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https://www.readbyqxmd.com/read/28821189/y-chromosome-markers-for-the-red-fox
#1
Halie M Rando, Jeremy T Stutchman, Estelle R Bastounes, Jennifer L Johnson, Carlos A Driscoll, Christina S Barr, Lyudmila N Trut, Benjamin N Sacks, Anna V Kukekova
The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However, a megabase of red fox Y-chromosome sequence was recently identified over 2 scaffolds in the reference genome. Here, these scaffolds were scanned for repeated motifs, revealing 194 likely microsatellites. Twenty-three of these loci were selected for primer development and, after testing, produced a panel of 11 novel markers that were analyzed alongside 2 markers previously developed for the red fox from dog Y-chromosome sequence...
September 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28821188/panmixia-and-limited-interspecific-introgression-in-coyotes-canis-latrans-from-west-virginia-and-virginia-usa
#2
Justin H Bohling, Lauren L Mastro, Jennifer R Adams, Eric M Gese, Sheldon F Owen, Lisette P Waits
The expansion of coyotes (Canis latrans) into the eastern United States has had major consequences for ecological communities and wildlife managers. Despite this, there has been little investigation of the genetics of coyotes across much of this region, especially outside of the northeast. Understanding patterns of genetic structure and interspecific introgression would provide insights into the colonization history of the species, its response to the modern environment, and interactions with other canids. We examined the genetic characteristics of 121 coyotes from the mid-Atlantic states of West Virginia and Virginia by genotyping 17 polymorphic nuclear DNA microsatellite loci...
September 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28821184/repetitive-dna-in-the-catfish-genome-rdna-microsatellites-and-tc1-mariner-transposon-sequences-in-imparfinis-species-siluriformes-heptapteridae
#3
Juceli Gonzalez Gouveia, Ivan Rodrigo Wolf, Laurival Antonio Vilas-Boas, John Seymour Heslop-Harrison, Trude Schwarzacher, Ana Lúcia Dias
Physical mapping of repetitive DNA families in the karyotypes of fish is important to understand the organization and evolution of different orders, families, genera, or species. Fish in the genus Imparfinis show diverse karyotypes with various diploid numbers and ribosomal DNA (rDNA) locations. Here we isolated and characterized Tc1-mariner nucleotide sequences from Imparfinis schubarti, and mapped their locations together with 18S rDNA, 5S rDNA, and microsatellite probes in Imparfinis borodini and I. schubarti chromosomes...
September 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28821076/confirmation-and-measurement-of-hydrophobic-interaction-in-sol-gel-system-of-konjac-glucomannan-with-different-degree-of-deacetylation
#4
Chen Xin, Jian Chen, Hongshan Liang, Jiawei Wan, Jing Li, Bin Li
Fluorescence measurement was used as a tool to study the hydrophobic interaction in sol-gel system of konjac glucomannan (KGM) with different degree of deacetylation (DD). In the concentration from 0 to 10g/L, I1/I3 ratio of native KGM declined from 1.75 to 1.25. Before and after heating, the critical associating concentration of the native KGM was 0.60g/L and 0.95g/L respectively, but this value of deacetylated KGM (DD=32.58%) was 0.58g/L and 0.45g/L respectively. The addition of Na2SO4 and NaSCN exhibited more significant influence on deacetylated KGM compared with native KGM...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820970/utility-of-the-comprehensive-marijuana-motives-questionnaire-among-medical-cannabis-patients
#5
Kipling M Bohnert, Erin E Bonar, J Todd Arnedt, Deirdre A Conroy, Maureen A Walton, Mark A Ilgen
BACKGROUND: Little is known about motives for cannabis use among the population of adults using cannabis medically. Therefore, we evaluated the performance of the 12 factor, 36-item Comprehensive Marijuana Motives Questionnaire (CMMQ) among a sample of medical cannabis patients. METHODS: Study participants were adults ages 21years or older with scheduled appointments to obtain new or renewed medical cannabis certification from clinics in one Midwestern state (n=1116)...
August 3, 2017: Addictive Behaviors
https://www.readbyqxmd.com/read/28820969/a-disulfide-stabilized-human-vl-single-domain-antibody-library-is-a-source-of-soluble-and-highly-thermostable-binders
#6
Kevin A Henry, Hiba Kandalaft, Michael J Lowden, Martin A Rossotti, Henk van Faassen, Greg Hussack, Yves Durocher, Dae Young Kim, Jamshid Tanha
We have previously shown that incorporation of a second intradomain disulfide linkage into camelid VHH and human VH/VL single-domain antibodies confers increased thermostability. Here, we explored the effects of introducing an additional disulfide linkage, formed between Cys48 and Cys64 (Kabat numbering), into a phage-displayed synthetic human VL library. In comparison to an identical library bearing only the highly conserved Cys23-Cys88 disulfide linkage, the disulfide-stabilized VL library tolerated a similar degree of randomization but retained a higher level of functional diversity after selection with protein L...
August 15, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28820946/proteogenomic-investigation-of-strain-variation-in-clinical-mycobacterium-tuberculosis-isolates
#7
Tiaan Heunis, Anzaan Dippenaar, Robin M Warren, Paul D van Helden, Ruben G van der Merwe, Nicolaas C Gey van Pittius, Arnab Pain, Samantha L Sampson, David L Tabb
Mycobacterium tuberculosis consists of a large number of different strains that display unique virulence characteristics. Whole-genome sequencing has revealed substantial genetic diversity among clinical M. tuberculosis isolates, and elucidating the phenotypic variation encoded by this genetic diversity will be of utmost importance to fully understand M. tuberculosis biology and pathogenicity. In this study we integrated whole-genome sequencing and mass spectrometry (GeLC-MS/MS) to reveal strain-specific characteristics in the proteomes of two clinical M...
August 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28820903/prominent-facilitation-at-beta-and-gamma-frequency-range-revealed-with-physiological-calcium-concentration-in-adult-mouse-piriform-cortex-in-vitro
#8
Marie Gleizes, Simon P Perrier, Caroline Fonta, Lionel G Nowak
Neuronal activity is characterized by a diversity of oscillatory phenomena that are associated with multiple behavioral and cognitive processes, yet the functional consequences of these oscillations are not fully understood. Our aim was to determine whether and how these different oscillatory activities affect short-term synaptic plasticity (STP), using the olfactory system as a model. In response to odorant stimuli, the olfactory bulb displays a slow breathing rhythm as well as beta and gamma oscillations...
2017: PloS One
https://www.readbyqxmd.com/read/28820880/knock-out-of-a-mitochondrial-sirtuin-protects-neurons-from-degeneration-in-caenorhabditis-elegans
#9
Rachele Sangaletti, Massimo D'Amico, Jeff Grant, David Della-Morte, Laura Bianchi
Sirtuins are NAD⁺-dependent deacetylases, lipoamidases, and ADP-ribosyltransferases that link cellular metabolism to multiple intracellular pathways that influence processes as diverse as cell survival, longevity, and cancer growth. Sirtuins influence the extent of neuronal death in stroke. However, different sirtuins appear to have opposite roles in neuronal protection. In Caenorhabditis elegans, we found that knock-out of mitochondrial sirtuin sir-2.3, homologous to mammalian SIRT4, is protective in both chemical ischemia and hyperactive channel induced necrosis...
August 18, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28820870/controversies-in-the-identification-and-management-of-acute-pulmonary-hypertension-in-preterm-neonates
#10
REVIEW
Regan E Giesinger, Kiran More, Jodie Odame, Amish Jain, Robert P Jankov, Patrick J McNamara
It is increasingly recognized that the abnormal physiologic consequences of pulmonary hypertension (PH) may contribute to poor cardiopulmonary health in premature babies. Conflicting literature has led to clinical uncertainty, pathological misinterpretation and variability in treatment approaches among practitioners. There are several disorders with overlapping and interrelated presentations and other disorders with a similar clinical phenotype but diverse pathophysiological contributors. In this review, we provide a diagnostic approach for acute HRF in the preterm neonate, outline the pathophysiological conditions that may present as acute PH and discuss the implications of high pulmonary vascular resistance (PVR) on the cardiovascular system...
August 18, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28820749/surgical-pathology-of-gastrointestinal-stromal-tumors-practical-implications-of-morphologic-and-molecular-heterogeneity-for-precision-medicine
#11
Gregory W Charville, Teri A Longacre
Gastrointestinal stromal tumor (GIST), the most common mesenchymal neoplasm of the gastrointestinal tract, exhibits diverse histologic and clinical manifestations. With its putative origin in the gastrointestinal pacemaker cell of Cajal, GIST can arise in association with any portion of the tubular gastrointestinal tract. Morphologically, GISTs are classified as spindled or epithelioid, though each of these subtypes encompasses a broad spectrum of microscopic appearances, many of which mimic other histologic entities...
August 17, 2017: Advances in Anatomic Pathology
https://www.readbyqxmd.com/read/28820708/clinical-spectrum-etiology-and-outcome-of-neurological-disorders-in-the-rural-hospital-of-mosango-the-democratic-republic-of-congo
#12
Deby Mukendi, Jean-Roger Lilo Kalo, Alain Mpanya, Luigi Minikulu, Tharcisse Kayembe, Pascal Lutumba, Barbara Barbé, Philippe Gillet, Jan Jacobs, Harry Van Loen, Cedric P Yansouni, François Chappuis, Raffaella Ravinetto, Kristien Verdonck, Marleen Boelaert, Andrea S Winkler, Emmanuel Bottieau
There is little published information on the epidemiology of neurological disorders in rural Central Africa, although the burden is considered to be substantial. This study aimed to investigate the pattern, etiology, and outcome of neurological disorders in children > 5 years and adults admitted to the rural hospital of Mosango, province of Kwilu, Democratic Republic of Congo, with a focus on severe and treatable infections of the central nervous system (CNS). From September 2012 to January 2015, 351 consecutive patients hospitalized for recent and/or ongoing neurological disorder were prospectively evaluated by a neurologist, subjected to a set of reference diagnostic tests in blood or cerebrospinal fluid, and followed-up for 3-6 months after discharge...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820667/helping-young-people-stay-afloat-a-qualitative-study-of-community-resources-and-supports-for-lgbtq-adolescents-in-the-us-and-canada
#13
Marla E Eisenberg, Christopher J Mehus, Elizabeth M Saewyc, Heather L Corliss, Amy L Gower, Richard Sullivan, Carolyn M Porta
LGBTQ youth are at increased risk of poor health outcomes. This qualitative study gathered data from LGBTQ adolescents regarding their communities and describes the resources they draw upon for support. We conducted 66 go-along interviews with diverse LGBTQ adolescents (mean age = 16.6) in Minnesota, Massachusetts and British Columbia in 2014-2015, in which interviewers accompanied participants in their communities to better understand those contexts. Their responses were systematically organized and coded for common themes, reflecting levels of the social ecological model...
August 18, 2017: Journal of Homosexuality
https://www.readbyqxmd.com/read/28820622/latent-growth-curve-modeling-of-non-injection-drug-use-and-condomless-sexual-behavior-from-ages-18-to-21-in-gay-bisexual-and-other-ymsm-the-p18-cohort-study
#14
Perry N Halkitis, Kristen Bub, Christopher B Stults, Francesca C Bates, Farzana Kapadia
BACKGROUND: HIV/AIDS continues to be a health disparity faced by sexual minority men, and is exacerbated by non-injection drug use. OBJECTIVES: We sought to delineate growth in non-injection drug use and condomless sex in a sample of racially and economically diverse of gay, bisexual, and other young men who have sex with men (YMSM) as they emerged into adulthood between the ages of 18 and 21 and who came of age in the post-HAART era. METHODS: Behavioral data on drug use and condomless sex, collected via a calendar based technique over 7 waves of a cohort study of 600 YMSM, were analyzed using latent growth curve modeling to document patterns of growth in these behaviors, their associations, and the extent to which patterns and associations are moderated by race/ethnicity and socioeconomic status...
August 18, 2017: Substance Use & Misuse
https://www.readbyqxmd.com/read/28820608/skeletal-muscle-ultrasound-in-critical-care-a-tool-in-need-of-translation
#15
Marina Mourtzakis, Selina Parry, Bronwen Connolly, Zudin Puthucheary
With the emerging interest in documenting and understanding muscle atrophy and function in critically ill patients and survivors, ultrasonography has transformational potential in measuring muscle quantity and quality. Here, we discuss the importance of quantifying skeletal muscle in the ICU setting. We also identify the merits and limitations of various modalities that are capable of accurately and precisely measuring muscularity. Ultrasound is emerging as a potentially powerful tool for skeletal muscle quantification; however, there are key challenges that need to be addressed in future work to ensure useful interpretation and comparability of results across diverse observational and interventional studies...
August 18, 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/28820590/conformational-heterogeneity-in-a-fully-complementary-dna-three-way-junction-with-a-gc-rich-branchpoint
#16
Anita Toulmin, Laura E Baltierra-Jasso, Michael J Morten, Tara Sabir, Peter McGlynn, Gunnar F Schröder, Brian O Smith, Steven W Magennis
DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully-complementary 3WJ and found that it contained unpaired bases at the branchpoint, in support of previous observations of branch flexibility and branchpoint reactivity. By combining high-resolution single-molecule FRET, molecular modeling, time-resolved ensemble fluorescence spectroscopy and the first 19F NMR observations of fully complementary 3WJs, we now show that the 3WJ structure can adopt multiple distinct conformations depending upon the sequence at the branchpoint...
August 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28820585/chemo-enzymatic-assembly-of-isotopically-labeled-folates
#17
Antonio Angelastro, William Michael Dawson, Louis Y P Luk, E Joel Loveridge, Rudolf K Allemann
Pterin-containing natural products have diverse functions in life but an efficient and easy scheme for their in vitro synthesis is not available. Here, we report a chemo-enzymatic 14-step, one-pot synthesis that can be used to generate 13C- and 15N-labeled dihydrofolates (H2F) from glucose, guanine and p-aminobenzoyl-L-glutamic acid. This synthesis stands out from previous approaches to produce H2F in that the average yield of each step is >91% and it requires only one single purification step. The use of a one-pot reaction allowed us to overcome potential problems with individual steps during the synthesis...
August 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28820533/actinomycetes-still-a-source-of-novel-antibiotics
#18
REVIEW
Olga Genilloud
Covering: 2006 to 2017Actinomycetes have been, for decades, one of the most important sources for the discovery of new antibiotics with an important number of drugs and analogs successfully introduced in the market and still used today in clinical practice. The intensive antibacterial discovery effort that generated the large number of highly potent broad-spectrum antibiotics, has seen a dramatic decline in the large pharma industry in the last two decades resulting in a lack of new classes of antibiotics with novel mechanisms of action reaching the clinic...
August 18, 2017: Natural Product Reports
https://www.readbyqxmd.com/read/28820508/complementary-medicine-and-the-role-of-oncology-nurses-in-an-acute-care-hospital-the-gap-between-attitudes-and-practice
#19
Hanna Admi, Yael Eilon-Moshe, Eran Ben-Arye
PURPOSE/OBJECTIVES: To describe hospital nurses' knowledge, attitudes, and practices regarding complementary medicine (CM); to compare the knowledge and attitudes of nurse managers to staff nurses with diverse oncology experience; and to assess attitudes toward integrating CM into the role of the hospital oncology nurse. 
. DESIGN: Descriptive, cross-sectional study.
. SETTING: Rambam Health Care Campus in northern Israel.
. SAMPLE: A convenience sample of 434 hospital nurses with varied oncology experience...
September 1, 2017: Oncology Nursing Forum
https://www.readbyqxmd.com/read/28820456/deciphering-single-nucleotide-polymorphisms-and-evolutionary-trends-in-isolates-of-the-cydia-pomonella-granulovirus
#20
Jörg T Wennmann, Pit Radtke, Karolin E Eberle, Gianpiero Gueli Alletti, Johannes A Jehle
Six complete genome sequences of Cydia pomonella granulovirus (CpGV) isolates from Mexico (CpGV-M and CpGV-M1), England (CpGV-E2), Iran (CpGV-I07 and CpGV-I12), and Canada (CpGV-S) were aligned and analyzed for genetic diversity and evolutionary processes. The selected CpGV isolates represented recently identified phylogenetic lineages of CpGV, namely, the genome groups A to E. The genomes ranged from 120,816 bp to 124,269 bp. Several common differences between CpGV-M, -E2, -I07, -I12 and -S to CpGV-M1, the first sequenced and published CpGV isolate, were highlighted...
August 18, 2017: Viruses
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