keyword
MENU ▼
Read by QxMD icon Read
search

Biochemistry*

keyword
https://www.readbyqxmd.com/read/28636874/next-generation-matrix-metalloproteinase-inhibitors-novel-strategies-bring-new-prospects
#1
REVIEW
Maxim Levin, Yael Udi, Inna Solomonov, Irit Sagi
Enzymatic proteolysis of cell surface proteins and extracellular matrix (ECM) is critical for tissue homeostasis and cell signaling. These proteolytic activities are mediated predominantly by a family of proteases termed matrix metalloproteinases (MMPs). The growing evidence in recent years that ECM and non-ECM bioactive molecules (e.g., growth factors, cytokines, chemokines, on top of matrikines and matricryptins) have versatile functions redefines our view on the roles matrix remodeling enzymes play in many physiological and pathological processes, and underscores the notion that ECM proteolytic reaction mechanisms represent master switches in the regulation of critical biological processes and govern cell behavior...
June 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28635337/population-based-genetic-study-of-%C3%AE-thalassemia-mutations-in-mardan-division-khyber-pakhtunkhwa-province-pakistan
#2
Raj Muhammad, Muhammad Shakeel, Shoaib U Rehman, Muhammad A Lodhi
β-Thalassemia (β-thal) is the most prevalent hereditary blood disorder in Pakistan with a carrier rate of 5.0-8.0%. The homozygous affected children require frequent blood transfusions for their survival. This autosomal recessive disease can only be prevented through awareness programs, carrier screening, mutation detection, genetic counseling and prenatal diagnosis (PND). The present study aimed to determine the prevalence of various mutations causing β-thal and also to detect carriers of these mutations in families living in the Mardan Division, Khyber Pakhtunkhwa (KP) Province, Pakistan...
June 21, 2017: Hemoglobin
https://www.readbyqxmd.com/read/28635300/pyridine-dinucleotides-from-molecules-to-man
#3
Joshua P Fessel, William M Oldham
SIGNIFICANCE: Pyridine dinucleotides, NAD and NADP, were discovered over 100 years ago as necessary cofactors for fermentation in yeast extracts. Since that time, these molecules have been recognized as fundamental players in a variety of cellular processes, including energy metabolism, redox homeostasis, cellular signaling, and gene transcription, among many others. Given their critical role as mediators of cellular responses to metabolic perturbations, it is unsurprising that dysregulation of NAD and NADP metabolism has been associated with the pathobiology of many chronic human diseases...
June 21, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28634639/the-curious-case-of-achromobacter-eurydice-a-gram-variable-pleomorphic-bacterium-associated-with-european-foulbrood-disease-in-honeybees
#4
REVIEW
Silvio Erler, Oleg Lewkowski, Anja Poehlein, Eva Forsgren
Honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. Bacterial pathogens in particular lead to destructive infections of the brood. European foulbrood is caused by the bacterium Melissococcus plutonius in combination with several other Gram-positive bacteria (Achromobacter eurydice, Bacillus pumilus, Brevibacillus laterosporus, Enterococcus faecalis, Paenibacillus alvei, Paenibacillus dendritiformis) involved as secondary invaders following the initial infection...
June 21, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28634146/sub-chronic-oral-toxicity-of-cuminum-cyminum-l-s-essential-oil-in-female-wistar-rats
#5
Mohsen Taghizadeh, Seyed Naser Ostad, Zatollah Asemi, Mohaddese Mahboubi, Sara Hejazi, Reza Sharafati-Chaleshtori, Aliakbar Rashidi, Hosein Akbari, Nasrin Sharifi
The current study was performed to evaluate the toxicity of Cuminum cyminum L. (C. cyminum)'s essential oil after 23 days and 45 days of repeated oral administration in female Wistar rats. A total of 80 healthy female Wistar rats were randomly selected and divided into 4 groups. The rats were gavaged with C. cyminum's essential oil at dose levels of 0, 250, 500 and 1000 mg/kg/day. Clinical signs, body weight, hematology, serum biochemistry and organ histopathology were assessed once after 23 days and again after 45 days passed from the start of the intervention...
June 17, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28632747/selective-cyclooxygenase-inhibition-by-sc-560-improves-hepatopulmonary-syndrome-in-cirrhotic-rats
#6
Ching-Chih Chang, Wen-Shin Lee, Hsian-Guey Hsieh, Chiao-Lin Chuang, Hui-Chun Huang, Fa-Yauh Lee, Shou-Dong Lee
OBJECTIVE: Hepatopulmonary syndrome (HPS) is characterized by hypoxia in patients with chronic liver disease. The mechanism of HPS includes pulmonary vasodilatation, inflammation, and angiogenesis. Prostaglandins synthesized by cyclooxygenases (COX) participate in vascular responsiveness, inflammation and angiogenesis, which can be modulated by COX inhibitors. We therefore evaluated the impact of COX inhibition in rats with common bile duct ligation (CBDL)-induced liver cirrhosis and HPS...
2017: PloS One
https://www.readbyqxmd.com/read/28631225/definition-of-the-upper-reference-limit-for-thyroglobulin-antibodies-according-to-the-national-academy-of-clinical-biochemistry-guidelines-comparison-of-eleven-different-automated-methods
#7
F D'Aurizio, P Metus, A Ferrari, B Caruso, R Castello, D Villalta, A Steffan, K Gaspardo, F Pesente, N Bizzaro, E Tonutti, S Valverde, C Cosma, M Plebani, R Tozzoli
PURPOSE: In the last two decades, thyroglobulin autoantibodies (TgAb) measurement has progressively switched from marker of thyroid autoimmunity to test associated with thyroglobulin (Tg) to verify the presence or absence of TgAb interference in the follow-up of patients with differentiated thyroid cancer. Of note, TgAb measurement is cumbersome: despite standardization against the International Reference Preparation MRC 65/93, several studies demonstrated high inter-method variability and wide variation in limits of detection and in reference intervals...
December 2017: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/28630704/the-effects-of-a-post-exercise-carbohydrate-and-protein-supplement-on-repeat-performance-serum-chemistry-insulin-and-glucagon-in-competitive-weight-pulling-dogs
#8
Christopher W Frye, Gretchen M VanDeventer, Gina K Dinallo, Jennifer A Poplarski, Sabine Mann, Ella Pittman, Brian M Zanghi, Joseph J Wakshlag
The physiological demands of weight-pulling dogs have yet to be investigated. Two groups of competitive weight-pulling dogs both underwent two identical pull series 3 h apart. The control group (n 8) was compared with a group fed a rapidly digestible carbohydrate and protein supplement after the first pull series (n 9). Blood was drawn before and after each pull series as well as at 15 and 30 min after the first pull series finished. Biochemistry values remained unremarkable throughout the study in both groups regardless of supplementation or exercise over time...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/28630703/energy-requirements-for-growth-in-the-yorkshire-terrier
#9
Janet E Alexander, Alison Colyer, Penelope J Morris
The 2006 National Research Council (NRC) equation calculating puppy energy requirements does not account for reported breed differences in growth pattern. Energy requirements of toy breed puppies are unknown and it is unclear whether feeding guidelines should differ between breeds. Energy requirements of Yorkshire terrier (YT) puppies were observed over their first year of life and compared with those predicted by the NRC and those previously observed in large (Labrador retriever) and medium (miniature Schnauzer; MS) breed puppies...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/28630007/rna-localization-making-its-way-to-the-center-stage
#10
REVIEW
Ashley Chin, Eric Lécuyer
Cells are highly organized entities that rely on intricate addressing mechanisms to sort their constituent molecules to precise subcellular locations. These processes are crucial for cells to maintain their proper organization and carry out specialized functions in the body, while genetic perturbations that clog up these addressing systems can contribute to disease aetiology. The trafficking of RNA molecules represents an important layer in the control of cellular organization, a process that is both highly prevalent and for which features of the regulatory machineries have been deeply conserved evolutionarily...
June 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28629835/ruthenium-complex-exerts-antineoplastic-effects-that-are-mediated-by-oxidative-stress-without-inducing-toxicity-in-walker-256-tumor-bearing-rats
#11
Carlos Eduardo Alves de Souza, Helen de Morais Alves de Souza, Maria Carolina Stipp, Claudia Rita Corso, Claudia Martins Galindo, Carolina Riverin Cardoso, Rosangela Locatelli Dittrich, Edneia Amancio de Souza Ramos Cavalieri, Giseli Klassen, Rose Maria Carlos, Sílvia Maria Suter Correia Cadena, Alexandra Acco
The present study evaluated the in vivo antitumor effects and toxicity of a new Ru(II) compound, cis-(Ru[phen]2[ImH]2)(2+) (also called RuphenImH [RuC]), against Walker-256 carcinosarcoma in rats. After subcutaneous inoculation of Walker-256 cells in the right pelvic limb, male Wistar rats received 5 or 10mg·kg(-1) RuC orally or intraperitoneally (i.p.) every 3 days for 13 days. A positive control group (2mg·kg(-1) cisplatin) and negative control group (vehicle) were also used. Tumor progression was checked daily...
June 16, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28627759/vibrational-biospectroscopy-characterises-biochemical-differences-between-cell-types-used-for-toxicological-investigations-and-identifies-alterations-induced-by-environmental-contaminants
#12
Kelly A Heys, Richard F Shore, M Glória Pereira, Francis L Martin
The use of cell-based assays is essential in reducing the number of vertebrates used in the investigation of chemical toxicities and in regulatory toxicology assessment. An important factor in obtaining meaningful results which can be accurately extrapolated is the use of biologically appropriate cell lines. In this preliminary study, ATR-FTIR spectroscopy with multivariate analysis was used to assess the fundamental biomolecular differences between a commonly used cell line, MCF-7 cells, and an environmentally relevant cell line derived from Mallard (Anas platyrhynchos) dermal fibroblasts...
June 19, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28627731/cell-migration-analysis-a-low-cost-laboratory-experiment-for-cell-and-developmental-biology-courses-using-keratocytes-from-fish-scales
#13
Daniel Prieto, Gonzalo Aparicio, Jose R Sotelo-Silveira
Cell and developmental processes are complex, and profoundly dependent on spatial relationships that change over time. Innovative educational or teaching strategies are always needed to foster deep comprehension of these processes and their dynamic features. However, laboratory exercises in cell and developmental biology at the undergraduate level do not often take into account the time dimension. In this article, we provide a laboratory exercise focused in cell migration, aiming to stimulate thinking in time and space dimensions through a simplification of more complex processes occurring in cell or developmental biology...
June 19, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28627343/-study-on-the-role-of-autophagy-in-heme-oxygenase-1-preventing-hepatic-ischemia-reperfusion-injury-in-rats
#14
Sheng Lan, Jintai Li, Yi Liu
OBJECTIVE: To identify the role of autopahgy in the protective mechanism of heme oxygenase 1 (HO-1) against hepatic ischemia/reperfusion (I/R) injury. METHODS: Forty healthy male Sprague-Dawley (SD) rats were randomly (random number table) divided into five groups (n = 8 in each group), namely sham group, model group, cobalt protoporphyrin (CoPP) group, zinc protoporphyrin (ZnPP) group and 6-amino-3-methylpurine (3-MA) group. Partial hepatic I/R model was established by clamping the pedicles of left and median lobes for 1 hour and reopening for 6 hours in rats, and the rats in sham group were only received celiotomp without hepatic I/R...
March 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28626960/whole-blood-taurine-concentrations-in-cats-with-intestinal-disease
#15
A Kathrani, A J Fascetti, J A Larsen, C Maunder, E J Hall
BACKGROUND: Increased delivery of taurine-conjugated bile acids to the distal bowel can lead to dysbiosis resulting in colitis in mouse models of inflammatory bowel disease. A similar situation also could occur in cats with intestinal disease and might therefore result in decreased whole-body taurine concentration. HYPOTHESIS/OBJECTIVES: To determine whether whole-blood taurine concentrations are decreased at the time of diagnosis in cats with intestinal disease and to correlate concentrations with clinical and laboratory variables...
June 19, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28623915/safety-pharmacology-and-subchronic-toxicity-of-jinqing-granules-in-rats
#16
Xuerong Zhou, Qian Rong, Min Xu, Yuanli Zhang, Qi Dong, Yuanling Xiao, Qiji Liu, Helin Chen, Xiaoyu Yang, Kaisheng Yu, Yinglun Li, Ling Zhao, Gang Ye, Fei Shi, Cheng Lv
BACKGROUND: Jinqing granules which are made of a mixture extract that contains Radix Tinosporae and Canarii fructus in proportions according to a longstanding formula have a good effect on the prevention and treatment of gastric ulcer disease. It has not been through safety through systematic toxicological studies, however. To provide basis for clinical application, we performed safety pharmacology and subchronic toxicity experiments in specific pathogen-free Sprague-Dawley rats. RESULTS: In safety pharmacology experiments, Jinqing granules had no evident adverse effects on the central nervous, cardiovascular, or respiratory systems...
June 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28623662/advances-in-the-analysis-of-water-and-wastewater-samples-using-various-sensing-protocols-and-microfluidic-devices-based-on-pad-and-%C3%AE-tas-systems
#17
Krzysztof Piaskowski, Renata Świderska-Dąbrowska, Aleksandra Kaleniecka, Paweł K Zarzycki
The main goal of this review is to summarize practical approaches concerning the application of microfluidic systems for the analysis of various biomarkers and pollutants, as well as microbes, in water and wastewater matrixes. This problem involves multidisciplinary expertise combining research knowledge from various areas, including wet chemistry, biochemistry, physical chemistry, molecular biology, genetics, signal processing, microelectronics material science, and separation science. It has been documented that fairly primitive but fast and inexpensive screening methods involving paper-based analytical devices (PADs) and micro total analytical systems (μTAS) can be considered as serious alternatives to their more advanced counterparts such as GC, HPLC, and capillary electrophoresis coupled to various sophisticated detectors (e...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623170/nicotinic-acetylcholine-receptor-inhibitors-derived-from-snake-and-snail-venoms
#18
REVIEW
Sebastien Dutertre, Annette Nicke, Victor Tsetlin
The nicotinic acetylcholine receptor (nAChR) represents the prototype of ligand-gated ion channels. It is vital for neuromuscular transmission and an important regulator of neurotransmission. A variety of toxic compounds derived from diverse species target this receptor and have been of elemental importance in basic and applied research. They enabled milestone discoveries in pharmacology and biochemistry ranging from the original formulation of the receptor concept, the first isolation and structural analysis of a receptor protein (the nAChR) to the identification, localization, and differentiation of its diverse subtypes and their validation as a target for therapeutic intervention...
June 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28622707/coordination-between-leaf-co2-diffusion-and-rubisco-properties-allows-maximizing-photosynthetic-efficiency-in-limonium-species
#19
Jeroni Galmés, Arántzazu Molins, Jaume Flexas, Miquel À Conesa
High photosynthetic efficiency intrinsically demands tight coordination between traits related to CO2 diffusion capacity and leaf biochemistry. Although this coordination constitutes the basis of existing mathematical models of leaf photosynthesis, it has been barely explored among closely related species, which could reveal rapid adaptation clues in the recent past. With this aim, we characterized the photosynthetic capacity of twelve species of Limonium, possessing contrasting Rubisco catalytic properties, grown under optimal (WW) and extreme drought conditions (WD)...
June 16, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28621886/review-of-emerging-metabolomic-tools-and-resources-2015-2016
#20
REVIEW
Biswapriya Biswavas Misra, Johannes F Fahrmann, Dmitry Grapov
Data processing and analysis are major bottlenecks in high-throughput metabolomic experiments. Recent advancements in data acquisition platforms are driving trends toward increasing data size (e.g. petabyte scale) and complexity (multiple omic platforms). Improvements in data analysis software and in silico methods are similarly required to effectively utilize these advancements and link the acquired data with biological interpretations. Herein we provide an overview of recently developed and freely-available metabolomic tools, algorithms, databases and data analysis frameworks...
June 16, 2017: Electrophoresis
keyword
keyword
3481
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"