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DNA intercalation

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https://www.readbyqxmd.com/read/28431881/design-and-synthesis-of-1-2-3-triazolo-phenanthrene-hybrids-as-cytotoxic-agents
#1
Niggula Praveen Kumar, Shalini Nekkanti, S Sujana Kumari, Pankaj Sharma, Nagula Shankaraiah
A series of new 1,2,3-triazolo-phenanthrene hybrids has been synthesized by employing Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. These compounds were evaluated for their in vitro cytotoxic potential against various human cancer cell lines viz. lung (A549), prostate (PC-3 and DU145), gastric (HGC-27), cervical (HeLa), triple negative breast (MDA-MB-231, MDA-MB-453) and breast (BT-549, 4T1) cells. Among the tested compounds, 7d displayed highest cytotoxicity against DU145 cells with IC50 value of 1...
April 7, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28430282/a-ratiometric-sensor-for-dna-based-on-a-dual-emission-ru-dppz-light-switch-complex
#2
Michael G Walker, Vadde Ramu, Anthony J H M Meijer, Amitava Das, Jim A Thomas
Herein we describe the DNA binding properties of two new water-soluble ruthenium complexes; experimental and computational data reveal that both complexes display dual emission from MLCT and LLCT excited states. The interaction of the new complexes with DNA was also investigated. Although one of the complexes only binds DNA though groove binding, the second complex has separate ligands capable of groove binding and intercalation. Nevertheless, it was found that both complexes interact with duplex DNA with high affinity...
April 21, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28426082/deciphering-of-interactions-between-platinated-dna-and-hmgb1-by-hydrogen-deuterium-exchange-mass-spectrometry
#3
Yuanyuan Wang, Zhifeng Du, Wei Zheng, Kui Wu, Decheng Xu, Qun Luo, Yao Zhao, Juanjuan Han, Yangzhong Liu, Fuyi Wang
A high mobility group box 1 (HMGB1) protein has been reported to recognize both 1,2-intrastrand crosslinked DNA by cisplatin (1,2-cis-Pt-DNA) and monofunctional platinated DNA using trans-[PtCl2(NH3)(thiazole)] (1-trans-PtTz-DNA). However, the molecular basis of recognition between the trans-PtTz-DNA and HMGB1 remains unclear. In the present work, we described a hydrogen/deuterium exchange mass spectrometry (HDX-MS) method in combination with docking simulation to decipher the interactions of platinated DNA with domain A of HMGB1...
April 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28425525/exploiting-hydrogen-bonding-interactions-to-probe-smaller-linear-and-cyclic-diamines-binding-to-g-quadruplexes-a-dft-and-molecular-dynamics-study
#4
Mrinal Kanti Si, Anik Sen, Bishwajit Ganguly
G-quadruplexes are formed by the association of four guanine bases through Hoogsteen hydrogen bonding in guanine-rich sequences of DNA and exist in the telomere as well as in promoter regions of certain oncogenes. The sequences of G-quadruplex-DNA are targets for the design of molecules that can bind and can be developed as anti-cancer drugs. The linear and cyclic protonated diamines have been explored to bind to G-quadruplex-DNA through hydrogen bonding interactions. The quadruplex-DNA binders exploit π-stacking and hydrogen bonding interactions with the phosphate backbone of loops and grooves...
April 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28421985/design-of-a-sensitive-and-selective-electrochemical-aptasensor-for-the-determination-of-the-complementary-cdna-of-mirna-145-based-on-the-intercalation-and-electrochemical-reduction-of-doxorubicin
#5
Maryam Mohamadi, Ali Mostafavi, Masoud Torkzadeh-Mahani
<p>The aim of this research was the determination of a microRNA (miRNA) using a DNA electrochemical aptasensor. In this biosensor, the complementary complementary DNA (cDNA) of miRNA-145 (a sense RNA transcript) was the target strand and the cDNA of miRNA-145 was the probe strand. Both cDNAs can be the product of the reverse transcriptase-polymerase chain reaction of miRNA. The proposed aptasensor's function was based on the hybridization of target strands with probes immobilized on the surface of a working electrode and the subsequent intercalation of doxorubicin (DOX) molecules functioning as the electroactive indicators of any double strands that formed...
April 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28417283/bis-anthracycline-wp760-abrogates-melanoma-cell-growth-by-transcription-inhibition-p53-activation-and-igf1r-downregulation
#6
Magdalena Olbryt, Aleksandra Rusin, Izabela Fokt, Anna Habryka, Patrycja Tudrej, Sebastian Student, Aleksander Sochanik, Rafał Zieliński, Waldemar Priebe
Anthracycline chemotherapeutics, e.g. doxorubicin and daunorubicin, are active against a broad spectrum of cancers. Their cytotoxicity is mainly attributed to DNA intercalation, interference with topoisomerase activity, and induction of double-stranded DNA breaks. Since modification of anthracyclines can profoundly affect their pharmacological properties we attempted to elucidate the mechanism of action, and identify possible molecular targets, of bis-anthracycline WP760 which previously demonstrated anti-melanoma activity at low nanomolar concentrations...
April 17, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28415741/enhancement-of-radiosensitivity-by-the-novel-anticancer-quinolone-derivative-vosaroxin-in-preclinical-glioblastoma-models
#7
Giovanni Luca Gravina, Andrea Mancini, Claudia Mattei, Flora Vitale, Francesco Marampon, Alessandro Colapietro, Giulia Rossi, Luca Ventura, Antonella Vetuschi, Ernesto Di Cesare, Judith A Fox, Claudio Festuccia
PURPOSE: Glioblastoma multiforme (GBM) is the most aggressive brain tumor. The activity of vosaroxin, a first-in-class anticancer quinolone derivative that intercalates DNA and inhibits topoisomerase II, was investigated in GBM preclinical models as a single agent and combined with radiotherapy (RT). RESULTS: Vosaroxin showed antitumor activity in clonogenic survival assays, with IC50 of 10-100 nM, and demonstrated radiosensitization. Combined treatments exhibited significantly higher γH2Ax levels compared with controls...
March 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415566/co-targeting-of-dna-rna-and-protein-molecules-provides-optimal-outcomes-for-treating-osteosarcoma-and-pulmonary-metastasis-in-spontaneous-and-experimental-metastasis-mouse-models
#8
Chao Jian, Mei-Juan Tu, Pui Yan Ho, Zhijian Duan, Qianyu Zhang, Jing-Xin Qiu, Ralph W DeVere White, Theodore Wun, Primo N Lara, Kit S Lam, Ai-Xi Yu, Ai-Ming Yu
Metastasis is a major cause of mortality for cancer patients and remains as the greatest challenge in cancer therapy. Driven by multiple factors, metastasis may not be controlled by the inhibition of single target. This study was aimed at assessing the hypothesis that drugs could be rationally combined to co-target critical DNA, RNA and protein molecules to achieve "saturation attack" against metastasis. Independent actions of the model drugs DNA-intercalating doxorubicin, RNA-interfering miR-34a and protein-inhibiting sorafenib on DNA replication, RNA translation and protein kinase signaling in highly metastatic, human osteosarcoma 143B cells were demonstrated by the increase of γH2A...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415390/synthesis-spectroscopic-and-dna-binding-ability-of-co-ii-ni-ii-cu-ii-and-zn-ii-complexes-of-schiff-base-ligand-e-1-1h-benzo-d-imidazol-2-yl-methylimino-methyl-naphthalen-2-ol-x-ray-crystal-structure-determination-of-cobalt-ii-complex
#9
Naeema H Yarkandi, Hoda A El-Ghamry, Mohamed Gaber
A novel Schiff base ligand, (E)-1-(((1H-benzo[d]imidazol-2-yl)methylimino)methyl)naphthalen-2-ol (HL), has been designed and synthesized in addition to its metal chelates [Co(L)2]·l2H2O, [Ni(L)Cl·(H2O)2].5H2O, [Cu(L)Cl] and [Zn(L)(CH3COO)]. The structures of the isolated compounds have been confirmed and identified by means of different spectral and physicochemical techniques including CHN analysis, (1)H &(13)C NMR, mass spectral analysis, molar conductivity measurement, UV-Vis, infrared, magnetic moment in addition to TGA technique...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28407412/adsorption-and-desorption-of-single-stranded-dna-from-single-walled-carbon-nanotubes
#10
Cameron James Shearer, LePing Yu, Renzo Fenati, Alex Sibley, Jamie Scott Quinton, Christopher Thomas Gibson, Amanda Vera Ellis, Gunther Andersson, Joseph George Shapter
The chemical affinity of single stranded DNA (ssDNA) to adsorb to the surface of single walled carbon nanotubes (SWCNTs) is used for SWCNT purification, separation and in bio-devices. Despite the popularity of research on SWCNT-ssDNA conjugates, very little work has been dedicated to study the removal of adsorbed ssDNA on SWCNTs. This paper reports a comprehensive study of biological, physical and chemical treatments for the removal of ssDNA from SWCNT-ssDNA suspensions. These include enzymatic cleavage, heat treatment under vacuum up to 400 oC, chemical treatments with high or low pH, oxidising conditions, and high ionic strength solvents...
April 13, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28388047/new-acridine-thiourea-gold-i-anticancer-agents-targeting-the-nucleus-and-inhibiting-vasculogenic-mimicry
#11
Sergio A Pérez, Concepción de Haro, Consuelo Vicente, Antonio Donaire, Ana Zamora, Juraj Zajac, Hana Kostrhunova, Viktor Brabec, Delia Bautista, José Ruiz
Two new 1-acridin-9-yl-3-methylthiourea Au(I) DNA intercalators [Au(ACRTU)2]Cl (2) and [Au(ACRTU) (PPh3)]PF6 (3) have been prepared. Both complexes were highly active in the human ovarian carcinoma cisplatin-sensitive A2780 cell line, exhibiting IC50 values in the submicromolar range. Compounds 2 and 3 are also cytotoxic toward different phenotypes of breast cancer cell lines MDA-MB-231 (triple negative), SK-BR-3 (HER2+, ERα-, and ERβ-), and MCF-7 (ER+). Both complexes induce apoptosis through activation of caspase-3 in vitro...
April 19, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28380010/application-of-jc1-for-non-toxic-isolation-of-cells-with-mdr-transporter-activity-by-flow-cytometry
#12
J Mario Wolosin, Aldo Zamudio, Zheng Wang
The DNA intercalating dye Hoechst 33342 or its close analog DCV are actively removed from cells by the multidrug resistance transporter ABCG2, a protein overexpressed in metastatic cells and somatic stem cells. In bivariate blue-red flow cytometry fluorescent plots active Hoechst or DCV efflux combined with a concentration dependent bathochromic shifts of these nuclear dyes leads to the segregation of the transporter-rich cells into a distinct cell cohort tilted towards the shorter wavelength axis of the plot, the cohort is generically known as the side population (SP)...
2017: PloS One
https://www.readbyqxmd.com/read/28378768/a-novel-label-free-fluorescence-assay-for-one-step-sensitive-detection-of-hg-2-in-environmental-drinking-water-samples
#13
Ya Li, Nan Liu, Hui Liu, Yu Wang, Yuwei Hao, Xinhua Ma, Xiaoli Li, Yapeng Huo, Jiahai Lu, Shuge Tang, Caiqin Wang, Yinhong Zhang, Zhixian Gao
A novel label-free fluorescence assay for detection of Hg(2+) was developed based on the Hg(2+)-binding single-stranded DNA (ssDNA) and SYBR Green I (SG I). Differences from other assays, the designed rich-thymine (T) ssDNA probe without fluorescent labelling can be rapidly formed a T-Hg(2+)-T complex and folded into a stable hairpin structure in the presence of Hg(2+) in environmental drinking water samples by facilitating fluorescence increase through intercalating with SG I in one-step. In the assay, the fluorescence signal can be directly obtained without additional incubation within 1 min...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28377530/topoisomerase-ii-inhibitors-induce-dna-damage-dependent-interferon-responses-circumventing-ebola-virus-immune-evasion
#14
Priya Luthra, Sebastian Aguirre, Benjamin C Yen, Colette A Pietzsch, Maria T Sanchez-Aparicio, Bersabeh Tigabu, Lorraine K Morlock, Adolfo García-Sastre, Daisy W Leung, Noelle S Williams, Ana Fernandez-Sesma, Alexander Bukreyev, Christopher F Basler
Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, identified five DNA intercalators as reproducible hits from a library of bioactive compounds. Four, including doxorubicin and daunorubicin, are anthracycline antibiotics that inhibit topoisomerase II and are used clinically as chemotherapeutic drugs...
April 4, 2017: MBio
https://www.readbyqxmd.com/read/28377493/roles-of-leu28-side-chain-intercalation-in-the-interaction-between-cren7-and-dna
#15
Zhenfeng Zhang, Mohan Zhao, Li Wang, Yuanyuan Chen, Yuhui Dong, Yong Gong, Li Huang
Crenarchaeal chromatin protein Cren7 binds double-stranded DNA in the minor groove, introducing a sharp single-step DNA kink. The side chain of Leu28, a residue conserved among all Cren7 homologues, intercalates into the kink DNA step. In this study, we replaced Leu28 with a residue containing a hydrophobic side chain of different sizes (i.e., L28A, L28V, L28I, L28M and L28F). Both the stability of the Cren7-DNA complex and the ability of Cren7 to constrain DNA supercoils correlated well with the size of the intercalated side chain...
April 4, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28375569/1-8-naphthalimide-a-potent-dna-intercalator-and-target-for-cancer-therapy
#16
REVIEW
Runjhun Tandon, Vijay Luxami, Harsovin Kaur, Nitin Tandon, Kamaldeep Paul
The poor pharmacokinetics, side effects and particularly the rapid emergence of drug resistance compromise the efficiency of clinically used anticancer drugs. Therefore, the discovery of novel and effective drugs is still an extremely primary mission. Naphthalimide family is one of the highly active anticancer drug based upon effective intercalator with DNA. In this article, we review the discovery and development of 1,8-naphthalimide moiety, and, especially, pay much attention to the structural modifications and structure activity relationships...
April 4, 2017: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/28372728/determination-of-cdna-encoding-bcr-abl-fusion-gene-in-patients-with-chronic-myelogenous-leukemia-using-a-novel-fret-based-quantum-dots-dna-nanosensor
#17
Mojtaba Shamsipur, Vahid Nasirian, Ali Barati, Kamran Mansouri, Asad Vaisi-Raygani, Soheila Kashanian
In the present study, we developed a sensitive method based on fluorescence resonance energy transfer (FRET) for the determination of the BCR/ABL fusion gene, which is used as a biomarker to confirm the clinical diagnosis of both chronic myelogenous leukemia (CML) and acute lymphocytic leukemia (ALL). For this purpose, CdTe quantum dots (QDs) were conjugated to amino-modified 18-mer oligonucleotide ((N)DNA) to form the QDs-(N)DNA nanosensor. In the presence of methylene blue (MB) as an intercalator, the hybridization of QDs-(N)DNA with the target BCR/ABL fusion gene (complementary DNA), brings the MB (acceptor) at close proximity of the QDs (donor), leading to FRET upon photoexcitation of the QDs...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28370171/a-review-on-acridinylthioureas-and-its-derivatives-biological-and-cytotoxic-activity
#18
REVIEW
Mária Kožurková, Danica Sabolová, Pavol Kristian
Acridines possess two characteristics that have led many researchers to consider the agents interesting targets for future development as potential farmacophores: the planar acridine skeleton, which is able to intercalate into DNA, and the intense fluorescence of the agents. This review offers a study of the multifunctional character of acridines and the synthesis of novel acridine derivatives, with particular focus being placed on isothiocyanates and their congeners, e.g. thioureas, isothioureas, quaternary ammonium salts and platinum/gold conjugates...
March 31, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28362164/determination-of-restriction-enzyme-activity-when-cutting-dna-labeled-with-the-toto-dye-family
#19
April Maschmann, Kristy L Kounovsky-Shafer
Optical mapping, a single DNA molecule genome analysis platform that can determine methylation profiles, uses fluorescently labeled DNA molecules that are elongated on the surface and digested with a restriction enzyme to produce a barcode of that molecule. Understanding how the cyanine fluorochromes affect enzyme activity can lead to other fluorochromes used in the optical mapping system. The effects of restriction digestion on fluorochrome labeled DNA (Ethidium Bromide, DAPI, H33258, EthD-1, TOTO-1) have been analyzed previously...
March 31, 2017: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/28359905/effects-of-tio2-crystal-structure-on-the-luminescence-quenching-of-ru-bpy-2-dppz-2-intercalated-into-dna
#20
Linlin Chen, Yi Wang, Minggao Huang, Xiaodan Li, Licai Zhu, Hong Li
The intercalation of [Ru(bpy)2(dppz)](2+) labeled as Ru(II) (bpy=2,2'-bipyridine and dppz=dipyrido[3,2,-a:2',3'-c]phenazine) into herring sperm DNA leads to the formation of emissive Ru(II)-DNA dyads, which can be quenched by TiO2 nanoparticles (NPs) and sol-gel silica matrices at heterogeneous interfaces. The calcinations temperature exhibits a remarkable influence on the luminescence quenching of the Ru(II)-DNA dyads by TiO2 NPs. With increasing calcinations temperature in the range from 200 to 850°C, the anatase-to-rutile TiO2 crystal structure transformation increases the average particle size and hydrodynamic diameter of TiO2 and DNA@TiO2...
March 22, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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