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https://www.readbyqxmd.com/read/28929959/-in-vitro-effect-of-vancomycin-and-daptomycin-on-biofilm-formation-of-coagulase-negative-staphylococci-strains
#1
Duygu Nilüfer Öcal, İştar Dolapçı, Zeynep Gençtürk, Alper Tekeli
Coagulase-negative staphylococci (CNS) are one of the primer agents of blood stream infections (BSI) and catheter-related bloodstream infections (CR-BSI) which are associated mostly with the usage of central venous catheters and, important causes of morbidity and mortality despite the usage of antibacterial and supportive treatment. It is important to determine the properties of these causative microorganisms in order to make appropriate treatment of such infections. The aims of our study were to evaluate the biofilm formation of coagulase negative staphylococci (CNS) which were causative agents of bloodstream (BSI) and catheter related bloodstream infections (CR-BSI), to determine the minimum inhibitory concentration (MIC) of planktonic forms and minimal biofilm eradication concentration (MBEC) of sessile forms for vancomycin and daptomycin and to evaluate the efficacy of these antibiotics in infections with biofilm-forming isolates in vitro...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28924586/effectiveness-and-safety-of-metallic-stent-for-ileocecal-obstructive-colon-cancer-a-report-of-4-cases
#2
Tatsuya Ishii, Kosuke Minaga, Satoshi Ogawa, Maiko Ikenouchi, Tomoe Yoshikawa, Takuji Akamatsu, Takeshi Seta, Shunji Urai, Yoshito Uenoyama, Yukitaka Yamashita
Background and study aims  Self-expandable metallic stents (SEMS) have been widely used for left-sided colorectal obstruction. Few studies on SEMS placement for right-sided colonic obstructions have been reported because stenting in the right colon is technically difficult, particularly in the ileocecal region. We present 4 cases of successful bridge-to-surgery stenting for ileocecal cancer. Using an endoscopic retrograde cholangiopancreatography catheter with a movable tip and a decompression tube was effective for stenting...
September 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28900004/network-of-microbial-and-antibiotic-interactions-drive-colonization-and-infection-with-multidrug-resistant-organisms
#3
Joyce Wang, Betsy Foxman, Lona Mody, Evan S Snitkin
The emergence and spread of multidrug-resistant organisms (MDROs) across global healthcare networks poses a serious threat to hospitalized individuals. Strategies to limit the emergence and spread of MDROs include oversight to decrease selective pressure for MDROs by promoting appropriate antibiotic use via antibiotic stewardship programs. However, restricting the use of one antibiotic often requires a compensatory increase in the use of other antibiotics, which in turn selects for the emergence of different MDRO species...
September 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28883011/rare-case-of-massive-bilateral-chylothorax
#4
Guramrinder Singh Thind, Dominika M Zoltowska, Yashwant Agrawal
We present a case of a 48-year-old woman who was referred from an outside hospital. There, she had initially underwent hysterectomy and left salpingo-oophorectomy for tubo-ovarian abscess. She later developed a colovaginal fistula and perforation of sigmoid colon and underwent Hartmann's procedure along with drainage of a left subphrenic abscess. Subsequently, she had to be intubated for acute respiratory failure and was transferred to our hospital. At our hospital, she was found to have massive bilateral pleural effusions...
September 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28876571/duodenectomy-managing-the-fistula
#5
M Govender, O Matsevych, F Ghoor, N Singh, C Molenaar
BACKGROUND: Duodenectomy is rarely indicated, however, in certain circumstances may have be performed. Enterocutaneous fistula (ECF) is the main cause of serious adverse outcomes. Its management remains challenging. METHOD: Two cases of emergency duodenectomy are presented. The management of ECF is described and discussed. RESULTS: Case 1: A 22-year-old male presented in septic shock with perforated duodenal ulcer, suffered two cardiac arrests before index surgery...
September 2017: South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie
https://www.readbyqxmd.com/read/28873268/a-novel-in-vitro-model-for-hematogenous-spreading-of-s-aureus-device-biofilms-demonstrating-clumping-dispersal-as-an-advantageous-dissemination-mechanism
#6
R B Grønnemose, K L Saederup, H J Kolmos, S W K Hansen, C A Asferg, K J Rasmussen, Y Palarasah, T E Andersen
Staphylococcus aureus is able to disseminate from vascular device biofilms to the blood and organs, resulting in life-threatening infections such as endocarditis. The mechanisms behind spreading are largely unknown, especially how the bacterium escapes immune effectors and antibiotics in the process. Using an in vitro catheter infection model, we studied S. aureus biofilm growth, late-stage dispersal, and reattachment to downstream endothelial cell layers. The ability of the released biofilm material to resist host response and disseminate in vivo was furthermore studied in whole blood- and phagocyte survival assays, and in a short-term murine infection model...
September 5, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28860709/%C3%AE-melanocyte-stimulating-hormone-modulates-the-central-acyl-ghrelin-induced-stimulation-of-feeding-gastrointestinal-motility-and-colonic-secretion
#7
Hsien-Hao Huang, Liang-Yu Chen, Ming-Luen Doong, Shi-Chuan Chang, Chih-Yen Chen
BACKGROUND: Acyl ghrelin-induced intake depends on hypothalamic neuropeptide Y and agouti-related protein (AgRP) neurotransmitters. Intracerebroventricular (ICV) injection of AgRP increases feeding through competitive antagonism at melanocortin receptors. ICV administration of α-melanocyte stimulating hormone (α-MSH), a natural antagonist of AgRP, may modulate the acyl ghrelin-induced orexigenic effect. OBJECTIVE: This study aimed to investigate the modulating effect of α-MSH on the central acyl ghrelin-induced food intake, gastrointestinal motility, and colonic secretion in rats...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28859352/new-approaches-to-prevent-healthcare-associated-infection
#8
Philippe Brouqui, Sophia Boudjema, Alberto Soto Aladro, Eric Chabrière, Olga Florea, Hoa Nguyen, Jean Charles Dufour
Healthcare-associated infection (HCAI) in hospitals mainly results from unsolved but well-identified causes such as hand hygiene, overuse of catheters, and to a lesser extent, the airborne transmission of infectious agents caused by the misuse of respiratory precautions. The aims of the Institut Hospitalo-Universitaire Méditerranée Infection are to develop new approaches to fight HCAIs. Among them, new technologies that allow for the traceability of care and good practices reminders have been developed concomitantly to an anthropological approach, facilitating acceptability by healthcare workers...
August 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28859349/from-expert-protocols-to-standardized-management-of-infectious-diseases
#9
Jean-Christophe Lagier, Camille Aubry, Marion Delord, Pierre Michelet, Hervé Tissot-Dupont, Matthieu Million, Philippe Brouqui, Didier Raoult, Philippe Parola
We report here 4 examples of management of infectious diseases (IDs) at the University Hospital Institute Méditerranée Infection in Marseille, France, to illustrate the value of expert protocols feeding standardized management of IDs. First, we describe our experience on Q fever and Tropheryma whipplei infection management based on in vitro data and clinical outcome. Second, we describe our management-based approach for the treatment of infective endocarditis, leading to a strong reduction of mortality rate...
August 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28844492/low-prevalence-of-colonization-with-multidrug-resistant-gram-negative-bacteria-in-long-term-care-facilities-in-graz-austria
#10
Eva Leitner, Elisabeth Zechner, Elisabeth Ullrich, Gernot Zarfel, Josefa Luxner, Christian Pux, Gerald Pichler, Walter Schippinger, Robert Krause, Ines Zollner-Schwetz
BACKGROUND: Residents in long-term care facilities (LTCFs) are increasingly found to be an important reservoir of multidrug-resistant gram-negative (MRGN) bacteria. AIMS: We aimed to determine colonization by MRGN bacteria over 6 months in LTCFs and geriatric wards in Graz, Austria, and to evaluate risk factors for such colonization. METHODS: During August 2015, we conducted a point-prevalence survey at LTCFs and geriatric wards of the Geriatric Health Centers of the City of Graz...
August 24, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28836247/-application-and-effect-evaluation-of-an-self-made-device-to-fix-liver-in-laparoscopic-radical-gastrectomy
#11
Xiaoyu Dong, Xiaoyu Chen, Yu Zhu, Zixuan Liu, Fengyue Jiang, Jun Luo, Jiang Yu, Guoxin Li, Liying Zhao
OBJECTIVE: To introduce the application and clinical effect of self-made liver fixing device in laparoscopic radical gastrectomy. METHODS: Clinical data of 469 patients underwent laparoscopic radical gastrectomy in Nanfang Hospital, Southern Medical University from March 2014 to January 2017 were analyzed retrospectively. In laparoscopic radical gastrectomy, self-made liver fixing device was used to expose surgical field covered by hepatic lobe in gastric lesser curvature and hepatic flexure of colon...
August 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28806303/does-a-combination-of-laparoscopic-approach-and-full-fast-track-multimodal-management-decrease-postoperative-morbidity-a-multicenter-randomized-controlled-trial
#12
Léon Maggiori, Eric Rullier, Jérémie H Lefevre, Jean-Marc Régimbeau, Stéphane Berdah, Mehdi Karoui, Jérome Loriau, Arnaud Alvès, Eric Vicaut, Yves Panis
OBJECTIVE: The aim of this study was to assess whether association of laparoscopic approach and full fast track multimodal (FFT) management can reduce postoperative morbidity after colorectal cancer surgery, as compared to laparoscopic approach with limited fast-track program (LFT). SUMMARY OF BACKGROUND DATA: Recent advances in colorectal cancer surgery are introduction of laparoscopy and FFT implementation. METHODS: Patients eligible for elective laparoscopic colorectal cancer surgery were randomized into 2 groups: FFT or LFT care (with only early oral intake and mobilization starting on Day 1)...
August 11, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28791879/management-of-colon-perforation-during-percutaneous-nephrolithotomy-12-years-of-experience-in-a-referral-center
#13
Robab- Maghsoudi, Massoud Etemadian, Amir Hossein Kashi, Kaveh Mehravaran
INTRODUCTION: The management protocol for colon perforation during percutaneous nephrolithotomy (PNL) is controversial due to the scarcity of reported cases and their management diversity. We present our management experience of colon perforation during PNL. MATERIALS AND METHODS: All PNL operations between April 2004 and September 2016 in our center (N=11376) were reviewed for the occurrence and management of colon perforation. We typically performed PNL with insertion of nephrostomy tube before mid 2007...
August 9, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28782707/esbl-producing-enterobacteriaceae-colonization-in-long-term-care-facilities-ltcfs-a-systematic-review-and-meta-analysis
#14
REVIEW
Myrto Eleni Flokas, Michail Alevizakos, Fadi Shehadeh, Nikolaos Andreatos, Eleftherios Mylonakis
OBJECTIVES: To estimate the colonization rate by extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among residents of Long Term Care facilities (LTCFs) and to identify pertinent risk factors. METHODS: A systematic search of PubMed and EMBASE databases for studies published up to May, 2016 that provided raw data for gastrointestinal colonization by ESBL-PE among LTCF residents. RESULTS: Twenty three studies reporting data on 9,775 screened subjects met our inclusion criteria...
August 3, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28777419/the-neutraclear%C3%A2-needleless-connector-is-equally-effective-against-catheter-colonization-compared-to-microclave%C3%A2
#15
María Guembe, María Jesús Pérez Granda, Raquel Cruces, Luis Alcalá, Emilio Bouza
INTRODUCTION: Neutral-valve closed-system connectors can reduce the frequency of catheter colonization. Commercially available closed system connectors need to be tested and compared with each other to assess how they protect against contamination. We aimed to compare, in vitro, the efficacy of connectors NeutraClear® and MicroClave® against contamination under conditions of daily clinical practice. METHODS: The model consisted of a set of 200 blood culture bottles (BCBs) with a cannula inserted (100 closed with NeutraClear® and 100 closed with MicroClave®) that were assessed in two experiments while instilling 1 mL of saline: manipulation based on the standard of care and manipulation using gloves impregnated with a 0...
July 29, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28752736/influence-of-skin-disinfection-prior-removal-of-perineural-catheter-on-bacterial-colonization-contamination-and-local-inflammation-a-prospective-randomized-study
#16
Stephan Blumenthal, Reinhard Zbinden, Sascha Mandic, Christoph A Rüst, José Aguirre, Alain Borgeat
BACKGROUND: There is a wide variation of perineural catheter (PNC) colonization rates in the literature. The impact of skin disinfection on PNC colonization and inflammation is not clear. The objective of this prospective, randomized clinical study was to investigate the influence of alcoholic skin disinfection before PNC removal on the detection of bacteria on PNC. METHODS: Two hundred patients receiving a PNC for orthopaedic surgery were random- ized to receive (with-group) or not (without-group) a skin disinfection with a sprayed alcoholic solution before removal of the PNC...
July 28, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/28750921/the-methodology-of-surveillance-for-antimicrobial-resistance-and-healthcare-associated-infections-in-europe-suspire-a-systematic-review-of-publicly-available-information
#17
REVIEW
María Núñez-Núñez, María Dolores Navarro, Virginia Palomo, Nithya Babu Rajendran, María Dolores Del Toro, Andreas Voss, Mike Sharland, Frangiscos Sifakis, Evelina Tacconelli, Jesús Rodríguez-Baño
OBJECTIVES: Surveillance is a key component of any control strategy for health-care associated infections (HAIs) and antimicrobial resistance (AMR), and public availability of methodological aspects is crucial for the interpretation of the data. We sought to systematically review publicly available information for HAIs and/or AMR surveillance systems organised by public institutions or scientific societies in European countries. METHODS: A systematic review of scientific and grey literature following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines was performed...
July 24, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28745020/distinct-roles-of-the-7-transmembrane-receptor-protein-rta3-in-regulating-the-asymmetric-distribution-of-phosphatidylcholine-across-the-plasma-membrane-and-biofilm-formation-in-candida-albicans
#18
Archita Srivastava, Shabnam Sircaik, Farha Husain, Edwina Thomas, Shivani Ror, Sumit Rastogi, Darakshan Alim, Priyanka Bapat, David R Andes, Clarissa J Nobile, Sneh L Panwar
Fungal pathogens like Candida albicans exhibit several survival mechanisms to evade attack by antifungals and colonize host tissues. Rta3, a member of the Rta1-like family of lipid-translocating exporters has a 7-transmembrane domain (7TMD) topology, similar to the G-protein-coupled receptors (GPCR) and is unique to the fungal kingdom. Our findings point towards a role for the plasma membrane localized Rta3 in providing tolerance to miltefosine, an analog of alkylphosphocholine, by maintaining mitochondrial energetics...
July 25, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28743533/should-non-bacteraemic-patients-with-a-colonized-catheter-receive-antimicrobial-therapy
#19
V De Egea, M Guembe, A Rodríguez-Borlado, M J Pérez-Granda, C Sánchez-Carrillo, E Bouza
OBJECTIVES: The impact of antimicrobial therapy on the outcomes of patients with colonized catheters and no bacteraemia has not been assessed. This study assessed whether targeted antibiotic therapy is related to a poor outcome in patients with positive cultures of blood drawn through a non-tunnelled central venous catheter (CVC) and without concomitant bacteraemia. METHODS: This was a retrospective study involving adult patients with positive blood cultures drawn through a CVC and negative peripheral vein blood cultures...
September 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28743387/polymerase-chain-reaction-based-detection-of-bacterial-16s-rrna-gene-in-the-cerebrospinal-fluid-in-the-diagnosis-of-bacterial-central-nervous-system-infection-in-the-course-of-external-cerebrospinal-fluid-drainage-comparison-with-standard-diagnostics-currently
#20
Piotr Dąbrowski, Jerzy Jurkiewicz, Zbigniew Czernicki, Waldemar Koszewski, Piotr Jasielski
BACKGROUND AND PURPOSE: External drainage of cerebrospinal fluid (CSF) is a commonly used neurosurgical procedure. Complications of the procedure comprise central nervous system (CNS) bacterial infections, the frequency of which is estimated at around 6-10%. Detection of these infections is ineffective in many cases. The aim of the study was to evaluate the usefulness of a polymerase chain reaction (PCR)-based detection of bacterial 16S rRNA gene (16S rDNA) in the CSF. MATERIAL AND METHODS: The study group consisted of 50 patients...
July 15, 2017: Neurologia i Neurochirurgia Polska
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