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https://www.readbyqxmd.com/read/27906040/identity-of-fasciola-spp-in-sheep-in-egypt
#1
Said Amer, Ahmed ElKhatam, Shereif Zidan, Yaoyu Feng, Lihua Xiao
BACKGROUND: In Egypt, liver flukes, Fasciola spp. (Digenea: Fasciolidae), have a serious impact on the farming industry and public health. Both Fasciola hepatica and Fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. Little is known on the identity and genetic variability of Fasciola populations in sheep. METHODS: This study was performed to determine the prevalence of liver flukes in sheep in Menofia Province as a representative area of the delta region in Egypt, as measured by postmortem examination of slaughtered animals at three abattoirs...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27905284/parasites-determined-by-fecal-examination-in-horses-in-erzurum
#2
Hamza Avcıoğlu, Esin Güven, İbrahim Balkaya, Şevki Yavuz, Uğur Abay, Muzaffer Akyüz, Ömer Eltas
OBJECTIVE: This study aimed to determine the parasites present in horses belonging to the Erzurum Province. METHODS: Fecal samples were collected from 76 horses of different ages, genders and breeds in Erzurum. Individual fecal samples were collected and examined by flotation and sedimentation methods. RESULTS: The following species were detected: strongylid egg (57.89%), Parascaris equorum (10.52%), Dicrocoelium dendriticum (2.63%), Fasciola spp...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27894356/transmission-of-calicophoron-daubneyi-and-fasciola-hepatica-in-galicia-spain-temporal-follow-up-in-the-intermediate-and-definitive-hosts
#3
Javier Iglesias-Piñeiro, Marta González-Warleta, José Antonio Castro-Hermida, María Córdoba, Camino González-Lanza, Yolanda Manga-González, Mercedes Mezo
BACKGROUND: Paramphistomosis caused by Calicophoron daubneyi and fasciolosis caused by Fasciola hepatica are common parasitic diseases of livestock animals. Transmission of the diseases depends on the presence of intermediate hosts, i.e. freshwater gastropods such as lymnaeids. We carried out a 2-year-long study of the dynamics of the snail population acting as the intermediate host for these parasites, considering the population structure in terms of size/age and infection status. In addition, we determined the kinetics of trematode egg excretion in grazing cows...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27890084/comparison-of-molecular-and-conventional-methods-for-the-diagnosis-of-fasciola-hepatica-infection-in-the-field
#4
Maria Immaculata Arifin, Johan Höglund, Adam Novobilský
The liver fluke, Fasciola hepatica, is one of the major parasite threats to livestock industries world-wide. In sheep and cattle, F. hepatica infection is commonly diagnosed using a range of methods. Aside from conventional coprological and serological diagnostic methods, there are also several molecular methods available based on the detection of liver fluke DNA in faeces. In this study, the outcomes of faecal egg count (FEC), serology and coproantigen ELISA (cELISA) were compared with the performance of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) in diagnosis of F...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27890078/animal-fascioliasis-perspectives-from-high-altitudinal-regions
#5
Damanbha Lyngdoh, Sunil Sharma, Bishnupada Roy, Veena Tandon
The parasitic flukes of the genus Fasciola (Platyhelminthes: Trematoda: Digenea) cause fascioliasis or liver-rot disease in ruminant livestock in tropical and sub-tropical regions of the world. Classically, two species of Fasciola- F. hepatica and F. gigantica, are universally recognized as taxonomically valid species. Our survey studies on ovid and bovid animals including yak and mithun from high altitudinal mountainous regions in Northeast India revealed the occurrence of Fasciola gigantica and also Fasciola sp...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27884440/re-infection-with-fasciola-gigantica-6-month-post-treatment-with-triclabendazole-in-cattle-from-mobile-pastoralist-husbandry-systems-at-lake-chad
#6
Helena Greter, Annour A Batil, Idriss O Alfaroukh, Felix Grimm, Bongo N Ngandolo, Jennifer Keiser, Jürg Utzinger, Jakob Zinsstag, Jan Hattendorf
At Lake Chad in central Africa, livestock fascioliasis caused by Fasciola gigantica represents a major veterinary health problem, particularly in cattle reared in mobile pastoralist husbandry systems. We assessed re-infection after a single dose of triclabendazole with fascioliasis in cattle in a mobile pastoralist setting towards the end of the dry season. Within the cattle herds of 14 groups of mobile pastoralists, 375 cattle were randomly selected. A faecal sample was obtained from each animal to determine the prevalence of F...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27884436/distribution-of-foxp3-t-cells-in-the-liver-and-hepatic-lymph-nodes-of-goats-and-sheep-experimentally-infected-with-fasciola-hepatica
#7
A Escamilla, R Zafra, J Pérez, T N McNeilly, I L Pacheco, L Buffoni, F J Martínez-Moreno, V Molina-Hernández, A Martínez-Moreno
Foxp3 regulatory T cells (Tregs) are now considered to play a key role in modulation of immune responses during parasitic helminth infections. Immunomodulation is a key factor in Fasciola hepatica infection; however, the distribution and role of Foxp3(+) Tregs cells have not been investigated in F. hepatica infected ruminants. The aim of this study was to evaluate the presence of Foxp3(+) Tregs in the liver and hepatic lymph nodes from experimentally infected sheep and goats during acute and chronic stages of infection...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27883079/a-parasite-derived-68-mer-peptide-ameliorates-autoimmune-disease-in-murine-models-of-type-1-diabetes-and-multiple-sclerosis
#8
Maria E Lund, Judith Greer, Aakanksha Dixit, Raquel Alvarado, Padraig McCauley-Winter, Joyce To, Akane Tanaka, Andrew T Hutchinson, Mark W Robinson, Ann M Simpson, Bronwyn A O'Brien, John P Dalton, Sheila Donnelly
Helminth parasites secrete molecules that potently modulate the immune responses of their hosts and, therefore, have potential for the treatment of immune-mediated human diseases. FhHDM-1, a 68-mer peptide secreted by the helminth parasite Fasciola hepatica, ameliorated disease in two different murine models of autoimmunity, type 1 diabetes and relapsing-remitting immune-mediated demyelination. Unexpectedly, FhHDM-1 treatment did not affect the proliferation of auto-antigen specific T cells or their production of cytokines...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27876981/detection-of-fasciola-hepatica-and-fasciola-gigantica-common-and-uncommon-antigens-using-rabbit-hyper-immune-serum-raised-against-their-excretory-secretory-and-somatic-antigens
#9
S Abdolahi Khabisi, B Sarkari
Fasciolosis is an important neglected helminth disease caused by two liver flukes, Fasciola hepatica and Fasciola gigantica. The two species of Fasciola are usually different in their morphological and molecular features. They have also common and uncommon antigens in both their somatic and excretory secretory metabolites. In this study, we compared somatic and excretory-secretory (ES) antigens of F. hepatica and F. gigantica, by using rabbit hyper immune serum raised against these antigens. Adult worms were collected from bile ducts of infected animals and species of the fluke was confirmed by RFLP-PCR...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876956/comparison-between-in-house-indirect-elisa-and-dot-elisa-for-the-diagnosis-of-fasciola-gigantica-in-cattle
#10
Jafar Arjmand Yamchi, Nasser Hajipour, Seyyed Meysam Abtahi Froushani, Mojtaba Keighobadi
This survey was done to investigate the efficacy of the in-house indirect ELISA (iELISA) and Dot-ELISA methods Prepared from excretion-secretory (ES Ag) and Crude (Cr Ag) antigens of Fasciola for sero-diagnosis of Fasciola gigantica in cattle. The liver specimens of slaughtered cattle were collected and their liver examined macroscopically and microscopically for infestation to fasciolosis. Sera from two groups of cattle, one infected with fasciolosis (n = 60) and the other non-infected with fasciolosis (n = 60), were used in the iELISA and Dot-ELISA test; grouping based on histopathology results...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876944/seasonal-incidence-of-parasitic-diseases-in-bovines-of-south-western-gujarat-junagadh-india
#11
Binod Kumar, B R Maharana, Amit Prasad, Joice P Joseph, Bhavika Patel, J S Patel
The current study was conducted to investigate the incidence of parasitic diseases in bovines which were sick and brought at veterinary hospital for treatment. A total of 366 samples were investigated from cattle (n = 175) and buffaloes (n = 191) presented at Teaching Veterinary Clinical Complex (TVCC), Veterinary College, Junagadh, Gujarat during January to December 2014. Examination of Giemsa-stained peripheral blood smears exhibited that 58.6 % of cattle and 41.2 % of buffaloes were infected with haemoparasites comprising Babesia bigemina, Theileria annulata, and Anaplasma marginale @ of 54...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876909/prevalence-of-gastrointestinal-parasites-in-bovines-in-and-around-junagadh-gujarat
#12
B R Maharana, Binod Kumar, N R Sudhakar, S K Behera, T K Patbandha
Coprological examination of 416 bovine faecal samples revealed the presence of parasitic stages of Toxocara vitulorum, strongyles, Strongyloides spp., Fasciola spp., amphistomes, coccidia (Eimeria spp. and Cryptosporidium spp.) and Buxtonella sulcata. About 42 % (n = 302) faecal samples from cattle and 36 % (n = 114) samples from buffaloes were positive for gastrointestinal (GI) parasitic infections. Both cattle (14.57 %) and buffalo (15.79 %) had the highest incidence of Buxtonella sulcata, respectively...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27866265/proteomic-analysis-of-fasciola-hepatica-excretory-and-secretory-products-fhesps-involved-in-interacting-with-host-pbmcs-and-cytokines-by-shotgun-lc-ms-ms
#13
Qing Liu, Si-Yang Huang, Dong-Mei Yue, Jin-Lei Wang, Yujian Wang, Xiangrui Li, Xing-Quan Zhu
Fasciola hepatica is a helminth parasite with a worldwide distribution, which can cause chronic liver disease, fasciolosis, leading to economic losses in the livestock and public health in many countries. Control is mostly reliant on the use of drugs, and as a result, drug resistance has now emerged. The identification of F. hepatica genes involved in interaction between the parasite and host immune system is utmost important to elucidate the evasion mechanisms of the parasite and develop more effective strategies against fasciolosis...
November 19, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27862897/a-study-of-the-molluscicidal-and-larvicidal-activities-of-citrullus-colocynthis-l-leaf-extract-and-its-main-cucurbitacins-against-the-mollusc-galba-truncatula-intermediate-host-of-fasciola-hepatica
#14
Rachid Chawech, Fatma Njeh, Nejia Hamed, Mohamed Damak, Ali Ayadi, Hayet Hammami, Raoudha Mezghani-Jarraya
BACKGROUND: The molluscicidal and larvicidal activities of the medicinal plant Citrullus colocynthis leaf extracts and its main cucurbitacins were tested against the mollusc gastropod, Galba truncatula, the intermediate host of Fasciola hepatica. RESULTS: Our findings proved for the first time that the molluscicidal activity was correlated with the presence of terpenoids. A significant molluscicidal value was found in the ethyl acetate extract (LC50 = 12.6 mg/L)...
November 11, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27836038/six-year-longitudinal-study-of-fasciola-hepatica-bulk-milk-antibody-elisa-in-the-dairy-dense-region-of-the-republic-ireland
#15
M P Munita, R Rea, Y Bloemhoff, N Byrne, A M Martinez-Ibeas, R G Sayers
Completion of the F. hepatica lifecycle is dependent on suitable climatic conditions for development of immature stages of the parasite, and its snail intermediate host. Few investigations have been conducted regarding temporal variations in F. hepatica status in Irish dairy herds. The current study aimed to conduct a longitudinal study examining annual and seasonal trends in bulk milk seropositivity over six years, while also investigating associations with soil temperature, rainfall and flukicide treatment...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27821691/recombinase-polymerase-amplification-compared-to-real-time-polymerase-chain-reaction-test-for-the-detection-of-fasciola-hepatica-in-human-stool
#16
Miguel M Cabada, Jose L Malaga, Alejandro Castellanos-Gonzalez, Kelli A Bagwell, Patrick A Naeger, Hayley K Rogers, Safa Maharsi, Maryann Mbaka, A Clinton White
Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens...
November 7, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27809346/on-the-presence-and-immunoregulatory-functions-of-extracellular-micrornas-in-the-trematode-fasciola-hepatica
#17
Bastian Fromm, Vladimir Ovchinnikov, Eirik Høye, Dolores Bernal, Michael Hackenberg, Antonio Marcilla
Liver flukes represent a paraphyletic group of endoparasitic flatworms that significantly affect man either indirectly due to economic damage on livestock or directly as pathogens. A range of studies have focussed on how these macroscopic organisms can evade the immune-system and live inside a hostile environment such as the mammalian liver and bile-ducts. Recently, microRNAs, a class of short non-coding gene-regulators, have been proposed as likely candidates to play roles in this scenario. MicroRNAs (miRNAs) are key players in development and pathogenicity and are highly conserved between metazoans: identical miRNAs can be found in flatworms and mammalians...
November 3, 2016: Parasite Immunology
https://www.readbyqxmd.com/read/27806135/a-trematode-parasite-derived-growth-factor-binds-and-exerts-influences-on-host-immune-functions-via-host-cytokine-receptor-complexes
#18
Azad A Sulaiman, Katarzyna Zolnierczyk, Ornampai Japa, Jonathan P Owen, Ben C Maddison, Richard D Emes, Jane E Hodgkinson, Kevin C Gough, Robin J Flynn
The trematode Fasciola hepatica is responsible for chronic zoonotic infection globally. Despite causing a potent T-helper 2 response, it is believed that potent immunomodulation is responsible for rendering this host reactive non-protective host response thereby allowing the parasite to remain long-lived. We have previously identified a growth factor, FhTLM, belonging to the TGF superfamily can have developmental effects on the parasite. Herein we demonstrate that FhTLM can exert influence over host immune functions in a host receptor specific fashion...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27804894/nuclear-and-mitochondrial-dna-analysis-reveals-that-hybridization-between-fasciola-hepatica-and-fasciola-gigantica-occurred-in-china
#19
Madoka Ichikawa-Seki, Mao Peng, Kei Hayashi, Takuya Shoriki, Uday Kumar Mohanta, Toshiyuki Shibahara, Tadashi Itagaki
The well-known pathogens of fasciolosis, Fasciola hepatica (Fh) and Fasciola Gigantica (Fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. On the other hand, aspermic Fasciola flukes reported from Asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. The aim of this study was to reveal the origin of aspermic Fasciola flukes. The nuclear single copy markers, phosphoenolpyruvate carboxykinase and DNA polymerase delta, were employed for analysis of Fasciola species from China...
November 2, 2016: Parasitology
https://www.readbyqxmd.com/read/27799075/rumen-fluke-calicophoron-daubneyi-on-welsh-farms-prevalence-risk-factors-and-observations-on-co-infection-with-fasciola-hepatica
#20
Rhys Aled Jones, Peter M Brophy, E Sian Mitchell, Hefin Wyn Williams
Reports of Calicophoron daubneyi infecting livestock in Europe have increased substantially over the past decade; however, there has not been an estimate of its farm level prevalence and associated risk factors in the UK. Here, the prevalence of C. daubneyi across 100 participating Welsh farms was recorded, with climate, environmental and management factors attained for each farm and used to create logistic regression models explaining its prevalence. Sixty-one per cent of farms studied were positive for C...
November 1, 2016: Parasitology
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