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Piezo1

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https://www.readbyqxmd.com/read/29757938/piezo1-channel-is-a-potential-regulator-of-synovial-sarcoma-cell-viability
#1
Takahisa Suzuki, Yukiko Muraki, Noriyuki Hatano, Hiroka Suzuki, Katsuhiko Muraki
Detection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone. PIEZO1, a mechano-sensing cation channel, is widely expressed in neuronal and non-neuronal cells and is expected to be involved in important biological functions. Here, we examined the possibility that PIEZO1 is involved in the regulation of synovial sarcoma cell-viability. Application of a PIEZO1 agonist Yoda1 effectively induced Ca2+ response and cation channel currents in PIEZO1 -expressing HEK (HEK-Piezo1) cells and synovial sarcoma SW982 (SW982) cells...
May 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29755372/use-of-laser-assisted-optical-rotational-cell-analyzer-lorrca-maxsis-in-the-diagnosis-of-rbc-membrane-disorders-enzyme-defects-and-congenital-dyserythropoietic-anemias-a-monocentric-study-on-202-patients
#2
Anna Zaninoni, Elisa Fermo, Cristina Vercellati, Dario Consonni, Anna P Marcello, Alberto Zanella, Agostino Cortelezzi, Wilma Barcellini, Paola Bianchi
Chronic hemolytic anemias are a group of heterogeneous diseases mainly due to abnormalities of red cell (RBC) membrane and metabolism. The more common RBC membrane disorders, classified on the basis of blood smear morphology, are hereditary spherocytosis (HS), elliptocytosis, and hereditary stomatocytoses (HSt). Among RBC enzymopathies, the most frequent is pyruvate kinase (PK) deficiency, followed by glucose-6-phosphate isomerase, pyrimidine 5' nucleotidase P5'N, and other rare enzymes defects. Because of the rarity and heterogeneity of these diseases, diagnosis may be often challenging despite the availability of a variety of laboratory tests...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29735991/increased-piezo1-channel-activity-in-interstitial-cajal-like-cells-induces-bladder-hyperactivity-by-functionally-interacting-with-ncx1-in-rats-with-cyclophosphamide-induced-cystitis
#3
Qian Liu, Bishao Sun, Jiang Zhao, Qingqing Wang, Fan An, Xiaoyan Hu, Zhenxing Yang, Jie Xu, Mingjia Tan, Longkun Li
The Piezo1 channel is a mechanotransduction mediator, and Piezo1 abnormalities have been linked to several clinical disorders. However, the role of the Piezo1 channel in cystitis-associated bladder dysfunction has not been documented. The current study aimed to discover the functional role of this channel in regulating bladder activity during cyclophosphamide (CYP)-induced cystitis. One hundred four female rats were randomly assigned to the control, CYP-4h, CYP-48h and CYP-8d groups. CYP successfully induced acute or chronic cystitis in these rats...
May 7, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29713289/the-molecular-basis-for-altered-cation-permeability-in-hereditary-stomatocytic-human-red-blood-cells
#4
REVIEW
Joanna F Flatt, Lesley J Bruce
Normal human RBCs have a very low basal permeability (leak) to cations, which is continuously corrected by the Na,K-ATPase. The leak is temperature-dependent, and this temperature dependence has been evaluated in the presence of inhibitors to exclude the activity of the Na,K-ATPase and NaK2Cl transporter. The severity of the RBC cation leak is altered in various conditions, most notably the hereditary stomatocytosis group of conditions. Pedigrees within this group have been classified into distinct phenotypes according to various factors, including the severity and temperature-dependence of the cation leak...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29712913/piezo1-is-a-mechanically-activated-ion-channel-and-mediates-pressure-induced-pancreatitis
#5
Joelle M-J Romac, Rafiq A Shahid, Sandip M Swain, Steven R Vigna, Rodger A Liddle
Merely touching the pancreas can lead to premature zymogen activation and pancreatitis but the mechanism is not completely understood. Here we demonstrate that pancreatic acinar cells express the mechanoreceptor Piezo1 and application of pressure within the gland produces pancreatitis. To determine if this effect is through Piezo1 activation, we induce pancreatitis by intrapancreatic duct instillation of the Piezo1 agonist Yoda1. Pancreatitis induced by pressure within the gland is prevented by a Piezo1 antagonist...
April 30, 2018: Nature Communications
https://www.readbyqxmd.com/read/29709295/activation-of-mechanosensitive-transient-receptor-potential-piezo-channels-in-odontoblasts-generates-action-potentials-in-cocultured-isolectin-b-4-negative-medium-sized-trigeminal-ganglion-neurons
#6
Masaki Sato, Kazuhiro Ogura, Maki Kimura, Koichi Nishi, Masayuki Ando, Masakazu Tazaki, Yoshiyuki Shibukawa
INTRODUCTION: Various stimuli to the dentin surface elicit dentinal pain by inducing dentinal fluid movement causing cellular deformation in odontoblasts. Although odontoblasts detect deformation by the activation of mechanosensitive ionic channels, it is still unclear whether odontoblasts are capable of establishing neurotransmission with myelinated A delta (Aδ) neurons. Additionally, it is still unclear whether these neurons evoke action potentials by neurotransmitters from odontoblasts to mediate sensory transduction in dentin...
April 27, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29689211/structural-biology-piezo-senses-tension-through-curvature
#7
Xin Liang, Jonathon Howard
The sensations of sound, touch and pressure are mediated by mechanotransduction channels - transmembrane proteins whose ionic permeabilities are gated by mechanical forces. New structures of Piezo1 by cryoEM lead to the suggestion that this channel might sense membrane tension through changes in the local curvature of the membrane.
April 23, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29683214/piezo1-functions-as-a-potential-oncogene-by-promoting-cell-proliferation-and-migration-in-gastric-carcinogenesis
#8
Jinglin Zhang, Yuhang Zhou, Tingting Huang, Feng Wu, Liping Liu, Johnny S H Kwan, Alfred S L Cheng, Jun Yu, Ka Fai To, Wei Kang
Gastric cancer (GC) is one of the most common malignancies in Asian areas. PIEZO1 has been implied to regulate epithelial homeostasis to play an important role in physiological processes. It is also related to tumor initiation and progression. However, the role of PIEZO1 has not yet been explored in GC. The expression of PIEZO1 in GC cell lines and primary samples was determined by qRT-PCR and Western blot. The clinical correlation of PIEZO1 in GC was evaluated by immunohistochemistry on tissue microarray. The oncogenic role of PIEZO1 was demonstrated in gastric tumorigenesis through a series of functional assays, including cell proliferation, cell invasion, and flow cytometry analysis...
April 23, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29669276/tmem150c-tentonin3-is-a-regulator-of-mechano-gated-ion-channels
#9
Evan O Anderson, Eve R Schneider, Jon D Matson, Elena O Gracheva, Sviatoslav N Bagriantsev
Neuronal mechano-sensitivity relies on mechano-gated ion channels, but pathways regulating their activity remain poorly understood. TMEM150C was proposed to mediate mechano-activated current in proprioceptive neurons. Here, we studied functional interaction of TMEM150C with mechano-gated ion channels from different classes (Piezo2, Piezo1, and the potassium channel TREK-1) using two independent methods of mechanical stimulation. We found that TMEM150C significantly prolongs the duration of the mechano-current produced by all three channels, decreases apparent activation threshold in Piezo2, and induces persistent current in Piezo1...
April 17, 2018: Cell Reports
https://www.readbyqxmd.com/read/29614632/sickle-cell-dehydration-pathophysiology-and-therapeutic-applications
#10
Carlo Brugnara
Cell dehydration is a distinguishing characteristic of sickle cell disease and an important contributor to disease pathophysiology. Due to the unique dependence of Hb S polymerization on cellular Hb S concentration, cell dehydration promotes polymerization and sickling. In double heterozygosis for Hb S and C (SC disease) dehydration is the determining factor in disease pathophysiology. Three major ion transport pathways are involved in sickle cell dehydration: the K-Cl cotransport (KCC), the Gardos channel (KCNN4) and Psickle, the polymerization induced membrane permeability, most likely mediated by the mechano-sensitive ion channel PIEZO1...
2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29610524/a-lever-like-transduction-pathway-for-long-distance-chemical-and-mechano-gating-of-the-mechanosensitive-piezo1-channel
#11
Yanfeng Wang, Shaopeng Chi, Huifang Guo, Guang Li, Li Wang, Qiancheng Zhao, Yu Rao, Liansuo Zu, Wei He, Bailong Xiao
Piezo1 represents a prototype of eukaryotic mechanotransduction channels. The full-length 2547-residue mouse Piezo1 possesses a unique 38-transmembrane-helix (TM) topology and is organized into a three-bladed, propeller-shaped architecture, comprising a central ion-conducting pore, three peripheral blade-like structures, and three 90-Å-long intracellular beam-resembling structures that bridge the blades to the pore. However, how mechanical force and chemicals activate the gigantic Piezo1 machinery remains elusive...
April 3, 2018: Nature Communications
https://www.readbyqxmd.com/read/29576450/common-piezo1-allele-in-african-populations-causes-rbc-dehydration-and-attenuates-plasmodium-infection
#12
Shang Ma, Stuart Cahalan, Gregory LaMonte, Nathan D Grubaugh, Weizheng Zeng, Swetha E Murthy, Emma Paytas, Ramya Gamini, Viktor Lukacs, Tess Whitwam, Meaghan Loud, Rakhee Lohia, Laurence Berry, Shahid M Khan, Chris J Janse, Michael Bandell, Christian Schmedt, Kai Wengelnik, Andrew I Su, Eric Honore, Elizabeth A Winzeler, Kristian G Andersen, Ardem Patapoutian
Hereditary xerocytosis is thought to be a rare genetic condition characterized by red blood cell (RBC) dehydration with mild hemolysis. RBC dehydration is linked to reduced Plasmodium infection in vitro; however, the role of RBC dehydration in protection against malaria in vivo is unknown. Most cases of hereditary xerocytosis are associated with gain-of-function mutations in PIEZO1, a mechanically activated ion channel. We engineered a mouse model of hereditary xerocytosis and show that Plasmodium infection fails to cause experimental cerebral malaria in these mice due to the action of Piezo1 in RBCs and in T cells...
March 8, 2018: Cell
https://www.readbyqxmd.com/read/29545531/voltage-gating-of-mechanosensitive-piezo-channels
#13
Mirko Moroni, M Rocio Servin-Vences, Raluca Fleischer, Oscar Sánchez-Carranza, Gary R Lewin
Mechanosensitive PIEZO ion channels are evolutionarily conserved proteins whose presence is critical for normal physiology in multicellular organisms. Here we show that, in addition to mechanical stimuli, PIEZO channels are also powerfully modulated by voltage and can even switch to a purely voltage-gated mode. Mutations that cause human diseases, such as xerocytosis, profoundly shift voltage sensitivity of PIEZO1 channels toward the resting membrane potential and strongly promote voltage gating. Voltage modulation may be explained by the presence of an inactivation gate in the pore, the opening of which is promoted by outward permeation...
March 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29525457/activation-of-piezo1-but-not-na-v-1-2-channels-by-ultrasound-at-43-mhz
#14
Martin Loynaz Prieto, Kamyar Firouzi, Butrus T Khuri-Yakub, Merritt Maduke
Ultrasound (US) can modulate the electrical activity of the excitable tissues, but the mechanisms underlying this effect are not understood at the molecular level or in terms of the physical modality through which US exerts its effects. Here, we report an experimental system that allows for stable patch-clamp recording in the presence of US at 43 MHz, a frequency known to stimulate neural activity. We describe the effects of US on two ion channels proposed to be involved in the response of excitable cells to US: the mechanosensitive Piezo1 channel and the voltage-gated sodium channel NaV 1...
June 2018: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/29521261/myotubularin-related-protein-2-and-its-phospholipid-substrate-pip-2-control-piezo2-mediated-mechanotransduction-in-peripheral-sensory-neurons
#15
Pratibha Narayanan, Meike Hütte, Galina Kudryasheva, Francisco J Taberner, Stefan G Lechner, Florian Rehfeldt, David Gomez-Varela, Manuela Schmidt
Piezo2 ion channels are critical determinants of the sense of light touch in vertebrates. Yet, their regulation is only incompletely understood. We recently identified myotubularin related protein-2 (Mtmr2), a phosphoinositide (PI) phosphatase, in the native Piezo2 interactome of murine dorsal root ganglia (DRG). Here, we demonstrate that Mtmr2 attenuates Piezo2-mediated rapidly adapting mechanically activated (RA-MA) currents. Interestingly, heterologous Piezo1 and other known MA current subtypes in DRG appeared largely unaffected by Mtmr2...
March 9, 2018: ELife
https://www.readbyqxmd.com/read/29498036/yoda1-analogue-dooku1-which-antagonizes-yoda1-evoked-activation-of-piezo1-and-aortic-relaxation
#16
Elizabeth L Evans, Kevin Cuthbertson, Naima Endesh, Baptiste Rode, Nicola M Blythe, Adam J Hyman, Sally J Hall, Hannah J Gaunt, Melanie J Ludlow, Richard Foster, David J Beech
BACKGROUND AND PURPOSE: The mechanosensitive Piezo1 channel has important roles in vascular physiology and disease. Yoda1 is a small-molecule agonist, but the pharmacology of these channels is otherwise limited. EXPERIMENTAL APPROACH: Yoda1 analogues were generated by synthetic chemistry. Intracellular Ca2+ and Tl+ measurements were made in HEK 293 or CHO cell lines overexpressing channel subunits and in HUVECs, which natively express Piezo1. Isometric tension recordings were made from rings of mouse thoracic aorta...
May 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29471041/mechanically-activated-ion-channels
#17
Boris Martinac, Kate Poole
The ability of cells to sense and respond to their physical environment is fundamental to a broad spectrum of biological processes. Cells express an array of force sensors that can transduce mechanical inputs into biochemical signals, including the mechanically activated ion channels PIEZO1, PIEZO2 and TRPV4. The identification and characterisation of mechanically activated ion channels has proven challenging, as has identifying their mode of activation and regulation in vivo. However, the diverse channelopathies associated with the known channels highlights their physiological importance...
April 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29469092/structure-and-mechanogating-mechanism-of-the-piezo1-channel
#18
Qiancheng Zhao, Heng Zhou, Shaopeng Chi, Yanfeng Wang, Jianhua Wang, Jie Geng, Kun Wu, Wenhao Liu, Tingxin Zhang, Meng-Qiu Dong, Jiawei Wang, Xueming Li, Bailong Xiao
The mechanosensitive Piezo channels function as key eukaryotic mechanotransducers. However, their structures and mechanogating mechanisms remain unknown. Here we determine the three-bladed, propeller-like electron cryo-microscopy structure of mouse Piezo1 and functionally reveal its mechanotransduction components. Despite the lack of sequence repetition, we identify nine repetitive units consisting of four transmembrane helices each-which we term transmembrane helical units (THUs)-which assemble into a highly curved blade-like structure...
February 22, 2018: Nature
https://www.readbyqxmd.com/read/29428723/yoda1-induced-phosphorylation-of-akt-and-erk1-2-does-not-require-piezo1-activation
#19
Nathaniel G Dela Paz, John A Frangos
Piezo1 is a mechanosensitive cation channel that is activated by shear stress in endothelial cells (ECs). It has been shown to mediate shear-induced EC responses, including increased calcium influx, and vascular functions, such as vascular tone and blood pressure. Yoda1, a selective Piezo1 activator, has been shown to mimic shear-induced responses in ECs. Since shear-induced calcium influx causes Akt and ERK1/2 activation in ECs, we examined the effects of Yoda1 and the role of Piezo1 on their activation. Here, we show that Yoda1 robustly activates Akt and ERK1/2 in ECs...
February 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29396846/multi-gene-panel-testing-improves-diagnosis-and-management-of-patients-with-hereditary-anemias
#20
Roberta Russo, Immacolata Andolfo, Francesco Manna, Antonella Gambale, Roberta Marra, Barbara Eleni Rosato, Paola Caforio, Valeria Pinto, Piero Pignataro, Kottayam Radhakrishnan, Sule Unal, Giovanna Tomaiuolo, Gian Luca Forni, Achille Iolascon
Mutations in more than 70 genes cause hereditary anemias (HA), a highly heterogeneous group of rare/low frequency disorders in which we included: hyporegenerative anemias, as congenital dyserythropoietic anemia (CDA) and Diamond-Blackfan anemia; hemolytic anemias due to erythrocyte membrane defects, as hereditary spherocytosis and stomatocytosis; hemolytic anemias due to enzymatic defects. The study describes the diagnostic workflow for HA, based on the development of two consecutive versions of a targeted-NGS panel, including 34 and 71 genes, respectively...
May 2018: American Journal of Hematology
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