Read by QxMD icon Read


Christine Schikowsky, Jennifer Senkler, Hans-Peter Braun
The succinate dehydrogenase complex (complex II) is a highly conserved protein complex composed of the SDH1 to SDH4 subunits in bacteria and in the mitochondria of animals and fungi. The reason for the occurrence of up to four additional subunits in complex II of plants, termed SDH5 to SDH8, so far is a mystery. Here, we present a biochemical approach to investigate the internal subunit arrangement of Arabidopsis (Arabidopsis thaliana) complex II. Using low-concentration detergent treatments, the holo complex is dissected into subcomplexes that are analyzed by a three-dimensional gel electrophoresis system...
February 2017: Plant Physiology
Mi Young Ahn, Min-Ji Kim, Ryun Hee Kwon, Jae Sam Hwang, Kun-Koo Park
BACKGROUND: Molecular genetic mechanisms underlying the anti-inflammatory effects of ethanol extract (GB) from G. bimaculatus, a type of cricket, are not fully elucidated. G. bimaculatus was reported to be rich in unsaturated fatty acid and to decrease the omega-6/omega-3 fatty acid ratio when fed to chickens. GB may reduce the amount of fat or increase the unsaturated fatty acid ratio. METHODS: Male Wistar rats fed a high-fat diet (HFD) were orally administered with 5 groups: phosphate buffered saline (PBS, control), GB (100 mg/kg or 200 mg/kg), Pravastatin or Isaria sinclairii (IS) extract, which is reported to have fat-reducing effects, for either 1 or 2 months...
2015: Lipids in Health and Disease
Suresh T Mathews, Emily Graff, Robert L Judd, Vishal Kothari
The western blotting technique for transfer and detection of proteins, named following the discovery of southern and northern blotting for DNA- and RNA-blotting, respectively, has traditionally relied on the use of X-ray films to capture chemiluminescence. Recent advancements use super-cooled charge coupled devices (CCD) cameras to capture both chemiluminescence and fluorescence images, which exhibit a greater dynamic range compared to traditional X-ray film. Chemiluminescence detected by a CCD camera records photons and displays an image based on the amount of light generated as a result of a dynamic chemical reaction...
2015: Methods in Molecular Biology
J P Liu, X W Wang, L P Qie
We analyzed disease severity, inflammation markers, and dynamic changes in cartilage glycoprotein 39 (YKL-40) and C-reactive protein (CRP) levels in children with sepsis before and after treatment with continuous blood purification (CBP). Study participants were 30 children with severe sepsis who were cured from the disease (experimental group) in the Children's Serious Disease Center of In-ner Mongolia People's Hospital between June 2012 and October 2013. Symptomatic CBP treatment was performed after disease severity scoring...
May 25, 2015: Genetics and Molecular Research: GMR
S Shehata, W Richter, M Schuster, H Lindermayer
The study examined effect of two adsorbents on the toxicity of Deoxynivalenol (DON) in growing pigs in a feeding trial. 24 male growing pigs (average initial body weight 11.5 kg) were assigned to one of six dietary treatments: control (uncontaminated diet); control + 0.5% adsorbent I; DON contaminated diet (1.73 mg/kg); DON contaminated diet + 0.5% adsorbent I; control + 0.5% adsorbent II and DON contaminated diet + 0.5% adsorbent II. Two digestibility trials were conducted on the second and fourth week of the feeding period with a sampling period of 7 days to determine the digestibility of the nutrients and the amounts of DON in faeces and urine...
March 2004: Mycotoxin Research
L M Hulme, K A Atkinson, R H Clothier, M Balls
A number of in vitro systems have been put forward as potential alternative methods for testing chemicals for teratogenic potential. The most promising of these systems, for example mammalian whole embryo culture and the micromass technique, are currently undergoing further interlaboratory validation. However, such tests involve the use of a considerable number of animals. It was therefore decided to investigate the possible use of a permanent cell line that possessed many of the properties of embryonic cells, that is a differentiating cell line, F9 (derived from a mouse teratocarcinoma), in the development of an in vitro teratogenicity test...
1990: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Iu A Kudriavtseva, I Iu Zhuravleva, A T Titiov, L S Barbarash
The present study was undertaken to experimentally assess a possibility of creating a xenogeneic biological venous-valve prosthetic graft using the vv. saphena medialis of the cattle. The findings obtained in the amino-acid analysis demonstrated that diglycidyl ether ofethylene glycol (DEEG) is an efficient suturing agent for xenoveins. Suturing collagen with DEEG by qualitative and quantitative characteristics appeared superior to treatment with glutaric aldehyde (GA); the content of hydroxylysine was by 30%, and that of lysine by 360% less than in the samples treated with GA...
2008: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
S Mitrovska, S Jovanova
OBJECTIVES: We compared the incidence of adverse cardiac outcomes of enoxaparin vs unfractionated heparin in the management of ACS-NSTE. BACKGROUND: Low-molecular-weight heparins are the potential new standard in the treatment of acute coronary syndromes without ST-segment elevation (ACS-NSTE). The benefit is addressed to significant diminution of the adverse clinical events--recurrent angina (RA), myocardial infarction (MI), heart failure (HF), cerebrovascular insult (ICV), coronary artery by-pass graft (CABG), percutaneous coronary intervention (PCI) and death...
2009: Bratislavské Lekárske Listy
Suresh T Mathews, Eric P Plaisance, Teayoun Kim
Western blot detection methods have traditionally used X-ray films to capture chemiluminescence. The increasing costs for film, reagents, and maintenance have driven researchers away from darkrooms to more sensitive and technologically advanced digital imaging systems. Cooled charge coupled devices (CCD) cameras capture both chemiluminescence and fluorescence images, with limitations for each detection method. Chemiluminescence detection is highly sensitive and relies on an enzymatic reaction that produces light, which can be detected by a CCD camera that records photons and displays an image based on the amount of light generated...
2009: Methods in Molecular Biology
Axel Helmstädter
The German Physician Wilhelm Schüssler (1821-1898) developed a healing system called "Biochemie". Basically it had merely the character of a substitution therapy with mineral compounds and was based on physiological considerations by Jakob Moleschott (1822-1893) and Justus von Liebig (1803-1873) which were fundamental for the development of the scientific discipline of biochemistry as well. Schüssler himself, however, who had been educated in homoeopathy, introduced more and more immaterial or "biodynamic" explanations for the therapeutic effect of the mineral salt preparations...
2006: Gesnerus
S Mitrovska, S Jovanova
OBJECTIVE: The primary objective of this study is to analyse relationship between activated partial thromboplastin time (aPTT) and clinical events in patients with unstable coronary artery disease. BACKGROUND: Unfractionated heparin (UFH) is considered as a standard therapy in the management of unstable coronary artery disease. His efficacy is concerned to low rate of adverse events, but it shows lack of correlation between aPTT and clinical outcomes. METHODS: Thirty patients with acute coronary syndromes were randomised to receive weight-adjusted unfractionated heparin (Heparin-"Biochemie" 25...
2006: Bratislavské Lekárske Listy
Jean-Paul Gaudillière
This paper follows the trajectory of sex steroids in 1930s Germany as a way to investigate the system of research which characterized the development of these drugs. Analyzing the changing relationship between the pharmaceutical company Schering and the Kaiser Wilhelm Institute für Biochemie headed by Nobel Prize winner Adolf Butenandt, the paper highlights the circulation of materials, information and money as much as the role of patents in shaping the study of sex steroids. Semi-synthetic analogs and metabolic pathways thus emerged as shared bio-industrial assets...
December 2005: Studies in History and Philosophy of Biological and Biomedical Sciences
Nicolas Dumaz, Richard Marais
One of the hallmarks of cAMP is its ability to inhibit proliferation in many cell types, but stimulate proliferation in others. Clearly cAMP has cell type specific effects and the outcome on proliferation is largely attributed to crosstalk from cAMP to the RAS/RAF/mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK pathway. We review the crosstalk between these two ancient and conserved pathways, describing the molecular mechanisms underlying the interactions between these pathways and discussing their possible biological importance...
July 2005: FEBS Journal
Oliver Hofmann, Dietmar Schomburg
MOTIVATION: Given the explosive growth of biomedical data as well as the literature describing results and findings, it is getting increasingly difficult to keep up to date with new information. Keeping databases synchronized with current knowledge is a time-consuming and expensive task-one which can be alleviated by automatically gathering findings from the literature using linguistic approaches. We describe a method to automatically annotate enzyme classes with disease-related information extracted from the biomedical literature for inclusion in such a database...
May 1, 2005: Bioinformatics
No abstract text is available yet for this article.
March 29, 1963: Deutsche Medizinische Wochenschrift
No abstract text is available yet for this article.
December 1957: Bollettino Della Società Italiana di Biologia Sperimentale
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"