keyword
https://read.qxmd.com/read/38316581/tumor-microenvironment-ros-ph-cascade-responsive-supramolecular-nanoplatform-with-ros-regeneration-property-for-enhanced-hepatocellular-carcinoma-therapy
#1
JOURNAL ARTICLE
Jinfeng Shi, Yehui Wang, Yihan Wu, Jingjing Li, Chaomei Fu, Yi Li, Xingliang Xie, Xiaohong Fan, Yichen Hu, Chuan Hu, Jinming Zhang
The low targeted drug delivery efficiency, including poor tumor accumulation and penetration and uncontrolled drug release, leads to the failure of cancer therapy. Herein, a multifunctional supramolecular nanoplatform loading triptolide (TPL/PBAETK @GA NPs) was fabricated via the host-guest interaction between glycyrrhetinic-acid-modified poly(ethylene glycol)-adamantanecarboxylic acid moiety and reactive oxygen species (ROS)/pH cascade-responsive copolymer poly(β-amino esters)-thioketal (TK)-β-cyclodextrin...
February 5, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38287189/optineurin-provides-a-mitophagy-contact-site-for-tbk1-activation
#2
JOURNAL ARTICLE
Koji Yamano, Momoha Sawada, Reika Kikuchi, Kafu Nagataki, Waka Kojima, Ryu Endo, Hiroki Kinefuchi, Atsushi Sugihara, Tomoshige Fujino, Aiko Watanabe, Keiji Tanaka, Gosuke Hayashi, Hiroshi Murakami, Noriyuki Matsuda
Tank-binding kinase 1 (TBK1) is a Ser/Thr kinase that is involved in many intracellular processes, such as innate immunity, cell cycle, and apoptosis. TBK1 is also important for phosphorylating the autophagy adaptors that mediate the selective autophagic removal of damaged mitochondria. However, the mechanism by which PINK1-Parkin-mediated mitophagy activates TBK1 remains largely unknown. Here, we show that the autophagy adaptor optineurin (OPTN) provides a unique platform for TBK1 activation. Both the OPTN-ubiquitin and the OPTN-pre-autophagosomal structure (PAS) interaction axes facilitate assembly of the OPTN-TBK1 complex at a contact sites between damaged mitochondria and the autophagosome formation sites...
January 29, 2024: EMBO Journal
https://read.qxmd.com/read/38274644/decreased-femur-tunnel-widening-after-augmented-suspensory-fixation-compared-to-suspensory-fixation-for-single-bundle-hamstring-acl-reconstruction
#3
JOURNAL ARTICLE
Sam James Daniel, Jozy Timothy, Madhavi Kandagaddala, K Reka, Pradeep Mathew Poonnoose, Anil Thomas Oommen
BACKGROUND: Tunnel widening after Anterior cruciate ligament (ACL) reconstruction using a hamstring graft is known to occur at follow-up. Our study aimed to investigate the effect of suspensory fixation augmentation using an interference screw on tunnel widening in single-bundle hamstring ACL reconstruction. METHODS: 48 patients who had single bundle ACL reconstruction with femoral fixed loop fixation technique in 15 knees, and, fixed loop with augmented aperture (bio screw) fixation in 33 knees were analyzed...
January 2024: Journal of Clinical Orthopaedics and Trauma
https://read.qxmd.com/read/38274071/the-minimum-protein-staple-towards-bio-baldwin-s-rules-via-inter-phosphosite-linking-in-the-mek1-activation-loop
#4
JOURNAL ARTICLE
Sébastien R G Galan, Ritu Raj, Dimitrios Mamalis, Lyn H Jones, Shabaz Mohammed, Benjamin G Davis
In small molecule organic chemistry, the heuristic insight into ring-forming processes that was enabled by Baldwin's rules some 50 years ago proved a step-change in the role of mechanistically guided synthesis. It created a lens upon and marker of fundamental stereoelectronic and conformation-guided chemical processes. However, despite the widespread role of stereoelectronics and conformational control in Biology, no equivalent coherent exploitation of trapped, ring-forming processes yet exists in biomolecules...
January 24, 2024: Chemical Science
https://read.qxmd.com/read/38145749/selective-microbial-production-of-lacto-n-fucopentaose-i-in-escherichia-coli-using-engineered-%C3%AE-1-2-fucosyltransferases
#5
JOURNAL ARTICLE
Shun Endo, Tomotoshi Sugita, Sayaka Kamai, Kazuki Nakamura, Fuhito Yamazaki, Sotaro Sampei, Gustautas Snarskis, Audronė Valančiūtė, Masoud Kazemi, Irmantas Rokaitis, Kento Koketsu
Lacto-N-fucopentaose I (LNFP I) is the second most abundant fucosylated human milk oligosaccharide (HMO) in breast milk after 2'-fucosyllactose (2'-FL). Studies have reported that LNFP I exhibits antimicrobial activity against group B Streptococcus and antiviral effects against Enterovirus and Norovirus. Microbial production of HMOs by engineered Escherichia coli is an attractive, low-cost process, but few studies have investigated production of long-chain HMOs, including the pentasaccharide LNFP I. LNFP I is synthesized by α1,2-fucosyltransfer reaction to the N-acetylglucosamine moiety of the lacto-N-tetraose skeleton, which is catalyzed by α1,2-fucosyltransferase (α1,2-FucT)...
December 23, 2023: Metabolic Engineering
https://read.qxmd.com/read/38056700/investigator-attitudes-on-equipoise-and-practice-patterns-in-the-best-cli-trial
#6
JOURNAL ARTICLE
Alik Farber, Jeffrey J Siracuse, Kristina Giles, Doug Jones, Igor A Laskowski, Richard J Powell, Kenneth Rosenfield, Michael B Strong, Christopher J White, Gheorghe Doros, Matthew T Menard
OBJECTIVES: There has been significant variability in practice patterns and equipoise regarding treatment approach for chronic limb threatening ischemia (CLTI). We aimed to assess treatment preferences of BEST-CLI investigators prior to and following the trial. METHODS: An electronic 60 question survey was sent to 1180 BEST-CLI investigators in 2022 after trial conclusion and before announcement of results. Investigators' preferences were assessed across clinical scenarios for both open (OPEN) and endovascular (ENDO) revascularization strategies...
December 4, 2023: Journal of Vascular Surgery
https://read.qxmd.com/read/38046502/colonic-lipoma-a-rare-cause-of-intussusception
#7
Manjeet K Goyal, Yogesh K Gupta, Varun Mehta, Arshdeep Singh, Ajit Sood
The most common and challenging chief complaint in the emergency department is abdominal pain. Intussusception, although rare in adults, is an important etiology to consider. The diagnosis is often delayed because of the nonspecific symptoms, especially in adults. This case highlights a rare case of intussusception in a middle-aged male with a colonic lipoma as a leading point. Endo-loop was applied to the colonic lipoma, leading to the resolution of intussusception. Therefore, this can be an effective alternative to surgery in select cases...
October 2023: Curēus
https://read.qxmd.com/read/38022040/eustachian-valve-enhanced-paradoxical-cerebral-embolism-a-case-report
#8
Kei Nozue, Hajime Ikenouchi, Tatsuo Miyamoto, Naoki Yamamoto, Kaoru Endo
Cryptogenic stroke includes many suspicious embolic causes that do not fulfill the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification criteria. Atrial fibrillation (AF) is one of the major hidden causes of cryptogenic stroke, and an implantable loop recorder (ILR) is widely used for detecting AF. Herein, we report a case of paradoxical cerebral embolism due to a large Eustachian valve with large PFO under no molecular complete remission (CR) of acute monocytic leukemia (AMoL). A 75-year-old man arrived at our emergency room because of aphasia and right hemiparesis...
October 2023: Curēus
https://read.qxmd.com/read/37999949/circular-business-model-for-digital-health-solutions-protocol-for-a-scoping-review
#9
JOURNAL ARTICLE
Camille Rønn, Andreas Wieland, Christiane Lehrer, Attila Márton, Jason LaRoche, Adrien Specker, Pascal Leroy, Daniel Fürstenau
BACKGROUND: The circular economy reshapes the linear "take, make, and dispose" approach and evolves around minimizing waste and recapturing resources in a closed-loop system. The health sector accounts for 4.6% of global greenhouse gas emissions and has, over the decades, been built to rely on single-use devices and deal with high volumes of medical waste. With the increase in the adoption of digital health solutions in the health care industry, leading the industry into a new paradigm of how we provide health care, a focus must be put on the amount of waste that will follow...
November 24, 2023: JMIR Research Protocols
https://read.qxmd.com/read/37954375/counter-attack-of-biocontrol-agents-environmentally-benign-approaches-against-root-knot-nematodes-meloidogyne-spp-on-agricultural-crops
#10
REVIEW
Amir Khan, Mohammad Haris, Touseef Hussain, Abrar Ahmad Khan, Salah-Eddine Laasli, Rachid Lahlali, Fouad Mokrini
Root-knot nematodes ( Meloidogyne spp.) are obligate sedentary endoparasites, considered severe crop-damaging taxa among all plant-parasitic nematodes globally. Their attacks through parasitic proteins alter the physiology and machinery of the host cells to favour parasitism and reduction in crop yield. Currently, the use of excessive pesticides as a fast remedy to manage this pest is hazardous for both the environment and humans. Keeping this view in mind, there is an urgent need for developing efficient eco-friendly strategies...
November 2023: Heliyon
https://read.qxmd.com/read/37945815/microrna-205-represses-breast-cancer-metastasis-by-perturbing-the-rab-coupling-protein-rcp-mediated-integrin-%C3%AE-1-recycling-on-the-membrane
#11
JOURNAL ARTICLE
Saurav Bhattacharya, Sushmita Sarker, Shaswati Das, Manisha Ahir, Sreya Chattopadhyay, Swatilekha Ghosh, Arghya Adhikary
During cancer cell invasion, integrin undergoes constant endo/exocytic trafficking. It has been found that the recycling ability of integrin β1 through Rab11-controlled long loop pathways is directly associated with cancer invasion. Previous studies showed that gain-of-function mutant p53 regulates the Rab-coupling protein [RCP]-mediated integrin β1 recycling by inactivating tumor suppressor TAp63. So, we were interested to investigate the involvement of miR-205 in this process. In the current study first, we evaluated that the lower expression of miR-205 in MDA-MB-231 cell line is associated with high motility and invasiveness...
November 9, 2023: Apoptosis: An International Journal on Programmed Cell Death
https://read.qxmd.com/read/37764241/structural-expansion-of-catalytic-rna-nanostructures-through-oligomerization-of-a-cyclic-trimer-of-engineered-ribozymes
#12
JOURNAL ARTICLE
Mst Ayesha Siddika, Hiroki Oi, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with simpler, smaller structures serving as units for larger quaternary structures. In this study, we exploited oligomerization of a ribozyme cyclic trimer to achieve larger ribozyme-based RNA assembly. By installing kissing loop (KL) interacting units to one-, two-, or three-unit RNA molecules in the ribozyme trimer, we constructed dimers, open-chain oligomers, and branched oligomers of ribozyme trimer units...
September 6, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/37756004/antifungal-activity-of-ageritin-a-ribotoxin-like-protein-from-cyclocybe-aegerita-edible-mushroom-against-phytopathogenic-fungi
#13
JOURNAL ARTICLE
Sara Ragucci, Stefany Castaldi, Nicola Landi, Rachele Isticato, Antimo Di Maro
Ageritin from poplar mushrooms is a specific endonuclease that hydrolyzes a single phosphodiester bond located in the sarcin-ricin loop (SRL) of the large rRNA, thereby blocking protein synthesis. Considering the possible biotechnological use of this enzyme, here we report its antifungal activity against virulent fungi affecting crops of economic interest. Our results show that ageritin (200 µg/plug; ~13.5 nmole) inhibits the growth of Botrytis cinerea (57%), Colletotrichum truncatum (42%), and Alternaria alternata (57%), when tested on potato dextrose agar plates...
September 18, 2023: Toxins
https://read.qxmd.com/read/37496572/end-sigmoid-colostomy-reconstruction-using-purse-string-subcutaneous-suture-via-extraperitoneal-route-at-the-site-of-a-previous-transperitoneal-loop-stoma
#14
JOURNAL ARTICLE
Kazuhiko Yoshimatsu, Hironori Tanaka, Yoshitomo Ito, Seiya Kinoshita, Masaharu Higashida, Toshimasa Okada, Shuji Endo, Yoshinori Fujiwara, Tomio Ueno
We previously experienced two cases of end sigmoid colostomy reconstruction via the extraperitoneal route at the same site as the transperitoneal loop stoma. For an anterior rectus fascia, the transperitoneal route used closed intraperitoneal interrupted sutures and continuous sutures with barbed sutures. A new extraperitoneal route was established through the sutured anterior rectus sheath. Before reconstructing the end stoma, a subcutaneous purse-string with monofilament absorbable sutures tied to create an approximately 2...
2023: Journal of the Anus, Rectum and Colon
https://read.qxmd.com/read/37284454/hierarchically-tumor-activated-nanocrispr-cas13a-facilitates-efficient-microrna-disruption-for-multi-pathway-mediated-tumor-suppression
#15
JOURNAL ARTICLE
Xiaowei Liu, Suleixin Yang, Li Wang, Xinyue Wu, Xinxin Wang, Chunqing Ou, Jin Yang, Linjiang Song, Shiyao Zhou, Qinjie Wu, Changyang Gong
Rationale: CRISPR-Cas13a is an efficient tool for robust RNA knockdown with lower off-target effect, which may be a potentially powerful and safe tool for cancer gene therapy. However, therapeutic effect of current cancer gene therapy that targeting monogene was compromised by the multi-mutational signal pathway alterations of tumorigenesis. Methods: Here, hierarchically tumor-activated nanoCRISPR-Cas13a (CHAIN) is fabricated for multi-pathway-mediated tumor suppression by efficient microRNA disruption in vivo ...
2023: Theranostics
https://read.qxmd.com/read/37280481/successful-treatment-of-postoperative-nonobstructive-recurrent-cholangitis-by-tract-conversion-surgery-after-total-pancreatectomy-a-case-report
#16
JOURNAL ARTICLE
Masanori Odaira, Fumiki Toriumi, Shota Hoshino, Nozomi Iwama, Yasuhiro Ito, Takashi Endo, Hirohisa Harada
BACKGROUND: Postoperative cholangitis is a complication of biliary reconstruction during hepatobiliary pancreatic surgery. Most cases are associated with anastomotic stenosis, but there are also cases of cholangitis without stenosis, and treatment can be difficult, especially in patients with recurrent symptoms. In this report, we describe a case of repeated nonobstructive cholangitis in a patient after total pancreatectomy, in which a good outcome was obtained after performing tract conversion surgery...
June 7, 2023: Surgical Case Reports
https://read.qxmd.com/read/37265928/loop-technique-for-guidewire-manipulation-during-endoscopic-ultrasound-guided-hepaticogastrostomy
#17
JOURNAL ARTICLE
Haruo Miwa, Kazuya Sugimori, Yuto Matsuoka, Kazuki Endo, Ritsuko Oishi, Masaki Nishimura, Yuichiro Tozuka, Takashi Kaneko, Kazushi Numata, Shin Maeda
BACKGROUND AND AIM: Endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) is widely used in the management of biliary obstructions; however, literature on guidewire manipulation is lacking. This study aimed to assess the utility and optimal conditions of the loop technique for guidewire manipulation during EUS-HGS. METHODS: Consecutive patients who underwent EUS-HGS between April 2015 and January 2022 were included in this study. Patient characteristics and procedural details were retrospectively analyzed...
May 2023: JGH Open: An Open Access Journal of Gastroenterology and Hepatology
https://read.qxmd.com/read/37264672/-57-fe-m%C3%A3-ssbauer-spectroscopy-and-high-pressure-structural-analysis-for-the-mechanism-of-pressure-induced-unique-magnetic-behaviour-in-cation-fe-ii-fe-iii-dto-3-cation-ph-4-p-and-n-prph-3-p-dto-1-2-dithiooxalato
#18
JOURNAL ARTICLE
Ryosuke Taniai, Tsubasa Endo, Takuya Kanetomo, Atsushi Okazawa, Hirokazu Kadobayashi, Saori I Kawaguchi, Masaya Enomoto
A mixed-valence iron(II,III) coordination polymer, (Ph4 P)[FeII FeIII (dto)3 ] (2; Ph4 P = tetraphenylphosphonium, dto = 1,2-dithiooxalato), exhibits a thermal hysteresis loop and a low temperature shift of the ferromagnetic phase transition temperature, with increasing pressure. The latter magnetic behaviour can also be observed in a novel compound ( n PrPh3 P)[FeII FeIII (dto)3 ] (3; n PrPh3 P = n -propyltriphenylphosphonium). To understand the structural information under pressure, we performed high-pressure powder X-ray diffraction, and the result suggests that there was no structural phase transition for either compound...
June 2, 2023: Dalton Transactions: An International Journal of Inorganic Chemistry
https://read.qxmd.com/read/37253081/liquid-metal-nanoplatform-based-autologous-cancer-vaccines
#19
JOURNAL ARTICLE
Dawei Wang, Zhongyang Yu, Yuxia Qi, Kaiwen Hu, Tian Zhou, Jing Liu, Wei Rao
Therapeutic cancer vaccines have been vigorously sought to bolster host adaptive immunity against metastatic cancers, but tumor heterogeneity, ineffective antigen utilization, and immunosuppressive tumor microenvironment hinder their clinical applications. Autologous antigen adsorbability and stimulus-release carrier coupling with immunoadjuvant capacity are urgent for personalized cancer vaccines. Here, we propose a perspective strategy of using a multipotent gallium-based liquid metal (LM) nanoplatform for personalized in situ cancer vaccines (ISCVs)...
May 30, 2023: ACS Nano
https://read.qxmd.com/read/37193606/identification-of-a-novel-enhancer-essential-for-satb1-expression-in-t-h-2-cells-and-activated-ilc2s
#20
JOURNAL ARTICLE
Aneela Nomura, Tetsuro Kobayashi, Wooseok Seo, Michiko Ohno-Oishi, Kiyokazu Kakugawa, Sawako Muroi, Hideyuki Yoshida, Takaho A Endo, Kazuyo Moro, Ichiro Taniuchi
The genome organizer, special AT-rich binding protein-1 (SATB1), functions to globally regulate gene networks during primary T cell development and plays a pivotal role in lineage specification in CD4+ helper-, CD8+ cytotoxic-, and FOXP3+ regulatory-T cell subsets. However, it remains unclear how Satb1 gene expression is controlled, particularly in effector T cell function. Here, by using a novel reporter mouse strain expressing SATB1-Venus and genome editing, we have identified a cis -regulatory enhancer, essential for maintaining Satb1 expression specifically in TH 2 cells...
August 2023: Life Science Alliance
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