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DNA intercalation

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https://www.readbyqxmd.com/read/29338178/dna-damage-and-apoptosis-induction-in-cancer-cells-by-chemically-engineered-thiolated-riboflavin-gold-nanoassembly
#1
Abhishek Sau, Sulagna Sanyal, Kallol Bera, Sabyasachi Sen, Amrit Krishna Mitra, Uttam Pal, Prabal Kumar Chakraborty, Sayantan Ganguly, Biswarup Satpati, Chandrima Das, Samita Basu
Herein we have engineered a smart nuclear targeting thiol-modified riboflavin-gold nano assembly, RfS@AuNPs, which accumulates selectively in nucleus without any nuclear-targeting peptides (NLS/RGD) and shows photophysically in vitro DNA intercalation. A theoretical model using Molecular Dynamics has been developed to probe the mechanism of formation and stability as well as dynamics of the RfS@AuNPs in aqueous solution and within DNA microenvironment. The RfS@AuNPs facilitate the binucleated cell formation that is reflected in the significant increase of DNA damage marker, γ-H2AX as well as the arrest of most of the HeLa cells at pre-G1 phase indicating cell death...
January 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29336569/paper-based-dna-reader-for-visualized-quantification-of-soil-transmitted-helminth-infections
#2
Alex Guan Wang, Tianyu Dong, Hayam Mansour, Gabriela Matamoros, Ana L Sanchez, Feng Li
Soil-transmitted helminth (STH) infections are a global health issue affecting nearly one-third of the world's population. As most endemic areas of STH are impoverished countries or regions with limited healthcare resources, the accurate diagnosis of STH requires analytical tools that are not only quantitative, but also portable, inexpensive, and with no or minimal demand for external instrument. Herein, we introduce a novel paper-based diagnostic device, termed quantitative paper-based DNA reader (qPDR), capable of quantifying STH at the molecular level by measuring distance as readout, thus eliminating the need for external readers...
January 16, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29334166/investigating-the-accessibility-of-nucleosome-by-duocarmycin-b2-fe-ii-peplomycin-and-mnase-using-capillary-electrophoresis
#3
Tingting Zou, Seiichiro Kizaki, Hiroshi Sugiyama
Capillary electrophoresis coupled with DNA 5 Texas Red labeling was used to investigate the ability of duocarmycin B2, Fe(II) peplomycin, and MNase to access the nucleosome. Distinct accessibility patterns of these species to the nucleosome were observed. Duocarmycin B2 was able to enter and react at the core region, although its alkylating efficiency decreased significantly. Intercalation of peplomycin in the nucleosomal core region was highly suppressed, but reaction sites located at the ends of the nucleosomal core remained accessible, which implied the flexibility of the core DNA end...
January 15, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29332793/label-free-detection-of-histone-based-on-cationic-conjugated-polymer-mediated-fluorescence-resonance-energy-transfer
#4
Xiaozhen Lu, Hongxia Jia, Xiaohua Yan, Jingsha Wang, Yucong Wang, Chenghui Liu
A simple and homogeneous histone assay is developed based on histone-induced DNA compressing coupled with cationic conjugated polymer (CCP)-mediated fluorescence resonance energy transfer (FRET). In this strategy, the CCP serves as the FRET donor and SYBR Green I (SG), which can strongly fluoresce not at its free state but after intercalated into the double stranded calf thymus DNA (dsDNA), serves as the acceptor of FRET. In the absence of histone, the dsDNA-SG and CCP combine with each other through electrostatic interaction and the strong FRET from CCP to SG occurs due to the overlapping between the fluorescent emitting spectrum of the CCP and the absorption spectrum of SG...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29321581/a-ruthenium-based-5-fluorouracil-complex-with-enhanced-cytotoxicity-and-apoptosis-induction-action-in-hct116-cells
#5
Valdenizia Rodrigues Silva, Rodrigo S Corrêa, Luciano de Souza Santos, Milena Botelho Pereira Soares, Alzir Azevedo Batista, Daniel Pereira Bezerra
Combination of multifunctionalities into one compound is a rational strategy in medicinal chemical design, and have often been used with metallodrug-based compounds. In the present study, we synthesized a novel ruthenium-based 5-fluorouracil complex [Ru(5-FU)(PPh3)2(bipy)]PF6 (PPh3 = triphenylphosphine; and bipy = 2,2'-bipyridine) with enhanced cytotoxicity in different cancer cells, and assessed its apoptosis induction action in human colon carcinoma HCT116 cells. The complex was characterized by infrared, cyclic voltammetry, molar conductance measurements, elemental analysis, NMR experiments and X-ray crystallographic analysis...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320873/does-progressive-nuclear-staining-with-hemalum-alum-hematoxylin-involve-dna-and-what-is-the-nature-of-the-dye-chromatin-complex
#6
J A Kiernan
Previous investigators have disagreed about whether hemalum stains DNA or its associated nucleoproteins. I review here the literature and describe new experiments in an attempt to resolve the controversy. Hemalum solutions, which contain aluminum ions and hematein, are routinely used to stain nuclei. A solution containing 16 Al3+ ions for each hematein molecule, at pH 2.0-2.5, provides selective progressive staining of chromatin without cytoplasmic or extracellular "background color." Such solutions contain a red cationic dye-metal complex and an excess of Al3+ ions...
January 11, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29317345/muc1-aptamer-targeted-dna-micelles-for-dual-tumor-therapy-using-doxorubicin-and-kla-peptide
#7
Fahimeh Charbgoo, Mona Alibolandi, Seyed Mohammad Taghdisi, Khalil Abnous, Fatemeh Soltani, Mohammad Ramezani
Targeted delivery of DNA nanoparticles is a promising approach in cancer therapy. Using aptamers, target specific delivery of DNA nanoparticles can be achieved. Further, aptamers can indirectly improve drug encapsulation efficiency of DNA nanoparticles for drugs intercalated within nucleic acid base pairs. Using DNA blocks, a micellar hybrid nanoparticle was prepared for the targeted co-delivery of doxorubicin and a pro-apoptotic peptide, KLA to tumor cells. Results demonstrated that anti-MUC1 aptamer could specifically deliver the synthesized DNA micelle into MCF-7 cells by improving its cellular uptake...
January 6, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29315592/identification-of-a-pyranocoumarin-photosensitizer-that-is-a-potent-inhibitor-of-keratinocyte-growth
#8
Jeffrey D Laskin, Yi-Hua Jan, Michele M Jetter, Christophe D Guillon, Thomas M Mariano, Diane E Heck, Ned D Heindel
Photosensitizers are used in the treatment of epidermal proliferation and differentiation disorders such as psoriasis and vitiligo. In the present studies, a ring-expanded carbon homologue of the linear psoralen (furo[3,2-g]benzopyran-7-one) class of photosensitizers, 4,10-dimethyl-2H,8H-benzo[1,2-b:5,4-b']dipyran-2-one (NDH2476), was synthesized and analyzed for biological activity. Following activation by ultraviolet light (UVA, 320-400 nm), NDH2476 was found to be a potent inhibitor of keratinocyte growth (IC50 = 9 nM)...
January 6, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29310253/an-electrochemical-aptasensor-for-the-highly-sensitive-detection-of-8-hydroxy-2-deoxyguanosine-based-on-the-hybridization-chain-reaction
#9
Li-Ping Jia, Li-Juan Wang, Rong-Na Ma, Lei Shang, Wei Zhang, Qing-Wang Xue, Huai-Sheng Wang
In the present work a highly sensitive and selective aptasensor was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) based on the hybridization chain reaction (HCR) signal amplification. It was observed that the aptamer of 8-OH-dG could hybridize with the capture DNA immobilized on the gold electrode with a sticky tail left, which initiated the HCR and led to the formation of extended dsDNA structure on the electrode surface. Then the electroactive species ([Ru(NH3)6]3+, RuHex) intercalated into the dsDNA grooves to generate the amplified signal...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29309865/nmr-based-structure-reveals-groove-binding-of-mitoxantrone-to-two-sites-of-d-ttagggt-4-having-human-telomeric-dna-sequence-leading-to-thermal-stabilization-of-g-quadruplex
#10
Sweta Tripathi, Ritu Barthwal
Interaction of mitoxantrone (MTX) with G-quadruplex, leading to inhibition of telomerase enzyme and anticancer action, is not understood. Titrations of MTX with [d-(TTAGGGT)]4, comprising human telomere single repeat sequence, have been monitored by fluorescence and 1H/31PNMR spectroscopy. Binding induces chemical shift changes in GNH (~0.3ppm), T2/T7/A3 base protons and sequence specific shifts in 31P resonances. Absence of large downfield shifts in 31P signals and presence of all sequential NOEs show that classical intercalation of drug between base quartets does not occur...
January 5, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29305726/evaluation-of-necrosis-avidity-and-potential-for-rapid-imaging-of-necrotic-myocardium-of-radioiodinated-hypocrellins
#11
Qiaomei Jin, Juanzhi Zhao, Meng Gao, Yuanbo Feng, Wei Liu, Zhiqi Yin, Tiannv Li, Shaoli Song, Yicheng Ni, Jian Zhang, Dejian Huang, Dongjian Zhang
PURPOSE: Rapid noninvasive delineation of necrotic myocardium in ischemic regions is very critical for risk stratification and clinical decision-making but still challenging. This study aimed to evaluate the necrosis avidity of radioiodinated hypocrellins and its potential for rapidly imaging necrotic myocardium. PROCEDURES: The aggregation constants of four natural hypocrellins were analyzed by UV/vis spectroscopy. Then, they were radiolabeled with iodine-131 by iodogen oxidation method...
January 5, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29299569/influence-of-the-manganese-and-cobalt-content-on-the-electrochemical-performance-of-p2-na0-67mnxco1-xo2-cathodes-for-sodium-ion-batteries
#12
K Hemalatha, M Jayakumar, A S Prakash
The resurgence of sodium-ion batteries in recent years is due to their potential ability to form intercalation compounds possessing a high specific capacity and energy density comparable to existing lithium systems. To comprehend the role of cobalt substitution in the structure and electrochemical performance of Na0.67MnO2, the solid solutions of P2-Na0.67MnxCo1-xO2 (x = 0.25, 0.5, 0.75) are synthesized and characterized. The XRD-Rietveld analysis revealed that the Co-substitution in Na0.67MnO2 decreases lattice parameters 'a' and 'c' resulting in the contraction of MO6 octahedra and the enlargement of inter-layer 'd' spacing...
January 4, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29274491/photoactive-platinum-ii-complexes-of-nonsteroidal-anti-inflammatory-drug-naproxen-interaction-with-biological-targets-antioxidant-activity-and-cytotoxicity
#13
Payal Srivastava, Khushbu Singh, Madhu Verma, Sri Sivakumar, Ashis K Patra
The effect on the therapeutic efficacy of Pt(II) complexes on combining non-steroidal anti-inflammatory drugs (NSAIDs) is an attractive strategy to circumvent chronic inflammation mediated by cancer and metastasis. Two square-planar platinum(II) complexes: [Pt(dach)(nap)Cl] (1) and [Pt(dach)(nap)2] (2), where dach = (1R,2R)-dichloro(cyclohexane-1,2-diamine) and NSAID drug naproxen (nap), have been designed for studying their biological activity. The naproxen bound to the Pt(II) centre get released upon photoirradiation with low-power UV-A light as confirmed by the significant enhancement in emission intensities of the complexes...
December 8, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29274339/thermodynamic-and-spectroscopic-investigations-of-tmpyp4-association-with-guanine-and-cytosine-rich-dna-and-rna-repeats-of-c9orf72
#14
Hasan Alniss, Bita Zamiri, Melisa Khalaj, Christopher E Pearson, Robert B Macgregor
BACKGROUND: An expansion of the hexanucleotide repeat (GGGGCC)n·(GGCCCC)n in the C9orf72 promoter has been shown to be the cause of Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The C9orf72 repeat can form four-stranded structures; the cationic porphyrin (TMPyP4) binds and distorts these structures. METHODS: Isothermal titration calorimetry (ITC), and circular dichroism (CD) were used to study the binding of TMPyP4 to the C-rich and G-rich DNA and RNA oligos containing the hexanucleotide repeat at pH 7...
December 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29260852/targeting-the-prion-like-aggregation-of-mutant-p53-to-combat-cancer
#15
Jerson L Silva, Elio A Cino, Iaci N Soares, Vitor F Ferreira, Guilherme A P de Oliveira
Prion-like behavior of several amyloidogenic proteins has been demonstrated in recent years. Despite having functional roles in some cases, irregular aggregation can have devastating consequences. The most commonly known amyloid diseases are Alzheimer's, Parkinson's, and Transmissible Spongiform Encephalopathies (TSEs). The pathophysiology of prion-like diseases involves the structural transformation of wild-type (wt) proteins to transmissible forms that can convert healthy proteins, generating aggregates. The mutant form of tumor suppressor protein, p53, has recently been shown to exhibit prion-like properties...
December 20, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29259757/novel-naphthalimide-aminothiazoles-as-potential-multitargeting-antimicrobial-agents
#16
Ying-Ying Chen, Lavanya Gopala, Rammohan R Yadav Bheemanaboina, Han-Bo Liu, Yu Cheng, Rong-Xia Geng, Cheng-He Zhou
A series of novel naphthalimide aminothiazoles were developed for the first time and evaluated for their antimicrobial activity. Some prepared compounds possessed good inhibitory activity against the tested bacteria and fungi. Noticeably, the piperazine derivative 4d displayed superior antibacterial activity against MRSA and Escherichia coli with MIC values of 4 and 8 μg/mL, respectively, to reference drugs. The most active compound 4d showed low toxicity against mammalian cells with no obvious triggering of the development of bacterial resistance, and it also possessed rapid bactericidal efficacy and efficient membrane permeability...
December 14, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29259297/hyperstretching-dna
#17
Koen Schakenraad, Andreas S Biebricher, Maarten Sebregts, Brian Ten Bensel, Erwin J G Peterman, Gijs J L Wuite, Iddo Heller, Cornelis Storm, Paul van der Schoot
The three-dimensional structure of DNA is highly susceptible to changes by mechanical and biochemical cues in vivo and in vitro. In particular, large increases in base pair spacing compared to regular B-DNA are effected by mechanical (over)stretching and by intercalation of compounds that are widely used in biophysical/chemical assays and drug treatments. We present single-molecule experiments and a three-state statistical mechanical model that provide a quantitative understanding of the interplay between B-DNA, overstretched DNA and intercalated DNA...
December 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/29245062/comparative-studies-on-dna-binding-and-in-vitro-antitumor-activity-of-enantiomeric-ruthenium-ii-complexes
#18
Wen-Xu Hong, Fengwen Huang, Tianwen Huan, Xu Xu, Qingguo Han, Gaofeng Wang, Hong Xu, Shan Duan, Yongheng Duan, Xun Long, Ying Liu, Zhangli Hu
A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)2PBIP]2+ {bpy=2,2'-bipyridine, PBIP=2-(4-bromophenyl)imidazo[4,5-f]1,10-phenanthroline} have been synthesized and characterized. The systematic comparative studies between two enantiomers on their DNA binding-behaviors with calf thymus DNA (CT DNA) were carried out by viscosity measurements, spectrophotometric methods and molecular simulation technology. Additional assays were performed to explore the cytotoxicity of the ruthenium(II) enantiomers against tumor cell lines...
November 29, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29243872/binding-affinity-and-in-vitro-cytotoxicity-of-harmaline-targeting-different-motifs-of-nucleic-acids-an-ultimate-drug-designing-approach
#19
Paromita Bhattacharjee, Tapas Ghosh, Sarita Sarkar, Prateek Pandya, Kakali Bhadra
The work focuses towards interaction of harmaline, with nucleic acids of different motifs by multispectroscopic and calorimetric techniques. Findings of this study suggest that binding constant varied in the order single-stranded (ss) poly(A) > double-stranded calf thymus (CT) DNA > double-stranded poly(G)·poly(C) > clover leaf tRNAPhe . Prominent structural changes of ss poly(A), CT DNA, and poly(G)· poly(C) with concomitant induction of optical activity in the bound achiral alkaloid molecule was observed, while with tRNAPhe , very weak induced circular dichroism perturbation was seen...
December 15, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29243480/reshaping-the-energy-landscape-transforms-the-mechanism-and-binding-kinetics-of-dna-threading-intercalation
#20
Andrew G Clark, M Nabuan Naufer, Fredrik Westerlund, Per Lincoln, Ioulia Rouzina, Thayaparan Paramanathan, Mark C Williams
Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific molecular characteristics of threading intercalators that result in optimal DNA interactions. Using single molecule techniques, we quantify the binding of a small metalorganic ruthenium threading intercalator (Δ,Δ-B), and compare its binding characteristics to a similar molecule with significantly larger threading moieties (Δ,Δ-P)...
December 15, 2017: Biochemistry
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