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https://www.readbyqxmd.com/read/28629072/photodynamic-effect-of-light-harvesting-long-lived-triplet-excited-state-ruthenium-ii-polyimine-coumarin-complexes-dna-binding-photocleavage-and-anticancer-studies
#1
Raju Nomula, Xueyan Wu, Jianzhang Zhao, Nagegownivari R Munirathnam
Two coumarin based Ru(II)-polyimine complexes (Ru-1 and Ru-2) showing intense absorption of visible light and long-lived triplet excited states (~12-15μs) were used for study of the interaction with DNA. The binding of the complexes with CT-DNA were studied by UV-vis, fluorescence and time-resolved nanosecond transient absorption (ns-TA) spectroscopy. The results suggesting that the complexes interact with CT-DNA by intercalation mode of binding, showing the binding constants (Kb) 6.47×10(4) for Ru-1 and 5...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628723/new-multifunctional-radiotheranostic-agent-for-dual-targeting-of-breast-cancer-cells
#2
Filipe Vultos, Célia Fernandes, Filipa Mendes, Fernanda Marques, João Correia, Isabel Santos, Lurdes Gano
A straightforward synthetic route for a new multifunctional DOTA derivative is described. To prove the versatility of this prochelator to prepare radiolabelled hybrid compounds containing two different biological targeting moieties, an antitumoral agent (e.g. DNA intercalating agent) and an Estrogen Receptor ligand (e.g. LXXLL based peptide) were regiospecifically conjugated to the DOTA derivative. The bifunctional probe was radiolabelled with the Auger electron emitter Indium-111 and the radioconjugate demonstrated to induce DNA damage in vitro which along with the nuclear internalization exhibited in breast cancer cells might enhance its therapeutic activity...
June 19, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28621352/effects-of-oxygenation-on-the-intercalation-of-1-10-phenanthroline-5-6-4-7-dione-between-dna-base-pairs-a-computational-study
#3
Aurellia Galliot, Adrià Gil, Maria José Calhorda
The effects of oxygen in positions 4,7 and 5,6 of phenanthroline when this ligand intercalates between guanine-cytosine and adenine-thymine DNA base pairs (GC/CG and AT/TA) have been studied at the M06-2X/6-31+G(d,p) level of calculation. We focused on the changes in the structure, stabilization and energy contributions in the analysis of the interaction. The obtained trends in stabilization are explained by a model including repulsive Pauli (ΔEPauli) contributions, and attractive dispersion (ΔEdisp), orbital (ΔEorb) and electrostatic (ΔEelstat) contributions to energy...
June 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28611596/ethidium-bromide-modifies-the-agarose-electrophoretic-mobility-of-cag%C3%A2-ctg-alternative-dna-structures-generated-by-pcr
#4
Mário Gomes-Pereira, Darren G Monckton
The abnormal expansion of unstable simple sequence DNA repeats can cause human disease through a variety of mechanisms, including gene loss-of-function, toxic gain-of-function of the encoded protein and toxicity of the repeat-containing RNA transcript. Disease-associated unstable DNA repeats display unusual biophysical properties, including the ability to adopt non-B-DNA structures. CAG•CTG trinucleotide sequences, in particular, have been most extensively studied and they can fold into slipped-stranded DNA structures, which have been proposed as mutation intermediates in repeat size expansion...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28595065/new-quinoline-arylamidine-hybrids-synthesis-dna-rna-binding-and-antitumor-activity
#5
Luka Krstulović, Ivana Stolić, Marijana Jukić, Teuta Opačak-Bernardi, Kristina Starčević, Miroslav Bajić, Ljubica Glavaš-Obrovac
Four series of new hybrid molecules with 7-chloroquinoline and arylamidine moieties joined through the rigid -O- (groups I (2a-g) and II (5a-g)) or flexible -NH-CH2-CH2-O- (groups III (8a-g) and IV (10a-g)) linker were synthesized, and their DNA/RNA binding properties and cytotoxic activity were tested, against several human cancer lines. The compounds and their interaction with DNA and RNA were studied by UV-Vis and CD spectroscopy. The obtained results showed that the binding affinity of the investigated compounds increases proportionally with the increase of the length and number of groups able to form hydrogen bonds with ds-polynucleotides...
May 28, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28593371/design-of-a-novel-electrochemical-biosensor-based-on-intramolecular-g-quadruplex-dna-for-selective-determination-of-lead-ii-ions
#6
Maryam Ebrahimi, Jahan Bakhsh Raoof, Reza Ojani
An electrochemical DNA biosensor based on a G-quadruplex (G4) for the sensitive determination of Pb(2+) was reported using a carbon paste electrode (CPE) or a multi-walled carbon nanotube paste electrode (MWCNTPE) as working electrodes, ethyl green (EG) as a new G4 intercalator, and a single-stranded nucleic acid sequence rich in guanine (G) as DNA probe. Electrochemical determination of Pb(2+) relied on probe structural changes from single - stranded to the stabilized intramolecular G4 in the presence of Pb(2+), which caused a change in the current of the EG reduction peak due to the intercalation of EG into the G4 structure...
June 7, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28590495/structure-based-design-of-novel-combinatorially-generated-nbtis-as-potential-dna-gyrase-inhibitors-against-various-staphylococcus-aureus-mutant-strains
#7
Anja Kolaric, Nikola Minovski
Although intercalating agents such as quinolones have had proven therapeutic success as antibacterial agents for more than 40 years, new forms of quinolone-based resistance in bacteria are continually emerging. To alleviate this problem, a new class of antibacterials is urgently needed; recently, novel bacterial topoisomerase inhibitors (NBTIs) have been found to be particularly important. Based on 67 experimentally evaluated NBTIs against wild-type (WT) DNA gyrase originating from Staphylococcus aureus, a predictive QSAR model was initially constructed and validated and was later used for in silico prediction of biological activities for an in house designed compound library of 548 novel drug-like NBTI combinatorial analogs...
June 7, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28590119/parallel-evaluation-of-melting-temperatures-of-dnas-in-the-arrayed-droplets-through-the-fluorescence-from-dna-intercalators
#8
Yuzuru Shimazaki, Junko Tanaka, Yoshinobu Kohara, Masao Kamahori, Takeshi Sakamoto
Parallel evaluation of melting temperatures (Tm's) of DNA molecules in multiple floating droplets (20 μm in diameter) was demonstrated. The Tm values were evaluated from the melting curves which were observed through the fluorescence from the DNA intercalators. The Tm values measured in the droplets corresponded well to those measured in the bulk, indicating the validity of the measurement. The parallel evaluation of Tm's was realized by observing melting curves of DNAs in the different droplets at the same time using the "droplet guide", which guided and fixed the floating droplets to the designated points in the observing plane...
June 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28585811/dinuclear-ru-ii-bipy-2-derivatives-structural-biological-and-in-vivo-zebrafish-toxicity-evaluation
#9
Oscar A Lenis-Rojas, Catarina Roma-Rodrigues, Alexandra R Fernandes, Fernanda Marques, David Pérez-Fernández, Jorge Guerra-Varela, Laura Sánchez, Digna Vázquez-García, Margarita López-Torres, Alberto Fernández, Jesús J Fernández
Ruthenium-based drugs exhibit interesting properties as potential anticancer pharmaceuticals. We herein present the synthesis and characterization of a new family of ruthenium complexes with formulas [{Ru(bipy)2}2(μ-L)][CF3SO3]4 (L = bptz, 1a) and [{Ru(bipy)2}2(μ-L)][CF3SO3]2 (L = arphos, 2a; dppb, 3a; dppf, 4a), which were synthesized from the Ru(II) precursor compound cis-Ru(bipy)2Cl2. The complexes were characterized by elemental analysis, mass spectrometry, (1)H and (31)P{(1)H} NMR, IR spectroscopy, and conductivity measurements...
June 6, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28585379/groel-is-a-suitable-genetic-marker-for-detecting-vibrio-parahaemolyticus-by-loop-mediated-isothermal-amplification-lamp-assay
#10
Mahbubul Pratik Siddique, Won Je Jang, Jong Min Lee, Sun-Hee Ahn, Sharmin Suraiya, Chang-Hoon Kim, In-Soo Kong
A groEL gene-based loop-mediated isothermal amplification (LAMP) assay was developed to detect Vibrio parahaemolyticus in contaminated seafood and water. The assay was optimized and conducted at 63°C for 40 min using Bacillus stearothermophilus (Bst) DNA polymerase, large fragment. Amplification was analyzed via multiple detection methods, including opacity, formation of white precipitate, DNA intercalating dyes (ethidium bromide and SYBR Green I), metal ion-binding indicator dye, calcein, and 2% agarose gel electrophoresis...
June 5, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28584239/the-effect-of-the-neutral-cytidine-protonated-analogue-pseudoisocytidine-on-the-stability-of-i-motif-structures
#11
B Mir, X Solés, C González, N Escaja
Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C(+) stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C(+) or psC:psC(+) hemiprotonated base pairs are formed instead of their neutral analogs...
June 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28581004/dna-origami-gold-nanorod-hybrid-nanostructures-for-the-circumvention-of-drug-resistance
#12
Linlin Song, Qiao Jiang, Jianbing Liu, Na Li, Qing Liu, Luru Dai, Yuan Gao, Weili Liu, Dongsheng Liu, Baoquan Ding
We herein demonstrate that DNA origami can work as a multifunctional platform integrating a chemotherapeutic drug (doxorubicin), gold nanorods and a tumour-specific aptamer MUC-1, to realize the effective circumvention of drug resistance. Doxorubicin (DOX) was loaded efficiently onto DNA origami through base pair intercalation and surface-modified gold nanorods (AuNRs) were assembled onto the DNA origami through DNA hybridization. Due to the active targeting effect of the assembled aptamers, the multifunctional nanostructures achieved increased cellular internalization of DOX and AuNRs...
June 5, 2017: Nanoscale
https://www.readbyqxmd.com/read/28578331/whole-genome-sequence-identified-a-rare-homozygous-pathogenic-mutation-of-the-dsg2-gene-in-a-familial-arrhythmogenic-cardiomyopathy-involving-both-ventricles
#13
Yubi Lin, Qianhuan Zhang, Zhi An Zhong, Zhe Xu, Siqi He, Fang Rao, Yang Liu, Jiaojiao Tang, Feng Wang, Hui Liu, Jiajun Xie, Hongmei Wu, Shuxia Wang, Xin Li, Zhixin Shan, Chunyu Deng, Zili Liao, Hai Deng, Hongtao Liao, Yumei Xue, Wanqun Chen, Xianzhang Zhan, Bin Zhang, Shulin Wu
BACKGROUND: This study was designed to identify the pathogenic mutation in a Chinese family with arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) using whole genome sequencing (WGS). METHODS AND RESULTS: Probands II:1 and II:2 underwent routine examinations for diagnosis. Genomic DNA was extracted from the peripheral blood of family members and analyzed using WGS. A total of 60,285 single-nucleotide polymorphisms (SNP) and 13,918 insertions/deletions (InDel) occurring in the exonic regions of genes and predisposing to cardiomyopathies and arrhythmias were identified...
June 3, 2017: Cardiology
https://www.readbyqxmd.com/read/28577500/a-genosensor-for-detection-of-consensus-dna-sequence-of-dengue-virus-using-zno-pt-pd-nanocomposites
#14
Chaitali Singhal, C S Pundir, Jagriti Narang
An electrochemical genosensor based on Zinc oxide/platinum-palladium (ZnO/Pt-Pd) modified fluorine doped tin oxide (FTO) glass plate was fabricated for detection of consensus DNA sequence of Dengue virus (DENV) using methylene blue (MB) as an intercalating agent. To achieve it, probe DNA (PDNA) was immobilized on the surface of ZnO/Pt-Pd nanocomposites modified FTO electrode. The synthesized nano-composites were characterized by high resolution transmission electron microscopy (HRTEM), energy dispersive X-ray analysis (EDX), atomic force microscopy (AFM), scanning electron microscopy (SEM), UV-Vis spectroscopy, X-ray diffraction (XRD) analysis and Fourier transform infra-red (FTIR) spectroscopy...
May 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28575934/dna-interaction-antioxidant-and-in-vitro-cytotoxic-activities-of-some-mononuclear-metal-ii-complexes-of-a-bishydrazone-ligand
#15
Chellaian Justin Dhanaraj, Jijo Johnson
A tridentate ONO donor bishydrazone ligand, 3-(((3-oxoindolin-2-ylidene)hydrazono) methyl)quinoxalin-2(1H)-one (L) was synthesized. Using the ligand, mononuclear metal complexes of the type [M(L)(OAc)] (M=Co(II), Ni(II), Cu(II) and Zn(II), OAc=-OCOCH3) were synthesized. The newly synthesized compounds were thoroughly characterized by various analytical and spectral techniques. Tetrahedral geometry was proposed for Co(II), Ni(II) and Zn(II) complexes, while square planar geometry has been assigned to the Cu(II) complex...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28572901/allosteric-dna-nanoswitches-for-controlled-release-of-a-molecular-cargo-triggered-by-biological-inputs
#16
Marianna Rossetti, Simona Ranallo, Andrea Idili, Giuseppe Palleschi, Alessandro Porchetta, Francesco Ricci
Here we demonstrate the rational design of a new class of DNA-based nanoswitches which are allosterically regulated by specific biological targets, antibodies and transcription factors, and are able to load and release a molecular cargo (i.e. doxorubicin) in a controlled fashion. In our first model system we rationally designed a stem-loop DNA-nanoswitch that adopts two mutually exclusive conformations: a "Load" conformation containing a doxorubicin-intercalating domain and a "Release" conformation containing a duplex portion recognized by a specific transcription-factor (here Tata Binding Protein)...
February 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28567680/determination-of-a-new-index-of-sexual-maturity-ism-in-zebra-mussel-using-flow-cytometry-interest-in-ecotoxicology
#17
Gabrielle Magniez, Alban Franco, Alain Geffard, Damien Rioult, Isabelle Bonnard, Laurence Delahaut, Sandrine Joachim, Gaëlle Daniele, Emmanuelle Vulliet, Jean-Marc Porcher, Marc Bonnard
The global dynamic spread of chemical contamination through the aquatic environment calls for the development of biomarkers of interest. Reproduction is a key element to be considered because it is related to the sustainability of species. Spermatogenesis is a complex process that leads to the formation of mature germ cells, whose steps and impairments need to be finely described in ecotoxicological analyses. The physiological process has been commonly described by histological analyses of gonads in different taxa...
May 31, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28567459/identification-of-new-dna-i-motif-binding-ligands-through-a-fluorescent-intercalator-displacement-assay
#18
Qiran Sheng, Joseph C Neaverson, Tasnim Mahmoud, Clare E M Stevenson, Susan E Matthews, Zoë A E Waller
i-Motifs are quadruplex DNA structures formed from sequences rich in cytosine and held together by intercalated, hemi-protonated cytosine-cytosine base pairs. These sequences are prevalent in gene promoter regions and may play a role in gene transcription. Targeting these structures with ligands could provide a novel way to target genetic disease but there are very few ligands which have been shown to interact with i-motif DNA. Fluorescent intercalator displacement (FID) assays are a simple way to screen ligands against DNA secondary structures...
June 1, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28566685/monitoring-zeo-apoptotic-potential-in-2d-and-3d-cell-cultures-and-associated-spectroscopic-evidence-on-mode-of-interaction-with-dna
#19
Fahimeh Salehi, Hossein Behboudi, Gholamreza Kavoosi, Sussan K Ardestani
Recognizing new anticancer compounds to improve Breast cancer treatment seems crucial. Essential oil of Zataria Multiflora (ZEO) is a secondary metabolite with some biological properties, yet underlying cellular and molecular anticancer properties of ZEO is unclear. GC/MS analysis revealed that carvacrol is the major ingredient of the essential oil. ZEO increasingly suppressed viability in MDA-MB-231, MCF-7 and T47D Breast cancer cells while nontoxic to L929 normal cells in monolayer cell cultures (2D), whereas MDA-MB-231 multicellular spheroids (3D) were more resistant to inhibition...
May 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28566001/exploring-encapsulation-mechanism-of-dna-and-mononucleotides-in-sol-gel-derived-silica
#20
Derya Kapusuz, Caner Durucan
The encapsulation mechanism of DNA in sol-gel derived silica has been explored in order to elucidate the effect of DNA conformation on encapsulation and to identify the nature of chemical/physical interaction of DNA with silica during and after sol-gel transition. In this respect, double stranded DNA and dAMP (2'-deoxyadenosine 5'-monophosphate) were encapsulated in silica using an alkoxide-based sol-gel route. Biomolecule-encapsulating gels have been characterized using UV-Vis, (29)Si NMR, FTIR spectroscopy and gas adsorption (BET) to investigate chemical interactions of biomolecules with the porous silica network and to examine the extent of sol-gel reactions upon encapsulation...
January 1, 2017: Journal of Biomaterials Applications
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