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https://www.readbyqxmd.com/read/28342932/changes-in-hba2-and-hbf-in-alpha-thalassemia-carriers-with-klf1-mutation
#1
Stefania Satta, Maria Elisabetta Paglietti, Maria Carla Sollaino, Susanna Barella, Paolo Moi, Maria Franca Desogus, Franca Rosa Demartis, Laura Manunza, Raffaella Origa
α-thalassemia carriers are common in Mediterranean regions, particularly in the Sardinian population. Their haematological phenotype is characterized by reduced MCV and/or MCH with normal or slightly reduced HbA2 levels and normal HbF. Krüppel-like factor 1 (KLF1) is a pleiotropic erythroid transcription factor that is essential for haematopoiesis. Mutations in the KLF1 gene trigger a series of benign human red blood phenotypes, such as an increase in HbA2 and HBF. Recently, it has been found that KLF1 mutations were a frequent cause of borderline HbA2 levels in a group of Sardinian subjects...
March 18, 2017: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/28340166/complete-blood-count-reference-ranges-from-a-rural-haitian-ambulatory-care-clinic
#2
Robert F Downey
Background: At rural healthcare centers in developing countries, it is difficult to recruit healthy individuals for reference range studies. So, in this study, I evaluated complete blood count (CBC) data to determine reference ranges for a rural Haitian population. Methods: A convenience sample of laboratory records was reviewed to obtain CBC results for 157 adult patients with normal screening examination results. Central 95% reference ranges were determined for male and female subjects...
March 10, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28336814/airway-smooth-muscle-dysfunction-in-pompe-gaa-mice
#3
Allison M Keeler, Donghai Liu, Marina Zieger, Lang Xiong, Jeffrey Salemi, Karl Bellve, Barry J Byrne, David D Fuller, Ronghua ZhuGe, Mai K ElMallah
Pompe disease is an autosomal recessive disorder caused by a deficiency of acid alpha-glucosidase (GAA) - an enzyme responsible for hydrolyzing lysosomal glycogen. Deficiency of GAA leads to systemic glycogen accumulation in the lysosomes of skeletal muscle, motor neurons and smooth muscle. Skeletal muscle and motor neuron pathology are known to contribute to respiratory insufficiency in Pompe disease, but the role of airway pathology has not been evaluated. Here we propose that GAA enzyme deficiency disrupts the function of the trachea and bronchi, and this lower airway pathology contributes to respiratory insufficiency in Pompe disease...
March 23, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28322083/diurnal-variation-of-the-melanin-concentrating-hormone-level-in-the-hypothalamus
#4
Balázs Gerics, Ferenc Szalay, Péter Sótonyi, Veronika Jancsik
Melanin-concentrating hormone (MCH), the neuropeptide produced mainly in the hypothalamus, plays an operative role in regulating food intake and the sleep/wake cycle. Considering that these physiological functions pursue diurnal variations, we checked whether the total hypothalamic MCH level depends on the time of the day. The aggregated MCH peptide content of the whole MCH neuron population was significantly higher at the end of the sleeping period (lights on), than at the end of the active period (lights off)...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28320934/allele-specific-non-cg-dna-methylation-marks-domains-of-active-chromatin-in-female-mouse-brain
#5
Christopher L Keown, Joel B Berletch, Rosa Castanon, Joseph R Nery, Christine M Disteche, Joseph R Ecker, Eran A Mukamel
DNA methylation at gene promoters in a CG context is associated with transcriptional repression, including at genes silenced on the inactive X chromosome in females. Non-CG methylation (mCH) is a distinct feature of the neuronal epigenome that is differentially distributed between males and females on the X chromosome. However, little is known about differences in mCH on the active (Xa) and inactive (Xi) X chromosomes because stochastic X-chromosome inactivation (XCI) confounds allele-specific epigenomic profiling...
March 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28318025/maternal-chronic-hypoxia-increases-expression-of-genes-regulating-lung-liquid-movement-and-surfactant-maturation-in-male-fetuses-in-late-gestation
#6
Erin V McGillick, Sandra Orgeig, Beth J Allison, Kirsty L Brain, Youguo Niu, Nozomi Itani, Katie L Skeffington, Andrew D Kane, Emilio A Herrera, Dino A Giussani, Janna L Morrison
Chronic fetal hypoxaemia is a common pregnancy complication that may arise from maternal, placental and/or fetal factors. Respiratory outcome of the infant at birth likely depends on the duration, timing and severity of the hypoxaemic insult. We have isolated the effect of maternal chronic hypoxia (MCH) for a month in late gestation on fetal lung development. Pregnant ewes were exposed to normoxia (21% O2 ) or hypoxia (10% O2 ) from 105-138 d gestation (term ∼145 d). At 138 d, gene expression in fetal lung tissue was determined by qRT-PCR...
March 20, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28301822/elevated-lead-levels-and-changes-in-blood-morphology-and-erythrocyte-cr1-in-preschool-children-from-an-e-waste-area
#7
Yifeng Dai, Xia Huo, Yu Zhang, Tian Yang, Minghui Li, Xijin Xu
Improper dismantling and combustion of electronic waste (e-waste) may release persistent organic pollutants and heavy metals that possess potential risk for human health. Lead (Pb) is carried through the circulatory system by erythrocytes and is known to alter the functions of hematopoietic and immune systems. The aim of the study was to investigate the effect of Pb exposure on blood morphology and erythrocyte complement receptor 1 (CR1) levels as related to immunologic function in preschool children. We recruited 484 preschool children, 2- to 6-years of age, among whom 332 children were from Guiyu, a typical and primitive e-waste processing area, and 152 children from Haojiang (reference area)...
March 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28300612/characterization-of-melanin-concentrating-hormone-mch-and-its-receptor-in-chickens-tissue-expression-functional-analysis-and-fasting-induced-up-regulation-of-hypothalamic-mch-expression
#8
Lin Cui, Chan Lv, Jiannan Zhang, Chunheng Mo, Dongliang Lin, Juan Li, Yajun Wang
Melanin-concentrating hormone (MCH) is a neuropeptide expressed in the brain and exerts its actions through interaction with the two known G protein-coupled receptors, namely melanin-concentrating hormone receptor 1 and 2 (MCHR1 and MCHR2) in mammals. However, the information regarding the expression and functionality of MCH and MCHR(s) remains largely unknown in birds. In this study, using RT-PCR and RACE PCR, we amplified and cloned a MCHR1-like receptor, which is named cMCHR4 according to its evolutionary origin, and a MCHR2 from chicken brain...
March 11, 2017: Gene
https://www.readbyqxmd.com/read/28294122/aptamer-based-field-effect-biosensor-for-tenofovir-detection
#9
N Aliakbarinodehi, P Jolly, N Bhalla, A Miodek, G De Micheli, P Estrela, S Carrara
During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28288662/a-mutant-tat-protein-inhibits-infection-of-human-cells-by-strains-from-diverse-hiv-1-subtypes
#10
Lina Rustanti, Hongping Jin, Mary Lor, Min Hsuan Lin, Daniel J Rawle, David Harrich
BACKGROUND: Nullbasic is a mutant HIV-1 Tat protein that inhibits HIV-1 replication via three independent mechanisms that disrupts 1) reverse transcription of the viral RNA genome into a DNA copy, 2) HIV-1 Rev protein function required for viral mRNA transport from the nucleus to the cytoplasm and 3) HIV-1 mRNA transcription by RNA Polymerase II. The Nullbasic protein is derived from the subtype B strain HIV-1BH10 and has only been tested against other HIV-1 subtype B strains. However, subtype B strains only account for ~10% of HIV-1 infections globally and HIV-1 Tat sequences vary between subtypes especially for subtype C, which is responsible for ~50% HIV-1 infection worldwide...
March 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28287537/visualization-of-cell-cycle-variations-and-determination-of-nucleation-in-postnatal-cardiomyocytes
#11
Alexandra Raulf, Nadine Voeltz, Daniel Korzus, Bernd K Fleischmann, Michael Hesse
Cardiomyocytes are prone to variations of the cell cycle, such as endoreduplication (continuing rounds of DNA synthesis without karyokinesis and cytokinesis) and acytokinetic mitosis (karyokinesis but no cytokinesis). Such atypical cell cycle variations result in polyploid and multinucleated cells rather than in cell division. Therefore, to determine cardiac turnover and regeneration, it is of crucial importance to correctly identify cardiomyocyte nuclei, the number of nuclei per cell, and their cell cycle status...
February 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28275474/effects-of-leukotriene-d4-nasal-challenge-on-bronchial-responsiveness-and-inflammation-in-asthmatic-patients-with-allergic-rhinitis
#12
Zheng Zhu, Yanqing Xie, Weijie Guan, Yi Gao, Rongquan Huang, Shu Xia, Wenhua Jian, Zhiyu Liang, Jinping Zheng
BACKGROUND: In asthmatic patients with allergic rhinitis (AR), increased cysteinyl leukotrienes (CysLTs) production in the secretion of nasal mucosa has been associated with greater bronchial hyperresponsiveness (BHR) after nasal allergen challenge. However, the role of CysLTs in eliciting BHR after nasal allergen challenge has not been evaluated. The aim of this study is to evaluate the effect of LTD4 nasal challenge on BHR and inflammation in asthmatic patients with AR. METHODS: In this self-controlled study, fifteen eligible consecutively recruited subjects underwent methacholine (Mch) bronchial provocation test before and 30 minutes after LTD4 nasal provocation test...
February 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28212941/injections-of-the-of-the-%C3%AE-1-adrenoceptor-antagonist-prazosin-into-the-median-raphe-nucleus-increase-food-intake-and-fos-expression-in-orexin-neurons-of-free-feeding-rats
#13
Eduardo Simão da Silva, Rafael Appel Flores, Anderson Savaris Ribas, Ana Paula Taschetto, Moacir Serralvo Faria, Leandro Bueno Lima, Martin Metzger, José Donato, Marta Aparecida Paschoalini
Previously, we showed that the blockade of α1-adrenoreceptors in the median raphe nucleus (MnR) increased food intake in free-feeding rats, indicating that adrenergic mechanisms in the MnR participate in the regulation of food intake. However, the impact of such a pharmacological manipulation on other neural circuits related to food intake remains unknown. In the current study, we sought to identify forebrain regions which are responsive to α1-adrenergic receptor blockade and presumably involved in the modulation of the feeding response...
May 1, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28212192/genetic-control-of-erythropoiesis
#14
Laxminath Tumburu, Swee Lay Thein
PURPOSE OF REVIEW: The discovery of several genetic variants associated with erythroid traits and subsequent elucidation of their functional mechanisms are exemplars of the power of the new genetic and genomic technology. The present review highlights findings from recent genetic studies related to the control of erythropoiesis and dyserythropoiesis, and fetal hemoglobin, an erythroid-related trait. RECENT FINDINGS: Identification of the genetic modulators of erythropoiesis involved two approaches: genome-wide association studies (GWASs) using single nucleotide polymorphism (SNP) arrays that revealed the common genetic variants associated with erythroid phenotypes (hemoglobin, red cell count, MCV, MCH) and fetal hemoglobin; and massive parallel sequencing such as whole genome sequencing (WGS) and whole exome sequencing (WES) that led to the discovery of the rarer variants (GFI1B, SBDS, RPS19, PKLR, EPO, EPOR, KLF1, GATA1)...
February 15, 2017: Current Opinion in Hematology
https://www.readbyqxmd.com/read/28202441/effect-of-hypohydration-on-postsynaptic-cutaneous-vasodilation-and-sweating-in-healthy-men
#15
Matthew A Tucker, Ashley Six, Nicole E Moyen, Alf Z Satterfield, Matthew S Ganio
Hypohydration decreases cutaneous vasodilation and sweating during heat stress, but it is unknown if these decrements are from postsynaptic (i.e. sweat gland/blood vessel) alterations. PURPOSE: To determine if hypohydration affects postsynaptic cutaneous vasodilation and sweating responses. METHODS: 12 healthy men participated in euhydrated (EU) and hypohydrated (HY) trials, with hypohydration induced via fluid restriction and passive heat-stress. Changes in cutaneous vascular conductance (CVC; %max) in response to incremental intradermal infusion of the endothelium-independent vasodilator sodium nitroprusside (SNP) and the endothelium-dependent vasodilator methacholine chloride (MCh) were assessed by laser Doppler flowmetry...
February 15, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28187897/the-application-of-a-decision-tree-to-establish-the-parameters-associated-with-hypertension
#16
Maryam Tayefi, Habibollah Esmaeili, Maryam Saberi Karimian, Alireza Amirabadi Zadeh, Mahmoud Ebrahimi, Mohammad Safarian, Mohsen Nematy, Seyed Mohammad Reza Parizadeh, Gordon A Ferns, Majid Ghayour-Mobarhan
INTRODUCTION: Hypertension is an important risk factor for cardiovascular disease (CVD). The goal of this study was to establish the factors associated with hypertension by using a decision-tree algorithm as a supervised classification method of data mining. METHODS: Data from a cross-sectional study were used in this study. A total of 9078 subjects who met the inclusion criteria were recruited. 70% of these subjects (6358 cases) were randomly allocated to the training dataset for the constructing of the decision-tree...
February 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28185007/the-rostromedial-zona-incerta-is-involved-in-attentional-processes-while-adjacent-lha-responds-to-arousal-c-fos-and-anatomical-evidence
#17
S Chometton, K Charrière, L Bayer, C Houdayer, G Franchi, F Poncet, D Fellmann, P Y Risold
Neurons producing melanin-concentrating hormone (MCH) are located in the tuberal lateral hypothalamus (LHA) and in the rostromedial part of the zona incerta (ZI). This distribution suggests that rostromedial ZI shares some common features with the LHA. However, its functions with regard to arousal or feeding, which are often associated with the LHA, have not been thoroughly investigated. This study analyses the responses in the tuberal LHA and adjacent rostromedial ZI after experiments related to arousal, exploration, food teasing and ingestive behavior...
February 9, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28182974/diamond-based-electrochemical-aptasensor-realizing-a-femtomolar-detection-limit-of-bisphenol-a
#18
Yibo Ma, Junsong Liu, Hongdong Li
In this study, we designed and fabricated an electrochemical impedance aptasensor based on Au nanoparticles (Au-NPs) coated boron-doped diamond (BDD) modified with aptamers, and 6-mercapto-1-hexanol (MCH) for the detection of bisphenol A (BPA). The constructed BPA aptasensor exhibits good linearity from 1.0×10(-14) to 1.0×10(-9)molL(-1). The detection limitation of 7.2×10(-15)molL(-1) was achieved, which can be attributed to the synergistic effect of combining BDD with Au-NPs, aptamers, and MCH. The examine results of BPA traces in Tris-HCl buffer and in milk, UV spectra of aptamer/BPA and interference test revealed that the novel aptasensors are of high sensitivity, specificity, stability and repeatability, which could be promising in practical applications...
June 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28182020/effect-of-ayurveda-medications-kas%C3%A4-sa-bhasma-and-dh%C3%A4-tr%C3%A4-avaleha-on-iron-deficiency-anaemia-a-randomized-controlled-study
#19
Basavaraj Ramappa Tubaki, Jyoti Mahadev Benni, Niranjan Rao, Uchangi Nagaraja Rao Prasad
BACKGROUND: This paper explores the role that Ayurveda can play in the management of Iron Deficiency Anaemia, a major nutritional deficiency disorder affecting people across the globe. METHODOLOGY: Forty (40) patients suffering from Iron deficiency anaemia as per WHO guidelines, between the age group of 20 to 60 yrs of either sex participated in the study. Study was a randomized, controlled, open label clinical study. Patients were randomly divided into two groups: Group D (n = 20) received Dhārī avaleha 10 g twice a day after food...
July 2016: Ancient Science of Life
https://www.readbyqxmd.com/read/28169460/alterations-of-the-hematologic-cells-in-synthetic-cannabinoid-users
#20
Derya Guzel, Ahmet Bulent Yazici, Esra Yazici, Atila Erol
BACKGROUND: Functions, morphology, distributions, and index of the circulating cells are the most useful parameters that indicate various inflammatory and toxic conditions. The aim of this study was to investigate the clinical significance of these parameters in patients diagnosed with (synthetic) cannabis use disorder. METHODS: This study included a total of 40 patients in the study group (SG) with synthetic cannabis use; and 40 healthy individuals as the control group (CG)...
February 7, 2017: Journal of Clinical Laboratory Analysis
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