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https://www.readbyqxmd.com/read/28436457/fasciola-hepatica-glycoconjugates-immuneregulate-dendritic-cells-through-the-dendritic-cell-specific-intercellular-adhesion-molecule-3-grabbing-non-integrin-inducing-t-cell-anergy
#1
Ernesto Rodríguez, Hakan Kalay, Verónica Noya, Natalie Brossard, Cecilia Giacomini, Yvette van Kooyk, Juan J García-Vallejo, Teresa Freire
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is a C-type lectin receptor that recognizes glycans on a diverse range of pathogens, including parasites. The interaction of DC-SIGN with pathogens triggers specific signaling events that modulate DC-maturation and activity and regulate T-cell activation by DCs. In this work we evaluate whether F. hepatica glycans can immune modulate DCs via DC-SIGN. We demonstrate that DC-SIGN interacts with F. hepatica glycoconjugates through mannose and fucose residues...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28435817/molecular-and-morphometrical-characterization-of-fasciola-species-isolated-from-domestic-ruminants-in-ardabil-province-northwestern-iran
#2
Mojgan Aryaeipour, Arezoo Bozorgomid, Bahram Kazemi, Masoud Behnia, Hakim Azizi, Mohammad Bagher Rokni
BACKGROUND: We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. METHODS: Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms...
March 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28426420/disruption-of-egg-production-by-triclabendazole-resistant-fasciola-hepatica-following-treatment-with-a-commercial-preparation-of-myrrh-mirazid
#3
Mohamed M O Abdelaal, Gerard P Brennan, Robert E B Hanna, Ahmed Abdel-Aziz, Ian Fairweather
An in vitro study has been carried out to monitor changes to the female reproductive system in adult triclabendazole (TCBZ)-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh ("Mirazid"). Flukes were immersed for 6 h and 24 h in myrrh extract at a concentration of 200 µg/ml, then processed for histological and transmission electron microscope (TEM) examination of the uterus, Mehlis' gland, ovary and vitellaria. Egg production had become abnormal at 6 h post-treatment (pt), with the uterine lumen being filled with free vitelline cells and masses of shell protein material; few eggs were present...
June 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28410676/the-immunoregulatory-effects-of-co-infection-with-fasciola-hepatica-from-bovine-tuberculosis-to-johne-s-disease
#4
REVIEW
Amalia Naranjo Lucena, Laura Garza Cuartero, Grace Mulcahy, Annetta Zintl
Fasciola hepatica (liver fluke) is a parasite prevalent in much of the world that causes the economically-important disease of fasciolosis in livestock. The threat that this disease poses extends beyond its direct effects due to the parasite's immunomodulatory effects. Research at this laboratory is focusing on whether this immunoregulation can, in animals infected with liver fluke, exert a bystander effect on concurrent infections in the host. It has already been established that F. hepatica infection reduces cell mediated immune responses to Mycobacterium bovis in cattle, and that the interaction between the two pathogens can be detected on an epidemiological scale...
April 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28410387/mathematical-algorithm-for-the-automatic-recognition-of-intestinal-parasites
#5
Alicia Alva, Carla Cangalaya, Miguel Quiliano, Casey Krebs, Robert H Gilman, Patricia Sheen, Mirko Zimic
Parasitic infections are generally diagnosed by professionals trained to recognize the morphological characteristics of the eggs in microscopic images of fecal smears. However, this laboratory diagnosis requires medical specialists which are lacking in many of the areas where these infections are most prevalent. In response to this public health issue, we developed a software based on pattern recognition analysis from microscopi digital images of fecal smears, capable of automatically recognizing and diagnosing common human intestinal parasites...
2017: PloS One
https://www.readbyqxmd.com/read/28407808/nested-pcr-assay-for-detection-of-schistosoma-japonicum-infection-in-domestic-animals
#6
Xin Zhang, Chuan-Chuan He, Jin-Ming Liu, Hao Li, Ke Lu, Zhi-Qiang Fu, Chuan-Gang Zhu, Yi-Ping Liu, Lai-Bao Tong, De-Bao Zhou, Li Zha, Yang Hong, Ya-Mei Jin, Jiao-Jiao Lin
BACKGROUND: Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary. It was reported that polymerase chain reaction (PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity...
April 13, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28396295/molecular-characterization-of-fasciola-flukes-obtained-from-wild-sika-deer-and-domestic-cattle-in-hokkaido-japan
#7
Madoka Ichikawa-Seki, Tomoko Shiroma, Tatsuya Kariya, Ryo Nakao, Yuma Ohari, Kei Hayashi, Shinya Fukumoto
The number of wild sika deer (Cervus nippon yesoensis) continues to increase in Hokkaido Prefecture, Japan. The major concern for the livestock industry is the transmission of pathogens between sika deer and cattle. Fasciolosis is an important disease that can occur in both animals. The aim of this study was to examine the possible mutual transmission of this disease in Hokkaido Prefecture. A total of 105 Fasciola flukes were obtained from sika deer and 96 from domestic cattle. The Fasciola flukes in Japan are reported to possess no mature sperm...
April 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28385589/lymphocyte-responses-of-rats-vaccinated-with-cdna-encoding-a-phosphoglycerate-kinase-of-fasciola-hepatica-fhpgk-and-f-hepatica-infection
#8
Agnieszka Wesołowska, Anna Zawistowska-Deniziak, Luke J Norbury, Przemysław Wilkowski, Anna M Pyziel, Wojciech Zygner, Halina Wędrychowicz
Lymphocyte responses in the blood, peritoneal fluid and both mesenteric and hepatic lymph nodes of cDNA-FhPGK/pCMV vaccinated and/or Fasciola hepatica infected rats of both sexes were investigated to provide an insight into the immune responses that develop in different body compartments. The immune response that developed in cDNA-FhPGK/pCMV vaccinated females contributed to partial protection against F. hepatica infection (54% reduction in fluke recovery), while more liver flukes were found in the livers and bile ducts of cDNA-FhPGK/pCMV vaccinated male rats than in unvaccinated animals (increase of 13%)...
April 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28385539/th1-th2-balance-in-the-liver-and-hepatic-lymph-nodes-of-vaccinated-and-unvaccinated-sheep-during-acute-stages-of-infection-with-fasciola-hepatica
#9
I L Pacheco, N Abril, N Morales-Prieto, M J Bautista, R Zafra, A Escamilla, M T Ruiz, A Martínez-Moreno, J Pérez
The expression of IFNγ and IL4 was quantified using q-PCR in the liver and hepatic lymph nodes (HLN) of sheep during early stages of infection with Fasciola hepatica (1, 3, 9 and 18days post-infection, dpi). A group of animals (Group 1) were vaccinated with Fasciola hepatica recombinant cathepsin L1 (FhCL1) in montanide 70 VG prior to infection, a second group (group 2) was used as infected control and a third (group 3) was used as uninfected control. To study vaccine efficacy three additional groups were sacrificed 19 weeks post-infection (group 4 immunized with CL1, group 5 with the adjuvant and group 6 was used as infected control)...
March 25, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28365925/prevalence-economic-analysis-and-chemotherapeutic-control-of-small-ruminant-fasciolosis-in-the-sargodha-district-of-punjab-pakistan
#10
Mansoor Ahmad, Muhammad Nisar Khan, Muhammad Sohail Sajid, Ghulam Muhammad, Abdul Qudoos, Hafiz Muhammad Rizwan
This study describes the epidemiology, the economic significance of small ruminant fasciolosis in animals slaughtered in the abattoirs of the Sargodha district, Punjab, Pakistan between January and June 2012. In vivo fasciolicidal efficacy of commercially available compounds was examined using a randomised complete block design. Microscopically screened faecal samples revealed 40.51% positive animals for fasciolosis. The prevalent species included Fasciola hepatica (35.64%) and Fasciola gigantica (8.21%). Mixed infections were noted in 3...
March 31, 2017: Veterinaria Italiana
https://www.readbyqxmd.com/read/28362822/fasciola-hepatica-reinfection-potentiates-a-mixed-th1-th2-th17-treg-response-and-correlates-with-the-clinical-phenotypes-of-anemia
#11
M Adela Valero, Ignacio Perez-Crespo, Carlos Chillón-Marinas, Messaoud Khoubbane, Carla Quesada, Marta Reguera-Gomez, Santiago Mas-Coma, Manuel Fresno, Núria Gironès
BACKGROUND: Fascioliasis is a severe zoonotic disease of worldwide extension caused by liver flukes. In human fascioliasis hyperendemic areas, reinfection and chronicity are the norm and anemia is the main sign. Herein, the profile of the Th1/Th2/Th17/Treg expression levels is analyzed after reinfection, correlating them with their corresponding hematological biomarkers of morbidity. METHODOLOGY/PRINCIPAL FINDINGS: The experimental design reproduces the usual reinfection/chronicity conditions in human fascioliasis endemic areas and included Fasciola hepatica primo-infected Wistar rats (PI) and rats reinfected at 8 weeks (R8), and at 12 weeks (R12), and negative control rats...
2017: PloS One
https://www.readbyqxmd.com/read/28360908/fasciola-hepatica-immune-regulates-cd11c-cells-by-interacting-with-the-macrophage-gal-galnac-lectin
#12
Ernesto Rodríguez, Paula Carasi, Sofía Frigerio, Valeria da Costa, Sandra van Vliet, Verónica Noya, Natalie Brossard, Yvette van Kooyk, Juan J García-Vallejo, Teresa Freire
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28348084/delineating-distinct-heme-scavenging-and-binding-functions-of-domains-in-mf6p-hdm-proteins-from-parasitic-flatworms
#13
Victoria Martínez-Sernández, Mercedes Mezo, Marta González-Warleta, María J Perteguer, Teresa Gárate, Fernanda Romarís, Florencio M Ubeira
MF6p/FhHDM-1 is a small protein secreted by the parasitic flatworm (trematode) Fasciola hepatica and belonging to a broad family of heme-binding proteins (MF6p/HDMs). MF6p/HDMs are of interest for understanding heme homeostasis in trematodes and also as potential targets for the development of new flukicides. Moreover, interest in these molecules has also increased due to their immunomodulatory properties. Here we have extended our previous findings on the mechanism of MF6p/HDM-heme interactions and have mapped the protein regions required for heme binding and for other biological functions...
March 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28342671/apoptosis-of-peritoneal-leucocytes-during-early-stages-of-fasciola-hepatica-infections-in-sheep
#14
A Escamilla, R Pérez-Caballero, R Zafra, M J Bautista, I L Pacheco, M T Ruiz, M S Martínez-Cruz, A Martínez-Moreno, V Molina-Hernández, J Pérez
Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n=5) of sheep were used. Groups 2-5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18days post-infection (dpi), respectively...
March 18, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28333957/immune-response-of-rats-vaccinated-orally-with-various-plant-expressed-recombinant-cysteine-proteinase-constructs-when-challenged-with-fasciola-hepatica-metacercariae
#15
Malgorzata Kesik-Brodacka, Agnieszka Lipiec, Monika Kozak Ljunggren, Luiza Jedlina, Katarzyna Miedzinska, Magdalena Mikolajczak, Andrzej Plucienniczak, Andrzej B Legocki, Halina Wedrychowicz
BACKGROUND: Cysteine proteinases of Fasciola hepatica are important candidates for vaccine antigens because of their role in fluke biology and host-parasite relationships. In our previous experiments, we found that a recombinant cysteine proteinase cloned from adult F. hepatica (CPFhW) can protect rats against liver fluke infections when it is administered intramuscularly or intranasally in the form of cDNA. We also observed considerable protection upon challenge following mucosal vaccination with inclusion bodies containing recombinant CPFhW produced in Escherichia coli...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28327829/-western-blot-diagnostic-yield-for-simultaneous-antibody-detection-in-patients-with-human-cysticercosis-hydatidosis-and-human-fascioliasis
#16
Kelly Davelois, Hermes Escalante, César Jara
. To determine the diagnostic yield using western blotting to simultaneously detect antibodies in patients with human cysticercosis, hydatidosis, and human fascioliasis. Materials and methods . Cross-sectional study of diagnostic yield assessment. Excretory/secretory antigens were obtained from Taenia solium larvae, Echinococcus granulosus cysts, and the adult flukes of Fasciola hepática, which were then separated using the polyacrylamide gel electrophoresis technique, transferred, and attached to a nitrocellulose membrane to be probed with sera from the patient infected with the three parasites...
October 2016: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28316393/chronic-bovine-fasciolosis-associated-cholangiolithiasis-a-case-report
#17
Ananda K Javaregowda, Kavitha B Rani
Cholangiolithiasis is a rare condition in animals wherein the choleliths are formed in the biliary tracts causing chronic inflammation. In the current study, choleliths which were firm and friable concretions of approximately 3-4 cm in diameter were found in the bile ducts of the liver of an adult cattle infected with Fasciola gigantica. Two such irregularly shaped concretions were encountered in the two biliary tracts leading to common bile duct in the liver. There were localized irregular saccular dilatation and numerous liver flukes in the bile ducts...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28300559/expression-purification-and-in-vitro-refolding-of-the-recombinant-truncated-saposin-like-protein-2-antigen-for-development-of-diagnosis-of-human-fascioliasis
#18
Abolfazl Mirzadeh, Zarrintaj Valadkhani, Asiyeh Yoosefy, Jalal Babaie, Majid Golkar, Ahmad Reza Esmaeili Rastaghi, Elham Kazemi-Rad, Keyhan Ashrafi
Early diagnosis of fascioliasis is critical in prevention of injury to the liver and bile ducts. Saposin-like protein (FhSAP-2) is probably the most ideal antigen of Fasciola hepatica for development of ELISA kits. SAP-2 has a conserved tertiary structure containing three disulfide bonds and conformational epitopes. Therefore, antigenicity of SAP-2 is greatly depends on disulfide bond formation and proper folding. We produced the recombinant truncated SAP-2 (rtSAP-2) in the SHuffle(®) T7 and Rosetta strain of Escherichia coli, in soluble and insoluble forms, respectively and purified by immobilized metal affinity chromatography (IMAC)...
March 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28286139/de-novo-transcriptome-sequencing-and-analysis-of-the-juvenile-and-adult-stages-of-fasciola-gigantica
#19
Xiao-Xuan Zhang, Wei Cong, Hany M Elsheikha, Guo-Hua Liu, Jian-Gang Ma, Wei-Yi Huang, Quan Zhao, Xing-Quan Zhu
Fasciola gigantica is regarded as the major liver fluke causing fasciolosis in livestock in tropical countries. Despite the significant economic and public health impacts of F. gigantica there are few studies on the pathogenesis of this parasite and our understanding is further limited by the lack of genome and transcriptome information. In this study, de novo Illumina RNA sequencing (RNA-seq) was performed to obtain a comprehensive transcriptome profile of the juvenile (42days post infection) and adult stages of F...
March 9, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28285514/fasciola-hepatica-infection-status-of-freshwater-snails-collected-from-gangwon-do-province-korea
#20
Jae-Hyung Lee, Juan-Hua Quan, In-Wook Choi, Gab-Man Park, Guang-Ho Cha, Hyun-Ju Kim, Jae-Min Yuk, Young-Ha Lee
Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2)...
February 2017: Korean Journal of Parasitology
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