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Cutaneous immunohistochemistry

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https://www.readbyqxmd.com/read/28924059/pygopus2-expression-in-prostatic-adenocarcinoma-is-a-potential-risk-stratification-marker-for-psa-progression-following-radical-prostatectomy
#1
Kenneth R Kao, Paul Popadiuk, John Thoms, Satoko Aoki, Shahgul Anwar, Emily Fitzgerald, Phillip Andrews, Kim Voisey, Luis Gai, Satya Challa, Zhijian He, Paola Gonzales-Aguirre, Andrea Simmonds, Catherine Popadiuk
AIMS: Prostate cancer (PrCa) is the most frequently diagnosed non-cutaneous cancer in men. Without clear pathological indicators of disease trajectory at diagnosis, management of PrCa is challenging, given its wide-ranging manifestation from indolent to highly aggressive disease. This study examines the role in PrCa of the Pygopus (PYGO)2 chromatin effector protein as a risk stratification marker in PrCa. METHODS: RNA expression was performed in PrCa cell lines using Northern and RT-PCR analyses...
September 18, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28912854/comparative-proteomics-analysis-of-primary-cutaneous-amyloidosis
#2
Daxing Cai, Yang Li, Chunlei Zhou, Yulin Jiang, Jian Jiao, Lin Wu
Primary cutaneous amyloidosis (PCA) is a localized skin disorder that is characterized by the abnormal deposition of amyloid in the extracellular matrix (ECM) of the dermis. The pathogenesis of PCA is poorly understood. The objective of the present study was to survey proteome changes in PCA lesions in order to gain insight into the molecular basis and pathogenesis of PCA. Total protein from PCA lesions and normal skin tissue samples were extracted and analyzed using the isobaric tags for relative and absolute quantitation technique...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28905996/merkel-cells-and-meissner-s-corpuscles-in-human-digital-skin-display-piezo2-immunoreactivity
#3
Y García-Mesa, J García-Piqueras, B García, J Feito, R Cabo, J Cobo, J A Vega, O García-Suárez
The transformation of mechanical energy into electrical signals is the first step in mechanotransduction in the peripheral sensory nervous system and relies on the presence of mechanically gated ion channels within specialized sensory organs called mechanoreceptors. Piezo2 is a vertebrate stretch-gated ion channel necessary for mechanosensitive channels in mammalian cells. Functionally, it is related to light touch, which has been detected in murine cutaneous Merkel cell-neurite complexes, Meissner-like corpuscles and lanceolate nerve endings...
September 14, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28901563/analysis-of-serum-levels-and-cutaneous-expression-of-lipoprotein-a-in-38-patients-with-livedoid-vasculopathy
#4
Espinel Ds, Di Giacomo Tb, Pincelli Tp, Pereira Nv, Sotto Mn, Kirsner Rs, Criado Pr
BACKGROUND: Coagulation disorders contribute to the development of livedoid vasculopathy (LV). Elevated plasma levels of lipoprotein(a) [Lp(a)] are an independent risk factor for the development of cardiovascular disease and associated with hypercoagulable states. Increased serum Lp(a) levels have been reported in patients with LV and may have an important role in the pathogenesis of LV. OBJECTIVES: To investigate Lp(a) expression in skin lesions and circulating serum Lp(a) levels in patients with LV...
September 13, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28892183/histopathologic-features-of-cutaneous-leishmaniasis-and-use-of-cd1a-staining-for-amastigotes-in-old-world-and-new-world-leishmaniasis
#5
Lohini Sundharkrishnan, Jeffrey P North
BACKGROUND: Positive CD1a staining of leishmania has been reported in Old World leishmaniasis, but the sensitivity of such staining for other leishmania species is unknown. METHODS: A retrospective review was done on skin biopsies of proven cutaneous leishmaniasis based on histology, immunohistochemistry, culture, and/or PCR. We assessed the pattern of inflammation present and assessed for CD1a (MTB1 clone) positivity in amastigotes. Patients without a clearly documented travel history to delineate Old versus New World leishmaniasis and cases without tissue for CD1a staining were excluded...
September 11, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28879704/a-case-of-cutaneous-involvement-in-t-lymphoblastic-lymphoma-leukemia
#6
Ling Zhang, Yunxin Zeng, Na Cheng, Xiaoyun Liu, Xiaoqing Li, Bing Long, Dongjun Lin
BACKGROUND: Cutaneous involvement is more prevalent in B lymphoblastic lymphoma (B-LBL) than T lymphoblastic lymphoma (T-LBL). The authors describe a rare case of cutaneous involvement in a 25-year-old woman with T lymphoblastic lymphoma leukemia. METHODS: Hematologic investigation, bone marrow aspirate and biopsy, cytogenetic analysis and cutaneous lesion biopsy were performed. RESULTS: The patient achieved complete remission after induction therapy with the regimen of CHOEP + P, then received another CHOEP + P regimen...
September 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28857981/toll-like-receptor-7-staining-in-malignant-epithelial-tumors
#7
Regina Franz, Nathalie Ritter, Rüdiger Hein, Tilo Biedermann, Mohammed Al-Sisi, Kilian Eyerich, Natalie Garzorz-Stark, Christian Andres
BACKGROUND: As important players of the innate immune system, Toll-like receptors (TLRs) and their role for tumorigenesis have been in the focus of research. In particular TLR7 is an interesting candidate, as TLR7 agonists are broadly used for the treatment of cutaneous tumors. However, data addressing the baseline expression of TLR7 in both basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) on the protein level are lacking, while on the genomic level significantly elevated expression of TLR7 in SCC but not in BCC has been demonstrated...
October 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28851300/presence-of-immune-cells-low-tumor-proliferation-and-wild-type-braf-mutation-status-is-associated-with-a-favourable-clinical-outcome-in-stage-iii-cutaneous-melanoma
#8
Johan Falkenius, Hemming Johansson, Rainer Tuominen, Marianne Frostvik Stolt, Johan Hansson, Suzanne Egyhazi Brage
BACKGROUND: The variable prognosis in stage III cutaneous melanoma is partially due to unknown prognostic factors. Improved prognostic tools are required to define patients with an increased risk of developing metastatic disease who might benefit from adjuvant therapies. The aim was to examine if cellular immune markers in association with tumor proliferation rate and BRAF mutation status have an impact on prognosis in stage III melanoma. METHODS: We have used two sets of case series with stage III disease: 23 patients with short survival (≤ 13 months) and 19 patients with long survival (≥ 60 months)...
August 29, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28843624/sorafenib-stimulates-human-skin-type-mast-cell-degranulation-and-maturation
#9
Yukari Mizukami, Koji Sugawara, Yukimi Kira, Daisuke Tsuruta
BACKGROUND: Sorafenib is a multi-kinase inhibitor for treating advanced hepatocellular and renal cell carcinomas by targeting various types of receptors and signaling molecules, including vascular endothelial growth factor receptors, platelet-derived growth factor receptor, and Raf-1. Sorafenib may cause diverse cutaneous adverse reactions, including hand-foot reaction, facial and scalp eruptions, alopecia and pruritus. However, the mechanism of these adverse effects has not been well-investigated...
August 12, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28829805/expression-of-il-23-th17-related-cytokines-in-basal-cell-carcinoma-and-in-the-response-to-medical-treatments
#10
Cristina Pellegrini, Augusto Orlandi, Gaetana Costanza, Alessandro Di Stefani, Antonella Piccioni, Antonella Di Cesare, Andrea Chiricozzi, Amedeo Ferlosio, Ketty Peris, Maria Concetta Fargnoli
Several immune-related markers have been implicated in basal cell carcinoma (BCC) pathogenesis. The BCC inflammatory infiltrate is dominated by Th2 cytokines, suggesting a specific state of immunosuppression. In contrast, regressing BCC are characterized by a Th1 immune response with IFN-γ promoting a tumor suppressive activity. IL-23/Th17-related cytokines, as interleukin (IL)-17, IL-23 and IL-22, play a significant role in cutaneous inflammatory diseases, but their involvement in skin carcinogenesis is controversial and is poorly investigated in BCC...
2017: PloS One
https://www.readbyqxmd.com/read/28829109/adult-orbital-and-adnexal-xanthogranulomatous-disease
#11
Michael J Davies, Kevin Whitehead, Gary Quagliotto, Dominic Wood, Rajan S Patheja, Timothy J Sullivan
PURPOSE: Adult xanthogranulomatous disease of the orbit and ocular adnexa is a rare disease that can cause serious morbidity and mortality. Ophthalmologists are commonly the first clinicians to come in contact with affected patients and an understanding of the clinical features is essential. DESIGN: We present a retrospective case series of patients seen in the oculoplastic unit of a large tertiary referral hospital over a 20-year period. METHODS: The clinical files of 7 patients with adult xanthogranulomatous disease of the orbit and ocular adnexa were reviewed...
August 22, 2017: Asia-Pacific Journal of Ophthalmology
https://www.readbyqxmd.com/read/28802497/update-on-merkel-cell-carcinoma
#12
REVIEW
Paul W Harms
Merkel cell carcinoma (MCC) is a rare, aggressive cutaneous neuroendocrine malignancy. Merkel cell polyomavirus, a tumorigenic DNA virus, is present in most MCC tumors, with implications for tumor biology, diagnosis, and management. Merkel cell polyomavirus-negative tumors have a high burden of UV-signature mutations, similar to melanoma. The histopathologic diagnosis of MCC requires immunohistochemistry to exclude morphologically similar entities. Therapies for advanced disease are currently lacking. Here, the features of MCC are reviewed, including recent molecular discoveries with implications for improved therapy for advanced disease...
September 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/28802493/sentinel-lymph-nodes-in-cutaneous-melanoma
#13
REVIEW
Victor G Prieto
Examination of sentinel lymph nodes (SLNs) has probably become the most popular method of early staging of patients with cutaneous melanoma. SLNs are considered to be the most likely ones to contain metastatic deposits; thus, they can be examined in a more intense manner than in standard lymphadenectomy. Most processing protocols use formalin-fixed, paraffin-embedded sections stained with hematoxylin-eosin with the addition of immunohistochemistry. Approximately 20% of patients with cutaneous melanoma show melanoma cells in the SLNs...
September 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/28800143/immunohistochemistry-reveals-an-increased-proportion-of-myc-positive-cells-in-subcutaneous-panniculitis-like-t-cell-lymphoma-compared-with-lupus-panniculitis
#14
Sebastian Fernandez-Pol, Danielle De Stefano, Jinah Kim
BACKGROUND: Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a malignant primary cutaneous T-cell lymphoma that shares significant clinical, histopathologic, and immunophenotypic overlap with lupus erythematosus panniculitis (LEP). METHODS: We performed immunohistochemistry for the MYC oncoprotein on 23 cases of SPTCL (one CD8 negative) and 12 cases of LEP to evaluate if there are quantitative or qualitative differences in protein expression of this marker in these entities...
August 11, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28798001/giant-primary-sinonasal-mucosal-melanoma-a-rare-malignancy
#15
Mehtab Alam, Mohd Aslam, Piyush Kant Singh, Shahab Farkhund Hashmi, Syed Abrar Hasan
It is extremely rare to find a case of primary sinonasal mucosal melanoma, which is more aggressive and have poorer outcome than its cutaneous counterpart. This tumour is refractory to the treatment which includes wide surgical excision with or without adjuvant postoperative radiotherapy. We are reporting a case of 60 year old female who presented with right sided nasal mass and facial deformity, in whom the diagnosis of mucosal melanoma was made on histopathological examination and confirmed by immunohistochemistry...
May 2017: Gulf Journal of Oncology
https://www.readbyqxmd.com/read/28796379/review-of-the-current-medical-literature-and-assessment-of-current-utilization-patterns-regarding-human-papillomavirus-in-situ-hybridization-and-immunohistochemistry-in-dermatopathology
#16
REVIEW
Brandon R Litzner, Jason B Lee, Claudia I Vidal
Human papillomaviruses have been implicated in many cutaneous diseases. Practicing dermatopathologists often consider using immunohistochemistry and in situ hybridization to help clarify the histologic diagnosis, particularly in cases with borderline or non-diagnostic features. We reviewed the current evidence behind the use of these two techniques in dermatopathology. We identified only two studies utilizing the currently available immunohistochemical antibodies. We found more evidence regarding the use of in situ hybridization; however, the majority of this evidence focuses on diagnosing condylomas and other lesions of the genital skin...
August 10, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28796000/genomic-analysis-of-pigmented-epithelioid-melanocytomas-reveals-recurrent-alterations-in-prkar1a-and-prkca-genes
#17
Jarish N Cohen, Nancy M Joseph, Jeffrey P North, Courtney Onodera, Artur Zembowicz, Philip E LeBoit
Pigmented epithelioid melanocytoma (PEM) is a rare cutaneous melanocytic tumor first described as epithelioid blue nevus in patients with the Carney Complex (CC). PEM was among the first established examples of an intermediate class of melanocytic tumors, including atypical Spitz tumors, with frequent metastasis to lymph nodes but only rare extranodal spread. Sporadic and CC-associated PEM are essentially histologically indistinguishable. A subset of PEM shows loss of cytoplasmic expression of the protein kinase A regulatory subunit alpha (PRKAR1A), a tumor suppressor gene mutated in 70% of families with CC...
October 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28776087/efficacy-of-in-vivo-electroporation-mediated-il-10-gene-delivery-on-survival-of-skin-flaps
#18
S Morteza Seyed Jafari, Maziar Shafighi, Helmut Beltraminelli, Benedikt Weber, Ralph A Schmid, Thomas Geiser, Amiq Gazdhar, Robert E Hunger
Despite advances in understanding the underlying mechanisms of flap necrosis and improvement in surgical techniques, skin flap necrosis after reconstructive surgery remains a crucial issue. We investigated the efficacy of electroporation-mediated IL-10 gene transfer to random skin flap with an aim to accelerate wound healing and improve skin flap survival. Nine male Wistar rats (300-330 g) were divided in two groups (a) control group (n = 5), only surgery no gene transfer, and (b) experimental group, received electroporation-mediated IL-10 gene transfer 24 h before the surgery as prophylaxis (n = 4)...
August 3, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28761800/pancreatic-kaposiform-hemangioendothelioma-not-responding-to-sirolimus
#19
Paloma Junco Triana, Mariela Dore, Vanesa Cerezo Nuñez, Javier Gomez Jimenez, Miriam Ferrero Miguel, Mercedes González Díaz, Joan Novo Ricardo, Ane Andres, Manuel Lopez Santamaria, Juan Carlos Lopez-Gutierrez
Background  Kaposiform hemangioendothelioma (KHE) is a vascular tumor frequently associated with Kasabach-Merritt phenomenon (KMP), characterized by severe thrombocytopenia and consumptive coagulopathy. Visceral involvement in KHE is rare. In our recent experience, sirolimus has shown to be an effective treatment in cutaneous KHE, becoming indeed the treatment of choice in KMP. We report a case of pancreatic KHE associated with KMP and refractory to sirolimus. Case Report  A 4-month-old infant is referred for obstructive jaundice (10 mg/dL conjugated bilirubin) secondary to vascular pancreatic tumor...
January 2017: European Journal of Pediatric Surgery Reports
https://www.readbyqxmd.com/read/28756980/pkc-%C3%AE-attenuates-the-cancer-stem-cell-among-squamous-cell-carcinoma-cells-through-down-regulating-p63
#20
Dongmei Zhang, Mingjing Fu, Lingyan Li, Huan Ye, Zhiqi Song, Yongjun Piao
Protein kinase C delta (PKC-δ) has been identified as a tumor suppressor. However, the effects of PKC-δ on the cancer stem cells in squamous cell carcinomas (SCC) have not been clarified. The purpose of this study was to detect the regulation of PKC-δ on cancer stem cell among SSC cells and the role of p63 during the regulation. Immunohistochemistry of human cutaneous SCC tissues was performed to detect the expression of PKC-δ. After the human SCC13 cells infected by recombinant adenoviruses, the cell proliferation were determined...
July 14, 2017: Pathology, Research and Practice
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