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Byungseop Yang, Jong Chul Kim, Jihyoun Seong, Giyoong Tae, Inchan Kwon
Human serum albumin (HSA) has been investigated as a serum half-life extender of therapeutic proteins thanks to its unusually long serum half-life. However, in mice, the serum half-life of a HSA-conjugated protein was much shorter than that of HSA in humans, likely due to the species-dependent nature of albumin-FcRn interactions. Herein, we investigated species-dependent albumin-FcRn interactions using species-matched albumin (mouse serum albumin) and species-mismatched albumin (HSA) in non-transgenic mice...
June 8, 2018: Biomaterials Science
Pankaj S Mahajan, Vivek T Humne, Subhash D Tanpure, Santosh B Mhaske
The Beckmann rearrangement of ketoximes, mediated by ammonium persulfate-dimethyl sulfoxide as a reagent, has been achieved under neutral conditions. Based on the radical trapping and (18)O-labeling experiments, the transformation follows a mechanism involving a radical pathway. The scope and generality of the developed protocol has been demonstrated by 19 examples. The developed protocol and Pd-catalyzed intramolecular double C-H activation were used as key steps in the formal total synthesis of antimalarial natural product isocryptolepine...
July 15, 2016: Organic Letters
S Hajiahmadi, M Panjehpour, M Aghaei, S Mousavi
Adenosine receptors (A1, A2a, A2b and A3) have several physiological and pathological roles in cancer cell lines. The present study was carried out to evaluate the mRNA and protein expression profile and functional role of adenosine receptors in OVCAR-3, Caov-4 and SKOV-3 ovarian cancer cell lines. The levels of mRNA and protein expression of A1, A2a, A2b and A3 adenosine receptors in the ovarian cancer cell lines were measured by Real-time PCR and western blotting. The functional roles of adenosine receptors were investigated through measurement of cAMP levels after agonist treatment...
January 2015: Research in Pharmaceutical Sciences
Mahavir S Naykode, Vivek T Humne, Pradeep D Lokhande
An efficient regioselective iodination of the Fischer-Borsche ring has been achieved using molecular iodine, in a one-pot synthesis. The acid-, metal-, and oxidant-free conditions of the present method are highly convenient and practical. Furthermore, the one-pot direct iodination process is extended to the concise synthesis of glycozoline, 3-formyl-6-methoxy-carbazole, and 6-methoxy-carbazole-3-methylcarboxylate natural alkaloids. This method has been proven to be tolerant to a broad range of functional groups, with good to excellent yields...
February 20, 2015: Journal of Organic Chemistry
Vivek Humne, Yuvraj Dangat, Kumar Vanka, Pradeep Lokhande
A new protocol for the aromatization of tetrahydrocarbazoles has been achieved using a catalytic amount of iodine, giving high yields. The role of iodine in the aromatization has been explained by DFT, and its wide scope is extended to the total synthesis of glycozoline and murrayafoline A. This method has proven to be tolerant of a broad range of functional groups.
July 21, 2014: Organic & Biomolecular Chemistry
M A Khan, S Manna, N Malhotra, J Sengupta, D Ghosh
BACKGROUND & OBJECTIVES: During 6 to 8 wk of gestation, human placental villi show a complex pattern of morphogenesis. There is however, no large scale gene expression study exploring the temporal pattern of the developmental molecular networks in placental villi during the early weeks of gestation. We evaluated the transcriptome profiling of humn placental villus samples obtained from fertile women with voluntarily terminated normal pregnancies between 6-8 wk of gestation. METHODS: Transcriptomic profiles of individual human placental villous samples from 25 women with normal pregnancies during 6 to 8 wk of gestation were examined using human whole genome expression arrays...
January 2014: Indian Journal of Medical Research
Micheline Kirsch-Volders, Stefano Bonassi, Siegfried Knasmueller, Nina Holland, Claudia Bolognesi, Michael F Fenech
The lymphocyte cytokinesis-block micronucleus (CBMN) assay has been applied in hundreds of in vivo biomonitoring studies of humans exposed to genotoxic chemicals because it allows the measurement of both structural and numerical chromosome aberrations. The CBMN cytome assay version which, apart from measuring micronuclei (MN) already present in cells in vivo or expressed ex vivo, also includes measurement of nucleoplasmic bridges (NPB), nuclear buds (NBUD), necrosis and apoptosis, is also increasingly being used in such studies...
January 2014: Mutation Research. Reviews in Mutation Research
Michael Fenech, Micheline Kirsch-Volders, Andrea Rossnerova, Radim Sram, Horst Romm, Claudia Bolognesi, Adarsh Ramakumar, Francoise Soussaline, Christian Schunck, Azeddine Elhajouji, Wagida Anwar, Stefano Bonassi
The use of micronucleus (MN) assays in in vitro genetic toxicology testing, radiation biodosimetry and population biomonitoring to study the genotoxic impacts of environment gene-interactions has steadily increased over the past two decades. As a consequence there has been a strong interest in developing automated systems to score micronuclei, a biomarker of chromosome breakage or loss, in mammalian and human cells. This paper summarises the outcomes of a workshop on this topic, organised by the HUMN project, at the 6th International Conference on Environmental Mutagenesis in Human Populations at Doha, Qatar, 2012...
August 2013: International Journal of Hygiene and Environmental Health
Stefano Bonassi, Erdem Coskun, Marcello Ceppi, Cecilia Lando, Claudia Bolognesi, Sema Burgaz, Nina Holland, Micheline Kirsh-Volders, Siegfried Knasmueller, Errol Zeiger, Deyanira Carnesoltas, Delia Cavallo, Juliana da Silva, Vanessa M de Andrade, Gonca Cakmak Demircigil, Aníbal Domínguez Odio, Hamiyet Donmez-Altuntas, Gilka Gattas, Ashok Giri, Sarbani Giri, Belinda Gómez-Meda, Sandra Gómez-Arroyo, Valeria Hadjidekova, Anja Haveric, Mala Kamboj, Kemajl Kurteshi, Maria Grazia Martino-Roth, Regina Montero Montoya, Armen Nersesyan, Susana Pastor-Benito, Daisy Maria Favero Salvadori, Alina Shaposhnikova, Helga Stopper, Philip Thomas, Olivia Torres-Bugarín, Abhay Singh Yadav, Guillermo Zúñiga González, Michael Fenech
The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN(XL) collaborative study. The HUMN(XL) project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells...
November 2011: Mutation Research
Michael Fenech, Nina Holland, Errol Zeiger, Wushou P Chang, Sema Burgaz, Philip Thomas, Claudia Bolognesi, Siegfried Knasmueller, Micheline Kirsch-Volders, Stefano Bonassi
The International Human Micronucleus (HUMN) Project ( was founded in 1997 to coordinate worldwide research efforts aimed at using micronucleus (MN) assays to study DNA damage in human populations. The central aims were to (i) collect databases on baseline MN frequencies and associated methodological, demographic, genetic and exposure variables, (ii) determine those variables that affect MN frequency, (iii) establish standardised protocols for performing assays so that data comparisons can be made more reliably across laboratories and countries and (iv) evaluate the association of MN frequency with disease outcomes both cross-sectionally and prospectively...
January 2011: Mutagenesis
Nina Holland, Alexandra Fucic, Domenico Franco Merlo, Radim Sram, Micheline Kirsch-Volders
Children may be more susceptible to the effects of the environmental exposure and medical treatments than adults; however, limited information is available about the differences in genotoxic effects in children by age, sex and health status. Micronucleus (MN) assay is a well established method of monitoring genotoxicity, and this approach is thoroughly validated for adult lymphocytes by the Human Micronucleus Biomonitoring project ( Similar international undertaking is in progress for exfoliated buccal cells...
January 2011: Mutagenesis
Thomas Rath, Vilma Oliveira-Frick
BACKGROUND: Immunosuppressive therapy is essential for patients after renal transplantation, but it may have mutagenic side effects. The frequency of micronuclei (MN) assessed by the cytokinesis block assay is well established in the diagnosis of DNA damage. METHODS: We examined 79 patients before and after renal transplantation with the cytokinesis block assay. For MN evaluation, the criteria of the Human Micron Nucleus (HUMN) project were used. RESULTS: Age and sex had no influence on the number of MN before transplantation...
2009: American Journal of Nephrology
Stefano Bonassi, Barbara Biasotti, Micheline Kirsch-Volders, Siegfried Knasmueller, Errol Zeiger, Sema Burgaz, Claudia Bolognesi, Nina Holland, Philip Thomas, Michael Fenech
The study of DNA damage in exfoliated buccal cells is a minimally invasive method for monitoring populations for exposure to genotoxic agents. The presence of micronuclei (MN) and other nuclear anomalies within these cells has been shown to be associated with genetic defects in genome maintenance, accelerated ageing, genotoxic damage and some degenerative diseases. To identify important information gaps regarding these biomarkers, a new initiative was launched within the framework of the HUman MicroNucleus (HUMN) collaborative programme, the HUMN(XL) project ('XL' designating eXfoLiated cell)...
July 2009: Mutagenesis
Michael Fenech
A brief account of an improbable career in the field of genetic toxicology is given, extending from my early years in Malta through a life-changing decision to study in Australia (Down Under). I describe the circumstances that led to the discovery of the cytokinesis-block micronucleus (CBMN) assay and its evolution into a cytome assay of chromosome breakage and loss (micronuclei), asymmetrical chromosome rearrangements or telomere end fusions (nucleoplasmic bridges), gene amplification (nuclear buds), cell death (necrosis, apoptosis) and cytostasis (nuclear division index)...
March 2009: Mutation Research
Michael Fenech, Nina Holland, Siegfried Knasmueller, Sema Burgaz, Stefano Bonassi
This report describes the aims, discussion and outcomes of the first Human Micronucleus collaborative project workshop on the buccal micronucleus assay. It was agreed at the workshop that three activities should be initiated as soon as possible namely (i) a method for collection of databases, (ii) writing of a protocol based on the most commonly used and best validated procedures and (iii) an inter-laboratory slide-scoring exercise in this order. A follow-up workshop is planned at the 10th International Conference on Environmental Mutagens in Florence in 2009...
March 2009: Mutagenesis
Ilse Decordier, Alexander Papine, Gina Plas, Sam Roesems, Kim Vande Loock, Jennifer Moreno-Palomo, Eduardo Cemeli, Diana Anderson, Aleksandra Fucic, Ricardo Marcos, Françoise Soussaline, Micheline Kirsch-Volders
Micronuclei (MN) frequencies in peripheral blood lymphocytes have been used worldwide as a biomarker of chromosomal damage for genotoxicity testing and biomonitoring studies. Automation of MN analysis would provide faster and more reliable results with minimizing subjective MN identification. We developed an automated facility for the scoring of the in vitro MN cytokinesis-block assay for biomonitoring on Giemsa-stained slides, fulfilling the following criteria: applicable to the cytokinesis-block micronucleus methodology, discriminating between mono-, bi- and polynucleated cells, MN scoring according to HUMN scoring criteria, false-negative MN rate <10% and false-positive (FP) MN rate <1%...
January 2009: Mutagenesis
V Garaj-Vrhovac, G Gajski, S Ravlić
The cytokinesis-block micronucleus (CBMN) assay is one of the standard cytogenetic tools employed to assess chromosomal damage subsequent to exposure to genotoxic/cytotoxic agents, and is widely applicable to plant, animal and human cells. In the present study, the CBMN assay was used to assess the baseline damage in binuclear human peripheral blood lymphocytes exposed to 25 microg/L p,p'-DDT for 1, 2, 24, and 48 h by measuring the frequency of micronuclei, nucleoplasmic bridges and nuclear buds. These new scoring criteria facilitated the detection of different types of clastogenic and aneugenic effects induced by this type of pollutant...
June 2008: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Nina Holland, Claudia Bolognesi, Micheline Kirsch-Volders, Stefano Bonassi, Errol Zeiger, Siegfried Knasmueller, Michael Fenech
The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary deficiencies, and different diseases, important knowledge gaps remain about the characteristics of micronuclei and other nuclear abnormalities, the basic biology explaining the appearance of various cell types in buccal mucosa samples and effects of diverse staining procedures and scoring criteria in laboratories around the world...
July 2008: Mutation Research
Michael Fenech, Claudia Bolognesi, Micheline Kirsch-Volders, Stefano Bonassi, Errol Zeiger, Siegfried Knasmüller, Nina Holland
No abstract text is available yet for this article.
January 2007: Mutagenesis
Stefano Bonassi, Ariana Znaor, Marcello Ceppi, Cecilia Lando, Wushou Peter Chang, Nina Holland, Micheline Kirsch-Volders, Errol Zeiger, Sadayuki Ban, Roberto Barale, Maria Paola Bigatti, Claudia Bolognesi, Antonina Cebulska-Wasilewska, Eleonora Fabianova, Alexandra Fucic, Lars Hagmar, Gordana Joksic, Antonietta Martelli, Lucia Migliore, Ekaterina Mirkova, Maria Rosaria Scarfi, Andrea Zijno, Hannu Norppa, Michael Fenech
The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) is extensively used as a biomarker of chromosomal damage and genome stability in human populations. Much theoretical evidence has been accumulated supporting the causal role of MN induction in cancer development, although prospective cohort studies are needed to validate MN as a cancer risk biomarker. A total of 6718 subjects from of 10 countries, screened in 20 laboratories for MN frequency between 1980 and 2002 in ad hoc studies or routine cytogenetic surveillance, were selected from the database of the HUman MicroNucleus (HUMN) international collaborative project and followed up for cancer incidence or mortality...
March 2007: Carcinogenesis
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