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https://www.readbyqxmd.com/read/28330332/response-surface-methodology-based-optimization-of-production-media-and-purification-of-%C3%AE-galactosidase-in-solid-state-fermentation-by-fusarium-moniliforme-ncim-1099
#1
Sanjivani B Gajdhane, Prashant K Bhagwat, Padma B Dandge
Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett-Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone and FeSO4·7H2O were identified as significant medium components using PB design. Further CCRD optimized medium components as wheat bran; 4.62 μg, peptone; 315.42 μg, FeSO4·7H2O; 9.04 μg. RSM methodological optimization increased the enzyme production from 13...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330316/phytotoxicity-and-oxidative-stress-perspective-of-two-selected-nanoparticles-in-brassica-juncea
#2
Sunita Rao, Gyan Singh Shekhawat
This study elaborates the consequences of oxidative stress caused by copper oxide (CuO) and titanium dioxide (TiO2) nanoparticles (NPs) in Brassica juncea. Effect of these two NPs on plant physiology, reactive oxygen scavenging enzyme system (ascorbate peroxidase, catalase, superoxide dismutase), proline content and lipid peroxidation has been estimated in leaves as well as root tissues. Bioaccumulation of NPs has also been evaluated in the current study and the interrelated cascade of the enzymatic system with H2O2 production was identified...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330252/organic-solvent-tolerant-metallo-protease-of-novel-isolate-serratia-marcescens-ppb-26-production-and-characterization
#3
Shikha Thakur, Nirmal Kant Sharma, Neerja Thakur, Savitri, Tek Chand Bhalla
Proteases are a class of enzymes that catalyze hydrolysis of peptide bonds of proteins. In this study, 221 proteolytic bacterial isolates were obtained by enrichment culture method from soils of various regions of Himachal Pradesh, India. From these a hyper producer of protease was screened and identified by morphological and physiological testing and by 16S rDNA sequence as Serratia marcescens PPB-26. Statistical optimization of physiochemical parameters enhanced the protease production by 75 %. Protease of S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330247/production-of-glucosinolates-phenolic-compounds-and-associated-gene-expression-profiles-of-hairy-root-cultures-in-turnip-brassica-rapa-ssp-rapa
#4
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR. GSLs levels (glucoallysin, glucobrassicanapin, gluconasturtiin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and 4-hydroxyglucobrassicin) and their gene expression levels (BrMYB28, BrMYB29, BrMYB34, BrMYB51, BrMYB122, CYP79 and CYP83) significantly increased in hairy roots compared with that in non-transformed roots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330244/elucidation-of-impact-of-heavy-metal-pollution-on-soil-bacterial-growth-and-extracellular-polymeric-substances-flexibility
#5
Muniswamy David, Paidi Murali Krishna, Jeybalan Sangeetha
Metal bioaccessibility is an alarming issue in croplands of mining sites due to overloading of toxic metals. Hence, the present study is aimed to determine the overloading of toxic metal in croplands across the Tawag village, Hutti, Raichur, India. Correspondingly, to identify the soil bacterial growth, physiological oxidative stress enzyme activity and surface macromolecular functional group evolution were analysed in and around the toxic metal contaminated sites through FT-IR and FT-Raman spectrometry. The evaluated results attribute that the study area is heavily polluted with the toxic metals such as arsenic, cadmium, chromium, lead and zinc...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330182/efficient-single-step-chromatographic-purification-of-recombinant-human-antithrombin-rhat-from-saccharomyces-cerevisiae
#6
Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda
Antithrombin (AT) is a glycoprotein that inactivates the several physiological target enzymes of coagulation system. The effect of purification strategies plays a crucial role in getting maximum recovery of yield, purity and biological activity of recombinant human antithrombin (rhAT). In the present work, the task of purifying rhAT from Saccharomyces cerevisiae BY4741 has been carried out using two different approaches such as cross flow filtration (CFF) system and chromatography methods. In the first approach, the protein was concentrated and partially purified through CFF to achieve maximum recovery yield and purity of 87 and 94 %, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330141/evaluation-of-biochemical-markers-during-somatic-embryogenesis-in-silybum-marianum-l
#7
Bilal Haider Abbasi, Huma Ali, Buhara Yücesan, Sabahat Saeed, Khalid Rehman, Mubarak Ali Khan
In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic embryogenesis. Highest callus induction frequency (76 ± 4.8 %) was recorded when petiole explants of in vitro derived plantlets were cultured on B5 medium supplemented with 1...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330133/bioactive-metabolites-produced-by-streptomyces-cheonanensis-vuk-a-from-coringa-mangrove-sediments-isolation-structure-elucidation-and-bioactivity
#8
Ushakiranmayi Mangamuri, Vijayalakshmi Muvva, Sudhakar Poda, Krishna Naragani, Rajesh Kumar Munaganti, Bhujangarao Chitturi, Venkateswarlu Yenamandra
The strain VUK-A was isolated from a sediment sample of the Coringa mangrove ecosystem was identified as Streptomyces cheonanensis based on morphological, physiological, biochemical and molecular properties. Chemical investigation of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of two bioactive compounds, namely 2-Methyl butyl propyl phthalate (1) and Diethyl phthalate (2) using column chromatography. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including (1)H NMR and (13)C NMR spectroscopies, FTIR and EIMS...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330124/somaclonal-variations-and-their-applications-in-horticultural-crops-improvement
#9
REVIEW
Hare Krishna, Mahdi Alizadeh, Dhurendra Singh, Udayvir Singh, Nitesh Chauhan, Maliheh Eftekhari, Radha Kishan Sadh
The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330073/cladosporium-cladosporioides-from-the-perspectives-of-medical-and-biotechnological-approaches
#10
REVIEW
Manaf AlMatar, Essam A Makky
Fungi are important natural product sources that have enormous potential for the production of novel compounds for use in pharmacology, agricultural applications and industry. Compared with other natural sources such as plants, fungi are highly diverse but understudied. However, research on Cladosporium cladosporioides revealed the existence of bioactive products such as p-methylbenzoic acid, ergosterol peroxide (EP) and calphostin C as well as enzymes including pectin methylesterase (PME), polygalacturonase (PG) and chlorpyrifos hydrolase...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329914/ginsenoside-rg3-restores-hepatitis-c-virus-induced-aberrant-mitochondrial-dynamics-and-inhibits-virus-propagation
#11
Seong-Jun Kim, Jae Young Jang, Eun-Jung Kim, Eun Kyung Cho, Dae Gyun Ahn, Chonsaeng Kim, Han Seul Park, Soung Won Jeong, Sae Hwan Lee, Sang Gyune Kim, Young Seok Kim, Hong Soo Kim, Boo Sung Kim, Ji-Hyung Lee, Aleem Siddiqui
Hepatitis C virus (HCV) alters mitochondrial dynamics associated with persistent viral infection and suppression of innate immunity. Mitochondrial dysfunction is also a pathologic feature of direct-acting antiviral (DAA) treatment. Despite the high efficacy of DAAs, their treatment of patients with chronic hepatitis C in interferon-sparing regimens occasionally produces undesirable side effects such as fatigue, migraine and other conditions, which may be linked to mitochondrial dysfunction. Here we show that clinically prescribed DAAs, including Sofosbuvir, affect mitochondrial dynamics...
March 22, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28329750/optoelectronic-plethysmography-in-clinical-practice-and-research-a-review
#12
Carlo Massaroni, Elena Carraro, Andrea Vianello, Sandra Miccinilli, Michelangelo Morrone, Irisz K Levai, Emiliano Schena, Paola Saccomandi, Silvia Sterzi, John W Dickinson, Samantha Winter, Sergio Silvestri
BACKGROUND: Optoelectronic plethysmography (OEP) is a non-invasive motion capture method to measure chest wall movements and estimate lung volumes. OBJECTIVES: To provide an overview of the clinical findings and research applications of OEP in the assessment of breathing mechanics across populations of healthy and diseased individuals. METHODS: A bibliographic research was performed with the terms "opto-electronic plethysmography," "optoelectronic plethysmography," and "optoelectronic plethysmograph" in 50 digital library and bibliographic search databases resulting in the selection of 170 studies...
March 23, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/28329682/the-swi-snf-protein-pbrm1-restrains-vhl-loss-driven-clear-cell-renal-cell-carcinoma
#13
Amrita M Nargund, Can G Pham, Yiyu Dong, Patricia I Wang, Hatice U Osmangeyoglu, Yuchen Xie, Omer Aras, Song Han, Toshinao Oyama, Shugaku Takeda, Chelsea E Ray, Zhenghong Dong, Mathieu Berge, A Ari Hakimi, Sebastien Monette, Carl L Lekaye, Jason A Koutcher, Christina S Leslie, Chad J Creighton, Nils Weinhold, William Lee, Satish K Tickoo, Zhong Wang, Emily H Cheng, James J Hsieh
PBRM1 is the second most commonly mutated gene after VHL in clear cell renal cell carcinoma (ccRCC). However, the biological consequences of PBRM1 mutations for kidney tumorigenesis are unknown. Here, we find that kidney-specific deletion of Vhl and Pbrm1, but not either gene alone, results in bilateral, multifocal, transplantable clear cell kidney cancers. PBRM1 loss amplified the transcriptional outputs of HIF1 and STAT3 incurred by Vhl deficiency. Analysis of mouse and human ccRCC revealed convergence on mTOR activation, representing the third driver event after genetic inactivation of VHL and PBRM1...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28329679/mammalian-diaphanous-1-mediates-a-pathway-for-e-cadherin-to-stabilize-epithelial-barriers-through-junctional-contractility
#14
Bipul R Acharya, Selwin K Wu, Zi Zhao Lieu, Robert G Parton, Stephan W Grill, Alexander D Bershadsky, Guillermo A Gomez, Alpha S Yap
Formins are a diverse class of actin regulators that influence filament dynamics and organization. Several formins have been identified at epithelial adherens junctions, but their functional impact remains incompletely understood. Here, we tested the hypothesis that formins might affect epithelial interactions through junctional contractility. We focused on mDia1, which was recruited to the zonula adherens (ZA) of established Caco-2 monolayers in response to E-cadherin and RhoA. mDia1 was necessary for contractility at the ZA, measured by assays that include a FRET-based sensor that reports molecular-level tension across αE-catenin...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28329452/effect-of-prime-blood-storage-duration-on-clinical-outcome-after-pediatric-cardiac-surgery
#15
Arvind Kumar Bishnoi, Pankaj Garg, Kartik Patel, Parth Solanki, Jigar Surti, Atul Solanki, Komal Shah, Sanjay Patel
BACKGROUND: In this study, we tested the hypothesis that in pediatric patients undergoing cardiac surgery using cardiopulmonary bypass (CPB) with blood prime, the storage duration of the packed red blood cells (PRBCs) used in prime led to differences in postoperative complications and metabolic profiles of the patients. METHODS: For this prospective observational study we included 400 pediatric patients undergoing cardiac operations using CPB and requiring PRBCs prime...
March 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/28329433/understanding-maternal-postpartum-needs-a-descriptive-survey-of-current-maternal-health-services
#16
Mona Ma Almalik
OBJECTIVE: To assess mothers' learning needs and concerns after giving birth and to examine whether these needs were met at 6-8 weeks postpartum. BACKGROUND: Women experience many physiologic and psychological changes during postpartum period which is considered a vital transitional time. Exploring and meeting women's needs help woman to pass this period with little complications and enhance health care provider's ability to provide appropriate care following childbirth...
March 22, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28329355/international-recommendations-for-electrocardiographic-interpretation-in-athletes
#17
Sanjay Sharma, Jonathan A Drezner, Aaron Baggish, Michael Papadakis, Mathew G Wilson, Jordan M Prutkin, Andre La Gerche, Michael J Ackerman, Mats Borjesson, Jack C Salerno, Irfan M Asif, David S Owens, Eugene H Chung, Michael S Emery, Victor F Froelicher, Hein Heidbuchel, Carmen Adamuz, Chad A Asplund, Gordon Cohen, Kimberly G Harmon, Joseph C Marek, Silvana Molossi, Josef Niebauer, Hank F Pelto, Marco V Perez, Nathan R Riding, Tess Saarel, Christian M Schmied, David M Shipon, Ricardo Stein, Victoria L Vetter, Antonio Pelliccia, Domenico Corrado
Sudden cardiac death (SCD) is the leading cause of mortality in athletes during sport. A variety of mostly hereditary, structural, or electrical cardiac disorders are associated with SCD in young athletes, the majority of which can be identified or suggested by abnormalities on a resting 12-lead electrocardiogram (ECG). Whether used for diagnostic or screening purposes, physicians responsible for the cardiovascular care of athletes should be knowledgeable and competent in ECG interpretation in athletes. However, in most countries a shortage of physician expertise limits wider application of the ECG in the care of the athlete...
February 20, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28329240/effect-of-breathing-oxygen-enriched-air-on-exercise-performance-in-patients-with-precapillary-pulmonary-hypertension-randomized-sham-controlled-cross-over-trial
#18
Silvia Ulrich, Elisabeth D Hasler, Stéphanie Saxer, Michael Furian, Séverine Müller-Mottet, Stephan Keusch, Konrad E Bloch
Aims: The purpose of the current trial was to test the hypothesis that breathing oxygen-enriched air increases exercise performance of patients with pulmonary arterial or chronic thrombo-embolic pulmonary hypertension (PAH/CTEPH) and to investigate involved mechanisms. Methods and results: Twenty-two patients with PAH/CTEPH, eight women, means ± SD 61 ± 14 years, resting mPAP 35 ± 9mmHg, PaO2 ambient air >7.3 kPa, underwent four bicycle ergospirometries to exhaustion on different days, while breathing oxygen-enriched (FiO2 0...
March 11, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28329228/gender-differences-in-the-effects-of-cardiovascular-drugs
#19
J Tamargo, G Rosano, W Thomas, J Duarte, A Niessner, J C Kaski, C Cecconi, H Drexel, K Kjeldsen, G Savarese, C Torp-Pedersen, D Atar, B S Lewis, S Agewall
Although sex-specific differences in cardiovascular medicine are well-known, the exact influences of sex on the effect of cardiovascular drugs remain unclear. Women and men differ in body composition and physiology (hormonal influences during the menstrual cycle, menopause and pregnancy) and they present differences in drug pharmacokinetics (absorption, distribution, metabolism and excretion) and pharmacodynamics, so that is not rare that they may respond differently to cardiovascular drugs. Furthermore, women are also less often treated with evidence-based drugs thereby preventing optimization of therapeutics for women of all ages, experience more relevant adverse drug reactions than men, and remain under-represented in most clinical trials...
February 28, 2017: European Heart Journal. Cardiovascular Pharmacotherapy
https://www.readbyqxmd.com/read/28329170/life-span-extension-by-axenic-dietary-restriction-is-independent-of-the-mitochondrial-unfolded-protein-response-and-mitohormesis-in-caenorhabditis-elegans
#20
Huaihan Cai, Madina Rasulova, Lieselot Vandemeulebroucke, Lea Meagher, Caroline Vlaeminck, Ineke Dhondt, Bart P Braeckman
In Caenorhabditis elegans, a broad range of dietary restriction regimens extend life span to different degrees by separate or partially overlapping molecular pathways. One of these regimens, axenic dietary restriction, doubles the worm's life span but currently, almost nothing is known about the underlying molecular mechanism. Previous studies suggest that mitochondrial stress responses such as the mitochondrial unfolded protein response (UPRmt) or mitohormesis may play a vital role in axenic dietary restriction-induced longevity...
March 17, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
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