keyword
MENU ▼
Read by QxMD icon Read
search

Fasciola

keyword
https://www.readbyqxmd.com/read/29161330/fasciola-spp-mapping-of-the-mf6-epitope-and-antigenic-analysis-of-the-mf6p-hdm-family-of-heme-binding-proteins
#1
Victoria Martínez-Sernández, María J Perteguer, Mercedes Mezo, Marta González-Warleta, Teresa Gárate, M Adela Valero, Florencio M Ubeira
MF6p/FhHDM-1 is a small cationic heme-binding protein which is recognized by the monoclonal antibody (mAb) MF6, and abundantly present in parenchymal cells and secreted antigens of Fasciola hepatica. Orthologs of this protein (MF6p/HDMs) also exist in other causal agents of important foodborne trematodiasis, such as Clonorchis sinensis, Opisthorchis viverrini and Paragonimus westermani. Considering that MF6p/FhHDM-1 is relevant for heme homeostasis in Fasciola and was reported to have immunomodulatory properties, this protein is expected to be a useful target for vaccination...
2017: PloS One
https://www.readbyqxmd.com/read/29160354/dynamics-of-haemocytes-from-pseudosuccinea-columella-circulating-infected-by-fasciola-hepatica
#2
Vinicius Marques Antunes Ribeiro, Aytube Lucas Coaglio, Fernando Luiz Pereira Oliveira, Cíntia Aparecida de Jesus Pereira, Walter Dos Santos Lima
The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi). Miracidia were observed head-foot and mantle at 30 minutes post-infection...
November 9, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29158867/hepatic-fascioliasis-presenting-with-bile-duct-obstruction-a-case-report
#3
Rachid Lefryekh, Ahmed Bensaad, Fatimazahra Bensardi, Khalid Elhattabi, Mounir Bouali, Bessam Daif, Abdelaziz Fadil, Zakaria Jaouhari, Tazi Hicham, Aziz Hamdani, Maha Soussi Abdalaoui
Fascioliasis is a zoonotic infection caused by a liver trematode: fasciola hepatica; which commonly affects cattle and sheep, humans are accidental hosts. Several cases have been reported in the literature worldwide with a large geographical distribution. We present a case of bile duct obstruction due to a hepatic fascioliasis, successfully treated with both a combined surgical and medical approaches. A high index of suspicion should be kept in mind for all cases of obstructive jaundice, especially in areas in which human fascioliasis infection is repeatedly reported...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29152559/fasciola-hepatica-esps-could-indistinctly-activate-or-block-multiple-toll-like-receptors-in-a-human-monocyte-cell-line
#4
Olgary Figueroa-Santiago, Ana M Espino
Fasciola hepatica is a parasitic helminth that induces Th2/Treg responses in its mammalian host. Some reports have suggested that ESPs achieve these polarized immune responses by delaying the activation of dendritic cells and macrophages during the early stages of innate immunity, a process that is mediated by TLR4. The present study aimed to investigate whether TLRs other than TLR4 could also be targeted by F. hepatica ESPs. To achieve this aim a screening system was optimized using THP1-Blue CD14 cells. ESPs were first separated based on their molecular weight and according their net charge by ion exchange chromatography (IEC)...
2017: Ann Clin Pathol
https://www.readbyqxmd.com/read/29151443/fasciola-hepatica-infection-in-water-buffalo-bubalus-bubalis-in-three-provinces-of-the-nile-delta-egypt-a-cross-sectional-study
#5
Abdelgawad Salah El-Tahawy, Nigel Kwan, Katsuaki Sugiura
A cross-sectional study was conducted to estimate the prevalence of Fasciola hepatica (F. hepatica) infection in water buffalo (Bubalus bubalis) in Alexandria, Beheira, and Kafr el-Sheikh governorates (provinces) of the Nile Delta in Egypt and to identify the underlying risk factors associated with the infection. A total of 29 farms (10 in Alexandria, 10 in Beheira, and 9 in Kafr el-Sheikh) were randomly selected and all the buffaloes that resided on these farms from 21 February 2015 to 20 February 2016 were included in the study...
November 20, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29143720/epidemiological-investigation-of-a-severe-rumen-fluke-outbreak-on-an-irish-dairy-farm
#6
James O'Shaughnessy, Andres Garcia-Campos, Conor G McAloon, Seamus Fagan, Theo de Waal, Maire McElroy, Micheal Casey, Barbara Good, Grace Mulcahy, John Fagan, Denise Murphy, Annetta Zintl
Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochrome oxidase subunit 1 (Cox1) gene of the rumen fluke metacercariae on pasture failed to identify predominant, possibly pathogenic subtypes. However, estimates of metacercarial load indicated that the animals were exposed to a daily dose of about 5334 C...
November 16, 2017: Parasitology
https://www.readbyqxmd.com/read/29132905/purification-and-biochemical-characterization-of-a-22-kda-stable-cysteine-like-protease-from-the-excretory-secretory-product-of-the-liver-fluke-fasciola-hepatica-by-using-conventional-techniques
#7
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
This study describes the purification and characterization of a stable protease activity isolated from Fasciola hepatica adult worms maintained in vitro by employing acetone precipitation (40-60%) followed by a gel filtration through Sephadex G-100 and DEAE- cellulose ion exchange column. Through this three-step purification, the enzyme was purified 11-fold with a specific activity of 1893.9U/mg and 31.5% recovery. After the final ultrafiltration step, the purification fold was increased up to 13.1 and the overall activity yield reached a rate of 18...
October 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29121986/farm-level-risk-factors-for-fasciola-hepatica-infection-in-danish-dairy-cattle-as-evaluated-by-two-diagnostic-methods
#8
Nao Takeuchi-Storm, Matthew Denwood, Tina Vicky Alstrup Hansen, Tariq Halasa, Erik Rattenborg, Jaap Boes, Heidi Larsen Enemark, Stig Milan Thamsborg
BACKGROUND: The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA) as a tool for diagnosis in Danish dairy cattle farms was assessed. METHODS: This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29118312/dynamics-of-liver-enzymes-in-rabbits-experimentally-infected-with-fasciola-sp-intermediate-form-from-japan
#9
Wipaporn Jarujareet, Kensuke Taira, Hong Kean Ooi
Dynamics of serum liver enzymes in rabbits experimentally infected with metacercariae of Fasciola sp. (intermediate form between Fasciola hepatica and F. gigantica) were monitored. Gradual increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed from 3 weeks post-inoculation (WPI) and peaked at 6 WPI, which corresponded well to the period of migration and development of juvenile fluke in the liver parenchyma and the time when the young adult flukes migrated to the bile duct...
November 9, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29114128/efficacy-of-glutathione-s-transferase-purified-antigen-of-the-gastro-intestinal-nematode-haemonchus-contortus-in-diagnosis-of-sheep-haemonchosis
#10
Omnia M Kandil, Irene S Gamil, Seham H M Hendawy, Fatma Medhat, Ola H El-Habit
Haemonchus contortus is one of the most pathogenic and economically important parasites of sheep. Different H. contortus antigens; crude somatic antigen (CSA), excretory/secretory antigen (ESA), crude larval antigen (CLA), glutathione-S-transferase antigen (GST) and recombinant protein (rhcp 26/23) were prepared and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot. The antigens were immunologically evaluated through indirect enzyme linked immunosorbent assay (ELISA) for diagnosis of haemonchosis in experimentally and naturally infected sheep...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29111192/antifungal-molluscicidal-and-larvicidal-assessment-of-anemonin-and-clematis-flammula-l-extracts-against-mollusc-galba-truncatula-intermediate-host-of-fasciola-hepatica-in-tunisia
#11
Rakia Saidi, Lamia Khanous, Safa Khadim Allah, Besma Hamdi, Ali Ayadi, Mohamed Damak, Hayet Hammami, Raoudha Mezghani-Jarraya
OBJECTIVE: To investigate the potential of anemonin and Clematis flammula (C. flammula) extracts against infective organisms. METHODS: The molluscicidal activities of anemonin and C. flammula extracts against Galba truncatula Müll. (Lymnaeidae) and Fasciola hepatica larval stages contaminating this snail in Tunisia were assessed by testing six groups of snails in 250 mL of extracts and aqueous dechlorinated solutions with different concentrations (ranging from 2...
October 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29052925/structural-insights-into-natural-compounds-as-inhibitors-of-fasciola-gigantica-thioredoxin-glutathione-reductase
#12
Rohit Shukla, Harish Shukla, Parismita Kalita, Timir Tripathi
Fascioliasis is caused by the helminth parasites of genus Fasciola. Thioredoxin glutathione reductase (TGR) is an important enzyme in parasitic helminths and plays an indispensable role in its redox biology. In the present study, we conducted a structure-based virtual screening of natural compounds against the Fasciola gigantica TGR (FgTGR). The compounds were docked against FgTGR in four sequential docking modes. The screened ligands were further assessed for Lipinski and ADMET prediction so as to evaluate drug proficiency and likeness property...
October 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29044374/human-and-livestock-trematode-infections-in-a-mobile-pastoralist-setting-at-lake-chad-added-value-of-a-one-health-approach-beyond-zoonotic-diseases-research
#13
Helena Greter, Annour A Batil, Bongo N Ngandolo, Idriss O Alfaroukh, Doumagoum D Moto, Jan Hattendorf, Jürg Utzinger, Jakob Zinsstag
Background: At Lake Chad in Central Africa, mobile pastoralists face economic losses due to livestock trematodiases. Fasciola gigantica and Schistosoma bovis-trematodes that affect livestock-share transmission ecology traits with Schistosoma haematobium and S. mansoni that cause human schistosomiasis. This project aimed at assessing treatment strategies and elucidating the predictive potential of human and livestock trematode infections. Methods: Schistosomiasis and fascioliasis were investigated concurrently in humans and cattle by repeated cross-sectional surveys...
June 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29031680/soluble-expression-and-purification-of-a-full-length-asparaginyl-trna-synthetase-from-fasciola-gigantica
#14
R Vijayakumar, Timir Tripathi
We report the molecular cloning, expression, and single-step homogeneous purification of a full-length asparaginyl tRNA synthetase (NRS) from Fasciola gigantica (FgNRS). Fasciola gigantica is a parasitic liver fluke of the class Trematoda. It causes fascioliasis that infects the liver of various mammals, including humans. Aminoacyl tRNA synthetases (AARS) catalyze the first step of protein synthesis. They attach an amino acid to its cognate tRNA, forming an amino acid-tRNA complex. The gene that codes for FgNRS was generated by amplification by polymerase chain reaction...
October 12, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29027596/10-year-parasitological-examination-results-2003-to-2012-of-faecal-samples-from-horses-ruminants-pigs-dogs-cats-rabbits-and-hedgehogs
#15
Katharina Raue, Lea Heuer, Claudia Böhm, Sonja Wolken, Christian Epe, Christina Strube
The results of coproscopical examinations in domestic animals and hedgehogs carried out as routine diagnostics in the years 2003 to 2012 at the Institute for Parasitology, University of Veterinary Medicine Hannover, Germany, are presented. Of 3475 horse faecal samples, 30.1% contained stages of strongyles and 1.3% eggs of Strongyloides westeri and Parascaris equorum, respectively. The most frequently observed parasite stages in 1416 cattle faecal samples were Eimeria oocysts (21.3%) and strongyle eggs or larvae (15...
December 2017: Parasitology Research
https://www.readbyqxmd.com/read/29023077/spermatogenic-and-phylo-molecular-characterizations-of-isolated-fasciola-spp-from-cattle-north-west-iran
#16
Soheila Rouhani, Saber Raeghi, Adel Spotin
BACKGROUND AND OBJECTIVE: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. MATERIALS AND METHODS: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29016352/in-vitro-ovicidal-activity-of-poly-lactic-acid-curcumin-nisin-co-entrapped-nanoparticle-against-fasciola-spp-eggs-and-its-reproductive-toxicity
#17
Oyetunde Oyeyemi, Odunayo Adegbeyeni, Ifeoluwa Oyeyemi, Jairam Meena, Amulya Panda
BACKGROUND: Curcumin and nisin have been widely reported for their antibacterial and anticancer potency. However, their therapeutic applications are hampered by several factors, which necessitate their development into nanosize ranges for improved delivery and activities. Their incorporation into a single nanosynthesized form may suggest desirable efficacy on parasites. The aim of the study was to assess the ovicidal activity of the curcumin-nisin polylactic acid (PLA) entrapped nanoparticle on the Fasciola eggs and its reproductive toxicity...
October 10, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28993458/dairy-heifers-naturally-exposed-to-fasciola-hepatica-develop-a-type-2-immune-response-and-concomitant-suppression-of-leukocyte-proliferation
#18
John Graham-Brown, Catherine Hartley, Helen Clough, Aras Kadioglu, Matthew Baylis, Diana J L Williams
Fasciola hepatica is a parasitic trematode of global importance in livestock. Control strategies reliant on anthelmintics are unsustainable due to the emergence of drug resistance. Vaccines are under development, but efficacy is variable. Evidence from experimental infection suggest vaccine efficacy may be affected by parasite-induced immunomodulation. Little is known about the immune response to F. hepatica following natural exposure. Hence we analysed the immune responses over time in calves naturally exposed to F...
October 9, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28984428/fasciola-and-fasciolosis-in-ruminants-in-europe-identifying-research-needs
#19
N J Beesley, C Caminade, J Charlier, R J Flynn, J E Hodgkinson, A Martinez-Moreno, M Martinez-Valladeres, J Perez, L Rinaldi, D J L Williams
Fasciola hepatica is a trematode parasite with a global distribution, which is responsible for considerable disease and production losses in a range of food producing species. It is also identified by WHO as a re-emerging neglected tropical disease associated with endemic and epidemic outbreaks of disease in human populations. In Europe, F. hepatica is mostly associated with disease in sheep, cattle and goats. This study reviews the most recent advances in our understanding of the transmission, diagnosis, epidemiology and the economic impact of fasciolosis...
October 6, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28979349/seroprevalence-of-human-fasciolosis-in-lorestan-province-western-iran-in-2015-16
#20
Peyman Heydarian, Keyhan Ashrafi, Mehdi Mohebali, Eshrat Beigom Kia, Mojgan Aryaeipour, Ali Chegeni Sharafi, Hamid Mokhayeri, Arezoo Bozorgomid, Mohammad Bagher Rokni
BACKGROUND: The aim of this study was the seroepidemiological survey for detecting the status of human fasciolosis in Lorestan Province, western Iran. METHODS: This cross-sectional study was conducted in 2015-16. Based on statistical estimations, 1256 serum samples were collected from different parts of Lorestan Province, western Iran, and stored at -20 °C until use. The collected serum samples were analyzed at Tehran University of Medical Sciences, Tehran, Iran using indirect ELISA method...
July 2017: Iranian Journal of Parasitology
keyword
keyword
34911
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"