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https://www.readbyqxmd.com/read/29783633/butyrylation-of-maize-and-potato-starches-and-characterization-of-the-products-by-nuclear-magnetic-resonance-and-in-vitro-fermentation
#1
Tina Skau Nielsen, Nuria Canibe, Flemming Hofmann Larsen
Intake of butyrylated starches may increase colonic butyrate supply, which can be of public health and clinical benefit by maintaining colonic health. The objective was to investigate if an organocatalytic method with tartaric acid as a catalyst could be applied to produce butyrylated products from different starch sources and to characterize their chemical structure and fermentation capability by using solid-state 13 C MAS NMR (magic angle spinning nuclear magnetic resonance) spectroscopy and an in vitro fermentation model, respectively...
May 18, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29774065/catalytic-hairpin-assembly-gel-assay-for-multiple-and-sensitive-microrna-detection
#2
Wenhao Dai, Jing Zhang, Xiangdan Meng, Jie He, Kai Zhang, Yu Cao, Dongdong Wang, Haifeng Dong, Xueji Zhang
As important modulators of gene expression, microRNAs (miRNAs) have been identified as promising biomarkers with powerful predictive value in diagnosis and prognosis for several diseases, especially for cancers. Here we report a facile, multiple and sensitive miRNA detection method that uses conventional gel electrophoresis and catalytic hairpin assembly (CHA) system without any complex nanomaterials or enzymatic amplification. Methods: In this study, three pairs of hairpin probes are rationally designed with thermodynamically and kinetically preferable feasibility for the CHA process...
2018: Theranostics
https://www.readbyqxmd.com/read/29768888/effect-of-surfactants-on-the-structure-and-adsorption-efficiency-of-hydroxyapatite-nanorods
#3
Manisha Sharma, Amit Mishra, Akansha Mehta, Diptiman Choudhury, Soumen Basu
Porous hydroxyapatite (HAp) nanorods using surfactant templating proceeded via microwave irradiation method. Study of BET surface area measurement of the HAp nanorods showed that surface area of HAp nanorods with a mixture of cat-anionic surfactants was higher (56.16 m2/g) than their individual counterpart (for anionic 52.8 m2/g and for cationic 48.8 m2/g) as well as without surfactant (19.07 m2/g) due to higher synergistic effect and low critical aggregation concentration of the mixture. Surfactant-directed synthesis of porous HAp has been explored in literature, but the relation between the pore size distribution, surface area and morphology and choice of surfactant(s) was not fully understood and hence in this work we have explored those parameters...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29763771/influence-of-ph-on-wet-synthesis-of-silver-decorated-hydroxyapatite-nanopowder
#4
P Narendran, Archana Rajendran, Mirna Garhnayak, Lokanath Garhnayak, J Nivedhitha, K Chandra Devi, Deepak K Pattanayak
Here, effect of solution pH on precipitation of silver decorated hydroxyapatite (Ag-HAp) nano powder during its wet-synthesis was systematically studied. XRD pattern of Ag-HAp nano powder synthesised at pH ranging from 5 to 10 shows that calcium hydrogen phosphate was formed as dominating phase when the solution pH was between 5 and 9 and this phase was gradually transformed into a stable HAp above pH 9. A quantitative analysis of silver amount in Ag-HAp nano powder synthesised at different pH showed that silver can be precipitated to its maximum amount at pH 8 and the further addition of ammonia leads to the formation of a silver-ammonium complex, thereby remaining in the solution...
April 22, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29752857/prevalence-and-risk-factors-of-metabolic-syndrome-in-hiv-infected-adults-at-three-urban-clinics-in-a-post-conflict-setting-eastern-democratic-republic-of-congo
#5
Patrick D M C Katoto, Friedrich Thienemann, André N H Bulabula, Tonya M Esterhuizen, Aimé B Murhula, Pierre P M Lunjwire, Dieudonné M Bihehe, Jean B Nachega
OBJECTIVE: To determine the prevalence of and risk factors for metabolic syndrome (MS) in HIV-infected adults at three urban clinics in Bukavu, Democratic Republic of the Congo. DESIGN: Cross-sectional study. METHODS: From July to September 2016, baseline socio-demographics, risk factors, and clinical characteristics were collected using a structured questionnaire or extracted from medical records. Fasting blood sugar and lipids were measured...
May 12, 2018: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29752109/the-effect-of-the-androstane-lung-cancer-inhibitor-content-on-the-cell-selective-toxicity-of-hydroxyapatite-chitosan-plga-nanocomposites
#6
Nenad L Ignjatović, Katarina M Penov-Gaši, Jovana J Ajduković, Vesna V Kojić, Smilja B Marković, Dragan P Uskoković
An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29750989/altered-ca-2-signaling-in-enamelopathies
#7
REVIEW
Miriam Eckstein, Francisco J Aulestia, Meerim K Nurbaeva, Rodrigo S Lacruz
Biomineralization requires the controlled movement of ions across cell barriers to reach the sites of crystal growth. Mineral precipitation occurs in aqueous phases as fluids become supersaturated with specific ionic compositions. In the biological world, biomineralization is dominated by the presence of calcium (Ca2+ ) in crystal lattices. Ca2+ channels are intrinsic modulators of this process, facilitating the availability of Ca2+ within cells in a tightly regulated manner in time and space. Unequivocally, the most mineralized tissue produced by vertebrates, past and present, is dental enamel...
May 8, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29750799/population-specific-genetic-modification-of-huntington-s-disease-in-venezuela
#8
Michael J Chao, Kyung-Hee Kim, Jun Wan Shin, Diane Lucente, Vanessa C Wheeler, Hong Li, Jared C Roach, Leroy Hood, Nancy S Wexler, Laura B Jardim, Peter Holmans, Lesley Jones, Michael Orth, Seung Kwak, Marcy E MacDonald, James F Gusella, Jong-Min Lee
Modifiers of Mendelian disorders can provide insights into disease mechanisms and guide therapeutic strategies. A recent genome-wide association (GWA) study discovered genetic modifiers of Huntington's disease (HD) onset in Europeans. Here, we performed whole genome sequencing and GWA analysis of a Venezuelan HD cluster whose families were crucial for the original mapping of the HD gene defect. The Venezuelan HD subjects develop motor symptoms earlier than their European counterparts, implying the potential for population-specific modifiers...
May 11, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29749273/enhanced-osteogenic-activity-with-boron-doped-nanohydroxyapatite-loaded-poly-butylene-adipate-co-terephthalate-fibrous-3d-matrix
#9
Aysu Arslan, Soner Çakmak, Menemşe Gümüşderelioğlu
In this study, three dimensional (3D) poly(butylene adipate-co-terephthalate) (PBAT) fibrous scaffolds with more than 90% porosity were fabricated via wet electrospinning method. Amorphous hydroxyapatite (HAp) and boron (B) doped hydroxyapatite (B-HAp) nanoparticles were produced by microwave-assisted biomimetic precipitation and encapsulated into PBAT fibres with the ratio of 5% (w/w) in order to enhance osteogenic activity of the scaffolds. Cell culture studies were carried out with human bone marrow derived stem cells (hBMSCs) and they showed that alkaline phosphatase (ALP) activity and the amounts of collagen and calcium were higher on B containing PBAT (B-HAp-PBAT) scaffolds during the 28-day culture period than that of the PBAT scaffolds...
May 11, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29741076/monitoring-of-hospital-acquired-pneumonia-in-patients-with-severe-brain-injury-on-first-access-to-intensive-neurological-rehabilitation-first-year-of-observation
#10
Gianfranco Beghi, Antonio De Tanti, Paolo Serafini, Chiara Bertolino, Antonietta Celentano, Graziella Taormina
Nosocomial or hospital acquired pneumonia (HAP) is an illness contracted during a hospital stay, generally with onset 48 hours or more after admission to hospital, or within 14 days of discharge from hospital. HAP is divided into subgroups: Ventilator-associated pneumonia (VAP), accounting for 86% of hospital acquired pneumonia, and stroke-associated pneumonia (SAP). The incidence of SAP in neurological intensive care units (NICUs) is 4.1-56.6%, in medical intensive care units (MICUs) it is 17-50%, in stroke units it is 3...
May 9, 2018: Monaldi Archives for Chest Disease, Archivio Monaldi Per le Malattie del Torace
https://www.readbyqxmd.com/read/29739509/ultrasonic-assisted-conversion-of-limestone-into-needle-like-hydroxyapatite-nanoparticles
#11
Jutharatana Klinkaewnarong, Songkot Utara
Needle-like hydroxyapatite nanoparticles were successfully synthesized via a reaction between calcium oxide (CaO) that was obtained from calcined limestone and orthophosphoric acid (H3 PO4 ) under ultrasonic irradiation at 25 °C. The reaction systems were exposed to ultrasonic waves of 20 kHz for various times ranging from 0 to 4 h. The initial and final pH values of the mixtures of CaO and H3 PO4 solution were continuously observed (pH < 4.0) after ultrasonic irradiation. The powder was then dried at 60 °C and calcined at 300 °C for 3 h (3 °C/min)...
September 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29738539/gene-dosage-effects-in-yeast-support-broader-roles-for-the-log1-ham1-and-dut1-genes-in-detoxification-of-nucleotide-analogues
#12
Mattias Carlsson, Guo-Zhen Hu, Hans Ronne
Purine and pyrimidine analogues have important uses in chemotherapies against cancer, and a better understanding of the mechanisms that cause resistance to these drugs is therefore of importance in cancer treatment. In the yeast Saccharomyces cerevisiae, overexpression of the HAM1 gene encoding inosine triphosphate pyrophosphatase confers resistance to both the purine analogue 6-N-hydroxylaminopurine (HAP) and the pyrimidine analogue 5-fluorouracil (5-FU) (Carlsson et al., 2013, PLoS One 8, e52094). To find out more about the mechanisms of resistance to nucleotide analogues, and possible interdependencies between purine and pyrimidine analogue resistance mechanisms, we screened a plasmid library in yeast for genes that confer HAP resistance when overexpressed...
2018: PloS One
https://www.readbyqxmd.com/read/29731584/fuel-efficiency-and-air-pollutant-concentrations-of-wood-burning-improved-cookstoves-in-malawi-implications-for-scaling-up-cookstove-programs
#13
Pamela Jagger, Joseph Pedit, Ashley Bittner, Laura Hamrick, Tione Phwandapwhanda, Charles Jumbe
National governments and other key stakeholders in developing countries are grappling with how to reduce household air pollution (HAP) resulting from cooking with solid fuels using traditional cooking technologies. Recent studies have shown that improved cookstoves may offer reductions in fuel use and harmful emissions of carbon monoxide (CO) and fine particulate matter (PM2.5 ), yet there is little quantitative evidence collected in a "real-world" setting showing how improved stoves perform directly compared to traditional cooking technologies...
December 2017: Energy for Sustainable Development: the Journal of the International Energy Initiative
https://www.readbyqxmd.com/read/29722052/the-methodological-quality-of-guidelines-for-hospital-acquired-pneumonia-and-ventilator-associated-pneumonia-a-systematic-review
#14
REVIEW
R Ambaras Khan, Z Aziz
WHAT IS KNOWN AND OBJECTIVES: Clinical practice guidelines serve as a framework for physicians to make decisions and to support best practice for optimizing patient care. However, if the guidelines do not address all the important components of optimal care sufficiently, the quality and validity of the guidelines can be reduced. The objectives of this study were to systematically review current guidelines for hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP), evaluate their methodological quality and highlight the similarities and differences in their recommendations for empirical antibiotic and antibiotic de-escalation strategies...
May 2, 2018: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/29721617/the-effect-of-cyclic-heat-treatment-on-the-physicochemical-properties-of-bio-hydroxyapatite-from-bovine-bone
#15
S M Londoño-Restrepo, R Jeronimo-Cruz, E Rubio-Rosas, M E Rodriguez-García
This paper focus on physicochemical changes in bio-hydroxyapatite (BIO-HAp) from bovine femur obtained by calcination at high temperatures: 520-620 (each 20 °C) at 7.4 °C/min and from 700 to 1100 °C (each 100 °C) at three heating rates: 7.4, 9.9, and 11.1 °C/min. BIO-HAp samples were obtained using a multi-step process: cleaning, milling, hydrothermal process, calcination in an air atmosphere, and cooling in furnace air. Inductively Couple Plasma (ICP) showed that the presence of Mg, K, S, Ba, Zn, and Na, is not affected by the annealing temperature and heating rate...
May 2, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29721565/capturing-the-effect-of-pf-3-c-2-f-5-3-vs-pf-6-flexible-anion-vs-rigid-and-scaled-charge-vs-unit-on-the-transport-properties-of-bmim-based-ionic-liquids-a-comparative-md-study
#16
Mohammad H Kowsari, Soraya Ebrahimi
Comprehensive molecular dynamics simulations are performed to study the average single-particle dynamics and the transport properties of 1-butyl-3-methylimidazolium hexafluorophosphate, [bmim][PF6], and 1-butyl-3-methylimidazolium tris(pentafluoroethyl)trifluorophosphate, [bmim][FAP], ionic liquids (ILs) at 400 K. We applied one of the most widely used nonpolarizable all-atom force fields for ILs, both with the original unit (±1) charges on each ion and with the partial charges uniformly scaled to 80-85%, taking into account the average polarizability and tracing the experimentally compatible transport properties...
May 3, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29716740/chemical-interaction-of-glycero-phosphate-dimethacrylate-gpdm-with-hydroxyapatite-and-dentin
#17
Kumiko Yoshihara, Noriyuki Nagaoka, Satoshi Hayakawa, Takumi Okihara, Yasuhiro Yoshida, Bart Van Meerbeek
OBJECTIVES: Although the functional monomer glycero-phosphate dimethacrylate (GPDM) has since long been used in several dental adhesives and more recently in self-adhesive composite cements and restoratives, its mechanism of chemical adhesion to hydroxyapatite (HAp) is still unknown. We therefore investigated the chemical interaction of GPDM with HAp using diverse chemical analyzers and ultra-structurally characterized the interface of a GPDM-based primer formulation with dentin. METHODS: HAp particles were added to a GPDM solution for various periods, upon which they were thoroughly washed with ethanol and water prior to being air-dried...
April 28, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29710862/antimicrobial-activity-of-new-materials-based-on-lavender-and-basil-essential-oils-and-hydroxyapatite
#18
Daniela Predoi, Simona Liliana Iconaru, Nicolas Buton, Monica Luminita Badea, Luminita Marutescu
This study presents, for the first-time, the results of a study on the hydrodynamic diameter of essential oils (EOs) of basil and lavender in water, and solutions of EOs of basil (B) and lavender (L) and hydroxyapatite (HAp). The possible influence of basil and lavender EOs on the size of hydroxyapatite nanoparticles was analyzed by Scanning Electron Microscopy (SEM). We also investigated the in vitro antimicrobial activity of plant EOs and plant EOs hydroxyapatite respectively, against Gram-positive bacteria (methicillin-resistant Staphylococcus aureus 1144 (MRSA 1144) and S...
April 30, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29710502/osteoblast-response-to-vitamin-d3-loaded-cellulose-enriched-hydroxyapatite-mesoporous-silica-nanoparticles-composite
#19
Murugan Sumathra, Murugan A Munusamy, Abdulla A Alarfaj, Mariappan Rajan
Composite materials hold cellulose(C) established network and biomimetic Hydroxyapatite (HAP) are potentially appropriate for bone formation and protect the external organisms. Tissue engineering scaffolds with unified porous and enhanced biological properties can be fabricated using a small quantity of porous additives. In this study we synthesizd a Cholecalciferol (VD3) loaded, cellulose functionalized hydroxyapatite nanocomposites with different concentration of Mesoporous silica nanoparticles (MSNs). VD3 plays an important role in the bone formation by regulating extracellular levels of calcium and phosphorus...
April 21, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29707852/dentinal-tubule-occluding-capability-of-nano-hydroxyapatite-the-in-vitro-evaluation
#20
Serdar Baglar, Umit Erdem, Mustafa Dogan, Mustafa Turkoz
In this in-vitro study, the effectiveness of experimental pure nano-hydroxyapatite (nHAP) and 1%, 2%, and 3% F¯ doped nano-HAp on dentine tubule occlusion was investigated. And also, the cytotoxicity of materials used in the experiment was evaluated. Nano-HAp types were synthesized by the precipitation method. Forty dentin specimens were randomly divided into five groups of; 1-no treatment (control), 2-specimens treated with 10% pure nano-HAp and 3, 4, 5 specimens treated with 1%, 2%, and 3% F- doped 10% nano-HAp, respectively...
April 29, 2018: Microscopy Research and Technique
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