against Haemonchus contortus. High polyphenol content appears to increase the efficacy of plant extracts against H. contortus as assessed by the larval exsheathment inhibition assay (LEIA) while appearing to reduce the AH efficacy measured using the egg hatch assay (EHA). In addition, some plants lack AH activity. Therefore, the present study investigated the relationship between the contents of condensed tannins (CT), total phenols (TP), and total tannins (TT) in methanol:water extracts (70:30) obtained from ten tropical plant species consumed by small ruminants as well as their AH activity against H...
October 18, 2017: Parasitology Research
Esra Yilmaz, Sabrina Ramünke, Janina Demeler, Jürgen Krücken
Benzimidazoles (BZs) remain amongst the most widely used anthelmintic drug classes against gastro-intestinal nematode infections, although their efficacy is increasingly compromised by resistance. The primary underlying mechanisms for BZ resistance are single-nucleotide polymorphisms (SNPs) in the isotype 1 β-tubulin gene causing the substitutions F167Y, E198A or F200Y. However, resistance is believed to be multi-genic and previous studies have shown that isolates carrying 90-100% F200Y can vary considerably in their resistance level in the egg hatch assay (EHA)...
October 7, 2017: International Journal for Parasitology, Drugs and Drug Resistance
Iveta A Kyriánová, Jaroslav Vadlejch, Oldřich Kopecký, Iva Langrová
This study evaluated patterns and species composition of parasitic infections detected over a 1-year period at an organic goat farm. As a result of coprological examination, the overall prevalence of observed strongylids (99%), coccidia of the genus Eimeria (98%), and Muellerius capillaris lungworms (93%) was calculated. The most prevalent strongylids recovered from incubated fecal samples were Haemonchus contortus (42%), genera Trichostrongylus (23%), Oesophagostomum columbianum (13%), and Teladorsagia circumcincta (11%)...
November 2017: Parasitology Research
Weibson Paz Pinheiro André, Géssica Soares Cavalcante, Wesley Lyeverton Correia Ribeiro, Jessica Maria Leite Dos Santos, Iara Tersia Freitas Macedo, Haroldo César Beserra de Paula, Selene Maia de Morais, Janaina Viana de Melo, Claudia Maria Leal Bevilaqua
Thymol is a monoterpene and acetylation form of this compound can reduce the toxicity and enhance its biological effects. The objective of this study was to evaluate the effect of thymol and thymol acetate (TA) on egg, larva and adult Haemonchus contortus and the cuticular changes, acute toxicity in mice and the efficacy on sheep gastrointestinal nematodes. In vitro tests results were analyzed by analysis of variance (ANOVA) and followed by comparison with Tukey test or Bonferroni. The efficacy of in vivo test was calculated by the BootStreet program...
July 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
L M Katiki, A C P Gomes, A M E Barbieri, P A Pacheco, L Rodrigues, C J Veríssimo, G Gutmanis, A M Piza, H Louvandini, J F S Ferreira
Haemonchus contortus is the most important nematode in small ruminant systems, and has developed tolerance to all commercial anthelmintics in several countries. In vitro (egg hatch assay) and in vivo tests were performed with a multidrug strain of Haemonchus contortus using Terminalia catappa leaf, fruit pulp, and seed extracts (in vitro), or pulp and seed powder in lambs experimentally infected with H. contortus. Crude extracts from leaves, fruit pulp and seeds obtained with 70% acetone were lyophilized until used...
November 15, 2017: Veterinary Parasitology
Ana Cláudia Alexandre de Albuquerque, Cesar Cristiano Bassetto, Fabiana Alves de Almeida, Alessandro F T Amarante
This study examined the development of resistance to anthelmintics in Haemonchus contortus in lambs under suppressive or selective treatment regimens that included monepantel. Twenty Ile de France and 20 Santa Ines lambs were allocated to two anthelmintic treatment regimens, based on body weight and nematode faecal egg counts (FEC): targeted selective treatment (TST) or suppressive treatment, both with monepantel. Lambs of the TST group were treated individually when they presented with a packed cell volume (PCV) ≤20%...
November 15, 2017: Veterinary Parasitology
Saeed El-Ashram, Xun Suo
A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.
September 29, 2017: Scientific Reports
Bo-Bo Wang, Kui-Zheng Cai, Qiang Xu, Jun-Lin Liu, Yu-Jia Xue, Kang-Ying Wang, Rui Ren, Wen-Xiu Fang, Fan Wang, Xin Cao
It is necessary to screen more potential candidates from the local isolates of nematophagous fungi and to investigate interaction between the fungal strains and the nematodes for the biological control of parasitic nematodes in livestocks. In the present study, The present study was divided into two phases. In the first phase, it was to bewe assessed that the in vitro predatory activity and the viability of isolates of Arthrobotrys thaumasia (Monacrosporium thaumasium) after passing through the gastrointestinal tract of sheep...
September 27, 2017: Journal of Parasitology
Beatriz Munguía, Ramiro Teixeira, Victoria Veroli, Elisa Melian, Jenny Saldaña, Mahia Minteguiaga, Mario Señorale, Mónica Marín, Laura Domínguez
Microtubules are non-covalent cylindrical polymers formed by alpha- and beta-tubulin heterodimer units, crucial for cell division, intracellular transport, motility and differentiation. This makes them very attractive pharmacological targets exploited to develop different drugs such as anthelmintics, antifungals, and antineoplastics. In this work, in order to establish an in vitro target-based screen to integrate to the search for new anthelmintics, we explored the extraction of native assembly-competent tubulin from two helminth parasites: Mesocestoides vogae tetrathyridia (syn...
November 2017: Experimental Parasitology
Dong-Dong Shen, Ji-Fei Wang, Dan-Yu Zhang, Zhi-Wei Peng, Tian-Yun Yang, Zhao-Ding Wang, Dwight D Bowman, Zhi-Jun Hou, Zhen-Sheng Liu
BACKGROUND: Haemonchus contortus is known among parasitic nematodes as one of the major veterinary pathogens of small ruminants and results in great economic losses worldwide. Human activities, such as the sympatric grazing of wild with domestic animals, may place susceptible wildlife hosts at risk of increased prevalence and infection intensity with this common small ruminant parasite. Studies on phylogenetic factors of H. contortus should assist in defining the amount of the impact of anthropogenic factors on the extent of sharing of agents such as this nematode between domestic animals and wildlife...
September 19, 2017: Parasites & Vectors
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