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https://www.readbyqxmd.com/read/27932642/complete-genome-sequence-of-vb_ecom-ufv13-a-new-bacteriophage-able-to-disrupt-trueperella-pyogenes-biofilm
#1
Vinícius S Duarte, Roberto S Dias, Andrew M Kropinski, Pedro M P Vidigal, Flávia O Sousa, André S Xavier, Cynthia C Silva, Sergio O de Paula
vB_EcoM-UFV13, a member of the T4virus genus, shows lytic activity against Escherichia coli and effectiveness in controlling the biofilm formed by Trueperella pyogenes, which qualifies it as a promising component of phage cocktails for mastitis and metritis control.
December 8, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27932636/complete-genome-sequence-of-bacteriophage-ma12-which-infects-both-campylobacter-jejuni-and-salmonella-enterica-serovar-enteritidis
#2
Sunjin Lee, Taesoo Kwon, Su-Jin Chae, Jong-Hyun Kim, Yeon Ho Kang, Gyung Tae Chung, Dae-Won Kim, Deog-Yong Lee
Here, we announce the complete genome sequence of Salmonella enterica serovar Enteritidis (S Enteritidis) bacteriophage MA12, a 41-Kb chromosome. The strain can infect both Campylobacter jejuni (C. jejuni) and S Enteritidis and can be used in phage therapy experiments with poultry and poultry meat.
December 8, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27930489/screening-and-preliminary-verification-of-a-phage-display-single-chain-antibody-library-against-coal-workers-pneumoconiosis
#3
Guang-Hui Zhang, Li Li, Changfu Hao, Jing-Chao Ren, Hongyi Zhang, Jie Jiao, Liyun Gao, Shibin Ding, Sanqiao Yao, Wu Yao, Weidong Wu
OBJECTIVES: To construct a phage display human antibody library (PDHAL) against pneumoconiosis for the diagnosis and treatment of coal worker pneumoconiosis (CWP). METHODS: The PDHAL was established via CWP blood and six positive antibodies were discovered. 867 coal workers (558 CWP and 309 without CWP) and 393 controls were recruited to validate the results. RESULTS: A PDHAL against CWP was established, from which six strong positive clones were selected, sequenced and identified as VEGF, interleukin-18, HSP70, HER3, Gz-B and RF...
December 2016: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/27929745/an-elaborate-landscape-of-the-human-antibody-repertoire-against-enterovirus-71-infection-is-revealed-by-phage-display-screening-and-deep-sequencing
#4
Zhe Chen, Xianwen Ren, Jian Yang, Jie Dong, Ying Xue, Lilian Sun, Yafang Zhu, Qi Jin
Enterovirus 71 (EV71) causes outbreaks of hand, foot and mouth disease (HFMD), primarily in the Asia-Pacific area, that are often associated with complications of severe to fatal neurological symptoms. There are currently no anti-viral therapies or vaccines available for the treatment of EV71 infection. Illustrating human antibody responses neutralizing EV71 infection could potentially provide valuable information for the development of effective therapies and vaccines. Here, we constructed a comprehensive phage display library based on peripheral blood of eight EV71-infected donors and identified 27 EV71-specific human antibodies, of which four have neutralizing activity in in vitro experiments...
December 8, 2016: MAbs
https://www.readbyqxmd.com/read/27929733/antibody-repertoire-profiling-with-mimotope-arrays
#5
Shina Pashova, Christoph Schneider, Stephan von Gunten, Anastas Pashov
Large-scale profiling and monitoring of antibody repertoires is possible through next generation sequencing (NGS), phage display libraries and microarrays. These methods can be combined in a pipeline, which ultimately maps the antibody reactivities onto defined arrays of structures - peptides or carbohydrates. The arrays can help analyze the individual specificities or can be used as complex patterns. In any case, the targets recognized should formally be considered mimotopes unless they are proven to be epitopes driving the antibody synthesis...
December 8, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27928961/darpins-bioengineering-and-its-theranostic-approaches-emerging-trends-in-protein-engineering
#6
Mahmood Rasool, Arif Malik, Mubashir Hussain, Kalsoom A Haq, Kiran Butt, Abdul Basit, Muhammad Imran Naseer, Muhammad Asif, Rozena Shaikh, Mohammad Zahid Mustafa, Qamre Alam, Ghulam Rasool, Waseem Ahmad, Absarul L Haque, Mohammad Amjad Kamal
The therapeutic significance of bioengineering proteins has increased dramatically as a new generation of pharmacological drug with a great potential in medical treatment. Protein engineering has improved the use of new non immunoglobulin affinity proteins. The designed ankyrin repeat proteins abbreviated as DARPins is an example. The molecules have smaller structural size, unlike immunoglobulins, but similar target affinity is of great significance. Such molecules get more binding specificity and greater thermodynamic stability...
December 8, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27925169/parasites-and-competitors-suppress-bacterial-pathogen-synergistically-due-to-evolutionary-trade-offs
#7
Xiaofang Wang, Zhong Wei, Mei Li, Xueqi Wang, Anqi Shan, Xinlan Mei, Alexandre Jousset, Qirong Shen, Yangchun Xu, Ville-Petri Friman
Parasites and competitors are important for regulating pathogen densities and subsequent disease dynamics. It is, however, unclear to what extent this is driven by ecological and evolutionary processes. Here we used experimental evolution to study the eco-evolutionary feedbacks between Ralstonia solanacearum bacterial pathogen, Ralstonia-specific phage parasite and Bacillus amyloliquefaciens competitor bacterium in the laboratory and plant rhizosphere. We found that while the phage had a small effect on pathogen densities on its own, it considerably increased the R...
December 7, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27924784/bio-fabrication-of-nanomesh-channels-of-single-walled-carbon-nanotubes-for-locally-gated-field-effect-transistors
#8
Hye-Hyeon Byeon, Woo Chul Lee, Wonbin Kim, Seong Keun Kim, Woong Kim, Hyunjung Yi
Single-walled carbon nanotubes (SWNTs) are one of the promising electronic components for nanoscale electronic devices such as field-effect transistors (FETs) owing to their excellent device characteristics such as high conductivity, high carrier mobility and mechanical flexibility. Localized gating gemometry of FETs enables individual addressing of active channels and allows for better electrostatics via thinner dielectric layer of high k-value. For localized gating of SWNTs, it becomes critical to define SWNTs of controlled nanostructures and functionality onto desired locations in high precision...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27924153/genome-sequence-of-a-commensal-bacterium-enterococcus-faecalis-cba7120-isolated-from-a-korean-fecal-sample
#9
Joon Yong Kim, Hye Seon Song, Yeon Bee Kim, Joseph Kwon, Jong-Soon Choi, Yong-Joon Cho, Byung-Yong Kim, Jin-Kyu Rhee, Jinjong Myoung, Young-Do Nam, Seong Woon Roh
BACKGROUND: Enterococcus faecalis, the type strain of the genus Enterococcus, is not only a commensal bacterium in the gastrointestinal tract in vertebrates and invertebrates, but also causes serious disease as an opportunistic pathogen. To date, genome sequences have been published for over four hundred E. faecalis strains; however, pathogenicity of these microbes remains complicated. To increase our knowledge of E. faecalis virulence factors, we isolated strain CBA7120 from the feces of an 81-year-old female from the Republic of Korea and performed a comparative genomic analysis...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27923713/when-transcription-goes-on-holliday-double-holliday-junctions-block-rna-polymerase-ii-transcription-in-vitro
#10
Anne Pipathsouk, Boris P Belotserkovskii, Philip C Hanawalt
Non-canonical DNA structures can obstruct transcription. This transcription blockage could have various biological consequences, including genomic instability and gratuitous transcription-coupled repair. Among potential structures causing transcription blockage are Holliday junctions (HJ), which can be generated as intermediates in homologous recombination or during processing of stalled replication forks. Of particular interest is the double Holliday junction (DHJ), which contains two HJs. Topological considerations impose the constraint that the total number of helical turns in the DNA duplexes between the junctions cannot be altered as long as the flanking DNA duplexes are intact...
December 3, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27923541/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme-trends-in-microbiology-25-first-page-en-dash-last-page-2017
#11
Riccardo Manganelli, Maria Laura Gennaro
No abstract text is available yet for this article.
December 3, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27921283/choices-have-consequences-the-nexus-between-dna-repair-pathways-and-genomic-instability-in-cancer
#12
REVIEW
Sonali Bhattacharjee, Saikat Nandi
BACKGROUND: The genome is under constant assault from a multitude of sources that can lead to the formation of DNA double-stand breaks (DSBs). DSBs are cytotoxic lesions, which if left unrepaired could lead to genomic instability, cancer and even cell death. However, erroneous repair of DSBs can lead to chromosomal rearrangements and loss of heterozygosity, which in turn can also cause cancer and cell death. Hence, although the repair of DSBs is crucial for the maintenance of genome integrity the process of repair need to be well regulated and closely monitored...
December 2016: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/27920385/sublethal-streptomycin-concentrations-and-lytic-bacteriophage-together-promote-resistance-evolution
#13
Johannes Cairns, Lutz Becks, Matti Jalasvuori, Teppo Hiltunen
Sub-minimum inhibiting concentrations (sub-MICs) of antibiotics frequently occur in natural environments owing to wide-spread antibiotic leakage by human action. Even though the concentrations are very low, these sub-MICs have recently been shown to alter bacterial populations by selecting for antibiotic resistance and increasing the rate of adaptive evolution. However, studies are lacking on how these effects reverberate into key ecological interactions, such as bacteria-phage interactions. Previously, co-selection of bacteria by phages and antibiotic concentrations exceeding MICs has been hypothesized to decrease the rate of resistance evolution because of fitness costs associated with resistance mutations...
January 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/27919889/study-of-intracellular-staphylococcus-aureus-control-by-virulent-bacteriophage-within-mac-t-bovine-mammary-epithelial-cells
#14
Lili Zhang, Lichang Sun, Ruicheng Wei, Qiang Gao, Tao He, Cunfa Xu, Xianjin Liu, Ran Wang
Bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureus, in a time-dependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within the 3 h post-incubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies, and may protect from recurrent infection and treatment failure...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27917795/lytic-activity-of-various-phage-cocktails-on-multidrug-resistant-bacteria
#15
I Ozkan, E Akturk, N Yeshenkulov, S Atmaca, N Rahmanov, H I Atabay
PURPOSE: A bacteriophage is a virus that infects and replicates within a bacterium following the injection of phage genome into the bacterial cytoplasm. They are seen as a possible therapy for multi-drug-resistant strains of many bacteria. The aim of this study is to evaluate the lytic activity of the Pyo, Intesti and Fersisi bacteriophage cocktails on P. aeruginosa and S. aureus. METHODS: Ten different S. aureus and P. aeruginosa strains, which were isolated from hospitalized patients in Turkey, were used in the study...
December 1, 2016: Clinical and Investigative Medicine. Médecine Clinique et Experimentale
https://www.readbyqxmd.com/read/27917276/preparation-and-characterization-of-a-novel-nanobody-against-t-cell-immunoglobulin-and-mucin-3-tim-3
#16
Vida Homayouni, Mazdak Ganjalikhani-Hakemi, Abbas Rezaei, Hossein Khanahmad, Mahdi Behdani, Fatemeh Kazemi Lomedasht
OBJECTIVES: As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. MATERIALS AND METHODS: We immunized a camel with TIM-3 antigen and then, synthesized a VHH phage: mid library from its B cell's transcriptome using nested PCR...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27916936/capsule-targeting-depolymerase-derived-from-klebsiella-kp36-phage-as-a-tool-for-the-development-of-anti-virulent-strategy
#17
Grażyna Majkowska-Skrobek, Agnieszka Łątka, Rita Berisio, Barbara Maciejewska, Flavia Squeglia, Maria Romano, Rob Lavigne, Carsten Struve, Zuzanna Drulis-Kawa
The rise of antibiotic-resistant Klebsiella pneumoniae, a leading nosocomial pathogen, prompts the need for alternative therapies. We have identified and characterized a novel depolymerase enzyme encoded by Klebsiella phage KP36 (depoKP36), from the Siphoviridae family. To gain insights into the catalytic and structural features of depoKP36, we have recombinantly produced this protein of 93.4 kDa and showed that it is able to hydrolyze a crude exopolysaccharide of a K. pneumoniae host. Using in vitro and in vivo assays, we found that depoKP36 was also effective against a native capsule of clinical K...
December 1, 2016: Viruses
https://www.readbyqxmd.com/read/27916124/alkaline-phosphatase-fused-repebody-as-a-new-format-of-immuno-reagent-for-an-immunoassay
#18
Hyo-Deok Seo, Joong-Jae Lee, Yu Jung Kim, Oliver Hantschel, Seung-Goo Lee, Hak-Sung Kim
Enzyme-linked immunoassays based on an antibody-antigen interaction are widely used in biological and medical sciences. However, the conjugation of an enzyme to antibodies needs an additional chemical process, usually resulting in randomly cross-linked molecules and a loss of the binding affinity and enzyme activity. Herein, we present the development of an alkaline phosphatase-fused repebody as a new format of immuno-reagent for immunoassays. A repebody specifically binding to human TNF-α (hTNF-α) was selected through a phage display, and its binding affinity was increased up to 49 nM using a modular engineering approach...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915977/a-review-on-recent-patents-and-applications-of-inorganic-material-binding-peptides
#19
Veeranjaneyulu Thota, Carole C Perry
Over the past decade, significant progress has been made in the identification of novel material binding peptides having affinity to a wide range of target materials and their use in nanobiotechnological innovations. These material binding peptides (MBPs), also known as solid/ substance binding peptides (SBPs) can be isolated using combinatorial display technologies such as phage display (PD), surface display (cell, bacterial, yeast, mRNA) exhibit material specific selectivity and affinity towards a range of inorganic and organic nanomaterial surfaces including metals, metal oxides, minerals, semiconductors and biomolecules...
December 1, 2016: Recent Patents on Nanotechnology
https://www.readbyqxmd.com/read/27915247/genomic-diversity-of-bacteriophages-infecting-the-fish-pathogen-flavobacterium-psychrophilum
#20
Daniel Castillo, Mathias Middelboe
Bacteriophages infecting the fish pathogen Flavobacterium psychrophilum can potentially be used to prevent and control outbreaks of this bacterium in salmonid aquaculture. However, application of bacteriophages in disease control requires detailed knowledge on their genetic composition. To explore the diversity of F. pyschrophilum bacteriophages we have analyzed the complete genome sequences of 17 phages isolated from two distant geographic areas (Denmark and Chile), including the previously characterized temperate bacteriophage 6H...
December 2, 2016: FEMS Microbiology Letters
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