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Biochemistry*

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https://www.readbyqxmd.com/read/28340465/dumbbell-dna-templated-cunps-as-a-nano-fluorescent-probe-for-detection-of-enzymes-involved-in-ligase-mediated-dna-repair
#1
Taiping Qing, Xiaoxiao He, Dinggeng He, Xiaosheng Ye, Jingfang Shangguan, Jinquan Liu, Baoyin Yuan, Kemin Wang
DNA repair processes are responsible for maintaining genome stability. Ligase and polynucleotide kinase (PNK) have important roles in ligase-mediated DNA repair. The development of analytical methods to monitor these enzymes involved in DNA repair pathways is of great interest in biochemistry and biotechnology. In this work, we reported a new strategy for label-free monitoring PNK and ligase activity by using dumbbell-shaped DNA templated copper nanoparticles (CuNPs). In the presence of PNK and ligase, the dumbbell-shaped DNA probe (DP) was locked and could resist the digestion of exonucleases and then served as an efficient template for synthesizing fluorescent CuNPs...
March 21, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28337237/relationship-between-helicobacter-pylori-infection-and-celiac-disease-a-cross-sectional-study-and-a-brief-review-of-the-literature
#2
Sebahat Basyigit, Oktay Unsal, Metin Uzman, Ferdane Sapmaz, Ozlem Ceylan Dogan, Ayse Kefeli, Zeliha Asilturk, Abdullah Ozgur Yeniova, Yasar Nazligul
INTRODUCTION: Whether Helicobacter pylori triggers celiac disease (CD) or protects against CD is currently the subject of research. In the literature, there are epidemiologic studies that have reported conflicting results regarding the association between H. pylori and CD. AIM: To compare the prevalence of CD autoantibody positivity and the levels of CD autoantibodies between H. pylori-positive and H. pylori-negative subjects. MATERIAL AND METHODS: This study was prospectively designed and included 240 dyspeptic patients who underwent upper gastrointestinal endoscopy with gastric and duodenal biopsies...
2017: Przegla̜d Gastroenterologiczny
https://www.readbyqxmd.com/read/28336071/sensitive-protein-detection-and-quantification-in-paper-based-microfluidics-for-the-point-of-care
#3
Caitlin E Anderson, Kamal G Shah, Paul Yager
The design of appropriate diagnostic assays for the point of care requires development of suitable biosensors, detection methods, and diagnostic platforms for sensitive, quantitative detection of biological analytes. Protein targets in particular are especially challenging to detect quantitatively and sensitively due to the lack of amplification strategies akin to nucleic acid amplification. However, recent advances in transducer and biosensor design, new detection labels, and paper-based microfluidics may realize the goal of sensitive, fast, portable, and low-cost protein detection...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28335962/combining-anova-pca-with-pochemon-to-analyse-micro-organism-development-in-a-polymicrobial-environment
#4
Brigitte P Geurts, Anne H Neerincx, Samuel Bertrand, Manja A A P Leemans, Geert J Postma, Jean-Luc Wolfender, Simona M Cristescu, Lutgarde M C Buydens, Jeroen J Jansen
Revealing the biochemistry associated to micro-organismal interspecies interactions is highly relevant for many purposes. Each pathogen has a characteristic metabolic fingerprint that allows identification based on their unique multivariate biochemistry. When pathogen species come into mutual contact, their co-culture will display a chemistry that may be attributed both to mixing of the characteristic chemistries of the mono-cultures and to competition between the pathogens. Therefore, investigating pathogen development in a polymicrobial environment requires dedicated chemometric methods to untangle and focus upon these sources of variation...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28335417/dna-sequencing-sensors-an-overview
#5
REVIEW
Jose Antonio Garrido-Cardenas, Federico Garcia-Maroto, Jose Antonio Alvarez-Bermejo, Francisco Manzano-Agugliaro
The first sequencing of a complete genome was published forty years ago by the double Nobel Prize in Chemistry winner Frederick Sanger. That corresponded to the small sized genome of a bacteriophage, but since then there have been many complex organisms whose DNA have been sequenced. This was possible thanks to continuous advances in the fields of biochemistry and molecular genetics, but also in other areas such as nanotechnology and computing. Nowadays, sequencing sensors based on genetic material have little to do with those used by Sanger...
March 14, 2017: Sensors
https://www.readbyqxmd.com/read/28333641/droplet-size-aware-high-level-synthesis-for-micro-electrode-dot-array-digital-microfluidic-biochips
#6
Zipeng Li, Kelvin Yi-Tse Lai, Po-Hsien Yu, Krishnendu Chakrabarty, Tsung-Yi Ho, Chen-Yi Lee
A digital microfluidic biochip (DMFB) is an attractive technology platform for automating laboratory procedures in biochemistry. In recent years, DMFBs based on a microelectrode-dot-array (MEDA) architecture have been demonstrated. However, due to the inherent differences between today's DMFBs and MEDA, existing synthesis solutions for biochemistry mapping cannot be utilized for MEDA biochips. We present the first synthesis approach that can be used for MEDA biochips. We first present a general analytical model for droplet velocity and validate it experimentally using a fabricated MEDA biochip...
March 21, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28332350/suksin-lee-the-first-ph-d-and-full-time-professor-of-biochemistry-in-korea
#7
EDITORIAL
Byung Il Yeh
No abstract text is available yet for this article.
May 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28330724/treatment-effects-and-mechanisms-of-yujin-powder-on-rat-model-of-large-intestine-dampness-heat-syndrome
#8
Wanling Yao, Chaoxue Yang, Yanqiao Wen, Wangdong Zhang, Xiaosong Zhang, Qi Ma, Peng Ji, Yongli Hua, Yanming Wei
ETHNOPHARMACOLOGICAL RELEVANCE: Yujin Powder (YJP), an old prescription, is one of the most classical prescription for treating the large intestine dampness-heat syndrome (LIDHS). However, its potential modern pharmacological mechanisms remain unclear. AIM OF THE STUDY: The present study was designed to explore the essence of LIDHS and treatment mechanisms of the YJP on the LIDHS. METHODS: The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment (HTHE), drinking and intraperitoneal injection of Escherichia coli...
March 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28330557/loss-of-anthocyanins-and-modification-of-the-anthocyanin-profiles-in-grape-berries-of-malbec-and-bonarda-grown-under-high-temperature-conditions
#9
Inés de Rosas, María Teresa Ponce, Emiliano Malovini, Leonor Deis, Bruno Cavagnaro, Pablo Cavagnaro
Malbec and Bonarda are the two most widely cultivated grape varieties in Argentina, and their derived red wines are recognized worldwide, being their intense color a major quality trait. The temperature during fruit ripening conditions berries color intensity. In the main viticulture region of Malbec and Bonarda a 2-3°C increase in temperature has been predicted for the upcoming years as consequence of the global climate change. In the present study, this predicted temperature raise was simulated under field-crop conditions, and its effect on anthocyanin pigmentation in berries of Malbec and Bonarda was monitored by HPLC analysis throughout the ripening process, in two growing seasons...
May 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28329754/biochemistry-a-wine-induced-breakdown
#10
Mirjam Czjzek
No abstract text is available yet for this article.
March 22, 2017: Nature
https://www.readbyqxmd.com/read/28329491/congenital-erythropoietic-porphyria-gunther-disease-long-term-follow-up-of-a-case-and-review
#11
Matthew Howard, Anthony Hall, Donald Ramsay
Patients with the rare genodermatosis congenitalerythropoietic porphyria (CEP, Gunther disease)develop erosions and scarring on sun-exposedsites caused by phototoxin mediated damage.Compromised skin barrier function places patientsat higher risk of infection and long term sequelaeinclude scarring. We report a long term follow up ofa 60 year old patient born with CEP and provide anextensive literature review of CEP including recentupdates on potential management options. Multiplepatient interviews and collection of biochemistry datawere conducted for the case discussion...
February 15, 2017: Dermatology Online Journal
https://www.readbyqxmd.com/read/28327966/de-novo-transcriptome-assemblies-of-four-xylem-sap-feeding-insects
#12
Erica E Tassone, Charles C Cowden, S J Castle
Background: Spittle bugs and sharpshooters are well-known xylem sap-feeding insects and vectors of the phytopathogenic bacterium Xylella fastidiosa (Wells), a causal agent of Pierce's disease of grapevines and other crop diseases. Specialized feeding on nutrient-deficient xylem sap is relatively rare among insect herbivores, and only limited genomic and transcriptomic information has been generated for xylem-sap feeders. To develop a more comprehensive understanding of biochemical adaptations and symbiotic relationships that support survival on a nutritionally austere dietary source, transcriptome assemblies for three sharpshooter species and one spittlebug species were produced...
February 24, 2017: GigaScience
https://www.readbyqxmd.com/read/28326041/influence-of-estimated-training-status-on-anti-and-pro-oxidant-activity-nitrite-concentration-and-blood-pressure-in-middle-aged-and-older-women
#13
André M Jacomini, Danielle da Silva Dias, Janaina de Oliveira Brito, Roberta F da Silva, Henrique L Monteiro, Susana Llesuy, Kátia De Angelis, Sandra L Amaral, Anderson S Zago
The purpose of this study was to compare the association between anti and pro-oxidant activity, nitrite concentration, and blood pressure (BP) in middle-aged and older women with different levels of estimated training status (TS). The sample consisted of 155 females (50-84 years) who were submitted to a physical examination to evaluate estimated TS through the "Functional Fitness Battery Test," BP measurements, and plasma blood samples to evaluate pro-oxidant and antioxidant activity and nitrite concentrations...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28325705/biochemical-hydrological-and-mechanical-behaviors-of-high-food-waste-content-msw-landfill-preliminary-findings-from-a-large-scale-experiment
#14
Liang-Tong Zhan, Hui Xu, Yun-Min Chen, Fan Lü, Ji-Wu Lan, Li-Min Shao, Wei-An Lin, Pin-Jing He
A large-scale bioreactor experiment lasting for 2years was presented in this paper to investigate the biochemical, hydrological and mechanical behaviors of high food waste content (HFWC) MSW. The experimental cell was 5m in length, 5m in width and 7.5m in depth, filled with unprocessed HFWC-MSWs of 91.3 tons. In the experiment, a surcharge loading of 33.4kPa was applied on waste surface, mature leachate refilling and warm leachate recirculation were performed to improve the degradation process. In this paper, the measurements of leachate quantity, leachate level, leachate biochemistry, gas composition, waste temperature, earth pressure and waste settlement were presented, and the following observations were made: (1) 26...
March 18, 2017: Waste Management
https://www.readbyqxmd.com/read/28325321/biosynthesis-of-agar-in-red-seaweeds-a-review
#15
REVIEW
Wei-Kang Lee, Yi-Yi Lim, Adam Thean-Chor Leow, Parameswari Namasivayam, Janna Ong Abdullah, Chai-Ling Ho
Agar is a jelly-like biopolymer synthesized by many red seaweeds as their major cell wall component. Due to its excellent rheological properties, it has been exploited commercially for applications in food, cosmetic, pharmaceutical, biomedical and biotechnology industries. Despite its multiple uses, the biosynthesis of this phycocolloid is not fully understood. The current knowledge on agar biosynthesis is inferred from plant biochemistry and putative pathways for ulvan and alginate biosynthesis in green and brown seaweeds, respectively...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28323756/tdf-associated-renal-tubular-dysfunction-non-invasive-assessment-of-mitochondrial-injury
#16
Ryan Samuels, Carla Roca Bayerri, John A Sayer, D Ashley Price, Brendan Ai Payne
OBJECTIVE: To determine whether TDF-associated renal tubular dysfunction is associated with evidence of mitochondrial injury in urine. DESIGN: Single centre cross-sectional observational study of HIV positive outpatients. METHODS: Biochemistry was performed on paired serum and urine samples. Mitochondrial DNA (mtDNA) was studied by real-time PCR and long-range PCR on cellular fractions of urine. RESULTS: 48 subjects were enrolled of whom half were TDF-treated...
March 18, 2017: AIDS
https://www.readbyqxmd.com/read/28322741/evidence-of-k-homeostasis-disruption-in-cellular-dysfunction-triggered-by-7-ketocholesterol-24s-hydroxycholesterol-and-tetracosanoic-acid-c24-0-in-158n-murine-oligodendrocytes
#17
Maryem Bezine, Meryam Debbabi, Thomas Nury, Rym Ben-Khalifa, Mohammad Samadi, Mustapha Cherkaoui-Malki, Anne Vejux, Jérôme de Sèze, Thibault Moreau, Mohamed El-Ayeb, Gérard Lizard
Imbalance in the homeostasis of K(+) ions has been reported to contribute to the pathogenesis of neurodegenerative diseases. 7-ketocholesterol (7KC), 24S-hydroxycholesterol (24S-OHC), and tetracosanoic acid (C24:0), often found at increased levels in patients with Alzheimer's disease, Multiple Sclerosis and X-ALD, are able to trigger numerous nerve cell dysfunctions. We therefore studied the impact of 7KC, 24S-OHC, and C24:0 on 158N murine oligodendrocytes, and determined their impact on K(+) homeostasis. The effects of 7KC, 24S-OHC and C24:0 on lipid membrane organization and membrane potential were examined with merocyanine 540 (MC540) and bis-(1,3-diethylthiobarbituric acid) trimethine oxonol (DiSBAC2(3)), respectively...
March 17, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28322061/potential-biomarkers-for-monitoring-the-toxicity-of-long-term-exposure-to-atrazine-in-rat-by-metabonomic-analysis
#18
Xiao-Feng Zhang, Chong-Hua Zhang, Jing Zheng, Long-Xue Li, Tian-Qi Geng, Yang Zhang
1. Herbicide atrazine (ATR) poses harmful effects on human health. The purpose of this study is to study potential biomarkers used for monitoring the toxic effects after chronic exposure to ATR by studying urine metabolites. 2. Rats were assigned into clinical chemistry and metabonomics arms, and each arm was divided into low-dose, high-dose and control groups. ATR was administered to rats along with their feed. At the end of 16, 20 and 24 weeks, clinical parameters and histopathologic changes was assessed to monitor the toxic effects...
March 21, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28320872/the-drosophila-daxx-like-protein-dlp-cooperates-with-asf1-for-h3-3-deposition-and-heterochromatin-formation
#19
Catherine Fromental-Ramain, Philippe Ramain, Ali Hamiche
Histone variants are non-allelic isoforms of canonical histones and they are deposited, in contrast to canonical histones, in a replication-independent (RI) manner. RI deposition of H3.3, a histone variant from the H3.3 family, is mediated in mammals by distinct pathways involving either the histone regulator A (HIRA) complex or the death-associated protein (DAXX)/α-thalassemia X-linked mental retardation protein (ATRX) complex. Here, we investigated the function of Drosophila DAXX Like Protein (DLP) by using both fly genetics approaches and protein biochemistry...
March 20, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28320290/do-external-or-internal-factors-lead-to-tumor-development-it-is-still-unknown
#20
COMMENT
V N Manskikh
Arguments supporting the "bad luck" hypothesis presented by C. Tomasetti and B. Vogelstein ((2015) Science, 347, 78-81) and A. V. Lichtenstein ((2017) Biochemistry (Moscow), 82, 75-80) are critically discussed. Those arguments are not sufficient for recognition of the "bad luck" hypothesis and the leading role of internal factors in spontaneous tumor development.
January 2017: Biochemistry. Biokhimii︠a︡
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