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Jie-Lin Wang, Qian Chen, Bei-Bei Du, Lu Cao, Hong Lin, Zhong-Yong Fan, Jian Dong
The composite polylactide PLLA/β-TCP scaffolds were fabricated by solution casting and were coated with gelatin/hydroxyapatite (Gel/HAp) to improve the biological properties of the composite scaffolds. The Gel/HAp mixture was prepared using an in situ reaction, and a grafting-coating method was used to increase the efficiency of coating the PLLA/β-TCP matrix with Gel/HAp. First, free amino groups were introduced by 1,6-hexanediamine to aminolyze the PLLA/β-TCP matrix surface. Second, glutaraldehyde was coupled to Gel/HAp as a crosslinking agent...
June 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
James Buxbaum, Michael Quezada, Bradford Chong, Nikhil Gupta, Chung Yao Yu, Christianne Lane, Ben Da, Kenneth Leung, Ira Shulman, Stephen Pandol, Bechien Wu
OBJECTIVES: The Pancreatitis Activity Scoring System (PASS) has been derived by an international group of experts via a modified Delphi process. Our aim was to perform an external validation study to assess for concordance of the PASS score with high face validity clinical outcomes and determine specific meaningful thresholds to assist in application of this scoring system in a large prospectively ascertained cohort. METHODS: We analyzed data from a prospective cohort study of consecutive patients admitted to the Los Angeles County Hospital between March 2015 and March 2017...
March 15, 2018: American Journal of Gastroenterology
Wei Du, Xinyue Li, Yuanchen Chen, Guofeng Shen
Solid fuels, an important source of severe Household Air Pollution (HAP) linked to many adverse health outcomes, has been widely consumed around the world. China consumes large amounts of solid fuels and suffers from serious indoor and outdoor air pollution. Though global HAP issues had been reviewed in previous literatures, peer-reviewed Chinese publications were seldom included in those reviews. We conducted a literature review on the studies of HAP and personal exposure in rural China with inputs from peer-reviewed publications in both English and Chinese...
March 8, 2018: Environmental Pollution
Feng-Li He, Da-Wei Li, Jin He, Yang-Yang Liu, Fiaz Ahmad, Ya-Li Liu, Xudong Deng, Ya-Jing Ye, Da-Chuan Yin
Electrospinning is a powerful method for preparing porous materials that can be applied as biomedical materials for implantation or tissue engineering or as scaffolds for 3D cell culture experiments. However, this technique is limited in practical applications because the pore size of 3D scaffolds directly prepared by conventional electrospinning is usually less than several tens of micrometres, which may not be suitable for 3D cell culture and tissue growth. To allow for satisfactory 3D cell culture and tissue engineering, the pore size of the scaffold should be controllable according to the requirement of the specific cells to be cultured...
May 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Baojin Ma, Jing Han, Shan Zhang, Feng Liu, Shicai Wang, Jiazhi Duan, Yuanhua Sang, Huaidong Jiang, Dong Li, Shaohua Ge, Jinghua Yu, Hong Liu
Controllable osteoinduction maintained in the original defect area is the key to precise bone repair. To meet the requirement of precise bone regeneration, a hydroxyapatite (HAp) nanobelt/polylactic acid (PLA) (HAp/PLA) Janus membrane has been successfully prepared in this study by coating PLA on a paper-like HAp nanobelt film by a casting-pervaporation method. The Janus membrane possesses dual functions: excellent osteoinduction from the hydrophilic HAp nanobelt side and barrier function originating from the hydrophobic PLA film...
March 7, 2018: Acta Biomaterialia
Klaudia Pluta, Agnieszka Sobczak-Kupiec, Olga Półtorak, Dagmara Malina, Bożena Tyliszczak
Calcium phosphates (CPs) constitute attractive materials of biomedical applications. Among them particular attention is devoted to bioactive hydroxyapatite (HAp) and bioresorbable tricalcium phosphate (TCP) that possess ability to bind to living bones and can be used clinically as important bone substitutes. Notably, in vivo bone bioactivity can be predicted from apatite formation of bone immersed in SBF fluids. Thus, analyses of behavior of calcium phosphates immersed in various bio fluids are of great importance...
March 9, 2018: Journal of Biomedical Materials Research. Part A
Lia R Valeeva, Chuluuntsetseg Nyamsuren, Margarita R Sharipova, Eugene V Shakirov
Phytases are specialized phosphatases capable of releasing inorganic phosphate from myo -inositol hexakisphosphate (phytate), which is highly abundant in many soils. As inorganic phosphorus reserves decrease over time in many agricultural soils, genetic manipulation of plants to enable secretion of potent phytases into the rhizosphere has been proposed as a promising approach to improve plant phosphorus nutrition. Several families of biotechnologically important phytases have been discovered and characterized, but little data are available on which phytase families can offer the most benefits toward improving plant phosphorus intake...
2018: Frontiers in Plant Science
Teruaki Nakatsuji, Tiffany H Chen, Anna M Butcher, Lynnie L Trzoss, Sang-Jip Nam, Karina T Shirakawa, Wei Zhou, Julia Oh, Michael Otto, William Fenical, Richard L Gallo
We report the discovery that strains of Staphylococcus epidermidis produce 6- N -hydroxyaminopurine (6-HAP), a molecule that inhibits DNA polymerase activity. In culture, 6-HAP selectively inhibited proliferation of tumor lines but did not inhibit primary keratinocytes. Resistance to 6-HAP was associated with the expression of mitochondrial amidoxime reducing components, enzymes that were not observed in cells sensitive to this compound. Intravenous injection of 6-HAP in mice suppressed the growth of B16F10 melanoma without evidence of systemic toxicity...
February 2018: Science Advances
S Hadj Rabia, F Benmoussa, A Benzaid, A Baz
The present preliminary study intends to evaluate the in vitro use of hydatid antigen and their antibodies once labeled with iodine 125(I125 ) and characterized from viewpoint of radiochemical purity and immunoreactivity. Radiolabelled molecules gave satisfactory purity of 94% and 96%-98%, for hydatid antigen and IgG respectively. As regards, the specific activity of these latter, varied between 4.79 and 5.97 μCi/μg. The specificity test of radiolabelled IgG against the hydatid membranes showed a significant recognition that increased proportionally according to the contact surface...
February 27, 2018: Experimental Parasitology
July Andrea Rincón-López, Jennifer Andrea Hermann-Muñoz, Astrid Lorena Giraldo-Betancur, Andrea De Vizcaya-Ruiz, Juan Manuel Alvarado-Orozco, Juan Muñoz-Saldaña
The physicochemical properties and biological behavior of sintered-bovine-derived hydroxyapatite (BHAp) are here reported and compared to commercial synthetic-HAp (CHAp). Dense ceramics were sintered for 2 h and 4 h at 1200 °C to investigate their microstructure-structure-in-vitro behavior relationship for both HAp ceramics. Densification was directly proportional to sintering time, showing a grain coarsening behavior with a greater effect on BHAp. Lattice parameters, crystallite size, cell volume and Ca / P ratio were determined by Rietveld refinement of X-ray diffraction (XRD) patterns using GSAS® ...
February 25, 2018: Materials
Clara J Bohm, Leroy J Storsley, Brett M Hiebert, Serena Nelko, Navdeep Tangri, Lawrence J Cheskin, Mara A McAdams-DeMarco, Claudio Rigatto
Background: Individuals with chronic kidney disease (CKD) have low levels of physical activity and physical function. Although guidelines endorse exercise counseling for individuals with CKD, it is not yet part of routine care. Objective: We investigated the effect of attending a real-life exercise counseling clinic (ECC) on physical function in individuals with CKD. Design: Retrospective analysis of prospectively collected observational data with quasi-experimental design...
2018: Canadian Journal of Kidney Health and Disease
Huma Ajab, John Ojur Dennis, Mohd Azmuddin Abdullah
A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum. The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration. The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding...
February 24, 2018: International Journal of Biological Macromolecules
Elham Gholibegloo, Ashkan Karbasi, Maryam Pourhajibagher, Nasim Chiniforush, Ali Ramazani, Tayebeh Akbari, Abbas Bahador, Mehdi Khoobi
Antimicrobial photodynamic therapy (aPDT) has been emerged as a noninvasive strategy to remove bacterial contaminants such as S. mutans from the tooth surface. Photosensitizer (PS), like indocyanine green (ICG), plays a key role in this technique which mainly suffers from the poor stability and concentration-dependent aggregation. An appropriate nanocarrier (NC) with enhanced antibacterial effects could overcome these limitations and improve the efficiency of ICG as a PS. In this study, various ICG-loaded NCs including graphene oxide (GO), GO-carnosine (Car) and GO-Car/Hydroxyapatite (HAp) were synthesized and characterized by Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Filed Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDS), Zeta Potential and Ultraviolet-Visible spectrometry (UV-Vis)...
February 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
Sahar Elkholy, Sarah Yahia, Manal Awad, Medhat Elmessiery
BACKGROUND: Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering. Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap. PURPOSE: β-Chitosan/n-HA composite with different percentages was prepared. Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope). Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done...
February 26, 2018: Clinical Implant Dentistry and related Research
Jennifer Haynes, Trevor D McKee, Andrew C Haller, Yadong Wang, Cherry Leung, Deena M A Gendoo, Evelyne Lima-Fernandes, Antonia Kreso, Robin Wolman, Eva Szentgyorgyi, Douglass C Vines, Benjamin Haibe-Kains, Bradly G Wouters, Ur Metser, David A Jaffray, Myles Smith, Catherine A O'Brien
PURPOSE: Cancer-initiating cells (C-ICs) have been described in multiple cancer types, including colorectal cancer (CRC). C-ICs are defined by their capacity to self-renew, thereby driving tumor growth. C-ICs were initially thought to be static entities, however recent studies have determined these cells to be dynamic and influenced by microenvironmental cues such as hypoxia. If hypoxia drives the formation of C-ICs, then targeting hypoxia could represent a novel means to target C-ICs...
February 23, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Olga A Kladova, Milena Bazlekowa-Karaban, Sonia Baconnais, Olivier Piétrement, Alexander A Ishchenko, Bakhyt T Matkarimov, Danila A Iakovlev, Andrey Vasenko, Olga S Fedorova, Eric Le Cam, Barbara Tudek, Nikita A Kuznetsov, Murat Saparbaev
The base excision repair (BER) pathway consists of sequential action of DNA glycosylase and apurinic/apyrimidinic (AP) endonuclease necessary to remove a damaged base and generate a single-strand break in duplex DNA. Human multifunctional AP endonuclease 1 (APE1, a.k.a. APEX1, HAP-1, or Ref-1) plays essential roles in BER by acting downstream of DNA glycosylases to incise a DNA duplex at AP sites and remove 3'-blocking sugar moieties at DNA strand breaks. Human 8-oxoguanine-DNA glycosylase (OGG1), methyl-CpG-binding domain 4 (MBD4, a...
February 11, 2018: DNA Repair
Elvire Le Norcy, Julie Lesieur, Jeremy Sadoine, Gaël Y Rochefort, Catherine Chaussain, Anne Poliard
The Leucine Rich Amelogenin Peptide (LRAP) is a product of alternative splicing of the amelogenin gene. As full length amelogenin, LRAP has been shown, in precipitation experiments, to regulate hydroxyapatite (HAP) crystal formation depending on its phosphorylation status. However, very few studies have questioned the impact of its phosphorylation status on enamel mineralization in biological models. Therefore, we have analyzed the effect of phosphorylated (+P) or non-phosphorylated (-P) LRAP on enamel formation in ameloblast-like cell lines and ex vivo cultures of murine postnatal day 1 molar germs...
2018: Frontiers in Physiology
H D A Chathumal Jayaweera, Induni Siriwardane, K M Nalin de Silva, Rohini M de Silva
Clean water, which is free from pathogens and toxic chemicals, is vital to human health. The blue planet is encountering remarkable challenges in meeting the ever-increasing demands of clean water. The intention of this research study was to develop a water filter material that is capable of removing bacterial contaminants and heavy metals from fresh water using cost effective and easily fabricated biocompatible filter material. For this purpose, granular activated carbon (GAC) was coated with both hydroxyapatite (HAP) nanoflakes and turmeric extract (TE) (HAP/TE/GAC) which had been extracted from natural turmeric powder...
February 21, 2018: Chemistry Central Journal
Alessandra Gallinaro, Martina Borghi, Roberta Bona, Felicia Grasso, Laura Calzoletti, Laura Palladino, Serena Cecchetti, Maria Fenicia Vescio, Daniele Macchia, Valeria Morante, Andrea Canitano, Nigel Temperton, Maria Rita Castrucci, Mirella Salvatore, Zuleika Michelini, Andrea Cara, Donatella Negri
Viral vectors represent an attractive technology for vaccine delivery. We exploited the integrase defective lentiviral vector (IDLV) as a platform for delivering relevant antigens within the context of the ADITEC collaborative research program. In particular, Influenza virus hemagglutinin (HA) and nucleoprotein (NP) were delivered by IDLVs while H1N1 A/California/7/2009 subunit vaccine (HAp) with or without adjuvant was used to compare the immune response in a murine model of immunization. In order to maximize the antibody response against HA, both IDLVs were also pseudotyped with HA (IDLV-HA/HA and IDLV-NP/HA, respectively)...
2018: Frontiers in Immunology
Honglin Luo, Jiaojiao Dong, Zhiwei Yang, Guifu Zuo, Wei Li, Junping Tu, Yizao Wan
Compared to two-dimensional (2D) conditions, investigation on gene transfection in three-dimensional (3D) conditions is much less extensive. In this work, lamellar nano-hydroxyapatite (L-HAp) with and without magnetism were used as the vectors and gene transfection in 2D and 3D cell culture was carried out and compared. We found that the transfection efficiency in 3D conditions was much higher than 2D cell culture. Additionally, magnetism enhanced transfection efficiency under both 2D and 3D conditions. The findings presented in this study demonstrated that the magnetic L-HAp could be a promising vector in 3D gene transfection...
August 1, 2018: Journal of Nanoscience and Nanotechnology
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