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Bacterial signal transduction

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https://www.readbyqxmd.com/read/27936405/identification-of-lipopolysaccharide-interacting-plasma-membrane-type-proteins-in-arabidopsis-thaliana
#1
Cornelius S Vilakazi, Ian A Dubery, Lizelle A Piater
Lipopolysaccharide (LPS) is an amphiphatic bacterial glycoconjugate found on the external membrane of Gram-negative bacteria. This endotoxin is considered as a microbe-associated molecular pattern (MAMP) molecule and has been shown to elicit defense responses in plants. Here, LPS-interacting proteins from Arabidopsis thaliana plasma membrane (PM)-type fractions were captured and identified in order to investigate those involved in LPS perception and linked to triggering of innate immune responses. A novel proteomics-based affinity-capture strategy coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for the enrichment and identification of LPS-interacting proteins...
November 30, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27935478/labeling-proteins-inside-living-cells-using-external-fluorophores-for-microscopy
#2
Kai Wen Teng, Yuji Ishitsuka, Pin Ren, Yeoan Youn, Xiang Deng, Pinghua Ge, Andrew S Belmont, Paul R Selvin
Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial enzyme which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG's to small ligands, with high efficiency (>85% of cells). The whole process, including recovery, takes 30 min, and the cell is ready to be imaged immediately. A variety of cell viability tests were performed after treatment with SLO to ensure that the cells have intact membranes, are able to divide, respond normally to signaling molecules, and maintains healthy organelle morphology...
December 9, 2016: ELife
https://www.readbyqxmd.com/read/27923921/high-resolution-ph-imaging-of-living-bacterial-cells-to-detect-local-ph-differences
#3
Yusuke V Morimoto, Nobunori Kami-Ike, Tomoko Miyata, Akihiro Kawamoto, Takayuki Kato, Keiichi Namba, Tohru Minamino
: Protons are utilized for various biological activities such as energy transduction and cell signaling. For construction of the bacterial flagellum, a type III export apparatus utilizes ATP and proton motive force to drive flagellar protein export, but the energy transduction mechanism remains unclear. Here, we have developed a high-resolution pH imaging system to measure local pH differences within living Salmonella enterica cells, especially in close proximity to the cytoplasmic membrane and the export apparatus...
December 6, 2016: MBio
https://www.readbyqxmd.com/read/27918078/nuclear-ca-2-signalling-in-arbuscular-mycorrhizal-and-actinorhizal-endosymbioses-on-the-trail-of-novel-underground-signals
#4
REVIEW
David G Barker, Mireille Chabaud, Guilia Russo, Andrea Genre
I. II. III. IV. References SUMMARY: Root endosymbioses are beneficial associations formed between terrestrial plants and either bacterial or fungal micro-organisms. A common feature of these intracellular symbioses is the requirement for mutual recognition between the two partners before host-regulated microbial entry. As part of this molecular dialogue, symbiosis-specific microbial factors set in motion a highly conserved plant signal transduction pathway, of which a central component is the activation of sustained nuclear Ca(2+) oscillations in target cells of the host epidermis...
December 5, 2016: New Phytologist
https://www.readbyqxmd.com/read/27914071/differential-radial-capillary-action-of-ligand-assay-dracala-for-high-throughput-detection-of-protein-metabolite-interactions-in-bacteria
#5
Mona W Orr, Vincent T Lee
Bacteria rely on numerous nucleotide second messengers for signal transduction such as cyclic AMP, cyclic-di-GMP, and cyclic-di-AMP. Although a number of receptors responsible for known regulated phenotypes have been established, the completeness of protein receptors in any given organism remains elusive. We have developed a method called differential radial capillary action of ligand assay (DRaCALA) that allows for an unbiased, systematic high-throughput screen for the detection of ligand binding proteins encoded by a genome...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27905546/transcriptome-analysis-confers-a-complex-disease-resistance-network-in-wild-rice-oryza-meyeriana-against-xanthomonas-oryzae-pv-oryzae
#6
Xiao-Jie Cheng, Bin He, Lin Chen, Su-Qin Xiao, Jian Fu, Yue Chen, Teng-Qiong Yu, Zai-Quan Cheng, Hong Feng
Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the devastating diseases of rice. It is well established that the wild rice Oryza meyeriana is immune to BB. In this study, the transcriptomic analysis was carried out by RNA sequencing of O. meyeriana leaves, inoculated with Xoo to understand the transcriptional responses and interaction between the host and pathogen. Totally, 57,313 unitranscripts were de novo assembled from 58.7 Gb clean reads and 14,143 unitranscripts were identified after Xoo inoculation...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901137/energy-transduction-and-signal-averaging-of-fluctuating-electric-fields-by-a-single-protein-ion-channel
#7
C Verdia-Baguena, V Gomez, J Cervera, P Ramirez, S Mafe
We demonstrate the electrical rectification and signal averaging of fluctuating signals using a biological nanostructure in aqueous solution: a single protein ion channel inserted in the lipid bilayer characteristic of cell membranes. The conversion of oscillating, zero time-average potentials into directional currents permits charging of a load capacitor to significant steady-state voltages within a few minutes in the case of the outer membrane porin F (OmpF) protein, a bacterial channel of Escherichia coli...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27885598/identification-of-ligand-receptor-interactions-ligand-molecular-arrays-spr-and-nmr-methodologies
#8
Christopher J Day, Lauren E Hartley-Tassell, Victoria Korolik
Despite many years of research into bacterial chemotaxis, the only well characterized system to date is that of E. coli. Even for E. coli, the direct ligand binding had been fully characterized only for aspartate and serene receptors Tar and Tsr. In 30 years since, no other direct receptor-ligand interaction had been described for bacteria, until the characterization of the C. jejuni aspartate and multiligand receptors (Hartley-Tassell et al. Mol Microbiol 75:710-730, 2010). While signal transduction components of many sensory pathways have now been characterized, ligand-receptor interactions remain elusive due to paucity of high-throughput screening methods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27862864/biological-relevance-of-volatile-organic-compounds-emitted-during-the-pathogenic-interactions-between-apple-plants-and-erwinia-amylovora
#9
Antonio Cellini, Giampaolo Buriani, Lorenzo Rocchi, Elena Rondelli, Stefano Savioli, Maria T Rodriguez Estrada, Simona M Cristescu, Guglielmo Costa, Francesco Spinelli
Volatile organic compounds emitted during the infection of apple (Malus pumila var. domestica) plants by Erwinia amylovora or Pseudomonas syringae pv. syringae were studied by gas chromatography-mass spectrometry and proton transfer reaction-mass spectrometry, and used to treat uninfected plants. Infected plants showed a disease specific emission of volatile organic compounds, including several bio-active compounds, such as hexenal isomers and 2,3-butanediol. Leaf growth promotion and a higher resistance to the pathogen, expressed as a lower bacterial growth and migration in plant tissues, were detected in plants exposed to volatile compounds from E...
November 15, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27832946/role-of-human-amnion-derived-mesenchymal-stem-cells-in-promoting-osteogenic-differentiation-by-influencing-p38-mapk-signaling-in-lipopolysaccharide-induced-human-bone-marrow-mesenchymal-stem-cells
#10
Yuli Wang, Hongxia Wu, Ming Shen, Siyang Ding, Jing Miao, Ning Chen
Periodontitis is a chronic inflammatory disease induced by bacterial pathogens,which not only affect connective tissue attachments but also cause alveolar bone loss.In this study, we investigated the anti-inflammatory effects of Human amnion-derived mesenchymal stem cells (HAMSCs) on human bone marrow mesenchymal stem cells (HBMSCs) under lipopolysaccharide (LPS)-induced inflammatory conditions. Proliferation levels were measured by flow cytometry and immunofluorescence staining of 5-ethynyl-2'-deoxyuridine (EdU)...
November 7, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27822540/identification-of-novel-host-interactors-of-effectors-secreted-by-salmonella-and-citrobacter
#11
Ryan L Sontag, Ernesto S Nakayasu, Roslyn N Brown, George S Niemann, Michael A Sydor, Octavio Sanchez, Charles Ansong, Shao-Yeh Lu, Hyungwon Choi, Dylan Valleau, Karl K Weitz, Alexei Savchenko, Eric D Cambronne, Joshua N Adkins
Many pathogenic bacteria of the family Enterobacteriaceae use type III secretion systems to inject virulence proteins, termed "effectors," into the host cell cytosol. Although host-cellular activities of several effectors have been demonstrated, the function and host-targeted pathways of most of the effectors identified to date are largely undetermined. To gain insight into host proteins targeted by bacterial effectors, we performed coaffinity purification of host proteins from cell lysates using recombinant effectors from the Enterobacteriaceae intracellular pathogens Salmonella enterica serovar Typhimurium and Citrobacter rodentium...
July 2016: MSystems
https://www.readbyqxmd.com/read/27818344/traf6-is-a-critical-factor-in-fish-immune-response-to-virus-infection
#12
Jingguang Wei, Shaoqing Zang, Meng Xu, Qiaojun Zheng, Xiuli Chen, Qiwei Qin
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adaptor molecule in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In our previous study, the molecular characteristics of EtTRAF6 (TRAF6 from Epinephelus tauvina), the tissue distributions, expression patterns after challenging with bacterial and viral pathogens were investigated. Here we identified EtTRAF6 as an important regulator of virus-triggered signaling pathway. Overexpression of EtTRAF6-ORF and truncated forms of EtTRAF6, including EtTRAF6-C (delete the MATH domain), EtTRAF6-N (delete the RING domain) and EtTRAF6-MATH, inhibited IFN-β activity strongly in grouper spleen (GS) cells...
November 3, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27803157/bacterial-energy-sensor-aer-modulates-the-activity-of-the-chemotaxis-kinase-chea-based-on-the-redox-state-of-the-flavin-cofactor
#13
Dipanjan Samanta, Joanne Widom, Peter P Borbat, Jack H Freed, Brian R Crane
Flagellated bacteria modulate their swimming behavior in response to environmental cues through the CheA/CheY signaling pathway. In addition to responding to external chemicals, bacteria also monitor internal conditions that reflect the availability of oxygen, light, and reducing equivalents - in a process termed ″Energy Taxis″. In E. coli, the transmembrane receptor Aer is the primary energy sensor for motility. Genetic and physiological data suggest that Aer monitors the electron transport chain through the redox state of its FAD cofactor...
November 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27799551/degradation-of-cyclic-diguanosine-monophosphate-by-a-hybrid-two-component-protein-protects-azoarcus-sp-strain-cib-from-toluene-toxicity
#14
Zaira Martín-Moldes, Blas Blázquez, Claudine Baraquet, Caroline S Harwood, María T Zamarro, Eduardo Díaz
Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that controls diverse functions in bacteria, including transitions from planktonic to biofilm lifestyles, virulence, motility, and cell cycle. Here we describe TolR, a hybrid two-component system (HTCS), from the β-proteobacterium Azoarcus sp. strain CIB that degrades c-di-GMP in response to aromatic hydrocarbons, including toluene. This response protects cells from toluene toxicity during anaerobic growth. Whereas wild-type cells tolerated a sudden exposure to a toxic concentration of toluene, a tolR mutant strain or a strain overexpressing a diguanylate cyclase gene lost viability upon toluene shock...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27796789/isolation-and-characterization-of-a-microrna-size-secretable-small-rna-in-streptococcus-sanguinis
#15
Ji-Woong Choi, Tae-Yub Kwon, Su-Hyung Hong, Heon-Jin Lee
MicroRNAs in eukaryotic cells are thought to control highly complex signal transduction and other biological processes by regulating coding transcripts, accounting for their important role in cellular events in eukaryotes. Recently, a novel class of bacterial RNAs similar in size [18-22 nucleotides (nt)] to microRNAs has been reported. Herein, we describe microRNAs, small RNAs from the oral pathogen Streptococcus sanguinis. The bacteria are normally present in the oral cavities and cause endocarditis by contaminating bloodstreams...
October 31, 2016: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27771745/stress-responses-of-acinetobacter-strain-y-during-phenol-degradation
#16
Johnson Lin
Quantification of gene expression of Acinetobacter strain Y under 1000 mg/l of phenol was investigated using qPCR and proteomic analyses. The results show that Acinetobacter strain Y utilized 100 % of phenol within 18 h of exposure. The results of qPCR and proteomic analyses demonstrate a sequential expression of phenol-degrading genes of Acinetobacter strain Y via the ortho-pathway followed by the β-ketoadipate pathway. Many stress-responsive proteins such as chaperones, chaperonins, porins and the enzymes involved in the signal transduction pathway were upregulated especially in the early stage...
October 22, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27758868/immunomodulatory-and-anti-inflammatory-activity-in-vitro-and-in-vivo-of-a-novel-antimicrobial-candidate
#17
Jlenia Brunetti, Giulia Roscia, Ilaria Lampronti, Roberto Gambari, Leila Quercini, Chiara Falciani, Luisa Bracci, Alessandro Pini
The synthetic antimicrobial peptide SET-M33 has strong activity against bacterial infections caused by Gram-negative bacteria. It is currently in preclinical development as a new drug to treat lung infections caused by Gram-negative bacteria. Here we report its strong anti-inflammatory activity in terms of reduced expression of a number of cytokines, enzymes, and signal transduction factors involved in inflammation triggered by LPS from Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli Sixteen cytokines and other major agents involved in inflammation were analyzed in macrophages and bronchial cells after stimulation with LPS and incubation with SET-M33...
December 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27756321/egfr-transactivation-contributes-to-neuroinflammation-in-streptococcus-suis-meningitis
#18
Xiao-Pei Yang, Ji-Yang Fu, Rui-Cheng Yang, Wen-Tong Liu, Tao Zhang, Bo Yang, Ling Miao, Bei-Bei Dou, Chen Tan, Huan-Chun Chen, Xiang-Ru Wang
BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic bacterial pathogen in both humans and animals, which can cause high morbidity and mortality. Meningitis is one of the major clinical manifestations of SS2 infection. However, the specific process of SS2 meningitis and its molecular mechanisms remain unclear. Epidermal growth factor receptor (EGFR) has been reported to initiate transduction of intracellular signals and regulate host inflammatory responses. Whether and how EGFR contributes to the development of S...
October 19, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27752708/the-biological-function-and-significance-of-cd74-in-immune-diseases
#19
Huiting Su, Ning Na, Xiaodong Zhang, Yong Zhao
CD74 (MHC class II invariant chain, Ii) is a non-polymorphic type II transmembrane glycoprotein. It is clear that, in addition to be an MHC class II chaperone, CD74 has a diversity of biological functions in physiological and pathological situations. CD74 also participates in other non-MHC II protein trafficking, such as angiotensin II type I receptor. In addition, CD74 is a cell membrane high-affinity receptor for macrophage migration inhibitory factor (MIF), D-dopachrome tautomerase (D-DT/MIF-2) and bacterial proteins...
October 17, 2016: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/27709103/the-tip-of-the-iceberg-on-the-roles-of-regulatory-small-rnas-in-the-virulence-of-enterohemorrhagic-and-enteropathogenic-escherichia-coli
#20
Shantanu Bhatt, Marisa Egan, Valerie Jenkins, Sarah Muche, Jihad El-Fenej
Enterohemorrhagic and enteropathogenic Escherichia coli are gastrointestinal pathogens that disrupt the intestinal microvilli to form attaching and effacing (A/E) lesions on infected cells and cause diarrhea. This pathomorphological trait is encoded within the pathogenicity island locus of enterocyte effacement (LEE). The LEE houses a type 3 secretion system (T3SS), which upon assembly bridges the bacterial cytosol to that of the host and enables the bacterium to traffic dozens of effectors into the host where they hijack regulatory and signal transduction pathways and contribute to bacterial colonization and disease...
2016: Frontiers in Cellular and Infection Microbiology
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