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DNA intercalation

Anuj Kumar, Vinod Kumar Vashistha, Prashant Tevatia, Randhir Singh
Tetraazamacrocyclic complexes of Mn(II), Fe(III), Co(II) and Ni(II) have been synthesized by template method. These tetraazamacrocycles have been analyzed with various techniques like molar conductance, IR, UV-vis, mass spectral and cyclic voltammetric studies. On the basis of all these studies, octahedral geometry has been assigned to these tetraazamacrocyclic complexes. The DNA binding properties of these macrocyclic complexes have been investigated by electronic absorption spectra, fluorescence spectra, cyclic voltammetric and differential pulse voltammetric studies...
January 6, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Milan M Milutinović, Ana Rilak, Ioannis Bratsos, Olivera Klisurić, Milan Vraneš, Nevenka Gligorijević, Siniša Radulović, Živadin D Bugarčić
In this study, we have developed a series of new monofunctional Ru(II) complexes of the general formula mer-[Ru(Cl-Ph-tpy)(N-N)Cl]Cl in which Cl-Ph-tpy is 4'-(4-chlorophenyl)-2,2':6',2″-terpyridine, N-N is a bidentate chelating ligand (1,2-diaminoethane (en, 1), 1,2-diaminocyclohexane (dach, 2) or 2,2'-bipyridine (bpy, 3)). All complexes were fully characterized by elemental analysis and spectroscopic techniques (IR, UV-Vis, 1D and 2D NMR). Their chemical behavior in aqueous solution was studied by UV-Vis and NMR spectroscopy showing that all compounds are relatively labile leading to the formation of the corresponding aqua species 1aq-3aq...
October 14, 2016: Journal of Inorganic Biochemistry
Zelal Adiguzel, Seniz Ozalp-Yaman, Gokalp Celik, Safia Salem, Tugba Bagci-Onder, Filiz Senbabaoglu, Yüksel Cetin, Ceyda Acilan
Here, we describe the characteristics of a Pt-blue complex [Pt4 (2-atp)8 (H2 O)(OH)] (2-atp: 2-aminothiophenol) as a prodrug for its DNA-binding properties and its use in cancer therapy. The nature of the interaction between the Pt-blue complex and DNA was evaluated based on spectroscopic measurements, the electronic absorption spectra, thermal behavior, viscosity, fluorometric titration and agarose gel electrophoresis. Our results suggested that the compound was able to partially intercalate DNA and appeared to induce both single and double stranded breaks (DBS) on DNA in vitro, but no DSBs in cells...
January 12, 2017: Chemical Biology & Drug Design
Anjali Soni, Pooja Khurana, Tanya Singh, B Jayaram
MOTIVATION: Drug intercalation is an important strategy for DNA inhibition which is often employed in cancer chemotherapy. Despite its high significance, the field is characterized by limited success in identification of novel intercalator molecules and lack of automated and dedicated drug-DNA intercalation methodology. RESULTS: We report here a novel intercalation methodology (christened 'Intercalate') for predicting both the structures and energetics of DNA-intercalator complexes, covering the processes of DNA unwinding and (non-covalent) binding...
January 10, 2017: Bioinformatics
Hiwa Saeed, Ibrahim Saeed, Niklaas Buurma, Jim Antony Thomas
With the long-term aim of enhancing the binding properties of dinuclear RuII-based DNA light-switch complexes, a series of eight structurally related mono- and dinuclear systems are reported in which the linker of the bridging ligand has been modulated. These tethered systems have been designed to explore issues of steric demand at the binding site and the thermodynamic cost of entropy loss upon binding. An analysis of detailed spectroscopic and isothermal titration calorimetry (ITC) studies on the new complexes reveal that one of the linkers produces a dinuclear systems that binds to duplex DNA with an affinity (Kb > 107 M-1) that is higher than its corresponding monometallic complex and is the highest affinity for a non-threading bis-intercalating metal complex...
January 10, 2017: Chemistry: a European Journal
Tamsyn A Hilder, Justin M Hodgkiss
Although 17β-estradiol (E2) is a natural molecule involved in the endocrine system, its widespread use in various applications has resulted in its accumulation in the environment and its classification as an endocrine-disrupting molecule. These molecules can interfere with the hormonal system, and have been linked to various adverse effects such as the proliferation of breast cancer. It has been proposed that E2 could contribute to breast cancer by the induction of DNA damage. Mass spectrometry has demonstrated that E2 can bind to DNA but the mechanism by which E2 interacts with DNA has yet to be elucidated...
2017: Computational and Structural Biotechnology Journal
Lidia V Starostenko, Nadejda I Rechkunova, Natalia A Lebedeva, Alexander A Lomzov, Vladimir V Koval, Olga I Lavrik
The major enzyme in eukaryotic cells that catalyzes the cleavage of apurinic/apyrimidinic (AP or abasic) sites is AP endonuclease 1 (APE1) that cleaves the phosphodiester bond on the 5'-side of AP sites. We found that the efficiency of AP site cleavage by APE1 was affected by the benzo[a]pyrenyl-DNA adduct (BPDE-dG) in the opposite strand. AP sites directly opposite of the modified dG or shifted toward the 5' direction were hydrolyzed by APE1 with an efficiency moderately lower than the AP site in the control DNA duplex, whereas AP sites shifted toward the 3' direction were hydrolyzed significantly less efficiently...
December 27, 2016: DNA Repair
Nilesh Umakant Deshpande, Manickam Jayakannan
New cisplatin-stitched polysaccharide vesicular nanocarrier is developed for combination therapy of three clinical important antagonistic drugs together to accomplish synergistic cancer therapy in breast cancer treatment. Carboxylic functionalized dextran was tailor-made for the chemical conjugation of cisplatin, and a renewable hydrophobic unit was anchored in the backbone to interdigitize the chains to self-assemble as cisplatin-stitched polysaccharide nanovesicles. Water-soluble DNA-intercalating drug doxorubicin·HCl (DOX) and water insoluble topoisomerase type I inhibitor drug camptothecin (CPT) were encapsulated in these vesicles to produce dual or triple drug-loaded vesicular nanocarrier...
January 9, 2017: Biomacromolecules
Akihito Shimazu, Masashi Kawagoshi, Shoichi Takeda, Haruaki Kurasaki, Asako Kato, Nahoko Morii, Norio Sakai, Takeo Konakahara
The binding modes and binding constants for the complexes of forty types of pyridocarbazole derivatives 1-40 with double stranded DNAs (dsDNAs) were reported. The binding modes were determined by a combination of a deflection spectroscopy and orientation of the corresponding molecule in the DNA-based film with chain alignment. All of the compounds exhibited the intercalation-binding mode. Its binding constants Ka for the complexes, determined by quartz crystal microbalance (QCM), varied from 1.7×10(5) to 4...
December 27, 2016: Bioorganic & Medicinal Chemistry
Daniela Iannazzo, Alessandro Pistone, Marina Salamò, Signorino Galvagno, Roberto Romeo, Salvatore V Giofré, Caterina Branca, Giuseppa Visalli, Angela Di Pietro
A biocompatible and cell traceable drug delivery system Graphene Quantum Dots (GQD) based, for the targeted delivery of the DNA intercalating drug doxorubicin (DOX) to cancer cells, is here reported. Highly dispersible and water soluble GQD, synthesized by acidic oxidation and exfoliation of multi-walled carbon nanotubes (MWCNT), were covalently linked to the tumor targeting module biotin (BTN), able to efficiently recognize biotin receptors over-expressed on cancer cells and loaded with DOX. Biological test performed on A549 cells reported a very low toxicity of the synthesized carrier (GQD and GQD-BTN)...
January 2, 2017: International Journal of Pharmaceutics
M P Kesavan, G G Vinoth Kumar, J Dhaveethu Raja, K Anitha, S Karthikeyan, J Rajesh
Luotonin A (L), a novel natural cytotoxic and anti-inflammatory alkaloid, chelated with copper(II) to improve its cytotoxic effect against the cancer cells. The complexes [Cu(L)H2OCl]Cl (1) and [Cu(L)2]Cl2 (2) are prepared by using copper(II) chloride and L with ligand/metal molar ratio of 1:1 and 2:1 respectively. A solution of complexes 1 &2 are characterized by physical spectroscopic methods using Ultraviolet-visible (UV-Vis) spectrophotometer, Fourier Transform-Infra red (FT-IR) spectroscopy, Electron Para magnetic Resonance Spectroscopy (EPR) and by electrochemical methods...
December 22, 2016: Journal of Photochemistry and Photobiology. B, Biology
Dongye He, Lili Wang, Liping Wang, Xiaoyu Li, Yongping Xu
The types of interactions between six novel bisnaphthalimide derivatives (AITHN, BITHN, PITHN, PyITHN, DN6 and DNT6) and calf thymus DNA in a physiological buffer (Tris-HCl buffer solutions, pH=7.4) were investigated using UV-vis spectrophotometry, fluorescence spectroscopy, and a competition experiment, in order to explore the relationships between the linkers of bisnaphthalimide derivatives and their activity. The absorption spectra of the six bisnaphthalimide derivatives with DNA showed a slight red shift and hypochromic effect...
January 2017: Journal of Photochemistry and Photobiology. B, Biology
Shizuka Nakayama, Jie Zhou, Yue Zheng, Henryk Szmacinski, Herman O Sintim
BACKGROUND: Cyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine. METHODS & RESULTS: Atomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA...
March 2016: Future Science OA
Aysegul Kutluay Baytak, Tugce Teker, Sehriban Duzmen, Mehmet Aslanoglu
A composite of carbon nanofibers (CNFs) and tri-metallic nanoparticles of gold, cobalt and nickel were used for the preparation of a novel voltammetric platform. The proposed voltammetric platform was utilized for quantifying ethyl paraben (EPB) in pharmaceutical and cosmetic products. The electrode layers were characterized by utilizing X-ray diffraction method (XRD) and Fourier transform infrared spectroscopy (FTIR). The electrode system, (Au-Ni-Co)NPs-CNFs/GCE, exhibited high catalytic activity and enhanced the electrochemical behaviour of EPB compared with several other electrodes...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Martin R Gill, Siti Norain Harun, Swagata Halder, Ramon A Boghozian, Kristijan Ramadan, Haslina Ahmad, Katherine A Vallis
No abstract text is available yet for this article.
December 22, 2016: Scientific Reports
Adeline Huiling Loo, Chun Kiang Chua, Martin Pumera
3D printing, an upcoming technology, has vast potential to transform conventional fabrication processes due to the numerous improvements it can offer to the current methods. To date, the employment of 3D printing technology has been examined for applications in the fields of engineering, manufacturing and biological sciences. In this study, we examined the potential of adopting 3D printing technology for a novel application, electrochemical DNA biosensing. Metal 3D printing was utilized to construct helical-shaped stainless steel electrodes which functioned as a transducing platform for the detection of DNA hybridization...
December 21, 2016: Analyst
Wojciech Streciwilk, Alessio Terenzi, Rainer Misgeld, Corazon Frias, Peter G Jones, Aram Prokop, Bernhard Keppler, Ingo Ott
Naphthalimide-based N-heterocyclic carbene (NHC) complexes of the type [(COD)(NHC)RhCl) (4a-c), [(p-cymene)(NHC)RuCl2) (5a-c) and [(NHC)CuBr] (6a-c) were synthesised and investigated as antiproliferative agents that target DNA. The cytotoxic effects were largely driven by the naphthalimide structure, which represents a DNA intercalating moiety. Regarding the metal centre, the highest activities were observed with the rhodium complexes and the cytotoxic activity was strongly decreased for the ruthenium derivatives...
December 20, 2016: ChemMedChem
Paul Carini, Patrick J Marsden, Jonathan W Leff, Emily E Morgan, Michael S Strickland, Noah Fierer
Extracellular DNA from dead microorganisms can persist in soil for weeks to years(1-3). Although it is implicitly assumed that the microbial DNA recovered from soil predominantly represents intact cells, it is unclear how extracellular DNA affects molecular analyses of microbial diversity. We examined a wide range of soils using viability PCR based on the photoreactive DNA-intercalating dye propidium monoazide(4). We found that, on average, 40% of both prokaryotic and fungal DNA was extracellular or from cells that were no longer intact...
December 19, 2016: Nature Microbiology
Aun Raza, Shumaila Bano, Xiuquan Xu, Rong Xian Zhang, Haider Khalid, Furqan Muhammad Iqbal, Changkun Xia, Jian Tang, Zhen Ouyang
The rutin-nickel (II) complex (RN) was synthesized and characterized by elemental analysis, UV-visible spectroscopy, IR, mass spectrometry, (1)H NMR, TG-DSC, SEM, and molar conductivity. The low molar conductivity value investigates the non-electrolyte nature of the complex. The elemental analysis and other physical and spectroscopic methods reveal the 1:2 stoichiometric ratio (metal/ligand) of the complex. An antioxidant study of rutin and its metal complex against DPPH radical showed that the complex has more radical scavenging activity than free rutin...
December 17, 2016: Biological Trace Element Research
Qi Luo, Qiaomei Jin, Chang Su, Dongjian Zhang, Cuihua Jiang, Anne Folta Fish, Yuanbo Feng, Yicheng Ni, Jian Zhang, Zhiqi Yin
A rapid and accurate identification of necrotic myocardium is of great importance for diagnosis, risk stratification, clinical decision-making, and prognosis evaluation of myocardial infarction. Here we explored technetium-99m labeled rhein derivatives for rapid imaging of the necrotic myocardium. Three hydrazinonicotinic acid-linker-rhein (HYNIC-linker-rhein) derivatives were synthesised and then these synthetic compounds were labeled with technetium-99m using ethylenediaminediacetic acid (EDDA) and tricine as coligands [99mTc(EDDA)-HYNIC-linker-rhein]...
December 16, 2016: Analytical Chemistry
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